Journal of periodontology最新文献

{"title":"Effectiveness of nonsurgical re‐instrumentation of residual pockets as step 3 of periodontal therapy: A field study","authors":"Anna Liss, Kajsa H. Abrahamsson, Maria Welander, Cristiano Tomasi","doi":"10.1002/jper.24-0532","DOIUrl":"https://doi.org/10.1002/jper.24-0532","url":null,"abstract":"BackgroundThe study aims to analyze the effectiveness of nonsurgical re‐instrumentation of residual pockets as step 3 of periodontal therapy and the stability of treatment outcomes at 18 months.MethodsThe study sample consisted of 489 patients diagnosed and treated nonsurgically for periodontitis. After a 6‐month evaluation, residual pockets (≥5 mm) were re‐instrumented. A final examination was performed at 18 months. Participants were categorized by treatment outcome at 6 months: (A) No residual pockets ≥5 mm, (B) Residual pockets 5–6 mm, or (C) Residual pockets ≥7 mm. The primary outcome was pocket closure (≤4 mm). Logistic regression models were built to evaluate the effectiveness of re‐instrumentation and stability of initial outcomes at 18 months.ResultsRe‐instrumentation of pockets 5 to 6 mm resulted in pocket closure of around 39% in groups B and C. The corresponding result at sites with deeper residual pocketing (probing pocket depth [PPD] ≥7 mm) was 28%. Combining hand and ultrasonic instrumentation was more effective than each alone. Healed sites (6 months) were more likely to remain closed in group A (80%) than in groups B (50%) and C (40%). The logistic regression revealed that the stability of outcomes of step 1 and step 2 therapy was influenced by PPD at baseline, tobacco smoking, age, and type of tooth.ConclusionsAt step 3, nonsurgical re‐instrumentation can effectively improve periodontal health conditions. Treatment decisions after nonsurgical therapy should be based on residual probing depth, overall healing response, and patient behavior. The stability of clinical results was influenced by disease severity and the effectiveness of step 2 therapy.Plain Language SummaryThis study examined the effectiveness of nonsurgical re‐treatment of residual pockets (periodontal pockets not healed) after initial therapy and the stability of treatment outcomes to 18 months. The study involved 489 patients with periodontitis (loss of tooth‐supporting structures) treated in general dental practice. Residual pockets were re‐treated after examination at 6 months. A final examination was performed at 18 months. Treatment of residual gingival pockets was more successful in moderately diseased pockets compared with the most severely diseased pockets. Clinical results achieved in patients with a mild form of periodontitis were better in terms of stability compared with patients suffering from moderate and severe periodontitis. The success of nonsurgical periodontal treatment was influenced by the severity of periodontitis at the study start, the type of tooth affected, the age of the patient, and whether the patient was a smoker or not.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"30 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric analysis of intraoral scans: A novel approach for detecting gingival inflammation","authors":"Mohamed Ahmed Hassan, Guilherme Castro Lima Silva do Amaral, Luciana Saraiva, Marinella Holzhausen, Fausto Medeiros Mendes, Claudio Mendes Pannuti, Bernal Stewart, Zilson M. Malheiros, Carlos Benítez, Laís Yumi Souza Nakao, Cristina Cunha Villar, Giuseppe Alexandre Romito","doi":"10.1002/jper.24-0389","DOIUrl":"https://doi.org/10.1002/jper.24-0389","url":null,"abstract":"BackgroundGingivitis, a widely prevalent oral health condition, affects up to 80% of the population. Traditional assessment methods for gingivitis rely heavily on subjective clinical evaluation. This study seeks to explore the efficacy of interpreting the color metrics from intraoral scans to objectively differentiate between healthy and inflamed gingiva.MethodsThis study used the percentage of bleeding on probing (BOP%) as the clinical reference standard. Intraoral scans, obtained before and after gingivitis treatment using a scanner, were analyzed through a custom MATLAB script to quantify HSV (hue, saturation, value) and CIELAB (Commission Internationale de l'Eclairage L*a*b*) color coordinates. The region of interest was a 2‐mm‐wide gingival strip along the buccal margin of the maxillary anterior teeth. Linear regression analysis was performed to evaluate the relationship between photometric outcomes and continuous, dichotomous, and categorical BOP data. Diagnostic accuracy