Journal of Phytopathology最新文献

{"title":"Efficacy of Betel Leaf Botanical Pesticide Against Fusarium Wilt Disease (Fusarium oxysporum f.sp capsici) in Chilli Plants","authors":"Ida Yusidah,&nbsp;Salamet Ginandjar,&nbsp;Rina Budi,&nbsp;Ifah Shofiyyatul Mi'rajiyyah","doi":"10.1111/jph.70355","DOIUrl":"https://doi.org/10.1111/jph.70355","url":null,"abstract":"<div>\u0000 \u0000 <p>Fusarium wilt cased by <i>Fusarium oxysporum</i> f.sp. <i>capsici</i> is a major and aggressive disease, responsible for significant losses of the chilli production worldwide. An environmentally friendly control alternative is needed to overcome Fusarium wilt, namely by applying botanical pesticides from betel leaves, which is expected to minimize the negative impacts of synthetic chemical pesticides. The purpose of this study were to determine the effect of betel leaves botanical pesticide concentrations on the growth of <i>F. oxysporum</i> f.sp. <i>capsici</i> and the development of Fusarium wilt disease in chilli plants. This study was conducted at the Center for Plant Pest Forecasting (BBPOPT) Karawang, West Java. A Completely Randomized Design (CRD) was used for in vitro testing and a Randomized Block Design (RBD) for in vivo testing. Both experiments tested six concentrations: 0% (control), 10%, 20%, 30%, 40%, 50%, each replicated four times. The Duncan's Multiple Range Test (DMRT) was used for further testing. The results showed that the application of betel leaf botanical pesticide significantly affected to the growth of <i>F. oxysporum</i> f.sp. <i>capsici</i>, disease intensity, chilli plant height and fresh fruit weight with the 50% betel leaf botanical pesticide being the best concentration. The mechanism of betel leaf botanical pesticide inhibiting Fusarium wilt disease occurs both directly and indirectly by active compounds (phytoalexins) of betel leaves. Directly, they are able to damage the fungal cell wall and inhibit the growth of spores. Indirectly, they are able to stimulate the plant defence system through gene signalling pathways and lnRNA. The betel leaf botanical pesticides can as solution for sustainable agriculture and green development strategies, because it's capable of helping manage pest resistance and generally safe for the environment.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Leaf Mould of Spinach Caused by Curvularia hominis in China","authors":"Yanqing Li,&nbsp;Xiugao Zhu,&nbsp;Cuicui Wang,&nbsp;Lin Cheng,&nbsp;Haoqin Pan","doi":"10.1111/jph.70362","DOIUrl":"https://doi.org/10.1111/jph.70362","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Spinacia oleracea</i> L. is a major vegetable crop in China, accounting for 70% of the global planting area. In October 2024, leaf mould disease with a 65% incidence was observed on spinach in greenhouses in Niutou Town, Weifang, Shandong Province. The lower leaves turn yellow first then developed into white spots; they were circular to nearly circular, ranging from 0.3 to 20 mm in size. Through experiments, fourteen fungal strains were isolated. Their colony and conidium morphology matched the genus <i>Curvularia</i>. Multilocus sequence analysis (tef1α, ITS, LSU) showed 99.26%–99.82% identity to <i>Curvularia hominis</i>, and phylogenetic trees clustered the isolates with <i>C. hominis</i>. Pathogenicity tests confirmed that the representative strain Bocai7 induced typical symptoms, and the pathogen was reisolated successfully, fulfilling Koch's postulates. This is the first report of <i>C. hominis</i> causing spinach leaf mould, which threatens spinach yield and quality by impairing photosynthesis and nutrient transport.