Journal of Parasitic Diseases最新文献

{"title":"Occurrence of nematodes, <i>Equinurbia sipunculiformis</i> and <i>Quilonia renniei</i> in a wild male elephant: a case report.","authors":"S T Bino Sundar, Thirunagari Ramanujadas, K Rajesh Kumar, B Nishanth, S Arunkumar, A Sangaran, Bhaskaran Ravi Latha","doi":"10.1007/s12639-024-01733-8","DOIUrl":"10.1007/s12639-024-01733-8","url":null,"abstract":"<p><p>The occurrence of the nematodes, <i>Equinurbia sipunculiformis</i> and <i>Quilonia renniei</i> in the intestinal tract of a six-year-old, free ranging male elephant (<i>Elephas maximus</i>) observed during necropsy is reported. The worms were collected in 10% formalin and processed. <i>E. sipunculiformis</i> worms were stout, males were shorter than females. A total of 65 worms were recovered out of which 45 were male worms and 20 were female worms. They had a characteristic head with a cuticular prominence and a sub globular cup shaped buccal capsule without teeth. The external leaf crown was characteristic projecting above the anterior end of the worm. The oesophagus was enlarged immediately behind the buccal capsule, then cylindrical, and gradually enlarged. Male worms had a well-developed bursa at the posterior end with equal, alate and ornamentated spicules with elongated lateral bursal lobes. Female worms had a tapered tail with a conical prominence and a vulval opening at its summit. Uterus was filled with thin shelled eggs. <i>Q. renniei</i> male worms were shorter in length than female worms. A total of 150 worms were recovered out of which 110 were male worms and 40 were female worms. Cuticular striations were observed. Mouth collar and buccal capsule were present. The external leaf crown projected beyond the surface of the head. Male worms had a well-developed bursa and spicules. The dorsal ray of the bursa was comparatively short. The lateral and ventral rays were slender. Female worms had a pointed tail. The vulva was situated at a distance from the tip of the tail. Uterus was filled with oval thin shelled eggs. Pathological findings included pale mucous membrane, anaemia and haemorrhagic enteritis. The present observations on the occurrence of nematode infection with <i>E. sipunculiformis</i> and <i>Q. renniei</i> will be important to map the status of nematodiasis in elephants in wild and to frame strategies for control of intestinal nematodiasis in wild elephants.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 1","pages":"62-68"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evasive mechanisms of human VSG and PfEMP1 antigens with link to Vaccine scenario: a review.","authors":"Okechukwu Anthony Obi, Rose Nduka Obiezue, Desmond Eze, David Adeyinka Adebote","doi":"10.1007/s12639-024-01740-9","DOIUrl":"10.1007/s12639-024-01740-9","url":null,"abstract":"<p><p>Recent fights on the control of trypanosomiasis and malaria focused on underscoring the concepts of antigen evasive mechanisms with the view to exploit the defensive mechanisms inherent in VSG and PfEMP1, although giant strides is being achieved towards beating the antigenic propensity of malaria parasites. <i>Trypanosoma</i> and <i>Plasmodium falciparum</i> adopt a common antigenic novelty through alternate expression of VSG and PfEMP1 respectively. These immunodominant antigens sterically shield other surface proteins from host antibodies and unvaryingly turn out to be the requisite elements with difficult underlining immunological concept for unmatched escape mechanisms of vaccine actions. Hence, the uncommon role of the pathogens to brazenly circumnavigate immunity through switching of variant antigens has not kept pace. Switching of variant surface in human trypanosomes occurs through programmed DNA rearrangements while in <i>P</i>. <i>falciparum</i>, switching occurs by purely transcriptional mechanism. The repertoire genes harmonize evasion of human immunity and also rekindle the outcome of infections. The extensive sequence divergence and genetic polymorphism of VSG and PfEMP1 are the requisite elements for the next generation breakthrough in vaccine discoveries. Thus, the springboard for the development of novel targets is lurking with the wit of unraveling the immunological concepts underlining the evasive aptitude of VSG and PfEMP1 with convincing biochemical techniques, hence offering a blueprint for enhanced vaccine targets. This review elucidates evasive mechanisms of VSG and PfEMP1 with link to pathologies, challenges of antigenic switches and prospects to current vaccine scenario.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 1","pages":"13-28"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11833005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic profile of <i>Echinococcus granulosus</i> isolated from the livestock and human in northwest Iran.","authors":"Saeed Arabloo, Morteza Ghanbari Johkool, Mohammad Ali Mohammadi, Alireza Mohammadzadeh, Davoud Mohammadi, Majid Fasihi Harandi, Mehrzad Saraei, Peyman Heydarian, Milad Badri, Elham Hajialilo","doi":"10.1007/s12639-024-01738-3","DOIUrl":"10.1007/s12639-024-01738-3","url":null,"abstract":"<p><p>Cystic echinococcosis (CE) is categorized among the highly important zoonoses, occurring in many regions of the world. Iran is a significant endemic focus of CE, where it poses substantial economic and public health challenges to local communities. The current study aimed to identify the genotype and species profile of <i>E. granulosus</i> among the patients and livestock in Qazvin, Iran. A total of 80 hydatid cyst samples were collected from humans as well as farm animals including sheep, cattle, and goats. DNA extraction and PCR-sequencing were conducted targeting the mitochondrial CO1 and ND1 regions. Molecular phylogeny and haplotype network analysis was calculated for the isolates. Out of 79 evaluated samples, 97.5% belonged to G1 genotype and 2.5% to G3 genotype. The G1 genotype was present in all infected participants. Also, the study showed the G1 to be the most common genotype of <i>E. granulosus</i> among both the humans and the animals examined. Furthermore, our results emphasize on the importance of the sheep-dog cycle in public health.