Journal of Phytopathology最新文献

Fusarium nirenbergiae Causes Fusarium Wilt of Common Bean (Phaseolus vulgaris) in Türkiye 镰刀菌引起的马铃薯枯萎病（Phaseolus vulgaris）

IF 1.1 4区农林科学

Journal of Phytopathology Pub Date : 2026-04-11 DOI: 10.1111/jph.70303

Müzeyyen Müge Kızılay, Göksel Özer

{"title":"Fusarium nirenbergiae Causes Fusarium Wilt of Common Bean (Phaseolus vulgaris) in Türkiye","authors":"Müzeyyen Müge Kızılay, Göksel Özer","doi":"10.1111/jph.70303","DOIUrl":"10.1111/jph.70303","url":null,"abstract":"<div>\u0000 \u0000 The Fusarium oxysporum species complex (FOSC) comprises cryptic taxa that cannot be reliably distinguished morphologically, necessitating multilocus phylogenetic approaches. During field surveys conducted in June–August 2022 in Central Anatolia (Konya, Nevşehir, and Niğde provinces), wilt symptoms were observed in commercial dry bean fields, with disease incidence ranging from 2% to 14%. Symptomatic plants exhibited chlorosis, stunting, and vascular browning of crown tissues. Fusarium-like isolates were consistently recovered and characterized morphologically. Two representative pathogenic isolates (NKZ and NKK3) that fulfilled Koch's postulates were selected for multilocus sequencing (tef1, cmdA and rpb2), revealing ≥ 99% identity to authenticated Fusarium nirenbergiae reference strains. Concatenated phylogenetic analysis placed the isolates in a well-supported clade with the ex-type strain CBS 840.88ᵀ, clearly separated from F. oxysporum sensu stricto and other FOSC taxa. This study provides the first multilocus phylogenetically confirmed report of Fusarium nirenbergiae causing Fusarium wilt on common bean in Türkiye and underscores the necessity of moving beyond the classical forma specialis concept in regional disease surveillance.\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 2","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147683512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Report of Neocosmospora keratoplastica Causing Root Rot in Adenium obesum in Brazil 新宇宙孢子虫引起巴西肥胖腺瘤根腐病的首次报道

IF 1.1 4区农林科学

Journal of Phytopathology Pub Date : 2026-04-05 DOI: 10.1111/jph.70253

Vitória B. Silva, Luís O. Viteri, Marcos P. S. Câmara, Ana Gabriele G. Amaral, André N. Oliveira, Paulo R. S. Fernandes, Dalmarcia Souza C. Mourão, Adalberto C. Café-Filho, Gil R. Santos

引用次数: 0

First Report of ‘Candidatus Phytoplasma trifolii’-Related Strain (16SrVI-D Subgroup) Associated With Yellows Disease on Crape Jasmine (Tabernaemontana divaricate) From India 印度产茉莉黄病相关菌株（16SrVI-D亚群）首次报道

IF 1.1 4区农林科学

Journal of Phytopathology Pub Date : 2026-04-05 DOI: 10.1111/jph.70297

Shobhit Maheshwari, Ram Prasad Kushvaha, Akshita Soni, Sunil Kumar Snehi

{"title":"First Report of ‘Candidatus Phytoplasma trifolii’-Related Strain (16SrVI-D Subgroup) Associated With Yellows Disease on Crape Jasmine (Tabernaemontana divaricate) From India","authors":"Shobhit Maheshwari, Ram Prasad Kushvaha, Akshita Soni, Sunil Kumar Snehi","doi":"10.1111/jph.70297","DOIUrl":"10.1111/jph.70297","url":null,"abstract":"<div>\u0000 \u0000 Crape jasmine is a beautiful evergreen flowering shrub and is native to India. During a survey in 2025, severe yellowing symptoms with stunted growth of the plant was observed on the crap jasmine plant at Bhopal, Central India. For the identification of ‘Candidatus phytoplasma species’ on crap jasmine, symptomatic and asymptomatic leaf samples were collected and total DNA was extracted. The phytoplasma 16S rRNA gene was successfully amplified by direct and nested PCR using isolated DNA and phytoplasma-specific primers from three symptomatic plants. The amplified ~1.2 kb nested PCR products from three samples were sequenced, and the resulting 16S rRNA gene nucleotide sequences were submitted to GenBank under the accession numbers PX442245, PX890108 and PX890109. Sequence analysis of all three isolates (PX442245, PX890108 and PX890109) revealed the highest nucleotide sequence identity (99%) and close phylogenetic relationships with reference strains of brinjal little leaf phytoplasma, ‘Candidatus Phytoplasma trifolii’ (16SrVI group), predominantly belonging to the 16SrVI-D subgroup. In silico RFLP analysis of the 16S rRNA gene sequences of the phytoplasma isolates under study showed the highest similarity to the reference strain of the 16SrVI-D subgroup, with similarity coefficients ranging from 0.93 to 0.86. To the best of our knowledge, this is the first report of a ‘Candidatus Phytoplasma trifolii’-related strain (16SrVI-D subgroup) associated with yellows disease of crape jasmine (Tabernaemontana divaricata) in India.\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 2","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147683202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergence of Southern Rice Black Streak Dwarf Virus in Sugarcane and Jungle Rice Reveals New Alternate Hosts 南方水稻黑条矮病毒在甘蔗和丛林水稻上的出现揭示了新的替代宿主

