Journal of Pharmacopuncture最新文献

{"title":"Preliminary Mechanistic Study on the Trachea Smooth Muscle Relaxant Activity of Aqueous Leaf Extract of <i>Tridax Procumbens</i> in Male Wistar Rats.","authors":"Shakiru Ademola Salami,&nbsp;Hussein Mofomosara Salahdeen,&nbsp;Babatunde Shuaib Anidu,&nbsp;Babatunde Adekunle Murtala,&nbsp;AbdulRasak Akinola Alada","doi":"10.3831/KPI.2022.25.3.209","DOIUrl":"https://doi.org/10.3831/KPI.2022.25.3.209","url":null,"abstract":"<p><strong>Objectives: </strong>Aqueous leaf extract of <i>Tridax procumbens</i> (ALETP) has potent relaxant activity. However, this relaxant activity in respiratory smooth muscle remains uninvestigated. This study investigates the effect of ALETP on the contractile activity of tracheal smooth muscle (TSM) in adult male Wistar rats.</p><p><strong>Methods: </strong>Twelve male Wistar rats divided into 2 groups and were treated with either 100 mg/kg of ALETP (ALETP treatment group) or vehicle (distilled water; control group) through oral gavage for 4 weeks. Dose responses of TSM from the 2 groups to acetylcholine (10^-9 to 10^-5 M), phenylephrine (10^-9 to 10^-5 M), and potassium chloride (KCl; 10^-9 to 10^-4 M) were determined cumulatively. Furthermore, cumulative dose responses to acetylcholine (10^-9 to 10^-5 M) after pre-incubation of TSM with atropine (10^-5 M), L-NAME (10^-4 M), indomethacin (10^-4 M), and nifedipine (10^-4 M), were determined.</p><p><strong>Results: </strong>Treatment with ALETP substantially inhibited TSM contraction stimulated by cumulative doses of acetylcholine, phenylephrine, and KCl. Furthermore, preincubation of TSM from the 2 groups in atropine significantly inhibited contractility in TSM. Incubation in L-NAME and indomethacin also significantly inhibited contractility in TSM of ALETP-treated rats compared to that of controls. Contractile activity of the TSM was also inhibited significantly with incubation in nifedipine in ALETP-treated rats.</p><p><strong>Conclusion: </strong>ALETP enhanced relaxant activity in rat TSM primarily by blocking the L-type calcium channel and promoting endothelial nitric oxide release. ALETP contains agents that may be useful in disorders of the respiratory tract.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 3","pages":"209-215"},"PeriodicalIF":1.4,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/af/fd/jop-25-3-209.PMC9510139.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40390123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Overview on Plant Derived Phenolic Compounds and Their Role in Treatment and Management of Diabetes.","authors":"Himangshu Deka,&nbsp;Ananta Choudhury,&nbsp;Biplab Kumar Dey","doi":"10.3831/KPI.2022.25.3.199","DOIUrl":"https://doi.org/10.3831/KPI.2022.25.3.199","url":null,"abstract":"<p><strong>Objectives: </strong>In recent decades, the trend for treating diabetes mellitus (DM) has shifted toward alternative medicines that are obtained from plant sources. Existing literature suggests that phenolic compounds derived from plants possess promising health-promoting properties. This study aimed to discuss the role of plant-derived phenolic compounds in the effective treatment and management of diabetes.</p><p><strong>Methods: </strong>Information about plant secondary metabolites, phenolic compounds, and their role in the treatment and management of diabetes was collected from different databases, such as Pubmed, ScienceDirect, Scopus, and Google Scholar. Keywords like secondary metabolites, phenolic compounds, simple phenol, flavonoids, lignans, stilbenes, and diabetes were searched. Research and review articles with relevant information were included in the study.</p><p><strong>Results: </strong>Anti-diabetic studies of the four major classes of phenolic compounds were included in this review. The plant-derived phenolic compounds were reported to have potent anti-diabetic activities. However, each class of phenolic compounds was found to behave differently according to various mechanisms.</p><p><strong>Conclusion: </strong>The obtained results suggest that phenolic compounds derived from natural sources display promising anti-diabetic activities. Based on the available information, it can be concluded that phenolic compounds obtained from various natural sources play key roles in the treatment and management of diabetes.