Journal of Tissue Engineering最新文献_第6页

Revolutionizing digestive system tumor organoids research: Exploring the potential of tumor organoids. 消化系统肿瘤器官组织研究的革命性突破：探索肿瘤器官组织的潜力。

IF 8.2 1区工程技术

Journal of Tissue Engineering Pub Date : 2024-05-27 eCollection Date: 2024-01-01 DOI: 10.1177/20417314241255470

Zhian Xiu, Qian Yang, Fusheng Xie, Feng Han, Weiwei He, Weifang Liao

{"title":"Revolutionizing digestive system tumor organoids research: Exploring the potential of tumor organoids.","authors":"Zhian Xiu, Qian Yang, Fusheng Xie, Feng Han, Weiwei He, Weifang Liao","doi":"10.1177/20417314241255470","DOIUrl":"10.1177/20417314241255470","url":null,"abstract":"Digestive system tumors are the leading cause of cancer-related deaths worldwide. Despite ongoing research, our understanding of their mechanisms and treatment remain inadequate. One promising tool for clinical applications is the use of gastrointestinal tract tumor organoids, which serve as an important in vitro model. Tumor organoids exhibit a genotype similar to the patient's tumor and effectively mimic various biological processes, including tissue renewal, stem cell, and ecological niche functions, and tissue response to drugs, mutations, or injury. As such, they are valuable for drug screening, developing novel drugs, assessing patient outcomes, and supporting immunotherapy. In addition, innovative materials and techniques can be used to optimize tumor organoid culture systems. Several applications of digestive system tumor organoids have been described and have shown promising results in related aspects. In this review, we discuss the current progress, limitations, and prospects of this model for digestive system tumors.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"15 ","pages":"20417314241255470"},"PeriodicalIF":8.2,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11131411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Timely delivery of bone marrow mesenchymal stem cells based on the inflammatory pattern of bone injury environment to promote the repair of calvarial bone defects in rats: An optimized strategy for bone tissue engineering 根据骨损伤环境的炎症模式及时输送骨髓间充质干细胞，促进大鼠腓骨缺损的修复：骨组织工程的优化策略

IF 8.2 1区工程技术

Journal of Tissue Engineering Pub Date : 2024-05-17 DOI: 10.1177/20417314241252960

Yuwei Zeng, Aiju Lou, Zhenmin Zhong, Yu Cai, Yixi Yang, Haifeng Liang, Yucong Lin, Zhuoxuan He, Lei Zhou, Zhi-Yong Zhang, Le Wang

{"title":"Timely delivery of bone marrow mesenchymal stem cells based on the inflammatory pattern of bone injury environment to promote the repair of calvarial bone defects in rats: An optimized strategy for bone tissue engineering","authors":"Yuwei Zeng, Aiju Lou, Zhenmin Zhong, Yu Cai, Yixi Yang, Haifeng Liang, Yucong Lin, Zhuoxuan He, Lei Zhou, Zhi-Yong Zhang, Le Wang","doi":"10.1177/20417314241252960","DOIUrl":"https://doi.org/10.1177/20417314241252960","url":null,"abstract":"Stem cell-based therapy plays a significant role in the repair of bone defects. However, traditional stem cell transplantation strategies in bone tissue engineering are characterized by low survival rates and unstable treatment outcomes. In this study, we propose a timely delivery strategy for inflammatory changes in the setting of bone injury to improve the survival rate of transplanted cells and bone repair. The results of cell tracing in vivo showed that this strategy could effectively improve the survival rate of low-dose exogenous transplanted cells in bone defect areas, and CD31 immunofluorescence and histological sections suggested that this strategy effectively promoted vascularization and new bone formation in the calvarial defect area. Subsequently, we analyzed the mechanism of action of the “Two-step” strategy from the perspective of inflammatory microenvironment regulation, and the results suggested that the first batch transplanted stem cells caused localized and transient increases in tissue apoptosis levels and inflammatory factors, and recruited macrophage chemotaxis, and the second batch of cells may promote pro-inflammatory - anti-inflammatory transformation of the tissue. Finally, mRNA sequencing results suggest that the first batch cells in the “Two-step” strategy are important initiators in bone repair, which not only actively regulate the immune microenvironment at the bone defect, but also guide richer cellular activity and more positive biochemical responses. Therefore, the “Two-step” strategy leads to efficient inflammatory environment regulation and superior bone repair effects, which may provide an alternative option for the treatment of bone defects in the future.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"139 1","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141061314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling of solar UV-induced photodamage on the hair follicles in human skin organoids. 模拟太阳紫外线对人体皮肤器官组织中毛囊的光损伤。