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Erysiphe euonymicola Causing Powdery Mildew on Euonymus japonicus in Brazil","authors":"Leandro Jose Dallagnol,&nbsp;Sabrina de Oliveira Martins,&nbsp;Fernanda Rezende de Castro-Moretti,&nbsp;Jeronimo Vieira de Araujo Filho,&nbsp;Luis Eduardo Aranha Camargo","doi":"10.1111/jph.70361","DOIUrl":"https://doi.org/10.1111/jph.70361","url":null,"abstract":"<div>\u0000 \u0000 <p>Plants of <i>Euonymus japonicus</i> (Celastraceae), an ornamental shrub, exhibiting powdery mildew symptoms were collected in Pelotas, state of Rio Grande do Sul, Brazil. Based on morphological characteristics, including conidia, conidiophore, and appressoria, as well as analysis of the internal transcribed spacer (ITS1-5.8 s-ITS2) region, the causal agent was identified as <i>Erysiphe euonymicola</i>. Pathogenicity was confirmed by inoculating five healthy branches of <i>E. japonicus</i> with conidia collected from symptomatic leaves. Typical powdery mildew symptoms developed 10–12 days after inoculation only on fungal inoculated branches, whereas control plants remained asymptomatic. To the best of our knowledge, this is the first record of <i>E. euonymicola</i> infecting <i>E. japonicus</i> in Brazil.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148466918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Putative Spondias Leaf Extracts Against Macrophomina spp. in Melon Plants","authors":"Sarah Sanny de Lima Peixôto,&nbsp;Andréia Mitsa Paiva Negreiros,&nbsp;Ismaele Breckenfeld da Costa,&nbsp;Pedro Sidarque Lima Pinto,&nbsp;Letícia Samilly de Lima Peixôto,&nbsp;Maria de Fátima Duarte de Souza Neta,&nbsp;Fernanda Inácio de Lima,&nbsp;Ioná Santos Araújo Holanda","doi":"10.1111/jph.70349","DOIUrl":"https://doi.org/10.1111/jph.70349","url":null,"abstract":"<div>\u0000 \u0000 <p>Fungi of the genus <i>Macrophomina</i> are widely distributed necrotrophic pathogens associated with various agricultural crops, including charcoal rot of melon. The limited effectiveness of chemical and genetic control strategies, combined with the high survival of the pathogen in soil and the absence of resistant cultivars, highlights the need for more sustainable management strategies. In this context, this study aimed to evaluate the antifungal potential of leaf extracts from <i>Spondias</i> species against <i>Macrophomina phaseolina, Macrophomina pseudophaseolina</i> and <i>Macrophomina euphorbiicola</i>, under in vitro and in vivo in melon plants. Ethanolic extracts (70%) were obtained from leaves of five <i>Spondias</i> genotypes (UMBU, CAJ-52, CAJ-53, SERI-60 and SERI-64). The in vitro assay was conducted using the disk diffusion method, at concentrations of 0%–50%, evaluating the percentage of mycelial growth inhibition (MGI) and determining the effective concentration (EC₅₀). In vivo assays were performed in a greenhouse using ‘Natal’ RZ F1 melon seeds grown in infested soil, with weekly applications of the extracts (15%). Forty days after sowing, disease incidence and severity, as well as biometric variables, were evaluated. The in vitro results showed that all extracts caused 100% inhibition of mycelial growth at a concentration of 15%. Under in vivo conditions, CAJ-53, CAJ-52 and SERI-64 extracts showed greater efficiency in controlling <i>M. phaseolina</i>, <i>M</i>. <i>pseudophaseolina</i> and <i>M. euphorbiicola</i>, with severity reductions of 53%, 65% and 64%, respectively, compared to the positive control. Overall, leaf extracts of <i>Spondias</i> spp. inhibited the growth of <i>Macrophomina</i> spp. and reduced disease severity, highlighting their potential as a sustainable control alternative.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148466907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Botryosphaeria dothidea Causing Leaf Blight on Magnolia liliiflora in China","authors":"Haoqin Pan,&nbsp;Jing Zhang,&nbsp;Cuicui Wang,&nbsp;Yanqing Li,&nbsp;Wenjuan Chi,&nbsp;Yingxin Ma,&nbsp;Bo Zheng","doi":"10.1111/jph.70360","DOIUrl":"https://doi.org/10.1111/jph.70360","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Magnolia liliiflora</i> is a widely cultivated ornamental plant in China. In September 2025, a leaf blight disease was observed on <i>M. liliiflora</i> in Shandong Province. A field survey of 60 plants showed that 40% of the plants were symptomatic, with 20%–30% of leaves affected per diseased plant. A fungus was consistently isolated from symptomatic leaf tissues. Based on morphological characteristics, phylogenetic analyses of ITS, <i>TUB2</i> and <i>TEF1-α</i> gene sequences and pathogenicity tests, the isolates were identified as <i>Botryosphaeria dothidea</i>. To our knowledge, this is the first report of <i>B. dothidea</i> causing leaf blight on <i>M. liliiflora</i> worldwide, providing a foundation for future epidemiological studies and management strategies.