Journal of Poultry Science最新文献_第3页

Ovary Transcriptome Profiling in Broody and Egg-laying Chahua Chickens. 育雏鸡和产蛋茶花鸡的卵巢转录组分析。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2024-06-08 eCollection Date: 2024-01-01 DOI: 10.2141/jpsa.2024018

Yanli Du, Huang Wei, Meiquan Li, Xiao Wang, Jinshan Ran, Jing Wang, Cuilian Dong, Na Zhu

{"title":"Ovary Transcriptome Profiling in Broody and Egg-laying Chahua Chickens.","authors":"Yanli Du, Huang Wei, Meiquan Li, Xiao Wang, Jinshan Ran, Jing Wang, Cuilian Dong, Na Zhu","doi":"10.2141/jpsa.2024018","DOIUrl":"10.2141/jpsa.2024018","url":null,"abstract":"Broodiness in egg-laying hens (EHs) leads to ovarian atrophy, resulting in reduced egg-laying performance. However, the ovarian regulatory mechanisms in broody hens (BCs) remain elusive. Therefore, ovaries were removed from 300-day-old BCs and EHs for RNA sequencing. Ovarian morphology and histological characteristics of the BC and EH groups were compared and analyzed. The EH group had significantly more hierarchical follicles (HFs) and small yellow follicles (SYFs) than that of the BC group. Although several secondary follicles (SFs) and primary follicles were observed in the ovaries of the EH group, only a few SFs were observed in the ovaries of the BC group. Subsequently, RNA-sequencing analysis was conducted to determine the ovarian expression profiles of the two groups. Transcriptome sequencing identified 259 differentially expressed genes (DEGs) between the BC and EH groups. Of the 259 DEGs, 136 were upregulated and 123 were downregulated. The DEGs were mapped to 22 gene ontology terms and 4 Kyoto Encyclopedia of Genes and Genomes pathways for ovarian tissue. The analysis showed that matrix metalloproteinases 11/13 (MMP11/MMP13) were enriched in the extracellular matrix. The extracellular matrix mediated by MMP13 is affected by follicle-stimulating hormone, prolactin, and estrogen, which are critical signaling pathways that may affect ovarian follicle development to regulate the large yellow follicle reserve process and the ovulation cycle of broody Chahua chickens. These findings indicate that understanding differences in gene expression between the ovarian tissues of BCs and EHs could serve as a valuable reference point for enhancing egg-laying performance in Chahua chickens.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"61 ","pages":"2024018"},"PeriodicalIF":1.5,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embryonic Cadaverine Signaling: Implications for Plasma Free Amino Acid and Skeletal Muscle Energy Metabolism in Newly Hatched Chicks. 胚胎胎盘素信号传导：新孵化雏鸡血浆游离氨基酸和骨骼肌能量代谢的影响。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2024-06-06 eCollection Date: 2024-01-01 DOI: 10.2141/jpsa.2024017

Jun-Ichi Shiraishi, Naoko Shimakura, Kazuki Kimura, Ai-Saiga Egusa, Yoshiyuki Ohta

