Journal of periodontal research最新文献

{"title":"Submucosal Microbiome in Peri-Implantitis: A Comparative Metagenomic Analysis.","authors":"Lu Song, Wentao He, Hongye Lu, Jimin Jiang, Pei-Hui Ding, Fuming He","doi":"10.1111/jre.13407","DOIUrl":"https://doi.org/10.1111/jre.13407","url":null,"abstract":"<p><p>Through metagenomic sequencing, this study reveals significant microbial and functional differences between peri-implant health and peri-implantitis, identifying pathogenic species, virulence genes, and antibiotic resistance markers that may contribute to disease progression.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk of Bias Evaluation of Cross-Sectional Studies: Adaptation of the Newcastle-Ottawa Scale.","authors":"Maria Clotilde Carra, Pierluigi Romandini, Mario Romandini","doi":"10.1111/jre.13405","DOIUrl":"https://doi.org/10.1111/jre.13405","url":null,"abstract":"<p><p>Cross-sectional studies are widely utilized in medical research to estimate prevalence and examine associations. As such, they can serve as a significant source of data for systematic reviews. However, specific considerations are necessary when evaluating the risk of bias (RoB) of cross-sectional studies, as several potential biases can undermine the validity, reliability, and robustness of their findings. This article introduces a novel, context-specific tool designed to assess the RoB of cross-sectional studies for use in systematic reviews. The proposed tool represents an adaptation of the Newcastle-Ottawa Scale (NOS), originally developed for cohort and case-control studies. Similar to the original NOS, the new tool (named \"NOS-xs\") features a nine-star rating system to assess six specific items across three main domains: (i) study sample selection, (ii) assessment of exposure(s) and outcome(s), and (iii) confounding factors. Based on the number of awarded stars, studies are categorized as having high (0-3 stars), moderate (4-6 stars), or low (7-9 stars) RoB. The NOS-xs tool maintains consistency with the original NOS tool, facilitating its integration into systematic reviews that also include cohort and/or case-control studies. While the NOS-xs is suited to analytical cross-sectional studies (i.e., association studies), a simplified version (\"NOS-xs2\") is also introduced for descriptive cross-sectional studies (i.e., prevalence studies). The NOS-xs2 features a four-star rating system to assess three of the six specific items included in the NOS-xs. To streamline their application, spreadsheets for both NOS-xs and NOS-x2 are provided.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-223-3p Mitigates Mitochondrial Dysfunction and Cementoblast Apoptosis in Orthodontic Root Resorption via FoxO3.","authors":"Wei He, Lei Xu, Wei Jiang, Song Yao, Yu Fu, Zishuo Cheng, Danlan Zhang, Lan Huang","doi":"10.1111/jre.13384","DOIUrl":"https://doi.org/10.1111/jre.13384","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study was to elucidate the roles of miR-223-3p in orthodontically induced inflammatory root resorption (OIIRR).</p><p><strong>Methods: </strong>We used high-throughput miRNA sequencing and transcriptome sequencing to analyze the differentially expressed miRNAs and mRNAs in OCCM-30 cells under hypoxia. Real-time quantitative PCR (RT-qPCR) and Western blotting were used to assess the expression of genes and proteins related to apoptosis, oxidative stress, and mitochondrial dysfunction. Fluorescence staining was employed to detect changes in cellular ROS (reactive oxygen species), MMP (mitochondrial membrane potential), and mtROS (mitochondrial ROS) expression.</p><p><strong>Results: </strong>We found that miR-223-3p targeted FoxO3 to regulate apoptosis in cementoblasts under hypoxic conditions. Moreover, hypoxia-induced FoxO3 increased oxidative stress and induced mitochondrial dysfunction in cementoblasts, resulting in cell apoptosis. Administration of the ROS inhibitor NAC (N-acetyl cysteine) effectively reversed FoxO3-induced oxidative stress and mitochondrial dysfunction, thereby rescuing cell apoptosis.</p><p><strong>Conclusions: </strong>miR-223-3p targets FoxO3 and regulates the apoptosis of cementoblasts by improving oxidative stress and mitochondrial dysfunction. These findings may offer new insights into the mechanism of OIIRR.