Journal of periodontal research最新文献

{"title":"Porphyromonas gingivalis-Lipopolysaccharide Induced Gingival Fibroblasts Trained Immunity Sustains Inflammation in Periodontitis.","authors":"Jiayi Liu, Haoyang Tian, Jinhong Ju, Fujiao Nie, Qiuyue Yin, Jingjing Zhao, Suli Wang, Hongmei Guo, Pishan Yang","doi":"10.1111/jre.13372","DOIUrl":"https://doi.org/10.1111/jre.13372","url":null,"abstract":"<p><strong>Aim: </strong>To investigate whether trained immunity occurs in gingival fibroblasts (GFs) and its relationship to the persistence of inflammation in periodontitis.</p><p><strong>Methods: </strong>Periodontally healthy and inflammatory gingival fibroblasts (HGFs and IGFs) were cultured through continuous adherence subculture of tissue blocks. Trained immunity in HGFs was evaluated via a classic in vitro model, with relevant markers assessed via enzyme-linked immunosorbent assay, lactate content assay, glycolytic rate assay, and chromatin immunoprecipitation. A histone methyltransferase blocker and a PI3K inhibitor were added to investigate the mechanisms underlying trained immunity. The relationship between trained immunity and periodontitis was further examined via immunofluorescence staining and chromatin immunoprecipitation on IGFs.</p><p><strong>Results: </strong>Compared with untrained cells, GFs trained with Porphyromonas gingivalis-lipopolysaccharide (P. gingivalis-LPS) exhibited a significant increase in IL-6 and TNF-α secretion, enhanced glycolytic metabolism, and enriched mono-methylation of lysine 4 on histone H3 (H3K4me1) at the enhancer regions of TNF-α and IL-6. The addition of a histone methyltransferase blocker and a PI3K inhibitor greatly reduced trained immunity. Additionally, the response of IGFs to P. gingivalis-LPS stimulation and their epigenetic modifications were similar to those observed in trained HGFs.</p><p><strong>Conclusion: </strong>This study novelly discovered that both P. gingivalis-LPS-stimulated HGFs and IGFs in periodontitis acquired trained immunity. Following P. gingivalis-LPS stimulation, HGFs underwent metabolic and epigenetic changes via the PI3K/AKT pathway, with these epigenetic changes also observed in IGFs. This finding suggests that trained immunity in GFs may be a key mechanism underlying the recurrence and persistence of periodontitis.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontology: The Past, the Present, the Future.","authors":"Jan Lindhe, Mario Romandini","doi":"10.1111/jre.13370","DOIUrl":"https://doi.org/10.1111/jre.13370","url":null,"abstract":"","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Cover of the Journal of Periodontal Research-The Story Behind.","authors":"François Vigouroux, Mario Romandini, Lorenzo Tavelli, Giacomo Baima","doi":"10.1111/jre.13364","DOIUrl":"https://doi.org/10.1111/jre.13364","url":null,"abstract":"","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary Proteomics for Detecting Novel Biomarkers of Periodontitis: A Systematic Review.","authors":"Matteo Corana, Giacomo Baima, Giovanni Iaderosa, Francesco Franco, Jianjian Zhang, Giovanni Nicolao Berta, Federica Romano, Mario Aimetti","doi":"10.1111/jre.13357","DOIUrl":"https://doi.org/10.1111/jre.13357","url":null,"abstract":"<p><strong>Aim: </strong>Salivary content is regarded as a powerful diagnostic window for oral and systemic diseases and the proteomic profile could be useful to distinguish between different periodontal conditions. The aim of the present systematic review was to assess distinctive salivary proteins identified through untargeted proteomics in periodontitis patients compared to periodontally healthy and gingivitis subjects, as well as to provide a qualitative methodological assessment of the current literature.</p><p><strong>Methods: </strong>Relevant studies identified from Medline via PubMed, Scopus, Embase, and Cochrane Library databases were retrieved to answer the following PECO question: \"In systemically healthy individuals, are there any differences in salivary protein expression profiles assessed in proteomics studies between patients with periodontitis and periodontally healthy or gingivitis subjects?\" Moreover, diagnostic utility of the identified markers was sought via a targeted literature search and further quantitative assessment. A modified version of the QUADAOMICS tool was used for the quality assessment of the included studies.