Journal of Pharmacy Research最新文献

{"title":"Chiral separation of sitagliptin phosphate enantiomer by HPLC using amylose based chiral stationary phase","authors":"Bhimireddy Venkata Rami Reddy ,&nbsp;Nanduri Raman ,&nbsp;Badam Sai Kumar ,&nbsp;Chintala Rambabu","doi":"10.1016/j.jopr.2013.06.014","DOIUrl":"10.1016/j.jopr.2013.06.014","url":null,"abstract":"<div><h3>Background</h3><p>Although a number of methods are available for evaluating sitagliptin phosphate (SGP), a common method for separation if its potential enantiomer and its possible impurities with good efficiency remains unavailable. With the objective of developing a method for rapid separation with shorter runtimes, a simple, precise, accurate stability indicating normal phase High-performance liquid chromatographic (NRP-HPLC) coupled with a photodiode array detector method was developed for the quantitative determination of (S)-enantiomer in API substance and as well as drug product.</p></div><div><h3>Methods</h3><p>The proposed novel method uses the mixture of n-heptane-ethanol-diethylamine (DEA) 35:65:0.1 (v/v/v) as a mobile phase. The enantiomer of sitagliptin phosphate was baseline resolved on a Chiralpak AD-H (250 mm×4.6 mm, 5 μm) column. The flow rate of the mobile phase is 1.0 mL/min and the detector wavelength monitored at 265 nm. The developed method was extensively validated.</p></div><div><h3>Result</h3><p>In these conditions, linearity over the concentration range 400-2250 ng/ml for (S)- enantiomer was obtained. The limit of detection and quantification were 150 and 400 ng/ml, respectively. The intra and inter-day precision was less than 1.5%. The recovery of (S)- enantiomer was within 107% in bulk drug.</p></div><div><h3>Conclusion</h3><p>The proposed method was found to be suitable, precise, and accurate for the quantitative determination of (S)-enantiomer in bulk drugs as well as in pharmaceutical formulations.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"7 6","pages":"Pages 546-550"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.06.014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75356413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro determination of sun protection factor and chemical stability of Rosa kordesii extract gel","authors":"Pratik P. Maske,&nbsp;Sachin G. Lokapure,&nbsp;Dhanashri Nimbalkar,&nbsp;Shobharaj Malavi,&nbsp;John I. D'souza","doi":"10.1016/j.jopr.2013.05.021","DOIUrl":"10.1016/j.jopr.2013.05.021","url":null,"abstract":"<div><h3>Aims</h3><p>In the present study, to investigate its chemical stability and the in vitro sun protection factor (SPF) of <em>Rosa kordesii</em> petal extract in a gel formulation.</p></div><div><h3>Methods</h3><p>Due to its antioxidant and photoprotective properties, <em>R. kordesii</em> is a promising candidate for use in cosmetic and pharmaceutical formulations. A high performance liquid chromatography method was used to evaluate the chemical stability using <em>R. kordesii</em> extract as marker at 5, 25 and 45 °C for 3–4 months. The sun protection factors were analyzed by ultraviolet (UV) spectrophotometry using samples irradiated with UVB lamp.</p></div><div><h3>Results</h3><p>The chemical stability of the <em>R. kordesii</em> root extract gel was determined according to the concentration of <em>R. kordesii</em> extracts at different storage temperatures (5, 25 and 45 °C) for 3–4 months. It is screened for in vitro sun protection factor in the <em>R. kordesii</em> extract and of its gel formulation and determines Photostability of the isolated <em>R. kordesii</em> extract and SPF.</p></div><div><h3>Conclusion</h3><p>This study has shown that the <em>R. kordesii</em> petal extract gel is stable for at least 3–4 months when stored at 5 and 25 °C. It is essential for collection of similar data for different plants and their flowers, as well as other parts. This proved activity of plant showed its importance and prophylactic utility in anti-solar formulation. This will be a better, cheaper and safe alternative to harmful chemical sunscreens that used now a days in the industry.