Journal of periodontal research最新文献

{"title":"Can We Combine Mouthrinses With Probiotics? An Evaluation of Their Compatibility and Combined Therapy on Oral Biofilms.","authors":"Katalina Lauwens, Mehraveh Saghi, Pieter-Jan Germonpré, Naiera Zayed, Nico Boon, Kristel Bernaerts, Wim Teughels, Wannes Van Holm","doi":"10.1111/jre.70033","DOIUrl":"https://doi.org/10.1111/jre.70033","url":null,"abstract":"<p><strong>Aim: </strong>Multiple oral pathologies requiring antiseptic mouthrinses for prevention or treatment. However, nonselective elimination of the microbes may also harm beneficial commensal, healthy bacteria. Promicrobial strategies, such as probiotics, aim to rebalance the oral microbiome rather than eradicate it; however, we hypothesised that their incorporation might be challenged due to the microbiome's inherent resistance to outsiders. In this study, their combined effect on an in vitro oral biofilm model was assessed. Instead of comparing mouthrinses to probiotics, could they be combined to obtain the best of both worlds?</p><p><strong>Methods: </strong>The compatibility of two commonly used Limosilactobacillus reuteri strains with 13 commercially available mouthrinses was tested by evaluating probiotic survival in the mouthrinses and their combined effect on a multispecies in vitro biofilm model.</p><p><strong>Results: </strong>Differences in compatibility were observed. Several mouthrinses showed moderate compatibility and improved the probiotics incorporation into biofilms. One mouthrinse (O7 Active Oxygen) demonstrated the most favourable compatibility, enabling abundant probiotic incorporation and beneficial biofilm composition changes.</p><p><strong>Conclusion: </strong>The combination of mouthrinses and probiotics has the potential to improve the treatment of oral pathologies and promote a healthier oral microbiome, although compatibility varies.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics Comparison of Proline-Rich Peptide-Enhanced Hyaluronic Acid Gels Versus Conventional Regenerative Materials: An Early Wound-Healing Model.","authors":"Øystein Øvrebø, Ståle Petter Lyngstadaas, Thaqif El Khassawna, Reem Jamous, Qianli Ma, Fernando Muñoz, Maria Permuy, Antonio Gonzalez Cantalapiedra, Antonio José Serrano-Muñoz, Joana Maria Ramis, Marta Monjo, Filippo Rossi, Håvard J Haugen","doi":"10.1111/jre.70032","DOIUrl":"https://doi.org/10.1111/jre.70032","url":null,"abstract":"<p><strong>Aims: </strong>To compare the early wound-healing responses to crosslinked hyaluronic acid enriched with two proline-rich peptides (P2, P6) against unmodified hyaluronic acid and the enamel-matrix derivative (EMD) in a porcine gingival-detachment model.</p><p><strong>Methods: </strong>In six pigs, defects around premolars were treated with HA, HA + P2, HA + P6 or EMD. After 6 days, the sites were harvested and evaluated using histology, immunohistochemistry, multiplex cytokine assay and untargeted proteomics of the gels, which were examined, informing an integrated multiomics approach analysis.</p><p><strong>Results: </strong>Both peptide formulations improved the composite histological score compared to HA alone, and HA + P6 matched EMD in suppressing oedema, TNF-α staining and CD80⁺ macrophages while increasing mannose-receptor labelling. Proteomics revealed that P2 upregulated actin (Q6QAQ1) and histone H2A (F2Z5L5), consistent with active remodelling, whereas EMD reduced trypsin and trypsinogen, indicating a more stabilised wound milieu; HA + P6 showed an intermediate yet favourable profile. IL-6 levels were lower in both peptide groups and EMD than in HA, and all gels proved noncytotoxic, with HA + P6 enhancing cell viability.</p><p><strong>Conclusion: </strong>HA + P6 suppressed TNF-α and trypsin signalling while boosting M2-linked markers and proresolution proteins to the same extent as EMD, whereas plain HA did not. Thus, adding a short proline-rich peptide confers EMD-like early immunomodulation on a fully synthetic HA carrier. These observations, although limited to 6-day soft tissue healing, warrant longer studies in true periodontal regeneration models.