Journal of Tissue Engineering最新文献

{"title":"Comparison of extracellular vesicle isolation processes for therapeutic applications.","authors":"Soraya Williams, Maria Fernandez-Rhodes, Alice Law, Ben Peacock, Mark P Lewis, Owen G Davies","doi":"10.1177/20417314231174609","DOIUrl":"10.1177/20417314231174609","url":null,"abstract":"<p><p>While extracellular vesicles (EVs) continue to gain interest for therapeutic applications, their clinical translation is limited by a lack of optimal isolation methods. We sought to determine how universally applied isolation methods impact EV purity and yield. EVs were isolated by ultracentrifugation (UC), polyethylene glycol precipitation, Total Exosome Isolation Reagent, an aqueous two-phase system with and without repeat washes or size exclusion chromatography (SEC). EV-like particles could be detected for all isolation methods but varied in their purity and relative expression of surface markers (Alix, Annexin A2, CD9, CD63 and CD81). Assessments of sample purity were dependent on the specificity of characterisation method applied, with total particle counts and particle to protein (PtP) ratios often not aligning with quantitative measures of tetraspanin surface markers obtained using high-resolution nano-flow cytometry. While SEC resulted in the isolation of fewer particles with a relatively low PtP ratio (1.12 × 10⁷ ± 1.43 × 10⁶ vs highest recorded; ATPS/R 2.01 × 10⁸ ± 1.15 × 10⁹, <i>p</i> ⩽ 0.05), EVs isolated using this method displayed a comparatively high level of tetraspanin positivity (e.g. ExoELISA CD63⁺ particles; 1.36 × 10¹¹ <i>±</i> 1.18 × 10¹⁰ vs ATPS/R 2.58 × 10¹⁰ <i>±</i> 1.92 × 10⁹, <i>p</i> ⩽ 0.001). Results originating from an accompanying survey designed to evaluate pragmatic considerations surrounding method implementation (e.g. scalability and cost) identified that SEC and UC were favoured for overall efficiency. However, reservations were highlighted in the scalability of these methods, which could potentially hinder downstream therapeutic applications. In conclusion, variations in sample purity and yield were evident between isolation methods, while standard non-specific assessments of sample purity did not align with advanced quantitative high-resolution analysis of EV surface markers. Reproducible and specific assessments of EV purity will be critical for informing therapeutic studies.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"14 ","pages":"20417314231174609"},"PeriodicalIF":6.7,"publicationDate":"2023-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10300143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell RNA-sequence analysis of human bone marrow reveals new targets for isolation of skeletal stem cells using spherical nucleic acids.","authors":"Elloise Z Matthews, Stuart Lanham, Kate White, Maria-Eleni Kyriazi, Konstantina Alexaki, Afaf H El-Sagheer, Tom Brown, Antonios G Kanaras, Jonathan J West, Ben D MacArthur, Patrick S Stumpf, Richard Oc Oreffo","doi":"10.1177/20417314231169375","DOIUrl":"10.1177/20417314231169375","url":null,"abstract":"<p><p>There is a wealth of data indicating human bone marrow contains skeletal stem cells (SSC) with the capacity for osteogenic, chondrogenic and adipogenic differentiation. However, current methods to isolate SSCs are restricted by the lack of a defined marker, limiting understanding of SSC fate, immunophenotype, function and clinical application. The current study applied single-cell RNA-sequencing to profile human adult bone marrow populations from 11 donors and identified novel targets for SSC enrichment. Spherical nucleic acids were used to detect these mRNA targets in SSCs. This methodology was able to rapidly isolate potential SSCs found at a frequency of <1 in 1,000,000 in human bone marrow, with the capacity for tri-lineage differentiation in vitro and ectopic bone formation in vivo. The current studies detail the development of a platform to advance SSC enrichment from human bone marrow, offering an invaluable resource for further SSC characterisation, with significant therapeutic impact therein.