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 1","pages":"142-148"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antiparasitic effect of C-Phycocyanin nanoparticles on cryptosporidiosis in immunosuppressed mice.","authors":"Esraa Abdelhamid Moneer, Sara H Akl, Yahya H Shahin, Sendianah H Shahin, Bassma H Elwakil, Areej Eskandrani, Keshav Raj Paudel, Basant A Bakr","doi":"10.1007/s12639-024-01739-2","DOIUrl":"10.1007/s12639-024-01739-2","url":null,"abstract":"<p><p>The present study aimed to newly synthesize C-Phycocyanin nanoparticles through a ball-milling technique. C-Phycocyanin nanoparticles had average diameter of 290.2 nm, zeta potential 48.3 mV and 0.390 PDI. The synthesized nanoparticles were tested as an anti-cryptosporidiosis agent compared to the crude C-Phycocyanin. Cryptosporidiosis induction was done in immunocompetent and immunosuppressed mice. The antiparasitic effect was evaluated through multiplex qualitative PCR, count of oocysts, serum biochemical parameters, oxidative stress and antioxidant markers, cytokine analysis, and histopathological study (qualitative and quantitative). Multiplex qualitative PCR analysis revealed the presence of the tested parasite gene (JVAF) in all the treated groups. The percentage of the highest reduction of the oocysts means counting has been detected in the infected mice treated with nitazoxanide (NTZ) (Ic, IIc) followed by treatment with C-Phycocyanin nano (Ie, IIe), then C-Phycocyanin (Id, IId) (42, 48, 37, 36 15, and 29% respectively). C-Phycocyanin and C-Phycocyanin nanoparticles treated groups dramatically affected the levels of catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA) activity. Moreover, treatment with C-Phycocyanin and C-Phycocyanin nanoparticles significantly reduced cytokines levels (Tumor necrosis factor-alpha (TNF-α), interferon-gamma (INF-γ), and interleukin (IL-13)) in contrast to untreated groups. The histological results in the tissues of mice's ileum which are infected by <i>Cryptosporidium</i> spp. (positive control) exhibited cellular inflammation in the submucosa and lamina properia, as well as thickening and flattening of the villi. However, the application of nanoparticles allowed the villus to grow further, indicating the nano impact of the cryptosporidiosis treatment.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at (10.1007/s12639-024-01739-2).</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 1","pages":"173-185"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11833032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological diagnosis of <i>Toxocara canis</i> in mothers and their neonate referred to selected teaching hospitals, Tehran, Iran, 2020.","authors":"Malihe Mohebi, Somayeh Aghamolaei, Mohammad Reza Sohrabi, Hooshang Khazan, Ali Rostami","doi":"10.1007/s12639-024-01725-8","DOIUrl":"10.1007/s12639-024-01725-8","url":null,"abstract":"<p><p>Toxocariasis is a common zoonotic parasitic disease, primarily caused by the larvae of <i>Toxocara canis</i> or <i>T. cati</i>. Humans become infected through accidental ingestion of infective eggs in soil contaminated with feces of infected animals, or by consuming undercooked meat from paratenic hosts harboring <i>Toxocara</i> larvae. This cross-sectional study aimed to evaluate the seroprevalence of <i>Toxocara</i> spp. in 250 mothers and their 250 neonates referred to selected teaching hospitals affiliated with Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2020. Data were recorded using a specialized questionnaire, and serum and peripheral blood smears (for eosinophil count) were collected. Serum samples were tested using the ELISA serological test for IgG antibodies against <i>Toxocara</i> spp. The collected data were analyzed using IBM SPSS Statistics software version 21.0. A chi-square test was performed, and a <i>P</i> value less than 0.05 was considered statistically significant. Among the 500 participants, 24 cases (4.8%) tested positive for anti-<i>Toxocara canis</i> IgG antibodies. Seropositive cases included 6 mothers only (25%), 6 neonates only (25%), and 12 cases both mother and their neonate (50%). Statistical analysis showed a significant relationship between the presence of anti-<i>Toxocara</i> antibodies and hospital type and nationality (<i>P</i> value < 0.05). However, no significant relationship was found between the presence of antibodies and other demographic variables such as age, sex, eosinophilia, and history of contact with animals in mothers and their neonates (<i>P</i> value > 0.05). This study indicates a substantial rate <i>Toxocara</i> infection in mothers and neonates, necessitating appropriate preventive measures by the relevant authorities.