Journal of periodontal research最新文献

{"title":"Severe Periodontitis is Associated With Recurrent Cardiovascular Events-A 10-Year Longitudinal Cohort Study.","authors":"Stefan Reichert, Selina Rehm, Axel Schlitt, Susanne Schulz","doi":"10.1111/jre.13365","DOIUrl":"https://doi.org/10.1111/jre.13365","url":null,"abstract":"<p><strong>Aim: </strong>The study aimed to elucidate a putative association between severe periodontitis and the incidence of recurrent cardiovascular events in patients with cardiovascular disease (CVD) within 10 years after their initial hospitalisation.</p><p><strong>Methods: </strong>A cohort of 1002 stationary patients with angiographically proven CVD was included. They were examined regarding prevalence of severe periodontitis (≥ 30% of the teeth with proximal attachment loss of ≥ 5 mm), probing depth, clinical attachment loss, bleeding on probing, number of missing teeth and oral care habits. Recurrent events were summarised as combined end point (myocardial infarction, stroke/transitory ischemic attack, cardiovascular death and death caused by stroke). Survival analyses were carried out after a 10-year follow-up period. Hazard ratios (HRs) were adjusted for known cardiac risk factors using Cox regression.</p><p><strong>Results: </strong>The follow-up was completed by 792 patients. The overall incidence of the combined end point was 42.8%. Severe periodontitis was associated with recurrent cardiovascular events (adjusted hazard ratio [HR] = 1.26, 95% confidence interval [CI] 1.0-1.58 and Standard error [SE] 0.11), whereas both, tooth brushing more than once a day (adjusted HR = 0.74, 95% CI 0.57-0.97, SE 0.13) and performing interdental hygiene (adjusted HR = 0.71, 95% CI 0.52-0.99, SE 0.16) decreased this risk.</p><p><strong>Conclusions: </strong>Severe periodontitis is a putative risk factor for recurrent cardiovascular events.</p><p><strong>Trial registration: </strong>ClinicalTrials.gov identifier: NCT01045070.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesaconate from Bacillus subtilis R0179 Supernatant Attenuates Periodontitis by Inhibiting Porphyromonas gingivalis in Mice.","authors":"Weiwei Zhao, Lingli Ji, Jie Li, Dandan Liu, Changqing Yan, Chenying Zhang, Xiaozhe Wang, Yang Liu, Shuguo Zheng","doi":"10.1111/jre.13363","DOIUrl":"10.1111/jre.13363","url":null,"abstract":"<p><strong>Aims: </strong>This research sought to assess the efficacy of Bacillus subtilis (B. subtilis) R0179 and explore potential metabolites in mitigating experimental periodontitis in mice induced by Porphyromonas gingivalis (P. gingivalis) ATCC 33277.</p><p><strong>Methods: </strong>B. subtilis R0179 was administered to 8-week-old male C57BL/6J mice with periodontitis. Oral load of P. gingivalis ATCC 33277 and periodontal tissue loss were quantified. The cell-free supernatant (CFS) was separated to assess its anti-P. gingivalis effect. Proteomic and metabolomic analyses identified potential antibacterial components in the CFS, further evaluated for anti-P. gingivalis effects.</p><p><strong>Results: </strong>B. subtilis R0179 significantly reduced P. gingivalis ATCC 33277 levels and mitigated periodontal tissue loss in mice. The CFS, rather than inactivated B. subtilis R0179 cells, exhibited antibacterial activity. Proteomic and metabolomic analyses identified mesaconate and citraconate as key antibacterial agents. Disk diffusion assays confirmed the efficacy of mesaconate against P. gingivalis, while citraconate had no effect. Mesaconate showed a dose-dependent reduction in P. gingivalis ATCC 33277 population and periodontal tissue loss in mice.</p><p><strong>Conclusion: </strong>These findings highlight B. subtilis R0179 and its metabolite mesaconate as promising candidates for therapeutic development against periodontitis by inhibiting P. gingivalis ATCC 33277 effectively.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Particulate Matter 2.5 and Severe Periodontitis Among Colombian Adults.","authors":"Meisser Madera, Johana Márquez-Lázaro, Eduardo Bernabe","doi":"10.1111/jre.13360","DOIUrl":"https://doi.org/10.1111/jre.13360","url":null,"abstract":"<p><strong>Aims: </strong>The primary aim was to determine the association between particulate matter 2.5 (PM_2.5) concentration at municipality-level and severe periodontitis among adults. A second aim was to evaluate contemporaneous versus lagged effects of exposure to municipality-level PM_2.5 concentration on severe periodontitis.