{"title":"Disease Occurrence, Distribution and Intensity of Finger Millet Blast in Northwest Amhara, Ethiopia","authors":"Melkamu Andargie, Merkuz Abera, Tesfaye Alemu, Berhanu Bekele","doi":"10.1111/jph.70159","DOIUrl":"https://doi.org/10.1111/jph.70159","url":null,"abstract":"<div>\u0000 \u0000 <p>Blast disease significantly hampers finger millet production and causes considerable yield reductions. This research aimed to assess the prevalence and severity of finger millet blast in the major finger millet-growing districts of Northwestern Amhara. Field surveys were carried out in 270 farmer fields across five districts during the main growing seasons from September to November in 2022 and 2023. Blast disease was identified in all surveyed districts, with different incidence levels affected by agroecological conditions, farming methods, and crop varieties. Incidence rates varied from 15.21% to 58.26%, with Siemen Achefer showing the highest mean incidence of 56.54%, while Dangila had the lowest value of 17.69%. The overall average incidence across all districts was 33.54%. The severity of the disease also differed significantly, ranging from 18.24% to 86.98%. The highest severity has been recorded in Jabi Tehnan district, with an average severity of 65.38%. Conversely, the lowest mean severity was scored in Takusa, with a mean of 36.16% with the overall average severity of 51.50% across all districts. Variations in cultivars, crop management practices and agroecological factors may have influenced the intensity of blast disease. The findings highlight the significant threat that blast disease poses to finger millet cultivation in the studied areas. The rising incidence and severity of the disease indicate a growing risk of significant yield losses. Consequently, immediate measures are required to establish effective management strategies for blast control to improve finger millet production and productivity.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 5","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of Reliable Diagnostic Techniques for Banana Bract Mosaic Virus and Field Survey in Taiwan","authors":"Yu-Chun Lin, Meng-Ling Wu, Ting-Hsuan Hung","doi":"10.1111/jph.70157","DOIUrl":"https://doi.org/10.1111/jph.70157","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Potyvirus musae</i> (<i>Banana bract mosaic virus</i>, BBrMV), a member of the genus <i>Potyvirus</i>, can cause yield losses of up to 70% and is considered an important quarantine pathogen in international banana germplasm exchange. With the global movement of banana germplasm having increased in recent years, there is an urgent need for effective and scalable diagnostic tools for BBrMV detection in Taiwan. In this study, the recombinant BBrMV coat protein was expressed in <i>Escherichia coli</i> and used as an antigen to produce monoclonal antibodies. A specific monoclonal antibody, E9C11, was developed, and the optimal sampling strategy and extraction buffer were also established. The E9C11-based ELISA assay demonstrated sufficient sensitivity and specificity for large-scale indexing applications, including seedling certification and quarantine inspection. Furthermore, a field survey was conducted to understand the current infection status of BBrMV in Taiwan. The results indicated a low prevalence of BBrMV in commercial banana production areas, suggesting that the virus is not yet widespread but remains a potential threat. This study provides both a practical diagnostic tool and updated epidemiological data for effective BBrMV management in Taiwan.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 5","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. K. M. Sahfiqul Islam, Rakibul Hasan, Rumana Akter, Mohammad Ashik Iqbal Khan, Md. Atiqur Rahman Khokon, Mohammad Abdul Latif
{"title":"Influence of Temperature, Light and Surfactants on the Longevity of Silver Nanoparticles Synthesised From Ipomoea carnea for the Control of Rhizoctonia solani","authors":"A. K. M. Sahfiqul Islam, Rakibul Hasan, Rumana Akter, Mohammad Ashik Iqbal Khan, Md. Atiqur Rahman Khokon, Mohammad Abdul Latif","doi":"10.1111/jph.70146","DOIUrl":"https://doi.org/10.1111/jph.70146","url":null,"abstract":"<div>\u0000 \u0000 <p>This study explores the long-term effectiveness and stability of silver nanoparticles (AgNPs) synthesised using <i>Ipomoea carnea</i> extract against <i>Rhizoctonia solani</i>. AgNPs were stored under four conditions: room temperature (25°C) with light exposure, room temperature in darkness, 4°C in light and at 4°C in darkness; their antifungal activity was assessed over 360 days. AgNPs stored at 4°C in the dark showed sustained antifungal activity, while light exposure accelerated their degradation, reducing effectiveness by 120 days. UV–vis spectroscopy, particle size analysis