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"944-949"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of fasciolosis in Nepal: epidemiology, diagnosis, and control strategies.","authors":"Sameer Thakur","doi":"10.1007/s12639-024-01700-3","DOIUrl":"10.1007/s12639-024-01700-3","url":null,"abstract":"<p><p>Fasciolosis, caused by <i>Fasciola hepatica</i> and <i>Fasciola gigantica</i>, is a globally prevalent vector-borne disease that affects livestock and humans, with significant economic and zoonotic implications. In Nepal where livestock contributes to 11.5% of the GDP, fasciolosis poses a significant threat to the agriculture sector. The country's diverse geographical and climatic conditions contribute to the widespread prevalence of the disease. Domestic ruminants, including buffalo, cattle, goats, and sheep are extensively affected, with a prevalence rate ranging from 10.25% in goats to 68% in buffalo. The disease also impacts high-altitude regions, affecting even yaks in the Himalayas. Human infections are also on the rise, highlighting the zoonotic nature of the disease. This review provides a comprehensive and in-depth analysis of fasciolosis in Nepal, emphasizing its pathogenesis, epidemiology, and diagnosis. The review also assesses the strategies for controlling fasciolosis in Nepal, including anthelmintic treatments, vector control, and farm management practices. The challenges and limitations of these methods are discussed, underscoring the need for alternative strategies to prevent drug resistance and enhance disease management. Additionally, this paper proposes future directions for the management of fasciolosis in Nepal to provide a comprehensive and effective approach for controlling and managing the disease in the country.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"723-735"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A checklist of the monogenean parasites (Platyhelminthes) of fishes of northeast India.","authors":"Leki Wangchu, Dobiam Narba, Chawan Matey","doi":"10.1007/s12639-024-01717-8","DOIUrl":"10.1007/s12639-024-01717-8","url":null,"abstract":"<p><p>A checklist was generated by compiling a database of all available published accounts of the monogenean parasites of fish from northeast India. The checklist is presented as a host-parasite list, including 35 nominal species from 14 genera and 3 families of monogeneans. Ten of the monogenean species listed here have been described from native fishes in the region, while 25 had previously been described from other parts of India. Dactylogyridae was the dominant family with 33 species, followed by one species each from the family Sundanonchidae and Diplozoidae. <i>Wallago attu</i> was the most examined fish and was represented by five parasite species. This study is an important step for managing and controlling parasite diseases in regional aquaculture and also for future research on the ecology, evolutionary biology, and biogeography of monogenean parasites and their hosts. More significant efforts are needed to fully understand the diversity of monogenean parasites from the rich fish fauna of northeast India.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"762-766"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimalarial efficacy of <i>Duttaphrynus melanostictus</i> skin extract via inhibition of <i>Plasmodium falciparum</i> Na⁺/H⁺ ATPase.","authors":"Akshay D Bagwe, Roshan C D'Souza, Bharatbhushan B Sharma","doi":"10.1007/s12639-024-01716-9","DOIUrl":"10.1007/s12639-024-01716-9","url":null,"abstract":"<p><p>Malaria remains a major health issue worldwide that affects many people, particularly in developing nations. Since, the malarial parasite has developed resistance against nearly every antimalarial drug now in use, it is imperative to search for novel antimalarial medications. Toxins produced by skin glands of toads have been shown to possess antiparasitic properties against a variety of protozoan parasites because of the bufadienolides they contain. Even though several studies have been conducted to show that toad skin secretions have antimalarial properties, very little information is known about the precise mechanism by which they work against <i>Plasmodium</i> infection. Thus, the goal of this study was to evaluate the antiplasmodial activity of crude skin extracts from Common Asian Toads, <i>Duttaphrynus melanostictus</i>, of different sizes and illustrate how they work against <i>Plasmodium falciparum</i> 3D7 cells. The findings demonstrated a negative correlation between the toad size and percent yield of the extracts. HPTLC and UPLC-MS/MS analysis of the extracts exhibited varied composition of bufadienolides depending on the size of the animal. The extract obtained from small toads containing resibufagin and marinobufagin lactate demonstrated highest antiplasmodial activity and showed lowest cytotoxicity on peripheral blood mononuclear cells. It was discovered that the extract was effective against the trophozoite stage of the parasite. The extract was reported to inhibit Na⁺/H⁺ ATPase of <i>Plasmodium</i> by binding to sodium-enzyme complex at ATP binding site. The study offers baseline data that can be used to assess the antimalarial potential of individual components in the skin extract derived from small toads.</p><p><strong>Graphical abstract: </strong></p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12639-024-01716-9.