Journal of Pharmacy and Pharmacology最新文献_第8页

Evaluating reversible inhibitory drug-drug interactions: enzyme kinetics, and in vitro-to-in vivo scaling models. 评估可逆的抑制性药物-药物相互作用：酶动力学和体外-体内缩放模型。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf029

Qingchen Zhang, David J Greenblatt, Philip W Melchert, John S Markowitz

{"title":"Evaluating reversible inhibitory drug-drug interactions: enzyme kinetics, and in vitro-to-in vivo scaling models.","authors":"Qingchen Zhang, David J Greenblatt, Philip W Melchert, John S Markowitz","doi":"10.1093/jpp/rgaf029","DOIUrl":"10.1093/jpp/rgaf029","url":null,"abstract":"Background: Polypharmacy is common in clinical practice, making the consideration of potential drug-drug interactions (DDIs) an important factor in clinical therapeutics. In vitro methods are applied for screening and anticipating possible DDIs, with mathematical models playing a key role in evaluating inhibitor potency and scaling pharmacokinetic parameters from in vitro data. Despite extensive research on this topic, varying assumptions and experimental settings across studies have led to inconsistency among models, with the possible consequence of misapplication of enzyme kinetic models and scaling procedures, and misdirection in DDI evaluation and predictions.Methods: This study reviews and summarizes common enzyme kinetic models used to analyse substrate-enzyme-inhibitor interactions across six different mechanisms of inhibition, and derives the corresponding in vitro to in vivo scaling model for use in connecting to clinical DDI studies.Results: A single operational equation was developed, along with a method for determining the inhibition mechanism and the connection to anticipation of in vivo pharmacokinetics.Conclusion: Analysis based on the equation shows that, for inhibitors with the same inhibition constant (Ki), competitive inhibitors will pose a higher potential for DDIs compared to non-competitive inhibitors, while complete inhibitors will result in a higher potential for DDI than partial inhibitors.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1002-1010"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the in vitro, in vivo anti-inflammatory potential of Ocimum basilicum and in silico analysis of its phytocompounds targeting COXs proteins. 揭示basilicum的体外和体内抗炎潜力，并对其靶向COXs蛋白的植物化合物进行硅分析。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf032

Nimrah Zafar, Azhar Rafique, Shabana Naz, Muhammad Muzammil Nazir, Asma Ashraf

{"title":"Unveiling the in vitro, in vivo anti-inflammatory potential of Ocimum basilicum and in silico analysis of its phytocompounds targeting COXs proteins.","authors":"Nimrah Zafar, Azhar Rafique, Shabana Naz, Muhammad Muzammil Nazir, Asma Ashraf","doi":"10.1093/jpp/rgaf032","DOIUrl":"10.1093/jpp/rgaf032","url":null,"abstract":"Objectives: This study aimed to evaluate the anti-inflammatory potential of ethanolic extract of Ocimum basilicum seeds (EEOBS) through in vitro, in vivo, and in silico approaches.Methods: The in vivo anti-inflammatory activity of EEOBS was assessed using a carrageenan-induced paw edema model in Swiss albino mice, where paw thickness was measured at 1, 2, 3, 4, and 5 hours post-treatment. The in vitro anti-inflammatory potential was evaluated using a bovine serum albumin (BSA) denaturation assay at varying concentrations of EEOBS (50, 100, 250, 500, and 1000 μg/ml).Key findings: Gas chromatography-mass spectrometry (GC-MS) analysis of EEOBS revealed the presence of several bioactive phytochemicals, with 9,12,15-Octadecatrienoic acid (47.27%) and hexadecanoic acid (13.45%) as the major constituents. Histopathological analysis of mice paws showed significant restoration of the keratin and epithelium layers in treated groups compared to the control. Molecular docking analysis identified linoleic acid and 12-Z-octadecatrienoic acid as the most promising compounds, demonstrating higher binding affinity than the standard inhibitor for both cyclooxygenase proteins (COX-1: PDB ID 1EQG and COX-2: PDB ID 1CX2). Additionally, n-octadecanoic acid exhibited superior binding with COX-2 (1CX2).Conclusion: The in vitro, in vivo, and in silico findings suggest that EEOBS possesses significant anti-inflammatory potential, indicating its suitability for targeted anti-inflammatory therapies. However, further clinical trials are required to validate its therapeutic efficacy.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1133-1147"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of quetiapine-loaded microneedles-based transdermal patches for improved drug delivery. 奎硫平微针透皮贴剂的开发与特性研究。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf013