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148466908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-Friendly Management of Viral Diseases in Cucumber (Cucumis sativus L.) Using Botanical Extracts and Milk Treatments","authors":"Pankaj Kumar Yadav,&nbsp;Sujan Lamichhane,&nbsp;Prabina Bhujel,&nbsp;Pratistha Adhikari","doi":"10.1111/jph.70348","DOIUrl":"https://doi.org/10.1111/jph.70348","url":null,"abstract":"<div>\u0000 \u0000 <p>Viral diseases, particularly those caused by Cucumber mosaic virus (CMV), are among the major constraints affecting cucumber (<i>Cucumis sativus</i> L.) production worldwide, leading to reduced plant growth, poor fruit set and significant yield losses. Considering these challenges, the present study investigated the effectiveness of selected botanical extracts and cow milk in suppressing viral disease symptoms and improving the growth and productivity of cucumber. The experiment was conducted under greenhouse conditions at Budhanilkantha, Kathmandu, Nepal, using a Randomized Complete Block Design with twelve treatments and three replications. Botanical extracts from <i>Cannabis sativa</i>, <i>Artemisia vulgaris</i>, <i>Eupatorium cannabinum</i>, <i>Tinospora cordifolia</i>, <i>Allium sativum</i>, <i>Azadirachta indica</i>, <i>Curcuma longa</i>, <i>Urtica dioica</i> and <i>Tagetes erecta</i>, along with cow milk solutions (10 and 20 mL L⁻¹), were applied as foliar sprays at regular intervals. Plant growth attributes, disease symptoms, phenological parameters and yield components were recorded at different growth stages. The results showed significant variation among treatments for all observed parameters. Cow milk at 20 mL L⁻¹ reduced AUDPC by 73% compared to control, recording the lowest Area Under Disease Progress Curve (AUDPC) and the highest number of fruits per plant (14.00). Similarly, <i>Tagetes erecta</i> extract markedly enhanced vegetative and reproductive growth, including leaf number, node formation, branching, tendril development, cluster formation and flower production. Overall, the findings suggest that cow milk and selected botanical extracts, particularly <i>Tagetes erecta</i>, offer an eco-friendly and sustainable alternative to synthetic chemicals for managing viral diseases in cucumber cultivation and improving crop productivity.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148466827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Neopestalotiopsis hispanica Associated With Strawberry Leaf Spot in Nova Scotia and Evaluation of Cultivar Resistance","authors":"Dustin MacLean,&nbsp;Jyoti Joshi,&nbsp;Tudor Borza,&nbsp;Gordon S. Murray,&nbsp;Gefu Wang-Pruski,&nbsp;Yunfei Jiang","doi":"10.1111/jph.70358","DOIUrl":"https://doi.org/10.1111/jph.70358","url":null,"abstract":"<p><i>Neopestalotiopsis</i> species have recently emerged as important pathogens of strawberry (<i>Fragaria × ananassa</i>) in several production regions; however, species-level identification of these pathogens in Atlantic Canada has not previously been reported. Field surveys conducted in Nova Scotia in July 2024 identified leaf spot symptoms in strawberry field nurseries, with a disease incidence of approximately 30%. Symptomatic leaf tissues were collected, and fungal isolates were obtained and characterized through morphological examination, pathogenicity testing, and multilocus molecular analyses. Pathogenicity was confirmed using detached-leaf and whole-plant inoculation assays, which reproduced