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal Clinical Practice: The Past, the Present, the Future","authors":"Myron Nevins","doi":"10.1111/jre.13392","DOIUrl":"https://doi.org/10.1111/jre.13392","url":null,"abstract":"","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"60 3","pages":"203-205"},"PeriodicalIF":3.4,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographic Influence on Subgingival Microbiota in Health and Periodontitis: A Multinational Shotgun Metagenomic Study.","authors":"Arredondo Alexandre, Àlvarez Gerard, Isabal Sergio, Teughels Whim, Laleman Isabelle, Contreras Maria José, Isbej Lorena, Huapaya Enrique, Mendoza-Azpur Gerardo, Mor Carolina, Nart José, Blanc Vanessa, León Rubén","doi":"10.1111/jre.13406","DOIUrl":"https://doi.org/10.1111/jre.13406","url":null,"abstract":"<p><strong>Aims: </strong>To assess the differences in the taxonomical and functional profile of the subgingival microbiota isolated from healthy subjects (HS) and patients with periodontitis (PP) from four different countries.</p><p><strong>Methods: </strong>In this study, 80 subgingival samples from HS and PP from four different countries (Belgium, Chile, Peru, and Spain) were analyzed using shotgun metagenomic sequencing.</p><p><strong>Results: </strong>The results indicated significant variation in α-diversity between HS and PP, segregated by country, with PP from Peru clearly standing out from the rest. In terms of composition, β-diversity was explained more by the country of origin (6.8%) than by the diagnosis (4.1%). In addition, more than 75 different taxa, 63 of which were identified at the species level, showed significantly different relative abundances when comparing the country of origin, diagnosis, and both variables combined. Moreover, 85 metabolic pathways showed significantly different relative abundances between HS and PP, with species commonly associated with periodontitis, such as Porphyromonas gingivalis and Tannerella forsythia, strongly contributing to the reinforcement of periodontitis-associated pathways. On the other hand, differences in functional profiles based on the country of origin were almost nonexistent, suggesting that variability in taxonomic profiles does not have a direct impact on healthy or periodontitis-associated functional profiles.</p><p><strong>Conclusion: </strong>These findings suggest that microbial analysis should take into account the geographic origin of samples in order to provide a more accurate description of the subgingival microbiota. Moreover, they lay the groundwork for larger and more comprehensive studies that might analyze this phenomenon in the future.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annual Incidence and Pre-Treatment Prognostic Factors of Labial Gingival Recessions in Young Orthodontically Treated Patients.","authors":"Gualtiero Mandelli, Alice Mandelli, Gianluigi Fiorillo, Giorgio Gastaldi, Lucia Medoro, Marco Clementini","doi":"10.1111/jre.13403","DOIUrl":"https://doi.org/10.1111/jre.13403","url":null,"abstract":"<p><p>Gingival recession occurrence is highly prevalent after orthodontic treatment, and keeratinised tissue width at baseline is significantly correlated with the prevalence, extension, and severity of gingival recession, clearly demonstrating the importance of evaluating periodontal factors in planning orthodontic treatment.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Connective Tissue Grafts in the Regenerative Treatment of Intrabony Defects: A Systematic Review and Meta-Regression Analysis.","authors":"Kevimy Agossa, Hamoun Sabri, Dumitru Chele, Javier Calatrava, Michel Bravard, Hom-Lay Wang","doi":"10.1111/jre.13402","DOIUrl":"https://doi.org/10.1111/jre.13402","url":null,"abstract":"<p><strong>Aim: </strong>To assess the effect of connective tissue graft (CTG) in the treatment of periodontal intrabony defects (IDs), focusing on changes in postoperative gingival recession (GR) depth and regenerative outcomes.</p><p><strong>Methods: </strong>A systematic search was conducted across MEDLINE-PubMed, EMBASE, Cochrane CENTRAL, and Google Scholar for articles published through December 31, 2024. Randomized controlled trials (RCTs) comparing treatment outcomes in IDs treated with or without CTG were included in a meta-regression analysis. A mixed-effect linear regression model was employed to estimate the effect of CTG on postoperative GR depth, probing depth (PPD) reduction, clinical attachment level (CAL) gain, and bone fill (BF).