Journal of periodontology最新文献

{"title":"Triglyceride glucose index and periodontitis: A nationwide study","authors":"Yu‐Na Im, Na‐Yeong Kim, Ki‐Ho Chung","doi":"10.1002/jper.24-0490","DOIUrl":"https://doi.org/10.1002/jper.24-0490","url":null,"abstract":"BackgroundNon‐insulin‐based insulin resistance indices provide a cost‐effective assessment of insulin resistance. As periodontitis has a bidirectional relationship with diabetes, this study used these indices to analyze the association between insulin resistance and periodontitis and compared their diagnostic accuracy.MethodsThe study used healthcare big data from the Korea National Health and Nutrition Examination Survey (KNHANES, 2013–2018). Periodontitis was determined based on the Community Periodontal Index (CPI). Among the indices, triglyceride to high‐density lipoprotein cholesterol ratio (TG/HDL‐C), metabolic score for insulin resistance (METS‐IR), triglyceride glucose index (TyG), TyG adjusted with body mass index, TyG adjusted with waist circumference (WC), and TyG adjusted with waist‐to‐height ratio (WHtR) were compared. A multivariate logistic regression model was constructed by dividing the data into quartiles, and receiver operating characteristic (ROC) analysis was performed to compare the diagnostic accuracy.ResultsThe prevalence of periodontitis increased with increasing quartile of the insulin resistance index. The multivariate logistic regression analysis showed that insulin resistance and periodontitis were significantly associated in the fourth quartile for all indicators (<jats:italic>p</jats:italic> &lt; 0.05). In particular, the area under the curve (AUC) value of the TyG adjusted with WHtR was the highest at 0.64, implying that it had the best diagnostic accuracy among the compared indices.ConclusionTyG‐related indices were associated with the prevalence of periodontitis, and the TyG adjusted for WHtR showed the greatest diagnostic accuracy for periodontitis.Plain Language SummaryThis study looked at the association between a measure called the triglyceride glucose index (TyG) and periodontitis using data from 22,994 people in South Korea. The TyG helps to identify insulin resistance, a condition where the body does not respond well to insulin, which is crucial for controlling blood sugar levels. Unlike traditional tests that require blood samples and are time‐consuming, the TyG is simpler and cheaper. Our research found an association between insulin resistance and periodontitis. Among the different versions of the TyG, the one that combined the TyG with the waist‐to‐height ratio (TyG*WHtR) was the most accurate in identifying those who might have periodontitis. This means that using the TyG*WHtR could be a helpful way to find and help people who are at risk for insulin resistance and potentially improve their oral health early on.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"12 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The microbial metabolite isovaleric acid aggravates gelatinase‐mediated periodontal tissue destruction via the NF‐κB signaling pathway","authors":"Huiling Zheng, Ying Tu, Xinjie Ning, Qiang Guo, Biao Ren, Jing Xie, Chengcheng Liu","doi":"10.1002/jper.11375","DOIUrl":"https://doi.org/10.1002/jper.11375","url":null,"abstract":"BackgroundIsovaleric acid, an important short‐chain fatty acid, can be generated by subgingival microbial fermentation of a leucine‐rich diet, such as <jats:italic>Porphyromonas gingivalis</jats:italic>. This study aimed to explore the impact of isovaleric acid on the destruction of periodontal tissues and to investigate the underlying mechanisms.MethodsThe level of isovaleric acid in the saliva of clinical subjects was measured via mass spectrometry. Isovaleric acid local injection models in 5‐week‐old male Sprague Dawley rats and an isovaleric acid treatment model of human periodontal ligament stem cells (hPDLSCs) were established to explore the effect of isovaleric acid on periodontal tissue destruction and uncover the underlying mechanisms.ResultsOur study revealed that individuals with gingivitis and periodontitis presented significantly higher salivary levels of isovaleric acid than healthy controls. Local injections of isovaleric acid aggravated periodontal tissue damage and alveolar bone loss in rats. Moreover, isovaleric acid amplified the levels and activities of gelatinases, a