Journal of Vascular Research最新文献_第9页

Preservation of Adrenoceptor and Endothelin Receptor Mediated Vasoconstriction and of Endothelium-Dependent Relaxation after Cold Storage of Explanted Blood Vessels for ex vivo Analyses. 外植血管冷藏后肾上腺素受体和内皮素受体介导的血管收缩和内皮依赖性松弛的保存

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-01-01 Epub Date: 2022-06-21 DOI: 10.1159/000524922

Markus Hoenicka, Marius Sabau, Andreas Liebold, Hans-Stefan Hofmann, Michael Ried

{"title":"Preservation of Adrenoceptor and Endothelin Receptor Mediated Vasoconstriction and of Endothelium-Dependent Relaxation after Cold Storage of Explanted Blood Vessels for ex vivo Analyses.","authors":"Markus Hoenicka, Marius Sabau, Andreas Liebold, Hans-Stefan Hofmann, Michael Ried","doi":"10.1159/000524922","DOIUrl":"https://doi.org/10.1159/000524922","url":null,"abstract":"Introduction: Adrenoceptor and endothelin (ET) receptor-mediated vasoconstriction as well as endothelium-dependent vasodilation of human saphenous veins were compared before and after 20 h of cold storage.Methods: Contractile responses to potassium chloride (KCl), norepinephrine (NE), and ET-1 as well as vasodilator responses to acetylcholine (ACh) were evaluated.Results: Storage in HEPES-supplemented Dulbecco's modified Eagle's medium (HDMEM) diminished KCl induced contractile forces to 71% (p = 0.002) and NE induced contractions to 80% (p = 0.037), in contrast to HEPES-supplemented Krebs-Henseleit solution (HKH) and TiProtec solution. KCl-normalized NE contractions were not affected by storage. NE EC50 values were slightly lower (7.1E-8 vs. 7.5E-8, p = 0.019) after storage in HKH, with no changes after storage in the other solutions. Endothelium-dependent responses to ACh were not affected by storage. ET-1 induced contractions were attenuated after storage in HDMEM (77%, p = 0.002), HKH (75%, p = 0.020), and TiProtec (73%, p = 0.010) with no changes in normalized constrictions. ET-1 EC50 values were not affected by storage.Conclusion: Loss of contractility after storage in HDMEM may reflect the lower content of dextrose. There was no specific attenuation of adrenoceptor, ET-receptor, or ACh receptor mediated signal transduction after storage in any of the media. HKH or TiProtec are equally suitable cold storage solutions for ex vivo measurements.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40150908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perivascular Adipose Tissue Anticontractile Function Is Mediated by Both Endothelial and Neuronal Nitric Oxide Synthase Isoforms. 血管周围脂肪组织抗收缩功能是由内皮和神经元一氧化氮合酶同工型介导的。

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-01-01 Epub Date: 2022-08-10 DOI: 10.1159/000526027

Sophie N Saxton, Sarah B Withers, Anthony M Heagerty

{"title":"Perivascular Adipose Tissue Anticontractile Function Is Mediated by Both Endothelial and Neuronal Nitric Oxide Synthase Isoforms.","authors":"Sophie N Saxton, Sarah B Withers, Anthony M Heagerty","doi":"10.1159/000526027","DOIUrl":"https://doi.org/10.1159/000526027","url":null,"abstract":"Background: The mechanism of the perivascular adipose tissue (PVAT) anticontractile effect is well characterized in rodent visceral vascular beds; however, little is known about the mechanism of PVAT anticontractile function in subcutaneous vessels. In addition, we have previously shown that PVAT anticontractile function is nitric oxide synthase (NOS) dependent but have not investigated the roles of NOS isoforms.Objective: Here, we examined PVAT anticontractile function in the mouse gracilis artery, a subcutaneous fat depot, in lean control and obese mice and investigated the mechanism in comparison to a visceral depot.Method: Using the wire myograph, we generated responses to noradrenaline and electrical field stimulation in the presence of pharmacological tools targeting components of the known PVAT anticontractile mechanism. In addition, we performed ex vivo \"fat transplants\" in the organ bath.Results: The mechanism of PVAT anticontractile function is similar between subcutaneous and visceral PVAT depots. Both endothelial and neuronal NOS isoforms mediated the PVAT anticontractile effect. Loss of PVAT anticontractile function in obesity is independent of impaired vasoreactivity, and function can be restored in visceral PVAT by NOS activation.Conclusions: Targeting NOS isoforms may be useful in restoring PVAT anticontractile function in obesity, ameliorating increased vascular tone, and disease.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40600518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Quantification of Lipid Area within Thermogenic Mouse Perivascular Adipose Tissue Using Standardized Image Analysis in FIJI. 在 FIJI 中使用标准化图像分析量化热源小鼠血管周围脂肪组织内的脂质面积。

