Journal of proteomics最新文献

{"title":"Prospective serial proteomic analysis uncovers mechanistic pathways of chemotherapy resistance in advanced non-small cell lung cancer","authors":"Wei-Ke Kuo ,&nbsp;Hsin-Yu Chu ,&nbsp;Yen-Kun Ko ,&nbsp;Pang-Hung Hsu","doi":"10.1016/j.jprot.2026.105620","DOIUrl":"10.1016/j.jprot.2026.105620","url":null,"abstract":"<div><div>Predicting chemotherapy response in advanced non-small cell lung cancer (NSCLC) remains a clinical challenge, as baseline profiles often fail to capture dynamic molecular adaptations under treatment. This prospective study employed serial plasma proteomics to identify mechanistic pathways associated with chemotherapy resistance in 44 patients with stage IV NSCLC receiving platinum-based doublet chemotherapy. By analyzing blood samples collected immediately before the first and second cycles using liquid chromatography-tandem mass spectrometry, we demonstrated that a ratio-based proteomic model (early-treatment/pre-treatment) yielded superior separation between controlled and uncontrolled disease (UCD) compared to baseline-only assessment. Among 159 quantified proteins, 13 showed significant differential abundance, with UCD patients exhibiting marked upregulation of tetranectin, coagulation factor XIII A chain, and complement factor H-related protein 2. Ingenuity Pathway Analysis revealed that therapeutic resistance was characterized by three dominant axes: the activation of complement-coagulation-acute-phase signaling, the induction of lipid-nuclear receptor activity (LXR/RXR and DHCR24 signaling), and the relative attenuation of immune-regulatory pathways such as IL-12 signaling. These findings highlight the potential of serial proteomic profiling to uncover treatment-induced molecular adaptations, providing insights for therapeutic monitoring and hypothesis generation in precision oncology.</div></div><div><h3>Significance</h3><div>This study demonstrates the added value of prospective serial plasma proteomic profiling, compared with baseline-only approaches, for capturing early treatment-associated molecular adaptations in advanced non-small cell lung cancer (NSCLC) receiving chemotherapy. By quantifying proteomic changes between pre-treatment and early-treatment time points, we identified coordinated alterations involving the complement–coagulation–acute-phase axis and lipid–nuclear receptor signaling programs, including LXR/RXR and DHCR24, alongside relative attenuation of immune-regulatory pathways.</div><div>Rather than reflecting isolated protein effects, these findings highlight interconnected host–tumor response programs that emerge under therapeutic pressure and may contribute to early adaptive resistance. Importantly, this work moves beyond static baseline markers by emphasizing dynamic, pathway-level changes and provides a hypothesis-generating framework for longitudinal therapeutic monitoring. Candidate proteins such as tetranectin and coagulation factor XIII A chain are proposed as molecular features associated with treatment response, warranting further validation in larger, prospective cohorts before translational application.</div></div>","PeriodicalId":16891,"journal":{"name":"Journal of proteomics","volume":"326 ","pages":"Article 105620"},"PeriodicalIF":2.8,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146137258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics analysis of brain tissues of cerebral ischemic rat treated with Xingnaojing injection and its brain component muscone","authors":"Chunli Gao ,&nbsp;Han Hao ,&nbsp;Xufei Chen ,&nbsp;Niuniu Mao ,&nbsp;Xiaoyan Zhang ,&nbsp;Juanjuan Zhao ,&nbsp;Yanfeng Li ,&nbsp;Nan Deng ,&nbsp;Pu Jia ,&nbsp;Xiaohui Zheng ,&nbsp;Sha Liao ,&nbsp;Yangyang Bian","doi":"10.1016/j.jprot.2026.105610","DOIUrl":"10.1016/j.jprot.2026.105610","url":null,"abstract":"<div><div>Cerebral ischemia-reperfusion injury is categorized as “stroke” in traditional Chinese medicine. For thousands of years, traditional Chinese medicine has accumulated rich experience in the treatment of stroke and other diseases, and with remarkable curative effects. Currently, Xingnaojing injection and its component musk are commonly used in the treatment of acute stroke, and muscone is the main active ingredient of musk. In this study, a rat model of transient middle cerebral artery occlusion was established, and the neuroprotective effects of Xingnaojing and muscone on transient middle cerebral artery occlusion rats were validated by Zea-Longa neurological function score, behavioral test and 2,3,5-triphenyltetrazolium chloride staining. Quantitative proteomics analysis was then performed on the brain tissues from different groups to investigate the mechanisms by which Xingnaojing and muscone