</p><p><strong>Results: </strong>After screening 461 relevant articles, a total of 13 studies were selected. The number of identified discriminant salivary proteins ranged from 2 to 4161. However, it was possible to identify proteins that were consistently over- or under-expressed in periodontitis patients in at least 3 studies. Among these, complement C3, profilin-1, SA100A8, and fibrinogen were consistently reported as increased in periodontitis, while cystatin-SN and leukocyte elastase inhibitor were more elevated in periodontally healthy controls. Only 4 studies reported diagnostic accuracy measures, with SA100A8 showing an area under the curve of 0.71 (95% CI: 0.66-0.75) in meta-analysis.</p><p><strong>Conclusions: </strong>Untargeted proteomics techniques identified some key biological molecules which were consistently reported to be over- or under-expressed in periodontitis. These findings could be useful to support novel candidate biomarkers for periodontitis. The high level of heterogeneity in methods and reporting urge to develop standardized protocols to be implemented in this research field (PROSPERO CRD42022299826).</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRPV1 Promotes Periodontitis Tissue Inflammation and Oxidative Damage by Regulating STAT3 Signaling Pathway.","authors":"Mingzhu Yu, Huan Tian, Ruqing Lu, Ni Quan, Ling Qian","doi":"10.1111/jre.13368","DOIUrl":"https://doi.org/10.1111/jre.13368","url":null,"abstract":"<p><strong>Aims: </strong>Periodontitis is a chronic disease affecting adult oral health. Transient receptor potential vanilloid 1 (TRPV1) expression is shown to upregulate in many inflammatory diseases. Nevertheless, its biological potential along with the molecular mechanism in periodontitis is unclear. Our study aimed to explore the biological role and underlying signaling pathway of TRPV1 in periodontitis.</p><p><strong>Methods: </strong>In the current research, human periodontal ligament stem cells (hPDLSCs) were stimulated by lipopolysaccharide (LPS) to induce inflammatory conditions in vitro. In vivo, the periodontitis mouse model was built by ligating the gingival sulcus of male C57BL/6J mice. Thereafter, the proliferation, apoptosis, inflammation, and oxidative stress-related processes were assessed.</p><p><strong>Results: </strong>We found that LPS induced apoptosis and inflammation in hPDLCs, along with oxidative stress, while simultaneously inhibiting hPDLC proliferation (p < 0.05). Notably, TRPV1 expression was elevated in LPS-treated hPDLSCs and gingival samples from patients with periodontitis. Interestingly, the increase in TRPV1 expression induced by Capsaicin, a TRPV1 agonist, inhibited cell proliferation while promoting LPS-stimulated apoptosis, inflammation, and oxidative stress in hPDLSCs (p < 0.01). In contrast, inhibition of TRPV1 expression using Capsazepine, a TRPV1 inhibitor, produced opposite effects (p < 0.01). In vivo experiments revealed that inhibition of TRPV1 attenuated ligation-induced periodontitis in mice, as evidenced by enhanced oxidative stress, inflammatory response, and elevated apoptosis (p < 0.01). Additionally, rescue assays indicated that TRPV1 promoted periodontitis-associated tissue inflammation and oxidative damage via activating the STAT3 signaling pathway (p < 0.01).</p><p><strong>Conclusion: </strong>Our study demonstrates that TRPV1 expression is high in periodontitis and facilitates periodontitis-associated tissue inflammation and oxidative damage by regulating STAT3 signaling pathway, which implies that TRPV1 may represent a new therapeutic target for periodontitis.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repercussions of Long-Term Naproxen Administration on LPS-Induced Periodontitis in Male Mice.","authors":"Jhonatan de Souza Carvalho, Dania Ramadan, Gabriel Garcia de Carvalho, Vinícius de Paiva Gonçalves, Álvaro Formoso Pelegrin, Renata Pires de Assis, Iguatemy Lourenço Brunetti, Marcelo Nicolas Muscara, Denise Madalena Spolidorio, Luís Carlos Spolidorio","doi":"10.1111/jre.13361","DOIUrl":"https://doi.org/10.1111/jre.13361","url":null,"abstract":"<p><strong>Aims: </strong>Chronic periodontitis is the sixth most prevalent disease worldwide and the leading cause of tooth loss in adults. With growing attention on the role of inflammatory and immune responses in its pathogenesis, there is an urgent need to evaluate host-modulatory agents. Non-steroidal anti-inflammatory drugs (NSAIDs) drugs play a crucial role in managing inflammatory conditions. This study examined the repercussions of long-term naproxen use in a periodontal inflammation model known for causing significant inflammation, disrupting epithelial and connective tissue attachment and leading to alveolar bone destruction.