Journal of NeuroVirology最新文献_第4页

Unveiling the impact of simulated microgravity on HSV-1 infection, neuroinflammation, and endogenous retroviral activation in SH-SY5Y cells. 揭示模拟微重力对HSV-1感染、神经炎症和SH-SY5Y细胞内源性逆转录病毒激活的影响。

IF 2.3 4区医学

Journal of NeuroVirology Pub Date : 2025-04-01 Epub Date: 2025-03-20 DOI: 10.1007/s13365-025-01251-0

Seyedesomaye Jasemi, Elena Rita Simula, Kawaguchi Yasushi, Leonardo Antonio Sechi

{"title":"Unveiling the impact of simulated microgravity on HSV-1 infection, neuroinflammation, and endogenous retroviral activation in SH-SY5Y cells.","authors":"Seyedesomaye Jasemi, Elena Rita Simula, Kawaguchi Yasushi, Leonardo Antonio Sechi","doi":"10.1007/s13365-025-01251-0","DOIUrl":"10.1007/s13365-025-01251-0","url":null,"abstract":"Microgravity (µg) during spaceflight affects cellular and molecular functions of both human cells and microbial pathogens, influencing viral replication and the host immune system. This study aimed to investigate the effects of simulated µg on Herpes Simplex Virus-1 (HSV-1) replication, host pro-inflammatory cytokine, and human endogenous retrovirus (HERV) activation in human neuroblastoma SH-SY5Y cells. Our results show that µg has a negative impact on HSV-1 replication, leading to significantly reduced viral titers and lower expression levels of HSV-1 early genes (ICP0, ICP4, and ICP27) compared to 1 gravity (1 g) conditions. Interestingly, despite lower viral titers and HSV-1 gene expressions under µg condition, we observed higher levels of HERVs and pro-inflammatory cytokine gene expression. In addition, there was a significant correlation between HSV-1 immediate-early genes with HERVs and pro-inflammatory cytokine gene expression, with stronger correlations observed under µg conditions. Taken together, µg reduces HSV-1 replication and increases host pro-inflammatory and HERVs gene expression, which demands further investigation for human health protection in space.","PeriodicalId":16665,"journal":{"name":"Journal of NeuroVirology","volume":" ","pages":"154-162"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progressive multifocal leukoencephalopathy during 4 years of Palbociclib for advanced breast cancer with a history of follicular lymphoma patient. 4年帕博西尼治疗晚期乳腺癌伴滤泡性淋巴瘤患者进展性多灶性脑白质病

IF 2.3 4区医学

Journal of NeuroVirology Pub Date : 2025-04-01 Epub Date: 2025-04-21 DOI: 10.1007/s13365-025-01255-w

Ryosuke Koyamada, Mariko Nishihara, Tetsuhiro Masaki, Tomohito Shimada, Kazuhiro Ishikawa, Jun Hashimoto, Nobuyoshi Mori, Asami Namai, Hideaki Yokoo, Kenta Takahashi, Tadaki Suzuki, Kazuo Nakamichi, Shinichiro Mori

引用次数: 0

SARS-CoV-2: A synergy to the Alzheimer's disease. SARS-CoV-2：与阿尔茨海默病的协同作用

IF 2.3 4区医学

Journal of NeuroVirology Pub Date : 2025-02-01 Epub Date: 2025-02-25 DOI: 10.1007/s13365-025-01247-w