Maryam Itbar, Muhammad Imran Khan, Muhammad Furqan Akhtar, Zulcaif Ahmad, Muhammad Farhan Sohail, Asadullah Madni, Alia Erum, Aslam Khan, Ahsan Ali, Muhammad Naeem Qaisar

{"title":"Development and characterization of quetiapine-loaded microneedles-based transdermal patches for improved drug delivery.","authors":"Maryam Itbar, Muhammad Imran Khan, Muhammad Furqan Akhtar, Zulcaif Ahmad, Muhammad Farhan Sohail, Asadullah Madni, Alia Erum, Aslam Khan, Ahsan Ali, Muhammad Naeem Qaisar","doi":"10.1093/jpp/rgaf013","DOIUrl":"10.1093/jpp/rgaf013","url":null,"abstract":"Objectives: This study was executed to prepare and characterize quetiapine (antipsychotic drug)-loaded microneedles-based transdermal patch for improved drug delivery.Methods: This study was executed to develop microneedles-based transdermal patches (MNS) for quetiapine delivery. Eight MNS patches loaded with quetiapine (MNS1-MNS8) were fabricated using varying concentrations of sodium alginate and carboxymethyl cellulose. First four MNS patches (MNS1, MNS2, MNS3, and MNS4) were prepared by keeping sodium alginate concentration constant (6%) and increasing CMC concentration from 3% to 6%, whereas MNS5, MNS6, MNS7, and MNS8 were developed using sodium alginate to CMC concentrations 7:3, 7:4, 8:3, and 8:4, respectively. Solvent casting technique was opted for preparation of MNS patches. MNS were characterized for thickness, folding endurance, insertion capacity, drug content, morphology, and ex-vivo permeation profile using Wistar rat skin.Key findings: FTIR studies revealed the compatibility of quetiapine with formulation composites. Thickness and folding endurance was ranged in between 0.53-0.55 mm and 25-264, respectively. SEM of optimized patch showed sharp pointed needles. Ex-vivo permeation studies showed percent drug release of 84.34% from MNS1 after 48 h.Conclusions: The overall findings of study proposed that the quetiapine-loaded MNS patches hold promise for the improved transdermal delivery of quetiapine.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1042-1058"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ginsenoside Rb1 inhibits chronic stress-induced colorectal cancer via regulating glycolysis and β2-AR/CREB1 signaling pathway. 人参皂苷Rb1通过调节糖酵解和β2-AR/CREB1信号通路抑制慢性应激性结直肠癌。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf031