process facilitated through the activation of NF‐κB (nuclear factor kappa‐B) signaling. Suppression of the NF‐κB pathway significantly reduced the isovaleric acid‐induced increase in gelatinases.ConclusionsOur findings demonstrate that isovaleric acid aggravates the destruction of periodontal tissue by modulating the levels and activities of gelatinases via the NF‐κB signaling pathway both in vitro and in vivo.Plain Language SummaryPeriodontitis is a chronic inflammatory disease characterized by the destruction of periodontal tissues, with oral bacteria as the progressive frontier. Isovaleric acid, an important short‐chain fatty acid, is a substance produced by oral bacteria during the breakdown of protein‐rich foods. By comparing saliva samples from healthy individuals and patients with periodontal diseases, we found that isovaleric acid levels were 2‒3 times higher in patients with inflamed or damaged periodontal tissue. In this study, we assessed the effects of isovaleric acid on periodontal tissues by injecting isovaleric acid into the gums of rats and treating human periodontal cells with isovaleric acid. We found that isovaleric acid worsened periodontal tissue damage and accelerated bone loss. This process worked through NF‐κB signaling pathway activation, which increased the activity and expression of gelatinases, enzymes that break down periodontal tissues. These findings increase our understanding of the possible involvement of isovaleric acid in the development of periodontitis. Targeting isovaleric acid metabolism may provide new approaches for the treatment or prevention of periodontitis.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"697 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of prenatal supplementation with probiotics on the development of experimental periodontitis in pregnant mice","authors":"Amanda F. Gonzalez, Pedro H. F. Silva, Cristhiam H. Martinez, Felipe V. S. Fabris, Sergio L. Salvador, Renato Casarin, Daniela B. Palioto, Flavia Furlaneto, Michel R. Messora","doi":"10.1002/jper.24-0597","DOIUrl":"https://doi.org/10.1002/jper.24-0597","url":null,"abstract":"BackgroundThis study assessed how prenatal probiotic (P) supplementation affected the development of experimental periodontitis (PE) in pregnant mice, as well as the anthropometric traits of the resulting fetuses and the placental and intestinal tissues of the mother.MethodsThirty‐two pregnant mice in all were divided into four groups (<jats:italic>n</jats:italic> = 8): Experimental periodontitis (PE), Experimental Periodontitis + Probiotics (PEP), Control + Probiotics (CP), and control (C). The animals in the PE and PEP groups received gavage with <jats:italic>Porphyromonas gingivalis</jats:italic> w83 for 15 days following pregnancy confirmation. Beginning 14 days prior to the animals' mating date, Groups CP and PEP were given <jats:italic>Bifidobacterium animalis</jats:italic> subsp. <jats:italic>lactis</jats:italic> HN019 until the day of euthanasia. On the 30th day of the experiment, all of the animals were sacrificed. Intestinal, periodontal, and placental tissues were gathered for immunoassay, microtomographic, and histomorphometric examinations.ResultsGroups C, CP, and PEP presented fetuses with higher weights compared to the PE group (<jats:italic>p </jats:italic>&lt; 0.05). Furthermore, The PE group showed smaller fetal size, a larger junctional zone in the placenta, a lower depth of intestinal crypts, and a lower bone volume when compared to all other experimental groups (<jats:italic>p</jats:italic> &lt; 0.05). In the immunoassay analysis, the PE group showed higher levels of pro‐inflammatory cytokine compared to C and CP groups. Additionally, the PEP group exhibited lower levels of pro‐inflammatory cytokines in gingival tissues (IL‐1β and TNF‐α) and placental tissues (IL‐1β) when compared to the PE group (<jats:italic>p</jats:italic> &lt; 0.05).ConclusionProbiotic supplementation in pregnant mice may attenuate the damage caused by PE to periodontal tissues, the placenta, intestines, and anthropometric characteristics of fetuses, as well as modulate the gingival and placental immunoinflammatory profile.Plain Language SummaryThis study investigated whether prenatal probiotics could protect pregnant mice and their fetuses from the harmful effects of periodontitis. Pregnant mice were divided into four groups based on whether they had gum disease, received probiotics, had both, or were in the control group. Periodontitis alone caused smaller, lighter fetuses, altered placentas, reduced jawbone volume, and thinner intestinal tissue. However, probiotic treatment improved these outcomes: fetuses were healthier, placentas and intestines showed less damage, and jawbones retained more bone. Probiotics also reduced inflammation in the gums and placenta. These results suggest that probiotics may help mitigate the negative effects of periodontitis during pregnancy, benefiting both the mother and the baby.