Transcapillary PO2 Gradients in Contracting Muscle of Rat Model of Sepsis.

IF 1.8 4区医学 Q3 PERIPHERAL VASCULAR DISEASE

Journal of Vascular Research Pub Date : 2025-07-24 DOI:10.1159/000547568

Narumi Fukuzaki, Kazuki Hotta, Kota Izawa, Naoki Hitosugi, Miki Sakamoto, Rin Kataoka, Shuri Arai, Kentaro Kamiya, Atsuhiko Matsunaga

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引用次数: 0

Abstract

Objectives: This study aimed to evaluate the effect of cecal ligation and puncture (CLP)-induced sepsis on transcapillary PO2 gradients (ΔPO2) in contracting skeletal muscle.

Methods: Male Wistar rats (n = 16, 10 weeks old) were randomly assigned to sham or CLP groups. Seven days after surgery, microvascular (PO2mv) and interstitial (PO2is) PO2 levels in the tibialis anterior (TA) muscle were measured using the phosphorescence quenching method during the transition from rest to electrically induced muscle contractions. ΔPO2 was calculated as PO2mv minus PO2is.

Results: At rest, there were no differences in PO2mv, PO2is, and ΔPO2 between the sham and CLP groups. The PO2mv and PO2is decreased during the transition from rest to contractions in both groups. During muscle contractions, the PO2mv and ΔPO2 were significantly lower in the CLP group than in the sham group (PO2mv, 7.79 ± 5.03 vs. 4.43 ± 2.23; ΔPO2, 5.50 ± 4.46 vs. 1.82 ± 2.66 mmHg; sham vs. CLP; P < 0.05). The ΔPO2 of the rats in the sham group remained unchanged; however, it markedly decreased during muscle contractions in the CLP group.

Conclusion: Sepsis reduced transcapillary PO2 gradients in contracting skeletal muscles.

查看原文本刊更多论文

脓毒症模型大鼠收缩肌的经毛细血管PO2梯度。

目的：本研究旨在评估盲肠结扎和穿刺（CLP）诱导脓毒症对收缩骨骼肌经毛细血管PO2梯度（ΔPO2）的影响。方法：雄性Wistar大鼠（n = 16、10周龄）随机分为假手术组和CLP组。术后7天，采用磷光猝灭法测定静息到电致肌肉收缩过渡期间胫骨前肌（TA）微血管（PO2mv）和间质（PO2is） PO2水平。ΔPO2计算为PO2mv - PO2is。结果：静息时，假手术组与CLP组PO2mv、PO2is、ΔPO2均无差异。两组PO2mv和PO2is均在静息期到宫缩期的过渡中下降。在肌肉收缩过程中，CLP组PO2mv和ΔPO2明显低于假手术组(PO2mv, 7.79±5.03 vs 4.43±2.23；ΔPO2, 5.50±4.46 vs. 1.82±2.66 mmHg；sham vs. CLP；P < 0.05)。假手术组大鼠ΔPO2不变；然而，CLP组在肌肉收缩过程中明显降低。结论：脓毒症降低了收缩骨骼肌的经毛细血管PO2梯度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Vascular Research 医学-生理学

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The ''Journal of Vascular Research'' publishes original articles and reviews of scientific excellence in vascular and microvascular biology, physiology and pathophysiology. The scope of the journal covers a broad spectrum of vascular and lymphatic research, including vascular structure, vascular function, haemodynamics, mechanics, cell signalling, intercellular communication, growth and differentiation. JVR''s ''Vascular Update'' series regularly presents state-of-the-art reviews on hot topics in vascular biology. Manuscript processing times are, consistent with stringent review, kept as short as possible due to electronic submission. All articles are published online first, ensuring rapid publication. The ''Journal of Vascular Research'' is the official journal of the European Society for Microcirculation. A biennial prize is awarded to the authors of the best paper published in the journal over the previous two years, thus encouraging young scientists working in the exciting field of vascular biology to publish their findings.