Adansonia digitata L. Stem Bark Attenuates Epileptic Seizure, Depression, and Neurodegeneration by Mediating GABA and Glutamate in Pentylenetetrazol-Kindled Rats.

IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Pharmacopuncture Pub Date : 2023-12-31 DOI:10.3831/KPI.2023.26.4.327

Adamu Muhammad, Luteino Lorna Hamman, Samaila Musa Chiroma, Martha Orendu Oche Attah, Nathan Isaac Dibal

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引用次数: 0

Abstract

Objectives: Epilepsy is a neurological condition characterized by repeated seizures attributable to synchronous neuronal activity in the brain. The study evaluated the effect of acetone extract of Adansonia digitata stem bark (ASBE) on seizure score, cognition, depression, and neurodegeneration as well as the level of Gamma-Aminobutyrate acid (GABA) and glutamate in Pentylenetetrazol-kindled rats.

Methods: Thirty-five rats were assigned into five groups (n = 7). Groups 1-2 received normal saline and 35 mg/kg PTZ every other day. Groups 3-4 received 125 mg/kg and 250 mg/kg ASBE orally while group 5 received 5 mg/kg diazepam daily for twenty-six days. Group 3-5 received PTZ every other day, 30 mins after ASBE and diazepam.

Results: The results showed that Pentylenetetrazol (PTZ) induces seizure, reduces mobility time in force swim test and decreases the normal cell number in the brain. It also significantly decreases (p < 0.05) catalase, superoxide dismutase and reduced glutathione activities compared to the ASBE pre-treated rats. Pre-treatment with ASBE reportedly decreases seizure activities significantly (p < 0.05) and increases mobility time in the force swim test. ASBE also significantly elevate (p < 0.05) the normal cell number in the hippocampus, temporal lobe, and dentate gyrus.

Conclusion: ASBE reduced seizure activity and prevented depression in PTZ-treated rats. It also prevented neurodegeneration by regulating glutamate and GABA levels in the brain as well as preventing lipid peroxidation.

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Adansonia digitata L. 茎皮通过调节 GABA 和谷氨酸来减轻戊四唑诱导的大鼠的癫痫发作、抑郁和神经退行性变。

目的：癫痫是一种神经系统疾病，其特点是大脑神经元同步活动导致癫痫反复发作。本研究评估了 Adansonia digitata 茎皮丙酮提取物（ASBE）对戊四唑诱导的大鼠癫痫发作评分、认知能力、抑郁、神经变性以及γ-氨基丁酸（GABA）和谷氨酸水平的影响：将 35 只大鼠分为 5 组（n = 7）。第 1-2 组接受生理盐水和 35 毫克/千克 PTZ，隔日一次。第 3-4 组口服 125 毫克/千克和 250 毫克/千克 ASBE，第 5 组每天口服 5 毫克/千克地西泮，连续 26 天。第 3-5 组每隔一天服用一次 PTZ，在服用 ASBE 和地西泮后 30 分钟服用：结果表明，戊四唑（PTZ）会诱发癫痫发作，缩短用力游泳测试的移动时间，并减少大脑中正常细胞的数量。与预先用 ASBE 处理的大鼠相比，PTZ 还会明显降低过氧化氢酶、超氧化物歧化酶和还原型谷胱甘肽的活性（p < 0.05）。据报道，用 ASBE 进行预处理后，癫痫发作活动明显减少（p < 0.05），用力游泳试验中的活动时间增加。ASBE还能明显增加海马、颞叶和齿状回的正常细胞数量（p < 0.05）：结论：ASBE可减少PTZ治疗大鼠的癫痫发作活动，防止抑郁。结论：ASBE 可减轻 PTZ 治疗大鼠的癫痫发作活动，防止抑郁，还可通过调节脑内谷氨酸和 GABA 水平以及防止脂质过氧化防止神经变性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Pharmacopuncture INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

2.10

自引率

7.10%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Pharmacopuncture covers a wide range of basic and clinical science research relevant to all aspects of the biotechnology of integrated approaches using both pharmacology and acupuncture therapeutics, including research involving pharmacology, acupuncture studies and pharmacopuncture studies. The subjects are mainly divided into three categories: pharmacology (applied phytomedicine, plant sciences, pharmacology, toxicology, medicinal plants, traditional medicines, herbal medicine, Sasang constitutional medicine, herbal formulae, foods, agricultural technologies, naturopathy, etc.), acupuncture (acupressure, electroacupuncture, laser acupuncture, moxibustion, cupping, etc.), and pharmacopuncture (aqua-acupuncture, meridian pharmacopuncture, eight-principles pharmacopuncture, animal-based pharmacopuncture, mountain ginseng pharmacopuncture, bee venom therapy, needle embedding therapy, implant therapy, etc.). Other categories include chuna treatment, veterinary acupuncture and related animal studies, alternative medicines for treating cancer and cancer-related symptoms, etc. Broader topical coverage on the effects of acupuncture, the medical plants used in traditional and alternative medicine, pharmacological action and other related modalities, such as anthroposophy, homeopathy, ayurveda, bioelectromagnetic therapy, chiropractic, neural therapy and meditation, can be considered to be within the journal’s scope if based on acupoints and meridians. Submissions of original articles, review articles, systematic reviews, case reports, brief reports, opinions, commentaries, medical lectures, letters to the editor, photo-essays, technical notes, and book reviews are encouraged. Providing free access to the full text of all current and archived articles on its website (www.journal.ac), also searchable through a Google Scholar search.