Journal of periodontology最新文献

{"title":"Arg‐gingipain, myeloperoxidase, and anti‐CarP across the rheumatoid arthritis spectrum","authors":"Maliha Shahbaz, Anis Rageh Al‐Maleki, Chia Wei Cheah, Jazli Aziz, Karuthan Chinna, Peter Mark Bartold, Rathna Devi Vaithilingam","doi":"10.1002/jper.70009","DOIUrl":"https://doi.org/10.1002/jper.70009","url":null,"abstract":"BackgroundThe association between periodontitis (PD) and rheumatoid arthritis (RA) has been linked to autoantibodies. In recent years, Arg‐gingipain (Rgp) triggered myeloperoxidase (MPO) release, which mediates protein carbamylation to form anti‐carbamylated proteins (anti‐CarP), perpetuating RA progression has gained interest. This study assessed the association between Rgp with MPO and anti‐CarP in pre‐clinical RA (preRA), early RA (eRA), and established RA (RA) participants with PD.MethodsA total of 108 participants were categorized into preRA, eRA, RA, and nonRA controls with and without PD. Periodontal and rheumatological parameters were assessed. <jats:italic>Rgp‐B</jats:italic> gene expression from subgingival plaque and MPO and anti‐CarP in saliva and serum were assessed. Data were analyzed with SPSS Version 26.Results<jats:italic>Rgp‐B</jats:italic> gene expressions were similar across PD groups. In eRA‐PD, serum and saliva MPO and saliva anti‐CarP levels were highest; strong correlations were present between <jats:italic>rgp‐B</jats:italic> with clinical attachment loss (CAL) (<jats:italic>r</jats:italic> = 0.783), salivary MPO with visible plaque index (VPI) (<jats:italic>r</jats:italic> = 0.667), and gingival bleeding index (GBI) (<jats:italic>r</jats:italic> = 0.767), and salivary anti‐CarP with CAL (<jats:italic>r</jats:italic> = 0.667) and GBI (<jats:italic>r</jats:italic> = 0.850). Strong positive correlations were detected between salivary MPO and anti‐CarP in preRA‐PD (<jats:italic>r</jats:italic> = 0.903), eRA‐PD (<jats:italic>r </jats:italic>= 0.783), RA‐PD (<jats:italic>r</jats:italic> = 0.726), and nonRA‐PD (<jats:italic>r </jats:italic>= 0.470).Conclusion<jats:italic>Rgp‐B</jats:italic> gene expression was associated with PD status. Periodontal inflammation, particularly in early RA, was linked to elevated MPO and anti‐CarP levels, suggesting that local inflammation may amplify immune responses via MPO‐mediated carbamylation. These associations highlight the clinical relevance of periodontal assessment and management in RA patients and at‐risk individuals.Plain Language SummaryPeriodontitis (PD) and rheumatoid arthritis (RA) are chronic inflammatory diseases that may be linked through immune system activity. Studies suggest RA can begin developing before symptoms appear (preRA), with early immune changes occurring in the body. One possible trigger is the occurrence of myeloperoxidase (MPO)‐mediated carbamylation in the inflamed periodontium during the earliest phases of RA, which may precede the occurrence of autoantibodies, particularly anti‐carbamylated proteins (anti‐CarP). To date, these changes due to PD at different stages of RA have not been studied. This study investigated whether the <jats:italic>Porphyromonas gingivalis</jats:italic> enzyme, Arg‐gingipain (Rgp) is linked to MPO and anti‐CarP levels in individuals at different stages of RA. A total of 108 participants were recruited for this study, including preRA,","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"3 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145282794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary microbiota and clinical periodontal measures predicting cardiometabolic disease mortality: A nationwide survey","authors":"Hamdi S. Adam, Weihua Guan, Abigail J. Johnson, Sanaz Sedaghat, Charlene Goh, James S. Pankow, Ryan T. Demmer","doi":"10.1002/jper.11395","DOIUrl":"https://doi.org/10.1002/jper.11395","url":null,"abstract":"BackgroundAlthough periodontitis and oral microbiota are linked to cardiometabolic diseases (CMD), it is unclear if they similarly predict CMD mortality. We compared the predictive ability of salivary microbiota and periodontal disease measures for CMD mortality in the National Health and Nutrition Examination Survey (NHANES).MethodsWe included 5,037 adults aged ≥30 years (mean age [± standard deviation (SD)]: 48[± 14]; 50% male) from the 2009–2010 and 2011–2012 NHANES cycles. We used 16S rRNA sequencing data from saliva to operationalize microbial composition and diversity. We calculated the relative abundance log‐ratio of <jats:italic>Treponema</jats:italic> (linked with periodontal disease) to <jats:italic>Corynebacterium</jats:italic> (linked with periodontal health) to compute the Microbial Indicator of Periodontitis (MIP). Interproximal periodontal probing depth and clinical attachment loss were measured from periodontal examinations. Mortality was ascertained through 2019. Survey‐weighted Cox models regressed mortality rates on MIP, microbial diversity, and periodontal measures to estimate hazard ratios and 95% confidence intervals (HR [95% CI]).ResultsOver 8.8 median follow‐up years, there were 81 CMD and 267 all‐cause deaths. After multivariable adjustment, MIP was associated with increased CMD mortality risk (HR per 1‐SD: 2.10 [1.30–3.38]). Neither microbial diversity nor periodontitis measures were associated with CMD mortality. MIP was associated with periodontitis in multivariable modeling (risk ratio per 1‐SD: 1.29 [1.22–1.39]).ConclusionsIn a nationally representative cohort, greater baseline salivary <jats:italic>Treponema</jats:italic> to <jats:italic>Corynebacterium</jats:italic> ratio predicted increased CMD mortality risk, while microbial diversity metrics and periodontal parameters were not significantly associated with CMD mortality. Longitudinal studies that further contextualize the oral microbiota are warranted.Plain Language SummaryBacteria in the mouth that cause gum disease are linked to cardiometabolic diseases (e.g., diabetes, cardiovascular disease, kidney disease). However, it is not well understood if bacteria of the mouth can predict the risk of death due to cardiometabolic diseases. We used data from a nationwide survey of US adults to explore whether bacteria from saliva, collected from a single time point, are associated with the future risk of cardiometabolic disease death. We found that people with higher levels of gum disease bacteria were more likely to die from cardiometabolic diseases. Future studies are needed to better understand the role of gum disease bacteria in the development of cardiometabolic diseases and the risk of death.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"2 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145261021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peri-implant hypoxia as a potential barrier against ferroptotic mechanisms during peri-implant diseases: A cross-sectional study.","authors":"Büşra Yılmaz,Ali Gürkan,Beral Afacan,Harika Atmaca,Timur Köse,Gülnur Emingil","doi":"10.1002/jper.70001","DOIUrl":"https://doi.org/10.1002/jper.70001","url":null,"abstract":"BACKGROUNDHypoxia modulates inflammation and oxidative stress through hypoxia-inducible factor-1α (HIF-1α). Ferroptosis, an iron-dependent cell death process, is regulated by glutathione peroxidase-4 (GPX4) and involves lipid peroxidation markers like malondialdehyde (MDA). This study evaluates HIF-1α, GPX4, and MDA levels in peri-implant crevicular fluid (PICF) across peri-implant health, mucositis, and peri-implantitis.METHODSPICF samples were collected from 62 implants of 45 participants categorized into peri-implant health (PH), mucositis (PM), and peri-implantitis (PP) groups. Enzyme-linked immunosorbent assay (ELISA) quantified HIF-1α, GPX4, and MDA levels. Statistical analyses, including Kruskal-Wallis and Spearman correlation, assessed biomarker differences and associations.RESULTSTotal MDA levels were significantly lower in PM and PP compared to PH (p = 0.014, p = 0.046). GPX4 levels were elevated in PM compared to PH (p = 0.034) but lower in PP than in PM (p < 0.001). While HIF-1α levels did not significantly differ among groups, their concentrations were notably higher in PH. A significant positive correlation was found between the total amounts of HIF-1𝛼 and GPX4 (r = 0.460, p < 0.01).CONCLUSIONOur findings demonstrated increased GPX4 and decreased MDA levels in peri-implant mucositis and peri-implantitis compared to peri-implant health, suggesting that ferroptosis may be inhibited in the peri-implant environment. Moreover, the positive correlation between HIF-1α and GPX4 levels indicates a potential regulatory role of hypoxia in modulating ferroptotic pathways in peri-implant tissues.PLAIN LANGUAGE SUMMARYBone loss around dental implants can lead to serious problems, putting the success of these implants at risk. Understanding how these issues develop is key to preventing and treating them. In the human body, cells can sometimes get damaged and die due to iron and stress caused by harmful molecules. This process, called \"ferroptosis,\" has recently gained attention in oral health research. Our study looked at whether low oxygen levels around dental implants might affect this process. We collected fluid samples from 45 people and measured 3 important substances linked to cell health and stress. We found that in diseased areas, the levels of molecules showing cell damage were lower, while the levels of substances that help protect cells were higher. This suggests that low oxygen conditions might actually help protect tissues around dental implants by preventing cell damage. These insights could help guide better ways to prevent and treat problems related to dental implants in the future.