Journal of periodontal research最新文献

{"title":"Porphyromonas gingivalis Outer Membrane Vesicles Promote Gingival Fibroblasts Senescence via IL21R-AS1.","authors":"Zirui Li, Yijing Xiao, Jiahui Ma, Xu Chen, Yi Zhou, Huiqing Gou, Yan Xu","doi":"10.1111/jre.13419","DOIUrl":"https://doi.org/10.1111/jre.13419","url":null,"abstract":"<p><strong>Aims: </strong>Periodontitis, a chronic inflammatory disease, is associated with accelerated cellular senescence. This study aims to explore how Porphyromonas gingivalis (P. gingivalis) outer membrane vesicles (OMVs) induce senescence and senescence-associated secretory phenotype (SASP) in human gingival fibroblasts (HGFs), focusing on the role of lncRNAs in promoting periodontitis progression.</p><p><strong>Methods: </strong>In vivo, gingival senescence was assessed via immunohistochemistry while bone loss was evaluated using micro-CT, HE and TRAP staining. In vitro, HGFs senescence was validated using CCK-8, SA-β-gal staining, western blot and immunofluorescence assays. IL21R-AS1 was transfected with targeted siRNAs and overexpression plasmids to verify its function. Dual-luciferase reporter assays and rescue experiments were conducted to elucidate the competing endogenous RNA (ceRNA) mechanism of IL21R-AS1. Upstream transcription factors of IL21R-AS1 were validated by CUT&Tag-qPCR and western blot.</p><p><strong>Results: </strong>P. gingivalis OMVs induced gingival tissue senescence and alveolar bone loss in vivo. In vitro, P. gingivalis OMVs promoted HGFs senescence and SASP via IL21R-AS1. IL21R-AS1 acted as a ceRNA by sponging miR-500a-3p, thereby enhancing the regulation of FBXW7. Moreover, P. gingivalis OMVs activated TLR4 and increased PAX5 expression, leading to elevated IL21R-AS1 levels.</p><p><strong>Conclusions: </strong>P. gingivalis OMVs promote HGFs senescence SASP by the upregulation of IL21R-AS1, which acts as a ceRNA, mediating the miR-500a-3p/FBXW7 axis and contributes to the progression of periodontitis. These findings offer novel insights into the molecular mechanisms of OMVs-induced senescence and periodontitis.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Do Patients Prefer in Periodontal and Implant Therapy? A Scoping Review.","authors":"Sarah Gomulinski, Vianney Gandillot, Fabien Valet, Kevimy Agossa, Olivier Huck, Stéphane Kerner, Philippe Bouchard, Maria Clotilde Carra","doi":"10.1111/jre.13412","DOIUrl":"https://doi.org/10.1111/jre.13412","url":null,"abstract":"<p><strong>Aims: </strong>Understanding and integrating patients' preferences into clinical practice can enhance personalized care, improve patient's adherence to treatment, and lead to better therapeutic outcomes. The aim of this scoping review was to map the existing literature investigating patients' preferences in periodontal and implant therapy while identifying key areas for future research and development.</p><p><strong>Methods: </strong>Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses for Scoping Reviews (PRISMA-ScR) guidelines, an electronic search was conducted in four databases (PubMed, Google Scholar, Cochrane Library, and ScienceDirect) in July 2024 to identify studies evaluating patients' preferences for periodontal and implant therapy.</p><p><strong>Results: </strong>The literature search yielded 384 studies, of which eight articles met the inclusion criteria. These studies were conducted between 2003 and 2019 in Brazil, China, Austria, Italy, Germany, Canada, USA, Chile, France, Spain, and Portugal. A total of 1642 patients were included. Preferences were assessed using various quantitative and mixed methodologies. Results indicate a strong preference for treatments aimed at preserving teeth, favoring conservative approaches. When teeth cannot be restored, most patients prefer an implant-supported fixed partial denture to avoid damaging adjacent teeth with a conventional tooth-supported fixed partial denture. In this context, treatment predictability is ranked as the most important factor. While no sociodemographic factors appeared to be associated with preferences in periodontal treatments, several predictors were identified for dental implant therapy. Younger patients, women, individuals with higher education levels, and those with high perceived dental health showed a higher willingness to pay for dental implants.</p><p><strong>Conclusion: </strong>The literature on patients' preferences in periodontal and implant therapies is scarce. Several trends are identified but further longitudinal studies are needed to explore patients' preferences over time and the role of sociodemographic and cultural segmentation criteria.