IF 8.2 1区工程技术

Journal of Tissue Engineering Pub Date : 2024-05-08 eCollection Date: 2024-01-01 DOI: 10.1177/20417314241248753

Min-Ji Kim, Hee-Jin Ahn, Dasom Kong, Seunghee Lee, Da-Hyun Kim, Kyung-Sun Kang

{"title":"Modeling of solar UV-induced photodamage on the hair follicles in human skin organoids.","authors":"Min-Ji Kim, Hee-Jin Ahn, Dasom Kong, Seunghee Lee, Da-Hyun Kim, Kyung-Sun Kang","doi":"10.1177/20417314241248753","DOIUrl":"10.1177/20417314241248753","url":null,"abstract":"Solar ultraviolet (sUV) exposure is known to cause skin damage. However, the pathological mechanisms of sUV on hair follicles have not been extensively explored. Here, we established a model of sUV-exposed skin and its appendages using human induced pluripotent stem cell-derived skin organoids with planar morphology containing hair follicles. Our model closely recapitulated several symptoms of photodamage, including skin barrier disruption, extracellular matrix degradation, and inflammatory response. Specifically, sUV induced structural damage and catagenic transition in hair follicles. As a potential therapeutic agent for hair follicles, we applied exosomes isolated from human umbilical cord blood-derived mesenchymal stem cells to sUV-exposed organoids. As a result, exosomes effectively alleviated inflammatory responses by inhibiting NF-κB activation, thereby suppressing structural damage and promoting hair follicle regeneration. Ultimately, our model provided a valuable platform to mimic skin diseases, particularly those involving hair follicles, and to evaluate the efficacy and underlying mechanisms of potential therapeutics.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"15 ","pages":"20417314241248753"},"PeriodicalIF":8.2,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11080775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Additively manufactured bioceramic scaffolds based on triply periodic minimal surfaces for bone regeneration 基于三周期性最小表面的添加式制造生物陶瓷支架用于骨再生

IF 8.2 1区工程技术

Journal of Tissue Engineering Pub Date : 2024-04-12 DOI: 10.1177/20417314241244997

Hong Zhu, Jinsi Wang, Shengfa Wang, Yue Yang, Meiyi Chen, Qifei Luan, Xiaochuan Liu, Ziheng Lin, Jiaqi Hu, Kenny Man, Jingying Zhang

{"title":"Additively manufactured bioceramic scaffolds based on triply periodic minimal surfaces for bone regeneration","authors":"Hong Zhu, Jinsi Wang, Shengfa Wang, Yue Yang, Meiyi Chen, Qifei Luan, Xiaochuan Liu, Ziheng Lin, Jiaqi Hu, Kenny Man, Jingying Zhang","doi":"10.1177/20417314241244997","DOIUrl":"https://doi.org/10.1177/20417314241244997","url":null,"abstract":"The study focused on the effects of a triply periodic minimal surface (TPMS) scaffolds, varying in porosity, on the repair of mandibular defects in New Zealand white rabbits. Four TPMS configurations (40%, 50%, 60%, and 70% porosity) were fabricated with β-tricalcium phosphate bioceramic via additive manufacturing. Scaffold properties were assessed through scanning electron microscopy and mechanical testing. For proliferation and adhesion assays, mouse bone marrow stem cells (BMSCs) were cultured on these scaffolds. In vivo, the scaffolds were implanted into rabbit mandibular defects for 2 months. Histological staining evaluated osteogenic potential. Moreover, RNA-sequencing analysis and RT-qPCR revealed the significant involvement of angiogenesis-related factors and Hippo signaling pathway in influencing BMSCs behavior. Notably, the 70% porosity TPMS scaffold exhibited optimal compressive strength, superior cell proliferation, adhesion, and significantly enhanced osteogenesis and angiogenesis. These findings underscore the substantial potential of 70% porosity TPMS scaffolds in effectively promoting bone regeneration within mandibular defects.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"5 1","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140575709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro? 脑组织模型开发的当前进展与挑战：如何在体外培养可变化的大脑？