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regeneration or Repair? Evaluating Foreign Material in Periodontal Healing.","authors":"Giovan Paolo Pini Prato, Riccardo Di Gianfilippo","doi":"10.1111/jre.70035","DOIUrl":"https://doi.org/10.1111/jre.70035","url":null,"abstract":"","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gestational Diabetes Shapes Gingival Crevicular lncRNA Expression in Early Pregnancy: A Prospective Cohort Study.","authors":"Alejandra Chaparro, María José Bendek, María Luisa Mizgier, Valeria Ramírez, Gino Nardocci, Sebastián E Illanes","doi":"10.1111/jre.70027","DOIUrl":"https://doi.org/10.1111/jre.70027","url":null,"abstract":"<p><p>This study identifies a transcriptomic profile of long noncoding RNAs in gingival crevicular fluid samples in pregnant women with gestational diabetes risk. NEAT1 and LINC-PINT were increased expression in gingival crevicular fluid samples in pregnancies later diagnosed with gestational diabetes mellitus.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osseous Resective Surgery: The Past, the Present and the Future","authors":"Aimetti Mario,&nbsp;Carnevale Gianfranco","doi":"10.1111/jre.70029","DOIUrl":"10.1111/jre.70029","url":null,"abstract":"&lt;p&gt;The main goal of periodontal therapy is to arrest the destructive progression of disease and preserve natural dentition. The resolution of inflammation, the reduction of pocket depth (PD), and the stabilization or improvement of clinical attachment level (CAL) are the core objectives of treatment. These therapeutic outcomes are typically pursued through a stepwise approach beginning with risk factor control and non-surgical therapy (Step I and II) [&lt;span&gt;1&lt;/span&gt;]. Ideally, these early interventions lead to a clinical scenario characterized by probing depths of ≤ 4 mm, a biologically favorable condition that enables long-term periodontal stability [&lt;span&gt;2, 3&lt;/span&gt;]. However, in many patients, pockets ≥ 4 mm with bleeding on probing persist despite comprehensive non-surgical therapy [&lt;span&gt;4, 5&lt;/span&gt;]. These sites represent an ongoing risk for disease progression and typically warrant surgical intervention to achieve a more stable periodontal environment [&lt;span&gt;6&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;Periodontal therapy offers three distinct histological outcomes: repair, most commonly characterized by the formation of a long junctional epithelium; regeneration, involving the restoration of periodontal ligament, cementum, and alveolar bone; and what might be termed a “reset”—the reestablishment of a physiologic situation with a short junctional epithelium and a minimal probing depth, achieved through apical positioning of the marginal periodontal tissues [&lt;span&gt;7&lt;/span&gt;]. Each of these outcomes carries different biological and clinical implications, and the chosen surgical strategy should take into account the patient-specific risk profile, reflect the anatomy of the defect, and aim to achieve predictable long-term therapeutic outcomes.&lt;/p&gt;&lt;p&gt;Among available surgical modalities, flap surgery with osseous resection (ORS) remains a well-established and predictable technique, specifically aimed at eliminating periodontal pockets, reestablishing a “correct” and maintainable anatomical architecture, and preserving or increasing an adequate band of attached gingiva [&lt;span&gt;8, 9&lt;/span&gt;]. Despite the concept—supported by controlled clinical trials—that long-term outcomes of different periodontal surgical procedures may converge, ORS continues to represent a highly effective and predictable clinical tool. Its goal is not only to eliminate pockets but also to correct soft and hard tissue deformities, thereby restoring a functional dento-alveolar relationship conducive to long-term stability. When properly indicated and meticulously executed, ORS contributes meaningfully to periodontal health and remains one of the principal surgical strategies with documented long-term success. Importantly, ORS achieves the anatomical, histological, and physiological outcome of a healthy periodontium albeit positioned at a more apical level. This deliberate architectural reset creates a new, stable marginal profile that facilitates effective plaque control and reduces the risk of disease rec","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"60 8","pages":"743-747"},"PeriodicalIF":3.4,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jre.