act on cerebral ischemia-reperfusion injury. Our data indicate that Xingnaojing and muscone significantly affect proteins related to oxidative phosphorylation in CIRI rats, highlighting mitochondrial energy metabolism as a potentially important pathway contributing to their neuroprotective effects. Furthermore, the limited proteolysis-coupled mass spectrometry, target-responsive accessibility profiling, and lysine reactivity profiling methods were used to identify the direct protein targets of muscone in rat brain tissue lysate. A total of 36 potential target proteins were commonly identified by all the three methods. Bioinformatics analysis suggested that muscone was more significantly enriched in glycolysis/gluconeogenesis related pathways and closely associated with oxidative phosphorylation. Finally, the glycolytic key enzyme phosphoglycerate kinase 1, one of the binding proteins with muscone, was selected and verified by drug affinity responsive target stability. The molecular docking and dynamics simulation analysis further confirmed the interaction of glycolytic key enzyme phosphoglycerate kinase 1 and muscone. This study provides evidences for the clinical application and mechanisms of Xingnaojing and muscone in treating cerebral ischemia-reperfusion injury, and identifies candidate protein targets of muscone.</div></div>","PeriodicalId":16891,"journal":{"name":"Journal of proteomics","volume":"326 ","pages":"Article 105610"},"PeriodicalIF":2.8,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146086267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic profile of the extracellular matrix following cerebral ischemia-reperfusion injury identified HCK as a target for ischemic stroke therapy","authors":"Xiang Li ,&nbsp;Zhiqiong Mao ,&nbsp;Peichan Chao ,&nbsp;Yajin Liao ,&nbsp;Yalan Li","doi":"10.1016/j.jprot.2026.105609","DOIUrl":"10.1016/j.jprot.2026.105609","url":null,"abstract":"<div><div>Ischemic stroke is a detrimental central nervous system (CNS) disorder with high morbidity and disability rates, caused by local cerebral ischemia. Extracellular matrix (ECM) is a complex network structure secreted by cells and located in the intercellular compartment, which is significant changed following ischemic stroke.</div><div>Here, we quantified the proteomic profile of the ECM of brain from young (2-month-old) and aged (18-month-old) mice underwent cerebral ischemia/reperfusion (I/R) at 24 h and 60 d post I/R. The proteomics results indicated that proteins associated with the tricarboxylic acid (TCA) cycle and neutrophil extracellular trap (NET) formation were significantly up-regulated in the brain ECM from mice underwent cerebral I/R during acute stage, while those associated with synaptic vesicle cycle were significantly down-regulated in all stage post cerebral I/R. Differently, the brain ECM from aged mice underwent I/R expressed higher levels of lysosomal proteins and lower levels of autophagy and synaptic vesicle cycle associated proteins than the brain ECM from young mice underwent I/R. Furtherly, the proteomics identified that Hematopoietic Cell Kinase (HCK) is a regulator for NET formation. Inhibition of HCK could down-regulate LPS-induced phosphorylation of ERK1/2 and IKKα/β, as well as blocking the LPS plus nigericin induced activation of NLRP3 inflammasome and NET-like trap formation <em>in vitro</em>. In addition, inhibition of HCK significantly ameliorated cerebral I/R-induced brain injury and NET formation <em>in vivo</em>, suggesting HCK is a therapeutic target for ischemic stroke treatment.</div></div><div><h3>Significance</h3><div>Our results systemically analyzed the protein profiles of ECM in the brain post- acute and chronic ischemic stroke, and identified upregulation of NET-associated proteins was a common feature of the cerebral ECM during the acute phase, while down-regulation of synaptic vesicle cycle associated proteins was a common character of the brain ECM in all stage. What's more, HCK was identified as a regulator for NET formation, inhibition of HCK could block the formation of NET by inhibiting the activation of ERK1/2, IKKα/β and NLRP3 inflammasome, suggesting HCK is a therapeutic target for ischemic stroke treatment.