Modeling of solar UV-induced photodamage on the hair follicles in human skin organoids.

IF 6.7 1区 工程技术 Q1 CELL & TISSUE ENGINEERING
Journal of Tissue Engineering Pub Date : 2024-05-08 eCollection Date: 2024-01-01 DOI:10.1177/20417314241248753
Min-Ji Kim, Hee-Jin Ahn, Dasom Kong, Seunghee Lee, Da-Hyun Kim, Kyung-Sun Kang
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Abstract

Solar ultraviolet (sUV) exposure is known to cause skin damage. However, the pathological mechanisms of sUV on hair follicles have not been extensively explored. Here, we established a model of sUV-exposed skin and its appendages using human induced pluripotent stem cell-derived skin organoids with planar morphology containing hair follicles. Our model closely recapitulated several symptoms of photodamage, including skin barrier disruption, extracellular matrix degradation, and inflammatory response. Specifically, sUV induced structural damage and catagenic transition in hair follicles. As a potential therapeutic agent for hair follicles, we applied exosomes isolated from human umbilical cord blood-derived mesenchymal stem cells to sUV-exposed organoids. As a result, exosomes effectively alleviated inflammatory responses by inhibiting NF-κB activation, thereby suppressing structural damage and promoting hair follicle regeneration. Ultimately, our model provided a valuable platform to mimic skin diseases, particularly those involving hair follicles, and to evaluate the efficacy and underlying mechanisms of potential therapeutics.

模拟太阳紫外线对人体皮肤器官组织中毛囊的光损伤。
众所周知,太阳紫外线(sUV)照射会造成皮肤损伤。然而,人们尚未广泛探讨紫外线对毛囊的病理机制。在这里,我们利用人体诱导多能干细胞衍生的、具有平面形态的、含有毛囊的皮肤器官组织,建立了一个受紫外线照射的皮肤及其附属器官模型。我们的模型密切再现了光损伤的几种症状,包括皮肤屏障破坏、细胞外基质降解和炎症反应。特别是,紫外线会诱导毛囊结构损伤和衰老转变。作为一种潜在的毛囊治疗剂,我们将从人脐带血间充质干细胞中分离出的外泌体应用于紫外线照射下的毛囊组织。结果,外泌体通过抑制NF-κB活化有效缓解了炎症反应,从而抑制了结构损伤并促进了毛囊再生。最终,我们的模型为模拟皮肤病(尤其是涉及毛囊的皮肤病)以及评估潜在疗法的疗效和基本机制提供了一个宝贵的平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Tissue Engineering
Journal of Tissue Engineering Engineering-Biomedical Engineering
CiteScore
11.60
自引率
4.90%
发文量
52
审稿时长
12 weeks
期刊介绍: The Journal of Tissue Engineering (JTE) is a peer-reviewed, open-access journal dedicated to scientific research in the field of tissue engineering and its clinical applications. Our journal encompasses a wide range of interests, from the fundamental aspects of stem cells and progenitor cells, including their expansion to viable numbers, to an in-depth understanding of their differentiation processes. Join us in exploring the latest advancements in tissue engineering and its clinical translation.
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