Journal of Tropical Medicine最新文献

{"title":"MicroRNA-1 Inhibits the Growth of Breast Cancer Cells MDA-MB-231 and MCF-7 Treated with Hydatid Cyst Fluid","authors":"Hadis Jafari, Mahmoud Mahami-Oskouei, Adel Spotin, Behzad Baradaran, Dariush Shanehbandi, Amir Baghbanzadeh, Zahra Alizadeh","doi":"10.1155/2024/7474039","DOIUrl":"https://doi.org/10.1155/2024/7474039","url":null,"abstract":"Antigens in hydatid cyst fluid (HCF) have been discovered to bear a significant resemblance to antigens present in cancer cells. MicroRNA-1 (miR-1) is a well-known member of the tumor inhibitor miRNA family and has been shown to have pro-apoptotic and tumor-inhibitory functions. This study aimed to evaluate the ability of HCF to prevent breast cancer and to explore the underlying mechanisms that affect cancer cells. For this study, MDA-MB-231 and MCF-7 breast cancer cells were cultured and divided into two groups: one group received HCF treatment and the other group was untreated and served as the control group. The cytotoxicity and cell viability of various HCF concentrations on breast cancer cells were evaluated using the MTT assay. In addition, the expression level of miR-1 in HCF-treated and untreated breast cancer cells was analyzed using qRT-PCR. The study found that HCF treatment reduced the growth of MDA-MB-231 and MCF-7 breast cancer cells, indicating that it was cytotoxic to the cells. Specifically, the IC50 concentration of HCF after 24 hours of treatment was 7.32 <i>µ</i>g/mL for MDA-MB-231 cells and 13.63 <i>µ</i>g/mL for MCF-7 cells. In addition, qRT-PCR analysis revealed that the expression level of miR-1 was significantly increased in HCF-treated MDA-MB-231 (<span><svg height=\"8.8423pt\" style=\"vertical-align:-0.2064009pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 19.289 8.8423\" width=\"19.289pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,11.658,0)\"></path></g></svg><span></span><span><svg height=\"8.8423pt\" style=\"vertical-align:-0.2064009pt\" version=\"1.1\" viewbox=\"22.8711838 -8.6359 34.445 8.8423\" width=\"34.445pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,22.921,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,29.161,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,32.125,0)\"><use xlink:href=\"#g113-49\"></use></g><g transform=\"matrix(.013,0,0,-0.013,38.365,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,44.605,0)\"><use xlink:href=\"#g113-49\"></use></g><g transform=\"matrix(.013,0,0,-0.013,50.845,0)\"></path></g></svg>)</span></span> and MCF-7 (<span><svg height=\"8.8423pt\" style=\"vertical-align:-0.2064009pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 19.289 8.8423\" width=\"19.289pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"><use xlink:href=\"#g113-81\"></use></g><g transform=\"matrix(.013,0,0,-0.013,11.658,0)\"><use xlink:href=\"#g117-34\"></use></g></svg><span></span><span><svg height=\"8.8423pt\" style=\"vertical-align:-0.2064009pt\" version=\"1.1\" viewbox=\"22.8711838 -8.6359 34.445 8.8423\" width=\"34.445pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,22.921,0)\"><use xlink:href=\"#g113-49\"></use><","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"38 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140106911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and Antioxidant Efficacies of Secondary Metabolites from the Roots of Cyphostemma adenocaule: A Combined In Vitro and In Silico Study","authors":"Hadush Gebrehiwot, Yadessa Melaku, Muhdin Aliye, Urgessa Ensermu, Aman Dekebo, Milkyas Endale, Daniel Rentsch, Mo Hunsen","doi":"10.1155/2024/1679695","DOIUrl":"https://doi.org/10.1155/2024/1679695","url":null,"abstract":"<i>Cyphostemma adenocaule</i> is a therapeutic plant traditionally used to treat rabies, snake bite, diarrhea, and wound healing. To address the bioactive compounds exhibiting these activities, we performed a comprehensive study on the roots of the plant. Thus, the present study aims to inspect the <i>in vitro</i> antioxidant and antibacterial efficacies of compounds isolated from the combined dichloromethane : methanol (1 : 1) and methanol extracts of <i>C. adenocaule</i> along with the <i>in silico</i> study of their interaction with selected protein targets. The silica gel column chromatography technique was used for the isolation of compounds, and the antibacterial and antioxidant activities were evaluated using agar disc diffusion and DPPH radical