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"7 6","pages":"Pages 520-524"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.05.021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87592742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Helicteres isora L. against free radicals, lipid peroxidation, protein oxidation and DNA damage","authors":"Vinay Kumar ,&nbsp;Mansi Sharma ,&nbsp;Melissa Lemos ,&nbsp;Varsha Shriram","doi":"10.1016/j.jopr.2013.05.017","DOIUrl":"10.1016/j.jopr.2013.05.017","url":null,"abstract":"<div><h3>Background</h3><p>Free radicals are implicated with cellular disorders through their detrimental actions on proteins, lipids and DNA and are causative factors for large number of degenerative diseases and aging process. Antioxidants of plant origin hold great significance and have therefore gained utmost importance in recent past. Present investigation was a step in this direction, with an objective to comprehensively evaluate the protective effects of <em>Helicteres isora</em> fruits against free radicals, protein oxidation and DNA damage.</p></div><div><h3>Methods</h3><p>Aqueous (AqE), aqueous-methanol (AqME), methanol (ME) and acetone (AE) extracts of mature pods (fruits) were obtained and concentrated <em>in vacuo.</em> Total phenols, flavonoids, ascorbic acid and carotenoids were estimated from extracts using standard methods. Antioxidant activities of extracts with varying concentrations (200–1000 μg/ml) were determined by total antioxidant activity (TAA), ferric reducing antioxidant power, DPPH, and OH radical scavenging assays besides lipid peroxidation inhibition. Extracts were assessed for protection against AAPH (2,2′-Azobis(2-methylpropionamidine) dihydrochloride) induced-protein oxidation using SDS-PAGE and Fenton's reagent induced-DNA damage using DNA nicking assay.</p></div><div><h3>Results</h3><p>The results postulated that the plant is a rich source of total phenols, flavonoids and ascorbic acid. Extracts showed concentration-dependent free radical scavenging activities and lipid peroxidation inhibition. Amongst all four extracts, AqME showed highest antioxidant potential in terms of reducing power (360 ± 5.9 gallic acid equivalent: GAE), TAA (150 ± 5.6 GAE), scavenging of free radicals including DPPH (75.6%) and OH (100%) besides maximal (97.4%) lipid peroxidation inhibition at 1000 μg/ml concentration. All the extracts barring ME effectively protected the DNA from hydroxyl radical-induced damage. Similarly, fruit extracts effectively protected the AAPH-induced-protein oxidation.</p></div><div><h3>Conclusions</h3><p><em>H. isora</em> fruits exhibited broad-spectrum antioxidant potencies against free radicals and significantly ameliorated various impairments associated with free radical formation including lipid peroxidation, protein oxidation and DNA damage.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 6","pages":"Pages 620-625"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.05.017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76450599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, characterization and antimicrobial activity of pyrimidine based derivatives","authors":"Yogesh Kumar Gupta ,&nbsp;Vinita Gupta ,&nbsp;Sanchita Singh","doi":"10.1016/j.jopr.2013.05.020","DOIUrl":"10.1016/j.jopr.2013.05.020","url":null,"abstract":"<div><h3>Aim</h3><p>Pyrimidine and their derivatives play the vital role in the field of drugs and agricultural <strong>c</strong>hemicals. In recent decades, a large no. of pharmacological studies has been done on Pyrimidine and their derivatives. But still more research is required in order to necessity of biological compounds. “The Chalcone is an aromatic compound that forms the central core for different necessity of biological compounds. The Chalcone is synthesized by an aldol, condensation of 4-methoxy acetophenone with m-phenoxy benzaldehydes in the presence of a catalyst that is treated with thiourea to provide Pyrimidine. The Pyrimidine treated with substituted <em>N</em>-1,3-benzothiazole-2-yl-2-chloro amide gives a compound”.</p></div><div><h3>Methods</h3><p>Elemental analysis, IR, H NMR, C NMR melting point, Thin layer chromatography (TLC) plates (silica gel G) is used to determine the purity of the compounds.