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"21 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamins K2 and D3 enhance guided bone regeneration in rat calvarial bone defects","authors":"Gülce Nil Varlıhan, Ömer Birkan Ağralı, Hatice Selin Güngörmek, Sibel Demirci Delipınar, Leyla Kuru, Hafize Öztürk Özener","doi":"10.1002/jper.11372","DOIUrl":"https://doi.org/10.1002/jper.11372","url":null,"abstract":"BackgroundVitamins K2 and D3 exhibit anabolic effects on bone metabolism, but their effectiveness in guided bone regeneration (GBR) is unclear. The present study aimed to investigate the impacts of vitamins K2 and D3 administration on GBR of calvarial critical‐size defect (CCSD) in rats.MethodsA total of 48 defects were obtained by creating two bilateral 5 mm CCSDs in each of 24 rats: the right‐side defect was treated using a collagen‐based resorbable membrane and a bovine bone graft (GM defect), the left side remained empty (empty defect). The rats were assigned to four groups and administered distilled water (Control), vitamin K2 (K), vitamins K2 and D3 (KD), or vitamin D3 (D) for 8 weeks. Rats were sacrificed after 8 weeks. Histomorphometric and immunohistochemical analyses were performed.ResultsThe new bone area was greater in GM defects versus empty defects of all groups (&lt;jats:italic&gt;p&lt;/jats:italic&gt; &lt; 0.05), higher in vitamin groups than the control group in both defects (&lt;jats:italic&gt;p&lt;/jats:italic&gt; &lt; 0.05). Osteocalcin levels were elevated in GM defects than empty defects (&lt;jats:italic&gt;p&lt;/jats:italic&gt; &lt; 0.0001). Within GM defects, levels were higher in the vitamin groups compared with the control group (&lt;jats:italic&gt;p&lt;/jats:italic&gt; &lt; 0.05). Runx2 expression was increased in GM defects compared with empty defects and in vitamin groups compared with the control group (&lt;jats:italic&gt;p&lt;/jats:italic&gt; &lt; 0.05). IL‐1β levels were greater in empty defects compared with GM defects in vitamin groups (&lt;jats:italic&gt;p&lt;/jats:italic&gt; &lt; 0.05).ConclusionThe administration of vitamins K2 and D3 in GBR enhanced bone healing in CCSD of rats, indicating that the adjunctive use of these vitamins may represent a promising therapeutic strategy for bone regeneration.Plain Language SummaryVitamin K2 and vitamin D3 are known to play important roles in bone metabolism. Previous research revealed that vitamins K2 and D3 supplements might be effective as a therapeutic approach to bone regeneration. Although there are several methods of bone regeneration, each has limits. Thus, we aimed to investigate the effects of administering vitamins K2 and D3 alone or in combination to restore bone defects. In this study, each rat had two critical‐size cranial bone defects (defined as the smallest bone defect that cannot restore naturally throughout life); one was filled with bone grafts and barrier membranes, while the other was left empty. Then rats were divided into groups administered either distilled water, vitamin K2, vitamin D3 or both. More bone was found in the regenerated bone defects compared with those left empty and vitamin‐administered rats had more bone in defects than vitamin‐unadministered rats. Moreover, the highest amount of bone was observed in regenerated bone defects of rats that received combined vitamins K2 and D3. Therefore, we conclude that administration of vitamins K2 and D3 improves bone healing in critical‐size cranial bone defects in","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"8 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stromal cell‐derived factor‐1 regulates expression of vascular endothelial growth factor and osteopontin after tooth extraction","authors":"Jingjing Kong, Lei Li, Fei Gao, Kaiqi Zhang, Jianli Xie, Jun