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Faraway, So Close! Patient Desire Is to Keep Teeth!","authors":"Francesco Cairo","doi":"10.1111/jre.13415","DOIUrl":"https://doi.org/10.1111/jre.13415","url":null,"abstract":"","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porphyromonas gingivalis Induces Endothelial Dysfunction Through Sirt3-Dependent CypD Acetylation.","authors":"Shengming Xu, Cheng Zheng, Jianmin Huang, Bin Lu, Hanxin Que, Leyan Xu, Yubo Hou, Linlin He, Xia Fan, Ke Deng, Rongdang Hu, Hui Deng, Yi Wang","doi":"10.1111/jre.13416","DOIUrl":"https://doi.org/10.1111/jre.13416","url":null,"abstract":"<p><strong>Aims: </strong>To investigate how Porphyromonas gingivalis induces endothelial dysfunction, focusing on the regulatory role of Sirtuin 3 (Sirt3) in mitochondrial function.</p><p><strong>Methods: </strong>Differentially expressed Sirtuin family genes in P. gingivalis-infected human aortic endothelial cells (HAECs) were identified through RNA sequencing and validated by quantitative real-time PCR and Western blot. Mitochondrial and endothelial functions were assessed in P. gingivalis-infected HAECs with or without Sirt3-specific agonist Honokiol. Cyclophilin D (CypD) K167 point mutation plasmids were constructed, and Co-immunoprecipitation was performed to investigate the Sirt3-CypD interaction. The vasorelaxation of aortas from mice orally administrated with P. gingivalis was also evaluated.</p><p><strong>Results: </strong>Porphyromonas gingivalis infection in HAECs resulted in mitochondrial and endothelial dysfunction. Mechanistic studies revealed that Sirt3-mediated deacetylation of CypD at K167 was pivotal in alleviating P. gingivalis-induced mitochondrial and endothelial dysfunction. Oral inoculation of P. gingivalis in mice significantly impaired endothelial-dependent vasodilation, disrupted aortic endothelial integrity, increased endothelial cell apoptosis, and elevated mitochondrial reactive oxygen species production. Notably, Sirt3 activation reversed mitochondrial and endothelial dysfunction induced by P. gingivalis both in vivo and in vitro.</p><p><strong>Conclusion: </strong>The present study demonstrated that P. gingivalis induced mitochondrial and endothelial dysfunction, which was mediated through Sirt3-dependent CypD deacetylation.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Non-Surgical Periodontal Therapy on Subgingival Microbiota: A Systematic Review and Meta-Analysis.","authors":"Anna Krajewski, Jeniffer Perussolo, Pinar Ercal, Nikolaos Gkranias, Nikos Donos","doi":"10.1111/jre.13409","DOIUrl":"https://doi.org/10.1111/jre.13409","url":null,"abstract":"<p><strong>Aim: </strong>The objective of this systematic review and meta-analysis was to evaluate the influence of non-surgical periodontal therapy (NSPT) on subgingival microbiota in systemically healthy patients with periodontitis.</p><p><strong>Methods: </strong>A systematic literature search was conducted in Medline, Embase, LILACS, and the Cochrane Library, supplemented by a manual search for studies investigating the effect of NSPT on subgingival microbiota in systemically healthy patients.</p><p><strong>Results: </strong>In this review, 115 studies were included, of which 41 used checkerboard DNA-DNA hybridisation technology, 30 real-time quantitative PCR, 36 bacterial culture, and 8 16S ribosomal ribonucleic acid (rRNA) gene sequencing technology. Meta-analyses showed a significant decrease in mean counts of Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), Treponema denticola (T. denticola), Porphyromonas gingivalis (P. gingivalis), Tannerella forsythia (T. forsythia), Eubacterium nodatum (E. nodatum), Prevotella intermedia (P. intermedia), and Campylobacter rectus (C. rectus) after NSPT. Supportive periodontal care provided a further decrease in mean counts of P. gingivalis and T. denticola at 6 months after NSPT. Qualitative analysis of 16S rRNA gene sequencing studies indicated significant effects of NSPT on the relative abundance of selected disease-and health-associated species and genera, whereas findings on changes in alpha and beta diversity in the subgingival microbiome were heterogeneous.