</p></div><div><h3>Result</h3><p><strong>Antimicrobial activity:</strong> All the synthesized compounds screened against four different strains, viz two Gram +ve bacteria (<em>Staphylococcus aureus</em> &amp; <em>Streptococcus pyogenes</em>) and two Gram −ve bacteria (<em>Escherichia coli</em> &amp; <em>Pseudomonas aeruginosa</em>), analyzed with standard drugs ampicillin, chloramphenicol, ciprofloxacin, &amp; norfloxacin. <strong>Antifungal activities:</strong> All the synthesized compounds screened against <em>Candida albicans</em>, and <em>Aspergillus niger</em> organisms, analyzed with standard drugs nystatin and griseofulvin.</p></div><div><h3>Conclusion</h3><p>In this paper we focused on the reactions, synthesis, spectral analysis and Microbial activities of Pyrimidine based benzothiazole derivatives. The method gives excellent than previously reported literature. Some of the compounds were effective as antimicrobial and antifungal agents.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"7 6","pages":"Pages 491-495"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.05.020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77339410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of physico–chemical properties of various concentrations of self developed root canal lubricant","authors":"Satish D. Ingale ,&nbsp;Rajendra Kankariya","doi":"10.1016/j.jopr.2013.05.018","DOIUrl":"10.1016/j.jopr.2013.05.018","url":null,"abstract":"<div><h3>Background and aim</h3><p>The major aim of this work is to study the physico–chemical properties of different concentrations of self developed root canal lubricant gel in order to standardize the formulations and to observe its effect on the performance.</p></div><div><h3>Materials and methods</h3><p>The different concentrations of self developed root canal lubricant gel such as 10%, 24% &amp; 31% was prepared and their physico–chemical properties such as appearance, Solid content, 5% aqueous solution pH, Moisture content, B. F. Viscosity and 5% aqueous solution stability etc. are studied.</p></div><div><h3>Results</h3><p>From the experimental study, it was observed that there is no remarkable difference in the physical appearance of the root canal lubricant gel. Solid content goes on increasing and moisture content goes on decreasing as concentration of EDTA increases.</p></div><div><h3>Conclusion</h3><p>The chelating efficiency, cleaning &amp; shaping of root canal goes on increasing as percentage of EDTA increases i.e. solid content increases. Efficiency of lubrication was also found to be better in highly concentrated formulation. B. F. Viscosity of the formulations is always kept similar by adjusting quantity of rheology modifier in order to get good handling characteristics. From 5% aqueous solution stability, it is observed that gel is highly stable.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"7 6","pages":"Pages 502-505"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.05.018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88164666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zea mays leaf extracts protects primary chick embryo fibroblast cells from apoptosis induced by hydrogen peroxide in vitro","authors":"Balasubramanian Kiruthika ,&nbsp;Sudha Devi Manickam ,&nbsp;Palghat Raghunathan Padma","doi":"10.1016/j.jopr.2013.06.009","DOIUrl":"10.1016/j.jopr.2013.06.009","url":null,"abstract":"<div><h3>Background</h3><p>It is known that excessive oxidative damage, when unchecked, can result in cell death. Therefore, in the present study, the extent of cell death induced by H₂O₂-induced oxidative stress in primary cultured chick embryo fibroblasts and its protection by <em>Zea mays</em> leaf extracts was followed.</p></div><div><h3>Methods</h3><p>Various apoptosis related parameters like cell viability, morphological changes, nuclear changes and apoptotic index were characterized. SRB and MTT assays were used to quantify the extent of cell death in the group exposed to H₂O₂, plant extracts and their combination.</p></div><div><h3>Results and discussion</h3><p>The treatment with hydrogen peroxide exhibited cytotoxicity in the primary chick embryo fibroblasts cells. The number of apoptotic cells increased in the oxidant treated groups. When administered along with H₂O₂, the leaf extracts resulted in a significantly decreased number of apoptotic cells. The maximum inhibition of H₂O₂-induced apoptosis was exhibited by the methanolic extract followed by the aqueous and chloroform extracts.