Wang Yao, Dong-Ming Hua, Wen-Kai Wang, Zhao-Zhou Zhang, Yun-Feng Guan, Yan Wang

{"title":"Ginsenoside Rb1 inhibits chronic stress-induced colorectal cancer via regulating glycolysis and β2-AR/CREB1 signaling pathway.","authors":"Wang Yao, Dong-Ming Hua, Wen-Kai Wang, Zhao-Zhou Zhang, Yun-Feng Guan, Yan Wang","doi":"10.1093/jpp/rgaf031","DOIUrl":"10.1093/jpp/rgaf031","url":null,"abstract":"Objective: To investigate the mechanism of ginsenoside Rb1 (G-Rb1) against colorectal cancer under chronic stress.Methods: A chronic restraint stress (CRS) model and a colorectal cancer (CRC) subcutaneous xenograft model were established. Western blot analysis quantified β2-adrenergic receptor (β2-AR), cAMP response element-binding protein 1 (CREB1), and p-CREB1 expression. Additionally, glycolytic enzymes GLUT1, HK2, and PFKP were analyzed via Western blot and RT-qPCR, with glucose uptake, lactate, ATP, and stress hormone levels assessed by flow cytometry, kits, and ELISA.Key findings: Compared to the control group, the stress group exhibited increased tumor volume and mass, along with elevated expression of β2-AR, p-CREB1, and upregulated expression levels of GLUT1, HK2, and PFKP. Additionally, glucose, lactate, and epinephrine levels were higher in the stress group. In comparison to the stress group, G-Rb1 treatment demonstrated reduced tumor volume and mass, decreased p-CREB1 expression, as well as downregulated protein and mRNA levels of GLUT1, HK2, and PFKP. Glucose, lactate, and epinephrine levels also showed a reduction in the G-Rb1-treated groups.Conclusions: G-Rb1 suppresses the growth of colorectal cancer under chronic stress, potentially through downregulation of the β2-AR/CREB1 signaling pathway, thereby reducing glycolytic activity in colorectal cancer under chronic stress.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1120-1132"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cannabidiol and microRNAs: shared cellular targets and new insights for developing anti-seizure modalities. 大麻二酚和microrna：共享细胞靶点和开发抗癫痫模式的新见解。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf039

Arya Afrooghe, Elham Ahmadi, Maryam Shayan, Mohammad Amin Dabbagh Ohadi, Amir Hossein Behnoush, Ahmad Reza Dehpour

{"title":"Cannabidiol and microRNAs: shared cellular targets and new insights for developing anti-seizure modalities.","authors":"Arya Afrooghe, Elham Ahmadi, Maryam Shayan, Mohammad Amin Dabbagh Ohadi, Amir Hossein Behnoush, Ahmad Reza Dehpour","doi":"10.1093/jpp/rgaf039","DOIUrl":"10.1093/jpp/rgaf039","url":null,"abstract":"Cannabidiol (CBD) and 9-tetrahydrocannabinol (THC) are the two main components of cannabis that provide its therapeutic benefits. CBD has been extensively studied for its role in reducing seizures, among its many other uses. While the exact mechanisms by which CBD works to relieve seizures have not yet been fully determined, it is evident that CBD effectively diminishes seizure activity and is now being used as an approved treatment for severe forms of non-responsive epilepsy. As essential components of several biological processes, microRNAs (miRNAs) are crucial for achieving optimal cellular functioning. During the past few years, there has been an increasing interest in elucidating the functional significance of microRNAs in epilepsy. Yet, there is still considerable ambiguity regarding the precise mechanisms by which miRNAs are involved in seizure disorders and also their direct interaction with CBD. Herein, we seek to present an overview of the varying mechanisms by which CBD offers therapeutic benefits concerning seizures. Accordingly, we highlighted the shared molecular targets between microRNAs and CBD in alleviating seizure symptoms to shed light on the ways in which new therapeutic and diagnostic modalities could be shaped in the future.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1011-1022"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of chemical and blood transition components of Xipayi Maizibizi Oral Liquid and its excitatory purine regulation-mechanism study in treating overactive bladder. 喜帕依麦子滋口服液化学成分、血运成分分析及治疗膀胱过动症的兴奋性嘌呤调节机制研究。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf011

Menglu Wang, Yang Yang, Jiamei Xie, Yuhang Du, Yige Zhao, Yongcheng An, Ziyi Shan, Changhao He, Wanxin Fu, Yan Huang, Huilin Zhang, Baosheng Zhao