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"14 ","pages":"20417314231169375"},"PeriodicalIF":8.2,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9504374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preclinical and clinical orthotopic transplantation of decellularized/engineered tracheal scaffolds: A systematic literature review.","authors":"Elena Stocco, Silvia Barbon, Marco Mammana, Giovanni Zambello, Martina Contran, Pier Paolo Parnigotto, Veronica Macchi, Maria Teresa Conconi, Federico Rea, Raffaele De Caro, Andrea Porzionato","doi":"10.1177/20417314231151826","DOIUrl":"10.1177/20417314231151826","url":null,"abstract":"<p><p>Severe tracheal injuries that cannot be managed by mobilization and end-to-end anastomosis represent an unmet clinical need and an urgent challenge to face in surgical practice; within this scenario, decellularized scaffolds (eventually bioengineered) are currently a tempting option among tissue engineered substitutes. The success of a decellularized trachea is expression of a balanced approach in cells removal while preserving the extracellular matrix (ECM) architecture/mechanical properties. Revising the literature, many Authors report about different methods for acellular tracheal ECMs development; however, only few of them verified the devices effectiveness by an orthotopic implant in animal models of disease. To support translational medicine in this field, here we provide a systematic review on studies recurring to decellularized/bioengineered tracheas implantation. After describing the specific methodological aspects, orthotopic implant results are verified. Furtherly, the only three clinical cases of compassionate use of tissue engineered tracheas are reported with a focus on outcomes.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"14 ","pages":"20417314231151826"},"PeriodicalIF":6.7,"publicationDate":"2023-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/80/30/10.1177_20417314231151826.PMC9974632.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9395401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regeneration of articular cartilage defects: Therapeutic strategies and perspectives.","authors":"Xueqiang Guo,&nbsp;Lingling Xi,&nbsp;Mengyuan Yu,&nbsp;Zhenlin Fan,&nbsp;Weiyun Wang,&nbsp;Andong Ju,&nbsp;Zhuo Liang,&nbsp;Guangdong Zhou,&nbsp;Wenjie Ren","doi":"10.1177/20417314231164765","DOIUrl":"https://doi.org/10.1177/20417314231164765","url":null,"abstract":"<p><p>Articular cartilage (AC), a bone-to-bone protective device made of up to 80% water and populated by only one cell type (i.e. chondrocyte), has limited capacity for regeneration and self-repair after being damaged because of its low cell density, alymphatic and avascular nature. Resulting repair of cartilage defects, such as osteoarthritis (OA), is highly challenging in clinical treatment. Fortunately, the development of tissue engineering provides a promising method for growing cells in cartilage regeneration and repair by using hydrogels or the porous scaffolds. In this paper, we review the therapeutic strategies for AC defects, including current treatment methods, engineering/regenerative strategies, recent advances in biomaterials, and present emphasize on the perspectives of gene regulation and therapy of noncoding RNAs (ncRNAs), such as circular RNA (circRNA) and microRNA (miRNA).</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"14 ","pages":"20417314231164765"},"PeriodicalIF":8.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2a/6b/10.1177_20417314231164765.PMC10071204.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9276696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into balancing wound healing and scarless skin repair.","authors":"Shengxi Zhou,&nbsp;Mengbo Xie,&nbsp;Jingjing Su,&nbsp;Bingjie Cai,&nbsp;Jingan Li,&nbsp;Kun Zhang","doi":"10.1177/20417314231185848","DOIUrl":"https://doi.org/10.1177/20417314231185848","url":null,"abstract":"<p><p>Scars caused by skin injuries after burns, wounds, abrasions and operations have serious physical and psychological effects on patients. In recent years, the research of scar free wound repair has been greatly expanded. However, understanding the complex mechanisms of wound healing, in which various cells, cytokines and mechanical force interact, is critical to developing a treatment that can achieve scarless wound healing. Therefore, this paper reviews the types of wounds, the mechanism of scar formation in the healing process, and the current research progress on the dual consideration of wound healing and scar prevention, and some strategies for the treatment of scar free wound repair.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"14 ","pages":"20417314231185848"},"PeriodicalIF":8.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/27/05/10.1177_20417314231185848.PMC10388637.