Khaja Moinuddin Shaik, Deepak Kumar, Pirangi Srikanth, Sukhendu Nandi

{"title":"SARS-CoV-2: A synergy to the Alzheimer's disease.","authors":"Khaja Moinuddin Shaik, Deepak Kumar, Pirangi Srikanth, Sukhendu Nandi","doi":"10.1007/s13365-025-01247-w","DOIUrl":"10.1007/s13365-025-01247-w","url":null,"abstract":"COVID-19 was a nightmare in humankind's history that challenged our advanced medical technology. All credit goes to the researchers who played a crucial role in curbing COVID-19 and proved our medical technology supremacy. However, COVID-19 has left some mysterious scars on human well-being. It is believed that COVID-19 has a significant negative impact on various cardiovascular (CVS) and central nervous system (CNS) diseases, especially in the case of CNS diseases like Alzheimer's. Surprisingly, COVID-19 affects the respiratory system, whereas Alzheimer's disease (AD) alters brain function. To explain this phenomenon, several hypotheses were proposed, but the mechanism needs to be clearly understood. Another critical thing to be concerned about is that COVID-19 will worsen pre-existing conditions and lead to the onset of AD. In the race to curb COVID-19, the invention of vaccines was speeded up, and it is necessary to fight against COVID-19. However, postvaccination follow-up is mandatory when an individual is a victim of AD. In this review article, we compiled the various dreadful effects of the COVID-19 virus on AD, the Post effects of the virus on AD, and the effect of the COVID-19 vaccination on AD. This article provides a new direction for research concerning COVID-19 and AD.","PeriodicalId":16665,"journal":{"name":"Journal of NeuroVirology","volume":" ","pages":"16-23"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Momordica charantia phytoconstituents can inhibit human T-lymphotropic virus type-1 (HTLV-1) infectivity in vitro and in vivo. 苦瓜植物成分在体外和体内均能抑制人t淋巴细胞嗜型病毒（HTLV-1）的感染性。

IF 2.3 4区医学

Journal of NeuroVirology Pub Date : 2025-02-01 Epub Date: 2023-08-02 DOI: 10.1007/s13365-023-01160-0

Sanaz Ahmadi Ghezeldasht, Hamid Reza Bidkhori, Raheleh Miri, Arezoo Baghban, Arman Mosavat, Seyed Abdolrahim Rezaee

{"title":"Momordica charantia phytoconstituents can inhibit human T-lymphotropic virus type-1 (HTLV-1) infectivity in vitro and in vivo.","authors":"Sanaz Ahmadi Ghezeldasht, Hamid Reza Bidkhori, Raheleh Miri, Arezoo Baghban, Arman Mosavat, Seyed Abdolrahim Rezaee","doi":"10.1007/s13365-023-01160-0","DOIUrl":"10.1007/s13365-023-01160-0","url":null,"abstract":"There is an urgent need to find an effective therapy for life-threatening HTLV-1-associated diseases. Bitter melon (Momordica charantia) is considered a traditional herb with antiviral and anticancer properties and was tested in this study on HTLV-1 infectivity. GC-MS analyzed the alcoholic extract. In vitro assay was carried out using transfection of HUVEC cells by HTLV-1-MT2 cell line. The cells were exposed to alcoholic and aqueous extracts at 5,10, and 20 µg/mL concentrations. In vivo, mice were divided into four groups. Three groups were treated with HTLV-1-MT-2 cells as test groups and positive control, and PBS as the negative control group in the presence and absence of M. charantia extracts. Peripheral blood mononuclear cells (PBMCs), mesenteric lymph nodes (MLNs), and splenocytes were collected for HTLV-1-proviral load (PVL) assessment, TaqMan-qPCR. The GC-MS analysis revealed 36 components in M. charantia. The studies showed significant reductions in HTLV-1-PVL in the presence of extract in the HUVEC-treated groups (P = 0.001). Furthermore, the inhibitory effects of extracts on HTLV-1 infected mice showed significant differences in HTLV-1-PVL among M. charantia treated groups with untreated (P = 0.001). The T-cells in MLNs were significantly more susceptible to HTLV-1 than others (P = 0.001). There were significant differences among HTLV-1-infected cells in MLNs and splenocytes (P = 0.001 and 0.046, respectively). Also, aqueous and alcoholic extract-treated groups significantly affected HTLV-1-infected PBMCs (P = 0.002 and 0.009, respectively). M. charantia may have effective antiviral properties. The substantial compound of M. charantia could have inhibitory effects on the proliferation and transmission of HTLV-1 oncovirus.","PeriodicalId":16665,"journal":{"name":"Journal of NeuroVirology","volume":" ","pages":"24-34"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9917734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High glucose potentiates Zika virus induced-astroglial dysfunctions. 高葡萄糖增强寨卡病毒诱导的星形胶质细胞功能障碍。

IF 2.3 4区医学

Journal of NeuroVirology Pub Date : 2025-02-01 Epub Date: 2024-12-03 DOI: 10.1007/s13365-024-01238-3