was assessed using the area under the receiver operating characteristic (ROC) curve (AUC), as well as sensitivity and specificity measures.ResultsThe analysis included clinical and digital color data from 110 scans, adhering to the STROBE (Strengthening the Reporting of Observational Studies in Epidemiology) guidelines. The multilevel linear regression analysis underscored a significant correlation between the BOP% and digital color metrics, specifically the CIELAB a* (red‐green chroma), CIELAB b* (yellow‐blue chroma), and color saturation, with AUC performances of 70%, 79.5%, and 80.8%, respectively.ConclusionDigital color analysis of intraoral scans has demonstrated a range of performance from acceptable to excellent in distinguishing sites with BOP. This innovative approach presents a promising tool for dentists and researchers in the accurate diagnosis, screening, and management of gingivitis.Plain Language SummaryOur study focuses on finding a better way to detect gingivitis, a common gum disease affecting many people. Traditional methods rely on the dentist's visual inspection, which can be subjective. We explored the use of color measurements from digital intraoral scans to objectively identify healthy versus inflamed gums. We analyzed 110 scans from 55 participants, examining the color differences in the gums before and after treatment. By measuring specific color values, we achieved up to 80.8% accuracy in distinguishing between healthy and inflamed gums. This method could offer a more reliable tool for dentists and researchers to diagnose and manage gingivitis, leading to better oral health outcomes.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"37 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cotinine, trans-3'-hydroxycotinine, and nicotine metabolite ratio indicate association between smoking and tooth loss.","authors":"Yuwei Zhang, Yue Jia, Changjie Sun, Yifei Zhang, Yuxin Liao, Huizhe Liu, Rui Zou, Lin Niu, Shaojie Dong","doi":"10.1002/JPER.24-0249","DOIUrl":"https://doi.org/10.1002/JPER.24-0249","url":null,"abstract":"<p><strong>Background: </strong>Previous research has indicated a potential connection between smoking and tooth loss, but it remains unclear how the metabolites of nicotine, cotinine (COT) and trans-3'-hydroxycotinine (HC), and the nicotine metabolite ratio (NMR) affect the occurrence and progress of tooth loss. In this study, we aimed to investigate the relationship between tooth loss and smoking metabolites, then verify how the systemic immunoinflammatory index (SII) or monocyte to high-density lipoprotein cholesterol ratio (MHR) levels mediate this process.</p><p><strong>Methods: </strong>The cross-sectional study data were collected from the National Health and Nutrition Examination Survey (NHANES). After screening and comparing the baseline data, regression models were utilized to evaluate the relationship between serum COT + HC level and tooth loss, as well as COT + HC and SII/MHR and tooth loss. Furthermore, bootstrapping was applied to test the mediation effect of SII and MHR in the association between COT + HC and tooth loss.</p><p><strong>Results: </strong>A total of 9036 subjects were included in the study, with 2795 individuals in the COT/HC- group and 6241 in the COT/HC+ group. In the models with covariates adjusted, COT + HC level was found to be positively correlated with tooth loss (Model 3: odds ratio [OR] = 1.001, 95% CI = 1.001-1.001), especially in the fourth quartile (Q4) of COT + HC level (Model 3: OR = 2.647, 95% CI = 2.645-2.649) as compared to Q1. Furthermore, NMR was found to be negatively associated with tooth loss after adjustments (Model 3: OR = 0.803, 95% CI = 0.802-0.803). For mediation analysis, COT + HC level was significantly correlated with SII score, and SII was proved to be positively associated with tooth loss; meanwhile, COT + HC level was significantly correlated with MHR, and MHR was positively associated with tooth loss. SII and MHR played a partial mediating role in the association between COT + HC and tooth loss, and the indirect effect was 0.0001 (SII; 95% CI = 0.0000-0.0002) and 0.0002 (MHR; 95% CI = 0.0001-0.0004), respectively. The mediating effect of both SII and MHR was 0.0003 (95% CI = 0.0001-0.0004).</p><p><strong>Conclusions: </strong>This study revealed that the level of nicotine metabolites (COT + HC) was a risk factor, while higher NMR was a protective factor for tooth loss, and further demonstrated the partial mediating role of SII and MHR in the association between COT + HC and tooth loss.