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2024-03-20 eCollection Date: 2024-01-01 DOI: 10.1177/20417314241235527

Alla B Salmina, Olga P Alexandrova, Anton S Averchuk, Sofia A Korsakova, Mikis R Saridis, Sergey N Illarioshkin, Stanislav O Yurchenko

{"title":"Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro?","authors":"Alla B Salmina, Olga P Alexandrova, Anton S Averchuk, Sofia A Korsakova, Mikis R Saridis, Sergey N Illarioshkin, Stanislav O Yurchenko","doi":"10.1177/20417314241235527","DOIUrl":"10.1177/20417314241235527","url":null,"abstract":"In vitro modeling of brain tissue is a promising but not yet resolved problem in modern neurobiology and neuropharmacology. Complexity of the brain structure and diversity of cell-to-cell communication in (patho)physiological conditions make this task almost unachievable. However, establishment of novel in vitro brain models would ultimately lead to better understanding of development-associated or experience-driven brain plasticity, designing efficient approaches to restore aberrant brain functioning. The main goal of this review is to summarize the available data on methodological approaches that are currently in use, and to identify the most prospective trends in development of neurovascular unit, blood-brain barrier, blood-cerebrospinal fluid barrier, and neurogenic niche in vitro models. The manuscript focuses on the regulation of adult neurogenesis, cerebral microcirculation and fluids dynamics that should be reproduced in the in vitro 4D models to mimic brain development and its alterations in brain pathology. We discuss approaches that are critical for studying brain plasticity, deciphering the individual person-specific trajectory of brain development and aging, and testing new drug candidates in the in vitro models.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"15 ","pages":"20417314241235527"},"PeriodicalIF":6.7,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10956167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140186833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanodrug delivery systems for regulating microglial polarization in ischemic stroke treatment: A review. 在缺血性中风治疗中调节小胶质细胞极化的纳米药物输送系统：综述。

IF 8.2 1区工程技术

Journal of Tissue Engineering Pub Date : 2024-03-13 eCollection Date: 2024-01-01 DOI: 10.1177/20417314241237052

Shuang-Yin Lei, Yu-Qian Yang, Jia-Cheng Liu, Dian-Hui Zhang, Yang Qu, Ying-Ying Sun, Hong-Jing Zhu, Sheng-Yu Zhou, Yi Yang, Zhen-Ni Guo

{"title":"Nanodrug delivery systems for regulating microglial polarization in ischemic stroke treatment: A review.","authors":"Shuang-Yin Lei, Yu-Qian Yang, Jia-Cheng Liu, Dian-Hui Zhang, Yang Qu, Ying-Ying Sun, Hong-Jing Zhu, Sheng-Yu Zhou, Yi Yang, Zhen-Ni Guo","doi":"10.1177/20417314241237052","DOIUrl":"10.1177/20417314241237052","url":null,"abstract":"The incidence of ischemic stroke (IS) is rising in tandem with the global aging population. There is an urgent need to delve deeper into the pathological mechanisms and develop new neuroprotective strategies. In the present review, we discuss the latest advancements and research on various nanodrug delivery systems (NDDSs) for targeting microglial polarization in IS treatment. Furthermore, we critically discuss the different strategies. NDDSs have demonstrated exceptional qualities to effectively permeate the blood-brain barrier, aggregate at the site of ischemic injury, and target specific cell types within the brain when appropriately modified. Consequently, NDDSs have considerable potential for reshaping the polarization phenotype of microglia and could be a prospective therapeutic strategy for IS. The treatment of IS remains a challenge. However, this review provides a new perspective on neuro-nanomedicine for IS therapies centered on microglial polarization, thereby inspiring new research ideas and directions.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"15 ","pages":"20417314241237052"},"PeriodicalIF":8.2,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10935760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing pre-clinical research with simplified intestinal cell line models 利用简化的肠细胞系模型加强临床前研究