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"831-848"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cross-sectional study on the prevalence and associated risk factors for intestinal parasitic infections among under-five aged children in Dabat primary hospital, north Gondar, Ethiopia.","authors":"Yamlaksira Amare, Tilahun Yohannes, Solomon Tesfaye","doi":"10.1007/s12639-024-01708-9","DOIUrl":"10.1007/s12639-024-01708-9","url":null,"abstract":"<p><p>Intestinal parasitic infections (IPIs) are the main public health problems in several developing countries, and under-five-aged children are the most vulnerable groups of the population. The current study aimed to determine the prevalence and associated risk factors of IPIs among under-five-aged children at Dabat primary hospital, northwest Ethiopia. A cross-sectional hospital-based study including 384 under-five-aged children was conducted from February to June 2022. A structured questionnaire was used to collect relevant information on risk factors, and stool samples were collected and examined using wet mount and sedimentation techniques to determine prevalence. Chi-square and logistic regression were used to evaluate the possible association and the strength of the association between dependent and independent variables. The overall prevalence of intestinal parasites was 32.81%. Ten species of intestinal parasites were identified. <i>Entamoeba histolytica / dispar</i> was the predominant intestinal parasite species, with a prevalence of 7.55%. <i>Giardia lamblia</i> and hookworm had prevalence of 6.77% and 5.47% respectively. Those children whose Mother/guardian do not wash their hand before feeding their child are more than five time infected than those who do wash (AOR = 5.26, CI = 2.28-12.2, <i>p</i> < 0.001). Children who do not wear shoe are more infected than who did (AOR = 14.5, CI = 5.77-36.5, <i>p</i> < 0.001). Several risk factors were identified in this study among these washing hands after toilet before touching child, washing hands before feeding their child, Child meal and habit of shoes wearing were the main predictors of IPI. Since the prevalence of IPI observed in this study is that of under-five-aged children; raising awareness of mothers/guardians about how to keep hygiene; transmission ways and health impacts of IPIs; and proper way of feeding their children to avoid risk is crucial.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"789-801"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of seasonal variability on the increased malaria positivity rate in drought-prone malaria endemic areas of Ethiopia.","authors":"Selomon Tefera, Temesgen Bekele, Tsige Ketema","doi":"10.1007/s12639-024-01720-z","DOIUrl":"10.1007/s12639-024-01720-z","url":null,"abstract":"<p><p>Malaria is a climate-sensitive and season-dependent disease that mainly affects the populations of tropical and subtropical regions of the world. Climatic variables such as rainfall, temperature, and humidity are identified as the driving factors affecting the epidemiology of malaria. In Ethiopia, recently unexpectedly high malaria burden accompanied by substantial morbidity and mortality is observed. The incidence rate of vector-borne diseases such as malaria is largely influenced by fluctuation of seasonal variables due to the proceeding global warming. Therefore, the study aimed to assess the impact of seasonal and climate variability on the burden of malaria in one of drought-prone areas of north central Ethiopia. The study was conducted in the Oromia zone of the Amhara regional state in Ethiopia. Data from patients tested and diagnosed with malaria between 2014 and 2019 were extracted from the medical records of the patients registered in all health facilities found in the zone. Additionally, meteorological data such as rainfall and temperature (maximum and minimum) were collected from the regional meteorological agency. Data were analyzed using R software. The malaria positivity rate recorded in this study was 12.7% (64,522/508,057). Months from June to December were significantly (<i>p</i> < 0.05) associated with high malaria positivity. Rainfall had a significant negative effect on malaria positivity (<i>p</i> = 0.042). Also, three to four months of lagged rainfall is associated with high malaria positivity (<i>p</i> < 0.05). However, monthly minimum temperature positivity influenced the rate of malaria positivity (exp = 0.037, <i>p</i> = 0.0098). While monthly mean maximum temperature showed a negative association with malaria positivity, although it was insignificant. In addition, malaria positivity significantly decreased from 2014 to 2019 by 20.5% (exp = 0.205, <i>p</i> < 0.0001). The seasonal and climatic variables evaluated in this study substantially affected the malaria positivity rate. Monthly mean temperature was the leading and predictor variable for malaria infection. Despite vigorous efforts by health authorities in the country at this level, following the neglected implementation of interventional activities in the nation for a couple of years, malaria-associated burdens have increased. As seasonal and climate variables could play a key role in the rising burden, concerned national or local health authorities, and policymakers should prioritize urgent interventional activities to control malaria in one of the drought-prone areas of Ethiopia.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"860-871"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}