and zeta potential confirmed increased nanoparticle oxidation due to light exposure. Under a higher concentration of NaCl (3 M), polyvinylpyrrolidone (PVP) and polyethylene glycol (PEG) were evaluated as stabilising agents. PVP exhibited the best protection against aggregation of AgNPs, followed by PEG. PVP offered superior protection against aggregation and maintained stability for up to 270 days, particularly at 4°C in darkness. Zeta potential analysis showed a moderate decline in nanoparticle stability over time, with PVP-stabilised AgNPs retaining a range of −28.7 to −22.1 mV. Antifungal assays demonstrated over 91.4% efficacy of PVP-stabilised AgNPs for 270 days, highlighting their potential as long-term antifungal agents. This study emphasises the importance of storage conditions and stabilisers in maintaining the efficacy of biosynthesised AgNPs.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arsalan Ahmad, Muhammad Tariq Manzoor, Muhammad Shafiq, MuhammadMudasar, Muskan Amjad, Sajid Ali, MubeenSarwar, Muhammad Shafiq Shahid
{"title":"A Novel Method to Classify and Study Chickpea Chlorotic Dwarf Virus (CpCDV) Strains Based on Evolutionary Diversity and Cis-Regulatory Elements (CRE)","authors":"Arsalan Ahmad, Muhammad Tariq Manzoor, Muhammad Shafiq, MuhammadMudasar, Muskan Amjad, Sajid Ali, MubeenSarwar, Muhammad Shafiq Shahid","doi":"10.1111/jph.70156","DOIUrl":"https://doi.org/10.1111/jph.70156","url":null,"abstract":"<div>\u0000 \u0000 <p>Chickpea chlorotic dwarf virus (CpCDV) is a primary virus belonging to the Mastrevirus genus that infects dicotyledonous crops like chickpeas and cotton. The current study investigates the evolutionary and regulatory activities of CpCDV strains utilising phylogenetic and recombination analysis. The objective of this research is to uncover a potential Cis-regulatory element (CRE) that will allow us to classify different strains based on the functions they govern in various plant parts. NCBI provided 268 full-length sequences of the CpCDV genome, and the Sequence Demarcation Tool generated the homology graph. Only 36 sequences were used, with homology ranging from 94% to 100%. Phylogenetic analysis utilising the neighbour-joining method revealed the various strains in clades and their surrounding strains. Recombination analysis identified strain CpCDV21 as the potential major parental strain involved in evolutionary and recombination events, along with 11 other recombinant variants. These analyses helped to predict the genomic modelling, divergence, and rate of exchange. Furthermore, CRE analysis identified the distinct strains that are linked to various categorical elements, such as hormone-responsive strains CpCDV17 and CpCDV20, which are dominantly present in the Sudan region and control major HREs, such as ABRE and TGACG. The stress-responsive elements, such as WUN motifs and MYB, are dominant in the promoter region of strain CpCDV13 that originated in Burkina Faso. Other stress-responsive elements, such as WRE3, STRE and light-responsive, including GT1 and G-box, while tissue-specific O2-site element present in major amounts. Such elements are crucial not only for plant growth and development but also for defence. CpCDV strains employ these elements to control the genomic machinery and therefore regulate plant function. These findings improve our knowledge of CpCDV's cis-regulatory components, recombination patterns, and genetic composition. In the future, this information can be used to develop resistance and improve crop quality through a CRE-mediated strategy.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zia Ur Rehman, Aamir Iqbal, Muhammad Imtiaz, Jabbar Khan, Muhammad Rameez Khan, Muhammad Awais, Jinbiao Ma, Sajid Ali
{"title":"Spatial and Temporal Variability in Wheat Yellow Rust Prevalence Across the Pak-Afghan Bordering Hindukush Region of Pakistan: A Four-Year Surveillance Study (2019–2022)","authors":"Zia Ur Rehman, Aamir Iqbal, Muhammad Imtiaz, Jabbar Khan, Muhammad Rameez Khan, Muhammad Awais, Jinbiao Ma, Sajid Ali","doi":"10.1111/jph.70154","DOIUrl":"https://doi.org/10.1111/jph.70154","url":null,"abstract":"<div>\u0000 \u0000 <p>The yellow rust caused by <i>Puccinia striiformis</i> has been shown to be very frequent and highly diverse in the Himalayan region of Pakistan, though little is known about the disease in the Pak-Afghan bordering Hindukush region of Pakistan. We assessed the spatial and temporal variability in the prevalence of wheat yellow rust across the Hindukush region of Pakistan through a comprehensive 4-year surveillance effort (2019–2022). Covering 1145 fields across multiple districts, the surveillance provided insights into yellow rust distribution in different regions: Peshawar Valley, Western Hindukush, Northern Hindukush and Southern Hindukush. Yellow rust emerged