IF 1.1 4区农林科学

Journal of Phytopathology Pub Date : 2026-04-03 DOI: 10.1111/jph.70298

Gourav Vats, Abhishek Kumar, Pooja Sharma, Arushi Padiyal, Yashika Walia, Sunny Dhir

引用次数: 0

First Report of Pleurostoma richardsiae Causing Canker and Dieback of Avocado Trees in Mexico 墨西哥牛油果溃疡病和枯死病的牛油果胸膜瘤首次报道

IF 1.1 4区农林科学

Journal of Phytopathology Pub Date : 2026-04-01 DOI: 10.1111/jph.70295

Karen Guadalupe Cantú-Treviño, Adriana Gutiérrez-Díez, Salvador Ochoa-Ascencio

引用次数: 0

Identification of Novel Sources of Ascochyta Blight Resistance in Wild Relatives of Chickpea 鹰嘴豆野生近缘种抗疫病新来源的鉴定

IF 1.1 4区农林科学

Journal of Phytopathology Pub Date : 2026-03-30 DOI: 10.1111/jph.70292

Kanwardeep S. Rawale, Kurtis Schroeder, Kevin McPhee, Yung C. Chen, Weidong Chen, Kulvinder S. Gill

{"title":"Identification of Novel Sources of Ascochyta Blight Resistance in Wild Relatives of Chickpea","authors":"Kanwardeep S. Rawale, Kurtis Schroeder, Kevin McPhee, Yung C. Chen, Weidong Chen, Kulvinder S. Gill","doi":"10.1111/jph.70292","DOIUrl":"10.1111/jph.70292","url":null,"abstract":"<div>\u0000 \u0000 Ascochyta blight, caused by Ascochyta rabiei, is the most devastating disease affecting global chickpea production and is currently managed with partially resistant varieties and multiple fungicide applications in years of the epidemic. However, the identification of new pathotypes that are resistant to commonly used fungicides and increased aggressiveness against the current germplasm puts the sustainability of chickpea production under constant threat. Thus, identifying novel sources of genetic resistance against highly aggressive Ascochyta rabiei pathotypes followed by transfer to chickpea varieties is the first step in effective disease management. In this study, 90 chickpea accessions representing eight Cicer species were evaluated for ascochyta blight resistance using a reliable controlled conditions screening method against an A. rabiei pathotype prevalent in the Pacific Northwest, USA. The mean disease score across accessions ranged from 1.25 to 9 on a 1–9 rating scale, with 1 being the most resistant. Out of the eight Cicer species, the highest level of disease resistance was identified in C. bijugum and C. echinospermum germplasm, with the lowest disease scores of 1.25 and 2.82, respectively. Additional germplasm characterization based on the mean disease and disease spread across biological replicates revealed six novel accessions with a high level of genetic resistance, with a mean disease score between 1.25 and 1.8.\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 2","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147684267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ammi majus as a New Natural Host of Bean Common Mosaic Virus 蚕豆花叶病毒新天然寄主的研究

IF 1.1 4区农林科学

Journal of Phytopathology Pub Date : 2026-03-29 DOI: 10.1111/jph.70294

Vinicius Henrique Bello, Harri José Lorenzi, Jorge Alberto Marques Rezende, Elliot Watanabe Kitajima