</div></div>","PeriodicalId":16891,"journal":{"name":"Journal of proteomics","volume":"326 ","pages":"Article 105609"},"PeriodicalIF":2.8,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-dimensional electrophoresis-based proteomics reveals soybean seed hypocotyl proteoforms","authors":"Jian-Zhong Tan ,&nbsp;Hiroyuki Kagawa ,&nbsp;Keiko Kizawa ,&nbsp;Hisashi Hirano","doi":"10.1016/j.jprot.2026.105600","DOIUrl":"10.1016/j.jprot.2026.105600","url":null,"abstract":"<div><div>Shotgun proteomics is widely used for comprehensive profiling of protein expression. However, this approach has inherent limitations in resolving “proteoforms”. Two-dimensional gel electrophoresis (2DE) has the potential to separate and visualize proteoforms effectively. To evaluate its utility, proteins extracted from soybean seed hypocotyls were analyzed by 2DE/LC-MS/MS. As a result, a total of 693 proteins were separated by 2DE, of which 302 were identified by LC-MS/MS. The dynamic range of protein abundance was approximately 10² to 10⁴. This analysis revealed that the hypocotyls contain numerous proteoforms of proteins essential for seed physiology, including late embryogenesis abundant protein, glycinin, β-conglycinin, trypsin inhibitor, sucrose-binding protein, and glyceraldehyde-3-phosphate dehydrogenase. Furthermore, 2DE revealed that the expression of proteoforms of the major seed storage proteins, glycinin A2 and A3 subunits, and β-conglycinin β subunit in hypocotyls differed from that in cotyledons, suggesting distinct functional roles beyond nutrient storage during germination. Overall, the results demonstrate that 2DE is a valuable complementary technique to shotgun proteomics, providing proteoform-specific information that cannot be resolved by shotgun analysis alone.</div></div><div><h3>Significance</h3><div>Shotgun proteomics is widely used for comprehensive profiling of protein expression. However, this approach has inherent limitations when analyzing “proteoforms”. In the present study, we analyzed soybean seed hypocotyls using two-dimensional gel electrophoresis (2DE)-LC-MS/MS, demonstrating that 2DE is useful for effective proteoform analysis. 2DE provides proteoform-specific information that cannot be obtained from shotgun proteomics alone, making it a useful complementary method.</div></div>","PeriodicalId":16891,"journal":{"name":"Journal of proteomics","volume":"326 ","pages":"Article 105600"},"PeriodicalIF":2.8,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The in vitro effect of omentin-1 on the global proteome of granulosa cells from normal weight Large White and fat Meishan pigs","authors":"Karolina Pich ,&nbsp;Natalia Respekta-Długosz ,&nbsp;Edyta Rytelewska ,&nbsp;Bianka Świderska ,&nbsp;Agata Malinowska ,&nbsp;Nina Smolińska ,&nbsp;Joëlle Dupont ,&nbsp;Agnieszka Rak","doi":"10.1016/j.jprot.2026.105606","DOIUrl":"10.1016/j.jprot.2026.105606","url":null,"abstract":"<div><div>Ovarian granulosa cells (Gc) play a vital role in follicle maturation and successful ovulation. Omentin-1 (ITLN1) is an adipokine involved in energy metabolism and insulin resistance; its expression has been demonstrated in the ovary and varies depending on the degree of pig fatness. However, its effect on the global proteome of Gc has not been previously investigated. It was hypothesized that ITLN1 affects the abundance of proteins involved in key processes occurring in Gc in pigs. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of Gc identified 208 significantly differentially abundant proteins (DAPs) in the Large White pigs, with 99 proteins upregulated and 109 downregulated. In fatter Meishan pigs, 42 statistically significant DAPs were identified, including 25 upregulated and 17 downregulated proteins. The identified DAPs were associated with the estrogen signaling pathway, cell cycle and DNA replication, protein synthesis, transport and maturation, cytoskeleton dynamics, cell signaling, and hormonal regulation. Notably, the number and identity of DAPs differed markedly between the two breeds, suggesting that ITLN1-mediated effects are modulated by fatness and breed-specific metabolic status. To further illustrate the observed differences, selected proteins were also analyzed using Western blotting and ELISA, which were consistent with the LC-MS/MS findings. The results indicate that ITLN1 has a modulatory influence on the porcine Gc proteome, which is dependent on fat content. This highlights the important role of ITLN1 in regulating ovarian functions.</div></div><div><h3>Significance</h3><div>This study provides a comprehensive proteomic analysis of porcine granulosa cells (Gc) after treatment with omentin-1 (ITLN1) in pigs with different fat content (Large White &lt; Meishan). The identified differentially abundant proteins (DAPs) are intricately linked to critical biological pathways, including estrogen signaling, cell cycle regulation, DNA replication, protein synthesis and transport, cytoskeleton organization, and hormonal regulation. These findings enhance our understanding of the molecular mechanisms underpinning ovarian follicle development and breed-related reproductive traits in pigs. The insights gained could inform future strategies to improve fertility and reproductive efficiency in swine production, as well as provide a valuable resource for comparative studies on ovarian biology across species.