as the primary wheat disease in the studied areas, affecting 83% of surveyed fields, with 29% exhibiting a severity of ≥ 50%. The highest incidence occurred in Peshawar Valley (98%) followed by Northern Hindukush (83%), Western Hindukush (78%) and Southern Hindukush (78%). Severity was notably high in Peshawar Valley and Western Hindukush (33% each), whereas Southern Hindukush recorded the lowest (17%). Over the years, yellow rust manifested with high pressure in 2020 (97%) and 2019 (96%), transitioning to low pressure in 2021 (79%) and 2022 (60%). Among varieties scored, Galaxy, Pirsabak-2013, Pirsabak-2005 and Pakhtunkhwa-2015 exhibited high susceptibility (ACI > 40), whereas Fakhre-Bakkar-2017, Anaaj-2017, Barani-2017 and Akbar-2019 demonstrated resistant reactions (ACI < 10). This study's findings offer valuable insights for plant breeding programmes aimed at enhancing crop productivity in the Hindukush region of Pakistan while considering the relative regional risk of the disease.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chickpea Wilt Caused by Fusarium oxysporum in China","authors":"Wenjin Chen, Xiaoyong He, Ziqiang Zhang, Yingnan Mu, Yuchen Cheng, Chao Zhang, Yafang Fan, Yuanzheng Zhao","doi":"10.1111/jph.70153","DOIUrl":"https://doi.org/10.1111/jph.70153","url":null,"abstract":"<div>\u0000 \u0000 <p>Chickpea wilt is a severe disease in China, primarily observed from the seedling to pod-forming stages with characteristic symptoms including foliar chlorosis, vascular browning, and progressive plant wilting. In the present study, two fungal isolates YF1 and YF2 were isolated from symptomatic root tissues of diseased chickpea plants and identified as <i>Fusarium oxysporum</i> through comprehensive morphological examination and molecular analysis using ITS and EF-1α gene sequences. Pathogenicity was confirmed through controlled inoculation experiments on both seeds and seedlings, which reproduced the characteristic disease symptoms observed under field conditions. This is the first report of <i>F. oxysporum</i> as the causal agent of chickpea wilt disease in China, establishing a foundation for developing effective disease management strategies for this economically important crop.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luana B. Montelo, Marcos P. S. Câmara, Ana Gabriele G. Amaral, André N. Oliveira, Cristiano B. Moraes, Marcos V. Giongo, Marcio A. Ootani, Adalberto C. Café-Filho, Eugênio E. Oliveira, Gil R. Santos
{"title":"Colletotrichum siamense Identified as the Causal Agent of Anthracnose in Eugenia dysenterica in the Brazilian Cerrado","authors":"Luana B. Montelo, Marcos P. S. Câmara, Ana Gabriele G. Amaral, André N. Oliveira, Cristiano B. Moraes, Marcos V. Giongo, Marcio A. Ootani, Adalberto C. Café-Filho, Eugênio E. Oliveira, Gil R. Santos","doi":"10.1111/jph.70149","DOIUrl":"https://doi.org/10.1111/jph.70149","url":null,"abstract":"<p><i>Eugenia dysenterica</i>, commonly known as cagaiteira, is a plant native to the Cerrado biome that produces edible fruits and has medicinal properties. Cagaita fruit exhibiting anthracnose symptoms were collected from naturally occurring plants in native fragments of the Cerrado Biome at Gurupi, Tocantins, Brazil. Pathogenicity tests and microscopic examinations of infected tissues showed acervuli and conidia typical of <i>Colletotrichum</i> as the cause of the anthracnose symptoms. Molecular identification was performed by amplifying and sequencing the partial region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the intergenic spacer between DNA lyase and the mating-type locus MAT1-2-1 (APN2/MAT-IGS), glutamine synthetase (GS) and β-tubulin (TUB2). Maximum-likelihood multi-locus analysis placed the three isolates with strains of <i>C. siamense</i> with 99% bootstrap support. This study provides the first report of <i>C. siamense</i> causing anthracnose in <i>E. dysenterica</i> worldwide.</p>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jph.70149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan M. Tovar-Pedraza, Víctor H. Aguilar-Pérez, Karla Y. Leyva-Madrigal, Ana M. López-López, Guadalupe A. Mora-Romero, Santos G. Leyva-Mir, Elizabeth García-León
{"title":"A New Fungal Disease of Maize Caused by Clonostachys chloroleuca in Mexico","authors":"Juan M. Tovar-Pedraza, Víctor H. Aguilar-Pérez, Karla Y. Leyva-Madrigal, Ana M. López-López, Guadalupe A. Mora-Romero, Santos G. Leyva-Mir, Elizabeth García-León","doi":"10.1111/jph.70152","DOIUrl":"https://doi.org/10.1111/jph.70152","url":null,"abstract":"<div>\u0000 \u0000 <p>In March 2023, symptoms of root rot, growth reduction, yellowing, and wilting were observed in commercial maize fields in Angostura, Sinaloa, Mexico. Morphological and phylogenetic approaches confirmed the identity of <i>Clonostachys chloroleuca</i>. Pathogenicity tests were conducted, and Koch's postulates were fulfilled with three isolates of this fungus. This is the first report of <i>C. chloroleuca</i> causing maize wilt in Mexico and worldwide.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Bindu, H. D. Vinay Kumar, Shridhar Hiremath, Mantesh Muttappagol, M. Nandan, Devaraja, C. R. Jahir Basha, K. S. Shankarappa, V. Venkataravanappa, C. N. Lakshminarayana Reddy
