Journal of periodontal research最新文献

{"title":"Effect of low-intensity pulsed ultrasound on the mineralization of force-treated cementoblasts and orthodontically induced inflammatory root resorption via the Lamin A/C-Yes associated protein axis","authors":"Fu Zheng,&nbsp;Tong Wu,&nbsp;Feifei Wang,&nbsp;Hongyi Tang,&nbsp;Xinyu Cui,&nbsp;Duo Liu,&nbsp;Peng Chen,&nbsp;Jiangfeng Fu,&nbsp;Cuiying Li,&nbsp;Jiuhui Jiang","doi":"10.1111/jre.13330","DOIUrl":"10.1111/jre.13330","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Orthodontic treatment commonly results in orthodontically induced inflammatory root resorption (OIIRR). This condition arises from excessive orthodontic force, which triggerslocal inflammatory responses and impedes cementoblasts' mineralization capacity. Low-intensity pulsed ultrasound (LIPUS) shows potential in reducing OIIRR. However, the precise mechanisms through which LIPUS reduces OIIRR remain unclear. This study aimed to explore the effects and mechanisms of LIPUS on the mineralization of force-treated cementoblasts and its impact on OIIRR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We established a rat OIIRR model and locally administered LIPUS stimulation for 7 and 14 days. We analyzed root resorption volume, osteoclast differentiation, and the expression of osteocalcin and yes-associated protein 1 (YAP1) using micro-computed tomography (micro-CT), hematoxylin and eosin, tartrate-resistant acid phosphatase, immunofluorescence and immunohistochemistry staining. In vitro, we applied compressive force and LIPUS to the immortalized mouse cementoblasts (OCCM30). We assessed mineralization using alkaline phosphatase (ALP) staining, alizarin red staining, real-time quantitative polymerase chain reaction, Western blotting and immunofluorescence staining.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In rats, LIPUS reduced OIIRR, as evidenced by micro-CT analysis and histological staining. In vitro, LIPUS enhanced mineralization of force-treated OCCM30 cells, as indicated by ALP and alizarin red staining, upregulated mRNA expression of mineralization-related genes, and increased protein expression of mineralization markers. Mechanistically, LIPUS activated YAP1 signaling via the cytoskeleton-Lamin A/C pathway, supported by immunofluorescence and Western blot analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study demonstrates that LIPUS promotes mineralization in force-treated cementoblasts and reduces OIIRR by activating YAP1 through the cytoskeletal-Lamin A/C signaling pathway. These findings provide fresh insights into how LIPUS benefits orthodontic treatment and suggest potential strategies for preventing and treating OIIRR.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"60 2","pages":"189-199"},"PeriodicalIF":3.4,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141878924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of oral biomarkers during early healing at augmented dental implant sites.","authors":"Lorenzo Tavelli, Shayan Barootchi, Maria Vera Rodriguez, Jim Sugai, David T Wu, Ning Yu, William V Giannobile","doi":"10.1111/jre.13328","DOIUrl":"https://doi.org/10.1111/jre.13328","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study is to assess early wound healing expression of local angiogenic biomarkers following connective tissue graft (CTG) at dental implant sites.</p><p><strong>Methods: </strong>Twenty-eight subjects with single dental implants exhibiting a soft tissue dehiscence were included and randomly treated with CTG, either with coronally advanced flap (CAF) or with tunnel technique (TUN). Peri-implant crevicular fluid (PICF) was collected at the midfacial and midlingual aspect of the implant sites at baseline and at 3, 7, 14, 30, and 90 days after the surgical intervention. The expression of angiogenin (ANG), fibroblast growth factor-2 (FGF-2), platelet-derived growth factor (PDGF), tissue inhibitor of metalloproteinases-2 (TIMP-2), and vascular endothelial growth factor (VEGF) was investigated over a period of 3 months. Patient-reported outcomes, clinical measurements, and ultrasonography scans at multiple time points were also evaluated.