Effect of low-intensity pulsed ultrasound on the mineralization of force-treated cementoblasts and orthodontically induced inflammatory root resorption via the Lamin A/C-Yes associated protein axis.

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research Pub Date : 2024-08-02 DOI:10.1111/jre.13330

Fu Zheng, Tong Wu, Feifei Wang, Hongyi Tang, Xinyu Cui, Duo Liu, Peng Chen, Jiangfeng Fu, Cuiying Li, Jiuhui Jiang

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引用次数: 0

Abstract

Aims: Orthodontic treatment commonly results in orthodontically induced inflammatory root resorption (OIIRR). This condition arises from excessive orthodontic force, which triggerslocal inflammatory responses and impedes cementoblasts' mineralization capacity. Low-intensity pulsed ultrasound (LIPUS) shows potential in reducing OIIRR. However, the precise mechanisms through which LIPUS reduces OIIRR remain unclear. This study aimed to explore the effects and mechanisms of LIPUS on the mineralization of force-treated cementoblasts and its impact on OIIRR.

Methods: We established a rat OIIRR model and locally administered LIPUS stimulation for 7 and 14 days. We analyzed root resorption volume, osteoclast differentiation, and the expression of osteocalcin and yes-associated protein 1 (YAP1) using micro-computed tomography (micro-CT), hematoxylin and eosin, tartrate-resistant acid phosphatase, immunofluorescence and immunohistochemistry staining. In vitro, we applied compressive force and LIPUS to the immortalized mouse cementoblasts (OCCM30). We assessed mineralization using alkaline phosphatase (ALP) staining, alizarin red staining, real-time quantitative polymerase chain reaction, Western blotting and immunofluorescence staining.

Results: In rats, LIPUS reduced OIIRR, as evidenced by micro-CT analysis and histological staining. In vitro, LIPUS enhanced mineralization of force-treated OCCM30 cells, as indicated by ALP and alizarin red staining, upregulated mRNA expression of mineralization-related genes, and increased protein expression of mineralization markers. Mechanistically, LIPUS activated YAP1 signaling via the cytoskeleton-Lamin A/C pathway, supported by immunofluorescence and Western blot analysis.

Conclusion: This study demonstrates that LIPUS promotes mineralization in force-treated cementoblasts and reduces OIIRR by activating YAP1 through the cytoskeletal-Lamin A/C signaling pathway. These findings provide fresh insights into how LIPUS benefits orthodontic treatment and suggest potential strategies for preventing and treating OIIRR.

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低强度脉冲超声通过 Lamin A/C-Yes 相关蛋白轴对受力处理的骨水泥母细胞矿化和正畸诱导的炎性牙根吸收的影响

目的：正畸治疗通常会导致正畸诱发炎性牙根吸收（OIIRR）。这种情况是由于过度的正畸力引发了局部炎症反应，阻碍了牙骨质母细胞的矿化能力。低强度脉冲超声（LIPUS）显示出减少 OIIRR 的潜力。然而，LIPUS 减少 OIIRR 的确切机制仍不清楚。本研究旨在探讨 LIPUS 对受力处理的骨水泥母细胞矿化的影响和机制及其对 OIIRR 的影响：我们建立了大鼠 OIIRR 模型，并在局部给予 LIPUS 刺激 7 天和 14 天。我们使用显微计算机断层扫描（micro-CT）、苏木精和伊红、耐酒石酸磷酸酶、免疫荧光和免疫组化染色法分析了牙根吸收量、破骨细胞分化以及骨钙素和YAP1（yes-associated protein 1）的表达。在体外，我们对永生化小鼠骨水泥母细胞（OCCM30）施加压缩力和 LIPUS。我们使用碱性磷酸酶（ALP）染色、茜素红染色、实时定量聚合酶链反应、Western印迹和免疫荧光染色来评估矿化情况：在大鼠体内，LIPUS可减少OIIRR，这一点可通过显微CT分析和组织学染色得到证明。在体外，ALP和茜素红染色显示，LIPUS增强了受力处理的OCCM30细胞的矿化作用，上调了矿化相关基因的mRNA表达，并增加了矿化标志物的蛋白表达。从机理上讲，LIPUS通过细胞骨架-层析蛋白A/C通路激活了YAP1信号传导，免疫荧光和Western印迹分析证实了这一点：本研究表明，LIPUS通过细胞骨架-Lamin A/C信号通路激活YAP1，从而促进受力处理的骨水泥母细胞矿化并减少OIIRR。这些发现为了解 LIPUS 如何有益于正畸治疗提供了新的视角，并为预防和治疗 OIIRR 提出了潜在的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of periodontal research 医学-牙科与口腔外科

CiteScore

6.90

自引率

5.70%

发文量

103

审稿时长

6-12 weeks

期刊介绍： The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published. One volume of six issues is published annually.