Brazilian red propolis synergistically with imipenem modulates immunological parameters and the bactericidal activity of human monocytes against methicillin-resistant Staphylococcus aureus (MRSA).

IF 2.8 4区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of Pharmacy and Pharmacology Pub Date : 2024-10-25 DOI:10.1093/jpp/rgae135

Nicolas Ripari, Mariana da Silva Honorio, Arthur Alves Sartori, Larissa Ragozo Cardoso de Oliveira, Jairo Kenupp Bastos, José Maurício Sforcin

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Abstract

Objectives: Propolis is a bee product found all over the globe and has a well-known antibacterial activity. Previous findings of our group revealed that the combination of Brazilian red propolis (BRP) with a lower concentration of imipenem (IPM) exerted a bactericidal action against methicillin-resistant Staphylococcus aureus (MRSA) in vitro. Here, we aimed at investigating the effects of BRP in combination or not with IPM on human monocytes to assess a possible immunomodulatory action.

Methods: Monocyte metabolic activity was analysed by MTT assay, cytokine production (TNF-α, IL-1β, IL-6, IL-8, and IL-10) by ELISA, and the expression of cell markers (TLR-2, TLR-4, HLA-DR, and CD80) by flow cytometry. The bactericidal activity of monocytes over MRSA was determined by colony-forming units' count.

Key findings: BRP alone or in combination with IPM exerted no cytotoxic effects on monocytes. BRP downregulated TLR-2 expression and inhibited TNF-α, IL-1β, and IL-6 production, while BRP + IPM stimulated these parameters. BPR alone or in combination increased the bactericidal activity similarly to LPS-activated monocytes.

Conclusions: Data indicated the potential of BRP as an anti-inflammatory agent increasing the bactericidal activity of monocytes against MRSA. The combination of BRP + IPM exhibited a stimulatory profile that may be potentially useful in treating patients with MRSA infection.

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巴西红蜂胶与亚胺培南协同调节人体单核细胞对耐甲氧西林金黄色葡萄球菌（MRSA）的免疫学参数和杀菌活性。

目的：蜂胶是一种遍布全球的蜂产品，具有众所周知的抗菌活性。我们小组以前的研究结果表明，巴西红蜂胶（BRP）与低浓度亚胺培南（IPM）在体外对耐甲氧西林金黄色葡萄球菌（MRSA）具有杀菌作用。在此，我们旨在研究 BRP 与 IPM 结合或不结合对人类单核细胞的影响，以评估可能的免疫调节作用：方法：通过 MTT 试验分析单核细胞的代谢活性，通过 ELISA 分析细胞因子的产生（TNF-α、IL-1β、IL-6、IL-8 和 IL-10），通过流式细胞术分析细胞标志物的表达（TLR-2、TLR-4、HLA-DR 和 CD80）。通过菌落形成单位计数确定单核细胞对 MRSA 的杀菌活性：主要发现：BRP单独或与IPM联合使用对单核细胞无细胞毒性作用。BRP 下调了 TLR-2 的表达，抑制了 TNF-α、IL-1β 和 IL-6 的产生，而 BRP + IPM 则刺激了这些参数的产生。与 LPS 激活的单核细胞相似，BPR 单独或联合使用都能提高杀菌活性：数据表明，BRP 可作为一种抗炎剂提高单核细胞对 MRSA 的杀菌活性。BRP+IPM的组合具有刺激作用，可能有助于治疗MRSA感染患者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Pharmacy and Pharmacology 医学-药学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.