Journal of Phytopathology最新文献

{"title":"Genome Characterisation and Pathogenicity Analysis of Iranian Isolates of Okra Enation Leaf Curl Virus (Begomovirus abelsmoschusenation) and Their Associated Satellites","authors":"Shima Heydari,&nbsp;Asra Salari,&nbsp;Jahangir Heydarnejad","doi":"10.1111/jph.70144","DOIUrl":"https://doi.org/10.1111/jph.70144","url":null,"abstract":"<div>\u0000 \u0000 <p>Begomoviruses and associated betasatellite molecules significantly impact crop yields in Middle Eastern countries. Previously, cotton leaf curl Gezira virus (CLCuGeV, <i>Begomovirus gossypigeziraense</i>) and the associated betasatellite, okra leaf curl Oman betasatellite (OLCuOMB, <i>Betasatellite abelmoschusomanense</i>), were detected in okra farms in Jiroft, south-eastern Iran. The current study describes the interaction between OLCuOMB and okra leaf curl enation virus (OELCuV, <i>Begomovirus abelsmoschusenation</i>) in okra farms in the same region. Both OELCuV and OLCuOMB, along with the <i>Gossypium darwinii</i> symptomless alphasatellite (GDarSLA, <i>Colecusatellite gossypiumdarwinii</i>), were detected in okra plants, and a similar viral association with only GDarSLA was found in diseased basil plants. The full-length genomes of OELCuV isolates from okra and basil shared 99.4% nucleotide identity with each other, and &gt; 97% with other Iranian isolates from papaya available in GenBank. To satisfy Koch's postulates, an infectious clone of OELCuV was constructed, and its pathogenicity was assessed with or without the OLCuOMB clone. Accordingly, agroinoculation with a combination of OELCuV and OLCuOMB resulted in more severe symptoms in okra plants compared to the milder symptoms produced following agroinoculation with the virus alone. The presence of alphasatellite and betasatellite molecules in association with various begomoviruses, and a turncurtovirus across different crops in south-eastern Iran indicates that geminivirus-satellite disease complexes are causing significant yield losses in this region.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Multi-Location and Multi-Year Field Trials Revealed Broad-Spectrum Resistance of Sorghum (Sorghum bicolor (L.) Moench) to Anthracnose (Colletotrichum sublineola)”","authors":"","doi":"10.1111/jph.70136","DOIUrl":"https://doi.org/10.1111/jph.70136","url":null,"abstract":"<p>Mekonen M., A. Chala, K. Tesfaye, T. Mengiste, H. Nida, T. Mekonnen, A. Tirfessa, G. Girma, Z. Mehary, H. Alemu, B. Kuru, M. Geleta. 2025. “Multi-Location and Multi-Year Field Trials Revealed Broad-Spectrum Resistance of Sorghum (<i>Sorghum bicolor</i> (L.) Moench) to Anthracnose (<i>Colletotrichum sublineola</i>).” <i>Journal of Phytopathology</i> 173: e70065. https://doi.org/10.1111/jph.70065</p><p>In the above article, the authors wish to correct the Funding Section as follows:</p><p>The authors declare financial support for this research from the Ethiopian Institute of Agricultural Research (EIAR) and the Department of Plant Breeding at the Swedish University of Agricultural Sciences (SLU). The contents are the sole responsibility of the authors and do not reflect the views of the funder.</p><p>We apologize for this error.</p>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jph.70136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to ‘Cultivar Resistance and Alternative Crops to Manage Fusarium Wilt in Lettuce Caused by Fusarium oxysporum f. sp. Lactucae Race 4 and the Emerging Race 4+’","authors":"","doi":"10.1111/jph.70135","DOIUrl":"https://doi.org/10.1111/jph.70135","url":null,"abstract":"<p>Mestdagh, H., T. Dockx, I. Vandevelde, J. Van Mullem, A. Decombel, K. Heungens, M. Höfte. 2025. “Cultivar Resistance and Alternative Crops to Manage Fusarium Wilt in Lettuce Caused by <i>Fusarium oxysporum</i> f. sp. <i>Lactucae</i> Race 4 and the Emerging Race 4+.” <i>Journal of Phytopathology</i> 173: e70072. https://doi.org/10.1111/jph.70072</p><p>In Table 5 of the above article, columns ‘<i>PSE1’</i> and ‘<i>PSL1’</i> should be reversed.</p><p>We apologize for this error.