</p><p><strong>Results: </strong>The longitudinal regression revealed a significant difference in the expression of VEGF and TIMP-2 between CAF- and TUN-treated sites over 3 months (p = .033 and p = .004, respectively), whereas no significant differences were observed for ANG, FGF-2 and PDGF between the two groups. At 7 days, a direct correlation was observed between ANG levels and ultrasonographic color velocity in the CAF group (p < .001) and between ANG levels and ultrasonographic color power in the TUN group (p = .028). VEGF levels and ultrasonographic mean perfused area of the CTG were significantly correlated at the 7-day time point (p < .001 for both CAF and TUN). The expression of VEGF at 7 days was directly associated with mucosal thickness gain at 1 year (p < .001 for both groups). Early TIMP-2 expression showed an inverse correlation with time to recovery (p = .002). TIMP-2 levels at 3 months exhibited inverse correlations with mean dehiscence coverage (p = .004) and the rate of complete dehiscence coverage (p = .012).</p><p><strong>Conclusion: </strong>PICF biomarkers can be used to monitor early wound healing events following soft tissue grafting at implant sites. VEGF and TIMP-2 showed correlations with the 1-year clinical and volumetric outcomes, as well as with post-operative patient-reported outcomes and Doppler Ultrasonographic tissue perfusion-related parameters.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patients' awareness of the association between periodontitis and other diseases: Still a long way to go","authors":"Maria Clotilde Carra,&nbsp;Viet-Thi Tran,&nbsp;Philippe Bouchard","doi":"10.1111/jre.13329","DOIUrl":"10.1111/jre.13329","url":null,"abstract":"&lt;p&gt;Periodontitis is a chronic inflammatory disease of the tooth supporting tissues associated with a dysbiotic oral microbiota. Evidence has shown that periodontitis is associated with an increased risk of development and progression of several other chronic diseases, such as type-2 diabetes, atherosclerotic and cardiometabolic diseases.&lt;span&gt;&lt;sup&gt;1-3&lt;/sup&gt;&lt;/span&gt; Common immune-inflammatory pathways and the role of periodontal pathogens are the most probable factors explaining the link between periodontitis and other diseases.&lt;span&gt;&lt;sup&gt;4, 5&lt;/sup&gt;&lt;/span&gt; While a substantial amount of literature informed the medical community about this link, little is known about the patient's awareness. This study aimed to investigate the level of awareness about the link between periodontitis and other diseases in a weighted data set representative of patients living with chronic conditions in France.&lt;/p&gt;&lt;p&gt;We analyzed data from a self-reported questionnaire on oral and periodontal health sent to all participants from the Community of Patients for Research (ComPaRe), a nationwide cohort of patients with at least one chronic disease (defined as a condition requiring health care for at least 6 months), in France. Participants are regularly followed up using patient-reported outcome and experience measurements. For this cross-sectional analysis, we used a data set obtained by calibration on margins with sex-specific weights for age and educational level derived from National Census data describing the French population reporting at least one chronic condition.&lt;span&gt;&lt;sup&gt;6&lt;/sup&gt;&lt;/span&gt; The ComPaRe study was approved by the local authority (IRB No. 0008367).&lt;/p&gt;&lt;p&gt;The online questionnaire included the evaluation of periodontitis risk (French-validated version of the CDC/AAP questionnaire),&lt;span&gt;&lt;sup&gt;7&lt;/sup&gt;&lt;/span&gt; and oral health-related quality of life (OHIP-14).&lt;span&gt;&lt;sup&gt;8&lt;/sup&gt;&lt;/span&gt; Severe periodontitis was assessed by calculating the Periodontal Screening Score (PESS) on selected questions, age and smoking.&lt;span&gt;&lt;sup&gt;7&lt;/sup&gt;&lt;/span&gt; Awareness was evaluated via a specific question asking the patients whether they have been informed, and by whom, about the link between periodontitis and general health. We described the answer to the questionnaire and studied the factors associated with the awareness of the link between periodontitis and other diseases. Statistical analyses were performed using SPSS (version 29·0; SPSS Inc., Chicago, Illinois, USA).&lt;/p&gt;&lt;p&gt;We included 8537 adults in the analysis (response rate: 33%). In the weighted data set, mean age was 56.34 years (SD: 16.99), 59.8% were affected by two or more chronic diseases, 13.5% were current smokers, and 45.6% were former smokers (Table S1). Overall, 56.4% reported to have visited their dentists and 43.3% to have received a scaling within the previous year. Only 2230 (26.1%) patients reported to be aware of a link between periodontitis and other diseases, with the highest level of awareness among patients","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"60 2","pages":"200-202"},"PeriodicalIF":3.4,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jre.13329","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging therapeutic strategies targeting