</p><p><strong>Methods: </strong>We linked individual-level data from the latest National Oral Health Survey (ENSAB-IV) with satellite-based estimates of annual PM_2.5 concentrations at the surface level for municipalities in Colombia. Annual PM_2.5 concentrations were averaged over 3, 5 and 10  years to capture contemporaneous and lagged effects, respectively. Severe periodontitis was defined using three common case definitions. The association between municipality-level PM_2.5 concentration and severe periodontitis was tested in multilevel logistic regression models adjusting for covariates.</p><p><strong>Results: </strong>Data from 9111 adults in 197 municipalities and 33 departments were analyzed. The prevalence of severe periodontitis varied from 10.4% to 29.8% depending on the case definition used. The mean PM_2.5 concentration was 18.5 (SD = 2.9), 19.1 (3.0) and 18.9 (2.8) μg/m³ over the past 3, 5 and 10 years. The municipality-level PM_2.5 concentration was not associated with severe periodontitis, irrespective of the assessment period for PM_2.5 concentration (3, 5, or 10  years) or the case definition of periodontitis used.</p><p><strong>Conclusions: </strong>This study found no association between municipality-level PM_2.5 concentration and severe periodontitis among Colombian adults. No evidence of lagged effects was found either.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment Tools for Masticatory Function in Periodontitis Patients: A Scoping Review.","authors":"Chris T C Fok, Aneesha Acharya, Bonnie H M Chin, Melissa Rachel Fok, George Pelekos","doi":"10.1111/jre.13359","DOIUrl":"https://doi.org/10.1111/jre.13359","url":null,"abstract":"<p><strong>Aims: </strong>The aim of this scoping review was to map the available evidence on assessment tools for masticatory function for periodontitis patients. It also aimed to examine the methodology of masticatory function assessment and to identify the elements of subjective masticatory function evaluation for periodontitis patients reported in the literature.</p><p><strong>Methods: </strong>A scoping review was conducted following the methodological guidance for the conduct of scoping reviews. Embase, MEDLINE, Web of Science, and Scopus were systematically searched for published studies in English reporting objective or subjective masticatory function assessment in periodontitis patients.</p><p><strong>Results: </strong>Forty-five studies were included in the analysis. The identified assessment tools for masticatory function were summarized using the terminology described by the recent consensus. Heterogeneity was observed in the approach of assessment, the type(s) and design of assessment tools, and the methods of measurement employed. Most studies utilized only one assessment tool. Seven studies reported composite objective assessment and five studies utilized assessment tools for both objective and subjective masticatory function. Items from the included instruments for subjective masticatory function were analyzed and categorized into seven potentially clinically relevant elements of subjective masticatory function evaluation. Unclear reporting on validation status was found in all included instruments for subjective masticatory function.</p><p><strong>Conclusion: </strong>Variable methodologies have been reported to assess masticatory function in periodontitis patients. Future research is needed to discern the clinical utility of these assessment tools for masticatory function in periodontitis patients.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plexin-B2 Mediates Orthodontic Tension-Induced Osteogenesis via the RhoA/F-Actin/YAP Pathway.","authors":"Qiming Li, Xinyi Chen, Xinyi Li, Xiaoge Jiang, Xingjian Li, Xinrui Men, Yan Li, Song Chen","doi":"10.1111/jre.13358","DOIUrl":"https://doi.org/10.1111/jre.13358","url":null,"abstract":"<p><strong>Aims: </strong>This study aims to investigate the role of Plexin-B2 in tension-induced osteogenesis of periodontal ligament stem cells (PDLSCs) and its biomechanical mechanism.</p><p><strong>Methods: </strong>In vitro, cyclic tension simulated orthodontic forces to assess Plexin-B2 expression in PDLSCs. We then knocked out Plexin-B2 using lentivirus to explore its role in tension-induced osteogenesis. In vivo, we used nickel-titanium springs to establish orthodontic tooth movement (OTM) models in mice. Local periodontal Plexin-B2 expression was knocked down using adeno-associated viruses (AAVs) to study its influence on new bone formation under mechanical tension in OTM models. Molecular mechanisms were elucidated by manipulating Plexin-B2 and RhoA expression, assessing related proteins, and observing F-actin and Yes-associated protein (YAP) through immunofluorescence.