</p><p><strong>Conclusion: </strong>This systematic review showed that NSPT leads to significant reduction of mean abundance of disease-associated microbial species. However, 16S rRNA gene sequencing studies reported heterogeneous findings, which may be associated with methodological aspects. To deepen the understanding of the effect of NSPT on subgingival microbiota, further studies, powered for microbiome changes and with clustered analysis of site-specific samples, are needed.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterials for Reconstructive Treatment of Peri-Implantitis.","authors":"Ausra Ramanauskaite, Anton Sculean, Emilio A Cafferata, Frank Schwarz","doi":"10.1111/jre.13414","DOIUrl":"https://doi.org/10.1111/jre.13414","url":null,"abstract":"<p><p>Peri-implantitis is a pathological condition affecting the tissues surrounding dental implants, marked by inflammation of the peri-implant connective tissue and progressive loss of supporting bone. With strong evidence indicating a bacterial origin, effective treatment approaches should employ anti-infective strategies. Due to the limited success of non-surgical approaches, surgical therapy-offering direct access to contaminated implant surfaces-is often necessary. This narrative review aims to summarize the available biomaterials and their effectiveness in the reconstructive management of peri-implantitis. Reconstructive techniques should be employed in cases with contained defect morphology. While the ideal biomaterial for bone regeneration in these cases remains uncertain, bone substitutes with low resorption rates tend to yield more favorable outcomes compared to autogenous bone. Comparative studies have not demonstrated clear clinical advantages from the adjunctive use of barrier membranes. Current evidence also does not support definitive conclusions regarding the benefits of adding biologics to reconstructive treatments.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic Insights Into the Subgingival Microbiome in Periodontal Health and Different Grades of Periodontitis.","authors":"Yan Zhao, Lu Song, Hao-Yu Li, Shu-Xin Liu, Fei-Fei Mao, Xiao-Jun Li, Pei-Hui Ding","doi":"10.1111/jre.13408","DOIUrl":"https://doi.org/10.1111/jre.13408","url":null,"abstract":"<p><strong>Aim: </strong>This cross-sectional study compared the subgingival microbiome in periodontal health (PH) and periodontitis, focusing on distinguishing Stage III Grade B periodontitis (PD-S3gB) and Stage III Grade C periodontitis (PD-S3gC) as defined by the 2018 Classification of Periodontitis.</p><p><strong>Methods: </strong>Subgingival samples from subjects with PH, PD-S3gB, and PD-S3gC were analyzed using metagenomic sequencing. Taxonomic and functional annotations were performed, followed by analyses of microbial diversity, differential abundance, interspecies networks, predictive modeling, and functional pathway enrichment.</p><p><strong>Results: </strong>Significant differences in both alpha and beta diversity were observed between PH and periodontitis. Several periodontal pathogens were more abundant in disease states, with Capnocytophaga granulosa and Capnocytophaga sp. CM59 enriched in PD-S3gC compared to PD-S3gB. The PD-S3gC group also exhibited a more complex microbial network with increased interspecies connectivity. An 11-species diagnostic model effectively distinguished PH, PD-S3gB, and PD-S3gC. Furthermore, pathways related to motility, chemotaxis, and methane metabolism were significantly enriched in periodontitis.</p><p><strong>Conclusion: </strong>Distinct structural and functional differences in the subgingival microbiome characterize periodontal health and periodontitis. Periodontitis with a rapid rate of progression is marked by specific pathogen overgrowth and enhanced microbial interactions, supporting the development of microbiome-based diagnostics and personalized therapies.</p><p><strong>Trial registration: </strong>Chinese Clinical Trial Registration: ChiCTR2000039426.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Principled Framework for Mendelian Randomization in Oral Health Research.","authors":"Nasir Z Bashir, Mario Romandini, Moritz Kebschull, Konstantinos K Tsilidis, Dominique S Michaud, Stephen Burgess","doi":"10.1111/jre.13411","DOIUrl":"https://doi.org/10.1111/jre.13411","url":null,"abstract":"<p><p>In recent years, the popularity of Mendelian randomization (MR) as a technique to investigate causal relationships has grown exponentially. This approach leverages randomness inherent in the allocation of genetic variants to circumvent issues of unobserved confounding and reverse causation that compromise many causal claims based on observational data. In this manuscript, the key assumptions required