</p>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jph.70135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Colletotrichum godetiae Causing Leaf Anthracnose on Blackberry in Mexico","authors":"Joanna Nava-Delgado,&nbsp;Alma Rosa Solano-Báez,&nbsp;Miroslav Kolařík,&nbsp;Santos Gerardo Leyva-Mir,&nbsp;Hugo Beltran-Peña,&nbsp;Guillermo Márquez-Licona","doi":"10.1111/jph.70151","DOIUrl":"https://doi.org/10.1111/jph.70151","url":null,"abstract":"<div>\u0000 \u0000 <p>Blackberry is one of the four most important commercial berries, along with raspberry, strawberry, and blueberry. Mexico is the world's leading producer of blackberries, accounting for 21.8% of the global total. In October 2023, severe symptoms of leaf anthracnose were observed in a commercial blackberry plantation in Zacatlán, Puebla, Mexico. Based on morphological features, the fungi isolated from the diseased plants were identified as members of the <i>Colletotrichum acutatum</i> species complex. The multilocus phylogeny (ITS, <i>act</i>, <i>gapdh, his3, chs-1,</i> and <i>tub2</i>) confidently classified the isolates as <i>Colletotrichum godetiae</i>. Artificial inoculation of healthy leaves led to symptom development after 7 days, thereby fulfilling Koch's postulates. This is the first global report of <i>C. godetiae</i> causing leaf anthracnose on blackberry (<i>Rubus</i> spp. var. Tupy). Leaf anthracnose in blackberry plantations in Puebla, Mexico, requires further studies to analyse the pathogen's diversity, its geographic distribution, and alternative hosts, all aimed at guiding surveillance efforts and developing management strategies.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Juglans regia: A Novel Natural Host for Hostuviroid impedihumuli and Its Molecular Profiling","authors":"Feyzullah Yilmaz,&nbsp;H. Diğdem Oksal,&nbsp;H. Murat Sipahioğlu","doi":"10.1111/jph.70148","DOIUrl":"https://doi.org/10.1111/jph.70148","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Hostuviroid impedihumuli</i> is a circular, positive-sense, single-stranded RNA viroid, 295–303 nucleotides in length, belonging to the family Pospiviroidae and the genus <i>Hostuviroid</i>. The most stable secondary structure of viroid RNA has been shown to adopt a rod-like or semi-rod-like conformation. A comprehensive study was conducted to detect viroids in walnut (<i>Juglans regia</i>) trees. A total of 230 walnut samples were collected from various provinces across Türkiye and subjected to molecular analysis. Both one-step real-time RT-PCR and conventional RT-PCR methods were employed, using primers specifically designed for the detection of <i>H. impedihumuli</i>. Molecular analyses revealed that 11.3% of the samples were infected with <i>H. impedihumuli</i>. The viroid sequences obtained were compared with homologous sequences in the GenBank database, showing nucleotide similarity rates ranging from 89.5% to 99.3%. To the best of our knowledge, this study presents the first documented occurrence of <i>H. impedihumuli</i> in walnut, representing a novel host record for both Türkiye and the global plant health community.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Golovinomyces sordidus Causing Powdery Mildew of Plantago asiatica in India","authors":"Sabyasachi Banerjee,&nbsp;Shweta Bhimta,&nbsp;Himanshi Panchal,&nbsp;Shailesh Pandey","doi":"10.1111/jph.70145","DOIUrl":"https://doi.org/10.1111/jph.70145","url":null,"abstract":"<div>\u0000 \u0000 <p>Since April 2020, a powdery mildew disease has been consistently observed on <i>Plantago asiatica</i> in northern India. Microscopic examination of the associated fungus revealed morphological characteristics typical of the genus <i>Golovinomyces</i>. Molecular identification based on a BLASTn similarity search of the internal transcribed spacer region, along with phylogenetic analyses using maximum likelihood, maximum parsimony, and Bayesian inference methods, confirmed the species as <i>Golovinomyces sordidus</i>. Pathogenicity was verified through inoculation of healthy <i>P. asiatica</i> plants. To the best of our knowledge, this is the first confirmed report of <i>G. sordidus</i> causing powdery mildew on <i>P. asiatica</i> in India.