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10302475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibrous hydrogels by electrospinning: Novel platforms for biomedical applications.","authors":"Ji Woo Lee,&nbsp;Kwang Hoon Song","doi":"10.1177/20417314231191881","DOIUrl":"https://doi.org/10.1177/20417314231191881","url":null,"abstract":"<p><p>Hydrogels, hydrophilic and biocompatible polymeric networks, have been used for numerous biomedical applications because they have exhibited abilities to mimic features of extracellular matrix (ECM). In particular, the hydrogels engineered with electrospinning techniques have shown great performances in biomedical applications. Electrospinning techniques are to generate polymeric micro/nanofibers that can mimic geometries of natural ECM by drawing micro/nanofibers from polymer precursors with electrical forces, followed by structural stabilization of them. By exploiting the electrospinning techniques, the fibrous hydrogels have been fabricated and utilized as 2D/3D cell culture platforms, implantable scaffolds, and wound dressings. In addition, some hydrogels that respond to external stimuli have been used to develop biosensors. For comprehensive understanding, this review covers electrospinning processes, hydrogel precursors used for electrospinning, characteristics of fibrous hydrogels and specific biomedical applications of electrospun fibrous hydrogels and highlight their potential to promote use in biomedical applications.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"14 ","pages":"20417314231191881"},"PeriodicalIF":8.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/63/e2/10.1177_20417314231191881.PMC10423451.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10306366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of mechanical loading in bone fracture healing, bone regeneration, and vascularization.","authors":"Qianli Ma,&nbsp;Zahra Miri,&nbsp;Håvard Jostein Haugen,&nbsp;Amirhossein Moghanian,&nbsp;Dagnjia Loca","doi":"10.1177/20417314231172573","DOIUrl":"https://doi.org/10.1177/20417314231172573","url":null,"abstract":"<p><p>In 1892, J.L. Wolff proposed that bone could respond to mechanical and biophysical stimuli as a dynamic organ. This theory presents a unique opportunity for investigations on bone and its potential to aid in tissue repair. Routine activities such as exercise or machinery application can exert mechanical loads on bone. Previous research has demonstrated that mechanical loading can affect the differentiation and development of mesenchymal tissue. However, the extent to which mechanical stimulation can help repair or generate bone tissue and the related mechanisms remain unclear. Four key cell types in bone tissue, including osteoblasts, osteoclasts, bone lining cells, and osteocytes, play critical roles in responding to mechanical stimuli, while other cell lineages such as myocytes, platelets, fibroblasts, endothelial cells, and chondrocytes also exhibit mechanosensitivity. Mechanical loading can regulate the biological functions of bone tissue through the mechanosensor of bone cells intraosseously, making it a potential target for fracture healing and bone regeneration. This review aims to clarify these issues and explain bone remodeling, structure dynamics, and mechano-transduction processes in response to mechanical loading. Loading of different magnitudes, frequencies, and types, such as dynamic versus static loads, are analyzed to determine the effects of mechanical stimulation on bone tissue structure and cellular function. Finally, the importance of vascularization in nutrient supply for bone healing and regeneration was further discussed.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"14 ","pages":"20417314231172573"},"PeriodicalIF":8.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10350396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of nanomaterials in the treatment of intracerebral hemorrhage.","authors":"Xiangyu Zhang,&nbsp;Suliman Khan,&nbsp;Ruixue Wei,&nbsp;Yan Zhang,&nbsp;Yang Liu,&nbsp;Voon Wee Yong,&nbsp;Mengzhou Xue","doi":"10.1177/20417314231157004","DOIUrl":"https://doi.org/10.1177/20417314231157004","url":null,"abstract":"<p><p>Intracerebral hemorrhage (ICH) is a non-traumatic hemorrhage caused by the rupture of blood vessels in the brain parenchyma, with an acute mortality rate of 30%‒40%. Currently, available treatment options that include surgery are not promising, and new approaches are urgently needed. Nanotechnology offers new prospects in ICH because of its unique benefits. In this review, we summarize the applications of various nanomaterials in ICH. Nanomaterials not only enhance the therapeutic effects of drugs as delivery carriers but also contribute to several facets after ICH such as repressing detrimental neuroinflammation, resisting oxidative stress, reducing cell death, and improving functional deficits.