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Risk Indicators of Peri-Implant Diseases and Buccal Soft-Tissue Dehiscence: A Cross-Sectional Study From a University-Based Cohort.","authors":"Giacomo Baima, Federica Romano, Sompol Chuachamsai, Marta Ciccarelli, Andrea Lo Giudice, Marco Ventricelli, Giulia Maria Mariani, Mario Romandini, Gianmario Schierano, Mario Aimetti","doi":"10.1111/jre.70025","DOIUrl":"https://doi.org/10.1111/jre.70025","url":null,"abstract":"<p><strong>Aim: </strong>To assess the prevalence of peri-implant diseases and buccal peri-implant soft-tissue dehiscence (PISTD) and to identify the associated risk indicators.</p><p><strong>Methods: </strong>Patients previously rehabilitated with implant-supported rehabilitations at the University of Turin Dental School were specifically recalled with a registry-based approach for this cross-sectional study. Data collection included medical and dental history, full-mouth clinical examination, and periapical radiographs. Moderate/severe peri-implantitis was diagnosed based on bone loss (direct criterion, when available) or bone level (indirect criterion) ≥ 2 mm and the presence of bleeding/suppuration. PISTD was defined as mucosal dehiscence on the buccal aspect of at least one implant site. Multilevel, multivariable logistic regression models were applied to identify factors associated with moderate/severe peri-implantitis and buccal PISTD.</p><p><strong>Results: </strong>Of the 397 patients contacted, 146 were included (mean age 61.1 ± 14.5 years; current smokers 34.3%; stage III-IV periodontitis 65.1%) with a total of 511 dental implants (mean function time: 13.3 years [2-31]). Implant survival rate was 96.5%. Moderate/severe peri-implantitis was detected in 56.8% of patients and 34.7% of implants. Prevalence of buccal PISTD was 54.1% and 40.5%, respectively. Protective indicators for moderate/severe peri-implantitis included supportive peri-implant care > twice a year (OR = 0.16; 95% CI: 0.03-0.95), > 2 mm of keratinized tissue height (OR = 0.44; 95% CI: 0.21-0.95), and correct mesio-distal implant positioning (OR = 0.54; 95% CI: 0.32-0.94). Risk indicators included stage III-IV periodontitis (OR = 2.82; 95% CI: 1.30-6.15), function time ≥ 10 years (OR = 3.02; 95% CI: 1.55-5.89), bisphosphonate use during follow-up (OR = 5.96; 95% CI: 1.33-26.66), and presence of a cantilever (OR = 5.51; 95% CI: 1.56-19.38). For PISTD, protective indicators were mandibular location (OR = 0.45; 95% CI: 0.25-0.81), thick buccal soft-tissue phenotype (OR = 0.18; 95% CI: 0.08-0.42), and > 2 mm of keratinized tissue height (OR = 0.05; 95% CI: 0.02-0.15). Risk indicators included peri-implantitis (OR = 2.21; 95% CI: 1.25-3.91), use of intermediate abutments (OR = 4.92; 95% CI: 1.92-12.58), and proximity to adjacent implants (OR = 3.35; 95% CI: 1.50-7.48) or edentulous spaces (OR = 3.38; 95% CI: 1.51-7.54).</p><p><strong>Conclusion: </strong>In this long-term, university-based cohort, peri-implant diseases and PISTD were highly prevalent. Multiple patient- and implant-level factors emerged as significant risk or protective indicators. Despite the widespread occurrence of peri-implant diseases, long-term implant survival remained high, challenging current diagnostic thresholds and underscoring the need for refined, progression-based definitions.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thirty Years of Autologous Platelet Concentrates: From Platelet-Rich Plasma to Platelet-Rich Fibrin.","authors":"Richard J Miron, Nathan E Estrin, Paras Ahmad, Nima Farshidfar, Masako Fujioka-Kobayashi, Yufeng Zhang, Mario Romandini, Reinhard Gruber","doi":"10.1111/jre.70013","DOIUrl":"https://doi.org/10.1111/jre.70013","url":null,"abstract":"<p><p>Nearly three decades have now passed since autologous platelet concentrates (APCs) were introduced into clinical practice. Harnessing the body's innate intrinsic capacity for healing, the concepts of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) emerged to concentrate growth factors and cells at supra-physiological levels. Nearly three decades ago, Professor Robert Marx demonstrated that PRP-obtained via centrifugation of whole blood with anti-coagulants-could accelerate healing across a wide