Journal of Pharmacy and Pharmacology最新文献_第8页

Cannabidiol and microRNAs: shared cellular targets and new insights for developing anti-seizure modalities. 大麻二酚和microrna：共享细胞靶点和开发抗癫痫模式的新见解。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf039

Arya Afrooghe, Elham Ahmadi, Maryam Shayan, Mohammad Amin Dabbagh Ohadi, Amir Hossein Behnoush, Ahmad Reza Dehpour

{"title":"Cannabidiol and microRNAs: shared cellular targets and new insights for developing anti-seizure modalities.","authors":"Arya Afrooghe, Elham Ahmadi, Maryam Shayan, Mohammad Amin Dabbagh Ohadi, Amir Hossein Behnoush, Ahmad Reza Dehpour","doi":"10.1093/jpp/rgaf039","DOIUrl":"10.1093/jpp/rgaf039","url":null,"abstract":"Cannabidiol (CBD) and 9-tetrahydrocannabinol (THC) are the two main components of cannabis that provide its therapeutic benefits. CBD has been extensively studied for its role in reducing seizures, among its many other uses. While the exact mechanisms by which CBD works to relieve seizures have not yet been fully determined, it is evident that CBD effectively diminishes seizure activity and is now being used as an approved treatment for severe forms of non-responsive epilepsy. As essential components of several biological processes, microRNAs (miRNAs) are crucial for achieving optimal cellular functioning. During the past few years, there has been an increasing interest in elucidating the functional significance of microRNAs in epilepsy. Yet, there is still considerable ambiguity regarding the precise mechanisms by which miRNAs are involved in seizure disorders and also their direct interaction with CBD. Herein, we seek to present an overview of the varying mechanisms by which CBD offers therapeutic benefits concerning seizures. Accordingly, we highlighted the shared molecular targets between microRNAs and CBD in alleviating seizure symptoms to shed light on the ways in which new therapeutic and diagnostic modalities could be shaped in the future.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1011-1022"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of chemical and blood transition components of Xipayi Maizibizi Oral Liquid and its excitatory purine regulation-mechanism study in treating overactive bladder. 喜帕依麦子滋口服液化学成分、血运成分分析及治疗膀胱过动症的兴奋性嘌呤调节机制研究。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-08-04 DOI: 10.1093/jpp/rgaf011

Menglu Wang, Yang Yang, Jiamei Xie, Yuhang Du, Yige Zhao, Yongcheng An, Ziyi Shan, Changhao He, Wanxin Fu, Yan Huang, Huilin Zhang, Baosheng Zhao

{"title":"Analysis of chemical and blood transition components of Xipayi Maizibizi Oral Liquid and its excitatory purine regulation-mechanism study in treating overactive bladder.","authors":"Menglu Wang, Yang Yang, Jiamei Xie, Yuhang Du, Yige Zhao, Yongcheng An, Ziyi Shan, Changhao He, Wanxin Fu, Yan Huang, Huilin Zhang, Baosheng Zhao","doi":"10.1093/jpp/rgaf011","DOIUrl":"10.1093/jpp/rgaf011","url":null,"abstract":"Objective: To explore the chemical components and blood transition components of Xipayi Maizibizi Oral Liquid (XP) as well as the efficacy and mechanism of XP in the treatment of overactive bladder (OAB).Methods: The chemical components and blood transition components were analysed. Rats were undergone bladder outlet obstruction surgery and divided into five groups. The study observed the general conditions, urodynamic argument and the bladder histopathological changes. The expression levels of ATP and P2X3 were evaluated.Results: One hundred and eighty-six chemical components were identified in XP, including 26 phenolic acids, 37 flavonoids, 7 quinones, 11 lignans and coumarins, 8 tannins, 9 alkaloids, 37 terpenoids, 32 steroids, and 19 other components. One hundred and twenty-eight components of XP were found in the blood, including 23 phenolic acids, 22 flavonoids, 3 quinones, 8 lignans and coumarins, 7 tannins, 7 alkaloids, 21 terpenoids, 20 steroids, and 17 other components. XP demonstrated effective treatment of OAB and downregulated the expression of ATP and P2X3.Conclusion: The characterization of the chemical composition and blood transition components of XP provided a foundation for further pharmacodynamic material basis and quality control of XP. XP could enhance the pathological status of rats with OAB by regulating ATP/P2X3.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1023-1041"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction of: Potential therapeutic efficacy of pachymic acid in chronic kidney disease induced in rats: role of Wnt/β-catenin/renin-angiotensin axis. 厚青酸对大鼠慢性肾病的潜在治疗作用：Wnt/β-连环蛋白/肾素-血管紧张素轴的作用。