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 3","pages":"199-208"},"PeriodicalIF":1.4,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/11/33/jop-25-3-199.PMC9510143.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40390707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Acupuncture, Mind and Body Practices, and Natural Products for Insomnia: an overview of systematic reviews.","authors":"Jin Ju Yeo, Jang Won Lee, Kyung Sik Kim, Min Kyung Hyun","doi":"10.3831/KPI.2022.25.3.186","DOIUrl":"10.3831/KPI.2022.25.3.186","url":null,"abstract":"<p><strong>Objectives: </strong>This overview summarizes the evidence for the effectiveness and safety of complementary therapies for insomnia through systematic reviews (SRs) and meta-analyses (MAs).</p><p><strong>Methods: </strong>A comprehensive literature search on SRs and MAs for complementary therapies for insomnia was conducted using six databases.</p><p><strong>Results: </strong>This overview included 30 SRs/MAs. The Pittsburgh Sleep Quality Index (PSQI) analysis of insomnia included 383 randomized controlled trials and 31,748 participants. Twenty-four SRs/MAs (80%) out of 30 SRs/MAs reported a positive result, and six SRs/MAs reported a partially positive result. The most frequently used form of acupuncture reported a positive effect in 89.5% (17/19) of SRs/MAs and a superior effect to western medicine, sham/placebo, and no treatment. The lack of protocol registration and the excluded studies list resulted in generally poor methodological and reporting quality in SRs/MAs with AMSTAR 2.</p><p><strong>Conclusion: </strong>This overview confirmed that complementary therapies positively affected the PSQI scores in patients with insomnia. However, better-designed primary studies are needed to strengthen the relevant evidence in the future. A more stringent assessment of multiple systematic reviews 2 must be followed when performing SR and MA.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 3","pages":"186-198"},"PeriodicalIF":1.2,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/be/96/jop-25-3-186.PMC9510140.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40390126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactive Effects of Nutrition Modification and Wet Cupping on Male Patients with Refractory Stable Angina.","authors":"Mohammad Abazari,&nbsp;Mahdi Yousefi,&nbsp;Vafa Baradaran Rahimi,&nbsp;Hamideh Vahid,&nbsp;Azadeh Saki,&nbsp;Arash Gholoobi","doi":"10.3831/KPI.2022.25.3.224","DOIUrl":"https://doi.org/10.3831/KPI.2022.25.3.224","url":null,"abstract":"<p><strong>Objectives: </strong>Routine therapies cannot control refractory stable angina, leading to a high economic burden and an impaired quality of life. Persian medicine incorporates exceptional attention to lifestyle and nutrition to prevent and treat various diseases. Previous studies have reported the analgesic and anti-inflammatory effects of wet cupping. The present study aims to determine the effects of nutrition and cupping on refractory stable angina patients.</p><p><strong>Methods: </strong>Forty male patients with refractory stable angina were randomly allocated to four groups, including nutrition modification based on Persian medicine, wet cupping, nutrition modification along with wet cupping, and control. The primary outcomes were the changes in pain score using a visual analog scale and quality of life using the Seattle angina questionnaire. The secondary outcomes were changes in the exercise test and blood pressure.</p><p><strong>Results: </strong>The results of the present study revealed that 30 days of treatment with nutrition modification based on Persian medicine, cupping, and modified nutrition and cupping along with standard treatment for stable angina significantly increased the patient's quality of life and exercise test results while reducing pain, and systolic and diastolic blood pressure.</p><p><strong>Conclusion: </strong>Applying complementary Persian medicine methods such as nutrition modification and cupping along with the classical medical treatments may improve outcomes for refractory stable angina patients.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 3","pages":"224-232"},"PeriodicalIF":1.4,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8e/57/jop-25-3-224.PMC9510135.