range of indications in both dentistry and medicine. Subsequent research showed that eliminating anti-coagulants, leading to the development of PRF, resulted in an even more natural and faster healing process. In dentistry, APCs have been consistently shown to accelerate healing of both soft and hard tissues, either as standalone treatments or in combination with other biomaterials. PRF, in particular, has demonstrated benefits within several dental applications, spanning from root coverage, periodontal regeneration of intra-bony and furcation defects, ridge preservation, third molar extraction healing, sinus grafting, guided bone regeneration (GBR), and peri-implant soft tissue healing. Beyond dentistry, PRF has also proven effective in various medical indications-improving outcomes in the treatment of diabetic ulcers, managing joint pain and cartilage degradation in sports medicine, and enhancing outcomes in esthetic medicine. This review highlights the evolution of APCs, with a focus on their clinical applications and recent technological advancements. These include horizontal centrifugation techniques for PRF and more refined protocols for producing concentrated PRF (C-PRF). Lastly, the article introduces extended-PRF (e-PRF) membranes, created through heat-induced albumin denaturation, which prolong membrane degradation from the typical 2-3 weeks to up to 4 months. These innovative e-PRF membranes, composed of albumin gel and liquid PRF (Alb-PRF), are now being used as alternatives to collagen membranes in routine dental procedures.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herpesvirus Simplex Virus-1 Exploits Inflammation to Infect Periodontal Stem Cells and Disrupt Lineage Commitment.","authors":"Araceli Valverde, Raza Ali Naqvi, Yinghua Chen, Alireza Moshaverinia, Anne George, Deepak Shukla, Gloria Martinez, Gabriella Chapa, Salvador Nares, Afsar R Naqvi","doi":"10.1111/jre.70022","DOIUrl":"https://doi.org/10.1111/jre.70022","url":null,"abstract":"<p><strong>Aims: </strong>Elevated levels of Herpes Simplex Virus 1 (HSV-1) have been reported in periodontitis, however, the tropism and relationship with periodontal inflammation are poorly characterized. This study investigated how inflammation affects viral tropism toward human periodontal ligament stem cells (hPDLSCs).</p><p><strong>Methods: </strong>HSV-1 gB and gD transcripts in healthy and diseased human gingiva were measured by RT-qPCR and confirmed in HSV-1-infected murine gingiva. HSV-1 infection in hPDLSCs was analyzed by imaging and flow cytometry. hPDLSCs were individually treated with IL-6, TNF-α, GMCSF, IL-10, or PgLPS, and HSV-1 replication was assessed by infecting with the 17 GFP strain. Lineage markers in virally infected hPDLSCs during osteogenic differentiation were measured by RT-qPCR and immunofluorescence in vitro and validated in vivo. Mice subjected to ligature-induced periodontitis (LIP) and infected with HSV-1 were examined for gingival histology, inflammatory cytokines, and alveolar bone loss.</p><p><strong>Results: </strong>Inflamed human gingiva showed higher expression of viral transcripts compared to healthy controls. In mouse oral HSV-1 infection, gB and gD expression increased over time, with higher levels in mice with ligature-induced periodontitis. Virus infected hPDLSCs challenged with inflammatory mediators or PgLPS showed higher GFP, while IL-10 treatment attenuated GFP levels. Importantly, HSV-1 17 GFP infection affected osteoblast lineage commitment by promoting the expression of key transcription factors in vitro and in vivo. Compared to the LIP alone group, higher levels of inflammatory markers and bone loss were evident in HSV-1 infected with LIP.</p><p><strong>Conclusion: </strong>hPDLSCs are trophic to HSV-1 in vitro and in vivo, with periodontal inflammation playing a significant role in viral tropism.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soft-Tissue Phenotype Modification as an Adjunct to the Treatment of Peri-Implant Mucositis-A Quasi-Randomized Clinical Trial.","authors":"Zeynep Aksu, Sila Cagri Isler, Berrin Unsal, Andrea Roccuzzo, Mario Romandini","doi":"10.1111/jre.70014","DOIUrl":"https://doi.org/10.1111/jre.70014","url":null,"abstract":"<p><strong>Aim: </strong>This quasi-randomized clinical trial evaluated the additional benefit of soft-tissue phenotype modification via free palatal graft (FPG) as an adjunct to non-surgical therapy for peri-implant mucositis. A secondary objective was to determine whether any observed effects were mediated by improved plaque control.