Journal of Research in Pharmacy最新文献_第6页

Steam bath, vibration, and thermal ablation administrations augment the release of tramadol HCl from transdermal patch and enhance the plasma concentration in rats. 蒸汽浴、振动和热消融处理增加了曲马多盐酸从透皮贴片的释放，并提高了大鼠血浆浓度。

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.496

Caglar MACIT, Gulengul DUMAN, Meltem MACIT

引用次数: 0

Production and characterization of newly developed alcohol-free topical liposome-gel transdermal drug delivery systems containing estradiol (E2)/ estriol (E3) for post-menopausal women 新开发的含雌二醇(E2)/雌三醇(E3)的无醇外用脂质体凝胶经皮给药系统的生产和表征

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.499

İsmail ASLAN, Ali Fuat AYTEKİN

引用次数: 0

GROUNDBREAKING DELIVERY SYSTEMS: LIPOSOME -MICROBUBBLES COMPLEXES 突破性的输送系统:脂质体-微泡复合物

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.508

Pankaj DWIVEDI

引用次数: 0

Goji berry fruit extracts induce cytotoxicity and apoptotic cell death in breast cancer cells 枸杞提取物诱导乳腺癌细胞毒性和凋亡细胞死亡

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.459

S. Gezici

{"title":"Goji berry fruit extracts induce cytotoxicity and apoptotic cell death in breast cancer cells","authors":"S. Gezici","doi":"10.29228/jrp.459","DOIUrl":"https://doi.org/10.29228/jrp.459","url":null,"abstract":": Anti-cancer agents derived from dietary medicinal plants are of great interest among people. The fruits of the Goji berry ( Lycium barbarum L., Fam. Solanaceae), also known as the ‘King of the Berries’, have valuable health benefits and pharmacological properties thanks to their rich content of phytochemicals. Considering the wide range of biological and pharmacological properties of goji berries, it was aimed to investigate the anticancer, antiproliferative and apoptotic cell death activities against human breast adenocarcinoma and carcinoma cells, MCF-7 and T47D, respectively. In the present study, the anticancer activities of the extracts were evaluated using MTT assay, the antiproliferative effects were investigated using bromodeoxyuridine (BrdU) cell proliferation assay, and the apoptotic cell death activities of the extracts were analyzed by immunological-based ELISA. As the main result of this research, it was found that extracts of goji berries significantly decreased the cell viability of breast cancer cells and caused these cells to undergo apoptosis-mediated cell death in a dose-and time-dependent manner. Among the tested extracts, methanol extracts showed the highest anticancer activity against MCF-7 and T47D cells (IC 50 =54.06±0.05 µ g/mL and 76.14±0.38 µ g/mL, p<0.01, respectively), while the lowest activity was observed in goji berry extracts prepared with dichloromethane (IC 50 =101.05±0.14 µ g/mL, p<0.05 and 124.10±0.86 µ g/mL, p<0.01, respectively). Moreover, the methanol extracts caused the strongest antiproliferative activity in MCF-7 cells (p<0.05). Regarding the apoptotic cell death potential of the extracts, increased apoptotic cell death was observed in both breast cancer cells, however, apoptotic cell death occurred more strongly in MCF-7 cells than in T47D cells. Consequently, this study suggests that goji berries could be valuable natural sources for the development of herbal formulations against breast cancer. Accordingly, further detailed studies should be conducted to elucidate the molecular signaling pathways and mechanisms of action.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Cornus mas L. on lipid peroxidation and anti-oxidative enzyme activity in high fat diet fed rats 山茱萸对高脂日粮大鼠脂质过氧化和抗氧化酶活性的影响

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.325

D. Kargın, Şule Aktaç, A. Yavuz, M. Cam

引用次数: 0

Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats 芦荟主要酚类化合物的鉴定及其对糖尿病大鼠卵巢氧化应激的保护作用

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.348

S. Kurt, U. Şeker, M. Yazlık, Engin Er, M. Hürkul, S. Soker

引用次数: 1

Investigation of the anticancer effects of some plant seed oils with medicinal uses 几种药用植物籽油抗癌作用的研究

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.444

Yener Kurman, Görkem Dülger, Ö. Pasin

引用次数: 0

QbD based approach to RP-HPLC method development and validation of Bupivacaine hydrochloride in bulk and in-house developed nanostructured lipid carriers 基于QbD的反相高效液相色谱法开发和验证盐酸布比卡因散装和自主开发的纳米结构脂质载体