IF 3.2 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-28 DOI: 10.1093/jpp/rgaf061

引用次数: 0

Mechanisms and potential roles of active ingredients of traditional Chinese medicine in the treatment of chronic obstructive pulmonary disease. 中药有效成分治疗慢性阻塞性肺疾病的机制及潜在作用。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-01 DOI: 10.1093/jpp/rgaf018

Xilin Wu, Yonghu Chen, Hanyu Zhang, Jiamin Wang, Chenchen Tian, Zhe Jiang, Xuezheng Li

{"title":"Mechanisms and potential roles of active ingredients of traditional Chinese medicine in the treatment of chronic obstructive pulmonary disease.","authors":"Xilin Wu, Yonghu Chen, Hanyu Zhang, Jiamin Wang, Chenchen Tian, Zhe Jiang, Xuezheng Li","doi":"10.1093/jpp/rgaf018","DOIUrl":"10.1093/jpp/rgaf018","url":null,"abstract":"Objectives: Chronic obstructive pulmonary disease (COPD) is a respiratory condition with high rates of morbidity and mortality. Recent studies have shown that the increasing research on Traditional Chinese Medicine (TCM) also plays an important role in COPD. The purpose of this review is to categorize TCM and its active ingredients and to summarize their pharmacological effects.Methods: Articles published up to December 2024 were searched through PubMed, X-MOL, and the China National Knowledge Infrastructure. The keywords included TCM and its combination with COPD, pharmacologic activity, anti-inflammatory effects, pharmacology, as well as in vivo and in vitro studies.Key findings: Thus far, we have summarized the progress of research on the mechanisms of action of TCM and its active ingredients, such as flavonoids, terpenoids, and phenols, in the treatment of COPD. These mechanisms encompass the reduction of inflammatory responses and lung injury, regulation of the oxidation-antioxidation balance, and modulation of cellular apoptosis and aging, among other effects.Conclusion: TCM and its active ingredients demonstrate strong anti-COPD properties. This provides a reference for accelerating the development of herbal components for the treatment of COPD and for exploring new potential multi-target therapeutic mechanisms. This will mitigate the geographical limitations of using TCM and enhance its application in future management strategies.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"866-883"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shaoyao Gancao decoction, an Ancient Classical Prescription: a review on its chemical composition, pharmacology, pharmacokinetics, clinical applications, and toxicology. 古代经典处方芍药甘草汤的化学成分、药理学、药代动力学、临床应用及毒理学研究综述。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-01 DOI: 10.1093/jpp/rgaf017