</p><p><strong>Plain language summary: </strong>Prior studies have suggested a possible link between smoking and tooth loss, but how certain nicotine byproducts and their ratios impact tooth loss is unclear. This research aimed to explore how these smoking metabolites influence tooth loss. Data from national health surveys were used for a cross-sectional study to assess the connection between cotinine levels in the blood and tooth loss, as well as to understand if immune and cholesterol markers mediate t","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Forum Poster Session and Competition: Finalists and Posters","authors":"","doi":"10.1002/jper.24-0266","DOIUrl":"https://doi.org/10.1002/jper.24-0266","url":null,"abstract":"","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"36 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of rheumatoid arthritis on peri-implant diseases: A longitudinal retrospective clinical and radiographic evaluation.","authors":"Hamzeh Almashni, Era Kakar, Paolo Nava, Hom-Lay Wang, Muhammad H A Saleh","doi":"10.1002/JPER.24-0376","DOIUrl":"https://doi.org/10.1002/JPER.24-0376","url":null,"abstract":"<p><strong>Background: </strong>To investigate the effect of rheumatoid arthritis (RA) on the incidence of peri-implantitis (PI) and peri-implant mucositis (PIM).</p><p><strong>Methods: </strong>Radiographic and clinical chart reviews were conducted to measure the probing depth (PD), bleeding on probing, and marginal bone loss (MBL) around the implants to diagnose peri-implant diseases based on the 2017 workshop classification. Values were recorded at the baseline (T0) to the last available chart and radiograph (T1). Maintenance compliance was evaluated. Cases were followed longitudinally to detect the incidence of PI and PIM. Various potential confounders were controlled, including the total radiographic follow-up time, chart-based follow-up time, number of maintenance visits, implant dimensions, history of periodontitis, bone graft, restoration angle, emergence, smoking, and diabetes mellitus. Chi-square and Mann-Whitney tests evaluated categorical and continuous differences. Generalized estimating equations with a Tweedie distribution were applied. Binary logistic regression ascertained the odds ratio for binary outcomes.</p><p><strong>Results: </strong>A total of 101 patients (50 RA and 51 non-RA) with 124 implants were investigated. The mean follow-up period for the implants was 5.38 ± 2.22 years. Implant survival rate was high at 96%. The RA group demonstrated a significantly higher PI (p = 0.024), while the non-RA group showed a significantly higher PIM (p < 0.001). No significant differences were observed between both groups in implant survival and MBL.</p><p><strong>Conclusion: </strong>Compared to the non-RA group, RA patients demonstrated a similar incidence of MBL and implant survival rates and a significantly lower incidence of PIM; however, there was a significantly higher incidence of PI.</p><p><strong>Plain language summary: </strong>In this study, we investigated the condition of dental implants in 50 patients with rheumatoid arthritis (RA) compared to 51 healthy controls over 5 years. Assessments from initial treatment to the last follow-up visit included reviewing patient records and radiographs for signs of bleeding, probing depth, and bone loss. These measures helped diagnose peri-implantitis (PI) and peri-implant mucositis (PIM) based on the 2017 periodontal disease classification. The findings revealed a high implant survival rate in both groups (96%) with no significant difference in bone loss. However, the RA group showed a significantly higher incidence of PI than the healthy group that demonstrated PIM.</p>","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Forum Poster Session and Competition: Finalists and Posters.","authors":"","doi":"10.1002/JPER.24-0369","DOIUrl":"https://doi.org/10.1002/JPER.24-0369","url":null,"abstract":"","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Excess cement and peri-implant disease: A cross-sectional clinical endoscopic study.","authors":"Marco Montevecchi, Leoluca Valeriani, Maria Francesca Salvadori, Martina Stefanini, Giovanni Zucchelli","doi":"10.1002/JPER.24-0510","DOIUrl":"https://doi.org/10.1002/JPER.24-0510","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Crown cementation is a common technique for implant-supported prosthodontics. However, for possible slipping of the cement below the mucosal margin, its thorough removal poses some issues. The objective of this study was to evaluate the presence of submucosal cement residues in patients with peri-implant disease by endoscopic visualization and to investigate the potential correlation between the pathological scenario and the spatial position of cement residues.