for MR analyses to yield credible causal inferences are reviewed. It is emphasized that even technically rigorous MR analyses may yield implausible conclusions if these assumptions are violated. The goal is to provide readers from nonstatistical backgrounds with a clear understanding of the foundational assumptions underpinning MR, complemented by a detailed discussion of specific considerations in oral health research, particularly when periodontitis is used as the exposure or outcome. As with any study design, MR is appropriate and can provide compelling evidence for causality in certain contexts, while its application may be unwarranted in others. This methodological article aims to equip readers with the tools to critically assess MR findings, distinguish scenarios where its use is justified, and understand the key steps for correct execution and reporting. A checklist for assessing the credibility of MR investigations is also provided.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucagon-Like Peptide 1 Receptor Agonists (GLP-1RAs) Improve Periodontal and Peri-Implant Health in Type 2 Diabetes Mellitus.","authors":"Paras Ahmad, Nathan Estrin, Nima Farshidfar, Yufeng Zhang, Richard J Miron","doi":"10.1111/jre.13410","DOIUrl":"https://doi.org/10.1111/jre.13410","url":null,"abstract":"<p><p>Type 2 diabetes mellitus (T2DM) affects millions globally and is strongly associated with oral health issues, particularly periodontitis. The bidirectional relationship between T2DM and periodontitis is well established, with poorly managed T2DM increasing the risk of inflammation, tissue damage, and dental implant failure. Advances in treatment, such as glucagon-like peptide 1 receptor agonists (GLP-1RAs), have led to better glycemic control and may reduce T2DM-related complications, highlighting their potential in addressing interconnected oral-systemic health challenges. This narrative review critically evaluates the literature on the impact of GLP-1RAs on periodontal and peri-implant health. 10 in vitro studies, nine preclinical animal studies, and one clinical study were explored to investigate their effects on periodontal regeneration, implant therapy, and related mechanisms. In vitro research revealed that GLP-1RAs, including exenatide and liraglutide, promoted osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through pathways such as the mitogen Wnt/β-catenin and mitogen-activated protein kinase pathways, even in high glucose or inflammatory conditions. Synergistic effects with stromal cell-derived factor-1 further promoted PDLSC proliferation and bone regeneration. Animal studies demonstrated that GLP-1RAs mitigated periodontal inflammation, oxidative stress, and alveolar bone resorption while promoting bone remodeling and implant osseointegration, independently of glycemic control. Importantly, advanced delivery systems, such as exenatide-loaded chitosan-poly(lactic-coglycolic acid) microspheres, further increased peri-implant osseointegration in diabetic models. The sole clinical study, a retrospective cohort study, assessed peri-implant marginal bone loss in peri-implantitis patients treated with different hypoglycemic drugs. Results showed significantly less clinical and radiographic bone loss in the GLP-1RA group compared to insulin and metformin groups (p < 0.01). Overall, while GLP-1RAs have promising anti-inflammatory, osteoprotective, and pleiotropic properties, their role appears more aligned with preserving periodontal and peri-implant health in T2DM individuals than directly treating periodontitis or peri-implantitis. By delineating current evidence and research directions, this review calls for medical and dental professionals to collaborate in leveraging these novel treatment options in future studies to improve patient care and address the intricate challenges that diabetes presents to oral health.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Submucosal Microbiome in Peri-Implantitis: A Comparative Metagenomic Analysis.","authors":"Lu Song, Wentao He, Hongye Lu, Jimin Jiang, Pei-Hui Ding, Fuming He","doi":"10.1111/jre.13407","DOIUrl":"https://doi.org/10.1111/jre.13407","url":null,"abstract":"<p><p>Through metagenomic sequencing, this study reveals significant microbial and functional differences between peri-implant health and peri-implantitis, identifying pathogenic species, virulence genes, and antibiotic resistance markers that may contribute to disease progression.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}