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40390127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Genotoxicity of SU-Eohyeol Pharmacopuncture Using an In Vitro Chromosome Aberration Test in Chinese Hamster Lung Cell.","authors":"Jaseung Ku,&nbsp;Ji Hye Hwang","doi":"10.3831/KPI.2022.25.3.290","DOIUrl":"10.3831/KPI.2022.25.3.290","url":null,"abstract":"<p><strong>Objectives: </strong>This study was conducted to evaluate the safety of SU-Eohyeol pharmacopuncture (SUEP) by assessing its potential to cause chromosomal abnormalities in Chinese hamster lung cells (CHL/IC).</p><p><strong>Methods: </strong>A dose-curve was conducted to determine the highest dose of SUEP. Doses of 10, 5, 2.5, 1.25, 0.625, and 0.313% were used, and no cytotoxicity or SUEP precipitation was observed. SUEP doses of 10, 5, and 2.5%, with positive and negative controls, were used in a chromosome aberration test.</p><p><strong>Results: </strong>In this study, the frequency of abnormal chromosomal cells in the SUEP group did not show a statistically significant difference from that of the negative control group in short-term treatments with and without metabolic activation and the continuous treatment without metabolic activation. Compared with the negative control group, the positive control group had a significantly higher frequency of cells with structural chromosomal abnormalities. This test's results satisfied all conditions for determining the results.</p><p><strong>Conclusion: </strong>SUEP did not induce chromosomal aberrations under the conditions of this study. Other toxicity evaluations, safety studies in humans, and various clinical trials are required to evaluate the safety and efficacy of SUEP.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 3","pages":"290-300"},"PeriodicalIF":1.4,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/12/2c/jop-25-3-290.PMC9510136.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40390704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Antioxidant, Hypoglycemic and Anti-Obesity Effects of Euphorbia Resinifera L.","authors":"Kaoutar Benrahou,&nbsp;Otman El Guourrami,&nbsp;Hanae Naceiri Mrabti,&nbsp;Yahia Cherrah,&nbsp;My El Abbes Faouzi","doi":"10.3831/KPI.2022.25.3.242","DOIUrl":"https://doi.org/10.3831/KPI.2022.25.3.242","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this work is to evaluate the in vitro antioxidant, hypoglycemic, and antiobesity effects of Euphorbia resinifera extracts and investigate the phenolic constituents and the toxicity of these extracts.</p><p><strong>Methods: </strong>Phytochemical screening was performed to detect polyphenols and flavonoids. Antioxidant activity was evaluated by four methods (DPPH, ABTS, H₂O₂, and xanthine oxidase inhibition). The hypoglycemic effect was determined by the inhibition of α-amylase and α-glucosidase enzymes in vitro and via a starch tolerance study in normal rats. The antiobesity effect was estimated by in vitro inhibition of lipase.</p><p><strong>Results: </strong>Phytochemical screening revealed that the ethanolic extract was rich in polyphenols (99 ± 0.56 mg GEA/g extract) and tannins (55.22 ± 0.17 mg RE/g extract). Moreover, this extract showed higher antioxidant activity in different tests the DPPH assay (IC₅₀ = 53.81 ± 1.83 µg/mL), ABTS assay (111.4 ± 2.64 mg TE/g extract), H₂O₂ (IC₅₀ = 98.15 ± 0.68 µg/mL), and xanthine oxidase (IC₅₀ = 10.26 ± 0.6 µg/mL). With respect to hypoglycemic effect, the aqueous and ethanolic extracts showed IC₅₀ values of 119.7 ± 2.15 µg/mL and 102 ± 3.63 µg/mL for α-amylase and 121.4 ± 1.88 and 56.6 ± 1.12 µg/mL for α-glucosidase, respectively, and the extracts lowered blood glucose levels in normal starch-loaded rats. Additionally, lipase inhibition was observed with aqueous (IC₅₀ = 25.3 ± 1.53 µg/mL) and ethanolic (IC₅₀ = 13.7 ± 3.03 µg/mL) extracts.</p><p><strong>Conclusion: </strong>These findings show the antioxidant, hypoglycemic, and hyperlipidemic effects of E. resinifera extracts, which should be investigated further to validate their medicinal uses and their pharmaceutical applications.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 3","pages":"242-249"},"PeriodicalIF":1.4,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/38/56/jop-25-3-242.PMC9510134.