</div></div>","PeriodicalId":16891,"journal":{"name":"Journal of proteomics","volume":"326 ","pages":"Article 105606"},"PeriodicalIF":2.8,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early regulatory networks driving somatic embryogenesis in Saccharum spp. L. revealed by time-resolved proteomics","authors":"Roberta Pena da Paschoa ,&nbsp;Lucas Rodrigues Xavier ,&nbsp;Caio Cezar Guedes Corrêa ,&nbsp;Karina da Silva Vieira ,&nbsp;Daniel Dastan Rezabala Pacheco ,&nbsp;Lucas do Espirito Santo Gomes ,&nbsp;Carlos Eduardo Assis da Silva ,&nbsp;Laura Eliza de Oliveira Alves ,&nbsp;Vitor Batista Pinto ,&nbsp;Claudete Santa-Catarina ,&nbsp;Vanildo Silveira","doi":"10.1016/j.jprot.2026.105602","DOIUrl":"10.1016/j.jprot.2026.105602","url":null,"abstract":"<div><div>The induction of somatic embryogenesis is controlled by various genes and proteins involved in hormonal pathways and stress responses, which act as key regulators of in vitro cellular reprogramming. In this study, we employed a temporal proteomic approach to investigate the underlying molecular mechanisms governing sugarcane (<em>Saccharum</em> spp.) embryogenic callus formation in response to 2,4-dichlorophenoxyacetic acid (2,4-D) during induction. Proteomic profiling revealed 996 differentially accumulated proteins (DAPs) across at least one pairwise comparison among time points (0, 7, 14 and 21 days) during callus induction. These DAPs were classified into different clusters on the basis of their accumulation profile. Proteins involved in embryogenesis, histone epigenetic regulation, hormone responses and protein post-translational modification accumulate during callus induction. The predicted interactions between the TOPLESS protein and auxin response proteins (SKP1, CUL1 and CAND1) are associated with increased accumulation of the histone deacetylase HDT2 protein, a regulator of chromatin condensation, during embryogenic callus initiation. Moreover, proteomic analysis revealed a temporal reduction in methylation cycle enzymes during callus induction, whereas global DNA methylation showed only a slight, non-significant increase, suggesting that additional regulatory layers are present. The identified protein dynamics provide valuable targets for refining somatic embryogenesis protocols and advancing their biotechnological applications in sugarcane.</div></div><div><h3>Significance</h3><div>Genetic engineering and plant cloning usually involve the induction of embryogenic competence using 2,4-dichlorophenoxyacetic acid (2,4-D). This study presents protein-protein interaction (PPI) networks regulated during the induction of sugarcane callus using 2,4-D, in addition to the morphological aspects of the explant during the process. Proteomic analysis of time series shows the regulation of protein kinases and transcriptional regulators TOPLESS, CUL1, SKP1, CAND1, and ARGONAUTE kinases, revealing mechanisms of activation of induction and multiplication of embryogenic callus. Furthermore, the possible interaction between GH3.8 and SnRK/SAPK kinases suggests a link between hormonal responses.</div></div>","PeriodicalId":16891,"journal":{"name":"Journal of proteomics","volume":"326 ","pages":"Article 105602"},"PeriodicalIF":2.8,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145986725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time-resolved proteomic and phosphoproteomic profiling of Angiotensin-(1–7) signaling in A549 cells","authors":"Marcella N. Melo-Braga ,&nbsp;Gabriela C. Magalhães ,&nbsp;Filipe A. Silva ,&nbsp;Frank Kjeldsen ,&nbsp;Martin R. Larsen ,&nbsp;Robson A.S. Santos ,&nbsp;Thiago Verano-Braga","doi":"10.1016/j.jprot.2026.105607","DOIUrl":"10.1016/j.jprot.2026.105607","url":null,"abstract":"<div><div>Angiotensin-(1–7) [Ang-(1–7)] is a heptapeptide of the renin-angiotensin system (RAS) with antitumoral effects reported in various tumoral cell lines, including the human lung adenocarcinoma A549 lineage. While previous studies have shown that Ang-(1–7) modulates MAPK and PI3K-AKT signaling, the precise molecular mechanisms involved remain incompletely understood. To investigate the signaling events of Ang-(1–7) in lung cancer-derived cells, we employed an integrated proteomic and phosphoproteomic approach in A549 cells. We analyzed early (minutes) and late (hours) molecular responses to Ang-(1–7) treatment. The treatment resulted in time-dependent modulation of multiple signaling pathways, including significant alterations in the MAPK, PI3K-AKT, and mTOR pathways at both the protein and phosphorylation levels. Notably, widespread early dephosphorylation events were observed, similar to the effects seen with other RAS peptides with antitumoral effects. Additionally, Ang-(1–7) promoted a long-lasting nuclear accumulation (up to 24 h) of the transcription factor FOXO1 indicating its activation. FOXO1 is known to regulate genes involved in apoptosis, cell cycle arrest, and oxidative stress, suggesting a role in mediating the peptide's antitumoral effects. The study provides new insights into the molecular basis of Ang-(1–7)’s antitumoral activity in A549 cells and reinforce its therapeutic potential in lung cancer. Raw data are available via ProteomeXchange with identifier <span><span>PXD066687</span><svg><path></path></svg></span>.