IF 8.2 1区工程技术

Journal of Tissue Engineering Pub Date : 2024-03-05 DOI: 10.1177/20417314241228949

Christina Fey, Theresa Truschel, Kristina Nehlsen, Spyridon Damigos, Julia Horstmann, Theresia Stradal, Tobias May, Marco Metzger, Daniela Zdzieblo

{"title":"Enhancing pre-clinical research with simplified intestinal cell line models","authors":"Christina Fey, Theresa Truschel, Kristina Nehlsen, Spyridon Damigos, Julia Horstmann, Theresia Stradal, Tobias May, Marco Metzger, Daniela Zdzieblo","doi":"10.1177/20417314241228949","DOIUrl":"https://doi.org/10.1177/20417314241228949","url":null,"abstract":"Two-dimensional culture remains widely employed to determine the bioavailability of orally delivered drugs. To gain more knowledge about drug uptake mechanisms and risk assessment for the patient after oral drug admission, intestinal in vitro models demonstrating a closer similarity to the in vivo situation are needed. In particular, Caco-2 cell-based Transwell® models show advantages as they are reproducible, cost-efficient, and standardized. However, cellular complexity is impaired and cell function is strongly modified as important transporters in the apical membrane are missing. To overcome these limitations, primary organoid-based human small intestinal tissue models were developed recently but the application of these cultures in pre-clinical research still represents an enormous challenge, as culture setup is complex as well as time- and cost-intensive. To overcome these hurdles, we demonstrate the establishment of primary organoid-derived intestinal cell lines by immortalization. Besides exhibiting cellular diversity of the organoid, these immortalized cell lines enable a standardized and more cost-efficient culture. Further, our cell line-based Transwell®-like models display an organ-specific epithelial barrier integrity, ultrastructural features and representative transport functions. Altogether, our novel model systems are cost-efficient with close similarity to the in vivo situation, therefore favoring their use in bioavailability studies in the context of pre-clinical screenings.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"32 1","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140044537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced lung organoids for respiratory system and pulmonary disease modeling 用于呼吸系统和肺部疾病建模的先进肺器官模型

IF 8.2 1区工程技术

Journal of Tissue Engineering Pub Date : 2024-02-23 DOI: 10.1177/20417314241232502

Hyebin Joo, Sungjin Min, Seung-Woo Cho

{"title":"Advanced lung organoids for respiratory system and pulmonary disease modeling","authors":"Hyebin Joo, Sungjin Min, Seung-Woo Cho","doi":"10.1177/20417314241232502","DOIUrl":"https://doi.org/10.1177/20417314241232502","url":null,"abstract":"Amidst the recent coronavirus disease 2019 (COVID-19) pandemic, respiratory system research has made remarkable progress, particularly focusing on infectious diseases. Lung organoid, a miniaturized structure recapitulating lung tissue, has gained global attention because of its advantages over other conventional models such as two-dimensional (2D) cell models and animal models. Nevertheless, lung organoids still face limitations concerning heterogeneity, complexity, and maturity compared to the native lung tissue. To address these limitations, researchers have employed co-culture methods with various cell types including endothelial cells, mesenchymal cells, and immune cells, and incorporated bioengineering platforms such as air-liquid interfaces, microfluidic chips, and functional hydrogels. These advancements have facilitated applications of lung organoids to studies of pulmonary diseases, providing insights into disease mechanisms and potential treatments. This review introduces recent progress in the production methods of lung organoids, strategies for improving maturity, functionality, and complexity of organoids, and their application in disease modeling, including respiratory infection and pulmonary fibrosis.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"30 1","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139948489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of the immune microenvironment by pioglitazone-loaded polylactic glycolic acid nanosphere composite scaffolds to promote vascularization and bone regeneration. 利用吡格列酮负载的聚乳酸乙醇酸纳米复合支架调节免疫微环境，促进血管生成和骨再生。

IF 8.2 1区工程技术

Journal of Tissue Engineering Pub Date : 2024-02-14 eCollection Date: 2024-01-01 DOI: 10.1177/20417314241231452

Shijie Fan, Yadong Tan, Xiuchen Yuan, Chun Liu, Xiaoyu Wu, Ting Dai, Su Ni, Jiafeng Wang, Yiping Weng, Hongbin Zhao