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"6 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145241076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From primary to secondary care level: Assessing patient retention of periodontal staging and grading information.","authors":"Pasquale Santamaria,Rohan Mangalpara,Thamara Kumar,Tina Lipovec,Luigi Nibali","doi":"10.1002/jper.70008","DOIUrl":"https://doi.org/10.1002/jper.70008","url":null,"abstract":"BACKGROUNDAccurate communication of periodontal stage and grade by general dentists in primary care is critical for patient understanding and engagement, yet patient retention and self-reporting of this information upon referral to secondary care remains unclear.METHODSA total of 372 periodontal patients referred were informed about their diagnosis by their general dentists and then referred to secondary care level. Data were collected through an eight-item periodontal staging and grading (PSG) questionnaire, along with demographic, medical, and dental records. Periodontal diagnoses were classified by a specialist using the 2018 classification system. Associations between clinical diagnosis and patient perception were analyzed using Chi-square tests and Spearman's rank correlation.RESULTSWhile 46.9% of patients diagnosed with periodontitis reported to be informed of their condition, only 19.3% reported knowing their specific stage and grade. Among patients with advanced periodontitis (stage III/IV), self-reported severity often aligned with clinical staging. However, for early-stage disease (stage I/II), perceptions were less accurate, and only 30.2% of grade C patients recognized rapid progression. Significant correlations were found between patient-reported symptoms and clinician-assigned staging: tooth loss (ρ = 0.69, p < 0.0001), root exposure (ρ = 0.638, p < 0.0001), and tooth mobility (ρ = 0.55, p < 0.0001).CONCLUSIONMost patients referred to secondary care lacked information on their disease stage and grade. Severe stages and grades were better perceived by patients compared with mild forms of periodontitis. The PSG questionnaire offers a valuable tool for identifying knowledge gaps and guiding tailored discussions in clinical practice.PLAIN LANGUAGE SUMMARYOur study asked 372 people referred for gum disease to fill out a simple eight-question survey about how they see their own gum health and compared their answers to the detailed diagnosis made by periodontists. We found that fewer than one in five patients knew exactly how severe their disease was or how fast it was progressing. People with more advanced gum damage generally understood their condition, but those with mild disease often thought they were healthier than they really were. When patients reported losing teeth, seeing root surfaces, or noticing loose teeth, these experiences matched closely with the clinical measures of disease severity. These results show that many patients lack clear information about their gum health and that a brief questionnaire can help dentists pinpoint misunderstandings, improve patient education, and support better treatment decisions.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"100 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic consumption reduces alveolar bone loss and kidney damage in pregnant rats with experimental periodontitis.","authors":"Átila V V Nobre,Pedro H F Silva,Marina C G Del-Arco,Raquel F Gerlach,Rene S Oliezer,José E Tanus-Santos,Luciene C Figueiredo,Janaina S A M Evangelista,Flávia A C Furlaneto,Michel R Messora,Sérgio Luiz Salvador","doi":"10.1002/jper.11389","DOIUrl":"https://doi.org/10.1002/jper.11389","url":null,"abstract":"BACKGROUNDBifidobacterium animalis subsp. lactis HN019 (B. lactis HN019) is a probiotic bacterial strain with immunomodulatory properties. Its benefits have been observed in healthy and systemically compromised animals with periodontitis (PD). Our objective was to investigate the local and systemic effects of the systemic administration of B. lactis in pregnant rats with experimental periodontitis (EP).METHODSFor this, 48 pregnant rats were divided into four different groups (n = 12/group): Control (C), Probiotic (PROB), Periodontitis (PD), and Periodontitis + Probiotic (PD-PROB). EP was induced using a mixed model of cotton ligature placement and oral gavage of Porphyromonas gingivalis W83. On