Journal of Physiological Sciences最新文献_第3页

Distinct molecular profiles in human aortic smooth muscle cells under cyclic stretch and hydrostatic pressure. 在循环拉伸和静水压力下，人主动脉平滑肌细胞的不同分子谱。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-07-23 DOI: 10.1016/j.jphyss.2026.100090

Takahiro Kusume, Hana Inoue, Noriaki Arakawa, Kazuhiro Satomi, Utako Yokoyama

{"title":"Distinct molecular profiles in human aortic smooth muscle cells under cyclic stretch and hydrostatic pressure.","authors":"Takahiro Kusume, Hana Inoue, Noriaki Arakawa, Kazuhiro Satomi, Utako Yokoyama","doi":"10.1016/j.jphyss.2026.100090","DOIUrl":"10.1016/j.jphyss.2026.100090","url":null,"abstract":"Vascular smooth muscle cells are constantly subjected to cyclic stretch and hydrostatic pressure in vivo, yet the molecular responses to these stimuli remain incompletely understood. We performed transcriptome and secretome analyses to characterize the responses of human aortic smooth muscle cells (HASMCs) to cyclic stretch (10%; S10) or cyclic hydrostatic pressure corresponding to normotensive (120/60 mmHg; P120) or hypertensive (180/120 mmHg; P180) conditions. Principal component analysis and hierarchical clustering revealed distinct transcriptomic and secretomic profiles in P180 compared with S10 and P120. P180 also exhibited the largest number of differentially expressed genes, including several not previously linked to mechanical stress. Among these, vasoactive intestinal peptide receptor 1 (VIPR1), a regulator of vascular tone, was markedly upregulated. These distinct expression profiles induced by hypertensive hydrostatic pressure may provide insight into physiological and pathological responses in HASMCs.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 3","pages":"100090"},"PeriodicalIF":3.3,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13452211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148630194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid water transfer from extracellular to intracellular spaces following oral ingestion. 口服后水分从细胞外迅速转移到细胞内。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-07-10 DOI: 10.1016/j.jphyss.2026.100088

Hiroyuki Terawaki, Masahiko Y Kida

引用次数: 0

Effects of empagliflozin alone and in combination with exercise on the soleus muscle in obese type 2 diabetic rats. 恩格列净单用及联合运动对肥胖2型糖尿病大鼠比目鱼肌的影响。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-07-07 DOI: 10.1016/j.jphyss.2026.100087

Kazuho Inoue, Saori Sekiguchi, Yuji Ogura, Seiko Hoshino, Kimie Katayama, Junko Asano, Takayuki Akagi, Junko Igarashi-Migitaka, Shiika Watanabe, Yoshio Nagai, Kenjiro Kimura, Yugo Shibagaki, Atsuko Kamijo-Ikemori

{"title":"Effects of empagliflozin alone and in combination with exercise on the soleus muscle in obese type 2 diabetic rats.","authors":"Kazuho Inoue, Saori Sekiguchi, Yuji Ogura, Seiko Hoshino, Kimie Katayama, Junko Asano, Takayuki Akagi, Junko Igarashi-Migitaka, Shiika Watanabe, Yoshio Nagai, Kenjiro Kimura, Yugo Shibagaki, Atsuko Kamijo-Ikemori","doi":"10.1016/j.jphyss.2026.100087","DOIUrl":"10.1016/j.jphyss.2026.100087","url":null,"abstract":"This study assessed whether empagliflozin (EMPA) combined with endurance exercise further improves the soleus muscle in spontaneously diabetic Torii fatty rats, a model of obese type 2 diabetes. Male rats were divided into untreated control, exercise, EMPA, or EMPA + exercise groups. Age-matched Sprague-Dawley rats served as nondiabetic controls. Treadmill training and EMPA (0.03% in chow) were provided from 8 to 16 weeks of age. Both EMPA and the combined intervention improved blood glucose and insulin resistance. Additionally, the combined treatment reduced total cholesterol and triglycerides compared with EMPA alone. The cross-sectional area of type I fibers in the soleus tended to improve with all interventions. Combination treatment significantly enhanced citrate synthase activity, mitochondrial functional protein expression, and autophagic flux. These findings suggest that although EMPA does not adversely affect the soleus muscle, combining it with endurance exercise confers superior benefits via improved mitochondrial status, autophagy, and lipid metabolism.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 3","pages":"100087"},"PeriodicalIF":3.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13351120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148405357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Middle-aged mice show delayed and destabilized food-anticipatory circadian activity under restricted feeding. 在限制喂养下，中年小鼠表现出延迟和不稳定的食物预期昼夜节律活动。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-07-01 Epub Date: 2026-04-02 DOI: 10.1016/j.jphyss.2026.100071

