Journal of Pharmacy and Pharmacology最新文献_第7页

The potential applications of nanocomposites in 3D-printed drug delivery systems. 纳米复合材料在3d打印给药系统中的潜在应用。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf028

Marwan Algellay, Satyajit D Sarker, Matthew Roberts, Lucy A Bosworth, Touraj Ehtezazi

{"title":"The potential applications of nanocomposites in 3D-printed drug delivery systems.","authors":"Marwan Algellay, Satyajit D Sarker, Matthew Roberts, Lucy A Bosworth, Touraj Ehtezazi","doi":"10.1093/jpp/rgaf028","DOIUrl":"10.1093/jpp/rgaf028","url":null,"abstract":"Additive manufacturing is a renowned technology for producing three-dimensional objects, based on ceramic, metal, and plastic materials for different applications. This review examines and provides a perspective on using nanomaterials along with biopolymeric matrices for 3D printing (3DP) with potential applications in pharmaceutical dosage forms. Many 3DP methods have been developed for the formulation of drug delivery systems, including stereolithography, fused deposition modelling (FDM), selective laser sintering, and bioprinting through droplet- or extrusion-assisted techniques. Polymeric drug-loaded nanocapsules regulated the drug release profiles from 3D-printed tablets with faster drug release from 50% infill tablets. Also, incorporating nanomaterials/micro-ribbons significantly changed the mechanical and flow properties of polymers used in 3DP. For example, the addition of 1% w/w chitosan micro-ribbons to poly-vinyl alcohol powder improved filament mechanical properties for FDM 3DP in terms of flexibility and stiffness, with enhanced disintegration time of 3D-printed oral films. Berberine nanoparticles were integrated into a biodegradable and biocompatible 3D-printed pill, which facilitated sustained drug release and improved gastrointestinal absorption. Furthermore, nanocrystals enhanced the solubility of 3D-printed oral films. In conclusion, nanocomposites improved 3D-printed drug delivery systems in different aspects such as mechanical strength, solubility, and drug release profiles.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"986-1001"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gegen Qinlian Decoction protects kidney in diabetic rats by improving intestinal barrier and regulating intestinal microbiota. 葛根芩连汤通过改善肠道屏障和调节肠道菌群对糖尿病大鼠肾脏的保护作用。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf030

Xinyu Zhang, Qing He, Chenxu Zhang, Zhangxin Ji, Dongmei Yang, Xueyang Wang, Conghui Liu, Chuanqi Zhang, Jingjing Yuan, Na Xu, Jun Chu

{"title":"Gegen Qinlian Decoction protects kidney in diabetic rats by improving intestinal barrier and regulating intestinal microbiota.","authors":"Xinyu Zhang, Qing He, Chenxu Zhang, Zhangxin Ji, Dongmei Yang, Xueyang Wang, Conghui Liu, Chuanqi Zhang, Jingjing Yuan, Na Xu, Jun Chu","doi":"10.1093/jpp/rgaf030","DOIUrl":"10.1093/jpp/rgaf030","url":null,"abstract":"Objectives: To investigate the renoprotective effects of Gegen Qinlian Decoction (GQD) in Diabetes mellitus (DM) rats via the intestinal barrier and microbiota.Methods: GQD was analyzed by UPLC. STZ-induced DM rat models and antibiotic-induced sterile DM rat models were established, and fecal microbiota transplantation was performed in the latter. Renal function, oxidative stress, serum inflammatory factors, and pathological alterations were assessed. Intestinal cells and tight junction were observed by transmission electron microscopy. Inflammatory factors in the colon and tight junction protein expression were evaluated. The gut microbiota and its abundance were assessed by 16sRNA sequencing.Key findings: Four components were determined in the GQD, including puerarin, baicalin, berberine, and liquiritin. After GQD treatment, Scr and BUN were reduced, renal pathological changes were attenuated, intestinal cell swelling was reduced, intestinal tight junctions were improved, and GQD modulated the intestinal microbiota. Furthermore, a fecal bacterial solution containing GQD reduced renal lesions, improved intestinal tight junctions, and regulated intestinal microbiota in DM rats.Conclusions: GQD regulated the intestinal microbiota of DM rats, reduced intestinal inflammation, and repaired the intestinal barrier, thus reducing the burden on the kidneys, and exerting a protective effect on the kidneys of DM rats.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1109-1119"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ending nuclear weapons, before they end us†. 在核武器终结我们之前终结它们。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf026