scavenging assays, respectively. Furthermore, <i>in silico</i> molecular docking screening, pharmacokinetics, and toxicity protocols of the compound isolates were performed to offer the potential applications of the compounds in developing novel medications. A BIOVIA Discovery Studio in combination with AutoDock Vina 4.2 software, SwissADME, and ProTox-II prediction web tools were used to generate the molecular docking, pharmacokinetics, and toxicity profiles, respectively. Notably, the chromatographic separation of the combined extracts yielded six known compounds, namely, <i>β-</i>sitosterol (<b>1</b>), 3-hydroxyisoagatholactone (<b>2</b>), <i>ε</i>-viniferin (<b>3</b>), myricetin (<b>4</b>), tricuspidatol A (<b>5</b>), and parthenocissin A (<b>6</b>). The <i>in vitro</i> antibacterial activities revealed the highest inhibition zone by tricuspidatol A (<b>5</b>) (16.67 ± 0.47), showcasing its potent activity against <i>S. aureus</i> at 2 mg/mL, compared to ciprofloxacin (21.50 ± 0.41). <i>ε</i>-Viniferin (<b>3</b>) (IC₅₀: 0.32 <i>μ</i>g/mL) exhibited greater antioxidant activity than the others and displayed promising results compared to ascorbic acid (0.075 <i>μ</i>g/mL). The molecular docking study revealed the highest binding affinity by <i>ε-</i>viniferin (<b>3</b>) (−9.9 kcal/mol) against topoisomerase II <i>α.</i> 3-Hydroxyisoagatholactone (<b>2</b>) and <i>ε</i>-viniferin (<b>3</b>) fulfilled Lipinski’s rule with no violation, and the organ toxicity predictions revealed that all the compounds showed no cytotoxicity and hepatotoxicity effects. Thus, this study’s combined <i>in vitro</i> and <i>in silico</i> outcomes suggest the potential use of the isolated compounds in drug discovery and support the traditional relevance of <i>C. adenocaule</i>.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"34 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Malaria Burden: A South African Perspective","authors":"Marissa Balmith, Charlise Basson, Sarel J. Brand","doi":"10.1155/2024/6619010","DOIUrl":"https://doi.org/10.1155/2024/6619010","url":null,"abstract":"Malaria is a deadly disease caused by protozoan pathogens of the <i>Plasmodium</i> parasite. Transmission to humans occurs through the bite of an infected female <i>Anopheles</i> mosquito. According to the World Health Organization (WHO), an estimated 247 million cases of malaria were recorded worldwide in 2021, with approximately 619 000 malaria deaths. The initial signs of malaria can be mild and challenging to diagnose due to the signs and symptoms being similar to those of other illnesses. The malaria burden remains largely concentrated in the WHO sub-Saharan African region and has been recognised as a significant contributor to morbidity and mortality. This review aims to contribute to the existing knowledge on malaria in South Africa, a region within sub-Saharan Africa, focusing on the epidemiology and life cycle of the malaria parasite as well as diagnostic approaches for detecting malaria. In addition, nonpharmacological and pharmacological interventions for treating and preventing malaria infections will also be discussed herein. While there has been a significant reduction in the global burden of this disease, malaria remains a public health issue in South Africa. As such, the implementation of effective preventative measures and strategies, early diagnosis, and appropriate treatment regimens are crucial to reducing the malaria burden in South Africa.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"44 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139955414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic Review and Meta-Analysis of Congenital Toxoplasmosis Diagnosis: Advances and Challenges","authors":"Priscila Silva Franco, Ana Carolina Morais Oliveira Scussel, Rafaela José Silva, Thadia Evelyn Araújo, Henrique Tomaz Gonzaga, Camila Ferreira Marcon, Joaquim Pedro Brito-de-Sousa, Angélica Lemos Debs Diniz, Marina Carvalho Paschoini, Bellisa Freitas Barbosa, Olindo Assis Martins-Filho, José Roberto Mineo, Eloisa Amália Vieira Ferro, Angelica Oliveira Gomes","doi":"10.1155/2024/1514178","DOIUrl":"https://doi.org/10.1155/2024/1514178","url":null,"abstract":"<i>Objective</i>. To understand how congenital toxoplasmosis (CT) diagnosis has evolved over the years, we performed a systematic review and meta-analysis to summarize the kind of analysis that has been employed for CT