Sachi N Ohno, Nana N Takasu, Hitoshi Uchida, Takahiro J Nakamura, Wataru Nakamura, Mitsutaka Sugimura

{"title":"Middle-aged mice show delayed and destabilized food-anticipatory circadian activity under restricted feeding.","authors":"Sachi N Ohno, Nana N Takasu, Hitoshi Uchida, Takahiro J Nakamura, Wataru Nakamura, Mitsutaka Sugimura","doi":"10.1016/j.jphyss.2026.100071","DOIUrl":"10.1016/j.jphyss.2026.100071","url":null,"abstract":"Mice are nocturnal and normally feed during the night; however, timed daily feeding can markedly alter behavioral and physiological rhythms. A well-known example is food-anticipatory activity (FAA), characterized by increased locomotor activity before feeding. In this study, we examined age-related effects on FAA under temporally restricted feeding (RF). Male C57BL/6 J mice aged 12 weeks and 54 weeks were maintained under a 12:12 light-dark cycle and subjected to a 4-h daily RF schedule. Wheel-running activity, feeding timing, intake, and body weight were monitored. Under ad libitum conditions, both age groups exhibited nocturnal activity and feeding. During RF, FAA emerged significantly earlier in young mice than in middle-aged mice. After returning to ad libitum feeding, daytime activity gradually merged into nocturnal rhythms, although some middle-aged mice showed unstable patterns. These results indicate that FAA reflects a circadian system distinct from the suprachiasmatic nucleus and that aging delays and destabilizes its expression.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 2","pages":"100071"},"PeriodicalIF":3.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147645796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward the promotion of One Health - Part II: Interdisciplinary research cooperation between digital transformation and exposome. 迈向同一个健康——第二部分：数字化转型与暴露的跨学科研究合作。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-07-01 Epub Date: 2026-04-26 DOI: 10.1016/j.jphyss.2026.100078

Takeshi Yoneshiro, Yoshito Kumagai, Keiko Nohara, Shingo Iwami, Naoki Honda, Nobuhiko Ohno, Motohiro Nishida

{"title":"Toward the promotion of One Health - Part II: Interdisciplinary research cooperation between digital transformation and exposome.","authors":"Takeshi Yoneshiro, Yoshito Kumagai, Keiko Nohara, Shingo Iwami, Naoki Honda, Nobuhiko Ohno, Motohiro Nishida","doi":"10.1016/j.jphyss.2026.100078","DOIUrl":"10.1016/j.jphyss.2026.100078","url":null,"abstract":"Human activities increasingly disrupt global ecosystems, contributing to climate change, biodiversity loss, and emerging health threats. In response, the One Health framework has gained attention as an integrative approach encompassing human, animal, and environmental health. In a symposium at APPW2025, experts in Exposome science and Digital Transformation discussed how interdisciplinary integration can advance predictive and preventive medicine. This review summarizes five key topics: Exposome as a determinant of disease risk, epigenetic mechanisms encoding environmental memory, environmental programming of brown adipose tissue, adaptive prioritization of environmental signals, and simulation-based drug repurposing. Collectively, these studies highlight a paradigm shift from conventional linear exposure-disease models toward a systems-level understanding integrating cumulative exposures, biological memory, and predictive modeling. The convergence of Exposome science and Digital Transformation provides a foundation for advancing One Health into a predictive and actionable scientific framework.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 2","pages":"100078"},"PeriodicalIF":3.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13141750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmaceutical agents targeting K_ATP channel modulate sweet taste sensitivity in mice. 靶向KATP通道的药物对小鼠甜味敏感性的调节作用。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-07-01 Epub Date: 2026-06-05 DOI: 10.1016/j.jphyss.2026.100082