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The study sample comprised 46 consecutive patients exhibiting clinical and radiographic signs of peri-implant disease around cement-retained crowns. When at first visit bleeding on probing was the only pathological sign, the area was debrided and then treated with antiseptic agents for 30 days. Only those patients for whom bleeding was still found at re-evaluation were included in the study. All implants were therefore endoscopically evaluated to identify possible submucosal cement residues. For all implants showing residual cement, the spatial position of the residual cement was recorded with respect to predefined points.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Cement residues were detected in 80.4% of the patients and were predominantly located in the vestibular and lingual areas (88%). Analysis of the distances between the apical portion of the residues and anatomical landmarks revealed significant differences between mucositis and peri-implantitis patients (p = 0.001). In cases of peri-implantitis, the cement residue was more apically located than in mucositis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;The presence of cement residue was associated with peri-implant disorders in the majority of patients. A potential correlation between the position of residues and the peri-implant disease scenario is here suggested.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Plain language summary: &lt;/strong&gt;A common method for fixing the crown on the dental implant is the use of specific cements. However, these products can slip below the gum line resulting in difficulties in their removal, which could cause peri-implant disease. This study aimed to evaluate the presence and location of residual cement hidden under the gums in patients with peri-implant disease, characterized by symptoms such as bleeding gums and, in some cases, bone loss around the dental implant. Using a small fiber-optic camera (endoscope), 46 patients were examined for any cement residue under the gums. The study showed that more than 80% of patients had cement residues, especially in the areas facing the cheeks and tongue. It was also found that in patients with a more severe form of inflammation (peri-implantitis), the cement was placed deeper than in those with a milder form (mucositis). This confirms that residual cement is common in patients with peri-implant disease, but also suggests that its location could influence the severity of the pathological manifestation. This finding highlights the importance of ","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Forum Poster Session and Competition: Winners.","authors":"","doi":"10.1002/JPER.24-0373","DOIUrl":"https://doi.org/10.1002/JPER.24-0373","url":null,"abstract":"","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mediating role of immune cells in association between volatile organic compounds and periodontitis: NHANES 2011–2014","authors":"Wenxiu Jiang, Wei Wu, Kejia Zhang, Luwei Liu, Bin Yan","doi":"10.1002/jper.24-0150","DOIUrl":"https://doi.org/10.1002/jper.24-0150","url":null,"abstract":"BackgroundThe relationship between humans and volatile organic compounds (VOCs) is a persistent concern due to their widespread sources and high evaporation rates. However, there is currently limited direct evidence linking VOC exposure to the development of periodontitis.MethodsThis cross‐sectional study analyzed 1525 participants and 21 urinary VOCs in the National Health and Nutrition Examination Survey (NHANES) from 2011 to 2014, aiming to investigate the relationship between periodontitis risk, assessed by attachment loss (AL) and probing depth (PD) and individual VOCs using logistic regression, quantile regression, and subgroup analysis. Weighted quantile sum analysis (WQS) and subgroup analysis were utilized to evaluate whether VOC mixtures were associated with periodontitis risk. Multiple linear regression models were used to examine the association between VOC co‐exposure and peripheral immune cell counts. A mediation analysis was performed to evaluate whether peripheral immune cells are involved in the effect of VOC co‐exposure on periodontitis prevalence.ResultsUrinary levels of 2‐aminothiazoline‐4‐carboxylic acid, mandelic acid, and &lt;jats:italic&gt;N&lt;/jats:italic&gt;‐acetyl‐S‐(4‐hydroxy‐2‐butenyl)‐L‐cysteine