Zhang Facheng, Qiu Rongli, Zhang Li, Wu Baoxiang, Yu Sheng, Shan Mingqiu

{"title":"Shaoyao Gancao decoction, an Ancient Classical Prescription: a review on its chemical composition, pharmacology, pharmacokinetics, clinical applications, and toxicology.","authors":"Zhang Facheng, Qiu Rongli, Zhang Li, Wu Baoxiang, Yu Sheng, Shan Mingqiu","doi":"10.1093/jpp/rgaf017","DOIUrl":"10.1093/jpp/rgaf017","url":null,"abstract":"Objectives: Shaoyao Gancao decoction (SGD) is a famous Ancient Classical Prescription (ACP) from \"Treatise on Febrile Diseases.\" It has been clinically used for spasm- and pain-related disorders induced by insufficiency of Qi and blood and malnutrition of tendons and vessels for thousands of years. To expand comprehensive understanding and to highlight the importance of more effective utilization, this study aimed to provide a comprehensive review of SGD covering multiple research fields.Methods: Some databases, including PubMed, Web of Science, Google Scholar, and China National Knowledge Infrastructure, were used to collect the related information with \"Shaoyao Gancao decoction\" and similar ones as the keywords.Key findings: Phytochemical researches revealed that flavonoids and monoterpenoids were the predominant components in SGD. It was documented that SGD had demonstrated a variety of effects, such as analgesic and anti-inflammatory activity, neuroprotection, antispasmodic activity, gastrointestinal protection, hepatoprotection, anti-asthma activity, and effects on gynecological diseases. As for its toxicology, pseudoaldosteronism occasionally occurred and 18β-glycyrrhetyl-3-O-sulfate was believed to be a causative agent.Conclusions: As a whole, many valuable achievements have been made, exhibiting great attraction and potential of SGD as a famous ACP. This review is also expected to facilitate SGD application and research in the future.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"845-865"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Griffonia simplicifolia seeds extract rich in 5-hydroxy-L-tryptophan reduces infection and inflammation in a mouse model of vulvovaginal candidiasis. 富5-羟基- l -色氨酸的栀子提取物可降低外阴阴道念珠菌病小鼠模型的感染和炎症。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-01 DOI: 10.1093/jpp/rgaf024

Matteo Puccetti, Alessandro Di Michele, Cinzia Pagano, Elisa Pecorari, Alice Coletti, Leonardo Tensi, Marilena Pariano, Rita Pecorari, Maurizio Ricci, Luana Perioli

{"title":"Griffonia simplicifolia seeds extract rich in 5-hydroxy-L-tryptophan reduces infection and inflammation in a mouse model of vulvovaginal candidiasis.","authors":"Matteo Puccetti, Alessandro Di Michele, Cinzia Pagano, Elisa Pecorari, Alice Coletti, Leonardo Tensi, Marilena Pariano, Rita Pecorari, Maurizio Ricci, Luana Perioli","doi":"10.1093/jpp/rgaf024","DOIUrl":"10.1093/jpp/rgaf024","url":null,"abstract":"Background: Griffonia simplicifolia seeds extracts, generally prepared by maceration, are most commonly used in food supplements intended for mental well-being promotion due to the high content of 5-hydroxy-L-tryptophan (5-HTP), direct serotonin precursor. As 5-HTP is involved in many different biochemical pathways, other applications of G. simplicifolia seed extracts were investigated. Considering the well-known serotonin immunomodulatory activity, the object of this study was to optimize 5-HTP yield by a new extraction procedure, exploit its immunomodulatory activity in a preclinical model of vulvovaginal candidiasis (VVC) for its responsiveness to immunomodulation, and formulate the extract in a suitable vaginal formulation.Methods: High-power ultrasonic technique (HPU) was used for the first time to obtain an extract from Griffonia seeds (GSE-HPU). The amount of 5-HTP was measured by UHPLC-MS/MS. The extract was assessed in C57BL/6 mice model intravaginally infected with C. albicans.Results: The results showed that the new extraction procedure greatly increased 5-HTP yield (82.8% w/w of the extract weight); formulated in \"tablets for vaginal solution\" properly characterized, the GSE-HPU showed a remarkable protective activity in VVC as reduces inflammation, tissue pathology, and fungal growth.Conclusions: The protective efficacy of GSE-HPU in VVC suggests its potential use in vaginal infections and associated inflammatory pathology.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"933-943"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of Calophyllum brasiliense leaves extract and isolated compound amentoflavone: implications in the resolution of inflammation. 嫩叶提取物和分离的复方阿门托黄酮对炎症反应的影响。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-01 DOI: 10.1093/jpp/rgaf009

Larissa Benvenutti, Guilherme Moreschi Gerhardt, Ruth Meri Lucinda, Otto Maurício Santos Gerlach, Valdir Cechinel-Filho, Luiz Carlos Klein-Júnior, Nara Lins Meira Quintão, José Roberto Santin