diagnosis. <i>Methods</i>. PubMed and Lilacs databases were used in order to access the kind of analysis that has been employed for CT diagnosis in several samples. Our search combined the following combining terms: “congenital toxoplasmosis” or “gestational toxoplasmosis” and “diagnosis” and “blood,” “serum,” “amniotic fluid,” “placenta,” or “colostrum.” We extracted data on true positive, true negative, false positive, and false negative to generate pooled sensitivity, specificity, and diagnostic odds ratio (DOR). Random-effects models using MetaDTA were used for analysis. <i>Results</i>. Sixty-five articles were included in the study aiming for comparisons (75.4%), diagnosis performance (52.3%), diagnosis improvement (32.3%), or to distinguish acute/chronic infection phases (36.9%). Amniotic fluid (AF) and placenta were used in 36.9% and 10.8% of articles, respectively, targeting parasites and/or <i>T. gondii</i> DNA. Blood was used in 86% of articles for enzymatic assays. Colostrum was used in one article to search for antibodies. In meta-analysis, PCR in AF showed the best performance for CT diagnosis based on the highest summary sensitivity (85.1%) and specificity (99.7%) added to lower magnitude heterogeneity. <i>Conclusion</i>. Most of the assays being researched to diagnose CT are basically the same traditional approaches available for clinical purposes. The range in diagnostic performance and the challenges imposed by CT diagnosis indicate the need to better explore pregnancy samples in search of new possibilities for diagnostic tools. Exploring immunological markers and using bioinformatics tools and <i>T. gondii</i> recombinant antigens should address the research needed for a new generation of diagnostic tools to face these challenges.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"1 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139922867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling Dengue Dynamics: In-Depth Epidemiological and Entomological Analyses in Bengaluru, India","authors":"Vani H. C., Sujit Nath N., Jaiswal M. K., Tiwari R. P., Bharathi P., Asmita B., Shankar G., Jithen C., Pallavi V. D., Srinivas V., Ashok M., Mahamood Shariff, Renuka S., Shrinivasa B. M.","doi":"10.1155/2024/7247263","DOIUrl":"https://doi.org/10.1155/2024/7247263","url":null,"abstract":"In view of the increased number of detected dengue cases in Bengaluru, a request for situation analysis was received from local health authorities in the selected area. The study included epidemiological and entomological assessments to understand the same. The immature forms collected were allowed to emerge, pooled, and processed for vector incrimination. In the surveyed population (347), 20 (5.8%) reported fever cases and 12 (3.5%) were confirmed as dengue cases among the 102 families. Stegomyia indices were high. Vector incrimination studies revealed pools positive for the presence of dengue virus in flower pots, fridge trays, plastic barrels, and rubber tires habitats. This study highlights the increased risk of dengue fever incidence in communities wherepoor intra and peri-domestic sanitation practices are prevailing and recommendsregular entomological surveillance of denguevirus in its vector population..","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"43 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139771150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breeding Habitat Preference of the Dengue Vector Mosquitoes Aedes aegypti and Aedes albopictus from Urban, Semiurban, and Rural Areas in Kurunegala District, Sri Lanka","authors":"J. M. Manel K. Herath, W. A. Priyanka P. De Silva, Thilini C. Weeraratne, S. H. P. Parakrama Karunaratne","doi":"10.1155/2024/4123543","DOIUrl":"https://doi.org/10.1155/2024/4123543","url":null,"abstract":"Elimination of vector mosquito larvae and their breeding environments is an effective strategy in dengue disease control. Present study examined larval density and water quality in breeding habitats and container preference of dengue vectors <i>Ae. aegypti</i> and <i>Ae. albopictus</i>. Larval surveys were conducted monthly in urban, semiurban, and rural sites in Kurunegala, Sri Lanka, from January 2019 to December 2021. Larval densities were recorded under the following three categories: type of container (16 types), type of material (6 types), and location (indoor/outdoor). Breeding preference ratios (BPRs) were calculated