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Meloidogyne enterolobii Infecting Dragon Fruit (Selenicereus undatus) in México","authors":"Alejandro Salinas-Castro,&nbsp;Laura Navarro-de la Fuente,&nbsp;Dinorah Lima-Rivera,&nbsp;María del Carmen Núñez-Camargo,&nbsp;Daniel López-Lima","doi":"10.1111/jph.70143","DOIUrl":"https://doi.org/10.1111/jph.70143","url":null,"abstract":"<div>\u0000 \u0000 <p>Dragon fruit is an economically important crop in Mexico. In summer 2023, severe galling symptoms were observed in a plantation in the state of Puebla. This study aimed to identify the species of root-knot nematode responsible for this damage. Red dragon fruit plants, which showed symptoms of yellowing, stunted growth and severe root galling, were collected. A single egg mass extracted from the roots was used to inoculate Rio Grande tomato plants to obtain a pure population. Morphological, morphometric and molecular (based on 28 s rDNA) analyses were used to identify the nematode species. Host suitability was confirmed by inoculation tests. Based on the results, we identified <i>Meloidogyne enterolobii</i> as the nematode linked to the serious symptoms seen in the field. To our knowledge, this is the first report of <i>M. enterolobii</i> infesting dragon fruit plants in Mexico, and based on our observations, this nematode could pose a significant threat to the crop.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Assisted Synthesis of AgNPs Using Artemisia lerchiana: Potential Antimicrobial Applications in Plant Disease Control","authors":"Gunay Hasanova,&nbsp;Sabina Omarova,&nbsp;Rovshan Khalilov,&nbsp;Afat Mammadova,&nbsp;Muhammad Zafar,&nbsp;Salman Majeed,&nbsp;Eman Alhomaidi,&nbsp;Mona Saleh Al Tami,&nbsp;Muhammad Rizwan Khan,&nbsp;Babar Ashfaq,&nbsp;Aleena Gul,&nbsp;Enkelejda Kucaj","doi":"10.1111/jph.70142","DOIUrl":"https://doi.org/10.1111/jph.70142","url":null,"abstract":"<div>\u0000 \u0000 <p>Silver nanoparticles are important because they have biological activity and physicochemical properties that make them useful. Silver, which is thought to be inert in nature, reacts with moisture in the skin and wound fluid to release ions, making it one of the most effective antimicrobial/antibacterial agents throughout history. However, this work illustrates the environmentally friendly production of silver nanoparticles (AgNPs) by employing leaf extract from <i>Artemisia lerchiana</i> as a stabilising and reducing agent. With a zeta potential of −20 mV and an average size of 165 nm, the biosynthesised AgNPs demonstrated stable colloidal characteristics. Gram-positive (<i>Staphylococcus aureus, Bacillus subtilis</i>), Gram-negative (<i>Escherichia coli, Pseudomonas aeruginosa</i>), and fungal (<i>Candida albicans</i>) strains were tested for antimicrobial efficacy. The results showed significant inhibitory effects, especially against <i>Bacillus subtilis</i> (MIC: 0.560 mg/mL) and <i>C. albicans</i> (MIC: 0.014 mg/mL). Additionally, using regression analysis, the ideal synthesis parameters were found to be 56°C, 6-unit extract, 2.4 mM AgNO₃, and an 8-min reaction time. These parameters produced AgNPs with improved antimicrobial activity (predicted MIC: 0.01 mg/mL). The results show that AgNPs mediated by <i>Artemisia lerchiana</i> have the potential to be an environmentally friendly antimicrobial agent, and they recommend more research into their mechanisms and potential uses in medicine.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol Potential of Acinetobacter lactucae and Pseudarthrobacter polychromogenes in Managing Pepper Bacterial Spot Disease","authors":"Deniz Caplik,&nbsp;Mustafa Küsek","doi":"10.1111/jph.70140","DOIUrl":"https://doi.org/10.1111/jph.70140","url":null,"abstract":"<div>\u0000 \u0000 <p>Bacterial spot disease, caused by <i>Xanthomonas euvesicatoria</i>, poses a significant threat to pepper (<i>Capsicum</i> spp.) production, necessitating sustainable control strategies. This study aimed to isolate and evaluate beneficial bacteria from healthy pepper plants as biocontrol agents and plant growth promoters. A total of 344 epiphytic and 192 endophytic bacterial isolates were collected from