were positively associated with the risk of periodontitis after adjusting for all covariates. The WQS models demonstrated a positive correlation between the mixture of VOCs and the risk of periodontitis, wherein 2‐aminothiazoline‐4‐carboxylic acid emerged as the most important contributor. The mediation analysis suggested that monocytes may play a role in the observed association between VOC co‐exposure and the prevalence of periodontitis.ConclusionsExposure to VOCs is associated with a greater prevalence of periodontitis. Monocytes' mediating role plays a crucial function in the association between the risk of periodontitis and co‐exposure to VOCs.Plain language summaryVolatile organic compounds (VOCs) are chemicals that evaporate quickly and are found all around us—from paints to cleaning products. Understanding how these compounds affect our health is crucial, especially regarding conditions like periodontitis, a common oral chronic inflammatory disease. In our study, we looked at urine samples from 1525 people who participated in a national health survey between 2011 and 2014 to find out if there is an association between VOC exposure and the risk of developing periodontitis. We found that certain chemicals in the urine, which show VOC exposure, were indeed associated with a greater risk of the disease. We further investigated the collective impact of these VOCs on the risk of periodontitis, revealing that certain chemicals exert a more significant influence than their counterparts. Additionally, our research hints at a potential role for monocytes in the interplay between VOCs and the risk of periodontitis. Our data suggest that exposure to VOCs could be associated with a greater likelihood of periodontitis, with monocytes potentia","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"48 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of fibulin‐3 ameliorates periodontal inflammation through reducing M1 macrophage polarization via EGFR/PI3K/AKT pathway","authors":"Hailin Mu, Beining Yang, Yan Wang, Shuo Wang, Wenqian Yu, Meie Jia, Wei Dong, Xinyi Wang, Xiaoxiao Xu, Zhipeng Dong, Baochen Yang, Xuemei Li, Jiawei Wang","doi":"10.1002/jper.24-0405","DOIUrl":"https://doi.org/10.1002/jper.24-0405","url":null,"abstract":"BackgroundThis study aimed to evaluate the role of fibulin‐3 (FBLN3) in macrophage polarization, its mechanism, and its effect on periodontitis.MethodsWe conducted studies on periodontitis using both clinical samples and ligature‐induced mouse periodontitis model. The inflammatory state was assessed using microcomputed tomography, hematoxylin and eosin staining, immunohistochemical staining, and immunofluorescence staining. In vitro, bone marrow‐derived macrophages, and RAW 264.7 macrophages were treated with lipopolysaccharide (LPS) and interleukin (IL)‐4 to induce polarization. The role of FBLN3 in macrophage polarization was investigated using overexpression plasmids or siRNAs. Furthermore, local injection of adeno‐associated virus was employed to suppress FBLN3 expression in periodontal tissues.ResultsFBLN3 levels were greater in periodontitis tissues. FBLN3 promoted M1 polarization and suppressed M2 polarization in macrophages. The overexpression of FBLN3 promoted M1 polarization via the EGFR/PI3K/AKT signaling pathway, an effect that the epidermal growth factor receptor (EGFR) inhibitor PD153035 reversed. Suppressing FBLN3 expression improved periodontal inflammation and reduced alveolar bone loss in periodontitis.ConclusionsFBLN3 suppression can mitigate periodontitis by decreasing the M1 macrophage ratio. FBLN3 regulates M1 macrophage polarization through the EGFR/PI3K/AKT signaling pathway.Plain language summaryDisruption in the collaboration between extracellular matrix (ECM) and immune system is a significant pathology in periodontitis. Macrophages are a crucial part of the immune system and have unique functions, such as polarization. Fibulin‐3, an ECM protein, may play a vital role in this dynamic interplay. Fibulin‐3 expression is elevated in periodontitis and is closely related to immune cell function. Inhibiting fibulin‐3 can alleviate periodontitis by reducing infiltration of immune cells and M1 macrophage ratio. Furthermore, fibulin‐3 promoted macrophage M1 polarization by activating the PI3K/AKT signaling pathway through EGFR binding. Our findings offer a clinically relevant rationale for immune response modulation through fibulin‐3.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"6 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}