</p><p><strong>Results: </strong>Twenty-three studies were selected, with five RCTs (176 IDs) included in the meta-regression. Of these, two RCTs compared bone graft (BG) + CTG to BG + resorbable membrane (MB), one compared periosteal vs. nonperiosteal CTG combined with BG, one compared open flap debridement (OFD) + CTG to OFD alone, and one compared BG + CTG to either OFD or MB. The use of CTG was significantly associated with a reduction in GR (mean effect size of 0.981 mm, 95% CI: 0.573 to 1.389, p = 0.001), PPD (mean effect size of 1.160 mm, 95% CI: 0.318 to 2.002, p = 0.010), as well as improvements in CAL (mean effect size of 1.105 mm, 95% CI: 0.420 to 1.790, p = 0.004) and BF (mean effect size of 1.382 mm, 95% CI: 0.595 to 2.169, p = 0.002).</p><p><strong>Conclusion: </strong>Within the limitations of the study, the use of CTG in periodontal regenerative therapy for IDs appears beneficial in reducing postoperative GR and might further enhance regenerative outcomes compared to treatments without CTG.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accuracy of Metabolomics in Peri-Implant Crevicular Fluid for Diagnosis and Prognosis of Peri-Implantitis.","authors":"Hatem Alassy, Elias Kersten, Janelle Hamilton, Barbara Botorous, Angelomaria Iuorio, Todd Rappe, Massimo Costalonga","doi":"10.1111/jre.13400","DOIUrl":"https://doi.org/10.1111/jre.13400","url":null,"abstract":"<p><strong>Aims: </strong>Poor accuracy of diagnostic and prognostic tools prevents the prediction of peri-implant disease stability or progression. We analyzed metabolites from peri-implant crevicular fluid (PICF) samples from healthy and diseased implants to identify those diagnostic of health and peri-implant disease and predictive of peri-implant bone loss over time.</p><p><strong>Methods: </strong>Clinical, radiographic examinations and PICF samples were collected from 59 healthy implants, 33 implants with peri-implantitis, and 38 implants with peri-implant mucositis in 71 subjects. A subset of implants was evaluated at 6, 12, 18, and 24 months. Over time, all initially healthy implants remained stable (Group B, N = 28), whereas 6 initially diseased implants continued to lose bone and 8 did not (Group C). PICF metabolites were measured using proton-nuclear magnetic resonance (1H-NMR) 2-dimensional Total Correlation Spectroscopy. PCA and PLS-DA tested the cross-sectional clustering and importance of each metabolite, while the AUC summarized the accuracy of predicting radiographic bone changes ≥ 1 mm at 6-month intervals.</p><p><strong>Results: </strong>At baseline, the Cadaverine/Lysine and Putrescine/Lysine signatures diagnosed peri-implantitis (AUC = 0.76 and 0.70; p < 0.000) with good accuracy, while alpha-ketoglutarate diagnosed implant health (AUC = 0.706; p = 0.002). Combining metabolites increased diagnostic accuracy (AUC_{Cadaverine/Lysine+Methionine} = 0.81; p < 0.01). Proline and 1-3-diaminopropane predicted future bone loss (AUC_Proline = 0.917 and AUC_{1-3-diaminopropane} = 0.854). ANOVA post hoc analysis established that biotin and propionate levels were higher in Group C compared to Groups A and B (p < 0.001; AUC_biotin = 0.889; AUC_propionate = 0.87). Valine levels were higher in Groups A and C compared to Group B (p = 0.002; AUC = 0.841).</p><p><strong>Conclusions: </strong>¹H-NMR 2-dimensional spectroscopy identified PICF metabolites diagnostic of peri-implantitis with high accuracy. Despite the small number of affected implants, metabolite signatures that predict future bone loss in peri-implantitis appear to be different from those diagnostic of peri-implantitis.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enamel Matrix Derivative in the Reconstructive Surgical Therapy of Peri-Implantitis: A Randomized Clinical Trial.","authors":"Erik Regidor, Carlotta Dionigi, Martina Ghoraishi, Jon Salazar, Anna Trullenque-Eriksson, Jan Derks, Alberto Ortiz-Vigón","doi":"10.1111/jre.13396","DOIUrl":"https://doi.org/10.1111/jre.13396","url":null,"abstract":"<p><strong>Aims: </strong>To evaluate the adjunctive effect of enamel matrix derivative (EMD) in the reconstructive surgical therapy of peri-implantitis.