{"title":"Ammi majus as a New Natural Host of Bean Common Mosaic Virus","authors":"Vinicius Henrique Bello, Harri José Lorenzi, Jorge Alberto Marques Rezende, Elliot Watanabe Kitajima","doi":"10.1111/jph.70294","DOIUrl":"10.1111/jph.70294","url":null,"abstract":"<div>\u0000 \u0000 In 2023, two Ammi majus plants showing mosaic symptoms were observed in the state of São Paulo, Brazil. Transmission electron microscope analysis of leaf extracts revealed the consistent presence of elongated, flexuous filamentous particles measuring 13–15 nm in width and 700–750 nm in length. Furthermore, analysis of ultrathin sections showed cytoplasmic cylindrical inclusions, characteristic of viruses of the genus Potyvirus. Molecular assays using universal primers, BCMV-specific primers, and amplicon sequencing confirmed the presence of bean common mosaic virus (BCMV, Potyvirus phaseovulgaris). Phylogenetic analysis grouped the BCMV isolate from A. majus (BCMV-Am) in a clade with BCMV isolates from Aristolochia spp. plants. Mechanical inoculation assays resulted in chlorotic local lesions in Chenopodium amaranticolor and C. quinoa, and systemic mosaic in Canavalia ensiformis and Phaseolus vulgaris cv. Jalo. However, the BCMV-Am isolate was not mechanically transmitted to Apium graveolens and Petroselinum crispum plants. To our knowledge, this is the first report of BCMV naturally infecting A. majus plants.\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 2","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147684248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Managing Brown Rot Disease of Citrus Fruit Using Plant Extracts 利用植物提取物防治柑橘褐腐病

IF 1.1 4区农林科学

Journal of Phytopathology Pub Date : 2026-03-26 DOI: 10.1111/jph.70254

Gbenga Oluwaseyi Alabi, Vinesh Maharaj, Jacquie van der Waals, Luke Invernizzi, Teresa Coutinho

{"title":"Managing Brown Rot Disease of Citrus Fruit Using Plant Extracts","authors":"Gbenga Oluwaseyi Alabi, Vinesh Maharaj, Jacquie van der Waals, Luke Invernizzi, Teresa Coutinho","doi":"10.1111/jph.70254","DOIUrl":"10.1111/jph.70254","url":null,"abstract":"Brown rot, caused by Phytophthora nicotianae and P. citrophthora, is a major disease of citrus fruit, leading to significant economic losses globally. Conventional fungicides are commonly used to manage this disease, but concerns have been raised due to environmental persistence, human toxicity and the emergence of resistant strains. This study evaluated the spore germination and mycelial growth inhibition of both Phytophthora species by extracts of 31 plant species in vitro and in vivo. DCM/MeOH extracts were tested against both pathogens using a microtiter plate assay and an amended medium assay. All bioactive extracts were further fractionated into seven fractions using solid phase extraction (SPE), which were subsequently tested against both pathogens. Phytochemical profiling was determined by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC–QTOF-MS) analysis. Extracts of seven plants (Artemisia afra, Dombeya rotundifolia, Eucomis regia, Olea europaea, Cannabis sativa, Peltophorum africanum and Mentha longifolia) inhibited both P. nicotianae and P. citrophthora with a MIC < 1 mg/mL and MFC < 2.5 mg/mL as compared to azoxystrobin (6.0 mg/mL). In the lemon fruit in vivo assays, A. afra reduced brown rot severity by > 50% compared to untreated controls, with superior performance to azoxystrobin, the standard fungicide. UPLC–QTOF-MS profiling of bio-active fractions identified scopoletin (A. afra), oleuropeinic acid (O. europaea) and bergenin (P. africanum) as putative contributors to the observed activity. These findings highlight A. afra as a promising natural product and suggest that other extracts warrant further evaluation for use as alternative oomyceticides in citrus post-harvest protection.","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 2","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jph.70254","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147585050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol Potential of Talaromyces spp. Against Root-Knot Nematodes With Main Focus on T. pinophilus Talaromyces spsp对根结线虫的生物防治潜力——以嗜松线虫为主

IF 1.1 4区农林科学

Journal of Phytopathology Pub Date : 2026-03-26 DOI: 10.1111/jph.70290

Rami Kassam, Madhurima Chatterjee, K. V. V. S. Kranti, Haritha Bollinedi, Deeba Kamil, Abolfazl Hajihassani, Victor Phani, Uma Rao

