Journal of Poultry Science最新文献

{"title":"Effects of <i>Phellinus linteus</i>-Fermented Wheat Bran on Growth Performance, Intestinal Morphology, and Modulation of Nrf2-ARE-Associated Gene Expression of Broilers during Summer Conditions.","authors":"Tzu Hsiang Wei, Shen Chang Chang, Jhih Siang Chang, Min Jung Lin, Li Jen Lin, Tzu Tai Lee","doi":"10.2141/jpsa.2026013","DOIUrl":"10.2141/jpsa.2026013","url":null,"abstract":"<p><p>This study investigated the effects of <i>Phellinus linteus</i>-fermented wheat bran (FWB) on growth performance, intestinal morphology, and modulation of nuclear factor erythroid 2-related factor 2-antioxidant response element-associated gene expression in broilers during summer conditions. The fiber content and cellulolytic enzyme activity of wheat bran (WB) improved after fermentation. In addition, FWB had a significantly higher secondary metabolite content, such as crude polysaccharides, ergosterol, and crude triterpenoids. Moreover, FWB showed 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging and ferrous ion-chelating abilities. Three hundred 1-day-old broiler chickens (Ross 308) were assigned into five groups fed the control diet or the control diet replaced with 5% WB, 10% WB, 5% FWB or 10% FWB. Over the entire experimental period, broilers in the 5% FWB group had a significantly lower feed conversion ratio than that of broilers in the control group. Assessment of blood characteristics revealed that broilers fed FWB had significantly higher serum superoxide dismutase activity and reduced malondialdehyde production. Measurement of the expression of antioxidant-related genes and proinflammatory cytokines in broiler peripheral blood mononuclear cells showed that partially replacing FWB in broiler diets enhanced the expression of antioxidant-related genes and decreased the expression of proinflammatory cytokines. These results indicated that <i>P. linteus</i> increased the feed value of WB and improved the growth performance and antioxidant capacity of broiler chickens, suggesting its potential for application in broiler diets during summer conditions.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"63 ","pages":"2026013"},"PeriodicalIF":2.1,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13294592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny and Genetic Diversity of Philippine Mallard Ducks Based on the Mitochondrial DNA D-loop Region.","authors":"Cyrill John P Godinez, Erik John S Putan, Carla M Cabardo, Leonard Andrew C Ceballos, Cloi D Adolfo, Justine Bennette H Millado, Masae Hirose, Masahide Nishibori","doi":"10.2141/jpsa.2026012","DOIUrl":"10.2141/jpsa.2026012","url":null,"abstract":"<p><p>As with other locally adapted species, Philippine mallard ducks risk population fragmentation and indiscriminate hybridization. To allow for targeted breed improvement, this study aimed to unravel the maternal genetic relationships, phylogeny, and levels of genetic diversity in Philippine mallard ducks. We sequenced the mitochondrial DNA D-loop region of 118 ducks sampled from the Southern Leyte, Samar, and Cebu Provinces. Sequence data analysis revealed nine transition base substitutions and eight distinct haplotypes. Overall haplotypic diversity (<i>Hd</i> = 0.666 ± 0.038) exceeded that reported for Javanese ducks, but was comparable to that of Thai, Chinese, and Indian domestic duck populations. The matrilineal phylogenetic tree positioned all Philippine mallard ducks within the Old-World Haplogroup A. Specifically, they clustered into subhaplogroup A3 (75/118), together with domestic ducks from China and Southeast Asia; putative ancestral subhaplogroups A0 (6/118) and A1a (5/118) in a basal position, showing close genetic affinities with Eurasian wild mallards and Eastern spot-billed ducks; and subhaplogroup A1b (6/118), showing genetic relatedness to the Indian Runner duck. Notably, 26 individuals formed a distinct cluster corresponding to subhaplogroup A2, which appeared to be unique to this population. This study provides the first mitochondrial DNA D-loop-based genetic characterization of Philippine mallard ducks, placing this population within the broader Haplogroup A lineage and highlighting how its genetics was shaped by island biogeography. Collectively, these results establish the basis for future research on animal genetic resources and phenomics, supporting the sustainable management of mallard duck populations in the Philippines.