{"title":"Complete Genome Analysis and Molecular Characterisation of Horsegram Yellow Mosaic Virus Infecting Pole Bean (Phaseolus vulgaris L.) in Karnataka","authors":"J. Bindu, H. D. Vinay Kumar, Shridhar Hiremath, Mantesh Muttappagol, M. Nandan, Devaraja, C. R. Jahir Basha, K. S. Shankarappa, V. Venkataravanappa, C. N. Lakshminarayana Reddy","doi":"10.1111/jph.70150","DOIUrl":"https://doi.org/10.1111/jph.70150","url":null,"abstract":"<div>\u0000 \u0000 <p>The yellow mosaic disease (YMD) caused by begomoviruses is a major constraint for the production of pole bean (<i>Phaseolus vulgaris</i> L.) in India. A survey was carried out in the eastern dry zone of Karnataka during 2019–20 to record the incidence of yellow mosaic disease in pole bean, which revealed the ubiquitous prevalence of YMD in pole bean ranging from 6.02% to 80.74%. Leaf samples collected (symptomatic and asymptomatic) were subjected to begomovirus detection using specific primers. Twelve samples, representing all the 12 taluks in the surveyed region, were considered for full genome amplification by RCA, cloned, and sequenced. The genome length of the 12 current isolates ranged from 2718 to 2744 and 2668–2671 nucleotides for DNA-A and DNA-B, respectively. Sequence analysis using the Sequence Demarcation Tool (SDT) showed > 91% nucleotide identity of current isolates (DNA-A) with other horsegram yellow mosaic virus (HgYMV) isolates available in GenBank. As per existing ICTV criteria, all the current isolates can be considered strains of HgYMV. Further, DNA-B associated with all the 12 isolates also shared > 91% nucleotide identity with DNA-B of HgYMV isolates, indicating the absence of component re-assortment in HgYMV. Variation in the pairwise nucleotide identity and phylogenetic analysis confirmed the existence of new strains within the current HgYMV isolates. GC plot analysis reveals potential recombination in the low GC-rich regions. Further, recombination breakpoint analysis indicated intra-species recombination in both DNA-A and DNA-B, which might have driven the origin of new strains in HgYMV. This is the first comprehensive study on begomovirus isolates associated with the yellow mosaic disease of pole bean based on complete genome sequencing in the world.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144853850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liting Luo, Ali Kamran, Keyi Hu, Nisar Uddin, Mehran Khan, Atul Kumar Srivastava, Yingjun Zhang, Sanwei Yang, Xin Xie
{"title":"High-Throughput Sequencing Analysis Reveals Tomato Yellow Leaf Curl Virus Infecting Passion Fruit (Passiflora edulis) Plants in China","authors":"Liting Luo, Ali Kamran, Keyi Hu, Nisar Uddin, Mehran Khan, Atul Kumar Srivastava, Yingjun Zhang, Sanwei Yang, Xin Xie","doi":"10.1111/jph.70147","DOIUrl":"https://doi.org/10.1111/jph.70147","url":null,"abstract":"<div>\u0000 \u0000 <p>Passion fruit (<i>Passiflora edulis</i> Sims) production in China has been significantly affected by plant viruses, with Tomato yellow leaf curl virus (TYLCV) being a significant concern. TYLCV mainly infects hosts from the Solanaceae, Cucurbitaceae and Poaceae families. In this study, we aimed to detect this virus in the Passifloraceae family, along with the East Asian passiflora virus (EAPV) and Telosma mosaic virus (TeMV), which have been reported in passion fruit. We analysed (August–November 2024) passion fruit plants with symptoms of severe chlorosis, interveinal yellowing and chlorotic blotches of older leaves in Rongjiang, Guizhou Province. Small RNA sequencing indicated the presence of the following five viruses in the tested leaf samples of passion fruit: EAPV, TeMV, TYLCV and Passion fruit severe mottle virus. TYLCV was the third major contributor after EAPV and TeMV. A full-length genome, consisting of 2781 bp, was constructed for isolate TYLCV-GZ. Polymerase chain reaction assay of randomly selected 48 leaf samples confirmed the presence of the newly characterised TYLCV-GZ isolate. A high nucleotide and amino acid similarity percentage (> 97%) was recorded using corresponding sequences in GenBank. Phylogenetic analysis revealed a close relationship between TYLCV-GZ and tomato-infecting isolates from China and Spain. These findings can help prevent the spread of this virus to other areas and improve passion fruit cultivation.</p>\u0000 </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}