pepper fields in Şanlıurfa and Adıyaman, Türkiye, and screened for antagonistic activity against <i>X. euvesicatoria</i> and plant growth-promoting traits. Fourteen isolates were selected for greenhouse trials, applied via seed treatment, foliar spray, or combined methods. Notably, this study is the first to report <i>Acinetobacter lactucae</i> UEP50 and <i>Pseudarthrobacter polychromogenes</i> AEND14 as novel BCAs against <i>X. euvesicatoria</i> in peppers, highlighting their potential as effective microbial agents. <i>Acinetobacter lactucae</i> UEP50 and <i>Pseudarthrobacter polychromogenes</i> AEND14 showed promising biocontrol efficacy, reducing disease severity by up to 85% in controlled greenhouse trials with combined seed and foliar treatments. The combined application method demonstrated superior efficacy compared to single applications, achieving a 30%–40% higher reduction in disease severity than seed or foliar treatments alone, suggesting a synergistic effect that enhances disease suppression and plant growth promotion. Further field studies are needed to confirm these results. These isolates also demonstrated strong antagonistic activity (inhibition zones up to 27 mm) and plant growth-promoting traits, including indole-3-acetic acid production (up to 126.92 μg/mL) and phosphate solubilisation. This study reports <i>A. lactucae</i> and <i>P. polychromogenes</i> as promising biocontrol agents against <i>X. euvesicatoria</i> in peppers, suggesting their potential role in integrated pest management. Further literature review is needed to confirm the novelty of this finding. These findings suggest potential for developing microbial-based strategies to enhance pepper production sustainability. However, further research is necessary to validate field efficacy and optimise application methods.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and Biochemical Responses of Tomato Plants to White Mould Affected by Silver Nanoparticles","authors":"Beatriz Souza Damasceno,&nbsp;Larissa Cavalcante Almeida,&nbsp;Luciana Tajany Dias do Nascimento,&nbsp;Thaís Ribeiro Santiago,&nbsp;Edivan Rodrigues de Souza,&nbsp;Jonas Alberto Rios","doi":"10.1111/jph.70139","DOIUrl":"https://doi.org/10.1111/jph.70139","url":null,"abstract":"<p>Tomato (<i>Solanum lycopersicum</i> L.) is one of the most widely cultivated vegetables globally and its production is frequently affected by biotic factors, such as <i>Sclerotinia sclerotiorum</i>, the causal agent of white mould. This study aims to evaluate the direct effect of silver nanoparticles (AgNPs) on the in vitro growth of <i>S. sclerotiorum</i>, measured by mycelial growth velocity index (MGVI) and mycelial diameter (MD), in addition to the in vivo effects on tomato plants infected with <i>S. sclerotiorum</i> at epidemiological, physiological and biochemical scales. The results showed that both MGVI and MD decreased as AgNP concentrations increased, indicating a direct inhibitory effect of AgNPs on <i>S. sclerotiorum</i>. In the in vivo experiments, plants pretreated with AgNPs showed lower white mould severity, reflected in lower lesion size (LS) and area under the disease progress curve (AUDPC) values. These results were associated with preserved concentrations of photosynthetic pigments and the maximum quantum efficiency of photosystem II (<i>F</i>_v/<i>F</i>_m). The reduction in cellular damage, due to lower symptom severity, was correlated with decreased enzymatic activities of SOD, CAT, POX and APX. On the other hand, PAL activity was significantly higher in treated plants, suggesting an enhanced activation of defence pathways. In conclusion, the application of AgNPs preserved photosynthetic capacity by mitigating cellular infection and strengthened the defence response of tomato plants, showing its potential as an effective control strategy against <i>S. sclerotiorum</i>. However, additional studies are required to evaluate the performance of the AgNPs under field conditions, particularly in regions where the disease is endemic.</p>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jph.70139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}