Kamran Abbasi, Parveen Ali, Virginia Barbour, Marion Birch, Inga Blum, Peter Doherty, Andy Haines, Ira Helfand, Richard Horton, Kati Juva, Jose F Lapena, Robert Mash, Olga Mironova, Arun Mitra, Carlos Monteiro, Elena N Naumova, David Onazi, Tilman Ruff, Peush Sahni, James Tumwine, Carlos Umaña, Paul Yonga, Chris Zielinski

引用次数: 0

Validating the antidiabetic potential of Nakima (Tupistra clarkei Hook.f.), a traditional food from eastern Himalayan region, through network pharmacology and in vivo experimentation. 通过网络药理学和体内实验验证喜马拉雅东部地区传统食物Nakima （Tupistra clarkei Hook.f.）的抗糖尿病潜力。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf014

Sutapa Datta, Soumita Bhattacharjee, Supriyo Ghosh, Amlan Jyoti Ghosh, Tilak Saha, Arnab Sen

{"title":"Validating the antidiabetic potential of Nakima (Tupistra clarkei Hook.f.), a traditional food from eastern Himalayan region, through network pharmacology and in vivo experimentation.","authors":"Sutapa Datta, Soumita Bhattacharjee, Supriyo Ghosh, Amlan Jyoti Ghosh, Tilak Saha, Arnab Sen","doi":"10.1093/jpp/rgaf014","DOIUrl":"10.1093/jpp/rgaf014","url":null,"abstract":"Objective: To explore and understand the antidiabetic activity of Tupistra clarkei Hook.f. inflorescence, providing a scientific explanation to the ethnomedicinal properties.Methods: The constituents of the plant were determined through GC-MS analysis, which were used for target prediction and network pharmacology to understand how the plant regulates hyperglycaemia and other diabetes complications. These properties were validated in vivo along with further assessment of the antioxidant potential of the plant, both in vitro and in vivo.Key findings: The plant showed good phenol-flavonoid content, and antioxidant potential both in vitro and in vivo. GC-MS analysis identified 24 constituents of the plant. In silico analysis showed their ability to target 166 proteins that are associated with pathways in controlling hyperglycaemia and other diabetic consequences, protection of pancreatic tissue, insulin secretion, and insulin resistance. This was reflected in the in vivo experiment where T. clarkei showed ability to reduce FBG, LDL-C, VLDL-C levels, improve the levels of HDL-C, and also facilitate reversal of damage in pancreatic islets.Conclusion: Our study validated the antidiabetic potential Tupistra clarkei inflorescence in the in silico and in vivo assessment, and has proved to have good antioxidant activity and potential against diabetes. However, further clinical trials are essential.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1059-1074"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Full spectrum cannabis oil combined with omega-3 fish oil for neuropathic pain management: a novel therapeutic approach. 全谱大麻油与omega-3鱼油联合用于神经性疼痛管理：一种新的治疗方法。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf027

