Journal of Pharmacopuncture最新文献

{"title":"Home Healthcare Service Awareness Survey for Korean Medicine Doctors: a survey study.","authors":"Hye In Jeong,&nbsp;Taegwang Nam,&nbsp;Minhui Hong,&nbsp;Kyeong Han Kim","doi":"10.3831/KPI.2023.26.1.60","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.1.60","url":null,"abstract":"<p><strong>Objectives: </strong>Discussions regarding \"medical blind spots\" in Korea's \"aging society\" are continuously rising. In addition, the demand for medical attention and care for the elderly and vulnerable populations continues to increase. Given this, the government is promoting the \"home healthcare service\" project. This study aims to lay the foundation for promoting this project by investigating the perception of clinical Korean Medicine (KM) doctors in the \"community health care\" project.</p><p><strong>Methods: </strong>With the cooperation of the Association of Korean Medicine, we sent a questionnaire to all KM doctors through e-mail. The survey included personal information, awareness, appropriate disease and intervention, proper visit location, and pros and cons.</p><p><strong>Results: </strong>A total of 602 responses were collected and analyzed. Approximately 20% of the doctors answered that they were well aware of the service, while 55% responded that they did not know about it. For a visit, a KM doctor selected the appropriate diseases in the order of stroke, dementia and Parkinson's disease, osteoarthritis, and chronic diseases. Among treatments, acupuncture, moxibustion, and herbal medicine exhibited similar results. The most common opinion was that KM doctors should schedule their visits once a week for 6-12 months, which was the most prolonged period among the given options. More than 80% (84.1%) of the doctors replied that care projects were highly essential, and about 63.8% expressed their willingness to participate in these projects.</p><p><strong>Conclusion: </strong>To provide appropriate home health care, we must raise awareness among Korean medicine doctors. In addition, the healthcare budget must be increased to provide the required support.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 1","pages":"60-66"},"PeriodicalIF":1.4,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4f/b2/jop-26-1-60.PMC10063417.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9240915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of Clinical and Preclinical Studies on the Therapeutic Potential of Black Seeds (<i>Nigella sativa</i>) in the Management of Polycystic Ovarian Syndrome (PCOS).","authors":"Rajkapoor Balasubramanian,&nbsp;Naina Mohamed Pakkir Maideen,&nbsp;Sudha Muthusamy,&nbsp;Mirunalini Gobinath","doi":"10.3831/KPI.2023.26.1.1","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.1.1","url":null,"abstract":"<p><strong>Objectives: </strong>Polycystic ovary syndrome (PCOS) is a condition that occurs frequently among women of reproductive age and is a polygenic, multifactorial, endocrine, and metabolic disorder. PCOS is becoming more common as a result of risk factors such as current lifestyle, overnutrition, and stress. The use of traditional herbal medicine is higher among the global population. Hence, this review article focuses on the potential of <i>Nigella sativa</i> to manage women with PCOS.</p><p><strong>Methods: </strong>A literature search was carried out using databases including Medline, Google Scholar, EBSCO, Embase, and Science Direct, as well as reference lists, to identify relevant publications that support the use of <i>N. sativa</i> in the management of women with PCOS.</p><p><strong>Results: </strong>Several clinical and preclinical studies have demonstrated that the major bioactive constituent of black seed (<i>N. sativa</i>), thymoquinone, has potential for managing women with PCOS. Moreover, <i>N. sativa</i> may help to manage oligomenorrhea and amenorrhea in women with PCOS through its anti-inflammatory and antioxidant properties.</p><p><strong>Conclusion: </strong><i>N. sativa</i> has potential for use as a herbal medicine for managing women with PCOS as an integrative medicine along with traditional and modern medicine in conjunction with calorie restriction and regular exercise.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 1","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/f1/jop-26-1-1.PMC10063426.