Journal of Physiological Sciences最新文献_第2页

Food allergy-induced anaphylaxis impairs adrenal adrenergic biosynthesis despite preserved vagal afferent-adrenal sympathetic reflex function. 食物过敏引起的过敏反应损害肾上腺素能生物合成，尽管保留迷走神经传入-肾上腺交感反射功能。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-09-06 DOI: 10.1016/j.jphyss.2026.100097

Marina Khatun, Nishat Akther, Sajjad Hossain, Sakurako Asano, Risa Shimane, Nanaka Tameike, Kakeru Shimizu, Yukari Takeda, Mamoru Tanida, Yusaku Iwasaki, Yuta Masuda, Chikara Abe

{"title":"Food allergy-induced anaphylaxis impairs adrenal adrenergic biosynthesis despite preserved vagal afferent-adrenal sympathetic reflex function.","authors":"Marina Khatun, Nishat Akther, Sajjad Hossain, Sakurako Asano, Risa Shimane, Nanaka Tameike, Kakeru Shimizu, Yukari Takeda, Mamoru Tanida, Yusaku Iwasaki, Yuta Masuda, Chikara Abe","doi":"10.1016/j.jphyss.2026.100097","DOIUrl":"10.1016/j.jphyss.2026.100097","url":null,"abstract":"Food-induced anaphylaxis is a life-threatening allergic reaction in which endogenous sympathoadrenal responses play a critical role in maintaining physiological homeostasis. However, the functional status of autonomic pathways regulating adrenal catecholamine output during anaphylaxis remains unclear. Using a mouse model of food allergy-induced anaphylaxis, we examined the vagal afferent-medullary C1 neuron-adrenal sympathetic pathway and adrenal adrenergic function. Ovalbumin challenge induced hypotension, hypothermia, metabolic acidosis, and elevated plasma catecholamines. Despite severe systemic deterioration, vagal afferent stimulation and optogenetic activation of C1 neurons continued to evoke robust adrenal sympathetic responses, indicating preserved autonomic regulation. In contrast, adrenal transcriptomic analysis revealed enrichment of hypoxia-related pathways and selective suppression of phenylethanolamine N-methyltransferase (PNMT), a key enzyme for adrenaline synthesis. Oxygen supplementation partially restored PNMT expression, increased plasma adrenaline levels, and improved physiological outcomes. These findings identify hypoxia-associated adrenal dysfunction as a potential limitation of endogenous adrenergic compensation during anaphylactic shock.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 3","pages":"100097"},"PeriodicalIF":3.3,"publicationDate":"2026-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13579568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional expression of TRPV6 in human syncytiotrophoblasts from trophoblast stem cells - possible role in maternal-fetal calcium transport. TRPV6在滋养细胞干细胞的人合胞滋养细胞中的功能表达——可能在母胎钙运输中起作用。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-08-31 DOI: 10.1016/j.jphyss.2026.100099

Yoshiro Suzuki, Soshi Otani, Shouichi Shibasaki, Keishi Otsu, Katsumi Nakajima

{"title":"Functional expression of TRPV6 in human syncytiotrophoblasts from trophoblast stem cells - possible role in maternal-fetal calcium transport.","authors":"Yoshiro Suzuki, Soshi Otani, Shouichi Shibasaki, Keishi Otsu, Katsumi Nakajima","doi":"10.1016/j.jphyss.2026.100099","DOIUrl":"10.1016/j.jphyss.2026.100099","url":null,"abstract":"Maternal-fetal calcium (Ca2 +) transport through the placenta is crucial for the development of fetal organs including bones. Although we have reported that the transient receptor potential cation channel, subfamily V, member 6 (TRPV6) was responsible for the maternal-fetal Ca2+ transport, there has been no direct evidence for its channel activity in placental trophoblasts. By using human trophoblast stem cells, we observed biphasic higher intracellular Ca2+ levels compared with undifferentiated cells during the differentiation of syncytiotrophoblasts. Second Ca2+ peak, when the syncytiotrophoblasts might mature with transport function, was found to be a Ca2+ flux through TRPV6. Unexpectedly we observed the first peak, when trophoblasts fuse to form syncytiotrophoblast. We found that it was derived through TRPV4, and blocking TRPV4 suppressed trophoblast cell fusion. Our results might provide the first evidence for TRPV6 channel activities in syncytiotrophoblasts involved in the maternal-fetal Ca2+ transport.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 3","pages":"100099"},"PeriodicalIF":3.3,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphorescence lifetime microscopy and contrast-enhanced ultrasound imaging revealed strong left-to-right intraventricular shunt in anesthetized Xenopus laevis. 磷光终身显微镜和超声造影显示麻醉的非洲爪蟾有强烈的左至右脑室分流。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-08-29 DOI: 10.1016/j.jphyss.2026.100098