bone signaling pathways in periodontitis","authors":"Araceli Valverde,&nbsp;Anne George,&nbsp;Salvador Nares,&nbsp;Afsar R. Naqvi","doi":"10.1111/jre.13326","DOIUrl":"10.1111/jre.13326","url":null,"abstract":"<p>Periodontitis is a multifactorial immune-mediated disease exacerbated by dysregulated alveolar bone homeostasis. Timely intervention is crucial for disease management to prevent tooth loss. To successfully manage periodontitis, it is imperative to understand the cellular and molecular mechanisms involved in its pathogenesis to develop novel treatment modalities. Non-surgical periodontal therapy (NSPT) such as subgingival instrumentation/debridement has been the underlying treatment strategy over the past decades. However, new NSPT approaches that target key signaling pathways regulating alveolar bone homeostasis have shown positive clinical outcomes. This narrative review aims to discuss endogenous bone homeostasis mechanisms impaired in periodontitis and highlight the clinical outcomes of preventive periodontal therapy to avoid invasive periodontal therapies. Although the anti-resorptive therapeutic adjuncts have demonstrated beneficial outcomes, adverse events have been reported. Diverse immunomodulatory therapies targeting the osteoblast/osteoclast (OB/OC) axis have shown promising outcomes in vivo. Future controlled randomized clinical trials (RCT) would help clinicians and patients in the selection of novel preventing therapies targeting key molecules to effectively treat or prevent periodontitis.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"60 2","pages":"101-120"},"PeriodicalIF":3.4,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jre.13326","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141752026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of three artificial intelligence (AI)-based large language models in standardized testing; implications for AI-assisted dental education","authors":"Hamoun Sabri,&nbsp;Muhammad H. A. Saleh,&nbsp;Parham Hazrati,&nbsp;Keith Merchant,&nbsp;Jonathan Misch,&nbsp;Purnima S. Kumar,&nbsp;Hom-Lay Wang,&nbsp;Shayan Barootchi","doi":"10.1111/jre.13323","DOIUrl":"10.1111/jre.13323","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Introduction&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;The emerging rise in novel computer technologies and automated data analytics has the potential to change the course of dental education. In line with our long-term goal of harnessing the power of AI to augment didactic teaching, the objective of this study was to quantify and compare the accuracy of responses provided by ChatGPT (GPT-4 and GPT-3.5) and Google Gemini, the three primary large language models (LLMs), to human graduate students (control group) to the annual in-service examination questions posed by the American Academy of Periodontology (AAP).&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Under a comparative cross-sectional study design, a corpus of 1312 questions from the annual in-service examination of AAP administered between 2020 and 2023 were presented to the LLMs. Their responses were analyzed using chi-square tests, and the performance was juxtaposed to the scores of periodontal residents from corresponding years, as the human control group. Additionally, two sub-analyses were performed: one on the performance of the LLMs on each section of the exam; and in answering the most difficult questions.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;ChatGPT-4 (total average: 79.57%) outperformed all human control groups as well as GPT-3.5 and Google Gemini in all exam years (&lt;i&gt;p&lt;/i&gt; &lt; .001). This chatbot showed an accuracy range between 78.80% and 80.98% across the various exam years. Gemini consistently recorded superior performance with scores of 70.65% (&lt;i&gt;p&lt;/i&gt; = .01), 73.29% (&lt;i&gt;p&lt;/i&gt; = .02), 75.73% (&lt;i&gt;p&lt;/i&gt; &lt; .01), and 72.18% (&lt;i&gt;p&lt;/i&gt; = .0008) for the exams from 2020 to 2023 compared to ChatGPT-3.5, which achieved 62.5%, 68.24%, 69.83%, and 59.27% respectively. Google Gemini (72.86%) surpassed the average scores achieved by first- (63.48% ± 31.67) and second-year residents (66.25% ± 31.61) when all exam years combined. However, it could not surpass that of third-year residents (69.06% ± 30.45).