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9240917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suggested Integrative Approach for Hand Abscess with Cupping Therapy: a case study.","authors":"Emad Ahmed Fathy Hussein,&nbsp;Shahira Hassan Ibrahim Negm,&nbsp;Tabish Ishaq Shaikh,&nbsp;Ahmed Helmy Saleh","doi":"10.3831/KPI.2023.26.1.94","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.1.94","url":null,"abstract":"<p><p>A forty-three-year-old male patient was diagnosed with an acute abscess in the dorsum of the right hand. On the 5th day of conventional pharmacological therapy the patient was still suffering, and was referred to the Outpatient department (OPD) to evacuate and drain the abscess and treat the edema around the area with Hijama (wet cupping therapy, WCT). The hand abscess was successfully cured within a week using an integrative approach of wet cupping therapy together with conventional drug therapy.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 1","pages":"94-98"},"PeriodicalIF":1.4,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8d/6a/jop-26-1-94.PMC10063414.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9246833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Dose Intramuscular Toxicity Test Using No-Pain Pharmacopuncture in Sprague-Dawley Rats.","authors":"Ji Hye Hwang,&nbsp;Chul Jung","doi":"10.3831/KPI.2023.26.1.86","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.1.86","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to evaluate the potential toxicity of a recently developed and clinically used No-Pain pharmacopuncture (NPP) solution. We also assessed the lethal dose of the NPP agent following a single intramuscular injection in Sprague-Dawley (SD) rats.</p><p><strong>Methods: </strong>Animals were divided into two groups the NPP test material group and the normal saline control group. A single intramuscular injection of the NPP agent (1.0 mL/animal) was administered to rats of the NPP test material group. The control group rats received the same volume of normal saline. Both female and male rats were included in each group. All rats were monitored for clinical signs and body weight changes for 14 days after administration of the test substance or saline. At the end of the observation period, a gross necropsy was conducted and localized tolerance at the injection site was analyzed.</p><p><strong>Results: </strong>No mortality was observed in the NPP test material and control groups. Moreover, no test substance-related effects were observed on clinical signs, body weight, necropsy findings, and localized tolerance at the injection site.</p><p><strong>Conclusion: </strong>The approximate lethal dose of the NPP agent is greater than 1.0 mL/animal under the conditions used in this study. Additional toxicity evaluations and clinical studies are needed to confirm the safety of NPP use in clinical practice.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 1","pages":"86-93"},"PeriodicalIF":1.4,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6a/54/jop-26-1-86.PMC10063425.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9240913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical, Antidiabetic, Antioxidant, Antibacterial, Acute and Sub-Chronic Toxicity of Moroccan <i>Arbutus unedo</i> Leaves.","authors":"Latifa Doudach,&nbsp;Hanae Naceiri Mrabti,&nbsp;Samiah Hamad Al-Mijalli,&nbsp;Mohamed Reda Kachmar,&nbsp;Kaoutar Benrahou,&nbsp;Hamza Assaggaf,&nbsp;Ahmed Qasem,&nbsp;Emad Mohamed Abdallah,&nbsp;Bodour Saeed Rajab,&nbsp;Khouloud Harraqui,&nbsp;Mouna Mekkaoui,&nbsp;Abdelhakim Bouyahya,&nbsp;Moulay El Abbes Faouzi","doi":"10.3831/KPI.2023.26.1.27","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.1.27","url":null,"abstract":"<p><strong>Objectives: </strong>Moroccan <i>Arbutus unedo</i> is an essential medicinal plant; however, little is known about the biological properties of its leaves mentioned in Moroccan traditional medicine.</p><p><strong>Methods: </strong>Various standard experiments were performed to evaluate the phytochemical, antidiabetic, antioxidant, antibacterial, and acute and sub-chronic toxicity characteristics of <i>A. unedo</i> leaves.