</p><p><strong>Methods: </strong>Thirty BALB/c mice were treated with naproxen for 60 days or left untreated. From Day 30, an LPS solution was injected into gingival tissues three times per week for four weeks. This model enables LPS control over the inflammatory stimulus intensity throughout the experimental period, leading to chronic inflammation development involving both innate and adaptive immunity. The liver, stomach and maxillae were submitted to histological analysis. The oxidative damage was determined by measuring lipid peroxidation (LPO) in plasma and gingiva. The activities of myeloperoxidase (MPO), eosinophil peroxidase (EPO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and levels of leukotriene B4, the interleukin (IL)-1β, TNF-α, IL-4, IL-5, IL-10, the chemokine CCL11 were also assessed in the gingival tissues.</p><p><strong>Results: </strong>The results indicated that none of the groups displayed any indications of liver damage or alterations; however, the NPx treatment led to severe gastric damage. In contrast, the treatment alleviated periodontal inflammation, resulting in a reduction of chronic and acute inflammatory cell infiltration and prevention of connective tissue loss in the gingival tissue. Additionally, the treatment increased the activities of endogenous antioxidant enzymes SOD, CAT and GPx, as well as the IL-10 cytokine, while decreasing the levels of leukotriene B4, TNF-α, IL-4 and IL-5. Furthermore, the activities of MPO, EPO and LPO were reduced in the treated groups.</p><p><strong>Conclusion: </strong>These results suggest that NPx effectively inhibits periodontal inflammation in an inflammatory periodontal model. However, the harmful gastric effects dramatically limit its long-term use.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomal miR-155-5p Facilitates Lipopolysaccharide Transport and Foam Cell Formation: A Novel Link Between Periodontitis and Atherosclerosis.","authors":"Wen-Wen Yang, Qing-Xiang Li, Fei Wang, Xin-Ran Zhang, Xian-Li Zhang, Meng Wang, Dong Xue, Ying Zhao, Lu Tang","doi":"10.1111/jre.13369","DOIUrl":"https://doi.org/10.1111/jre.13369","url":null,"abstract":"<p><strong>Aim: </strong>To investigate the role of lipopolysaccharide (LPS) from Porphyromonas gingivalis and miR-155-5p-enriched exosomes in the formation of foam cells and the occurrence of carotid atherosclerosis (CAS).</p><p><strong>Methods: </strong>The CAS tissue samples and plasma from the healthy control group or patients undergoing periodontitis without CAS and with CAS were collected at the Xuanwu Hospital, Capital Medical University. The expression level of miR-155-5p was evaluated by immunofluorescent analysis and qRT-PCR. Oil red O staining and lipid accumulation assays were performed to explore the effects of LPS and miR-155-5p on mouse macrophage Raw264.7 and human monocytes THP-1. The expression levels of lipid-regulated genes were detected by qRT-PCR. Dual-luciferase reporter gene assay and DET1 overexpressed or inhibited Raw264.7 cells were used to verify the target gene of exosomal miR-155-5p. ApoE^-/- mice were used to confirm the auxo-action of atherosclerosis from exosomal miR-155-5p in vivo, and LAL assay was used to detect the LPS content.</p><p><strong>Results: </strong>miR-155-5p was higher in patients with periodontitis and CAS plasma exosomes than those in patients without CAS. The expression of miR-155-5p was significantly increased in CAS tissues compared with Normal tissues, and the expression level of miR-155-5p was associated with lipid-regulated genes in CAS tissues. MiR-155-5p-enriched exosomes accelerated lipid accumulation in macrophage-like cells and promoted the activity of lipid-accumulation genes by targeting DET1. In ApoE^-/- mice, circulating miR-155-5p-enriched exosomes captured LPS, and the LPS-laden exosomes conferred plasma access for LPS, triggering the formation of foam cells and the occurrence of CAS.</p><p><strong>Conclusion: </strong>miR-155-5p enriched exosomes capture and escort LPS to the atherosclerotic sites, licensing the formation of foam cells and thus promoting CAS.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mobile Health to Improve Efficacy of Interdental Brushing Among Periodontitis Patients: A Randomized Clinical Trial.","authors":"Jyoti Yadav, Nishi Tanwar, Rajinder Kumar Sharma, Aditi Sangwan","doi":"10.1111/jre.13362","DOIUrl":"https://doi.org/10.1111/jre.13362","url":null,"abstract":"<p><strong>Aim: </strong>Compliance with plaque control measures in open interdental spaces, an essential element in management of periodontitis, is reported to be poor. Mobile health (mHealth) approach is an effective approach for behavior change. This study aimed to assess the effectiveness of mHealth in improving plaque control in type 2 embrasures.