&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusions&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Within the confines of this analysis, ChatGPT-4 exhibited a robust capability in answering AAP in-service exam questions in terms of accuracy and reliability while Gemini and ChatGPT-3.5 showed a weaker performance. These findings underscore the potential of deploying LLMs as an educational tool in periodontics and oral implantology domains. However, the current limitations of these models such as inability to effectively process image-based inquiries, the propensity for generating inconsistent responses to the same prompts, and achieving ","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"60 2","pages":"121-133"},"PeriodicalIF":3.4,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jre.13323","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbial dysbiosis and inflammation: Impact on periodontal health","authors":"Yifan Zhao,&nbsp;Yi Liu,&nbsp;Lu Jia","doi":"10.1111/jre.13324","DOIUrl":"10.1111/jre.13324","url":null,"abstract":"<p>Periodontitis is widely acknowledged as the most prevalent type of oral inflammation, arising from the dynamic interplay between oral pathogens and the host's immune responses. It is also recognized as a contributing factor to various systemic diseases. Dysbiosis of the oral microbiota can significantly alter the composition and diversity of the gut microbiota. Researchers have delved into the links between periodontitis and systemic diseases through the “oral–gut” axis. However, whether the associations between periodontitis and the gut microbiota are simply correlative or driven by causative mechanistic interactions remains uncertain. This review investigates how dysbiosis of the gut microbiota impacts periodontitis, drawing on existing preclinical and clinical data. This study highlights potential mechanisms of this interaction, including alterations in subgingival microbiota, oral mucosal barrier function, neutrophil activity, and abnormal T-cell recycling, and offers new perspectives for managing periodontitis, especially in cases linked to systemic diseases.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"60 1","pages":"30-43"},"PeriodicalIF":3.4,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytokine responses of CD4+ T cells and NKT cells to periodontitis-associated bacteria in individuals with or without periodontitis","authors":"Anne Katrine Danielsen,&nbsp;Laura Massarenti,&nbsp;Lia Minculescu,&nbsp;Peter Østrup Jensen,&nbsp;Peter Riis Hansen,&nbsp;Palle Holmstrup,&nbsp;Christian Damgaard,&nbsp;Claus Henrik Nielsen","doi":"10.1111/jre.13317","DOIUrl":"10.1111/jre.13317","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Aim&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Periodontitis is an inflammatory disease driven by opportunistic bacteria including &lt;i&gt;Porphyromonas gingivalis&lt;/i&gt; and &lt;i&gt;Fusobacterium nucleatum&lt;/i&gt;, where T-cell and NKT-cell responses to these bacteria in patients with periodontitis grade B or C are not fully elucidated. The objective is to determine if exaggerated proinflammatory Th-cell responses to periodontitis-associated bacteria, but not commensal bacteria, is a characteristic of increased periodontitis grade.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Mononuclear cells from patients with periodontitis grade C (&lt;i&gt;n&lt;/i&gt; = 26) or grade B (&lt;i&gt;n&lt;/i&gt; = 33) and healthy controls (HCs; &lt;i&gt;n&lt;/i&gt; = 26) were stimulated with &lt;i&gt;P. gingivalis&lt;/i&gt;, &lt;i&gt;F. nucleatum&lt;/i&gt; or the commensal bacteria, &lt;i&gt;Staphylococcus epidermidis&lt;/i&gt; and &lt;i&gt;Cutibacterium acnes.&lt;/i&gt; Cytokine production by different T-cell populations and FOXP3-expression by regulatory T cells were assessed by flow cytometry.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Compared to HCs, grade C patients had decreased frequencies of interleukin (IL)-10-producing CD4+ T cells before stimulation (&lt;i&gt;p&lt;/i&gt; = .02) and increased frequencies of IFN-y-producing CD4+ T cells after stimulation with &lt;i&gt;P. gingivalis&lt;/i&gt; (&lt;i&gt;p&lt;/i&gt; = .0019). Grade B patients had decreased frequencies of FOXP3+ CD4+ T cells before (&lt;i&gt;p&lt;/i&gt; = .030) before and after stimulation with anti-CD2/anti-CD3/anti-CD28-loaded beads (&lt;i&gt;p&lt;/i&gt; = .047), &lt;i&gt;P. gingivalis&lt;/i&gt; (&lt;i&gt;p&lt;/i&gt; = .013) and &lt;i&gt;S. epidermidis&lt;/i&gt; (&lt;i&gt;p&lt;/i&gt; = .018). Clinical attachment loss correlated with the frequencies of IFN-y-producing Th1 cells in &lt;i&gt;P. gingivalis&lt;/i&gt;- and &lt;i&gt;F. nucleatum&lt;/i&gt;-stimulated cultures in grade B patients (&lt;i&gt;p&lt;/i&gt; = .023 and &lt;i&gt;p&lt;/i&gt; = .048, respectively) and with the frequencies of Th17 cells in &lt;i&gt;P. gingivalis&lt;/i&gt;-stimulated cultures (&lt;i&gt;p&lt;/i&gt; = .0062) in grade C patients. Patients with periodontitis grade C or grade B showed lower frequencies of IL-10-producing NKT cells than HCs in unstimulated cultures (&lt;i&gt;p&lt;/i&gt; = .0043 and &lt;i&gt;p&lt;/i&gt; = .027 respectively).