characteristic leaf and fruit symptoms and fulfilled Koch's postulates. Species identification based on partial β-tubulin (<i>TUB2</i>), internal transcribed spacer (<i>ITS</i>) sequences, and translation elongation factor 1-alpha (<i>TEF1-α</i>) sequences, combined with concatenated phylogenetic analyses, consistently placed the isolates within <i>Neopestalotiopsis hispanica</i>. These isolates clustered closely with reference strains from diverse geographic regions and hosts. To our knowledge, this study provides the first species-level confirmation of <i>Neopestalotiopsis hispanica</i> associated with strawberry in Nova Scotia. Additionally, screening of 23 strawberry cultivars from Canadian and U.S. breeding programs revealed significant variation in disease response: Encore (‘FL 20.34-183’), K09-4, and Valley Sunset were completely resistant, whereas Annapolis, Sable, Dickens, and Kate were highly susceptible. These findings provide essential baseline information for accurate pathogen identification and support disease management strategies, including cultivar selection for regional strawberry production systems.</p>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jph.70358","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148466616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weather-Driven Epidemiology of Cercospora Leaf Spot of Mothbean Under Hot Arid Conditions","authors":"Santosh Kumari,&nbsp;Jasveer Singh,&nbsp;Data Ram Kumhar,&nbsp;Shipra Sharma,&nbsp;Anuj Anuj,&nbsp;Prdeep Singh Sekhawat,&nbsp;Ritu Mawar,&nbsp;Jayanthi Barasarathi,&nbsp;Riyaz Sayyed","doi":"10.1111/jph.70352","DOIUrl":"https://doi.org/10.1111/jph.70352","url":null,"abstract":"<div>\u0000 \u0000 <p>Cercospora leaf spot, caused by <i>Cercospora canescens</i> Ellis &amp; Martin, is a major foliar disease limiting mothbean (<i>Vigna aconitifolia</i>) production in arid and semi-arid regions of India. However, quantitative understanding of weather–disease relationships under hot arid conditions remains limited. Field experiments were conducted during the kharif seasons of 2023 and 2024 at Bikaner, Rajasthan, to investigate the temporal dynamics of disease development and its association with key meteorological variables and climate change. Disease severity was assessed weekly on three susceptible cultivars and analysed in relation to temperature, relative humidity, rainfall, number of rainy days and sunshine duration. Disease intensity increased progressively from early August, reached peak levels (40%–44% PDI) during mid-September, and declined slightly towards crop maturity, exhibiting a typical weather-driven epidemic pattern. Correlation analysis revealed that maximum temperature (<i>r</i> = 0.47, <i>p</i> &lt; 0.01) and sunshine hours (<i>r</i> = 0.53, <i>p</i> &lt; 0.001) were positively associated with disease intensity, whereas minimum temperature showed a significant negative relationship (<i>r</i> = −0.41, <i>p</i> &lt; 0.01). Multiple regression analysis explained a substantial proportion of the variability in disease (<i>R</i>² = 0.62), with minimum temperature and sunshine duration emerging as key predictors. Mixed-effects modelling further confirmed the dominant influence of temperature variables while accounting for repeated observations. Although cultivars differed in disease severity, with RMO-2251 being most susceptible and RMO-257 relatively tolerant, the overall temporal pattern remained consistent, indicating that environmental factors primarily drive epidemic progression. The study provides a robust quantitative basis for developing weather-based forecasting models for Cercospora leaf spot in mothbean under arid agro-climatic conditions.