{"title":"Embryonic Cadaverine Signaling: Implications for Plasma Free Amino Acid and Skeletal Muscle Energy Metabolism in Newly Hatched Chicks.","authors":"Jun-Ichi Shiraishi, Naoko Shimakura, Kazuki Kimura, Ai-Saiga Egusa, Yoshiyuki Ohta","doi":"10.2141/jpsa.2024017","DOIUrl":"10.2141/jpsa.2024017","url":null,"abstract":"Cadaverine is a bioactive substance derived from lysine degradation by lysine decarboxylase and has gained attention for its physiological effects. Studies in rodents have revealed its role as a cell growth regulator, particularly intestinal bacterial-produced cadaverine. However, the nutritional and physiological roles of cadaverine during the embryonic period remain unclear, especially considering the immature state of the gut microbiota and digestive functions during this stage. This study explored the potential functions of cadaverine as a nutritional and metabolic signal during chicken embryonic development. Experiments were conducted using an in ovo administration method to evaluate the effects of nutritional bioactive substances on developing chicken embryos. Although there were no observable changes in body or organ weights of newly hatched chicks following in ovo cadaverine administration to day 18 chick embryos, plasma tryptophan, Nτ-methylhistidine, and Nπ-methylhistidine concentrations decreased and the gene expression of insulin/insulin-like growth factor 1 signaling in skeletal muscle was upregulated. These findings imply that cadaverine influences tryptophan metabolism and skeletal muscle catabolism during the embryonic period, suggesting its role as a bioactive factor contributing to energy metabolism signaling in skeletal muscle.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"61 ","pages":"2024017"},"PeriodicalIF":1.5,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11150007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141283912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survival Time of Campylobacter jejuni in Broiler Crops. 肉鸡养殖中空肠弯曲菌的存活时间

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2024-05-31 eCollection Date: 2024-01-01 DOI: 10.2141/jpsa.2024016

Mari Nishii, Masaharu Yasutomi

{"title":"Survival Time of Campylobacter jejuni in Broiler Crops.","authors":"Mari Nishii, Masaharu Yasutomi","doi":"10.2141/jpsa.2024016","DOIUrl":"10.2141/jpsa.2024016","url":null,"abstract":"Lactobacillus spp. inhibit the growth of Campylobacter spp. in vitro. However, in chicken crops, in which Lactobacillus spp. predominate, such inhibition of Campylobacter has not been confirmed. In our previous study, feeding paddy rice to broiler chicks increased the residence time of the food, which might enhance the bactericidal activity of the crop. Here, the bactericidal activity against the remaining Campylobacter spp. in broiler crops was evaluated. A suspension prepared by mixing Campylobacter jejuni and titanium dioxide (TiO2) was inoculated into the pharynx of 26-day-old broiler chicks fed a paddy rice-based diet. The crop contents were sampled at 20-min intervals. The TiO2 residual ratio in the crop gradually decreased with time after inoculation, with 57% of the inoculated TiO2 remaining in the crop 60 min after inoculation. The survival fraction of C. jejuni in the crops was 11% at 40 min, only 1% at 60 min, and was undetectable at 80 min. Most of the inoculated C. jejuni died in the crop before entering the next segment. These data indicated that bacterial death occurred between 30 min and 40 min after inoculation. The average survival time of C. jejuni in the crop was calculated to be 37.1 min. Thus, C. jejuni remaining in a chicken crop for more than 40 min died.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"61 ","pages":"2024016"},"PeriodicalIF":1.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11136615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and Mutation of SLC45A2 Affects Iris Color in Quail. SLC45A2 的表达和突变影响鹌鹑虹膜的颜色

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2024-05-30 eCollection Date: 2024-01-01 DOI: 10.2141/jpsa.2024015

Linke Huo, Xiaohui Zhang, Youzhi Pang, Yanxia Qi, Shiwei Ren, Fanghu Wu, Yuanyuan Shang, Jinquan Xi

