The Effect of Bacillus subtilis and Bacillus velezensis on the Antioxidative Responses of Tomato Plants Facing Pseudomonas syringae pv. tomato DC3000 Infection

IF 1.1 4区农林科学 Q3 PLANT SCIENCES

Journal of Phytopathology Pub Date : 2025-10-02 DOI:10.1111/jph.70178

Sumaira, Elizaveta A. Gavrilova, Shamil Z. Validov, Humaira Yasmin, Airat R. Kayumov

{"title":"The Effect of Bacillus subtilis and Bacillus velezensis on the Antioxidative Responses of Tomato Plants Facing Pseudomonas syringae pv. tomato DC3000 Infection","authors":"Sumaira, Elizaveta A. Gavrilova, Shamil Z. Validov, Humaira Yasmin, Airat R. Kayumov","doi":"10.1111/jph.70178","DOIUrl":null,"url":null,"abstract":"<div>\n \n Tomato (Solanum lycopersicum), a globally significant crop, is highly susceptible to bacterial speck disease caused by Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). In this paper, we studied the effect of plant growth-promoting bacteria (PGPB) Bacillus subtilis MGMM36 and Bacillus velezensis MGMM30 on tomato resistance to Pst DC3000 under greenhouse conditions. Seed pre-treatment with either B. subtilis or B. velezensis improved plant viability (60% and 50% survival, respectively, compared to 25% in plants infected with Pst DC3000 alone) and mitigated pathogen-induced damage. B. subtilis provided stronger protection compared to B. velezensis: plants treated with B. subtilis showed a 20% reduction in shoot length (SL), a 10% reduction in root length (RL) and a 15% reduction in leaf area (LA) compared to the control. However, plants treated with B. velezensis exhibited a 30% decrease in SL, a 25% decrease in RL and a 22% decrease in LA. Furthermore, in infected plants grown from seeds previously treated with PGPB, no increase in CAT (SICAT), SOD (SISOD) and PTO gene expression was observed, in contrast to untreated plants. Both B. subtilis and B. velezensis increased the expression of the PR1 gene, restoring it to levels found in uninfected control plants. These findings underscore the potential of two strains of Bacillus spp. as sustainable biological agents to enhance disease resistance and promote tomato growth, with the aim of practical application for pesticide reduction and environmental protection.\n </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 5","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jph.70178","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Tomato (Solanum lycopersicum), a globally significant crop, is highly susceptible to bacterial speck disease caused by Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). In this paper, we studied the effect of plant growth-promoting bacteria (PGPB) Bacillus subtilis MGMM36 and Bacillus velezensis MGMM30 on tomato resistance to Pst DC3000 under greenhouse conditions. Seed pre-treatment with either B. subtilis or B. velezensis improved plant viability (60% and 50% survival, respectively, compared to 25% in plants infected with Pst DC3000 alone) and mitigated pathogen-induced damage. B. subtilis provided stronger protection compared to B. velezensis: plants treated with B. subtilis showed a 20% reduction in shoot length (SL), a 10% reduction in root length (RL) and a 15% reduction in leaf area (LA) compared to the control. However, plants treated with B. velezensis exhibited a 30% decrease in SL, a 25% decrease in RL and a 22% decrease in LA. Furthermore, in infected plants grown from seeds previously treated with PGPB, no increase in CAT (SICAT), SOD (SISOD) and PTO gene expression was observed, in contrast to untreated plants. Both B. subtilis and B. velezensis increased the expression of the PR1 gene, restoring it to levels found in uninfected control plants. These findings underscore the potential of two strains of Bacillus spp. as sustainable biological agents to enhance disease resistance and promote tomato growth, with the aim of practical application for pesticide reduction and environmental protection.

Abstract Image

查看原文本刊更多论文

枯草芽孢杆菌和velezensis芽孢杆菌对番茄植株抗氧化反应的影响番茄DC3000感染

番茄（Solanum lycopersicum）是一种全球重要作物，极易感染由丁香假单胞菌引起的细菌性斑点病。番茄DC3000 （Pst DC3000）。在温室条件下，研究了植物生长促进菌枯草芽孢杆菌MGMM36和velezensis芽孢杆菌MGMM30对番茄抗Pst DC3000的影响。用枯草芽孢杆菌或velezensis对种子进行预处理可以提高植物的生存能力（分别为60%和50%，而单独感染Pst DC3000的植物存活率为25%），并减轻病原体引起的损害。枯草芽孢杆菌提供了较强的保护：与对照相比，枯草芽孢杆菌处理的植株的茎长（SL）减少20%，根长（RL）减少10%，叶面积（LA）减少15%。然而,植物处理b velezensis展出SL下降了30%,下降了25% RL和LA下降了22%。此外，与未经处理的植株相比，经PGPB处理过的种子生长的受感染植株的CAT （SICAT）、SOD （SISOD）和PTO基因表达没有增加。枯草芽孢杆菌和白僵杆菌都增加了PR1基因的表达，使其恢复到未感染对照植物的水平。研究结果表明，这两株芽孢杆菌具有增强番茄抗病性和促进番茄生长的可持续生物制剂的潜力，有望在农药减量和环境保护方面得到实际应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.