Cui","doi":"10.1002/jper.24-0785","DOIUrl":"https://doi.org/10.1002/jper.24-0785","url":null,"abstract":"BackgroundThe present study aimed to investigate the expression of stromal cell‐derived factor‐1 (SDF‐1) after tooth extraction in rats and its regulatory effect on the expression of osteopontin (OPN) and vascular endothelial growth factor (VEGF).MethodsThe first mandibular molar of the rats on the left was extracted, the histopathological changes of the extraction sockets were observed, and the SDF‐1 protein and mRNA expression were measured at 0, 1, 4, 7, 10, and 14 days. Furthermore, we used AMD3100 to block SDF‐1/CXC chemokine receptor 4 (CXCR4) signaling, and the expression changes of SDF‐1, OPN, and VEGF were observed.ResultsAt 1 day, the socket was filled with a blood clot. At 4 days, the granulation tissues were formed at the socket. At 7 days, the extraction socket was filled with mature fibrous connective tissue. At 10 days, a large amount of new trabecular bone was regenerated. At 14 days, the newly formed socket bone underwent remodeling. The expression level of SDF‐1 began to increase on the first day after tooth extraction and then peaked at 4 days. Then, SDF‐1 expression gradually declined and finally returned to the baseline level at 14 days. Moreover, the application of AMD3100 decreased the secretion of OPN and VEGF, both at protein and mRNA levels.ConclusionsThe expression of SDF‐1 increased first and then decreased after tooth extraction. SDF‐1 may play a regulatory role in the expression of OPN and VEGF.Plain Language SummaryTooth extraction is one of the most common operations that cause oral tissue defects and tooth loss. The healing of the tooth extraction wound affects the results of the later denture restoration and implant surgery. In this study, the expression of stromal cell‐derived factor‐1 (SDF‐1) increased first and then decreased after tooth extraction. It was found that after blocking the SDF‐1 signaling pathway, the expression levels of osteopontin (OPN) and vascular endothelial growth factor (VEGF) decreased correspondingly. While OPN was a marker of bone maturation, VEGF could promote angiogenesis and thus tissue repair. In conclusion, SDF‐1 may be involved in the process of tissue damage repair by regulating the expression of OPN and VEGF.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"49 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Papilla and gingival outcomes in implant‐rehabilitated patients with oligodontia: A retrospective study","authors":"Nicolas Dupré, Caroline Garou, Paul Monneyron, Rufino Felizardo, Maria Clotilde Carra, Benjamin PJ Fournier, Stephane Kerner","doi":"10.1002/jper.11369","DOIUrl":"https://doi.org/10.1002/jper.11369","url":null,"abstract":"BackgroundOligodontia is a rare condition characterized by the absence of at least 6 teeth. Implant‐supported prosthetic rehabilitation offers a reliable solution for affected patients. However, the unique periodontal traits of oligodontia raise questions about the best prosthetic approach for papilla reconstruction. This retrospective study aims to assess papilla and gingival esthetics in implant‐supported rehabilitation of patients with oligodontia. The study compared 3 prosthetic rehabilitation options: adjacent implants, bridges, and cantilevers.MethodsTwenty‐seven patients with oligodontia who received implant‐supported treatment participated in the study. Esthetic evaluations were performed using the pink esthetic score (PES) for teeth, implants, and pontics, while the papilla index score (PIS) was used to assess papilla appearance. Interdental sites were divided into 3 groups: control (papilla between teeth), tooth–papilla (papilla between tooth and pontic or implant), and implant–papilla (papilla between implants or between implant and pontic).ResultsThe study included 292 teeth, 116 implants, 27 pontics. The mean follow‐up for implants was 36.0 ± 19.8 months and for prosthetic rehabilitation, 26.7 ± 20.8 months. PES was highest for teeth (13.5 ± 1.04), followed by implants (12 ± 1.97) and pontics (11.9 ± 2.04) (&lt;jats:italic&gt;p&lt;/jats:italic&gt; &lt; 0.001). PIS demonstrated a difference (&lt;jats:italic&gt;p&lt;/jats:italic&gt; &lt; 0.001) between control (2.72 ± 0.64), tooth–papilla (2.17 ± 0.86), and implant–papilla (1.26 ± 0.84). No statistical difference was observed between the 2 