</div></div><div><h3>Significance</h3><div>This study provides the first comprehensive, time-resolved proteomic and phosphoproteomic analysis of Angiotensin-(1–7) signaling in the lung cancer cell line A549. By capturing both early and late molecular events in A549 cells, we reveal that Ang-(1–7) modulates critical pathways involved in tumor progression, including MAPK, PI3K-AKT, and mTOR signaling. Importantly, we demonstrate the nuclear accumulation of FOXO1, a key transcription factor associated with tumor suppression, as part of the Ang-(1–7) response in A549 cells.</div></div>","PeriodicalId":16891,"journal":{"name":"Journal of proteomics","volume":"326 ","pages":"Article 105607"},"PeriodicalIF":2.8,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146025908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The untapped potential of metaproteomics in microbial systems ecology","authors":"Jingxuan Hu ,&nbsp;Yuan Zheng ,&nbsp;Leyuan Li","doi":"10.1016/j.jprot.2026.105604","DOIUrl":"10.1016/j.jprot.2026.105604","url":null,"abstract":"<div><div>As a direct readout of protein presence and abundance in complex microbiomes, metaproteomics has become central to uncovering functionality across diverse microbial ecosystems. Recent collaborative efforts within the research community have driven methodological advances, computational innovations, and diverse applications. With the advancing maturity of metaproteomics techniques, we highlight the Proteome-level Microbial Systems Ecology (ProMiSE) framework as a complementary framework for the next phase, providing a conceptual lens to interpret metaproteomics data with a bottom-up perspective that extends beyond functional profile and taxonomic composition to the quantitative assessment of system properties, processes, and dynamics. Building on existing metrics such as β-diversity and functional redundancy, future work can further develop quantitative approaches for resilience, exergy, and functional energy landscapes, thereby enabling a deeper systems-level understanding of microbiome dynamics and opening new avenues for the precise functional regulation of microbiomes.</div></div><div><h3>Significance</h3><div>This Perspective reviews the development of metaproteomics—particularly in the Journal of Proteomics—and envisions it as a transformative tool in microbial systems ecology, introducing the ProMiSE framework to integrate protein-resolved data with ecological theory and properties such as redundancy, resilience, and functional energy landscapes for the future of precise microbiome modulation.</div></div>","PeriodicalId":16891,"journal":{"name":"Journal of proteomics","volume":"326 ","pages":"Article 105604"},"PeriodicalIF":2.8,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146041021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary proteomics and metabolic responses to resistance training with and without blood flow restriction in young adults","authors":"Gustavo Zanete Alencar ,&nbsp;Dalton Muller Pessôa Filho ,&nbsp;Karina Oliveira Santos ,&nbsp;Anderson Geremias Macedo ,&nbsp;Henrique Basso Vitti ,&nbsp;Guilherme Neves Gasparino ,&nbsp;Larissa Tercília Grizzo Thomassian ,&nbsp;Murilo Henrique Faria ,&nbsp;Ana Carolina Magalhães","doi":"10.1016/j.jprot.2025.105587","DOIUrl":"10.1016/j.jprot.2025.105587","url":null,"abstract":"<div><div>Blood flow restriction (BFR) has been applied as alternative strategy to reproduce the effects of conventional high-load intensity training (HI) while training with low-loads (LI). Therefore, the aim of this research was to evaluate the salivary proteomic and metabolic responses to different resistance training in young adults.Ten participants were selected and underwent to training with LI (30 %1RM – one repetition maximum) plus BFR vs. HI (70 %1RM) without BFR, at 48 h-interval. Stimulated saliva was collected before and immediately after the sessions, while breath-by-breath oxygen uptake (VO₂) was measured during and after each session. Arterial blood samples for lactate concentration measurement (in EqO_2[La]) were taken at 1^st minute of resting between each exercise. For HI, there was an increase in two actin cytoplasmic isoforms and two immunoglobulin isoforms and a decrease of six hemoglobin isoforms. For LI-BFR, there was an increase in two hemoglobin isoforms, and the same immunoglobulin isoforms (<em>t</em>-test, <em>p</em> &lt; 0.05). No differences were significant between HI and LI + BRF training regarding the total energy demand (in mlO₂), absolute oxygen values (mlO₂) for oxidative response (VO_2ON), glycolytic (EqO_2[La]), and oxygen debt (VO_2OFF) (<em>p</em> &gt; 0.05). Both HI and LI + BFR protocols modulated immune system activity and exhibited divergent hemoglobin patterns.