</p><p><strong>Results: </strong>Phytochemical screening led to the identification of several phytochemical classes, including tannins, flavonoids, terpenoids, and anthraquinones, with high concentrations of polyphenols (31.83 ± 0.29 mg GAEs/g extract) and flavonoids (16.66 ± 1.47 mg REs/g extract). Further, the mineral analysis revealed high levels of calcium and potassium. <i>A. unedo</i> extract demonstrated significant antioxidant and anti-diabetic activities by inhibiting α-amylase (1.350 ± 0.32 g/mL) and α-glucosidase (0.099 ± 1.21 g/mL) compared to the reference drug Acarbose. Also, the methanolic extract of the plant exhibited significantly higher antibacterial activity than the aqueous extract. Precisely, three of the four examined bacterial strains exhibited substantial susceptibility to the methanolic extract . Minimum bactericidal concentration (MBC)/minimum inhibitory concentration (MIC) values indicated that <i>A. unedo</i> harbor abundant bactericidal compounds. For toxicological studies, mice were administered with <i>A. unedo</i> aqueous extract at single doses of 2,000 and 5,000 mg/kg. They did not exhibit significant abnormal behavior, toxic symptoms, or death during the 14-day acute toxicity test and the 90-day sub-chronic toxicity test periods. The general behavior, body weight, and hematological and biochemical status of the rats were assessed, revealing no toxicological symptoms or clinically significant changes in biological markers observed in the mice models, except hypoglycemia, after 90 days of daily dose administration.</p><p><strong>Conclusion: </strong>The study highlighted several biological advantages of <i>A. unedo</i> leaves without toxic effects in short-term application. Our findings suggest that conducting more comprehensive and extensive <i>in vivo</i> investigations is of utmost importance to identify molecules that can be formulated into pharmaceuticals in the future.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 1","pages":"27-37"},"PeriodicalIF":1.4,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/30/12/jop-26-1-27.PMC10063422.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9234409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Effect of <i>Allium ampeloprasum</i> and <i>Allium porrum</i> Extracts on <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>.","authors":"Shahin Gavanji,&nbsp;Azizollah Bakhtari,&nbsp;Hojjat Baghshahi,&nbsp;Zahra Hamami Chamgordani,&nbsp;Akram Sadeghi","doi":"10.3831/KPI.2023.26.1.53","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.1.53","url":null,"abstract":"<p><strong>Objectives: </strong>The most prominent microorganisms that cause hospital infections and acquire antibiotic resistance are <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>. The present study aimed to compare the phenolic and flavonoid compounds of various <i>Allium ampeloprasum</i> and <i>Allium porrum</i> extracts and evaluate the antibacterial effects of these extracts against these two microorganisms.</p><p><strong>Methods: </strong>The total phenolic and flavonoid contents of the acetone, methanol, aqueous, and hexane leeks extracts from <i>A. ampeloprasum</i> and <i>A. porrum</i> were measured. The antibacterial activity of these extracts against <i>S. aureus</i> and <i>P. aeruginosa</i> was tested using the disk diffusion method for 24, 48, and 72 hours. Further, the minimum inhibitory concentrations and the minimum bactericidal concentrations of these extracts for these two bacteria were evaluated and compared with those of common antibiotics.</p><p><strong>Results: </strong>The aqueous extracts showed the highest phenolic and flavonoid contents and at concentrations of 35 and 40 mg per disk, showed the most antibacterial activity against <i>S. aureus</i> and <i>P. aeruginosa</i>; <i>P. aeruginosa</i> showed more sensitivity to the aqueous extracts than <i>S. aureus</i>.</p><p><strong>Conclusion: </strong>Aqueous <i>A. ampeloprasum</i> and <i>A. porrum</i> extracts may prevent the growth of hospital pathogens, especially <i>P. aeruginosa</i>; our findings will aid the discovery of new antimicrobial substances against antibiotic-resistant bacteria.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 1","pages":"53-59"},"PeriodicalIF":1.4,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f5/8e/jop-26-1-53.PMC10063418.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9241553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Brief Review on <i>Murraya paniculata</i> (Orange Jasmine): pharmacognosy, phytochemistry and ethanomedicinal uses.","authors":"Deepa Joshi,&nbsp;Kashmira J Gohil","doi":"10.3831/KPI.2023.26.1.10","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.1.10","url":null,"abstract":"<p><strong>Objectives: </strong><i>Murraya paniculata</i> (family-Rutaceae), popularly known as orange jasmine, is the most important evergreen plant. The Rutaceae family is economically significant due to its diverse edible fruits and essential oils.