gestational day 19, the animals were euthanized for sample collection and analysis. Jaws, kidneys, and urine samples were collected for microtomographic, histological, histomorphometric, and biochemical analyses. The data were statistically analyzed (p &lt; 0.05) using nonparametric tests (Kruskal-Wallis) and analysis of variance (ANOVA) followed by Tukey and Dunn post hoc tests.RESULTSEP resulted in local and systemic damage, such as alveolar bone loss (ABL) and kidney damage, and the consumption of B. lactis HN019 resulted in improvements in these parameters. Regarding mandibular analyses, the PD-PROB group showed greater bone volume in the furcation region, a greater number and thickness of bone trabeculae, and less bone porosity and separation between trabeculae compared to the PD group (p &lt; 0.05). Regarding kidney analysis, the PD-PROB group showed lower glomerular and Bowman's capsule diameters and circumferences compared to the PD group (p &lt; 0.05).CONCLUSIONProbiotic consumption reduced damage in mandibular bone and kidney tissues in pregnant rats with EP.PLAIN LANGUAGE SUMMARYPeriodontitis (PD) is a destructive periodontal disease that can lead to tooth loss. The treatment for PD consists of scaling and root planing to remove calculus and plaque deposits; however, some systemic conditions make it difficult to control this disease. Probiotic bacteria have emerged as adjuvants in the treatment of infectious diseases, and their benefits have been demonstrated in the management of PD. The aims of the present study were to evaluate whether PD has a negative impact on pregnancy and whether the probiotic strain Bifidobacterium animalis subsp. lactis HN019 can reduce this impact. For this purpose, 48 pregnant rats were divided into four experimental groups (Control, Probiotic, PD, and PD + Probiotic), and samples of maternal and pup weights, as well as placentas, mandibles, urine, and kidneys, were collected and analyzed. We observed that PD negatively impacted pregnant rats, resulting in greater alveolar bone loss, increased expression of proteinuria and creatinine in urine, and kidney damage; systemic probiotic administration reduced these harmful effects. In addition, pups, as well as mothers supplemented with probiotic, exhibited higher weights and larger","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"18 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontitis and high phosphate intake alone or in combination adversely affect the kidney.","authors":"Ae Ri Kim,Min Seo Kim,Jiwon Seo,Eun-Jung Bak,Yun-Jung Yoo","doi":"10.1002/jper.70013","DOIUrl":"https://doi.org/10.1002/jper.70013","url":null,"abstract":"BACKGROUNDPeriodontitis and high phosphate (HP) intake can negatively affect the kidney in the presence of renal disease. This study aimed to evaluate the effects of periodontitis or HP intake, either alone or concurrently, on the periodontal tissue and the kidney under normal renal conditions.METHODSRats were divided into normal diet (C), HP diet (HP), tooth ligation and normal diet (P), and tooth ligation and HP diet (P+HP) groups. The mandibular first molars were ligated, and a 0.9% HP diet was provided for 12 weeks from the day of ligation. An additional group (P+HP+IFX) was administered infliximab (IFX), a tumor necrosis factor-alpha (TNF-α) inhibitor, weekly to evaluate the TNF-α inhibitory effect.RESULTSAlveolar bone loss and periodontal inflammation did not differ between the P and P+HP groups or between the C and HP groups. In the kidney, interstitial fibrosis and Col1a1 expression increased in the HP group, and ED1 expression increased in the P group, compared to the C group. Tubular basophilia, interstitial fibrosis, and the expression of Col1a1 and ED1 increased in the P+HP group compared to the other groups. The P+HP+IFX group showed a decrease in periodontal inflammation and these renal alterations compared to the P+HP group.CONCLUSIONRegardless of periodontitis, 0.9% HP intake did not affect periodontal tissue. Renal fibrosis and macrophage infiltration induced by HP intake and periodontitis, respectively, worsened when combined, indicating a synergistic adverse effect. These changes were reversed by IFX, suggesting that TNF-α inhibition may alleviate renal injury caused by periodontitis and HP intake.PLAIN LANGUAGE SUMMARYOur study examined the impact of periodontitis and a 0.9% high phosphate (HP) diet, individually and together, on periodontal tissue and kidney. We divided rats into 4 groups: normal diet, HP diet, periodontitis with a normal diet, and periodontitis with an HP diet, and assessed various periodontal and renal parameters. Although we did not observe any effects of HP intake on periodontal tissue, we found that HP intake worsened kidney health by increasing fibrosis, while periodontitis did so by increasing macrophage infiltration. Combined, these conditions worsen kidney health more than when each condition exists alone, causing more tubular basophilia, fibrosis, and macrophage infiltration. However, these negative effects were reversed with TNF-α inhibition. These findings indicate that the combination of periodontitis and HP intake exacerbates renal damage, which can be ameliorated by TNF-α inhibition.