{"title":"Expression and Mutation of SLC45A2 Affects Iris Color in Quail.","authors":"Linke Huo, Xiaohui Zhang, Youzhi Pang, Yanxia Qi, Shiwei Ren, Fanghu Wu, Yuanyuan Shang, Jinquan Xi","doi":"10.2141/jpsa.2024015","DOIUrl":"10.2141/jpsa.2024015","url":null,"abstract":"Iris color is a prominent phenotypic feature of quail. To understand the mechanism of melanin deposition related to quail iris color, iris tissues were selected from Beijing white and Chinese yellow quail for transcriptome analysis. Differentially expressed genes (DEGs) associated with pigmentation were identified using RNA sequencing and validated by quantitative real-time polymerase chain reaction (RT-qPCR). The identified single nucleotide polymorphisms were studied using bioinformatics and iris color correlation analyses. A total of 485 DEGs were obtained, with 223 upregulated and 262 downregulated. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed. Thirty-two genes were annotated using the GO database. Three important pigment synthesis pathways (Notch signaling, melanogenesis, and tyrosine metabolism) were identified in quail iris tissue (P < 0.05). The expression levels of solute carrier family 45 member 2 (SLC45A2), tyrosinase-related protein 1, vitamin D receptor, opsin 5, and docking protein 5 were significantly different between Beijing white and Chinese yellow quail, as verified by RT-qPCR. The c.1061C>T mutation in SLC45A2, which caused a single amino acid change at position 354 (threonine to methionine), was significantly associated with iris color in Beijing white and Chinese yellow quail, and might be the main reason for the different iris colors between these two quail species.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"61 ","pages":"2024015"},"PeriodicalIF":1.5,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11130394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Amino Acid Supplementation to a Low-Protein Diet on the Growth Performance and Protein Metabolism-related Factors in Broiler Chicks. 低蛋白日粮中添加氨基酸对肉鸡生长性能和蛋白质代谢相关因子的影响

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2024-05-09 eCollection Date: 2024-01-01 DOI: 10.2141/jpsa.2024014

Asmaa S El-Far, Maho Kamiya, Takaoki Saneyasu, Kazuhisa Honda

{"title":"Effects of Amino Acid Supplementation to a Low-Protein Diet on the Growth Performance and Protein Metabolism-related Factors in Broiler Chicks.","authors":"Asmaa S El-Far, Maho Kamiya, Takaoki Saneyasu, Kazuhisa Honda","doi":"10.2141/jpsa.2024014","DOIUrl":"10.2141/jpsa.2024014","url":null,"abstract":"A low-protein (LP) diet may alleviate the environmental impact of chicken meat production by reducing nitrogen excretion and ammonia emissions. Thus, this study investigated the effect of a 15% reduced protein diet with or without amino acid (AA) supplementation on the growth performance of broiler chicks from 10 to 35 days of age and the underlying mechanism for loss of skeletal muscle mass. Thirty-six male broiler chicks were allocated to three experimental groups based on body weight: control, LP, and essential AA-supplemented LP (LP+AA). The body weight gain, feed conversion ratio, and weight of breast muscles and legs significantly decreased only in the LP group at the end of the feeding period. Plasma uric acid levels were significantly lower in the LP+AA group than those of the other groups. In the LP group, mRNA levels of microtubule-associated protein 1 light chain 3 isoform B were significantly higher in the pectoralis major, whereas those of atrogin-1, muscle RING-finger protein-1, and myoblast determination protein 1 were significantly higher in the biceps femoris compared to those in the control group. There were no significant differences in insulin-like growth factor 1 mRNA levels in the liver or skeletal muscle between groups. These findings suggested that supplementation with essential AAs ameliorated the impaired effects of an LP diet on growth performance in broiler chicks, and that the transcriptional changes in proteolytic genes in skeletal muscles might be related to the impaired effects of the LP diet.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"61 ","pages":"2024014"},"PeriodicalIF":1.5,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11074001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of Fluoroquinolone-Resistant Escherichia coli in Parent Flocks Treated with Fluoroquinolones on Chick Stage and their Broiler Offspring. 在雏鸡阶段使用过氟喹诺酮类药物的母鸡群及其肉鸡后代中耐药大肠埃希氏菌的分布。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2024-04-27 eCollection Date: 2024-01-01 DOI: 10.2141/jpsa.2024013