{"title":"The effects of Calophyllum brasiliense leaves extract and isolated compound amentoflavone: implications in the resolution of inflammation.","authors":"Larissa Benvenutti, Guilherme Moreschi Gerhardt, Ruth Meri Lucinda, Otto Maurício Santos Gerlach, Valdir Cechinel-Filho, Luiz Carlos Klein-Júnior, Nara Lins Meira Quintão, José Roberto Santin","doi":"10.1093/jpp/rgaf009","DOIUrl":"10.1093/jpp/rgaf009","url":null,"abstract":"Calophyllum brasiliense is employed in folk medicine as an analgesic and to treat inflammation. This study aimed to evaluate the anti-inflammatory effect of C. brasiliense leaves' methanol extract, fractions, and the isolated compound amentoflavone. In vitro fMLP-induced neutrophil chemotaxis and LPS-induced inflammatory mediator levels were assessed in neutrophils or macrophages treated or not with different concentrations of C. brasiliense extract, its fractions or amentoflavone. Their effects on inflammation resolution was assessed by evaluating the efferocytosis. The extract and its fractions (DFCB, AFCB, and MFCB) impaired neutrophil migration stimulated by the chemotactic mediator fMLP and its ability to produce and/or to release cytokines (TNF and IL-6) and NO. The increase of the apoptotic neutrophil efferocytosis was observed for cells treated with the ethyl acetate and methanol fractions, accompanied by the enhanced IL-10 levels in the supernatant and the decrease of TNF, as well. Amentoflavone, present in high concentration in ethyl acetate fraction, reduced the inflammatory mediators levels in LPS-stimulated macrophages, impaired the neutrophil chemotaxis, and enhanced the efferocytosis. The obtained data demonstrate that C. brasiliense extract presented anti-inflammatory effects by modulating neutrophil migration/activation, macrophage-dependent efferocytosis, and inflammatory mediator release, effects at least partly addressed to amentoflavone content.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"961-969"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the mechanism of pachymic acid intervention in myocardial ischemia based on network pharmacology and experimental validation. 基于网络药理学和实验验证探讨厚青酸干预心肌缺血的机制。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-01 DOI: 10.1093/jpp/rgae153

Nengpin Yin, Xuan Zhao, Jin Yang, Zongjun Liu

{"title":"Exploring the mechanism of pachymic acid intervention in myocardial ischemia based on network pharmacology and experimental validation.","authors":"Nengpin Yin, Xuan Zhao, Jin Yang, Zongjun Liu","doi":"10.1093/jpp/rgae153","DOIUrl":"10.1093/jpp/rgae153","url":null,"abstract":"Objectives: To deeply explore the mechanism of pachymic acid (PA) intervention in myocardial ischemia, providing new ideas and methods for the treatment of myocardial ischemia.Methods: Predict the targets of PA for improving myocardial ischemia, and conduct functional enrichment analysis using databases, such as Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Reactome. To verify these findings, PPI network topology analysis and molecular docking were used to screen key targets and main mechanisms of action and further validated through in vitro experiments on the H9C2 cell line.Key findings: PA can significantly alleviate myocardial damage caused by hypoxia/reoxygenation, effectively reversing the abnormalities of oxidative stress indicators such as LDH, MDA, SOD, and ROS. PA may exert its effects through 39 targets, particularly by regulating the downregulation of autophagy-related proteins LC3-II and Beclin-1 expression via MTOR, thereby inhibiting excessive autophagy in cardiomyocytes.Conclusions: PA protects myocardial cells during myocardial ischemia through various pathways, particularly by regulating mTOR to inhibit excessive autophagy.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"944-960"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can Quercetin protect against the pre-disposing factors for Alzheimer's disease via inhibiting NLRP3 inflammasome pathway? 槲皮素能否通过抑制NLRP3炎性体通路来预防阿尔茨海默病的易感因素？

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-01 DOI: 10.1093/jpp/rgaf020