</p><p><strong>Results: </strong>Plexin-B2 expression in PDLSCs increased under cyclic tension. Decrease of Plexin-B2 reduced the expression of osteogenic protein in PDLSCs and negatively affected new bone formation during OTM. RhoA expression and phosphorylation of ROCK2/LIMK2/Cofilin decreased in Plexin-B2 knockout PDLSCs but were reversed by RhoA overexpression. The level of F-actin decreased in Plexin-B2 knockout PDLSCs but increased after RhoA rescue. Nuclear YAP was reduced in Plexin-B2 knockout PDLSCs but increased after RhoA overexpression.</p><p><strong>Conclusions: </strong>Plexin-B2 is involved in tension-induced osteogenesis. Mechanistically, the RhoA signaling pathway, the F-actin arrangement, and the nuclear translocation of YAP are involved in the mechanotransduction of Plexin-B2.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preclinical Evaluation of a Poly (Lactic Acid/Caprolactone) Bilayer Membrane and a Carbonate Apatite for Periodontal Regeneration: A Canine One-Wall Intrabony Defect Model.","authors":"Daichi Yamaki, Shunsuke Fukuba, Munehiro Okada, Shunsuke Takeuchi, Shu Hoshi, Patrick R Schmidlin, Takanori Iwata","doi":"10.1111/jre.13355","DOIUrl":"https://doi.org/10.1111/jre.13355","url":null,"abstract":"<p><strong>Aims: </strong>This preclinical study aimed to evaluate the periodontal tissue regenerative capacities of poly (lactic acid/caprolactone) (PLCL) bilayer membrane in one-wall intrabony defects in dogs. No study has assessed the efficacy of PLCL bilayer membrane for periodontal regeneration therapy despite the fact that PLCL bilayer membrane has proved efficient for bone regeneration.</p><p><strong>Methods: </strong>In five beagle dogs, the bilateral mandibular second and fourth premolars were extracted 8 weeks before the experimental surgery. Standardized bone defects (5 mm in height and 6 mm in width) were surgically created on the mesial and distal roots of the bilateral third premolars in the mandible. The test groups were set up as follows: (i) carbonate apatite (CO₃Ap) + PLCL, (ii) CO₃Ap, (iii) xenograft (DBBM) + collagen membrane (CM), and (iv) DBBM. The control group was left empty. Radiological, histologic and histomorphometric characteristics were compared 8 weeks after surgery.</p><p><strong>Results: </strong>No infectious complications were detected at any of the tested sites. The test groups exhibited a greater height and volume of the newly formed bone than the control group. They also showed a greater height of the newly formed cementum than the control group. However, the results were not statistically significant. The newly formed periodontal ligaments were inserted into newly formed bone and cementum in the test groups.</p><p><strong>Conclusion: </strong>The combined use of PLCL bilayer membrane and CO₃Ap demonstrated comparable performance for periodontal tissue regeneration in one-wall intrabony defects compared to conventional therapies.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of different extraction indications on the morphological changes in the maxillary sinus: A retrospective cohort study.","authors":"Meng Wang, Fei-Fei Mao, Xiao-Hui Jin, Jia-Ping Huang, Anna Dai, Pei-Hui Ding","doi":"10.1111/jre.13348","DOIUrl":"https://doi.org/10.1111/jre.13348","url":null,"abstract":"<p><strong>Aims: </strong>The comprehensive effects of maxillary posterior tooth extraction on the maxillary sinus (MS) morphology remain to be thoroughly elucidated. This retrospective cohort study aimed at evaluating the influence of different extraction indications on the morphological changes in the MS by utilizing cone-beam computed tomography (CBCT).</p><p><strong>Methods: </strong>One hundred and seventy-eight of maxillary posterior tooth extractions underwent CBCT scans before and after extraction using 3D Slicer software. Parameters such as maxillary sinus pneumatization (MSP, the primary outcome measure), buccal bone height (BBH), palatal bone height (PBH), mucosal thickness (MT), and other anatomical structures were measured for patients undergoing extraction due to periodontitis, periapical lesions, or tooth fracture. Multiple linear regression analysis was employed to assess the effect of extraction indications on the MS.