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"1 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating diagnostic accuracy and consistency in applying the 2017 periodontal classification among dental professionals.","authors":"Arwa Badahdah,Arwa Banjar,Amal Jamjoom,Mohammad Assaggaf,Lina Bahanan,Reem A Asiri,Reem Alsulami,Shatha Bamashmous,Brian L Mealey","doi":"10.1002/jper.70011","DOIUrl":"https://doi.org/10.1002/jper.70011","url":null,"abstract":"BACKGROUNDAccurate periodontal disease diagnosis is essential for optimal treatment planning and patient care. However, variability in applying the 2017 Periodontal Classification may affect diagnostic reliability and treatment outcomes. This study investigated diagnostic accuracy and consistency among periodontists, periodontal residents, and dental interns in Saudi Arabia.METHODSForty-four participants, including 13 periodontists, 14 periodontal residents, and 17 dental interns, independently classified 25 periodontitis cases. Agreement with a gold-standard diagnosis, established by expert periodontists using the 2017 Classification System, was assessed using descriptive statistics. Chi-square tests with Bonferroni-adjusted z-tests were used to compare agreement levels between rater groups. Inter-rater reliability was calculated using Fleiss' kappa, while Cohen's kappa was used to assess intra-rater reliability.RESULTSPeriodontists demonstrated the highest agreement with the gold standard (92.0%) for periodontitis diagnosis. Staging agreement was highest among residents (51.7%) and periodontists (49.1%). Grading accuracy was highest for grade C cases across all groups (60.7%). Underestimation was common across rater groups, with interns exhibiting the highest rates in staging (49.6%) and grading (58.3%). The second assessment demonstrated improved diagnostic accuracy across all groups. Inter-rater reliability ranged from fair to moderate across rater groups (κ = 0.22-0.60). Intra-rater reliability was highest among interns, indicating substantial agreement (κ = 0.63-0.75).CONCLUSIONFindings highlight considerable variability in the application of the 2017 Periodontal Classification among dental professionals, underscoring the role of clinical experience and training in influencing diagnostic accuracy. Structured calibration and targeted educational strategies are essential to improve diagnostic consistency, minimize misclassification, and support optimal patient care.PLAIN LANGUAGE SUMMARYUnderstanding gum disease correctly is important for providing patients with the right treatments. This study looked at how accurately different groups of dental professionals - specialists in gum disease (periodontists), dentists in training (residents), and recent dental graduates (interns) - could diagnose cases of periodontitis using a new system called the 2017 Periodontal Classification. A group of expert periodontists created a \"gold-standard\" diagnosis for comparison. We found that periodontists were the most accurate, while interns had more difficulty correctly identifying disease severity. Across all groups, many participants underestimated how serious the cases were. Participants were better at recognizing advanced disease compared to milder forms. When the participants repeated the diagnosis of the cases later, their accuracy improved, suggesting that practice and training help. Our results show that diagnosing gum disease can vary depending on exp","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"19 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of non-surgical periodontal treatment on Dickkopf-1 and secreted Frizzled-Related Protein 5 levels.","authors":"Sukran Acipinar,Kubilay Baris","doi":"10.1002/jper.70005","DOIUrl":"https://doi.org/10.1002/jper.70005","url":null,"abstract":"BACKGROUNDRecent advancements in bone tissue biomarker research have identified 2 promising molecules: Dickkopf-1 and secreted Frizzled-Related Protein 5. This study aims to evaluate the levels of these biomarkers in gingival crevicular fluid in periodontal health, gingivitis, and periodontitis and to assess the effects of non-surgical periodontal