Journal of Research in Pharmacy最新文献_第6页

Intranasal Oleic acid-based nanoemulsion of Diazepam: design, formulation and in-vitro evaluation 地西泮鼻内油酸纳米乳:设计、配方及体外评价

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.335

Yousif K. YOUNIS, S. N. A. Alhammid

引用次数: 0

Effects of Cornus mas L. on lipid peroxidation and anti-oxidative enzyme activity in high fat diet fed rats 山茱萸对高脂日粮大鼠脂质过氧化和抗氧化酶活性的影响

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.325

D. Kargın, Şule Aktaç, A. Yavuz, M. Cam

引用次数: 0

Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats 芦荟主要酚类化合物的鉴定及其对糖尿病大鼠卵巢氧化应激的保护作用

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.348

S. Kurt, U. Şeker, M. Yazlık, Engin Er, M. Hürkul, S. Soker

引用次数: 1

Formulation of an anti-aging cream containing Alteromonas ferment extract and evaluation of the effect on collagen concentration 含异单胞菌发酵提取物的抗衰老乳霜的配方及对胶原蛋白浓度的影响

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.370

G. Gedik

引用次数: 0

Investigation of the anticancer effects of some plant seed oils with medicinal uses 几种药用植物籽油抗癌作用的研究

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.444

Yener Kurman, Görkem Dülger, Ö. Pasin

引用次数: 0

Goji berry fruit extracts induce cytotoxicity and apoptotic cell death in breast cancer cells 枸杞提取物诱导乳腺癌细胞毒性和凋亡细胞死亡

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.459

S. Gezici

{"title":"Goji berry fruit extracts induce cytotoxicity and apoptotic cell death in breast cancer cells","authors":"S. Gezici","doi":"10.29228/jrp.459","DOIUrl":"https://doi.org/10.29228/jrp.459","url":null,"abstract":": Anti-cancer agents derived from dietary medicinal plants are of great interest among people. The fruits of the Goji berry ( Lycium barbarum L., Fam. Solanaceae), also known as the ‘King of the Berries’, have valuable health benefits and pharmacological properties thanks to their rich content of phytochemicals. Considering the wide range of biological and pharmacological properties of goji berries, it was aimed to investigate the anticancer, antiproliferative and apoptotic cell death activities against human breast adenocarcinoma and carcinoma cells, MCF-7 and T47D, respectively. In the present study, the anticancer activities of the extracts were evaluated using MTT assay, the antiproliferative effects were investigated using bromodeoxyuridine (BrdU) cell proliferation assay, and the apoptotic cell death activities of the extracts were analyzed by immunological-based ELISA. As the main result of this research, it was found that extracts of goji berries significantly decreased the cell viability of breast cancer cells and caused these cells to undergo apoptosis-mediated cell death in a dose-and time-dependent manner. Among the tested extracts, methanol extracts showed the highest anticancer activity against MCF-7 and T47D cells (IC 50 =54.06±0.05 µ g/mL and 76.14±0.38 µ g/mL, p<0.01, respectively), while the lowest activity was observed in goji berry extracts prepared with dichloromethane (IC 50 =101.05±0.14 µ g/mL, p<0.05 and 124.10±0.86 µ g/mL, p<0.01, respectively). Moreover, the methanol extracts caused the strongest antiproliferative activity in MCF-7 cells (p<0.05). Regarding the apoptotic cell death potential of the extracts, increased apoptotic cell death was observed in both breast cancer cells, however, apoptotic cell death occurred more strongly in MCF-7 cells than in T47D cells. Consequently, this study suggests that goji berries could be valuable natural sources for the development of herbal formulations against breast cancer. Accordingly, further detailed studies should be conducted to elucidate the molecular signaling pathways and mechanisms of action.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

QbD based approach to RP-HPLC method development and validation of Bupivacaine hydrochloride in bulk and in-house developed nanostructured lipid carriers 基于QbD的反相高效液相色谱法开发和验证盐酸布比卡因散装和自主开发的纳米结构脂质载体

IF 0.8

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.475

Sarfaraz Khan, F. Khan

{"title":"QbD based approach to RP-HPLC method development and validation of Bupivacaine hydrochloride in bulk and in-house developed nanostructured lipid carriers","authors":"Sarfaraz Khan, F. Khan","doi":"10.29228/jrp.475","DOIUrl":"https://doi.org/10.29228/jrp.475","url":null,"abstract":": The present study describes the principles of systematic Quality by design (QbD) approach for the development of RP-HPLC method for the quantification of Bupivacaine hydrochloride (BUP) in bulk and in-house developed nanostructured lipid carriers (NLCs). Initially analytical target profile (ATP), and critical analytical attributes (CAAs) were identified. Primary assessment studies were checked using Plackett-Burman Design. Further optimization studies were performed by applying Box-Behnken design. The Shimadzu C-18 column (250mm x 4.6mm i.d., 5µm particle size) was utilized for reversed-phase chromatographic separation with a mobile phase comprising a mixture of acetonitrile (ACN) and 0.1% ortho phosphoric acid (OPA) (pH 2.04) in 69.45:30.55 (% v/v). The flow rate was 0.805mL/min at a λ max of 214 nm and an injection volume of 12µL. The new developed method was validated according to the guidelines given by International Conference on Harmonization which revealed linearity between 25 to 80µg/ml and r 2 = 0.999. The result of % RSD was 0.38 and 0.44 respectively for high degree of intraday and interday precision. As per the new method the LOD and LOQ is 0.900µg/ml and 2.72µg/ml, respectively. Further the validated method was also applied for the estimation of BUP in NLCs formulation, which showed no interference of any formulation excipients. The studies demonstrated that the new method is rapid, simple, selective, and reproducible for the estimation of pure drug and in-house developed NLCs.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69840002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Origanum minutiflorum O. Schwarz et P. H. Davis essential oil: enzyme inhibitory activities and chemical composition. 荆芥精油:酶抑制活性及化学成分。

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.498

Gülsüm YILDIZ, Betül DEMİRCİ, Halide Edip TEMEL, Neşe KIRIMER

引用次数: 0

Antiepileptic Effect of Musa paradisiaca Stem Juice on Pentylenetetrazole (PTZ)-induced Seizures in Albino Rats 天麻茎汁对戊四唑(PTZ)致白化大鼠癫痫发作的抗癫痫作用

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.398

Sabastine Chinweike UGWUOKE, Valentine Odirachukwumma NWANELO, Martins Obinna OGUGOFOR, Obiora Celestine UGWU, Chioma Assumpta ANOSIKE

引用次数: 1

DRUG DELIVERY SYSTEMS USED FOR BIOLOGICAL PRODUCTS 用于生物制品的药物输送系统

Journal of Research in Pharmacy Pub Date : 2023-01-01 DOI: 10.29228/jrp.501

Ongun Mehmet SAKA

引用次数: 0