</p><p><strong>Results: </strong>While the primary outcome, MSP, did not reveal statistically significant differences across various indications for tooth extraction (p > .05), extraction itself resulted in MSP (p < .05). The rate of this pneumatization was influenced by the position of the extraction site (p < .05). Additionally, baseline values of bone height and mucosal thickness showed an inverse correlation with the rate of change in these parameters following tooth extraction (p < .001).</p><p><strong>Conclusions: </strong>Tooth extraction led to increased pneumatization of the maxillary sinus while simultaneously reducing bone height and mucosal thickness. However, these outcomes were not influenced by the reason for tooth extraction.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Implant Therapy in Patients With and Without a History of Periodontitis: A Systematic Review With Meta-Analysis of Prospective Cohort Studies.","authors":"Marco Annunziata, Gennaro Cecoro, Agostino Guida, Gaetano Isola, Paolo Pesce, Roberto Sorrentino, Massimo Del Fabbro, Luigi Guida","doi":"10.1111/jre.13351","DOIUrl":"https://doi.org/10.1111/jre.13351","url":null,"abstract":"<p><strong>Aim: </strong>This systematic review investigates the effectiveness of implant therapy in patients with and without a history of periodontitis in terms of implant loss, peri-implant marginal bone loss (MBL), and occurrence of peri-implant diseases.</p><p><strong>Methods: </strong>The protocol of the present meta-analysis was registered on PROSPERO (CRD42021264980). An electronic search was conducted up to April 2024. All prospective cohort studies reporting implant loss, MBL, and occurrence of peri-implant diseases in both patients with a history of periodontitis (HP) and patients with no history of periodontitis (NHP) after at least 36-month follow-up were included. The risk of bias was evaluated using the Newcastle-Ottawa Scale and the quality of the evidence was also assessed. A meta-analysis was performed on the selected outcomes at the available follow-up time points. Subgroup analyses were conducted based on follow-up time, rate of progression and severity of periodontitis, and implant surface characteristics. Publication bias was evaluated using the Funnel plot and Egger's test.</p><p><strong>Results: </strong>From 13 761 initial records, 14 studies (17 articles) were finally included. Eight studies had a low risk of bias level, and six had a medium risk of bias level. Meta-analysis showed that HP patients had a significantly greater risk for implant loss (HR: 1.75; 95% CI: 1.28-2.40; p = 0.0005; I² = 0%), MBL (MD: 0.41 mm; 95% CI 0.19, 0.63; p = 0.0002; I² = 54%), and peri-implantitis (3.24; 95% CI: 1.58-6.64; p = 0.001; I² = 57%) compared to NHP, whereas no significant intergroup difference for peri-implant mucositis was found. Subgroup analyses revealed a particularly greater risk for implant loss for HP patients over a ≥ 10-year follow-up (HR: 2.02; 95% CI: 1.06-3.85; p = 0.03; I² = 0%) and for patients with a history of grade C (formerly aggressive) periodontitis (HR: 6.16; 95% CI: 2.53-15.01; p < 0.0001; I² = 0%). A greater risk for implant loss for stages III-IV (severe) periodontitis, and implants with rough surfaces was also found.</p><p><strong>Conclusions: </strong>Within the limits of heterogeneous case definitions and methods of assessment, a history of periodontitis has been proved to significantly increase the risk for implant loss, particularly at long follow-up (≥ 10 years) and in case of rapidly progressive forms (grade C), and for MBL and peri-implantitis.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Micro-CT and Morphometric Outcomes in a Modified Experimental Rat Model of Periodontitis.","authors":"Jaime Bueno, María Martínez, Nagore Ambrosio, Juan Del Rosal, Javier Robledo-Montaña, Juan D Gamonal, Leire Virto, Eduardo Montero, David Martín-Hernández, María José Marín, David Herrera, Mariano Sanz, Borja García-Bueno, Juan C Leza, Håvard J Haugen, Elena Figuero","doi":"10.1111/jre.13354","DOIUrl":"https://doi.org/10.1111/jre.13354","url":null,"abstract":"<p><strong>Aim: </strong>To assess the correlation between micro-computed tomography (micro-CT) and linear morphometric measurements in terms of mandibular bone levels in a modified experimental periodontitis model in rodents to study the mechanisms of association between periodontal destruction and neuroinflammation.