Cristina F Elorriaga, María E Olivera, Hugo Gongora Jara, Carlos H Laino

{"title":"Full spectrum cannabis oil combined with omega-3 fish oil for neuropathic pain management: a novel therapeutic approach.","authors":"Cristina F Elorriaga, María E Olivera, Hugo Gongora Jara, Carlos H Laino","doi":"10.1093/jpp/rgaf027","DOIUrl":"10.1093/jpp/rgaf027","url":null,"abstract":"Objectives: Current pharmacological treatments for neuropathic pain have limited efficacy and may cause undesirable side effects. Cannabidiol (CBD)-enriched cannabis oil and omega-3 fatty acids (ω-3) have emerged as potential therapeutic options due to their analgesic and anti-inflammatory properties. This study aimed to assess the antinociceptive effects of combining CBD-enriched cannabis oil and ω-3 in rat models of acute and neuropathic pain.Methods: Using the hot plate test for acute pain and the chronic constriction injury (CCI) model for neuropathic pain, thermal and mechanical hypersensitivity were evaluated. Additionally, walking track analysis and the rotarod test assessed functional recovery of the sciatic nerve. Beyond that, the histological analysis of sciatic nerves exposed the neuropathological findings of the treatments.Key findings: The combined treatment of CBD-enriched cannabis oil and ω-3 effectively prevented thermal and mechanical hypersensitivity, while also improving motor impairment-induced peripheral neuropathy. Finally, combination treatment showed a protective effect against degeneration resulting from CCI.Conclusions: These findings underscore the potential of CBD-enriched cannabis oil and ω-3 as a novel therapeutic approach for neuropathic pain management, offering promising implications for future research and clinical practice.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1097-1108"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gold nanoparticle/silk fibroin-based nanofiber enhances skin regeneration. 金纳米粒子/丝素纳米纤维增强皮肤再生。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf025

Ozan Ozcan, Elif Tufan, Aleyna Muhan, Esin Ak, Göksel Şener, Tugba Tunali-Akbay

{"title":"Gold nanoparticle/silk fibroin-based nanofiber enhances skin regeneration.","authors":"Ozan Ozcan, Elif Tufan, Aleyna Muhan, Esin Ak, Göksel Şener, Tugba Tunali-Akbay","doi":"10.1093/jpp/rgaf025","DOIUrl":"10.1093/jpp/rgaf025","url":null,"abstract":"Objectives: The aim of this study was to determine the wound-healing potential of gold nanoparticles and silk fibroin-based nanofiber produced by green chemistry.Methods: The electrospinning method was used to prepare the nanofiber. Twenty rats were exposed to a 7-day treatment period and another 20 rats were exposed to a 21-day treatment period. Rat groups were control, silver, silk fibroin, and silk + gold nanoparticle groups for each period. The effect of the gold nanoparticle/silk fibroin-based nanofiber was examined in skin samples by using biochemical and histological analysis. In biochemical analysis, skin oxidant and antioxidant parameters were determined.Key findings: Parameters indicating skin damage returned to their previous levels 7 and 21 days after the wound formation using gold nanoparticle/silk fibroin-based nanofiber. Gold nanoparticle/silk fibroin-based nanofiber initiated hair follicle formation at the wound site and accelerated the re-epithelialization process.Conclusions: It was found that the nanofiber prepared by adding gold nanoparticles to silk fibroin had better wound-healing properties than silk fibroin nanofibers without gold nanoparticles.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1085-1096"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating reversible inhibitory drug-drug interactions: enzyme kinetics, and in vitro-to-in vivo scaling models. 评估可逆的抑制性药物-药物相互作用：酶动力学和体外-体内缩放模型。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf029