Natalie Katherine Thomaz, Larissa Daniele Bobermin, Patrícia Sesterheim, Ana Paula Muterle Varela, Thais Fumaco, Marina Seady, Belisa Parmeggiani, Marina Concli Leite, Guilhian Leipnitz, Lucélia Santi, Walter O Beys-da-Silva, Jorge Almeida Guimarães, Paulo M Roehe, Carlos-Alberto Gonçalves, Diogo Onofre Souza, André Quincozes-Santos

{"title":"High glucose potentiates Zika virus induced-astroglial dysfunctions.","authors":"Natalie Katherine Thomaz, Larissa Daniele Bobermin, Patrícia Sesterheim, Ana Paula Muterle Varela, Thais Fumaco, Marina Seady, Belisa Parmeggiani, Marina Concli Leite, Guilhian Leipnitz, Lucélia Santi, Walter O Beys-da-Silva, Jorge Almeida Guimarães, Paulo M Roehe, Carlos-Alberto Gonçalves, Diogo Onofre Souza, André Quincozes-Santos","doi":"10.1007/s13365-024-01238-3","DOIUrl":"10.1007/s13365-024-01238-3","url":null,"abstract":"Zika virus (ZIKV) is a neurotropic flavivirus that induces congenital Zika syndrome and neurodevelopmental disorders. Given that ZIKV can infect and replicate in neural cells, neurological complications in adult brain are also observed. Glial cells may emerge to delay and/or prevent the development of ZIKV-induced neurodegeneration. These cells actively participate in metabolic, inflammatory and redox processes, and consequently, in the pathophysiology of neurodegenerative diseases, including diabetic encephalopathy. In this sense, changes in glucose metabolism can support the inflammatory activity of astroglial cells; however, the effects of increased glucose concentration during ZIKV infection have not yet been explored in astroglial cells. Here, we evaluated functional parameters of astroglial cells exposed to ZIKV upon normal and high glucose concentrations, focusing on inflammatory profile, oxidative stress, and expression of critical genes for astroglial functions. High glucose potentiated the pro-inflammatory and oxidative effects of ZIKV, as well as potentiated the downregulation of signaling pathways, such as Nrf-2 (nuclear factor erythroid derived 2 like 2), sirtuin 1 (SIRT1), peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α), and poly (ADP-ribose) polymerase (PARP). In summary, our results suggest that high glucose can favor the activation of inflammatory signaling while impairing cytoprotective pathways in astroglial cells exposed to ZIKV and reinforce the hypothesis that this virus is highly neurotrophic, with significant impact in glial cells.","PeriodicalId":16665,"journal":{"name":"Journal of NeuroVirology","volume":" ","pages":"84-94"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generating fair, reliable, and accurate neuropsychological test norms for people with HIV in a low- or middle-income country. 为低收入或中等收入国家的艾滋病毒感染者制定公平、可靠和准确的神经心理学测试规范。

IF 2.3 4区医学

Journal of NeuroVirology Pub Date : 2025-02-01 Epub Date: 2024-12-30 DOI: 10.1007/s13365-024-01235-6

H Gouse, K G F Thomas, C J Masson, M Henry, J A Joska, L A Cysique, S Ling, X Ye, J Liu, R N Robbins