treatment on these biomarkers.METHODSA total of 99 adult subjects were included in this study, divided into 3 groups: 33 periodontally healthy individuals, 33 with gingivitis, and 33 with periodontitis. Patients in the gingivitis and periodontitis groups received non-surgical periodontal treatment. Periodontal clinical parameters were recorded, and gingival crevicular fluid levels of biomarkers were analyzed by enzyme-linked immunosorbent assay at baseline and 6-8 weeks post-treatment.RESULTSPre-treatment Dickkopf-1 levels were found to be highest in the periodontitis group (p &lt; 0.001). Conversely, secreted Frizzled-Related Protein 5 levels were highest in the healthy group (p &lt; 0.001). Post-treatment, a statistically significant reduction in Dickkopf-1 levels was observed in the gingivitis (p = 0.015) and periodontitis (p &lt; 0.001) groups, while secreted Frizzled-Related Protein 5 levels significantly increased (respectively, p = 0.008 and p &lt; 0.001). A statistically significant weak negative correlation was identified between total Dickkopf-1 and secreted Frizzled-Related Protein 5 levels (τ = -0.117, p = 0.027). Receiver operating characteristic curve analysis to assess diagnostic performance between periodontal health and periodontitis revealed an area under the curve of 0.938 for Dickkopf-1 and 0.803 for secreted Frizzled-Related Protein 5.CONCLUSIONSThese biomarkers could serve as valuable biomarkers in the pathogenesis of periodontal disease. Non-surgical periodontal treatment significantly affects the levels of these biomarkers, indicating their potential utility in monitoring therapeutic outcomes.PLAIN LANGUAGE SUMMARYIn the human body, bone tissue is in a state of constant balance of production and destruction. This balance supports the maintenance of the mechanical integrity of the skeleton and the regulation of calcium and phosphorus levels. Bone markers have been developed to monitor various bone diseases and the effect of treatments without any interventional procedures. Dickkopf-1 (Dkk-1) and secreted Frizzled-Related Protein 5 (sFRP5) are two of the current bone markers that play a role in the balance of bone formation and destruction in the human body. The presence of these molecules in periodontal diseases, which cause inflammation and bone destruction in the gingiva surrounding the teeth, is not yet clear. In this study, Dkk-1 and sFRP5 levels were investigated in periodontal diseases, and the effect of treatment of periodontal diseases on these molecules was evaluated. In the transition from periodontal disease to health, Dkk-1 levels decreased while sFRP5 levels increased. Consistent","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"35 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between Mediterranean diet and periodontal inflammation in a UK population: A cross-sectional study.","authors":"Giuseppe Mainas,Giuseppe Grosso,Jason Di Giorgio,Joshua Hurley,Meaad Mohammed Alamri,Gaetano Isola,Mark Ide,Luigi Nibali","doi":"10.1002/jper.70016","DOIUrl":"https://doi.org/10.1002/jper.70016","url":null,"abstract":"BACKGROUNDEvidence is emerging about the effects of a balanced nutrition in maintaining periodontal health. The aim of this project was to investigate the association between diet, severity of periodontitis, and periodontal and systemic inflammation.METHODSTwo hundred consecutive hospital patients underwent a full periodontal assessment, had blood samples taken, and filled out a food frequency questionnaire (FFQ). Adherence to a Mediterranean-type diet was assessed through the FFQ. High-sensitivity C-reactive protein (hs-CRP) serum levels of matrix metalloproteinases-8 (MMP-8), interleukin (IL)-1α, IL-1β, IL-6, IL-10, and IL-17 were analyzed. Correlation and multivariate logistic regression analyses were performed to test the relationships between dietary factors, inflammatory biomarkers, and clinical data.RESULTSA total of 195 patients had complete data, with 112 participants categorized as highly adherent to the Mediterranean diet. Multivariate analysis showed that low adherence to Mediterranean diet was associated to periodontitis stage III-IV (p = 0.055, odds ratio [OR] 0.35, 95% confidence interval [CI]: 0.12-0.89); among individual food groups, more frequent red meat consumption was independently associated with more severe periodontitis stage (p = 0.042, OR 2.75, 95% CI: 1.03-7.41). Periodontal disease severity showed moderate associations with both circulating hs-CRP and IL-6 in the univariate analysis, but only IL-6 association was confirmed after adjusting for confounders. Consumption of several plant-derived food groups was significantly inversely related to increased