</div></div><div><h3>Significance</h3><div>The current study, which identified protein from saliva samples, a non-invasive method, and analyzed physiological markers, enabled the comparison of different resistance exercise protocols. Although no significant differences were observed in the metabolic responses to each protocol, which highlights the potential of LI + BRF to reproduce a high-intensity training stimulus, the changes in salivary protein profiles indicate specific functional adaptations that may become evident over time.</div></div>","PeriodicalId":16891,"journal":{"name":"Journal of proteomics","volume":"325 ","pages":"Article 105587"},"PeriodicalIF":2.8,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145843898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing systemic effects of Bothrops jararaca venom in the lungs in a mouse model by label-free proteomics using DDA and DIA","authors":"Ana Luiza T. Silva ,&nbsp;Bianca C.S.C. de Barros ,&nbsp;Joanderson P.C. da Silva ,&nbsp;Isabel S. Carvalho ,&nbsp;Marcelo L. Santoro ,&nbsp;Alison F.A. Chaves ,&nbsp;Solange M.T. Serrano","doi":"10.1016/j.jprot.2025.105588","DOIUrl":"10.1016/j.jprot.2025.105588","url":null,"abstract":"<div><div>Snakebite envenomation is a critical yet underexplored public health issue, particularly in tropical and subtropical regions. <em>Bothrops jararaca</em> venom induces severe local and systemic effects, including pulmonary injury, however, the molecular mechanisms underlying lung tissue damage remain poorly understood. This study employed label- free quantitative proteomics to map protein alterations in the lung tissue in a mouse model of envenomation. Using Data-Dependent Acquisition (DDA) and Data-Independent Acquisition (DIA) approaches combined with different sample preparation methods, we provide a comprehensive proteomic profile of venom-induced pulmonary damage. Our findings reveal significant changes in proteins involved in inflammatory responses, extracellular matrix remodeling, oxidative stress, and blood coagulation. Comparative analyses highlight the superior performance of DIA over DDA, with DIA offering deeper proteome coverage, enhanced detection of low-abundance proteins, and improved resolution of venom-induced alterations. This benchmark study underscores the potential of DIA as a robust tool for elucidating complex, systemic, mammalian molecular responses to animal toxins. By bridging the gap between proteomic methodologies and pathophysiological insights, our findings contribute to a deeper understanding of viperid snake venom-induced lung injury and give insights for improved clinical management strategies.</div></div><div><h3>Significance</h3><div><em>Bothrops</em> snakebites remain a major neglected health problem, causing severe local and systemic complications. While the impact of viperid venoms on muscle and kidney tissues is well documented, the effects on the lungs — an organ critically involved in systemic envenomation outcomes — remain poorly understood. Our study provides the first comprehensive proteomic characterization of lung responses to <em>B. jararaca</em> venom in a murine model, revealing alterations in pathways related to inflammation, extracellular matrix remodeling, oxidative stress, and coagulation. These molecular insights fill an important knowledge gap by showing that proteomic disturbances occur even in the absence of overt lung pathology, highlighting the lungs as a key systemic target of envenomation. Moreover, by demonstrating that antivenom administration mitigates many of these changes, our findings underscore both the therapeutic efficacy of antivenom and the need to better understand its broader systemic footprint. This work advances both toxinology and proteomics by linking molecular-level disturbances to clinically relevant systemic outcomes.</div></div>","PeriodicalId":16891,"journal":{"name":"Journal of proteomics","volume":"325 ","pages":"Article 105588"},"PeriodicalIF":2.8,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145850154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}