implants and between an implant and pontic.ConclusionNatural teeth consistently demonstrated superior esthetic outcomes in PES. However, papilla regeneration was more successful when a tooth was involved in the prosthetic restoration. Reversely, when required, 2 single‐crown implant‐supported and cantilever implant‐supported are similarly effective in papilla outcomes.Plain language summaryOligodontia, a rare condition characterized by the absence of at least 6 teeth, presents challenges in achieving natural‐looking dental restorations. This study evaluated the esthetic outcomes of implant‐supported prosthetic treatments in 27 patients with oligodontia, focusing on interdental papilla reconstruction. Three rehabilitation methods were compared: adjacent implants, bridges, and cantilevers. Esthetic assessments used the pink esthetic score (PES) for teeth, implants, and pontics, and the papilla index to measure papilla quality. Results showed that natural teeth achieved the highest PESs, followed by implants and pontics. For papilla quality, sites involving natural teeth scored better than those between 2 implants or between an implant and a pontic. Our findings emphasize that natural teeth presence in prosthetic designs improves esthetic outcomes, particularly in papilla regeneration. However, when incorporating natural teeth is not an option, adjacent crown‐supported implant","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"10 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and histological outcomes of allogenic amnion–chorion membrane for palatal donor site healing","authors":"Chun‐Teh Lee, Edidiong Nosakhare Umoh, Asfia Husain, Nooshin Gomnam, Gena Tribble, Bing‐Yan Wang, Seiko Min","doi":"10.1002/jper.11370","DOIUrl":"https://doi.org/10.1002/jper.11370","url":null,"abstract":"BackgroundThis pilot split‐mouth randomized controlled trial aimed to compare the effectiveness of an allogenic amnion–chorion membrane (ACM) in promoting clinical and histological palatal donor site wound healing to a collagen dressing (CLD).MethodsTwo palatal donor sites (right and left) of 19 participants requiring free gingival graft (FGG) procedures were randomly assigned to receive either ACM (test group) or CLD (control group). Clinical outcomes, including the size of the wound area, the degree of epithelialization, color match, bleeding condition, pain, and sensibility, were measured. Histomorphometric analyses were also performed to compare the effectiveness of the dressing in each group. Outcomes were evaluated 4, 10, 14, 21, 28, 45, and 60 days post‐operatively.ResultsThere were no significant differences in the reduction of wound area size, the degree of epithelialization, color change, pain, sensibility, thickness of the epithelium and keratin layer, number of inflammatory cells, fibroblasts and blood vessels, and density of collagen between ACM and CLD palatal donor sites during all the evaluations.ConclusionsThe effectiveness of ACM in supporting wound healing at the palatal FGG donor site was comparable to that of CLD.Clinical Trial RegistrationThis study was registered with ClinicalTrials.gov (ID NCT03713073). The title of the trial is Clinical and Histological Outcomes of Allogenic Amnion Chorion Membrane in the Healing of Free Gingival Graft Donor Site.Plain language summaryThis study looked at how well 2 different treatments help wounds heal after a free gingival graft procedure, which requires taking donor tissue from the palate. Nineteen participants had 2 areas on their palates treated: 1 with an allogenic amnion–chorion membrane (ACM) and the other with a collagen dressing. The study compared the healing process by looking at the size of the wound, the kinetics of the wound healed, the pain experienced, and the tissue's appearance. It also examined the treated sites under a microscope to assess the number of cells involved in the healing process. After evaluating the healing progress at several time points (from 4 to 60 days after surgery), no significant differences between the 2 treatments were found in terms of clinical or microscopic healing outcomes. In conclusion, ACM was found to be as effective as the collagen dressing in promoting healing at the donor site, meaning both treatments can be considered equally viable options for this procedure.