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"63 ","pages":"2026012"},"PeriodicalIF":2.1,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13250243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148251775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraperitoneal Injection of Cisplatin Induces Nausea-Like Responses without Vomiting in Chicks (<i>Gallus gallus domesticus</i>).","authors":"Kaoru Nakamura, Sakirul Khan, Mark A Cline, Tetsuya Tachibana","doi":"10.2141/jpsa.2026011","DOIUrl":"10.2141/jpsa.2026011","url":null,"abstract":"<p><p>Cisplatin is an anti-cancer drug that causes nausea and vomiting as adverse effects. Cisplatin-induced responses have been widely used to investigate the mechanisms underlying nausea and vomiting in several mammalian species. Because nausea and vomiting are caused by a variety of factors, including disease and stress, clarification of these mechanisms would contribute to improving animal welfare in poultry production. However, the effects of cisplatin in chickens have not been well documented. Thus, we examined whether intraperitoneal (IP) injection of cisplatin caused nausea and vomiting in chicks. IP injection of cisplatin decreased food intake, voluntary activity, and the passage of food from the crop. Moreover, cisplatin tended to increase plasma corticosterone concentrations and decreased plasma glucose concentration. As these effects are consistent with nausea-related responses observed in mammals, cisplatin might also induce nausea in chicks. However, cisplatin did not induce vomiting in any experiments. Furthermore, cisplatin slightly decreased the pH of the crop contents and phenol red gavaged into the proventriculus refluxed into the crop, although these effects were not considerable. Thus, the lack of substantial reflux from the proventriculus prevented vomiting in chicks after cisplatin injection.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"63 ","pages":"2026011"},"PeriodicalIF":2.1,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13230502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of Fibro-Adipogenic Progenitors and Cellular Communication Network Family Signaling in the Impaired Muscle Regeneration of Wooden Breast.","authors":"Ryosuke Kobayashi, Marina Hosotani, Karin Saito, Yukitaka Masuda, Takeshi Kawasaki, Naoki Takahashi, Yasuhiro Hasegawa, Tomohito Iwasaki, Takafumi Watanabe","doi":"10.2141/jpsa.2026009","DOIUrl":"10.2141/jpsa.2026009","url":null,"abstract":"<p><p>Wooden Breast is a myopathy affecting broiler chickens, characterized by hardening of the pectoral muscle, fibrosis, adipogenesis, and deteriorated meat quality. This study investigated the pathological mechanisms underlying wooden breast development, focusing on the interplay between fibrogenesis and myogenesis. Seventy-eight broiler chickens were categorized into normal, mild, and severe wooden breast groups based on the extent of fibrosis and adipogenesis in the pectoral muscle. Histological analysis revealed immature collagen fibers within muscle fascicles in severe wooden breast, indicating concurrent fibrogenesis and muscle regeneration. Immunofluorescence staining confirmed the close spatial localization of fibro-adipogenic progenitors (FAPs) and myosatellite cells in these areas, suggesting potential interaction during wooden breast pathogenesis. RNA sequencing and qPCR revealed upregulation of <i>PAX7</i> and <i>MYOG</i>, markers of myogenesis, in the affected pectoral muscle, whereas <i>MYOD</i> expression remained unchanged. This pattern indicates an attempt at myogenic differentiation that is ultimately disrupted in severe wooden breast samples. Furthermore, cellular communication network (CCN) family members, particularly <i>CCN2</i> and <i>CCN4</i>, were upregulated in the wooden breast-affected pectoral muscle. <i>CCN4</i> expression strongly correlated with the fibro-adipogenic progenitor marker <i>PDGFRα</i>, implying that fibro-adipogenic progenitors-mediated <i>CCN4</i> secretion contributes to wooden breast pathogenesis. Our findings suggest that fibro-adipogenic progenitors and cellular communication network family members are associated with an imbalance between fibrogenesis and myogenesis, leading to the muscle degeneration observed in wooden breast.