</p><p><strong>Methods: </strong>Forty subjects (44 implants) affected by peri-implantitis (PPD ≥ 5 mm, positive BOP/SOP, intraosseous defect with a depth of ≥ 3 mm and width of ≤ 4 mm) were randomly allocated to one of two surgical protocols: control (access flap + bone graft + resorbable membrane) or test (access flap + bone graft + resorbable membrane + EMD). Clinical outcomes (PPD, BOP, SOP, buccal REC, and buccal KM) were assessed at baseline, 6 and 12 months after surgery. Radiographic marginal bone levels (MBL) and patient-reported outcomes (PROs) were recorded at baseline and at 12 months. Post-operative complications and Early Healing Index (EHI) scores were recorded at 2 weeks. The primary outcome was PPD change. Two composite outcomes (CO) were assessed: CO1 was defined as \"implant not lost, PPD ≤ 5 mm, REC ≤ 1 mm and complete absence of BOP/SOP\"; CO2 defined as CO1 but allowing for 1 site with BOP.</p><p><strong>Results: </strong>Four patients (four implants) were lost to follow-up. At 12 months, no implants were lost, 73% of implants presented with PPD ≤ 5 mm (control: 65.2%; test: 81.0%; p = 0.316) and PPD reduction amounted to 4.0 ± 1.7 and 4.3 ± 2.4 mm in control and test groups, respectively (p = 0.105). No significant differences in terms of clinical, radiographic, composite and PROs were observed between groups. No significant differences in EHI scores were found at 2 weeks between groups.</p><p><strong>Conclusion: </strong>This study failed to demonstrate any benefit of the adjunctive use of EMD in the reconstructive surgical therapy of peri-implantitis.</p><p><strong>Trial registration: </strong>ISRCTN18159776 (https://doi.org/10.1186/ISRCTN18159776).</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Selcopintide on Periodontal Tissue Regeneration in Chronic One-Wall Intrabony Defect Model.","authors":"Young-Chang Ko, Dong-Seol Lee, Yang-Jo Seol, Yong-Moo Lee, Ki-Tae Koo, Joo-Cheol Park, Won-Jun Shon, Jungwon Lee","doi":"10.1111/jre.13395","DOIUrl":"https://doi.org/10.1111/jre.13395","url":null,"abstract":"<p><strong>Aims: </strong>Periodontal diseases pose significant challenges to oral health, making the regeneration of periodontal tissues a critical therapeutic goal. The goal is to restore dental function by repairing damaged tissue and reconstructing the healthy connective structure between the teeth and the alveolar bone. This study aimed to investigate the effects of selcopintide (SCPT) on the differentiation of periodontal ligament cells (PDLCs), cementoblasts, and osteoblasts in vitro, as well as the regeneration of periodontal tissue using a periodontal tissue defect model in dogs in vivo.</p><p><strong>Methods: </strong>The expression of periodontal tissue marker genes, including periostin (POSTN), cementum attachment protein (CAP), dentin matrix protein 1 (DMP1), and bone sialoprotein (BSP), was investigated in vitro. Chronic one-wall intrabony defects were created in a total of 12 beagle dogs (n = 6 at 6 and 12 weeks, respectively), and the surgical sites were treated with no treatment, guided tissue regeneration (GTR), GTR with SCPT 50 μg/0.1 mL, 100 μg/0.1 mL, and 250 μg/0.1 mL. The effects of SCPT on the regeneration of periodontal tissues, such as periodontal ligament (PDL), cementum, and bone, were analyzed in vivo.</p><p><strong>Results: </strong>SCPT influenced the proliferation and differentiation of cementoblasts and PDLCs. Real-time polymerase chain reaction analysis showed that SCPT upregulated the expression of POSTN, CAP, DMP1, and BSP compared to the control. In the periodontal defect model, SCPT regenerated the periodontal complex. Additionally, the arrangement of the newly formed PDL-like fibers was perpendicular to the newly formed cementum and alveolar bone, similar to Sharpey's fibers in natural teeth, compared with the control.</p><p><strong>Conclusion: </strong>In this preclinical study, histological and immunohistochemical analyses suggest that GTR with SCPT might be associated with increased periodontal ligament attachment and enhanced cementum and alveolar bone formation. Additional research with a larger sample size is needed to establish the optimal therapeutic protocols and validate the regenerative potential of SCPT.</p>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}