{"title":"Regulation of the immune microenvironment by pioglitazone-loaded polylactic glycolic acid nanosphere composite scaffolds to promote vascularization and bone regeneration.","authors":"Shijie Fan, Yadong Tan, Xiuchen Yuan, Chun Liu, Xiaoyu Wu, Ting Dai, Su Ni, Jiafeng Wang, Yiping Weng, Hongbin Zhao","doi":"10.1177/20417314241231452","DOIUrl":"10.1177/20417314241231452","url":null,"abstract":"Osteogenesis is caused by multiple factors, and the inflammatory response, osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), regeneration of blood vessels, and other factors must be considered in bone tissue engineering. To effectively repair bone defect, it is important to decrease excessive inflammation, enhance the differentiation of mesenchymal stem cells into osteoblasts, and stimulate angiogenesis. Herein, nano-attapulgite (ATP), polyvinyl alcohol (PVA), and gelatin (GEL) scaffolds were produced using 3D printing technology and pioglitazone (PIO)-containing polylactic acid-glycolic acid (PLGA) nanospheres were added. In both in vitro and in vivo studies, material scaffolds with PIO-loaded polylactic acid-glycolic acid nanospheres could reduce the inflammatory response by encouraging macrophage polarization from M1 to M2 and promoting the osteogenic differentiation of BMSCs by activating the BMP2/Smad/RUNX2 signal pathway to repair bone defects. The vascularization of human umbilical vein endothelial cells (HUVECs) through the PI3K/AKT/HIF1-/VEGF pathway was also encouraged. In vivo research using PIO-containing PLGA nanospheres revealed massive collagen deposition in skin models. These findings indicate a potentially effective scaffold for bone healing, when PLGA nanospheres-which contain the drug PIO-are combined with ATP/PVA/GEL scaffolds.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"15 ","pages":"20417314241231452"},"PeriodicalIF":8.2,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10868507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139741365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human induced pluripotent stem cell-derived planar neural organoids assembled on synthetic hydrogels. 组装在合成水凝胶上的人诱导多能干细胞衍生的平面神经器官组织。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2024-02-14 eCollection Date: 2024-01-01 DOI: 10.1177/20417314241230633

Joydeb Majumder, Elizabeth E Torr, Elizabeth A Aisenbrey, Connie S Lebakken, Peter F Favreau, William D Richards, Yanhong Yin, Qiang Chang, William L Murphy

{"title":"Human induced pluripotent stem cell-derived planar neural organoids assembled on synthetic hydrogels.","authors":"Joydeb Majumder, Elizabeth E Torr, Elizabeth A Aisenbrey, Connie S Lebakken, Peter F Favreau, William D Richards, Yanhong Yin, Qiang Chang, William L Murphy","doi":"10.1177/20417314241230633","DOIUrl":"10.1177/20417314241230633","url":null,"abstract":"The tailorable properties of synthetic polyethylene glycol (PEG) hydrogels make them an attractive substrate for human organoid assembly. Here, we formed human neural organoids from iPSC-derived progenitor cells in two distinct formats: (i) cells seeded on a Matrigel surface; and (ii) cells seeded on a synthetic PEG hydrogel surface. Tissue assembly on synthetic PEG hydrogels resulted in three dimensional (3D) planar neural organoids with greater neuronal diversity, greater expression of neurovascular and neuroinflammatory genes, and reduced variability when compared with tissues assembled upon Matrigel. Further, our 3D human tissue assembly approach occurred in an open cell culture format and created a tissue that was sufficiently translucent to allow for continuous imaging. Planar neural organoids formed on PEG hydrogels also showed higher expression of neural, vascular, and neuroinflammatory genes when compared to traditional brain organoids grown in Matrigel suspensions. Further, planar neural organoids contained functional microglia that responded to pro-inflammatory stimuli, and were responsive to anti-inflammatory drugs. These results demonstrate that the PEG hydrogel neural organoids can be used as a physiologically relevant in vitro model of neuro-inflammation.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"15 ","pages":"20417314241230633"},"PeriodicalIF":6.7,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10868488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139741364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0