&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusions&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Both periodontitis groups showed decreased frequencies of immunoregulatory T-cell and NKT cell subsets at baseline. Clinical attachment loss correlated with &lt;i&gt;P. gingivalis-&lt;/i&gt;induced Th17-responses in grade C patients and with Th1-responses in grade B patients when cells were stimulated with &lt;i&gt;P. gingivalis&lt;/i&gt;, supporting that dysregulated pro-inflammatory T-cell responses to periodontitis-associated bacteria contribute to the pathogene","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"60 2","pages":"177-188"},"PeriodicalIF":3.4,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jre.13317","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Native vs. ribosome-crosslinked collagen membranes for periodontal regeneration: A randomized clinical trial","authors":"Yiwei Wang,&nbsp;Yiru Xia,&nbsp;Jielei Qian,&nbsp;Yufeng Xie,&nbsp;Rong Shu,&nbsp;Zhikai Lin","doi":"10.1111/jre.13321","DOIUrl":"10.1111/jre.13321","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To evaluate whether the ribosome-crosslinked collagen membrane (RCCM) is non-inferior to the natural collagen membrane (NCM) used in regeneration surgery in terms of clinical attachment level (CAL) gain at 6 months.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Eighty patients diagnosed as generalized periodontitis presenting with isolated infrabony defect (≥4 mm deep) were enrolled and randomized to receive regenerative surgery, either with NCM or RCCM, both combined with deproteinized bovine bone mineral (DBBM). CAL, pocket probing depth (PPD), and gingival recession (GR) were recorded at baseline, 3, and 6 months postoperatively. Periapical radiographs were taken at baseline, immediately, and 6 months after surgery. Early wound healing index (EHI) and patients' responses were recorded at 2 weeks postoperatively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>At 6 months post-surgery, the mean CAL gain was 3.1 ± 1.5 mm in the NCM group and 2.9 ± 1.5 mm in the RCCM group, while the mean PPD was 4.3 ± 1.1 mm in the NCM group and 4.2 ± 1.0 mm in the RCCM group. Both groups demonstrated a statistically significant improvement from the baseline (<i>p</i> &lt; .01). RCCM was non-inferior to NCM concerning the primary outcome (CAL gain at 6 months). The GR at 6 months postoperatively was 1.3 ± 1.2 and 1.2 ± 1.1 mm, which showed no difference compared with baseline. At 6 months follow-up, the radiographic linear bone fill (RLBF) was 6.5 ± 2.8 and 5.5 ± 2.6 mm (<i>p</i> &gt; .05), while the bone fill percentage (BF%) was 102.3 ± 53.5% and 92.3 ± 40.1% (<i>p</i> &gt; .05), in the NCM and RCCM groups, respectively. There was no significant difference in EHI and postoperative responses between two groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>RCCM + DBBM resulted in no-inferior clinical and radiographic outcomes to NCM + DBBM for the treatment of isolated infrabony defect in 6 months.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"60 2","pages":"154-165"},"PeriodicalIF":3.4,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of oxidized LDL versus IL-1ß/TNF-ɑ/INFɣ on human gingival mesenchymal stem cells properties","authors":"Karim Fawzy El-Sayed,&nbsp;Elena Mahlandt,&nbsp;Kristina Schlicht,&nbsp;Kim Enthammer,&nbsp;Johannes Tölle,&nbsp;Juliane Wagner,&nbsp;Katharina Hartmann,&nbsp;Peter R. Ebeling,&nbsp;Christian Graetz,&nbsp;Mathias Laudes,&nbsp;Christof E. Dörfer,&nbsp;Dominik M. Schulte","doi":"10.1111/jre.13319","DOIUrl":"10.1111/jre.13319","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Oxidized low-density lipoprotein (oxLDL) is an important player in the course of metabolic inflammatory diseases. oxLDL was identified in the gingival crevicular fluid, denoting possible associations between oxLDL-induced inflammation and periodontal disease. The current investigation compared for the first-time direct effects of oxLDL to a cytokine cocktail of IL-1ß/TNF-ɑ/INF-γ on gingival mesenchymal stem cells' (G-MSCs) attributes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Human third passage G-MSCs, isolated from connective tissue biopsies (<i>n</i> = 5) and characterized, were stimulated in three groups over 7 days: control group, cytokine group (IL-1β[1 ng/mL], TNF-α[10 ng/mL], IFN-γ[100 ng/mL]), or oxLDL group (oxLDL [50 μg/mL]). Next Generation Sequencing and KEGG pathway enrichment