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-01-01 Epub Date: 2021-11-04 DOI: 10.1159/000517178

Benjamin W Tero, Bethany Fortier, Ashley N Soucy, Ginger Paquette, Lucy Liaw

{"title":"Quantification of Lipid Area within Thermogenic Mouse Perivascular Adipose Tissue Using Standardized Image Analysis in FIJI.","authors":"Benjamin W Tero, Bethany Fortier, Ashley N Soucy, Ginger Paquette, Lucy Liaw","doi":"10.1159/000517178","DOIUrl":"10.1159/000517178","url":null,"abstract":"Quantification of adipocyte size and number is routinely performed for white adipose tissues using existing image analysis software. However, thermogenic adipose tissue has multilocular adipocytes, making it difficult to distinguish adipocyte cell borders and to analyze lipid proportion using existing methods. We developed a simple, standardized method to quantify lipid content of mouse thermogenic adipose tissue. This method, using FIJI analysis of hematoxylin/eosin stained sections, was highly objective and highly reproducible, with ∼99% inter-rater reliability. The method was compared to direct lipid staining of adipose tissue, with comparable results. We used our method to analyze perivascular adipose tissue (PVAT) from C57BL/6 mice on a normal chow diet, compared to calorie restriction or a high fat diet, where lipid storage phenotypes are known. Results indicate that lipid content can be estimated within mouse PVAT in a quantitative and reproducible manner, and shows correlation with previously studied molecular and physiological measures.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766879/pdf/nihms-1726193.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10514590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inflammation and Microcalcification: A Never-Ending Vicious Cycle in Atherosclerosis? 炎症和微钙化:动脉粥样硬化永无止境的恶性循环?

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-01-01 Epub Date: 2022-01-17 DOI: 10.1159/000521161

Lynn Kawtharany, Laurence Bessueille, Hawraa Issa, Eva Hamade, Kazem Zibara, David Magne

{"title":"Inflammation and Microcalcification: A Never-Ending Vicious Cycle in Atherosclerosis?","authors":"Lynn Kawtharany, Laurence Bessueille, Hawraa Issa, Eva Hamade, Kazem Zibara, David Magne","doi":"10.1159/000521161","DOIUrl":"https://doi.org/10.1159/000521161","url":null,"abstract":"Inflammatory cells and cytokines are known for long to worsen the development of atherosclerotic plaques in mice, and intense efforts are today devoted to develop anti-inflammatory therapeutic strategies to slow down plaque development. Increasing data indicate that plaque inflammation is intimately associated with microcalcifications, which exert harmful effects eventually culminating with plaque rupture. In this review article, we will first introduce microcalcification location, detection, and effects in atherosclerotic plaques. Then, we will present the numerous data suggesting that inflammatory cells and molecules are responsible for the formation of microcalcifications and the articles showing that microcalcifications stimulate macrophages and smooth muscle cells to produce more pro-inflammatory cytokines. Finally, we will discuss the possibility that microcalcifications might stimulate smooth muscle cells to produce larger and more stable calcifications to stabilize plaques, to exit the vicious cycle associating inflammation and microcalcification in atherosclerotic plaques.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39828072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Association between Arteriosclerosis and Uncontrolled Blood Pressure in Patients with Essential Hypertension: Cross-Sectional Observational Study Results of the BEST Study. 高血压患者动脉硬化与未控制血压之间的关系:BEST研究的横断面观察研究结果

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-01-01 Epub Date: 2022-03-24 DOI: 10.1159/000520744