</p><p><strong>Methods: </strong>Forty-three patients (55 implants) with peri-implant mucositis and keratinized tissue width (KTW) < 2 mm were enrolled. One month after oral hygiene instructions and non-surgical treatment, participants were quasi-randomly allocated to either an FPG procedure (test group, n = 22) or no additional intervention (control group, n = 21). The primary outcome was bleeding on probing (BoP) extent at 6 months (i.e., number of bleeding sites per implant). Secondary outcomes included peri-implant phenotype parameters (KTW, tissue thickness, and vestibular depth), plaque extent, and other peri-implant health measures (BoP severity, probing pocket depth [PPD], peri-implant soft-tissue dehiscence [PISTD], and treatment success). Intergroup comparisons were performed using regression analyses, and a mediation analysis assessed whether treatment effects were mediated by improved plaque control.</p><p><strong>Results: </strong>At 6 months, FPG significantly increased KTW (mean difference [MD] = 2.36 mm; p < 0.001) and tissue thickness (MD = 0.97 mm; p < 0.001), while reducing plaque extent (MD = -1.49; p < 0.001), compared with the control group. BoP extent was significantly lower in the test group (0.75 ± 1.07) than in controls (1.83 ± 1.20) (MD = -1.06; 95% CI: -1.67 to -0.44; p = 0.001). Additionally, the test group exhibited lower BoP severity (MD = -0.50; p < 0.001) and higher treatment success (OR = 8.44; p = 0.001). No significant differences were observed in PPD and PISTD. Mediation analysis suggested that the observed benefits of FPG on peri-implant health were largely independent of improved plaque control.</p><p><strong>Conclusion: </strong>FPG effectively modified the peri-implant soft-tissue phenotype and, as an adjunct to non-surgical therapy, provided additional benefits in managing peri-implant mucositis. The effects on peri-implant health were not attributable to improved plaque control.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limosilactobacillus reuteri-Fusobacterium nucleatum Interactions Modulate Biofilm Composition and Immunogenicity.","authors":"Luming Yang, Gopu Sriram, Ren Jie Jacob Chew, Kai Soo Tan","doi":"10.1111/jre.70021","DOIUrl":"https://doi.org/10.1111/jre.70021","url":null,"abstract":"<p><strong>Aim: </strong>The interactions between Limosilactobacillus reuteri and oral bacteria are poorly understood. This study seeks to characterize how two strains of L. reuteri coaggregate with Fusobacterium nucleatum, determining the impact on the biofilm composition and immunogenicity.</p><p><strong>Methods: </strong>A series of in vitro experiments was conducted using L. reuteri DSM 17938 and ATCC PTA 5289, Fusobacterium nucleatum ATCC 25586, and Porphyromonas gingivalis W50. The coaggregation between individual strains of L. reuteri, F. nucleatum, and P. gingivalis was evaluated using the tube coaggregation assay and confocal microscopy. Biofilm compositions were determined by confocal microscopy and culture. The effect of coaggregation on the immunogenicity of L. reuteri-F. nucleatum aggregates were evaluated using periodontal ligament fibroblasts, oral epithelial cells, and monocytes.</p><p><strong>Results: </strong>Both L. reuteri DSM and PTA strains demonstrated coaggregation with F. nucleatum. This interaction reduced the amount of F. nucleatum in biofilm by 1000-fold. Additionally, the coaggregation between L. reuteri and F. nucleatum lowered its immunogenicity. Furthermore, the coaggregation of L. reuteri with F. nucleatum led to a 50% reduction in the amount of P. gingivalis present in the biofilm.</p><p><strong>Conclusion: </strong>This study demonstrates novel mechanisms through which L. reuteri can exert its effects as a probiotic. The coaggregation with L. reuteri modulates the immunogenicity of F. nucleatum and impairs its ability to serve as the bridging species, altering the biofilm composition, thus limiting the extent of dysbiosis.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}