Chika Sawai, Kuanyu Wang, Kengo Horie, Yoshihiro Mitoh, Hirotaka Ueda, Hiroshi Kamioka, Ryusuke Yoshida

{"title":"Pharmaceutical agents targeting KATP channel modulate sweet taste sensitivity in mice.","authors":"Chika Sawai, Kuanyu Wang, Kengo Horie, Yoshihiro Mitoh, Hirotaka Ueda, Hiroshi Kamioka, Ryusuke Yoshida","doi":"10.1016/j.jphyss.2026.100082","DOIUrl":"10.1016/j.jphyss.2026.100082","url":null,"abstract":"Sweet detection involves at least two mechanisms: a G-protein coupled sweet taste receptor (Tas1r2/Tas1r3) and glucose transporters. As in pancreatic β-cells, glucose transport may lead to closure of ATP-sensitive potassium (KATP) channels. Since expression of KATP channels in sweet taste cells has been reported, modulation of KATP channel activity would affect sweet taste sensitivity. Here, we examined the effect of glibenclamide (a KATP channel closer) and diazoxide (an opener) on mouse taste behavior. Glibenclamide selectively reduced taste sensitivity to glucose without affecting responses to sucrose or sucralose compared to insulin, suggesting selective impairment of the transporter-dependent pathway. In contrast, diazoxide broadly suppressed responses to all tested sweeteners, indicating a generalized effect on sweet detection. Neither drug altered responses to non-sweet taste. These findings suggest that pharmacological modulation of KATP channel differently influences sweet taste; closers reduce glucose sensitivity whereas openers attenuate response to multiple sweeteners.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 2","pages":"100082"},"PeriodicalIF":3.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13279012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148239462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting response of polyamine metabolic enzymes underlying fiber-type specific volume regulation in the skeletal muscle with endurance exercise. 多胺代谢酶对骨骼肌纤维型比体积调节的反应与耐力运动的对比。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-07-01 Epub Date: 2026-06-17 DOI: 10.1016/j.jphyss.2026.100083

Maki Yamaguchi, Makiko Ohkido, Naoya Nakahara, Kazuhiro Hirano, Shigeru Morimoto, Michiaki Ikeda, Toshiko Yamazawa, Hideki Yamauchi

{"title":"Contrasting response of polyamine metabolic enzymes underlying fiber-type specific volume regulation in the skeletal muscle with endurance exercise.","authors":"Maki Yamaguchi, Makiko Ohkido, Naoya Nakahara, Kazuhiro Hirano, Shigeru Morimoto, Michiaki Ikeda, Toshiko Yamazawa, Hideki Yamauchi","doi":"10.1016/j.jphyss.2026.100083","DOIUrl":"10.1016/j.jphyss.2026.100083","url":null,"abstract":"To explore the role of polyamine metabolism in muscle-type-specific hypertrophy, we analyzed changes in the expression profiles of polyamine metabolic enzymes and key enzymes for muscle metabolism induced by voluntary wheel running exercise in different muscle types. Effect of the polyamine precursor, putrescine, which was reported to have potential to regulate muscle volume, was also tested. Polyamine synthetic enzymes were upregulated in hypertrophic soleus muscle whereas polyamine catabolic enzymes were upregulated in non-hypertrophic plantar muscle by exercise in correlation with increased mitochondria-related protein expression. The increased catabolic enzymes of polyamines in the plantar muscle were hypothesized to be possibly involved in restriction of hypertrophy in fast-type skeletal muscles correlating with increased aerobic metabolism. Putrescine administration minimally affected polyamine metabolism and muscle volume indicating that it did not effectively regulate muscle hypertrophy. Polyamine oxidase localized in the perinuclear and inter-myofibrillar region suggesting a correlation between aerobic metabolism and polyamine catabolism.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 2","pages":"100083"},"PeriodicalIF":3.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13314752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148295475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological doses of eicosapentaenoic acid suppress high-fat diet-induced atrial fibrillation in mice independent of lipid lowering. 生理剂量的二十碳五烯酸可抑制小鼠高脂肪饮食诱导的心房颤动，不依赖于降脂。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-07-01 Epub Date: 2026-03-27 DOI: 10.1016/j.jphyss.2026.100070