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"3 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resolvin E1 and maresin 1 restore senescence‐induced disruption of human periodontal ligament fibroblast function","authors":"Ozge Unlu, Zeliha Guney, Alpdogan Kantarci","doi":"10.1002/jper.24-0565","DOIUrl":"https://doi.org/10.1002/jper.24-0565","url":null,"abstract":"BackgroundAging impairs the mechanisms that regulate inflammation, resulting in low‐level chronic inflammation even in the absence of infection and increasing the risk of developing age‐related illnesses. Periodontal ligament fibroblasts (PDLF) are responsible for wound healing and periodontal tissue regeneration. Periodontal inflammation disrupts PDLF function, which may be exacerbated by aging. We tested the hypothesis that senescence‐induced changes in PDLF will be reversed by specialized mediators of resolution of inflammation‐resolvin E1 (RvE1) and maresin 1 (MaR1).MethodsPrimary human PDLFs were cultured with D‐galactose to induce senescence. The senescence was confirmed with a senescence‐associated beta‐galactosidase assay. The impact of senescence on cell viability, proliferation, wound healing, cell cycle, type I collagen expression, oxidative stress, inflammatory profiles, and growth factor production was evaluated. We measured the specialized pro‐resolving mediators (SPM)‐mediated effects on senescent PDL fibroblasts by treating them with 100 nM RvE1 or 100 nM MaR1 or the vehicle.ResultsD‐galactose treatment significantly increased senescence, oxidative stress, and inflammation while it delayed wound closure and reduced cell viability and proliferation on PDLFs (<jats:italic>p</jats:italic> &lt; 0.05). RvE1 or MaR1 treatment significantly decreased β‐galactosidase expression and inflammation, restored cell viability, increased cell proliferation, and accelerated wound closure (<jats:italic>p</jats:italic> &lt; 0.05). MaR1 demonstrated a more potent impact on reversing the senescence and regenerative effect than RvE1.ConclusionRvE1 and MaR1 reversed the senescence‐induced changes in primary PDLFs, restoring wound healing capacity and function.Plain Language SummaryAging may induce periodontal inflammation, which interferes with the activity of the periodontal ligament fibroblasts (PDLFs). We measured the effect of specific mediators of resolution of inflammation, resolvin E1 (RvE1) and maresin 1 (MaR1), on aging in PDLFs. Treatment with RvE1 or MaR1 significantly reduced inflammation and senescence and restored cell proliferation, wound closure, and cell viability.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"41 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiscale, interspecies evaluation of parameters affecting alveolar bone repair.","authors":"Pavla Ticha,Arush Joshi,Julia S Dworan,Marzia Armaro,Masaki Arioka,Quanchen Xu,Igor Pilawski,Yindong Liu,Chih-Hao Chen,Xibo Pei,Ustun Serdar Tulu,Hannes Traxler,Yasemin Erdogan,Jill A Helms","doi":"10.1002/jper.24-0594","DOIUrl":"https://doi.org/10.1002/jper.24-0594","url":null,"abstract":"BACKGROUNDWe undertook a comparative analysis of bone healing in the craniomaxillofacial (CMF) skeleton, focusing on the extent to which the physiological processes of bone repair and remodeling differed between different species. Understanding these differences can enhance the value of research outputs and thus improve treatment strategies.METHODSAn osteotomy was produced in an equivalent anatomical location in mice, rats, minipigs, and human patients. Quantitative analyses were performed using micro-computed tomographic (µCT) imaging, histology, dynamic histomorphometry, immunohistochemistry, and cellular activity assays.RESULTSNon-invasive imaging analyses, histomorphometry, and molecular and cellular analyses together demonstrated that the overall bone repair program was highly conserved between rodents and minipigs, correlating with limited human data. Comparing the rate of bone repair across species was confounded by limited time points for analysis in human tissues. However, at later time points, CMF bone repair in minipigs was equivalent to CMF bone repair in rodents.CONCLUSIONSThe molecular and cellular features of bone repair are shared among large and small animals, suggesting a common bone healing mechanism among species. Combined with the availability of transgenic lines and an extensive array of molecular and cellular analyses, this makes