Ken Hashimoto, Momoko Ohira, Misaki Kimoto, Akiko Oda, Kosuke Tsukada, Akira Hanashima, Satoshi Mohri

{"title":"Phosphorescence lifetime microscopy and contrast-enhanced ultrasound imaging revealed strong left-to-right intraventricular shunt in anesthetized Xenopus laevis.","authors":"Ken Hashimoto, Momoko Ohira, Misaki Kimoto, Akiko Oda, Kosuke Tsukada, Akira Hanashima, Satoshi Mohri","doi":"10.1016/j.jphyss.2026.100098","DOIUrl":"10.1016/j.jphyss.2026.100098","url":null,"abstract":"Amphibians exhibit intracardiac shunting, by which oxygen from multiple respiratory surfaces is variably distributed between systemic and pulmonary circulations. Although shunting has been extensively studied in terrestrial species that rely heavily on pulmonary respiration, much less is known about more aquatic, cutaneous-respiring species, in which cutaneously oxygenated blood returns to the right atrium, so the right-left oxygen gradient should vary with context. Moreover, the proposed role of ventricular trabeculation in limiting intraventricular mixing has not been directly demonstrated. We combined phosphorescence lifetime microscopy for in vivo pO2 measurement with microbubble-based contrast-enhanced ultrasound for high-resolution tracer-tracking in aquatic Xenopus laevis. In anesthetized animals, blood pO2 was uniformly low (25-40 mmHg), with little arteriovenous difference. Right-to-left shunting was minimal (8%), whereas a strong intraventricular left-to-right shunt (47%) maintained threefold greater pulmonary than systemic flow. Although its significance remains to be elucidated, these approaches provide a foundation for advancing shunt research.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 3","pages":"100098"},"PeriodicalIF":3.3,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13570341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148874446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between sensory irritation on skin surface and TRP channel activation. 皮肤表面感觉刺激与TRP通道激活的关系。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-08-27 DOI: 10.1016/j.jphyss.2026.100096

Fumitaka Fujita, Masayuki Takaishi, Shizuka Iwashita, Kiyomi Yoshikawa, Maki Sawada, Hironori Shimizu, Moriaki Nagamatsu, Yuko Shiomi, Takeshi Hara, Kenya Ishida, Kunitoshi Uchida, Yoshiro Suzuki, Makoto Tominaga

{"title":"Relationship between sensory irritation on skin surface and TRP channel activation.","authors":"Fumitaka Fujita, Masayuki Takaishi, Shizuka Iwashita, Kiyomi Yoshikawa, Maki Sawada, Hironori Shimizu, Moriaki Nagamatsu, Yuko Shiomi, Takeshi Hara, Kenya Ishida, Kunitoshi Uchida, Yoshiro Suzuki, Makoto Tominaga","doi":"10.1016/j.jphyss.2026.100096","DOIUrl":"10.1016/j.jphyss.2026.100096","url":null,"abstract":"Mechanisms underlying sensory irritation caused by chemical stimuli on the skin surface remain unknown. Recently, it has been clarified that TRPA1 and TRPV1 are important for sensing nociceptive and temperature stimuli. This study aimed to clarify the mechanism of hTRPA1 and hTRPV1 in sensory irritation on the skin by evaluating the effects of anti-bacterial agents, ethanol, or polyols using electro-physiological methods in HEK293 cells. Parabens (1 mM) activated hTRPA1 in an alkyl chain length-dependent manner. Conversely, high concentrations (>500 mM) of ethanol and polyols activate hTRPV1. The intensities of sensory irritation caused by anti-bacterial agents or polyols correlated with their abilities to activate TRPA1 or TRPV1, respectively. Analogs of known TRPA1 antagonists were screened for their TRPA1 inhibitory activity. Arbanol was identified as a novel TRPA1 inhibitor that can be used to reduce sensory irritation on the skin surface. TRP channels are crucial for sensory irritation on the skin surface.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 3","pages":"100096"},"PeriodicalIF":3.3,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13571459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jinshui Liujun Decoction attenuates cigarette smoke-induced small airway remodeling via the TGF-β/Smad2/CFTR Axis. 金水六君汤通过TGF-β/Smad2/CFTR轴减弱香烟烟雾引起的小气道重塑。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-08-20 DOI: 10.1016/j.jphyss.2026.100095