Kosuke Horii, Katsushige Ono, Seiji Masuda, Masaki Morishima

{"title":"Physiological doses of eicosapentaenoic acid suppress high-fat diet-induced atrial fibrillation in mice independent of lipid lowering.","authors":"Kosuke Horii, Katsushige Ono, Seiji Masuda, Masaki Morishima","doi":"10.1016/j.jphyss.2026.100070","DOIUrl":"10.1016/j.jphyss.2026.100070","url":null,"abstract":"Long-term treatment with eicosapentaenoic acid (EPA) has been reported to reduce the risk of atrial fibrillation (AF) in mice fed a high-fat diet (HFD); however, the appropriate dose required to attenuate atrial vulnerability to AF remains unclear. The present study aimed to determine whether different amounts of daily intake of EPA exert preventive effects against AF. We conducted a series of experiments in HFD-fed mice treated with either low-dose EPA (30 mg/kg) or high-dose EPA (300 mg/kg). Both EPA doses similarly shortened AF duration and improved atrial conduction, as assessed by P-wave duration on surface electrocardiography. Notably, neither low- nor high-dose EPA significantly improved HFD-induced dyslipidemia, and no difference in cholesterol-lowering effects was observed between the two EPA treatment groups. These findings suggest that physiologically relevant dietary intake of EPA is sufficient to prevent atrial arrhythmias and to ameliorate impaired atrial conduction associated with AF, independent of systemic lipid-lowering effects.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 2","pages":"100070"},"PeriodicalIF":3.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13068811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147592833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia. TRPM4基因失活不会改变小鼠小胶质细胞的温度依赖性运动。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-07-01 Epub Date: 2026-03-10 DOI: 10.1016/j.jphyss.2026.100067

Rei Nishimoto, Yoshikazu Matsuoka, Makoto Tominaga

引用次数: 0

Involvement of interaction between TRPM2 and IKCA1 in temperature-dependent movement and IL-1β production in mouse microglia. TRPM2和IKCA1在小鼠小胶质细胞温度依赖性运动和IL-1β产生中的相互作用。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-07-01 Epub Date: 2026-05-01 DOI: 10.1016/j.jphyss.2026.100077

Aykut Deveci, Makiko Kashio, Sandra Derouiche, Jing Lei, Yuji Imaizumi, Susumu Ohya, Moe Iwata, Makoto Tominaga

{"title":"Involvement of interaction between TRPM2 and IKCA1 in temperature-dependent movement and IL-1β production in mouse microglia.","authors":"Aykut Deveci, Makiko Kashio, Sandra Derouiche, Jing Lei, Yuji Imaizumi, Susumu Ohya, Moe Iwata, Makoto Tominaga","doi":"10.1016/j.jphyss.2026.100077","DOIUrl":"10.1016/j.jphyss.2026.100077","url":null,"abstract":"Transient potential melastatin 2 (TRPM2) plays important roles in Ca2 + signaling in tissues and cells, and contributes to various cellular functions. The Ca2+ influx induced by TRPM2 may activate the intermediate conductance Ca2+-activated potassium channel (IKCA1/ KCa3.1/ SK4) and trigger K+ efflux. Here we demonstrate that a functional interaction between TRPM2 and IKCA1 contributes to cell volume changes in HEK293T cells and mouse primary microglia. Ca2+ entering cells through TRPM2 causes K+ efflux, followed by cell shrinkage upon water efflux. In addition, mouse microglia exhibited temperature-dependent movement in vitro that was modulated by the TRPM2-IKCA1 interaction within the physiological body temperature range. This interaction was also found to be involved in cytokine production in microglia. Understanding how the TRPM2-IKCA1 interaction in microglia can promote cell movement and cytokine production could be valuable for developing new strategies for treatments of diseases involving TRPM2.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 2","pages":"100077"},"PeriodicalIF":3.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13158756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0