{"title":"Generating fair, reliable, and accurate neuropsychological test norms for people with HIV in a low- or middle-income country.","authors":"H Gouse, K G F Thomas, C J Masson, M Henry, J A Joska, L A Cysique, S Ling, X Ye, J Liu, R N Robbins","doi":"10.1007/s13365-024-01235-6","DOIUrl":"10.1007/s13365-024-01235-6","url":null,"abstract":"Effective neuropsychological assessment of people with HIV (PWH) in low- and middle-income countries (LMICs) is hampered by the unavailability of adequate test norms. We aimed to: (1) develop demographically-corrected (regression-based) South African (SA) normative data for an HIV appropriate neuropsychological test battery for Xhosa home-language speakers; (2) compare the utility of those norms to that of (i) internal standardization norms and (ii) US test publisher norms; and (3) determine the criterion validity of the newly-developed norms. 114 controls and 102 demographically comparable Xhosa home-language people living with HIV completed a well-establised, standard HIV neuropsychological test battery assessing seven cognitive domains. Using a common performance metric (z-score), we compared control and PWH test performance and examined the extent to which the three different normative datasets embedded demographic effects e.g., education. Using internal standardization norms, analyses detected medium-sized correlations of overall test performance with age and education. Correlations were fully corrected for by the newly-developed demographically-corrected norms. Using demographically-corrected norms, PWH performed significantly more poorly than controls in five cognitive domains, whereas using internal standardization norms and test-publisher norms, PWH performed significantly more poorly than controls in one and two domains, respectively. Demographically-corrected norms estimated 43.1% of PWH were cognitively impaired; these estimates were 22.5% using test-publisher norms and 19.6% using internal standardization norms. Demographically-corrected SA norms were more sensitive to cognitive impairment in PWH than the other sets of norms. Expansion of this regression-based method to create population-appropriate norms will benefit research and clinical practice in LMICs.","PeriodicalId":16665,"journal":{"name":"Journal of NeuroVirology","volume":" ","pages":"41-55"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The incidence of symptomatic CSF viral escape in patients on antiretroviral therapy in western India: a retrospective cohort study. 印度西部接受抗逆转录病毒治疗的患者中无症状 CSF 病毒逃逸的发生率：一项回顾性队列研究。

IF 2.3 4区医学

Journal of NeuroVirology Pub Date : 2025-02-01 Epub Date: 2024-11-13 DOI: 10.1007/s13365-024-01236-5

Atul Patel, Ketan Patel, Niyati Patel, Kinjal Shah, Ambuj Kumar

{"title":"The incidence of symptomatic CSF viral escape in patients on antiretroviral therapy in western India: a retrospective cohort study.","authors":"Atul Patel, Ketan Patel, Niyati Patel, Kinjal Shah, Ambuj Kumar","doi":"10.1007/s13365-024-01236-5","DOIUrl":"10.1007/s13365-024-01236-5","url":null,"abstract":"Antiretroviral treatment (ART) effectively suppresses viral loads in both plasma and cerebrospinal fluid (CSF). Patients with discordant plasma and CSF viral loads may experience chronic-progressive or fluctuating neurocognitive dysfunctions. This study examined the incidence of symptomatic CSF viral escape (CSFVE) in patients receiving ART. This retrospective cohort study was conducted between 2000 and 2023. The primary outcome measure was the incidence of symptomatic CSFVE. Nonparametric Mann-Whitney U and Fisher exact/χ 2 tests were applied for continuous and categorical variables, respectively. The cumulative incidence function with Gray's test was used to compare the incidence of CSFVE across the treatment regimens. During the study period, 52 of the 8415 patients were diagnosed with CSFVE. The median duration of HIV diagnosis in patients with CSF VE was 150 (12-288) months, with a median nadir CD4 + T-cell count 96.5 (13-601 cells/L)], and 75% of the patients were on a ritonavir-boosted protease inhibitor (PI/r) regimen. The cumulative incidence of symptomatic CSFVE at a follow-up of 14 years was 1% (95% CI, 0-1%). PI/r (HR 34.73; 95% CI 13.5 to 89.4; p < 0.001) and integrase strand transfer inhibitor (INSTI) (HR 3.42; 95% CI 1.94 to 6.02; p < 0.001) regimens were significantly more likely to be associated with CSFVE than the Non-nucleoside reverse transcriptase inhibitors (NNRTIs) regimens. NNRTIs had the lowest risk of CSFVE compared to the PI/r and INSTI regimens. A rapid and complete recovery is possible with symptomatic CSFVE if it is diagnosed and treated early.","PeriodicalId":16665,"journal":{"name":"Journal of NeuroVirology","volume":" ","pages":"75-83"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune response to COVID-19 vaccines among people living with human T-cell lymphotropic virus type 1 infection: a retrospective cohort study from Iran. 人类T细胞嗜淋巴病毒1型感染者对新冠肺炎疫苗的免疫反应：来自伊朗的回顾性队列研究。

IF 2.3 4区医学

Journal of NeuroVirology Pub Date : 2025-02-01 Epub Date: 2023-10-23 DOI: 10.1007/s13365-023-01176-6