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"63 ","pages":"2026009"},"PeriodicalIF":2.1,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13099151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementation of 5-Aminolevulinic Acid Suppressed Body Weight Loss and Reduced Disease Severity During <i>Eimeria tenella</i> Infection in Broiler Chickens.","authors":"Taqi Ahmad Hanif, Makoto Matsubayashi, Toshimitsu Hatabu","doi":"10.2141/jpsa.2026010","DOIUrl":"10.2141/jpsa.2026010","url":null,"abstract":"<p><p>This study aimed to evaluate the effects of 5-aminolevulinic acid (5-ALA) supplementation in broiler chickens infected with <i>Eimeria tenella</i>. To assess these effects, chickens supplemented with 20 ppm 5-ALA (5-ALA group) were compared with non-supplemented controls (control group). Sporulated <i>E. tenella</i> oocysts (2.0 × 10³ oocysts per animal) were administered orally to 2-week-old broiler chickens. Body weight was measured weekly, and fecal samples were collected daily from 4 to 15 days post-infection (dpi). Fecal oocyst shedding was quantified using the sucrose flotation method. Cecal tissues were collected at 5 dpi for histopathological analysis and lesion scoring. The animals in the 5-ALA group exhibited significantly greater weight gain and milder clinical signs than those in the control group. Fecal oocyst shedding was highest at 7 dpi in both groups; however, the 5-ALA group exhibited significantly lower oocyst output than the control group. The total number of fecal oocysts shed during the acute infection period was significantly lower in the 5-ALA group than in the control group. Histopathological analysis revealed that although both groups exhibited epithelial hyperplasia and <i>E. tenella</i> schizonts in the cecal submucosa, inflammatory cell infiltration, cecal tissue damage, and histological lesion scores were significantly lower in the 5-ALA group than in the control group. These results suggest that 5-ALA supplementation may mitigate the clinical, parasitological, and histological effects of <i>E. tenella</i> infection in broiler chickens.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"63 ","pages":"2026010"},"PeriodicalIF":2.1,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13101652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Dietary Supplementation with Methyl Salicylate on Performance, Blood Biochemistry, Intestinal Morphology, and Meat Quality of White-Feather Broilers.","authors":"Jiachen Li, Yanfei Ma, Jiangdi Mao, Wenjie Tang, Maolong He, Yongxia Wang, Haifeng Wang","doi":"10.2141/jpsa.2026008","DOIUrl":"10.2141/jpsa.2026008","url":null,"abstract":"<p><p>This study aimed to enhance meat quality and intestinal health of white-feathered broilers by utilizing methyl salicylate (MS), a fragrant plant-derived essential oil, for its anti-inflammatory, antibacterial, and antiplatelet properties. Specifically, we investigated the effects of dietary MS supplementation on the growth performance, carcass characteristics, intestinal morphology, and blood biochemistry and breast meat quality. A total of 270 one-day-old white-feather broilers were randomly assigned to three dietary treatments: a basal diet (CON), a basal diet supplemented with methyl salicylate at a low dosage of 0.25 g/kg (MSL), or at high dosage of 0.5 g/kg (MSH). Dietary MS treatment did not significantly affect the growth performance of broilers. However, dietary methyl salicylate supplementation significantly increased the blood levels of high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total cholesterol (Tchol), and total antioxidant capacity (T-AOC) (<i>P <</i> 0.05). High-dose methyl salicylate supplementation also increased villus height in the jejunum (<i>P</i> < 0.05). Furthermore, methyl salicylate supplementation improved the a* value (redness) of breast muscle (<i>P</i> < 0.05). Additionally, the addition of methyl salicylate supplementation significantly retarded the increase in thiobarbituric acid (TBA) and total volatile basic nitrogen (TVB-N) levels in breast muscle (<i>P</i> < 0.05), potentially benefiting long-term meat preservation. Moreover, methyl salicylate increased the content of monounsaturated fatty acids in breast meat (<i>P</i> < 0.05). Consequently, our findings indicate that dietary supplementation with MS enhances meat quality and may extend the shelf life of white-feathered broilers by improving blood lipid parameters and modifying muscle fatty acid profiles.