analysis, stemness gene expression (<i>NANOG/SOX2/OCT4A</i>), cellular proliferation, colony-formation, multilinear potential, and altered intracellular pathways were investigated via histochemistry, next-generation sequencing, and RT-qPCR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>G-MSCs exhibited all mesenchymal stem cells' characteristics. oxLDL group and cytokine group displayed no disparities in their stemness markers (<i>p</i> &gt; .05). Next-generation-sequencing revealed altered expression of the TXNIP gene in response to oxLDL treatment compared with controls (<i>p</i> = .04). Following an initial boosting for up to 5 days by inflammatory stimuli, over 14 day, cellular counts [median count ×10⁻⁵ (Q25/Q75)] were utmost in control – [2.6607 (2.0804/4.5357)], followed by cytokine – [0.0433 (0.0026/1.4215)] and significantly lowered in the oxLDL group [0.0274 (0.0023/0.7290); <i>p</i> = .0047]. Osteogenic differentiation [median relative Ca²⁺ content(Q25/Q75)] was significantly lower in cytokine – [0.0066 (0.0052/0.0105)] compared to oxLDL – [0.0144 (0.0108/0.0216)] (<i>p</i> = .0133), with no differences notable for chondrogenic and adipogenic differentiation (<i>p</i> &gt; .05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Within the current investigation's limitations, in contrast to cytokine-mediated inflammation, G-MSCs appear to be minimally responsive to oxLDL-mediated metabolic inflammation, with little negative effect on their differentiation attributes and significantly reduced cellular proliferation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"60 1","pages":"77-89"},"PeriodicalIF":3.4,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jre.13319","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healthy lifestyles and better periodontal health: Results from two large population-based surveys","authors":"Crystal Marruganti,&nbsp;Shailly Luthra,&nbsp;Syed Basit Hussain,&nbsp;Jeanie Suvan,&nbsp;Francesco D'Aiuto","doi":"10.1111/jre.13320","DOIUrl":"10.1111/jre.13320","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To ascertain whether healthy lifestyles are associated with periodontal diseases in two large-scale surveys in the US (National Health and Nutrition Examination Survey – NHANES) and the UK Biobank.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>9854 US adults and 111 679 UK adults were included in the analyses. A healthy lifestyle score (HLS), ranging between 0 and 5, was calculated based on the reported number of healthy behaviours, including never smoking, no heavy alcohol consumption, top third of leisure-time physical activity, higher dietary quality, and ideal sleep duration. The prevalence of periodontal diseases was the primary outcome in both surveys. In the NHANES, periodontal status was assessed through a full-mouth periodontal examination, while in the UKB, only self-reported periodontal status was available.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Multiple regression analyses confirmed that the presence of at least 2–3 healthy behaviours (vs. 0–1) was associated with lower odds of overall and severe periodontitis (ORs 0.5, 0.4–0.6; <i>p</i> &lt; .001 and 0.5, 0.3–0.8; <i>p</i> = .003, respectively) in the NHANES, and of bleeding gums (OR = 0.9, 0.8–1.0; <i>p</i> = .092) and loose teeth (OR = 0.6, 0.5–0.7; <i>p</i> &lt; .001) in UKB. This association increased when considering prevalence of 4–5 healthy behaviours (vs. 0–1) in both the NHANES (periodontitis: OR = 0.3, 0.2–0.4; <i>p</i> &lt; .001; severe periodontitis: OR = 0.1, 0.01–0.2; <i>p</i> &lt; .001) and the UKB (bleeding gums: OR = 0.8, 0.7–0.9; <i>p</i> &lt; .001; loose teeth: OR = 0.5, 0.4–0.6; <i>p</i> &lt; .001). Mediation analyses revealed how these protective associations could be partially mediated (1–14%) by differences in biomarkers of systemic inflammation (white blood cells and neutrophils count as well as C-reactive protein).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Adoption of healthy lifestyle behaviours is associated with a lower prevalence of periodontal diseases within two large population-based samples. This relationship exhibits a dose–response pattern, implying that greater adherence to healthy habits leads to a more significant protective effect against the odds of periodontal diseases. Additionally, our findings suggest that this protective effect is, in part, mediated by reductions in systemic inflammation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"60 2","pages":"144-153"},"PeriodicalIF":3.4,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jre.13320","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}