Reza Jafarzadeh Esfehani, Zohreh Vahidi, Mohammad Shariati, Arman Mosavat, Azam Shafaei, Maryam Shahi, Houshang Rafatpanah, Hamid Reza Bidkhori, Reza Boostani, Mohammad Reza Hedayati-Moghaddam

{"title":"Immune response to COVID-19 vaccines among people living with human T-cell lymphotropic virus type 1 infection: a retrospective cohort study from Iran.","authors":"Reza Jafarzadeh Esfehani, Zohreh Vahidi, Mohammad Shariati, Arman Mosavat, Azam Shafaei, Maryam Shahi, Houshang Rafatpanah, Hamid Reza Bidkhori, Reza Boostani, Mohammad Reza Hedayati-Moghaddam","doi":"10.1007/s13365-023-01176-6","DOIUrl":"10.1007/s13365-023-01176-6","url":null,"abstract":"The effectiveness of COVID-19 vaccination is still unclear in individuals with underlying diseases such as HTLV-1 infection. This retrospective cohort study aimed to evaluate the humoral response of COVID-19 vaccines among people living with HTLV-1 (PLHTLV) in northeastern Iran. From December 2021 to October 2022, eighty-six HTLV-1+ subjects (50 males and 36 females; 47.7 ± 11.2 years) and 90 HTLV-1 seronegative individuals (age- and sex-matched convenient samples) were enrolled. The humoral immune response was evaluated by measuring different COVID-19 Abs in serum samples at least 28 days after receiving 2nd or 3rd doses of COVID-19 vaccines. Throughout all three rounds of immunization, Sinopharm was the most commonly used COVID-19 vaccine across all three immunization rounds. Compared to the HTLV-1- group, a significantly lower frequency of all four Abs activity was observed among PLHTLV:anti-nucleocapsid (66.3% vs 86.7%, p = 0·001), anti-spike (91.9% vs 98.9%, p = 0·027), RBD (90.7% vs 97.8%, p = 0·043), and neutralizing Abs (75.6% vs 95.5%, p < 0·001). Also, the frequency of all Abs in 28 patients with HAM/TSP was higher than that of 58 asymptomatic carriers, although this difference was statistically significant only in the case of anti-spike Abs (p = 0.002). Notably, PLHTLV-vaccinated against COVID-19 demonstrated significantly lower antibody activities, indicating a reduced humoral immune response to COVID-19 vaccines.","PeriodicalId":16665,"journal":{"name":"Journal of NeuroVirology","volume":" ","pages":"35-40"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49691020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA in neuroexosome as a potential biomarker for HIV-associated neurocognitive disorders. 神经外泌体中的MicroRNA作为hiv相关神经认知障碍的潜在生物标志物。

IF 2.3 4区医学

Journal of NeuroVirology Pub Date : 2025-02-01 Epub Date: 2025-01-16 DOI: 10.1007/s13365-024-01241-8

Kotaro Arizono, Ayako Sedohara, Khulan Tuvshinjargal, Takahiro Tanaka, Michiko Koga, Fumio Nakahara, Amato Ootani, Yoshiaki Kanno, Kazuhiko Ikeuchi, Makoto Saito, Eisuke Adachi, Takeya Tsutsumi, Hiroshi Yotsuyanagi