Takeshi Kawasaki

{"title":"Distribution of Fluoroquinolone-Resistant Escherichia coli in Parent Flocks Treated with Fluoroquinolones on Chick Stage and their Broiler Offspring.","authors":"Takeshi Kawasaki","doi":"10.2141/jpsa.2024013","DOIUrl":"https://doi.org/10.2141/jpsa.2024013","url":null,"abstract":"This study investigated the distribution of ofloxacin-resistant Escherichia coli (OFXR-EC) in broiler parent flocks (PS) treated with ofloxacin for 5 days from the time the chicks arrived at the poultry house, and their broiler offspring. OFXR-EC was detected in 22.95% of neonatal parent stock chicks (PSN) arriving at the poultry house. The detection rate of OFXR-EC in PS rearing was 72.49%, which was significantly higher than that detected in PSN. In addition, the detection rate of OFXR-EC was significantly lower in neonatal chicks of their offspring broilers (CSN) at 7.06% than that of PS, but was 24.62% in offspring broiler flocks (CS) at approximately 6 weeks of age. The OFXR-EC detection rate was significantly higher in CS than that in CSN, even though no therapeutic antimicrobials, including ofloxacin, were used from CSN to CS. In addition, the proportions of OFXR-ECs in E. coli isolated from samples in which OFXR-ECs were detected were 63.85% for PSN, 10.52% for PS, 62.00% for CSN, and 8.25% for CS. There was little difference in the composition ratio of OFXR-EC between PSN and CSN, or between PS and CS.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"61 ","pages":"2024013"},"PeriodicalIF":1.5,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11043624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140874683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periodic and Local LED Light Switching Induces Broiler Locomotion. 周期性和局部 LED 灯光切换可诱导肉鸡运动

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2024-03-31 eCollection Date: 2024-01-01 DOI: 10.2141/jpsa.2024009

Shingo Kusuda, Chikamitsu Oshima, Takaoki Saneyasu, Kazuhisa Honda

{"title":"Periodic and Local LED Light Switching Induces Broiler Locomotion.","authors":"Shingo Kusuda, Chikamitsu Oshima, Takaoki Saneyasu, Kazuhisa Honda","doi":"10.2141/jpsa.2024009","DOIUrl":"10.2141/jpsa.2024009","url":null,"abstract":"Wooden breast myopathy and leg weakness are serious problems in the broiler chicken industry. The color and intensity of light in the chicken habitat affect behavior, including walking of chicks. The present study was conducted to determine whether periodic and local light switching induces locomotion and affects wooden breast myopathy and leg weakness in broiler chicks. Thirty five-day-old broiler chicks were assigned to two pens (4.72 m × 0.73 m each). In the control pen, chicks were reared under three white-light emitting diode (LED) lights until they were 42 days old. In the other pen, chicks were reared under a white LED light located in the center, supplemented with blue or red LED lights on either side of the pen. The color of the LED lights changed every 3 h, from blue and red to red and blue. From 21 d of age, all LED lights were changed and only one of the side lights was turned on every 3 h. From 35 d of age, all three white lights were turned on until 42 d of age. Periodic and local color switching and on-off switching significantly induced locomotion in broiler chicks. Wooden breast scores tended to improve with light-switching treatment. The tibia length, diameter, and breaking strength were not significantly affected. This is the first report showing that locomotion may be induced in broiler chicks by periodic and local lighting switching, and may be useful for improving the health status of broiler chicks.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"61 ","pages":"2024009"},"PeriodicalIF":1.5,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10978436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140336106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innate Immune Training in Chickens for Improved Defense against Pathogens: A Review. 鸡的先天性免疫训练可提高对病原体的防御能力：综述。

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2024-03-13 eCollection Date: 2024-01-01 DOI: 10.2141/jpsa.2024008

Yukinori Yoshimura, Takahiro Nii, Naoki Isobe

引用次数: 0

Effects of Dietary Brown Rice and Sake Lees on the Growth Performance and Color of Meat in Broiler Chicks. 糙米和酒糟对肉鸡生长性能和肉色的影响

IF 1.5 4区农林科学

Journal of Poultry Science Pub Date : 2024-02-29 eCollection Date: 2024-01-01 DOI: 10.2141/jpsa.2024007