Qingchen Zhang, David J Greenblatt, Philip W Melchert, John S Markowitz

{"title":"Evaluating reversible inhibitory drug-drug interactions: enzyme kinetics, and in vitro-to-in vivo scaling models.","authors":"Qingchen Zhang, David J Greenblatt, Philip W Melchert, John S Markowitz","doi":"10.1093/jpp/rgaf029","DOIUrl":"10.1093/jpp/rgaf029","url":null,"abstract":"Background: Polypharmacy is common in clinical practice, making the consideration of potential drug-drug interactions (DDIs) an important factor in clinical therapeutics. In vitro methods are applied for screening and anticipating possible DDIs, with mathematical models playing a key role in evaluating inhibitor potency and scaling pharmacokinetic parameters from in vitro data. Despite extensive research on this topic, varying assumptions and experimental settings across studies have led to inconsistency among models, with the possible consequence of misapplication of enzyme kinetic models and scaling procedures, and misdirection in DDI evaluation and predictions.Methods: This study reviews and summarizes common enzyme kinetic models used to analyse substrate-enzyme-inhibitor interactions across six different mechanisms of inhibition, and derives the corresponding in vitro to in vivo scaling model for use in connecting to clinical DDI studies.Results: A single operational equation was developed, along with a method for determining the inhibition mechanism and the connection to anticipation of in vivo pharmacokinetics.Conclusion: Analysis based on the equation shows that, for inhibitors with the same inhibition constant (Ki), competitive inhibitors will pose a higher potential for DDIs compared to non-competitive inhibitors, while complete inhibitors will result in a higher potential for DDI than partial inhibitors.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1002-1010"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the in vitro, in vivo anti-inflammatory potential of Ocimum basilicum and in silico analysis of its phytocompounds targeting COXs proteins. 揭示basilicum的体外和体内抗炎潜力，并对其靶向COXs蛋白的植物化合物进行硅分析。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf032

Nimrah Zafar, Azhar Rafique, Shabana Naz, Muhammad Muzammil Nazir, Asma Ashraf

{"title":"Unveiling the in vitro, in vivo anti-inflammatory potential of Ocimum basilicum and in silico analysis of its phytocompounds targeting COXs proteins.","authors":"Nimrah Zafar, Azhar Rafique, Shabana Naz, Muhammad Muzammil Nazir, Asma Ashraf","doi":"10.1093/jpp/rgaf032","DOIUrl":"10.1093/jpp/rgaf032","url":null,"abstract":"Objectives: This study aimed to evaluate the anti-inflammatory potential of ethanolic extract of Ocimum basilicum seeds (EEOBS) through in vitro, in vivo, and in silico approaches.Methods: The in vivo anti-inflammatory activity of EEOBS was assessed using a carrageenan-induced paw edema model in Swiss albino mice, where paw thickness was measured at 1, 2, 3, 4, and 5 hours post-treatment. The in vitro anti-inflammatory potential was evaluated using a bovine serum albumin (BSA) denaturation assay at varying concentrations of EEOBS (50, 100, 250, 500, and 1000 μg/ml).Key findings: Gas chromatography-mass spectrometry (GC-MS) analysis of EEOBS revealed the presence of several bioactive phytochemicals, with 9,12,15-Octadecatrienoic acid (47.27%) and hexadecanoic acid (13.45%) as the major constituents. Histopathological analysis of mice paws showed significant restoration of the keratin and epithelium layers in treated groups compared to the control. Molecular docking analysis identified linoleic acid and 12-Z-octadecatrienoic acid as the most promising compounds, demonstrating higher binding affinity than the standard inhibitor for both cyclooxygenase proteins (COX-1: PDB ID 1EQG and COX-2: PDB ID 1CX2). Additionally, n-octadecanoic acid exhibited superior binding with COX-2 (1CX2).Conclusion: The in vitro, in vivo, and in silico findings suggest that EEOBS possesses significant anti-inflammatory potential, indicating its suitability for targeted anti-inflammatory therapies. However, further clinical trials are required to validate its therapeutic efficacy.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1133-1147"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of quetiapine-loaded microneedles-based transdermal patches for improved drug delivery. 奎硫平微针透皮贴剂的开发与特性研究。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf013

Maryam Itbar, Muhammad Imran Khan, Muhammad Furqan Akhtar, Zulcaif Ahmad, Muhammad Farhan Sohail, Asadullah Madni, Alia Erum, Aslam Khan, Ahsan Ali, Muhammad Naeem Qaisar