{"title":"Analysis of chemical and blood transition components of Xipayi Maizibizi Oral Liquid and its excitatory purine regulation-mechanism study in treating overactive bladder.","authors":"Menglu Wang, Yang Yang, Jiamei Xie, Yuhang Du, Yige Zhao, Yongcheng An, Ziyi Shan, Changhao He, Wanxin Fu, Yan Huang, Huilin Zhang, Baosheng Zhao","doi":"10.1093/jpp/rgaf011","DOIUrl":"10.1093/jpp/rgaf011","url":null,"abstract":"Objective: To explore the chemical components and blood transition components of Xipayi Maizibizi Oral Liquid (XP) as well as the efficacy and mechanism of XP in the treatment of overactive bladder (OAB).Methods: The chemical components and blood transition components were analysed. Rats were undergone bladder outlet obstruction surgery and divided into five groups. The study observed the general conditions, urodynamic argument and the bladder histopathological changes. The expression levels of ATP and P2X3 were evaluated.Results: One hundred and eighty-six chemical components were identified in XP, including 26 phenolic acids, 37 flavonoids, 7 quinones, 11 lignans and coumarins, 8 tannins, 9 alkaloids, 37 terpenoids, 32 steroids, and 19 other components. One hundred and twenty-eight components of XP were found in the blood, including 23 phenolic acids, 22 flavonoids, 3 quinones, 8 lignans and coumarins, 7 tannins, 7 alkaloids, 21 terpenoids, 20 steroids, and 17 other components. XP demonstrated effective treatment of OAB and downregulated the expression of ATP and P2X3.Conclusion: The characterization of the chemical composition and blood transition components of XP provided a foundation for further pharmacodynamic material basis and quality control of XP. XP could enhance the pathological status of rats with OAB by regulating ATP/P2X3.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1023-1041"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction of: Potential therapeutic efficacy of pachymic acid in chronic kidney disease induced in rats: role of Wnt/β-catenin/renin-angiotensin axis. 厚青酸对大鼠慢性肾病的潜在治疗作用：Wnt/β-连环蛋白/肾素-血管紧张素轴的作用。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-28 DOI: 10.1093/jpp/rgaf061

引用次数: 0

Mechanisms and potential roles of active ingredients of traditional Chinese medicine in the treatment of chronic obstructive pulmonary disease. 中药有效成分治疗慢性阻塞性肺疾病的机制及潜在作用。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-01 DOI: 10.1093/jpp/rgaf018

Xilin Wu, Yonghu Chen, Hanyu Zhang, Jiamin Wang, Chenchen Tian, Zhe Jiang, Xuezheng Li

{"title":"Mechanisms and potential roles of active ingredients of traditional Chinese medicine in the treatment of chronic obstructive pulmonary disease.","authors":"Xilin Wu, Yonghu Chen, Hanyu Zhang, Jiamin Wang, Chenchen Tian, Zhe Jiang, Xuezheng Li","doi":"10.1093/jpp/rgaf018","DOIUrl":"10.1093/jpp/rgaf018","url":null,"abstract":"Objectives: Chronic obstructive pulmonary disease (COPD) is a respiratory condition with high rates of morbidity and mortality. Recent studies have shown that the increasing research on Traditional Chinese Medicine (TCM) also plays an important role in COPD. The purpose of this review is to categorize TCM and its active ingredients and to summarize their pharmacological effects.Methods: Articles published up to December 2024 were searched through PubMed, X-MOL, and the China National Knowledge Infrastructure. The keywords included TCM and its combination with COPD, pharmacologic activity, anti-inflammatory effects, pharmacology, as well as in vivo and in vitro studies.Key findings: Thus far, we have summarized the progress of research on the mechanisms of action of TCM and its active ingredients, such as flavonoids, terpenoids, and phenols, in the treatment of COPD. These mechanisms encompass the reduction of inflammatory responses and lung injury, regulation of the oxidation-antioxidation balance, and modulation of cellular apoptosis and aging, among other effects.Conclusion: TCM and its active ingredients demonstrate strong anti-COPD properties. This provides a reference for accelerating the development of herbal components for the treatment of COPD and for exploring new potential multi-target therapeutic mechanisms. This will mitigate the geographical limitations of using TCM and enhance its application in future management strategies.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"866-883"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shaoyao Gancao decoction, an Ancient Classical Prescription: a review on its chemical composition, pharmacology, pharmacokinetics, clinical applications, and toxicology. 古代经典处方芍药甘草汤的化学成分、药理学、药代动力学、临床应用及毒理学研究综述。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-01 DOI: 10.1093/jpp/rgaf017