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40390702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnomedicinal Practices and Traditional Medicinal Plants of Barak Valley, Assam: a systematic review.","authors":"Pervej Alom Barbhuiya,&nbsp;Abdul Mannaf Laskar,&nbsp;Hemanga Mazumdar,&nbsp;Partha Pratim Dutta,&nbsp;Manash Pratim Pathak,&nbsp;Biplab Kumar Dey,&nbsp;Saikat Sen","doi":"10.3831/KPI.2022.25.3.","DOIUrl":"https://doi.org/10.3831/KPI.2022.25.3.","url":null,"abstract":"<p><strong>Objectives: </strong>The Barak Valley is well known for its rich diversity of medicinal plants. Ethnomedicinal practices are prominent among Barak Valley's major and minor ethnic groups. This systemic review focuses on traditionally used medicinal plants found in the Barak Valley as reported in different ethnobotanical surveys.</p><p><strong>Methods: </strong>We searched various databases, including PubMed, Web of Science, and Google Scholar, to find ethnomedicinal surveys conducted in the Barak Valley. The search was performed using different terms, including ethnomedicinal survey, folk medicine, indigenous knowledge, and Barak Valley. Potential articles were identified following the exclusion and inclusion criteria.</p><p><strong>Results: </strong>A total of eight ethnobotanical surveys were included in this study. We identified a total of 216 plant species belonging to 167 genera and 87 families, which are widely used by the ethnic communities who live in the rural areas of Barak Valley for the treatment of various diseases and ailments.</p><p><strong>Conclusion: </strong>Folk medicine is the result of decades of accumulated knowledge and practices by people who live in rural communities based on their needs and provides an important source of information to assist the search for new pharmaceuticals. Therefore, available information on traditional medicinal plants needs to be explored scientifically to find effective and alternative treatments for different diseases.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 3","pages":"149-185"},"PeriodicalIF":1.4,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/97/35/jop-25-3-149.PMC9510141.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40390128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroalcoholic Extract of <i>Scrophularia Striata</i> Attenuates Hypertrophic Scar, Suppresses Collagen Synthesis, and Stimulates MMP2 and 9 Gene Expression in Rabbit Ear Model.","authors":"Hatam Zarei,&nbsp;Pari Tamri,&nbsp;Sara Soleimani Asl,&nbsp;Meysam Soleimani,&nbsp;Shirin Moradkhani","doi":"10.3831/KPI.2022.25.3.258","DOIUrl":"https://doi.org/10.3831/KPI.2022.25.3.258","url":null,"abstract":"<p><strong>Objectives: </strong>Hypertrophic scars (HSs) are caused by abnormal wound healing. To date, no standard treatment has been made available for HSs. <i>Scrophularia striata</i> has been reported to accelerate wound healing and has the potential to prevent HS formation. In this study, we investigated the anti-scarring effects of <i>S. striata</i> extract (SSE) in a rabbit ear model of scarring.</p><p><strong>Methods: </strong>In this study, New Zealand white rabbit (weight 2.3-2.5 kg) were used. In the prevention phase of the study, three test groups received 5%, 10%, and 15% ointments of SSE in the Eucerin base, the fourth group received Eucerin, and the fifth group received no treatment. The samples were obtained on day 35 after wounding. In the treatment phase of the study, the test groups received an intralesional injection of SSE (5%, 10%, and 15%), the fourth group received an intralesional injection of triamcinolone, the fifth group received a solvent (injection vehicle), and the sixth group received no treatment. To evaluate the anti-scarring effects of SSE, the scar elevation index (SEI), epidermis thickness index (ETI), collagen deposition, and MMP2 and MMP9 gene expression were evaluated.</p><p><strong>Results: </strong>A significant reduction in SEI, ETI, and collagen deposition was noted in animals treated with SSE compared with the control groups. In addition, topical SSE stimulated MMP2 and MMP9 gene expression.</p><p><strong>Conclusion: </strong>The findings of this study demonstrate the potential for SSE in the prevention and treatment of HS. SSE could be prepared as an appropriate formulation to treat wounds and prevent abnormal scarring.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 3","pages":"258-267"},"PeriodicalIF":1.4,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/23/5b/jop-25-3-258.PMC9510145.