using Index of Available Containers and the Index of Contribution to Breeding Sites. Out of 19,234 wet containers examined, larval stages were found in 1,043 habitats. <i>Ae. albopictus</i> larvae were in all three areas whereas <i>Ae. aegypti</i> larvae were restricted to urban areas. Highest number of wet containers and highest positivity were reported from urban followed by semiurban. In general, discarded nondegradable items were the most frequent and mostly positive breeding sites. For <i>Ae</i>. <i>aegypti</i>, the most preferred breeding sites were gutters and concrete slabs. <i>Ae. albopictus</i> mostly preferred concrete slabs in urban areas and tyres in semiurban and rural areas. Material types such as rubber and concrete were mostly preferred by <i>Ae. aegypti</i> whereas ceramic was preferred by <i>Ae. albopictus</i>. Although plastic was the most available material type in all study sites, preference to plastic was low except for urban <i>Ae. albopictus</i>. Both species preferred urban indoor breeding habitats although outdoor breeding was preferred by <i>Ae. albopictus</i> in rural areas. Larval densities of <i>Ae. aegypti</i> and semiurban <i>Ae. albopictus</i> significantly correlated with the BPR of the container type and material type. Dengue vector larvae were found in a 6.7–9.4 pH range. Total dissolved solids and alkalinity positively correlated with preference. Information generated can be successfully used in waste management and public education for effective vector control.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"159 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139580761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Factories on Adherence to COVID-19 Standard Operating Procedures: A Case Study of Wakiso, Mukono, Buikwe, and Jinja Districts, Uganda","authors":"Joseph M. Kungu, Edity Namyalo, Angella Musewa, Sarah Nitumusiima, Phiona Keije, Catherine Nakakooza, Osborn Oyirwoth","doi":"10.1155/2024/6670510","DOIUrl":"https://doi.org/10.1155/2024/6670510","url":null,"abstract":"&lt;i&gt;Background&lt;/i&gt;. Coronavirus disease (COVID-19) is an infectious disease caused by the SARS-CoV-2 virus. Uganda confirmed the first case of COVID-19 on 21st March, 2020, which led to the first total lockdown in the country. During the lockdown, some factories remained operational; hence, there is a need for a study aimed at assessing the level of adherence to COVID-19 standard operating procedures (SOPs) in factories as a mitigator for the pandemic. &lt;i&gt;Methods&lt;/i&gt;. A cross-sectional study to assess compliance of factories to COVID-19 SOPs was conducted in Wakiso, Mukono, Buikwe, and Jinja districts during the month of September, 2021. This involved visitation of factories and collection of data using the KoboCollect tool by interviewing general managers as well as human resource managers of the factories. A total of 39 factories were included in the study and were categorized into four major groups; food and beverages (15), plastics (5), construction (8), and others (11). Data analysis was done using STATA version 14.2. &lt;i&gt;Results&lt;/i&gt;. Overall adherence to COVID-19 SOPs by the factories was 64.1% (95% CI = 49.1–79.1). Communication and training of employees (79.5%; 95% CI = 66.8–92.2), wearing personal protective equipment (PPE), and respiratory protection (79.5%; 95% CI = 66.8–92.2) as well as enhanced cleaning and disinfection of surfaces (74.4%; 95% CI = 60.6–88.1) were the most implemented SOPs in the factories. Implementation of the SOPs was the highest in Mukono district (88.9%; 95% CI = 68.4–100) and the least in Wakiso district (40.0%; 95% CI = 9.6–70.4). The COVID-19 SOPs were followed mostly in construction material factories (87.5%; 95% CI = 64.6–100) and least in food and beverage factories (40%; 95% CI = 15.2–64.8). There was no significant difference in the adherence of COVID-19 SOPs among the districts (&lt;svg height=\"11.6412pt\" style=\"vertical-align:-0.04979992pt\" version=\"1.1\" viewbox=\"-0.0498162 -11.5914 15.0284 11.6412\" width=\"15.0284pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,0,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.0091,0,0,-0.0091,9.945,-5.741)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;/svg&gt; = 5.02 and &lt;span&gt;&lt;svg height=\"8.8423pt\" style=\"vertical-align:-0.2064009pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 19.289 