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148462815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defence Related Enzyme Activity in Rice Responses to Streptomyces spp. Biocontrol and Rhizoctonia solani Infection","authors":"Hayman Kakakhan Awla","doi":"10.1111/jph.70353","DOIUrl":"https://doi.org/10.1111/jph.70353","url":null,"abstract":"<div>\u0000 \u0000 <p>Rice cultivation is affected by a number of constraints due to fungi such as <i>Rhizoctonia solani</i>, which may cause reduction in rice grain yield up to 50%. Conventional fungicides are environmentally hazardous, making it important to look for environmentally safe alternatives such as Streptomyces. This genus of filamentous bacteria has been recognized as a biocontrol agent due to their antifungal metabolite production and induction of systemic resistance. The objective of the study was to examine the effect of <i>Streptomyces gilvosporeus</i> H1, <i>Streptomyces narbonensis</i> H2 and their combination on rice growth and defence systems against <i>R. solani</i>. The results of the study showed that although rice growth was significantly affected by the pathogen, the application of Streptomyces, especially the combination of H1 + H2, reduced the effects of Rhizoctonia. The combination gave the best results in rice productivity, as evidenced by the consortium treatment outperforming the other treatments in shoot dry weight (32.333 g), number of tillers (10.0) and 100-seed weight (1.822 g). The application of Streptomyces had a significant effect on rice defence systems. The results showed an increasing trend in the activity of the enzyme peroxidase in the treated rice. The enzyme activity reached a peak on Day 23 before declining. Moreover, these beneficial bacteria were also seen to increase the polyphenols and proteins, which were significantly reduced by the pathogen alone treatments. These results indicated that Streptomyces increases rice resilience through reinforcement of the rice cell wall fortification and antioxidant capacity. Thus, <i>Streptomyces</i> spp. are seen to be good biocontrol agents and growth promoters, providing a possible eco-friendly means of sustainable rice production.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148462839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multilocus Identification and Pathogenicity of Aspergillus tubingensis Associated With Postharvest Black Mould Rot of Apple in Türkiye","authors":"Göksel Özer,&nbsp;Muharrem Türkkan,&nbsp;Mehtap Alkan,&nbsp;Tuğba Bozoğlu,&nbsp;Sibel Derviş","doi":"10.1111/jph.70354","DOIUrl":"https://doi.org/10.1111/jph.70354","url":null,"abstract":"<div>\u0000 \u0000 <p>Black mould rot is a common postharvest disease of pome fruits, but the species identity of black aspergilli associated with these symptoms is not always resolved. In this study, two representative isolates obtained from symptomatic apple (<i>Malus domestica</i>) fruits in Türkiye were identified as <i>Aspergillus tubingensis</i> based on morphological characters and multilocus phylogenetic analyses of β-tubulin (<i>BenA</i>), calmodulin (<i>CaM</i>) and RNA polymerase II second largest subunit (<i>RPB2</i>) genes. Pathogenicity assays produced lesions on wounded pome fruits, whereas non-wounded fruits remained symptomless, indicating a wound-dependent infection pattern under the tested conditions. Cross-host inoculation assays on pear (<i>Pyrus communis</i>) and quince (<i>Cydonia oblonga</i>) revealed significant variation in susceptibility among cultivars, with ‘Deveci’ pear exhibiting the highest disease severity. For the two isolates examined, temperature assays indicated optimal mycelial growth at 31.0°C–32.7°C, while conidial germination peaked at approximately 35.4°C and remained high at 40°C, suggesting isolate-level differences between mycelial growth and conidial germination responses to elevated temperature. To our knowledge, this is the first multilocus-supported record of <i>A. tubingensis</i> causing postharvest black mould rot of apple in Türkiye, highlighting the importance of multilocus identification for resolving black aspergilli associated with pome fruit decay.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148462752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}