Journal of Research in Pharmacy最新文献_第5页

Anti-inflammatory effects of velvet bean (Mucuna pruriens L. (DC.), Fabaceae) leaf ethanolic extract against carrageenan in male mice 蚕豆(Mucuna pruriens L. (DC.)，豆科)叶乙醇提取物对雄性小鼠卡拉胶的抗炎作用

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Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.438

Fadilaturahmah Fadilaturahmah, Resti Rahayu, P. Santoso

引用次数: 0

Antibacterial Properties of Carvacrol against Antibiotic- Resistant Bacteria, Enteric Bacteria, and Oral Pathogens 香芹酚对耐药细菌、肠道细菌和口腔病原体的抗菌性能

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Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.425

A. Hoş, P. Özgen, A. Inci, Buse Avci, Ceyda Ceylan

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Burn assessment: A critical review on care, advances in burn healing and pre-clinical animal studies 烧伤评估:对护理，烧伤愈合和临床前动物研究进展的重要回顾

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Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.443

Ravish J. Patel, Rashesh Desai, Amit V. Patel, Shailvi Shah, B. Prajapati, Viral A. Patel, A. Alexander

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Prospects for repurposing FDA-approved medications as Omicron spike/ACE-2 protein complex disruptors fda批准的药物作为欧米克隆刺突/ACE-2蛋白复合物干扰物的前景

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Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.445

Jaikanth Chandrasekaran, P. Parasuraman, Panneerselvam Theivendren, K. Sundar, Damodar Nayak Ammunje, Rex Devasahayam Arokia Balaya, Selvaraj Kunjiappan

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Method Development and Validation for Tenofovir an Antiretroviral Drug in Plasma by LC-MS/MS Technique 方法采用LC-MS/MS技术建立抗逆转录病毒药物替诺福韦的血浆检测方法并进行验证

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Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.463

Sarang Salunke, Sagar Wankhede, S. Medhe, H. Nimje, Samir Ranjan, Vikas Kendre, Payal Bhaskar

{"title":"Method Development and Validation for Tenofovir an Antiretroviral Drug in Plasma by LC-MS/MS Technique","authors":"Sarang Salunke, Sagar Wankhede, S. Medhe, H. Nimje, Samir Ranjan, Vikas Kendre, Payal Bhaskar","doi":"10.29228/jrp.463","DOIUrl":"https://doi.org/10.29228/jrp.463","url":null,"abstract":": Bioanalytical method development for Tenofovir (TFR) as an antiretroviral drug by LCMS Technique. A developed Bioanalytical analysis method for TFR can be used routinely in a commercial laboratory. All the solvents used were of HPLC grade. 4000 QTrap along with the Shimadzu LC 20AD LC System used to develop and validate the method. The LLOQ and LOQ for Tenofovir was found were 5ng/mL and 15ng/mL. The method was accurate (within ±15% of control) and precise (coefficient of variation ≤ 15%). Analytes were stable for five freeze/thaw cycles and up to 6 days at room temperature, whereas long-term at − 20°C or at − 80°C. For Precision study using QCs of the drug-85%, 100% and 115% concentration of drug chosen and the levels M1QC (75ng/mL), MQC (300ng/mL) and HQC (600ng/mL) where the % CV were observed of ≤ 15%. In a Precision and Accuracy study (inter day and intraday), the % CV obtained for Tenofovir was observed ≤ 15%. Recovery studies for extracted samples with LQC (15ng/mL), MQC (300ng/mL) and HQC (600ng/mL) were 94.51%, 91.83% and 90.91% respectively. Stability was within 15% deviation. The results of System Suitability Test for TFR and Acyclovir (ACR) are an internal standard with observed %CV ≤ 2.0%. The aim of the study was to develop a method that could be used as an alternative to the existing Tenofovir indirect method. The existing method observes separating the parent drug from the metabolite in LCMS/MS. This method is a good alternative to the indirect methods currently in use.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"26 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts. 蛇麻提取物体外细胞毒性评价及植物化学分析。

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.490

Aysegul CASKURLU, Sule Nur KARAVUS, Sevde Nur BİLTEKİN, Esra Zeynep HELVACI, Ayse Esra KARADAG