levels of hs-CRP, IL-1α, IL-6, IL-10, and IL-17.CONCLUSIONSThis study shows that low adherence to Mediterranean diet and higher red meat consumption may be associated with severity of periodontal disease. Studies with a larger sample size are needed to further clarify the current findings.PLAIN LANGUAGE SUMMARYThis study explored how everyday eating habits might impact gum health and overall inflammation. We evaluated 200 hospital patients by performing dental exams, taking blood samples, and asking them about their diets through questionnaires. In particular, we looked at how closely people followed a Mediterranean-style diet, known for its emphasis on fruits, vegetables, whole grains, and healthy fats. Our findings revealed that patients who did not follow the Mediterranean diet as closely tended to have more severe gum disease, especially if they consumed red meat frequently. We also observed that higher levels of a key inflammatory marker, such as interleukin-6 (IL-6), were linked to worse gum health, while diets rich in plant-based foods were associated with lower levels of various inflammatory markers. These results suggest that a balanced, Mediterranean-type diet could be important in reducing gum disease and systemic inflammation. Further studies with larger groups are needed to confirm these promising observations.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"34 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145059154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin inhibits orthodontically induced root resorption through YAP/P65/IL-6 signaling pathway.","authors":"Tian Wei,Jialin Liu,Peishen Chen,Jie Zhang,Dongyang Li,Liyan Liu,Runze Zhang,Chunmiao Jiang","doi":"10.1002/jper.24-0600","DOIUrl":"https://doi.org/10.1002/jper.24-0600","url":null,"abstract":"BACKGROUNDTo explore the effects and mechanisms of melatonin on orthodontically induced root resorption (OIIRR) by focusing its role on IL-6 secretion in human periodontal ligament cells (hPDLCs).METHODSCytokine array was used to identify the key inflammatory cytokine secreted by hPDLCs under excessive compressive force. Western blot analysis and immunohistochemistry staining (IHC) were conducted to examine the key proteins in the YAP/P65/IL-6 signaling pathway both in in vitro and in vivo experiments. Verteporfin, a YAP inhibitor, was used to confirm the involvement of p-YAP and its downstream signaling factor p-P65. Root resorption volume was assessed using micro-computed tomography (micro-CT).RESULTSCytokine array revealed that excessive compressive force significantly elevated interleukin-6 (IL-6) levels in hPDLCs. IHC indicated that both IL-6 and melatonin receptor 1 (MT1) were highly expressed in hPDLCs on the compressive side in the mouse OIIRR model. in vitro experiments demonstrated that the levels of p-YAP and p-P65 significantly increased when compressive force was applied to hPDLCs, and melatonin reversed this effect. Furthermore, Verteporfin produced effects similar to melatonin on IL-6 expression and the YAP/P65 signaling pathway. Micro-CT analysis showed noticeable root resorption in the mouse OIIRR model, which was significantly reduced following intraperitoneal injection of melatonin. IHC staining further confirmed that the YAP/P65/IL-6 signaling pathway was inhibited on the compressive side of the mouse OIIRR model after melatonin injection.CONCLUSIONSMelatonin was able to inhibit root resorption in the mouse OIIRR model and reduced IL-6 secretion in hPDLCs under compressive force by suppressing the YAP/P65 signaling pathway.PLAIN LANGUAGE SUMMARYThis study explored how melatonin, a natural hormone, protects against root resorption during orthodontic treatment. Specifically, we focused on how excessive compressive force induces IL-6 production and root resorption in both in vitro and in vivo experiments. in vitro, we found that applying compressive force to hPDLCs increased the release of IL-6, a molecule that promotes inflammation and bone resorption. Melatonin, however, reduced IL-6 levels by blocking the YAP/P65 signaling pathway. In vivo, we developed a mouse model of orthodontically induced root resorption, in which melatonin was injected intraperitoneally. Results showed that melatonin reduced root resorption and decreased IL-6 secretion in the periodontal tissues. Additionally, proteins of the YAP/P65 signaling pathway and the RANKL/OPG system were involved in vivo. Our findings suggest that melatonin could be a promising preventive approach to protect against root resorption during orthodontic treatment.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"310 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}