{"title":"Biocontrol Potential of Talaromyces spp. Against Root-Knot Nematodes With Main Focus on T. pinophilus","authors":"Rami Kassam, Madhurima Chatterjee, K. V. V. S. Kranti, Haritha Bollinedi, Deeba Kamil, Abolfazl Hajihassani, Victor Phani, Uma Rao","doi":"10.1111/jph.70290","DOIUrl":"10.1111/jph.70290","url":null,"abstract":"<div>\u0000 \u0000 Root-knot nematodes (Meloidogyne spp.) pose a significant threat to global agriculture causing substantial yield losses. Talaromyces is a well-known biocontrol fungus used against the root-knot nematodes. To assess the diversity of nematophagous Talaromyces spp. from the rhizospheric soils, we employed a novel technique that used target root-knot nematode as prey to isolate the fungi. A total of 26 Talaromyces isolates belonging to 12 nominal species were identified across varied agroclimatic zones of India. Detailed morphological and molecular characterisation revealed the presence of T. adpressus, T. oumae-annae and T. veerkampii, hitherto unreported from India. Nine Talaromyces spp. found in the present investigation were not known to possess nematophagous ability to date. Nematophagous ability of the isolated fungal species was confirmed through in vitro studies using M. incognita and M. graminicola. The isolate F17 (T. pinophilus) was found to induce 100% mortality of the nematodes. Further, in vivo studies conducted with F17 isolate using M. incognita-tomato and M. graminicola-rice pathosystems showed up to 79.8% and 74.7% reduction in nematode galling, respectively, along with plant growth promotion. Expression of green fluorescent protein in the F17 isolate helped track the progress and nature of juvenile parasitism. The GC–MS profiling of volatile organic compounds in F17 revealed the presence of 32 bioactive components that reportedly contain nematicidal potential, thus corroborating the in vitro mortality. The performance of the fungal isolate F17 (T. pinophilus) inculcates high confidence to propose it as a prospective bioagent to be commercially developed for root-knot nematode management.\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 2","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147579738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification, Pathogenicity, and Fungicide Sensitivity of Sclerotinia sclerotiorum Isolates Causing White Mould on Cucumber in Sinaloa, Mexico 墨西哥锡那罗亚州黄瓜白霉菌核菌分离株的鉴定、致病性及杀菌剂敏感性

IF 1.1 4区农林科学

Journal of Phytopathology Pub Date : 2026-03-24 DOI: 10.1111/jph.70287

María B. Contreras-Soto, Guillermo Márquez-Licona, Alma R. Solano-Báez, José A. Carrillo-Fasio, Isabel Cruz-Lachica, Isidro Márquez-Zequera, Raymundo S. García-Estrada, Juan M. Tovar-Pedraza

{"title":"Identification, Pathogenicity, and Fungicide Sensitivity of Sclerotinia sclerotiorum Isolates Causing White Mould on Cucumber in Sinaloa, Mexico","authors":"María B. Contreras-Soto, Guillermo Márquez-Licona, Alma R. Solano-Báez, José A. Carrillo-Fasio, Isabel Cruz-Lachica, Isidro Márquez-Zequera, Raymundo S. García-Estrada, Juan M. Tovar-Pedraza","doi":"10.1111/jph.70287","DOIUrl":"10.1111/jph.70287","url":null,"abstract":"<div>\u0000 \u0000 In January 2025, severe white mould symptoms were observed on cucumber fruits (Cucumis sativus L.) from plants grown under shade netting in Navolato, Sinaloa, Mexico. Morphological characterisation enabled the identification of Sclerotinia sclerotiorum, and its identity was confirmed through phylogenetic analysis using ITS region sequences. Isolates of S. sclerotiorum were inoculated onto cucumber fruits (European and pickling types), producing typical symptoms of fruit rot and white mould. In the in vitro sensitivity assay, five S. sclerotiorum isolates were evaluated and exhibited EC50 value ranges of 0.0098–0.0204, 0.0068–0.0570, 0.0006–0.0031, and 0.0968–3.1648 μg mL−1 for the fungicides pydiflumetofen, pyraclostrobin, fluazinam, and thiophanate-methyl, respectively. This study provides relevant information for the diagnosis and management of white mould in cucumber crops.\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"174 2","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147579857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0