Yu Liu, Wenjuan Xie, Qijun Zhou, Guo Ouyang

{"title":"Jinshui Liujun Decoction attenuates cigarette smoke-induced small airway remodeling via the TGF-β/Smad2/CFTR Axis.","authors":"Yu Liu, Wenjuan Xie, Qijun Zhou, Guo Ouyang","doi":"10.1016/j.jphyss.2026.100095","DOIUrl":"10.1016/j.jphyss.2026.100095","url":null,"abstract":"Small airway remodeling is a key pathological feature of chronic obstructive pulmonary disease (COPD), and epithelialmesenchymal transition (EMT) is among the central mechanisms driving this remodeling process. Jinshui Liujun Decoction (JSLJD), a traditional Chinese herbal formula, has shown therapeutic potential in respiratory diseases, but its effects on COPD-associated small airway remodeling remain unclear. In this study, JSLJD significantly reduced collagen accumulation, airway wall thickening, and α-SMA expression in the lungs of cigarette smoke (CS)-exposed mice. In vivo and in vitro, JSLJD inhibited EMT, as reflected by elevated E-cadherin expression and reduced vimentin, fibronectin, and Snail expression. Mechanistically, JSLJD inhibited TGF-β/Smad2 activation and restored cystic fibrosis transmembrane conductance regulator (CFTR) expression. Pharmacological blockade of CFTR or activation of TGF-β abrogated the anti-EMT effects of JSLJD. In conclusion, JSLJD alleviates CS-induced small airway remodeling by suppressing EMT via the inhibition of the TGF-β/Smad2 pathway and the recovery of CFTR function, highlighting its promise as a prospective therapy for COPD.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 3","pages":"100095"},"PeriodicalIF":3.3,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13524548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Type-specific and hibernation-associated skeletal muscle remodeling of the back muscle in Syrian hamsters. 叙利亚仓鼠背部肌肉类型特异性和冬眠相关的骨骼肌重塑。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-08-20 DOI: 10.1016/j.jphyss.2026.100093

Nanaka Matsuoka, Akari Yamauchi, Yoshifumi Yamaguchi

引用次数: 0

Simultaneous in vivo imaging of Ca²⁺ signals in periarteriolar cholinergic axonal varicosities and arteriolar diameter changes in the mouse cerebral cortex. 小鼠大脑皮层小动脉周围胆碱能轴索曲张和小动脉直径变化中Ca2+信号的同步体内成像。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-08-19 DOI: 10.1016/j.jphyss.2026.100094

Nobuhiro Watanabe, Harumi Hotta

{"title":"Simultaneous in vivo imaging of Ca2+ signals in periarteriolar cholinergic axonal varicosities and arteriolar diameter changes in the mouse cerebral cortex.","authors":"Nobuhiro Watanabe, Harumi Hotta","doi":"10.1016/j.jphyss.2026.100094","DOIUrl":"10.1016/j.jphyss.2026.100094","url":null,"abstract":"Basal forebrain cholinergic neurons project widely to the cerebral cortex and participate in cerebrovascular regulation. Although cholinergic axons are distributed around the cerebrovasculature, their functional relationship with arteriolar dynamics remains unclear. In this study, we established an in vivo two-photon imaging approach to simultaneously measure Ca²⁺ signals in cholinergic axonal varicosities and arteriolar diameters in urethane-anesthetized mice. An adeno-associated virus (AAV) vector (rAAV-ChAT-jGCaMP8s) was injected into the nucleus basalis of Meynert. In vivo imaging of the frontal cortex revealed bead-shaped GCaMP signals around the arterioles. Pinch stimulation transiently increased Ca²⁺ signals in periarteriolar varicosities, followed by arteriolar dilation, with an approximately 2-s delay between their peaks. Linear regression analysis disclosed a significant relationship between the magnitudes of these changes. This approach enabled simultaneous evaluation of cholinergic axonal activity and arteriolar dynamics in vivo, providing a tool to investigate the cholinergic regulation of cerebrovasculature.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 3","pages":"100094"},"PeriodicalIF":3.3,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148829609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

α-mannosidase I-dependent N-linked glycosylation affects hERG channel functional availability during reactivation. α-甘露糖苷酶i依赖性n -链糖基化影响hERG通道在再激活过程中的功能可用性。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-08-14 DOI: 10.1016/j.jphyss.2026.100092