</p></div><div><h3>Conclusion</h3><p>These results indicate that the <em>Zea mays</em> leaves can render protection to chick embryo fibroblasts against H₂O₂-induced cell death.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 6","pages":"Pages 638-646"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.06.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73072032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and in-vivo characterization of novel trans buccal formulations of Amiloride hydrochloride","authors":"J. Ravi Kumar Reddy ,&nbsp;Y. Indira Muzib ,&nbsp;K.P.R. Chowdary","doi":"10.1016/j.jopr.2013.04.051","DOIUrl":"10.1016/j.jopr.2013.04.051","url":null,"abstract":"<div><h3>Objectives</h3><p>Formulation development and characterization of novel trans-buccoadhesive patches and bilayer tablets of Amiloride hydrochloride (AMHCL) with the objectives to avoid the first-pass effect, incomplete absorption from GIT, improve the bioavailability, minimize the dose, improve the duration of action and hence produce controlled drug delivery.</p></div><div><h3>Methods</h3><p>AMHCL patches were prepared by solvent casting method, using hydroxy propyl methyl cellulose (HPMC), Carbopol, Chitosan, and polyvinylpyrrolidone-K30 (PVP). Tablets were prepared by direct compression method, using sodium carboxy methyl cellulose (SCMC), HPMC K100, sodium alginate, Carbopol 934 P, Eudragit RL 100, PVP and ethyl cellulose (EC) as a backing layer. The both formulations were evaluated for their thickness uniformity, folding endurance, weight uniformity, content uniformity, swelling behavior, tensile strength, buccoadhesive strength, surface pH and <em>in vitro</em> release studies.</p></div><div><h3>Results</h3><p>Data of <em>in vitro</em> release from both patches and tablets were fit to different equations and kinetic models to explain release profiles. <em>In vivo</em> drug release studies in rabbits showed 91.65% of drug release from HPMC-Chitosan patch, while it was 82.63–90.21% release from sodium alginate-SCMC buccal tablets. Good correlation among <em>in vitro</em> release and <em>in vivo</em> release of AMHCL was observed in both formulations.</p></div><div><h3>Conclusion</h3><p>The satisfactory results were obtained in all prepared formulations and based on the results, it can be concluded, Amiloride hydrochloride oral mucoadhesive buccal formulations which can be used mainly in minimizing dose and mainly help to improve the patient compliance and Amiloride hydrochloride is a drug of choice for delivery through the control release via buccal route.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 6","pages":"Pages 647-652"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.04.051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82429355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and evaluation of glipizide CR tablets employing starch acetate as rate controlling matrix former","authors":"P. Seenivasan ,&nbsp;K.P.R. Chowdary ,&nbsp;C. Uma Maheswara Reddy ,&nbsp;J.S.N. Murthy","doi":"10.1016/j.jopr.2013.06.013","DOIUrl":"10.1016/j.jopr.2013.06.013","url":null,"abstract":"<div><h3>Background/Objectives</h3><p>In the present work, starch acetate was synthesized, characterized and evaluated as effective release retarding matrix materials.</p></div><div><h3>Methods</h3><p>Matrix tablets of glipizide were formulated employing starch acetate in different proportions of drug and polymer and the tablets were evaluated for drug release kinetics and mechanism.</p></div><div><h3>Results</h3><p>Starch acetate (SA) was prepared by acetylation of potato starch with acetic anhydride in alkaline medium. The percent acetylation was 42.38% and the degree of substitution was 2.75. Glipizide (10 mg) matrix tablets prepared using starch acetate as matrix former in different concentrations gave slow and controlled release more than 24 h. Glipizide release from the tablets formulated was slow and depends on the concentration (%) of starch acetate in the matrix tablets and nature/type of diluent. A linear relationship was found out between percent of polymer, starch acetate and release rate (<em>K</em>₀) of the tablets. The controlled release characteristics of the starch acetate matrix tablets of the drug remained unaltered during short time accelerated stability study.