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.475

Sarfaraz Khan, F. Khan

{"title":"QbD based approach to RP-HPLC method development and validation of Bupivacaine hydrochloride in bulk and in-house developed nanostructured lipid carriers","authors":"Sarfaraz Khan, F. Khan","doi":"10.29228/jrp.475","DOIUrl":"https://doi.org/10.29228/jrp.475","url":null,"abstract":": The present study describes the principles of systematic Quality by design (QbD) approach for the development of RP-HPLC method for the quantification of Bupivacaine hydrochloride (BUP) in bulk and in-house developed nanostructured lipid carriers (NLCs). Initially analytical target profile (ATP), and critical analytical attributes (CAAs) were identified. Primary assessment studies were checked using Plackett-Burman Design. Further optimization studies were performed by applying Box-Behnken design. The Shimadzu C-18 column (250mm x 4.6mm i.d., 5µm particle size) was utilized for reversed-phase chromatographic separation with a mobile phase comprising a mixture of acetonitrile (ACN) and 0.1% ortho phosphoric acid (OPA) (pH 2.04) in 69.45:30.55 (% v/v). The flow rate was 0.805mL/min at a λ max of 214 nm and an injection volume of 12µL. The new developed method was validated according to the guidelines given by International Conference on Harmonization which revealed linearity between 25 to 80µg/ml and r 2 = 0.999. The result of % RSD was 0.38 and 0.44 respectively for high degree of intraday and interday precision. As per the new method the LOD and LOQ is 0.900µg/ml and 2.72µg/ml, respectively. Further the validated method was also applied for the estimation of BUP in NLCs formulation, which showed no interference of any formulation excipients. The studies demonstrated that the new method is rapid, simple, selective, and reproducible for the estimation of pure drug and in-house developed NLCs.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69840002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico evaluation of potential murine M49 DNA aptamer on ORF7a of SARS-COV-2: A similar target 在SARS-COV-2的ORF7a上潜在的小鼠M49 DNA适体的计算机评价:一个类似的靶标

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.306

Nor Azlina AHMAD, R. M. Zulkifli, H. Hussin, S. Amran, M. Nadri, Saiful Izwan Abd Razak, Sabrina Adam, F. M. Yusof

{"title":"In silico evaluation of potential murine M49 DNA aptamer on ORF7a of SARS-COV-2: A similar target","authors":"Nor Azlina AHMAD, R. M. Zulkifli, H. Hussin, S. Amran, M. Nadri, Saiful Izwan Abd Razak, Sabrina Adam, F. M. Yusof","doi":"10.29228/jrp.306","DOIUrl":"https://doi.org/10.29228/jrp.306","url":null,"abstract":"DNA aptamers are short nucleotides with a high affinity for their target. However, the process of isolating aptamers via the systematic evolution of ligands by exponential enrichment (SELEX) procedure is laborious. Therefore, an in silico approach is used to screen potential DNA aptamer candidates as a kickstart specifically for ORF7a of SARSCOV-2. By applying the TM-align program, the murine receptor (CD200R) protein was found to have structural similarities with ORF7a. Based on the literature, this CD200R protein is successfully bound by M49 DNA aptamers experimentally. Herein, the 3D structure of the M49 DNA aptamer was generated using Mfold, RNA Composer webserver, Discovery Studio Visualizer, and UCSF Chimera software, and the docking simulation was predicted using the HDOCK webserver. The binding energy scores for the M49-CD200R complex were slightly higher than those for the M49-ORF7a complex with-233.78 and-220.11, respectively. The molecular interaction in the complexes was contributed by the hydrogen bond. In conclusion, the M49 aptamer of CD200R protein can bind to the other similar target, the ORF7a protein of SARS-COV-2. Even though CD200R and ORF7a proteins share structural similarities, the binding sites of the individual complex are distinct. The current study shows that two different proteins with structural similarities may have a possibility to share the same DNA aptamer. This strategy may result in efficient aptamer discovery using an in silico method as a first step. © 2022 Marmara University Press.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69836565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intranasal Oleic acid-based nanoemulsion of Diazepam: design, formulation and in-vitro evaluation 地西泮鼻内油酸纳米乳:设计、配方及体外评价

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.335

Yousif K. YOUNIS, S. N. A. Alhammid

引用次数: 0