8.8423\" width=\"19.289pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,0,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,11.658,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;/svg&gt;&lt;span&gt;&lt;/span&gt;&lt;span&gt;&lt;svg height=\"8.8423pt\" style=\"vertical-align:-0.2064009pt\" version=\"1.1\" viewbox=\"22.8711838 -8.6359 21.918 8.8423\" width=\"21.918pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,22.921,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,29.161,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,32.125,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,38.365,0)\"&gt;&lt;/p","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"1 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139474988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Deltamethrin-Laced Attractive Toxic Sugar Bait to Control Aedes aegypti (Linnaeus) Population","authors":"Sarita Kumar, Aarti Sharma, Roopa Rani Samal, Vaishali Verma, Ravinder Kumar Sagar, Shri Pati Singh, Kamaraju Raghavendra","doi":"10.1155/2024/6966205","DOIUrl":"https://doi.org/10.1155/2024/6966205","url":null,"abstract":"<i>Background</i>. The attractive toxic sugar bait (ATSB) is a promising strategy for controlling mosquitoes at the adult stage. The strategy is based on the use of a combination of fruit juice, sugar, and a toxin in order to attract and kill the adult mosquitoes. The selection of the components and optimization of their concentrations is significant for the formulation of an effective ATSB. <i>Methods</i>. The present study formulated nine ATSBs and evaluated their efficacy against two laboratory strains (AND-<i>Aedes aegypti</i> and AND-<i>Aedes aegypti-</i>DL10) and two wildcaught colonized strains of <i>Aedes aegypti</i> (GVD-Delhi and SHD-Delhi). Initially, nine attractive sugar baits (ASBs) were prepared using a mixture of 100% fermented guava juice (attractant) with 10% sucrose solution (w/v) in 1 : 1 ratio. ATSBs were formulated by mixing each ASB with different concentrations of deltamethrin in the ratio of 9 : 1 to obtain final deltamethrin concentration of 0.003125–0.8 mg/10 mL ATSB. Cage bioassays were conducted with 50 mosquitoes for 24 h in order to evaluate the efficacy of each ATSB against the four strains of <i>Ae. aegypti</i>. The data were statistically analyzed using PASW software 19.0 program and 2-way ANOVA. <i>Results</i>. The ATSB formulations registered 8.33–97.44% mortality against AND-<i>Aedes aegypti</i> and 5.15–96.91% mortality against AND-<i>Aedes aegypti</i>-DL10 strains of <i>Ae. aegypti</i>, while GVD-Delhi strain registered 2.04–95.83% mortality and SHD-Delhi strain showed 5.10–97.96% mortality. The administration of 0.8 mg of deltamethrin within 10 mL of attractive toxic sugar bait (ATSB) has led to the maximum mortality rate in adult mosquitoes. <i>Conclusions</i>. The ATSBs formulated with guava juice-ASB and deltamethrin (9 : 1) showed toxin dose-dependent toxicity by all the four strains of <i>Ae. aegypti.</i> Most effective dosage was found as 0.8 mg deltamethrin/10 mL ATSB which imparted 96% to 98% mortality in adult mosquitoes. The investigations demonstrated the efficacy of deltamethrin-laced ATSB formulations against <i>Ae. aegypti</i> and highlighted the need for conduct of structured field trials and investigating the impact on disease vectors and nontarget organisms.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"36 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139374244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Diagnostic Performance of Microscopy, SD-Bioline Rapid Diagnostic Test, and Polymerase Chain Reaction in the Detection of Malaria Infection among Pregnant Women at Delivery in Kumba Health District Area in the Southwest Region of Cameroon","authors":"Bekindaka Ngemani Obase, Esemu Livo Forgu, Awanakam Honore Awanakam, Zeukeng Francis, Agnenga Balonga Annie, Nchankou Loic, Jude Daiga Bigoga, Rose Leke, Dickson Shey Nsagha","doi":"10.1155/2023/2056524","DOIUrl":"https://doi.org/10.1155/2023/2056524","url":null,"abstract":"<i>Introduction</i>. Malaria during pregnancy is a major cause of morbidity and mortality in sub-Saharan Africa. Microcopy and rapid diagnostic test (RDT) recommended by the World Health Organization for clinical diagnosis have poor sensitivity to detect individuals with very low levels of parasitemia. Previous studies have shown that malaria in pregnancy is