Azza S Awad, Bassant M El-Mokadem, Miar M Sherif, Abeer Bishr

{"title":"Can Quercetin protect against the pre-disposing factors for Alzheimer's disease via inhibiting NLRP3 inflammasome pathway?","authors":"Azza S Awad, Bassant M El-Mokadem, Miar M Sherif, Abeer Bishr","doi":"10.1093/jpp/rgaf020","DOIUrl":"10.1093/jpp/rgaf020","url":null,"abstract":"Objectives: The brain and its cognitive functions are most liable to stress, where it is known to promote the pathological manifestation of Alzheimer's disease (AD). This study aimed to investigate the effect of a 2-week-period of restraint stress (RS), as well as the protective effect of Quercetin in a dose-dependent manner against the early start of AD via studying the NLRP3 inflammasome pathway.Methods: The rats were divided into four groups: control (Con), induction (Ind), where 6 h/day for 2 weeks of RS was induced, low and high doses of Q (Q1+Ind and Q2+Ind, respectively), which were administered before the induction of RS for the same period. Behavioral tests were performed to assess the cognitive functions.Key findings: The higher dose of Q has shown more inhibition of the NLRP3 inflammasome pathway, oxidative stress, as well as the phosphorylated-tau and amyloid-β (Aβ) protein, which were significantly fired up by the 2 weeks of RS. These results were backed with the improved cellular structure in the histopathological examination and enhanced cognitive functions, where the two doses of Q have shown their protective effect.Conclusions: This proves that Q shielded the brain against the initial pathogenesis of AD, induced by 2 weeks of RS.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"911-921"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of natural phenolics-rich extracts and formulations based on Putoria calabrica leaf for wound healing applications. 开发和表征天然酚类物质丰富的提取物和配方的基础上，芦荟叶的伤口愈合应用。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-07-01 DOI: 10.1093/jpp/rgaf010

Nasreddine Mekhoukh, Nadia Chougui, Ana A Vilas-Boas, Manuela Pintado, Hamdi Bendif, Mirella Zancato, Youba Bellik, Nassim Sid, Gregorio Peron

{"title":"Development and characterization of natural phenolics-rich extracts and formulations based on Putoria calabrica leaf for wound healing applications.","authors":"Nasreddine Mekhoukh, Nadia Chougui, Ana A Vilas-Boas, Manuela Pintado, Hamdi Bendif, Mirella Zancato, Youba Bellik, Nassim Sid, Gregorio Peron","doi":"10.1093/jpp/rgaf010","DOIUrl":"10.1093/jpp/rgaf010","url":null,"abstract":"Background and objectives: This study is the first to comprehensively investigate the phenolic profile, therapeutic potential, and acute toxicity of Putoria calabrica, a Mediterranean medicinal plant. It aims to evaluate its potential for innovative wound healing formulations by analyzing the phenolic composition of five extracts, assessing antifungal activity, and evaluating toxicity, hemoglobin oxidative status, and wound healing efficacy in animal models.Methods: The phenolic content of the extracts was analyzed using HPLC-DAD. Antifungal activity was assessed on solid PDA media, while biochemical parameters were determined spectrophotometrically.Key findings: Ten phenolics were identified, with vitexin (20.84 mg/g), rutin (17.66 mg/g), and chlorogenic acid (14.15 mg/g) as the predominant. Methanol extract showed the highest antifungal activity against Fusarium oxysporum and Penicillium chrysogenum with rates of 57.61% and 59.62% inhibition respectively, and a Minimum Inhibitory Concentration of 8 mg/ml, comparable to ethanol extract. The latter also inhibited hemoglobin degradation and methemoglobin formation at 2.5-5.0 mg/ml. In mice, ethanol extract ointments (5% and 10%) showed no toxicity, with a 96.43% wound contraction after 18 days of applying the 10% formulation.Conclusions: The current findings suggest that P. calabrica leaf extracts may offer a promising natural remedy with wound healing, antioxidant, and antifungal properties, deserving further investigation for therapeutic applications.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"970-982"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0