</p><p><strong>Methods: </strong>Stage II or III, grade B periodontitis patients (n = 76) with type 2 embrasures were enrolled. Phase I therapy was provided in both groups. In the control group, interdental brushing instructions were given only at baseline and follow-up appointments. In the test group, interdental brushing was also reinforced by regular text messages for 3 months. Clinical parameters including plaque index (PI), papillary bleeding index (PBI), probing pocket depth (PPD), clinical attachment level (CAL), gingival index (GI), bleeding on probing (BOP), and interdental papillary height (IPH) were measured at baseline, 1, 3, and 6 months.</p><p><strong>Results: </strong>Intergroup comparison from baseline to 6 months showed a statistically significant difference (p < 0.05) between groups in all parameters with more reduction in mean values in test group. Intergroup comparison from 3 to 6 months showed a statistically significant difference (p < 0.05) in mean values of PI, PBI, GI, PPD, CAL, and IPH with higher reduction in test group. Intragroup comparison from 3 to 6 months showed statistically significant reduction in mean values in PI, PBI, PPD, CAL, and IPH only in test group (p < 0.05).</p><p><strong>Conclusion: </strong>Cue to action through mobile text messages effectively augments compliance with the use of interdental brush, improves plaque control, and manages stage II or stage III periodontitis following subgingival instrumentation.</p><p><strong>Trial registration: </strong>ClinicalTrials.gov identifier: NCT05565404.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative Potential of PDL-Derived Small Extracellular Vesicles.","authors":"Anahid A Birjandi, Steven Lynham, Eva Matalova, Mandeep Ghuman, Paul Sharpe","doi":"10.1111/jre.13356","DOIUrl":"10.1111/jre.13356","url":null,"abstract":"<p><p></p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperglycemia Exacerbates Periodontal Destruction via Systemic Suppression of Regulatory T Cell Number and Function.","authors":"Masami Saotome, Ryutaro Kuraji, Yukihiro Numabe","doi":"10.1111/jre.13366","DOIUrl":"https://doi.org/10.1111/jre.13366","url":null,"abstract":"<p><strong>Aim: </strong>Diabetes is a significant risk factor that exacerbates the pathological progression of periodontal disease. In recent years, attention has focused on the effect of regulatory T cells (Tregs), which play a central role in immune tolerance, on inflammatory processes in periodontal tissue, suggesting a link with diabetes-associated periodontitis. In this study, we examined the dynamics of Tregs in periodontal tissue of mice with streptozotocin (STZ)-induced hyperglycemia.</p><p><strong>Methods: </strong>Eleven-week-old male C57BL/6J mice were divided into four treatment groups: Untreated (C group), ligature placed around the maxillary second molars with silk sutures (PD group), intraperitoneal administration of STZ (HG group), and ligature placed after STZ administration (PHG group). Establishment of hyperglycemia was assessed 14 days after STZ administration, and ligation was performed 7 days later. After another 7 days of ligation, the mice were euthanized. The right side of the maxilla was observed histopathologically, whereas the palatal gingiva on the left side of the maxilla was analyzed genetically, and the microstructure of the alveolar bone was also assessed. In addition, lymphocytes from peripheral blood, spleen, and periodontal tissue were analyzed using flow cytometry.</p><p><strong>Results: </strong>In bone structure analyses, alveolar bone height, bone volume/tissue volume (BV/TV), and bone mineral density (BMD) were lower in the PHG group than the PD group. In the gingival tissue, expression of the Foxp3 gene was up-regulated in the PHG group compared with the C group, and IL-17a was up-regulated in the PHG group compared with the PD group. Flow cytometry analyses showed that the number of Tregs (CD4⁺CD25⁺Foxp3⁺ cells) in the blood and gingival tissue was significantly higher in the PD and PHG groups than the C group. The number of CD4⁺CD25^-Foxp3⁺ cells, which are reportedly functionally attenuated as Tregs, was increased in blood of the PHG group. Immunofluorescence staining of periodontal tissue showed that the number of CD25⁺Foxp3⁺ cells was significantly increased only in the PD group, whereas a trend toward an increased number of CD25^-Foxp3⁺ cells was observed in the PHG group.</p><p><strong>Conclusion: </strong>The present study showed that STZ-induced hyperglycemia numerically and functionally attenuates Tregs in a mouse model of experimental periodontitis. Furthermore, impaired immune tolerance capacity appears to be involved in exacerbating inflammation and bone destruction in periodontal tissue.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}