associated with mastitis and excessive uterine blood loss during delivery. However, information evaluating the performance of these tools in detecting malaria in pregnancy at the national level is limited. This study therefore evaluates the performance of microscopy, RDT, and nested polymerase chain reaction (nPCR) in the detection of pregnancy-associated malaria at delivery. <i>Methods</i>. A total of 227 participants constituting of 201 pregnant women without and 26 with HIV were recruited from five health facilities within the Kumba health district area. Mother venous and cord blood were collected at delivery to test for malaria using the thick-film microscopy, SD-bioline RDT, and 18SrRNA-nested PCR. <i>Results</i>. The percentage of malaria-positive cases detected by thick-film microcopy (TFM), RDT, and PCR in pregnant women with and without HIV was 7.69%, 53.85%, and 50% and 3.48%, 23.38%, and 49.25%, respectively. Plasmodium falciparum was detected in 1.99% cord blood samples of women without HIV by PCR. The positivity rate in at least two of the test methods (composite positive) was 42.31% for women with and 19.90% for women without HIV. The sensitivity of TFM and RDT when using PCR as a reference was 7.21% and 49.00%, respectively, in all samples. The specificity was 99.14% and 90.55% with kappa values of 0.064 and 0.461, respectively. When using the composite test as a reference, the sensitivity of TFM, RDT, and nPCR was 15.69%, 94.12%, and 100%, respectively. Specificity was 99.43%, 93.18%, and 65.34% with kappa values of 0.213, 0.821, and 0.458, respectively. <i>Conclusion</i>. This study shows that PCR is more sensitive in the detection of malaria parasite followed by SD-bioline RDT kit. However, in resource-limited settings where access to molecular diagnosis of malaria is a problem, RDT should be considered as the first option to microscopy in the diagnosis of malaria.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"8 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138579731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequency of Gastrointestinal Parasites, Anemia, and Nutritional Status among Children from Different Geographical Regions of Bolivia","authors":"Ceilan Apaza, Washington Cuna, Froilán Brañez, Roberto Passera, Celeste Rodriguez","doi":"10.1155/2023/5020490","DOIUrl":"https://doi.org/10.1155/2023/5020490","url":null,"abstract":"The study aimed to measure the frequency of occurrence of infections with helminths, protozoa, and risk factors of undernutrition and anemia among schoolchildren from the Bolivian highland (altiplano) and lowland (subtropical) rural regions, with a high frequency of gastrointestinal parasite infections. Cross-sectional data were collected from 790 children, 5–13 years old. Microscopic examination of stool using the Ritchie technique, hemoglobin testing using the HemoCue analyzer, and anthropometric measurements were performed. Over 60% and 20% of children were infected with protozoa and helminth parasites, respectively. Infections caused by pathogenic <i>Hymenolepis nana</i> (15.7–5.2%), <i>Ascaris lumbricoides</i> (41.9–28.5%), <i>Giardia lamblia</i> (30.1–11.2%), <i>Entamoeba histolytica</i> (5.7–0.7%), and nonpathogenic <i>Entamoeba coli</i> (48.9–16%), <i>Blastocystis hominis</i> (40.2–28.5%), <i>Iodamoeba butschli</i> (16.1–2.5%), <i>Chilomastix mesnili</i> (19.2–7.3%), and <i>Entamoeba histolytica/dispar</i> (7.4–5.5%) parasites, were more prevalent in the highlands than the lowlands. Single parasitic infections were more prevalent in the lowlands; polyparasitism of light or heavy intensity predominated in the highlands. A strongly increased risk of anemia and a low prevalence of wasting were determined in children in the highlands. A higher risk for stunting was associated with children of older age, and a low burden of intestinal helminths would prevent wasting in children of highlands. Infections with <i>A. lumbricoides</i> and <i>G. lamblia</i> pathogens in older children were not significant covariates for stunting. Environmental, nutritional, and parasitic factors may predispose to anemia in the highlands. A nutritional intervention and parasite control effort will substantially improve children´s health in the highlands.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"94 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138563585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}