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40390705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Treatment Patterns of Sleep Disorders in the Under 20 Population: analysis using a national health insurance claims database.","authors":"Jang Won Lee,&nbsp;Jinseub Hwang,&nbsp;Min Kyung Hyun","doi":"10.3831/KPI.2022.25.3.276","DOIUrl":"https://doi.org/10.3831/KPI.2022.25.3.276","url":null,"abstract":"<p><strong>Objectives: </strong>Child and adolescent sleep is an important factor for brain and physical development. Therefore, it is necessary to investigate the prevalence of sleep disorders and nonorganic sleep disorders in children and adolescents and determine the type of utilization of medical institutions. This study analyzed the prevalence and type of medical institutions in Korean children and adolescents with sleep disorders and nonorganic sleep disorders.</p><p><strong>Methods: </strong>This study used data recorded in the Health Insurance Review and Assessment-National Patient Sample (HIRA-NPS) database from 2010 to 2017. Details of medical institution type and patient's sex, age, and treatment type were extracted for patients younger than 20 years with sleep disorders and nonorganic sleep disorders.</p><p><strong>Results: </strong>Among 2,536,478 patients under age 20, we identified 3,772 patients with sleep disorders or nonorganic sleep disorders. From 2010 to 2017, the prevalence of sleep disorders in children and adolescents was 0.07% to 0.09%. The utilization rate of Korean medical institutions was 30.47%. The prevalence of nonorganic sleep disorders and the utilization rate of Korean medical institutions were 0.06% to 0.08% and 45.99%, respectively.</p><p><strong>Conclusion: </strong>The prevalence of sleep disorders and nonorganic sleep disorders in the under-20 population was 0.14% to 0.16%. More than 70% of patients with nonorganic sleep disorder who were younger than 9 years used Korean medical institutions.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 3","pages":"276-289"},"PeriodicalIF":1.4,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/61/2f/jop-25-3-276.PMC9510137.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40390124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective Effect of Root Extracts of Berberis Vulgaris (Barberry) on Oxidative Stress on SH-SY5Y Cells","authors":"Elham Shahriari Rad, A. Eidi, D. Minai-Tehrani, S. Bonakdar, S. Shoeibi","doi":"10.3831/KPI.2022.25.3.216","DOIUrl":"https://doi.org/10.3831/KPI.2022.25.3.216","url":null,"abstract":"Objectives Oxidative stress plays a key role in chronic and acute brain disorders and neuronal damage associated with Alzheimer disease (AD) and other neurodegeneration symptoms. The neuroprotective effects of berberine and Berberis vulgaris (barberry) root extract against apoptosis induced by hydrogen peroxide (H2O2) in the human SH-SY5Y cell line were studied. Methods The methanolic extraction of barberry root was performed using a maceration procedure. Oxidative stress was induced in SH-SY5Y cells by H2O2, and an MTT assay was applied to evaluate the neuroprotective effects of berberine and barberry root extract. The cells were pretreated with the half maximal inhibitory concentration (IC50) of each compound (including berberine, barberry root extract, and H2O2), and the anti-apoptotic effects of all components were investigated using RT-PCR. Results The SH-SY5Y cell viability increased in both groups exposed to 75 and 150 ppm barberry extract compared with that in the H2O2-treated group. The data showed that exposing SH-SY5Y cells to 30 ppm berberine significantly increased the cell viability compared with the H2O2-treated group; treatment with 150 and 300 ppm berberine and H2O2 significantly decreased the SH-SY5Y cell viability and was associated with berberine cytotoxicity. The mRNA levels of Bax decreased significantly under treatment with berberine at 30 ppm compared with the control group. A significant increase in Bcl-2 expression was observed only after treatment with the IC50 of berberine. The expression level of Bcl-2 in cells exposed to both berberine and barberry extracts was also significantly higher than that in cells exposed to H2O2. Conclusion The outcomes of this study suggest that treatment of SH-SY5Y cells with barberry extract and berberine could suppress apoptosis by regulating the actions of Bcl-2 family members.","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 1","pages":"216 - 223"},"PeriodicalIF":1.4,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41841731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}