</p><p><strong>Methods: </strong>The proposed in vivo experimental periodontitis model involves the administration of oral rinses with Porphyromonas gingivalis and Fusobacterium nucleatum, four times per week during 4, 8 or 12 weeks, in 24 male Wistar Hannover rats (180 g, 5 weeks old). After euthanasia, hemi-mandibles were collected. One hemi-mandible was analysed using morphometry, while the other was assessed with micro-CT. Linear measurements were taken at the buccal aspect and furcation level for both techniques, and volumetric measurements were also obtained with micro-CT. Passing-Bablok regression analysis was used to compare the results of both techniques, with morphometric measurements serving as the reference. Moreover, Lin's Concordance correlation coefficient was calculated to assess the level of agreement. Periodontal clinical variables with neuroinflammatory parameters from the frontal cortex were used to evaluate the association between the resulting condition and neuroinflammation.</p><p><strong>Results: </strong>Twenty-one out of the initial 24 rats were analysed. The micro-CT linear measurements demonstrated high concordance values with the linear morphometric measurements at the buccal surfaces of the roots in molars (r = 0.714) but not at the furcation area (r = 0.052). At 12 weeks, there was a significant impact on neuroinflammation with significant decreases in iNOS levels and p-mTOR levels at 4 and 8 weeks.</p><p><strong>Conclusion: </strong>The proposed in vivo experimental periodontitis model demonstrated a high degree of correlation between morphometric and micro-CT measurements in buccal areas but not at the furcation level. Concomitantly, there was a significant temporary modulation of the neuroinflammatory response.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Analysis of Porphyromonas gingivalis Lipopolysaccharide-Induced Early Gene Expression in Human Gingival Keratinocytes.","authors":"Mahyar Ostadkarampour, Edward E Putnins","doi":"10.1111/jre.13353","DOIUrl":"https://doi.org/10.1111/jre.13353","url":null,"abstract":"<p><strong>Aim: </strong>Porphyromonas gingivalis lipopolysaccharide (PgLPS) is a significant virulence factor and a driver of early innate immune responses in epithelial cells. The presence of PgLPS in immediate proximity to gingival epithelium induces significant inflammatory responses. In primary human gingival keratinocytes (HGK), we utilized transcriptome analysis to elucidate the change in early gene expression induced by PgLPS.</p><p><strong>Methods: </strong>HGK cell cultures were treated with PgLPS (4 h), and RNA was extracted and prepared for RNA sequence (RNAseq) analysis. Differentially expressed genes (DEGs) were identified, and potential interactions between these genes were subsequently examined using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analytic approaches to identify significantly enriched pathways. Expression of genes associated with relevant pathways was evaluated using real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR).</p><p><strong>Results: </strong>RNAseq analysis identified 25 DEGs, and GO and KEGG analytic approaches showed related genes expressed in two general pathways. First, pathways broadly related to urokinase and coagulation included the genes PLAU, PLAUR, and SerpinB2. In RT-qPCR analysis, these genes were induced by PgLPS over time (4-24 h), and these data were consistent with PgLPS induction of cell migration. Second, interleukin-1 (IL-1) receptor binding and cytokine-activity pathways were also enriched. Genes associated with these pathways included IL36G, IL1B, IL1RN, and CXCL14. RT-qPCR analysis confirmed PgLPS induction of genes associated with the IL-1family. When expression of IL1B and IL36G genes was examined in relation to their respective antagonists, only IL36G gene expression was increased. CXCL14 gene expression was reduced over time, and this was consistent with RNAseq analysis.</p><p><strong>Conclusions: </strong>Genes associated with significantly enriched GO and KEGG pathways are relevant to aspects of periodontal disease (PDD) pathogenesis. First, PgLPS induced expression of PLAU, PLAUR, and SerpinB2, and these changes were consistent with an increase in cell migration that was found. Second, both IL36G and IL1B gene expression was significantly induced, but only IL36G in relation to its selective antagonist (IL36RN) was increased. These data support that early upregulation of IL36G may serve as an alarmin that can drive early innate immune inflammatory responses in HGK. Further in vivo testing of these findings is ongoing.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}