</p><p><strong>Methods: </strong><i>Murraya paniculata</i> extracts (MPE) of leaf have been shown to include phenolic compounds, highly oxygenated flavonoids, flavanones, sesquiterpenoids, polymethoxy glycosides, and coumarins. Cyclocitral, methyl salicylate, trans-nerolidol, cubenol, isogermacrene, -cadinol, and cubeb-11-ene are all abundant in MPE. The usages of various parts of this plant, such as bark, leaves and flower, as a remedy for a variety of ailments as widely recorded in the traditional literature. The plant has anti-diabetic, anti-obesity, antibacterial, anti-implantation, anti-oxidative, cytotoxic, anti-diarrheal, antidepressant and anti-anxiety properties and many others.</p><p><strong>Results: </strong>The goal of the review is to reignite interest in this potential plant, encouraging researchers to continue their research in order to uncover novel therapeutic compounds for the treatment and management of a range of infections. The current review provided a comprehensive overview of this traditional unique plant.</p><p><strong>Conclusion: </strong>The review paves a way for exploring its active chemical elements with substantial pharmacological values further for potential benefits of mankind.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 1","pages":"10-17"},"PeriodicalIF":1.4,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0f/3b/jop-26-1-10.PMC10063420.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9246841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Antimicrobial Activity of Extracts from <i>Quercus infectoria</i> Galls and <i>Scrophularia striata</i> Aerial Parts for an Anticariogenic Herbal Mouthwash.","authors":"Pooya Falakdin,&nbsp;Dara Dastan,&nbsp;Shabnam Pourmoslemi","doi":"10.3831/KPI.2023.26.1.44","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.1.44","url":null,"abstract":"<p><strong>Objectives: </strong>Dental caries is one of the most prevalent human diseases worldwide. The disease initiates with bacterial adherence to the tooth surface followed by the formation of dental plaques. Mutans streptococci and <i>Candida albicans</i> are principal oral microorganisms involved in the initiation and development of dental caries. Phytochemicals have been shown to possess promising antimicrobial properties against a wide range of microorganisms and can be used for the prevention and treatment of dental caries. Herein, we reviewed literature on plants that are traditionally used for their antimicrobial properties or possess promising anticariogenic activity. We selected aerial parts of <i>Scrophularia striata</i> (<i>S. striata</i>) and galls of <i>Quercus infectoria</i> (<i>Q. infectoria</i>) and investigated their antimicrobial activity against cariogenic microorganisms.</p><p><strong>Methods: </strong>Water soluble fractions were obtained from hydroalcoholic extracts of <i>S</i>. <i>striata</i> and <i>Q</i>. <i>infectoria</i> and their antimicrobial activity against <i>Streptococcus mutans</i> (<i>S. mutans</i>), <i>Streptococcus sobrinus</i> (<i>S. sobrinus</i>), and <i>Candida albicans</i> (<i>C. albicans</i>) was evaluated separately and in combination. The extracts were then used for preparing an herbal mouthwash whose stability and tannic acid content were evaluated over 60 days.</p><p><strong>Results: </strong><i>Q</i>. <i>infectoria</i> gall extract possesses efficient antimicrobial activity that was synergistically enhanced in the presence of <i>S</i>. <i>striata</i> extract. Mouthwash prepared using these extracts showed desirable organoleptic characteristics, antimicrobial activity, and stability.</p><p><strong>Conclusion: </strong>Extracts of <i>S</i>. <i>striata</i> and <i>Q</i>. <i>infectoria</i> galls can be used together for preparing dental products with effective anticariogenic properties. Our study highlights the importance of extensive pharmacological investigations when using herbal products alone or in combination with other chemical substances.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 1","pages":"44-52"},"PeriodicalIF":1.4,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/58/fb/jop-26-1-44.PMC10063423.