Huan Liu, Wei Huang, Jinbo Liu, Na Zhao, Hongyu Wang

{"title":"Association between Arteriosclerosis and Uncontrolled Blood Pressure in Patients with Essential Hypertension: Cross-Sectional Observational Study Results of the BEST Study.","authors":"Huan Liu, Wei Huang, Jinbo Liu, Na Zhao, Hongyu Wang","doi":"10.1159/000520744","DOIUrl":"https://doi.org/10.1159/000520744","url":null,"abstract":"Whether arteriosclerosis can influence the hypertension control remains incompletely understood. We hypothesized that higher arteriosclerosis may be associated with uncontrolled hypertension. Arteriosclerosis was assessed by carotid femoral artery pulse wave velocity (CF-PWV) and uncontrolled hypertension (systolic blood pressure [BP] ≥130 mm Hg or diastolic BP ≥80 mm Hg). The multivariable-adjusted logistic regression was used for analysis. A total of 1,428 patients with essential hypertension (mean age 68 years, 49.6% male) were enrolled into the study from 2010 to 2017. The BP was uncontrolled in 50.7% of the participants and the mean level of CF-PWV was 12.3 m/s. All the cardiovascular risk factors were worse and CF-PWV was higher in patients with uncontrolled hypertension (all p < 0.05). Multivariable-adjusted logistic regression analysis showed that CF-PWV as a continuous variable (odd ratio [OR] 1.093, 95% confidence interval [CI] 1.046-1.142) was independently associated with uncontrolled hypertension, after male (OR 1.511) and total cholesterol (OR 1.167), followed by body mass index (OR 1.092), fasting plasma glucose (OR 1.075), and creatinine (OR 1.010) (all p < 0.05). As a binary variable, CF-PWV >12 m/s was also independently associated with uncontrolled hypertension (OR 1.690, 95% CI 1.320-2.164, p < 0.05). Arteriosclerosis is independently associated with uncontrolled BP in patients with hypertension.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40319827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Contents, Vol. 59, 2022. 目录，第59卷，2022年。

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-01-01 DOI: 10.1159/000528269

引用次数: 0

Morphologic Mapping of the Sublingual Microcirculation in Healthy Volunteers. 健康志愿者舌下微循环的形态学定位。

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-01-01 Epub Date: 2022-03-21 DOI: 10.1159/000522394

Göksel Güven, Zühre Uz, Matthias P Hilty, Burak Bilecenoğlu, Șakir Akin, Yasin Ince, Can Ince

{"title":"Morphologic Mapping of the Sublingual Microcirculation in Healthy Volunteers.","authors":"Göksel Güven, Zühre Uz, Matthias P Hilty, Burak Bilecenoğlu, Șakir Akin, Yasin Ince, Can Ince","doi":"10.1159/000522394","DOIUrl":"https://doi.org/10.1159/000522394","url":null,"abstract":"Purpose: Monitoring the sublingual and oral microcirculation (SM-OM) using hand-held vital microscopes (HVMs) has provided valuable insight into the (patho)physiology of diseases. However, the microvascular anatomy in a healthy population has not been adequately described yet.Methods: Incident dark field-based HVM imaging was used to visualize the SM-OM. First, the SM was divided into four different fields; Field-a (between incisors-lingua), Field-b (between the canine-first premolar-lingua), Field-c (between the first-second premolar-lingua), Field-d (between the second molar-wisdom teeth-lingua). Second, we investigated the buccal area, lower and upper lip. Total/functional vessel density (TVD/FCD), focus depth (FD), small vessel mean diameters (SVMDs), and capillary tortuosity score (CTS) were compared between the areas.Results: Fifteen volunteers with a mean age of 29 ± 6 years were enrolled. No statistical difference was found between the sublingual fields in terms of TVD (p = 0.30), FCD (p = 0.38), and FD (p = 0.09). SVMD was similar in Field-a, Field-b, and Field-c (p = 0.20-0.30), and larger in Field-d (p < 0.01, p = 0.015). The CTS of the buccal area was higher than in the lips.Conclusion: The sublingual area has a homogenous distribution in TVD, FCD, FD, and SVMD. This study can be a description of the normal microvascular anatomy for future researches regarding microcirculatory assessment.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40311747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

CIDEC: A Potential Factor in Diabetic Vascular Inflammation. 糖尿病血管炎症的一个潜在因素。

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-01-01 Epub Date: 2022-02-04 DOI: 10.1159/000520685

Fang-Qiang Song, Hui-Min Zhou, Wei-Xuan Ma, Yu-Lin Li, Bo-Ang Hu, Yuan-Yuan Shang, Zhi-Hao Wang, Ming Zhong, Wei Zhang, Yun Ti

引用次数: 2

Apolipoprotein A-I Inhibits Transendothelial Transport of Apolipoprotein B-Carrying Lipoproteins and Enhances Its Associated High-Density Lipoprotein Formation. 载脂蛋白 A-I 可抑制携带载脂蛋白 B 的脂蛋白的跨内皮转运，并增强与之相关的高密度脂蛋白的形成。