{"title":"MicroRNA in neuroexosome as a potential biomarker for HIV-associated neurocognitive disorders.","authors":"Kotaro Arizono, Ayako Sedohara, Khulan Tuvshinjargal, Takahiro Tanaka, Michiko Koga, Fumio Nakahara, Amato Ootani, Yoshiaki Kanno, Kazuhiko Ikeuchi, Makoto Saito, Eisuke Adachi, Takeya Tsutsumi, Hiroshi Yotsuyanagi","doi":"10.1007/s13365-024-01241-8","DOIUrl":"10.1007/s13365-024-01241-8","url":null,"abstract":"HIV-associated neurocognitive disorder (HAND) is a complication of chronic inflammation caused by HIV infection that impairs cognitive and motor functions. HAND can occur at any age, regardless of the duration of infection, even in people living with HIV (PLWH) whose blood viral load is controlled by antiretroviral therapy. The diagnosis of HAND requires a battery of neuropsychological tests, which is time-consuming and burdensome, limiting its effectiveness for screening PLWH. Here, we aimed to identify biomarkers for quantitatively diagnosing and screening for HAND using minimally invasive blood tests. Neuronal-derived exosomes (neuroexosomes) were isolated from the peripheral blood of PLWH, and the transcriptomes of their microRNAs (miRNAs) were analyzed. We identified five upregulated miRNAs (hsa-miR-16-5p, hsa-miR-26a-3p, hsa-92a-3p, hsa-miR-103a-3p, and hsa-miR-185-5p), and two downregulated miRNA (hsa-miR-3613-3p and hsa-miR-4668-5p) in PLWH diagnosed with HAND (HAND PLWH). Functional analysis of five miRNAs whose expression levels increased in HAND PLWH using the database showed that these miRNAs are involved in motor proteins and endocytosis, which are associated with nerve function. The expression levels of hsa-miR-16-5p, hsa-miR-103a-3p, and hsa-miR-185-5p were significantly higher than those in the non-HIV controls and non-HAND PLWH, suggesting that these miRNAs are potential biomarkers for HAND. Since there were no changes in known dementia miRNA biomarkers in HAND PLWH, the miRNAs identified in this study will allow for early differentiation of HAND.","PeriodicalId":16665,"journal":{"name":"Journal of NeuroVirology","volume":" ","pages":"56-74"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of plasma exosomes from women living with HIV Stratified by HAND on monocyte subpopulations from healthy women without HIV. 由HAND分层的HIV女性血浆外泌体对健康无HIV女性单核细胞亚群的影响

IF 2.3 4区医学

Journal of NeuroVirology Pub Date : 2025-02-01 Epub Date: 2025-01-30 DOI: 10.1007/s13365-024-01240-9

Bryan Jael Collazo, Lorivette Ortiz-Valentín, Cristhian G Negrón-Rodríguez, Juan Carlos Medina-Colón, Yisel M Cantres-Rosario, Elaine Rodríguez, Valerie Wojna, Yamil Gerena

{"title":"Influence of plasma exosomes from women living with HIV Stratified by HAND on monocyte subpopulations from healthy women without HIV.","authors":"Bryan Jael Collazo, Lorivette Ortiz-Valentín, Cristhian G Negrón-Rodríguez, Juan Carlos Medina-Colón, Yisel M Cantres-Rosario, Elaine Rodríguez, Valerie Wojna, Yamil Gerena","doi":"10.1007/s13365-024-01240-9","DOIUrl":"10.1007/s13365-024-01240-9","url":null,"abstract":"The role of plasma exosomes from people living with HIV (PLWH) with HAND in the phenotypic profile of uninfected monocytes remains unknown. We hypothesized that these exosomes influence the CD14/CD16 phenotypical profile of uninfected monocytes in a time-dependent manner. Exosomes were collected via ultracentrifugation from the plasma of women living with HIV (WLWH) and healthy controls stratified according to their cognition into normal cognition (NC) or symptomatic neurocognitive impairment (SNI) groups. Monocyte subsets were identified via flow cytometry by using anti-CD14 and anti-CD16 fluorescent antibodies. Exosome uptake and changes in the percentages of monocyte subpopulations were analyzed from 1 to 24 h. The following results were obtained. (1) The uptake of HIV-negative exosomes by total uninfected monocytes was observed at 24 h, whereas the uptake of HIV-positive exosomes was observed at an earlier time point at 6 h. (2) HIV-positive exosomes significantly decreased the percentage of classical monocytes and increased intermediate and nonclassical monocytes at 24 h. (3) The uptake of NC exosomes was observed at an early time point at 6 h compared with SNI in all of the monocyte subsets. (4) Higher percentages of monocyte subsets were observed when cells were exposed to NC exosomes at 1 h, 6 h, or 24 h than when monocytes were exposed to exosomes from SNI patients. Our findings may help to identify new targets and molecular mechanisms that are involved in the pathogenesis of HAND.","PeriodicalId":16665,"journal":{"name":"Journal of NeuroVirology","volume":" ","pages":"95-107"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0