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"14 ","pages":"20417314231157004"},"PeriodicalIF":8.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6a/3e/10.1177_20417314231157004.PMC10074624.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9325558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disc regeneration by injectable fucoidan-methacrylated dextran hydrogels through mechanical transduction and macrophage immunomodulation.","authors":"Weifeng Li,&nbsp;Pinghui Zhou,&nbsp;Bomin Yan,&nbsp;Meiyao Qi,&nbsp;Yedan Chen,&nbsp;Lijun Shang,&nbsp;Jianzhong Guan,&nbsp;Li Zhang,&nbsp;Yingji Mao","doi":"10.1177/20417314231180050","DOIUrl":"https://doi.org/10.1177/20417314231180050","url":null,"abstract":"<p><p>Modulating a favorable inflammatory microenvironment that facilitates the recovery of degenerated discs is a key strategy in the treatment of intervertebral disc (IVD) degeneration (IDD). More interestingly, well-mechanized tissue-engineered scaffolds have been proven in recent years to be capable of sensing mechanical transduction to enhance the proliferation and activation of nucleus pulposus cells (NPC) and have demonstrated an increased potential in the treatment and recovery of degenerative discs. Additionally, existing surgical procedures may not be suitable for IDD treatment, warranting the requirement of new regenerative therapies for the restoration of disc structure and function. In this study, a light-sensitive injectable polysaccharide composite hydrogel with excellent mechanical properties was prepared using dextrose methacrylate (DexMA) and fucoidan with inflammation-modulating properties. Through numerous in vivo experiments, it was shown that the co-culture of this composite hydrogel with interleukin-1β-stimulated NPCs was able to promote cell proliferation whilst preventing inflammation. Additionally, activation of the caveolin1-yes-associated protein (CAV1-YAP) mechanotransduction axis promoted extracellular matrix (ECM) metabolism and thus jointly promoted IVD regeneration. After injection into an IDD rat model, the composite hydrogel inhibited the local inflammatory response by inducing macrophage M2 polarization and gradually reducing the ECM degradation. In this study, we propose a fucoidan-DexMA composite hydrogel, which provides an attractive approach for IVD regeneration.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"14 ","pages":"20417314231180050"},"PeriodicalIF":8.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7c/b1/10.1177_20417314231180050.PMC10328174.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10292281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut-on-a-chip for disease models.","authors":"Changxiu Xian,&nbsp;Jiaxin Zhang,&nbsp;Suqing Zhao,&nbsp;Xiang-Guang Li","doi":"10.1177/20417314221149882","DOIUrl":"https://doi.org/10.1177/20417314221149882","url":null,"abstract":"<p><p>The intestinal tract is a vital organ responsible for digestion and absorption in the human body and plays an essential role in pathogen invasion. Compared with other traditional models, gut-on-a-chip has many unique advantages, and thereby, it can be considered as a novel model for studying intestinal functions and diseases. Based on the chip design, we can replicate the in vivo microenvironment of the intestine and study the effects of individual variables on the experiment. In recent years, it has been used to study several diseases. To better mimic the intestinal microenvironment, the structure and function of gut-on-a-chip are constantly optimised and improved. Owing to the complexity of the disease mechanism, gut-on-a-chip can be used in conjunction with other organ chips. In this review, we summarise the human intestinal structure and function as well as the development and improvement of gut-on-a-chip. Finally, we present and discuss gut-on-a-chip applications in inflammatory bowel disease (IBD), viral infections and phenylketonuria. Further improvement of the simulation and high throughput of gut-on-a-chip and realisation of personalised treatments are the problems that should be solved for gut-on-a-chip as a disease model.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"14 ","pages":"20417314221149882"},"PeriodicalIF":8.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/07/3c/10.1177_20417314221149882.PMC9869227.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10677636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}