Mengyan Wei, Hui Sun, Yajuan Yin, Yangong Liu, Pu Wang, Kenshi Yoshimura, Shinichiro Kume, Tatsuki Kurokawa, Katsushige Ono, Ligang Ding, Mingqi Zheng

{"title":"α-mannosidase I-dependent N-linked glycosylation affects hERG channel functional availability during reactivation.","authors":"Mengyan Wei, Hui Sun, Yajuan Yin, Yangong Liu, Pu Wang, Kenshi Yoshimura, Shinichiro Kume, Tatsuki Kurokawa, Katsushige Ono, Ligang Ding, Mingqi Zheng","doi":"10.1016/j.jphyss.2026.100092","DOIUrl":"10.1016/j.jphyss.2026.100092","url":null,"abstract":"Asparagine-linked (N-linked) glycosylation has emerged as an essential post-translational modification to control the number of channels embedded in the plasma membrane as well as to regulate their functional gating properties. The aim of this study was to evaluate the exact role of α-mannosidase I-dependent N-linked glycosylation process in Golgi apparatus on the human rapidly activating delayed rectifier potassium channel, hERG channel. In the heterologous expression system in HEK293 cells, kifunensine, a selective inhibitor of class I α-mannosidase, does not significantly reduce hERG channel current (IhERG) when applied for 48 h. However, kifunensine selectively reduced the reactivation current of the hERG channel, while activation, inactivation and deactivation kinetics were not detectably affected by kifunensine. Our results suggest that α-mannosidase I-dependent N-linked glycosylation in the Golgi apparatus plays a key role in regulating hERG functional availability during reactivation.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 3","pages":"100092"},"PeriodicalIF":3.3,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13524579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential physiological responses to electroacupuncture at ST36 and SP6 in CFA-induced inflammatory arthritis: serum metabolomic evidence for acupoint-specific redox regulation. 在cfa诱导的炎症性关节炎中，电针对ST36和SP6的不同生理反应：穴位特异性氧化还原调节的血清代谢组学证据

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-08-09 DOI: 10.1016/j.jphyss.2026.100091

Seung Heon Jeon, Moongi Ji, Vishnumolakala Sindhuri, Min-Jae Koo, Ki-Tae Ha, Seungtae Kim, Man Jeong Paik, Sungtae Koo

{"title":"Differential physiological responses to electroacupuncture at ST36 and SP6 in CFA-induced inflammatory arthritis: serum metabolomic evidence for acupoint-specific redox regulation.","authors":"Seung Heon Jeon, Moongi Ji, Vishnumolakala Sindhuri, Min-Jae Koo, Ki-Tae Ha, Seungtae Kim, Man Jeong Paik, Sungtae Koo","doi":"10.1016/j.jphyss.2026.100091","DOIUrl":"10.1016/j.jphyss.2026.100091","url":null,"abstract":"Electroacupuncture (EA) modulates neuroimmune and metabolic pathways, but acupoint-specific physiological responses remain poorly characterized. This study investigated acupoint-dependent differences in nociceptive behavior, synovial inflammation, and serum metabolomic profiles in rats with complete Freund's adjuvant (CFA)-induced knee arthritis. Animals were assigned to three groups: CFA without EA, CFA with EA at SP6, and CFA with EA at ST36. EA at ST36 significantly improved hindlimb weight-bearing (74.1% vs. 45.3% at post-injection day 8; p < 0.01), whereas EA at SP6 did not reach significance. Both acupoints comparably reduced synovial inflammation scores. Targeted GC-MS/MS serum metabolomics identified 14 significantly altered metabolites; 2-hydroxybutyric acid (2-HBA) showed the largest fold-change and was selectively normalized by EA at ST36. Intra-articular 2-HBA injection did not alter pain behavior, indicating it represents a systemic physiological consequence of inflammatory-metabolic burden rather than a nociceptive mediator. These findings demonstrate acupoint-specific modulation of redox-metabolic homeostasis by EA in inflammatory arthritis.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"76 3","pages":"100091"},"PeriodicalIF":3.3,"publicationDate":"2026-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13495377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148765281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inter- and intra-site reliability of forearm sweating induced by pilocarpine iontophoresis. 匹罗卡品离子导入诱导前臂出汗的位点间和位点内可靠性。

IF 3.3 4区医学

Journal of Physiological Sciences Pub Date : 2026-07-23 DOI: 10.1016/j.jphyss.2026.100089

Hui Wang, Lu Meng, Junto Otsuka, Hanano Kato, Tatsuro Amano

引用次数: 0