Koki Nishikawa, Karin Miyazaki, Takehiro Hirai, Takaoki Saneyasu, Kazuhisa Honda

{"title":"Effects of Dietary Brown Rice and Sake Lees on the Growth Performance and Color of Meat in Broiler Chicks.","authors":"Koki Nishikawa, Karin Miyazaki, Takehiro Hirai, Takaoki Saneyasu, Kazuhisa Honda","doi":"10.2141/jpsa.2024007","DOIUrl":"10.2141/jpsa.2024007","url":null,"abstract":"In this study, we examined whether brown rice and sake lees (domestic feed ingredients) could replace corn and soybean meal (major imported feed ingredients) in broiler chick feed. In Experiment 1, 21-day-old broiler chicks were assigned to two groups and fed a corn-soybean- or a brown rice-soybean-based diet for three weeks (3 birds × 4 replicates/group). Dietary brown rice significantly improved body weight gain and feed conversion ratio. Brown rice feeding also significantly increased L* (lightness) in the thigh and significantly decreased a* (redness) and b* (yellowness) in the thigh and b* in the fat. In Experiment 2, 21-day-old broiler chicks were assigned to three groups and fed either a corn-soybean-based diet for 3 weeks, a corn-soybean-based diet for the first 2 weeks followed by a brown rice sake lees-based diet for the last week, or a brown rice sake lees-based diet for 3 weeks (3 birds × 4 replicates/group). Replacement of the imported feed ingredients significantly improved the feed conversion ratio. The a* values for the breast, thigh, and fat, and the b* values for the thigh and fat were significantly decreased by rice and sake lees feeding for 3 weeks. The a* values for the breasts and fat were significantly decreased by rice and sake lees feeding for 1 week. These results suggest that brown rice and sake lees can be used as replacements for imported feed ingredients such as corn and soybean meal in broiler chicks without detrimental effects on growth performance. These domestic feed ingredients may benefit local production and consumption of poultry in Japan.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"61 ","pages":"2024007"},"PeriodicalIF":1.5,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10898942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139996554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Relaxin Family Peptide Receptors 1 and 3 in the Ovarian Follicle of Japanese Quail. 日本鹌鹑卵泡中松弛素家族肽受体 1 和 3 的表达

IF 1.8 4区农林科学

Journal of Poultry Science Pub Date : 2024-02-03 eCollection Date: 2024-01-01 DOI: 10.2141/jpsa.2024005

Khoi X Hoang, Mei Matsuzaki, Tetsuya Kohsaka, Tomohiro Sasanami

{"title":"Expression of Relaxin Family Peptide Receptors 1 and 3 in the Ovarian Follicle of Japanese Quail.","authors":"Khoi X Hoang, Mei Matsuzaki, Tetsuya Kohsaka, Tomohiro Sasanami","doi":"10.2141/jpsa.2024005","DOIUrl":"10.2141/jpsa.2024005","url":null,"abstract":"In our previous studies, we demonstrated that the primary source of relaxin 3 (RLN3) in Japanese quail is ovarian granulosa cells. Although several relaxin family peptide (RXFP) receptors have been sequenced, the intricacies of these receptors in avian species remain insufficiently clarified. Therefore, we assessed the expression of RXFP receptors, RXFP1 and 3, in Japanese quail. Using RT-PCR, we found that both RXFP1 and 3 were ubiquitously expressed. The expression level of RXFP1 is significantly higher in the ovarian theca layer, indicating that it is the primary receptor for RLN3 in the ovary. During follicular development, there was an elevation in thecal RXFP1 expression, but it declined after the luteinizing hormone (LH) surge. We found that the protease activity of the 60 kDa band increased after the LH surge, suggesting the involvement of RLN3 signaling in ovulation. These results suggest a paracrine role of RLN3, involving its binding with RXFP1 in ovarian theca cells. This interaction may elicit biological actions, potentially initiating ovulation after the LH surge.","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"61 ","pages":"2024005"},"PeriodicalIF":1.8,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10830672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139681293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0