{"title":"In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts.","authors":"Aysegul CASKURLU, Sule Nur KARAVUS, Sevde Nur BİLTEKİN, Esra Zeynep HELVACI, Ayse Esra KARADAG","doi":"10.29228/jrp.490","DOIUrl":"https://doi.org/10.29228/jrp.490","url":null,"abstract":": The aim of this study was to evaluate phytochemical composition of Ajuga reptans L. (Lamiaceae) aerial parts (separately flower and leaf) methanol, aqueous-methanolic extracts, and their cytotoxic activities. The phytochemical analysis was performed high-performance liquid chromatography (HPLC). Caffeic acid, p -coumaric, gallic, chlorogenic, ferulic acids, kaempferol, rutin, quercetin, quercetin-3-O-galactoside, and quercitrin were used as reference substances by HPLC in all samples. The major compounds in the extract were found as ferulic acid, caffeic acid, rutin, and quercetin-3-O-galactoside. Cytotoxicity was investigated using methyl thiazole tetrazolium (MTT) assay. Cytotoxic evaluation of the extracts against cancer (MCF7, PC3, and A549) and healthy human embryonic kidney cell line (HEK293) cell lines by MTT. Compared to other cells, the methanol extract of A. reptans demonstrated high selectivity against PC3 cells (IC 50 : 95 ± 0.99 µg/mL) and selectivity index was four times higher than reference drug colchicine. (IC 50 : 95 ± 0.99 µg/mL, SI: 6.10). A. reptans demonstrated antiproliferative potential against prostate and lung cancer cells. Therefore, additional investigations are needed to study the mechanism of the cytotoxicity for A. reptans .","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"242 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135440463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Steam bath, vibration, and thermal ablation administrations augment the release of tramadol HCl from transdermal patch and enhance the plasma concentration in rats. 蒸汽浴、振动和热消融处理增加了曲马多盐酸从透皮贴片的释放，并提高了大鼠血浆浓度。

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.496

Caglar MACIT, Gulengul DUMAN, Meltem MACIT

引用次数: 0

Production and characterization of newly developed alcohol-free topical liposome-gel transdermal drug delivery systems containing estradiol (E2)/ estriol (E3) for post-menopausal women 新开发的含雌二醇(E2)/雌三醇(E3)的无醇外用脂质体凝胶经皮给药系统的生产和表征

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.499

İsmail ASLAN, Ali Fuat AYTEKİN

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GROUNDBREAKING DELIVERY SYSTEMS: LIPOSOME -MICROBUBBLES COMPLEXES 突破性的输送系统:脂质体-微泡复合物

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.508

Pankaj DWIVEDI

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In silico evaluation of potential murine M49 DNA aptamer on ORF7a of SARS-COV-2: A similar target 在SARS-COV-2的ORF7a上潜在的小鼠M49 DNA适体的计算机评价:一个类似的靶标

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Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.306

Nor Azlina AHMAD, R. M. Zulkifli, H. Hussin, S. Amran, M. Nadri, Saiful Izwan Abd Razak, Sabrina Adam, F. M. Yusof

{"title":"In silico evaluation of potential murine M49 DNA aptamer on ORF7a of SARS-COV-2: A similar target","authors":"Nor Azlina AHMAD, R. M. Zulkifli, H. Hussin, S. Amran, M. Nadri, Saiful Izwan Abd Razak, Sabrina Adam, F. M. Yusof","doi":"10.29228/jrp.306","DOIUrl":"https://doi.org/10.29228/jrp.306","url":null,"abstract":"DNA aptamers are short nucleotides with a high affinity for their target. However, the process of isolating aptamers via the systematic evolution of ligands by exponential enrichment (SELEX) procedure is laborious. Therefore, an in silico approach is used to screen potential DNA aptamer candidates as a kickstart specifically for ORF7a of SARSCOV-2. By applying the TM-align program, the murine receptor (CD200R) protein was found to have structural similarities with ORF7a. Based on the literature, this CD200R protein is successfully bound by M49 DNA aptamers experimentally. Herein, the 3D structure of the M49 DNA aptamer was generated using Mfold, RNA Composer webserver, Discovery Studio Visualizer, and UCSF Chimera software, and the docking simulation was predicted using the HDOCK webserver. The binding energy scores for the M49-CD200R complex were slightly higher than those for the M49-ORF7a complex with-233.78 and-220.11, respectively. The molecular interaction in the complexes was contributed by the hydrogen bond. In conclusion, the M49 aptamer of CD200R protein can bind to the other similar target, the ORF7a protein of SARS-COV-2. Even though CD200R and ORF7a proteins share structural similarities, the binding sites of the individual complex are distinct. The current study shows that two different proteins with structural similarities may have a possibility to share the same DNA aptamer. This strategy may result in efficient aptamer discovery using an in silico method as a first step. © 2022 Marmara University Press.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69836565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0