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"63 ","pages":"2026008"},"PeriodicalIF":2.1,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13087912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147729349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucose Intake during the Neonatal Period Modulates the Central Melanocortin System with Breed-Specific Differences.","authors":"Kazuki Kimura, Yuuna Watanabe, Sachi Ashizawa, Koichi Matsushita, Nobuhiro Nakao, Yoshiyuki Ohta, Jun-Ichi Shiraishi","doi":"10.2141/jpsa.2026007","DOIUrl":"10.2141/jpsa.2026007","url":null,"abstract":"<p><p>Glucose is the primary substrate for cellular energy production, and its blood concentration is tightly regulated. This study investigated the effect of glucose supplementation during the first three days post-hatching on growth performance and diencephalic melanocortin-related gene expression in Red Cornish (RC) and Yamanashi Oh-Shamo (OSH-Y) chicks. We observed that body weight and weight gain were significantly higher in RC than in OSH-Y chicks. Glucose administration reduced body weight and feed intake at three days of age although no differences persisted thereafter. Significant differences in the expression of <i>agouti-related peptide</i>, <i>AMP-activated protein kinase α2, cholecystokinin A receptor</i>, and <i>insulin receptor</i> genes were detected between the two breeds. Conversely, <i>neuropeptide Y</i> was significantly downregulated by glucose supplementation in both breeds. <i>Melanocortin receptor subtypes 3, 4,</i> and <i>melanocortin receptor accessory protein 2</i> exhibited significant breed and treatment interactions, with higher expression detected in glucose-fed OSH-Y chicks. These findings indicate that newly hatched chicks possess a centrally mediated glucose-sensing system that transiently suppresses feed intake. The more pronounced response observed in OSH-Y chicks suggests heightened hypothalamic glucose sensitivity, possibly mediated by the activation of melanocortin receptor signaling.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"63 ","pages":"2026007"},"PeriodicalIF":2.1,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12961303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147377836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dampwood Termite <i>Hodotermopsis sjostedti</i> as a Dietary Source for Broiler Chickens.","authors":"Yoko Tsukahara, Nami Tomonaga, Eisuke Tasaki, Yuki Mitaka, Shozo Tomonaga, Hajime Kumagai, Hiroyuki Hirooka, Kenji Matsuura","doi":"10.2141/jpsa.2026006","DOIUrl":"10.2141/jpsa.2026006","url":null,"abstract":"<p><p>Insects are commanding increasing attention as a sustainable and alternative nutritional source in animal feed. In this study, the safety and nutritional profile of the dampwood termite, <i>Hodotermopsis sjostedti,</i> were evaluated as a novel ingredient in chicken feed. Colonies were lab-reared, and individual termites were collected by carefully crushing the wood, freeze-drying, and grinding. The resulting termite meal contained 65.6% crude protein, 16.0% fat, and 5.2% ash, on a dry matter basis. No minerals were present at toxic concentrations. Two feeding experiments using Ross 308 female broilers were conducted to evaluate the effects of termite meal inclusion. In Experiment 1, 20 chickens (8-d-old) were assigned to four treatments consisting of a commercial diet plus: 5.0% fish meal, 4.5% fish meal + 0.5% termite meal, 2.5% fish meal + 2.5% termite meal, and 5.0% termite meal. In Experiment 2, 18 chickens (9-d-old) were assigned to three diets: control, control + 2.5% termite meal, and control + 2.5% fish meal. Body weight and feed intake were recorded daily, and samples were collected at 21 d of age. Growth performances, organ weights, and blood plasma profiles were generally comparable across treatments. Chickens receiving 5.0% termite meal had greater alanine aminotransferase values, suggesting a potential hepatic burden. No adverse effects on growth, meat, or organ weight were observed. In conclusion, <i>H. sjostedti</i> meal demonstrated strong potential as a novel dietary component for broiler chickens. However, further long-term studies with larger numbers of birds are necessary to ensure a safe and effective use in poultry diets.