rodents an appealing choice for preclinical testing.PLAIN LANGUAGE SUMMARYWe conducted this study to understand how bone heals in the head skeleton. We compared this repair process in animal models, including mice, rats, and minipigs, against a limited set of data from humans. Our goal was to determine whether bone repair and remodeling were conserved between species because this would have implications for preclinical studies to improve treatment strategies. We created a controlled injury in similar bone areas in each species, then examined the healing process using various research techniques, analyzing bone architecture and cellular activity. Our findings showed that the overall process of bone healing was similar between species. Although we had limited data from humans at later stages of healing, the healing patterns in minipigs closely matched those in rodents at the later stages. These similarities suggest that the way bones heal is equivalent among mammals. Therefore, with the added advantage of advanced research techniques available, rodents are an excellent choice for testing new treatments before advancing to studies in large animals and testing in humans.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"47 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144568614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of horizontal platelet‐rich fibrin on gingival tissue regeneration: Cellular and histological analysis","authors":"Jihua Chai, Yulu Chen, Shan Shi, Zaibo Yang, Sensen Li, Richard J. Miron, Yulan Wang, Yufeng Zhang","doi":"10.1002/jper.11364","DOIUrl":"https://doi.org/10.1002/jper.11364","url":null,"abstract":"BackgroundHorizontal platelet‐rich fibrin (H‐PRF) is a novel platelet concentrate known for promoting tissue regeneration, the purpose of this study was to assess the impact of H‐PRF on gingival defects healing using both in vitro and in vivo approaches.MethodsHuman gingival fibroblasts (hGFs) were cultured with lipopolysaccharide (LPS), H‐PRF, and H‐PRF + LPS. Then, cell viability, proliferation, apoptosis and migration were evaluated via fluorescence staining, CCK‐8, flow cytometry, scratch and Transwell assays, respectively. Gingival defects were created in a rabbit model and treated with or without H‐PRF membranes. Tissue remodeling was evaluated at 14 and 21 days post‐surgery using hematoxylin and eosin (H&amp;E) staining, Masson's trichrome staining, and immunohistochemistry (IHC) for E‐cadherin, and neovascularization was evaluated by IHC for CD31.ResultsH‐PRF promoted hGFs proliferation and migration and mitigated the negative effects of LPS on hGF viability and apoptosis. In animal model, H‐PRF significantly accelerated gingival defect closure, reduced pseudomembrane formation, and promoted the formation of a thicker, stratified squamous epithelium with rete peg formation by day 21. Compared with untreated defects, H‐PRF‐treated defects exhibited significantly increased collagen deposition and elevated expression of E‐cadherin. Moreover, enhanced neovascularization was observed in the H‐PRF group, as evidenced by the increased expression of CD31‐positive cells.ConclusionH‐PRF significantly improved gingival defects healing by enhancing hGF cellular behaviors and promoting epithelialization and neovascularization. These findings support the potential of H‐PRF as an effective adjunctive therapy during gingival tissue regeneration.Plain Language SummaryHorizontal platelet‐rich fibrin (H‐PRF) is a platelet concentrate derived from patient's own blood that has gained momentum in regenerative medicine due to its ability to support tissue repair. In our study, we compared the healing process of gingival defects treated with H‐PRF to those that healed naturally and found that the defects treated with H‐PRF healed more quickly and more completely. The treated areas contained stronger and more organized tissue, with improved blood flow. H‐PRF also helps the cells in the gums move to the injury site more effectively, which is important for faster and more complete healing. Our findings suggest that H‐PRF could be a useful tool to enhance the outcomes of soft tissue augmentation around natural teeth or implants, which would lead to healthier and stronger gingiva for patients.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"247 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}