</p></div><div><h3>Conclusions</h3><p>Starch acetate was found suitable as matrix former for controlled release and the matrix tablets of glipizide formulated employing starch acetate gave controlled release of glipizide over 24 h.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 6","pages":"Pages 653-655"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.06.013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74808892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tab-in-tab formulation of ramipril and nifedipine for effective anti-hypertensive activity","authors":"Pravin K. Shende ,&nbsp;Vedraj V. Vellody ,&nbsp;R.S. Gaud","doi":"10.1016/j.jopr.2013.06.008","DOIUrl":"10.1016/j.jopr.2013.06.008","url":null,"abstract":"<div><h3>Background</h3><p>The aims of this work were to enhance the solubility of nifedipine in acidic medium; to formulate and characterize tab-in-tab dosage form for immediate release of nifedipine and controlled release of ramipril and to avoid the mutual interactions of drugs. This combination appreciably intended to reduce the incidence of peripheral edema and reflex tachycardia.</p></div><div><h3>Methods</h3><p>Ramipril was firstly wet granulated with a HPMC polymer to form stable granules and then was directly compressed to form a core tablet. Enteric coating was given with a water insoluble polymer to withstand the acidic pH of stomach. Initially, nifedipine microcapsules were prepared, granulated with excipients and then compressed, keeping the inner tablet in between the granules.</p></div><div><h3>Results and discussion</h3><p>This novel tab-in-tab dosage form allowed the nifedipine to release for stomach-specific absorption and ramipril to the intestine-specific absorption. Distinctive release profiles were obtained for immediate- and controlled-release tab-in-tab formulations, giving better bioavailability with no metabolic side-effects.</p></div><div><h3>Conclusion</h3><p>Tab-in-tab drug delivery system used to formulate combination drugs with different pharmacokinetic profiles provide reduction in dosage, dosing frequency, reduction in side effects, additive effects and single pill convenience.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 6","pages":"Pages 656-662"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.06.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88247527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro antioxidant activity and in vivo alpha glucosidase activity of endophytic actinomycetes isolated from Catharanthus roseus (l.) G. Don","authors":"D. Jacintha Jasmine,&nbsp;P. Agastian","doi":"10.1016/j.jopr.2013.06.007","DOIUrl":"10.1016/j.jopr.2013.06.007","url":null,"abstract":"<div><h3>Aim</h3><p>Antioxidant compounds in food play an important role as a health protecting factor. Scientific evidence suggests that antioxidants reduce the risk for chronic diseases including cancer and heart disease. The present study was to evaluate the free radical scavenging activity and alpha glucosidase activity of Ethyl acetate extracts of endophytic actinomycetes (EEA) isolated from the roots of <em>Catharanthus roseus</em> (l.) G. Don.</p></div><div><h3>Method</h3><p>The EEA was screened for its capability in lowering blood sugar level and radical scavenging activity against Hydroxyl (HO^-), Hydrogen peroxide (H₂O₂), Nitric oxide (NO) and scavenging of stable radical 2,2-diphenyl-1-picrylhydrazyl or DPPH and using standard procedures.</p></div><div><h3>Result</h3><p>Significant free radical scavenging activity and marked alpha glucosidase activity were exhibited by EEA isolated from the roots of <em>Catharanthus roseus</em> (l.) G. Don.</p></div><div><h3>Conclusion</h3><p>The extract used in the study possesses significant antioxidant activity as well as an alpha glucosidase inhibition and have the potential for application in pharmaceuticals.</p></div>","PeriodicalId":16787,"journal":{"name":"Journal of Pharmacy Research","volume":"6 6","pages":"Pages 674-678"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jopr.2013.06.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80588563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}