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"63 ","pages":"2026006"},"PeriodicalIF":1.6,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12890253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146180701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral Administration of Hypertonic Saline Affects Nutrient Metabolism-related Gene Expression in Chicks.","authors":"Kaoruko Murata, Nnamdi Godswill Dialoke, Yuhui Zhang, Junya Takegaki, Takaoki Saneyasu, Kazuhisa Honda","doi":"10.2141/jpsa.2026004","DOIUrl":"10.2141/jpsa.2026004","url":null,"abstract":"<p><p>When facing dehydration, birds use body fat and proteins as a source of endogenous water. Recently, we found that osmotic stress triggered protein catabolism in skeletal muscles of chicks. In the present study, we investigated whether osmotic stress also affected fat metabolism. Twelve 21-day-old male chicks were allocated to two groups based on body weight. The chicks received either saline (0.15 M NaCl) or hypertonic saline (1.5 M NaCl) intraperitoneally (5 mL/kg body weight). After 1 h of feed and water deprivation, the chicks were euthanized by decapitation. Blood, breast muscle (<i>pectoralis major</i>), abdominal adipose tissue, liver, and kidney samples were collected, followed by analysis of plasma components and gene expression. Hypertonic saline significantly increased the plasma levels of non-esterified fatty acids (NEFA); adipose triglyceride lipase (ATGL) mRNA in adipose tissue; pyruvate dehydrogenase kinase 4 (PDK4) mRNA in breast muscle, adipose tissue, and liver; phosphoenolpyruvate carboxykinase 1 mRNA in the liver; and atrogin-1 mRNA in breast muscle. It also increased the plasma content of the hormone vasotocin. Subsequent intravascular administration of vasotocin significantly elevated plasma NEFA, but not ATGL or PDK4 mRNA in adipose tissue. Collectively, our findings suggest that osmotic stress alters nutrient metabolism in the peripheral tissues of chicks and that vasotocin may be partly involved in fatty acid mobilization.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"63 ","pages":"2026004"},"PeriodicalIF":2.1,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12803757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>MAP3K7CL</i> Inhibits the Inflammatory Responses in Goose Fatty Liver.","authors":"Minmeng Zhao, Mengqing Lv, Jiahui Li, Xiang Fan, Long Liu, Daoqing Gong, Yihui Zhang","doi":"10.2141/jpsa.2026005","DOIUrl":"10.2141/jpsa.2026005","url":null,"abstract":"<p><p>Inflammation often accompanies the development of liver diseases in humans, but appears to be repressed in geese. This study investigated the role of MAP3K7 C-terminal-like (<i>MAP3K7CL</i>) in goose fatty liver formation. Sixteen healthy 70-day-old male geese were randomly divided into control and overfed groups. Additionally, the transcriptome analysis after <i>MAP3K7CL</i> overexpression and lipopolysaccharide (LPS) treatment were performed in goose primary hepatocytes. The results showed that the <i>MAP3K7CL</i> mRNA expression was increased in the liver of overfed treatment compared to control group. Overexpression of <i>MAP3K7CL</i> in primary goose hepatocytes identified differentially expressed genes enriched in the mitogen-activated protein kinase (MAPK) signaling pathway. Specifically, DNA damage-inducible transcript 3 (<i>DDIT3</i>), insulin-like growth factor 1 receptor (<i>IGF1R</i>), neurofibromin 1 (<i>NF1</i>), and platelet-derived growth factor subunit B (<i>PDGFB</i>) were significantly downregulated upon <i>MAP3K7CL</i> overexpression, whereas heat shock protein family B member 1 (<i>HSPB1</i>) was significantly upregulated. Furthermore, transfection of goose hepatocytes with the <i>MAP3K7CL</i> overexpression vector lowered the expression of lipopolysaccharide-induced TNF factor (<i>LITAF</i>) and cysteinyl aspartate-specific proteinase-3, which are associated with inflammation and apoptosis, respectively. In accordance with these findings, <i>DDIT3</i> and <i>LITAF</i> were downregulated in the overfed group, whereas <i>HSPB1</i> was upregulated. Compared with the control, LPS treatment significantly decreased <i>MAP3K7CL</i> expression, while promoting that of <i>LITAF</i> and interleukin-6 (<i>IL-6</i>). Moreover, the combination of lipopolysaccharide and <i>MAP3K7CL</i> overexpression upregulated <i>MAP3K7CL</i> while downregulating <i>LITAF</i> and <i>IL-6</i> with respect to LPS alone and empty vector control groups. Therefore, <i>MAP3K7CL</i> may inhibit the inflammatory response in goose fatty liver.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"63 ","pages":"2026005"},"PeriodicalIF":2.1,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12803758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}