{"title":"Development and characterization of quetiapine-loaded microneedles-based transdermal patches for improved drug delivery.","authors":"Maryam Itbar, Muhammad Imran Khan, Muhammad Furqan Akhtar, Zulcaif Ahmad, Muhammad Farhan Sohail, Asadullah Madni, Alia Erum, Aslam Khan, Ahsan Ali, Muhammad Naeem Qaisar","doi":"10.1093/jpp/rgaf013","DOIUrl":"10.1093/jpp/rgaf013","url":null,"abstract":"Objectives: This study was executed to prepare and characterize quetiapine (antipsychotic drug)-loaded microneedles-based transdermal patch for improved drug delivery.Methods: This study was executed to develop microneedles-based transdermal patches (MNS) for quetiapine delivery. Eight MNS patches loaded with quetiapine (MNS1-MNS8) were fabricated using varying concentrations of sodium alginate and carboxymethyl cellulose. First four MNS patches (MNS1, MNS2, MNS3, and MNS4) were prepared by keeping sodium alginate concentration constant (6%) and increasing CMC concentration from 3% to 6%, whereas MNS5, MNS6, MNS7, and MNS8 were developed using sodium alginate to CMC concentrations 7:3, 7:4, 8:3, and 8:4, respectively. Solvent casting technique was opted for preparation of MNS patches. MNS were characterized for thickness, folding endurance, insertion capacity, drug content, morphology, and ex-vivo permeation profile using Wistar rat skin.Key findings: FTIR studies revealed the compatibility of quetiapine with formulation composites. Thickness and folding endurance was ranged in between 0.53-0.55 mm and 25-264, respectively. SEM of optimized patch showed sharp pointed needles. Ex-vivo permeation studies showed percent drug release of 84.34% from MNS1 after 48 h.Conclusions: The overall findings of study proposed that the quetiapine-loaded MNS patches hold promise for the improved transdermal delivery of quetiapine.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1042-1058"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ginsenoside Rb1 inhibits chronic stress-induced colorectal cancer via regulating glycolysis and β2-AR/CREB1 signaling pathway. 人参皂苷Rb1通过调节糖酵解和β2-AR/CREB1信号通路抑制慢性应激性结直肠癌。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf031

Wang Yao, Dong-Ming Hua, Wen-Kai Wang, Zhao-Zhou Zhang, Yun-Feng Guan, Yan Wang

{"title":"Ginsenoside Rb1 inhibits chronic stress-induced colorectal cancer via regulating glycolysis and β2-AR/CREB1 signaling pathway.","authors":"Wang Yao, Dong-Ming Hua, Wen-Kai Wang, Zhao-Zhou Zhang, Yun-Feng Guan, Yan Wang","doi":"10.1093/jpp/rgaf031","DOIUrl":"10.1093/jpp/rgaf031","url":null,"abstract":"Objective: To investigate the mechanism of ginsenoside Rb1 (G-Rb1) against colorectal cancer under chronic stress.Methods: A chronic restraint stress (CRS) model and a colorectal cancer (CRC) subcutaneous xenograft model were established. Western blot analysis quantified β2-adrenergic receptor (β2-AR), cAMP response element-binding protein 1 (CREB1), and p-CREB1 expression. Additionally, glycolytic enzymes GLUT1, HK2, and PFKP were analyzed via Western blot and RT-qPCR, with glucose uptake, lactate, ATP, and stress hormone levels assessed by flow cytometry, kits, and ELISA.Key findings: Compared to the control group, the stress group exhibited increased tumor volume and mass, along with elevated expression of β2-AR, p-CREB1, and upregulated expression levels of GLUT1, HK2, and PFKP. Additionally, glucose, lactate, and epinephrine levels were higher in the stress group. In comparison to the stress group, G-Rb1 treatment demonstrated reduced tumor volume and mass, decreased p-CREB1 expression, as well as downregulated protein and mRNA levels of GLUT1, HK2, and PFKP. Glucose, lactate, and epinephrine levels also showed a reduction in the G-Rb1-treated groups.Conclusions: G-Rb1 suppresses the growth of colorectal cancer under chronic stress, potentially through downregulation of the β2-AR/CREB1 signaling pathway, thereby reducing glycolytic activity in colorectal cancer under chronic stress.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1120-1132"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0