IF 1.8 4区医学

Journal of Vascular Research Pub Date : 2022-01-01 Epub Date: 2022-06-27 DOI: 10.1159/000525259

Zhongmao Guo, Ningya Zhang, Hong Yang

{"title":"Apolipoprotein A-I Inhibits Transendothelial Transport of Apolipoprotein B-Carrying Lipoproteins and Enhances Its Associated High-Density Lipoprotein Formation.","authors":"Zhongmao Guo, Ningya Zhang, Hong Yang","doi":"10.1159/000525259","DOIUrl":"10.1159/000525259","url":null,"abstract":"Caveola-located scavenger receptor type B class I (SR-BI) and activin receptor-like kinase-1 (ALK1) are involved in transendothelial transport of apolipoprotein B-carrying lipoproteins (apoB-LPs). Transport of apoB-LPs though mouse aortic endothelial cells (MAECs) is associated with apoE-carrying high-density lipoprotein (HDL)-like particle formation and apoAI induces raft-located proteins to shift to non-raft membranes by upregulation of ATP-binding cassette transporter A1 (ABCA1). To investigate apoAI's effect on transendothelial transport of apoB-LPs, MAECs and human coronary artery endothelial cells (HCAECs) were treated with apoB-LPs ± apoAI. Our data demonstrated that apoAI neither altered SR-BI and ALK1 expression nor affected apoB-LP binding to MAECs. ApoAI inhibited MAEC uptake, transcellular transport, and intracellular accumulation of apoB-LPs and accelerated their resecretion in MAECs. ApoAI enhanced transendothelial apoB-LP transport-associated HDL-like particle formation, upregulated ABCA1 expression, shifted SR-BI and ALK1 to the non-raft membrane in MAECs, inhibited transcellular transport of apoB-LPs, and enhanced associated HDL-like particle formation in HCAECs. ABCA1 knockdown attenuated apoAI-induced membrane SR-BI and ALK1 relocation and diminished apoAI's effect on transendothelial apoB-LP transport and HDL-like particle formation in MAECs. This suggests that upregulation of ABCA1 expression is a mechanism, whereby apoAI provokes caveola-located receptor relocation, inhibits transendothelial apoB-LP transport, and promotes associated HDL-like particle formation.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847247/pdf/nihms-1817007.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9756057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Pilot Study Examining the Effects of Ischemic Conditioning on Walking Capacity and Lower Extremity Muscle Performance in Patients with Claudication. 缺血性调节对跛行患者行走能力和下肢肌肉功能影响的初步研究。

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-01-01 Epub Date: 2022-09-06 DOI: 10.1159/000525166

Rory J Loo, Max V Wohlauer, Sergey S Tarima, Elizabeth Weseman, Jennifer N Nguyen, Neel A Mansukhani, Matthew J Durand

{"title":"A Pilot Study Examining the Effects of Ischemic Conditioning on Walking Capacity and Lower Extremity Muscle Performance in Patients with Claudication.","authors":"Rory J Loo, Max V Wohlauer, Sergey S Tarima, Elizabeth Weseman, Jennifer N Nguyen, Neel A Mansukhani, Matthew J Durand","doi":"10.1159/000525166","DOIUrl":"https://doi.org/10.1159/000525166","url":null,"abstract":"Introduction: This study investigated whether a novel therapy called ischemic conditioning (IC) improves walking capacity and lower extremity muscle performance in patients with peripheral vascular disease who experience intermittent claudication.Methods: Forty-three patients with claudication were enrolled and received either IC or IC Sham for 2 weeks in this randomized, controlled, double-blinded, prospective study. IC sessions involved five cycles of alternating 5-min inflations of a blood pressure cuff to 225 mm Hg (25 mm Hg for IC Sham) and 5-min deflations, around the thigh of the affected lower extremity.Results: There was no difference in the change in claudication onset time (Δ = 114 ± 212 s IC vs. 104 ± 173 s IC Sham; p = 0.67) or peak walking time (Δ = 42 ± 139 s IC vs. 12 ± 148 s IC Sham; p = 0.35) between the IC and IC Sham groups. At the level of the knee, participants in the IC group performed more work (Δ = 3,029 ± 4,999 J IC vs. 345 ± 2,863 J IC Sham; p = 0.03) and displayed a greater time to muscle fatigue (Δ = 147 ± 221 s IC vs. -27 ± 236 s IC Sham; p = 0.01).Discussion/conclusion: In patients with claudication, IC improved total work performed and time to fatigue at the knee but did not change walking parameters.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40353048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0