Zhang Facheng, Qiu Rongli, Zhang Li, Wu Baoxiang, Yu Sheng, Shan Mingqiu

{"title":"Shaoyao Gancao decoction, an Ancient Classical Prescription: a review on its chemical composition, pharmacology, pharmacokinetics, clinical applications, and toxicology.","authors":"Zhang Facheng, Qiu Rongli, Zhang Li, Wu Baoxiang, Yu Sheng, Shan Mingqiu","doi":"10.1093/jpp/rgaf017","DOIUrl":"10.1093/jpp/rgaf017","url":null,"abstract":"Objectives: Shaoyao Gancao decoction (SGD) is a famous Ancient Classical Prescription (ACP) from \"Treatise on Febrile Diseases.\" It has been clinically used for spasm- and pain-related disorders induced by insufficiency of Qi and blood and malnutrition of tendons and vessels for thousands of years. To expand comprehensive understanding and to highlight the importance of more effective utilization, this study aimed to provide a comprehensive review of SGD covering multiple research fields.Methods: Some databases, including PubMed, Web of Science, Google Scholar, and China National Knowledge Infrastructure, were used to collect the related information with \"Shaoyao Gancao decoction\" and similar ones as the keywords.Key findings: Phytochemical researches revealed that flavonoids and monoterpenoids were the predominant components in SGD. It was documented that SGD had demonstrated a variety of effects, such as analgesic and anti-inflammatory activity, neuroprotection, antispasmodic activity, gastrointestinal protection, hepatoprotection, anti-asthma activity, and effects on gynecological diseases. As for its toxicology, pseudoaldosteronism occasionally occurred and 18β-glycyrrhetyl-3-O-sulfate was believed to be a causative agent.Conclusions: As a whole, many valuable achievements have been made, exhibiting great attraction and potential of SGD as a famous ACP. This review is also expected to facilitate SGD application and research in the future.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"845-865"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Griffonia simplicifolia seeds extract rich in 5-hydroxy-L-tryptophan reduces infection and inflammation in a mouse model of vulvovaginal candidiasis. 富5-羟基- l -色氨酸的栀子提取物可降低外阴阴道念珠菌病小鼠模型的感染和炎症。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-01 DOI: 10.1093/jpp/rgaf024

Matteo Puccetti, Alessandro Di Michele, Cinzia Pagano, Elisa Pecorari, Alice Coletti, Leonardo Tensi, Marilena Pariano, Rita Pecorari, Maurizio Ricci, Luana Perioli

{"title":"Griffonia simplicifolia seeds extract rich in 5-hydroxy-L-tryptophan reduces infection and inflammation in a mouse model of vulvovaginal candidiasis.","authors":"Matteo Puccetti, Alessandro Di Michele, Cinzia Pagano, Elisa Pecorari, Alice Coletti, Leonardo Tensi, Marilena Pariano, Rita Pecorari, Maurizio Ricci, Luana Perioli","doi":"10.1093/jpp/rgaf024","DOIUrl":"10.1093/jpp/rgaf024","url":null,"abstract":"Background: Griffonia simplicifolia seeds extracts, generally prepared by maceration, are most commonly used in food supplements intended for mental well-being promotion due to the high content of 5-hydroxy-L-tryptophan (5-HTP), direct serotonin precursor. As 5-HTP is involved in many different biochemical pathways, other applications of G. simplicifolia seed extracts were investigated. Considering the well-known serotonin immunomodulatory activity, the object of this study was to optimize 5-HTP yield by a new extraction procedure, exploit its immunomodulatory activity in a preclinical model of vulvovaginal candidiasis (VVC) for its responsiveness to immunomodulation, and formulate the extract in a suitable vaginal formulation.Methods: High-power ultrasonic technique (HPU) was used for the first time to obtain an extract from Griffonia seeds (GSE-HPU). The amount of 5-HTP was measured by UHPLC-MS/MS. The extract was assessed in C57BL/6 mice model intravaginally infected with C. albicans.Results: The results showed that the new extraction procedure greatly increased 5-HTP yield (82.8% w/w of the extract weight); formulated in \"tablets for vaginal solution\" properly characterized, the GSE-HPU showed a remarkable protective activity in VVC as reduces inflammation, tissue pathology, and fungal growth.Conclusions: The protective efficacy of GSE-HPU in VVC suggests its potential use in vaginal infections and associated inflammatory pathology.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"933-943"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0