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9594967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Auraptene against Free Radical-Induced Erythrocytes Damage.","authors":"Khadijeh Jamialahmadi,&nbsp;Amir Hossein Amiri,&nbsp;Fatemeh Zahedipour,&nbsp;Fahimeh Faraji,&nbsp;Gholamreza Karimi","doi":"10.3831/KPI.2022.25.4.344","DOIUrl":"https://doi.org/10.3831/KPI.2022.25.4.344","url":null,"abstract":"<p><strong>Objectives: </strong>Auraptene is the most abundant natural prenyloxycoumarin. Recent studies have shown that it has multiple biological and therapeutic properties, including antioxidant properties. Erythrocytes are constantly subjected to oxidative damage that can affect proteins and lipids within the erythrocyte membrane and lead to some hemoglobinopathies. Due to the lack of sufficient information about the antioxidant effects of auraptene on erythrocytes, this study intended to evaluate the potential of this compound in protecting radical-induced erythrocytes damages.</p><p><strong>Methods: </strong>The antioxidant activity of auraptene was measured based on DPPH and FRAP assays. Notably, oxidative hemolysis of human erythrocytes was used as a model to study the ability of auraptene to protect biological membranes from free radical-induced damage. Also, the effects of auraptene in different concentrations (25-400 µM) on AAPH-induced lipid/protein peroxidation, glutathione (GSH) content and morphological changes of erythrocytes were determined.</p><p><strong>Results: </strong>Oxidative hemolysis and lipid/protein peroxidation of erythrocytes were significantly suppressed by auraptene in a time and concentration-dependent manner. Auraptene prevented the depletion of the cytosolic antioxidant GSH in erythrocytes. Furthermore, it inhibited lipid and protein peroxidation in a time and concentration-dependent manner. Likewise, FESEM results demonstrated that auraptene reduced AAPH-induced morphological changes in erythrocytes.</p><p><strong>Conclusion: </strong>Auraptene efficiently protects human erythrocytes against free radicals. Therefore, it can be a potent candidate for treating oxidative stress-related diseases.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 4","pages":"344-353"},"PeriodicalIF":1.4,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/14/df/jop-25-4-344.PMC9806157.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9084276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Cytotoxic Responses Induced by Treatment of A549 Human Lung Cancer Cells with Sweet Bee Venom in a Dose-Dependent Manner.","authors":"Yu-Na Hwang,&nbsp;In-Seo Kwon,&nbsp;Han-Heom Na,&nbsp;Jin-Sung Park,&nbsp;Keun-Cheol Kim","doi":"10.3831/KPI.2022.25.4.390","DOIUrl":"https://doi.org/10.3831/KPI.2022.25.4.390","url":null,"abstract":"<p><strong>Objectives: </strong>Sweet bee venom (sBV) is purified from <i>Apis mellifera</i>, containing a high level of melittin-its main component. It has been used as a therapeutic agent for pain relief and anti-inflammation, as well as for treating neuronal abnormalities. Recently, there have been studies on the therapeutic application of sBV for anticancer treatment. In the present study, we investigated the pharmacological effect of sBV treatment in A549 human lung cancer cells.</p><p><strong>Methods: </strong>We used microscopic analysis to observe the morphological changes in A549 cells after sBV treatment. The MTT assay was used to examine the cytotoxic effect after dose-dependent sBV treatment. Molecular changes in sBV were evaluated by the expression of apoptosis marker proteins using western blot analysis.</p><p><strong>Results: </strong>Microscopic analysis suggested that the growth inhibitory effect occurred in a dose-dependent manner; however, cell lysis occurred at a concentration over 20 μg/mL of sBV. The MTT assay indicated that sBV treatment exhibited a growth inhibitory effect at a concentration over 5 μg/mL. On fluorescence activated cell sorting analysis, G0 dead cells were observed after G1 arrest at treatment concentrations up to 10 μg/mL. However, rapid cell rupture was observed at a concentration of 20 μg/mL. Western blot analysis demonstrated that sBV treatment modulated the expression of multiple cell death-related proteins, including cleaved-PARP, cleaved-caspase 9, p53, Bcl2, and Bax.</p><p><strong>Conclusion: </strong>sBV induced cell death in A549 human lung cancer cells at a pharmacological concentration, albeit causing hemolytic cell death at a high concentration.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"25 4","pages":"390-395"},"PeriodicalIF":1.4,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f2/3a/jop-25-4-390.PMC9806155.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9084279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}