Yasaman Karimi, Abazar Roustazadeh, Hamed Mir, Kavous Solhjoo, Manoochehr Shabani, Enayatollah Shadmand, Ali Taghipour, Amir Abdoli, Bahareh Bahadori Mazidi
{"title":"Seroprevalence of <i>Toxocara</i> spp. infection among pregnant women in Jahrom city in Fars province, southern Iran.","authors":"Yasaman Karimi, Abazar Roustazadeh, Hamed Mir, Kavous Solhjoo, Manoochehr Shabani, Enayatollah Shadmand, Ali Taghipour, Amir Abdoli, Bahareh Bahadori Mazidi","doi":"10.1007/s12639-024-01755-2","DOIUrl":"10.1007/s12639-024-01755-2","url":null,"abstract":"<p><p><i>Toxocara</i> spp. infection can cause systemic and ocular disease in infants and pregnant women. In this study, the seroprevalence and associated risk factors of <i>Toxocara</i> spp. infection were investigated in pregnant women from Jahrom county, Fars province, southern Iran. A total of 220 serum samples from pregnant women were assessed for the presence of <i>Toxocara</i> spp. IgG by enzyme-linked immunosorbent assay (ELISA). The associated risk factors were obtained using questionnaires. The seroprevalence was 16.36% (36/220) among pregnant women. As such, a statistically significant association was found between age (39 ≤ years old with <i>P-</i>value: 0.009), consuming raw vegetables (<i>P-</i>value: 0.001), and eating raw or undercooked meat (<i>P-</i>value: 0.04). We recommend an appropriate health education program for pregnant women and serological screening for toxocariasis before pregnancy. Moreover, we believed a need for more epidemiological studies for better understand the status of <i>Toxocara</i> spp. in pregnant women.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 2","pages":"281-286"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seif Ali Mahdavi, Saeed Kargar Sulaimanabad, Farhang Babamahmoodi, Maysam Rezapour
{"title":"The patterns of diagnosis approaches, involved organs, and clinical symptoms and signs of hydatid cyst patients: a case series study from Northern Iran.","authors":"Seif Ali Mahdavi, Saeed Kargar Sulaimanabad, Farhang Babamahmoodi, Maysam Rezapour","doi":"10.1007/s12639-024-01770-3","DOIUrl":"10.1007/s12639-024-01770-3","url":null,"abstract":"<p><p>Hydatid cyst is a disease caused by <i>Echinococcus granulosus</i> parasite and a common zoonotic infection between humans and animals, with increasing prevalence in Iran. Few studies have been conducted in Iran about its clinical epidemiology. This study by Latent class analysis (LCA), examined the patterns of patients based on clinical symptoms and signs, organs involved, and diagnosis approaches and the chi-square test was used to examine the relationship of these patterns with some epidemiologic variables. In this case series study of surveillance system of hydatid cyst of Mazandaran province in north Iran, all patients who were confirmed to have hydatid cysts between 2012 and 2022 (118 patients) were evaluated Liver, lung and belly were the most involved organs and belly pain, liver enlargement and chest pain were also the most common symptoms of the disease. Based on clinical signs and symptoms, patients were classified into two classes: \" non-specific symptoms& sings with 49.2%\" and \" specific symptoms & sings with 50.8%\". Based on the involved organs, the patients were classified into 3 classes: \"specific lung with 17.8%\", \"non-specific organs with 13.6%\" and \"specific liver with 68.6%\". Based on diagnosis approaches, patients were classified into 3 classes, including \"specifically CT-scan with 32.2%\", \" specifically MRI with 33.9%\" and \"specifically X-ray with 33.9%\". More \"specific symptoms & sings pattern\" were seen in the \"specifically liver class\" (almost 60% of cases) and \"non-specific organs class\" (75% of cases). This study provides valuable insights into the clinical epidemiology of hydatid cyst.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 2","pages":"479-488"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A comprehensive review of cerebral malaria.","authors":"Pooja Beniwal, Jyoti Joshi, Sukhbir Kaur","doi":"10.1007/s12639-024-01758-z","DOIUrl":"10.1007/s12639-024-01758-z","url":null,"abstract":"<p><p><i>Plasmodium falciparum</i> malaria presents a substantial threat as an infectious disease causing the most severe neurological complication- Cerebral malaria (CM). CM is responsible for approximately 400,000 annual fatalities among African children, constituting over 90% of the total deaths attributed to malaria. Remarkably, the mortality rate for children succumbing to severe malaria complicated by CM has remained relatively stable over the past decade, ranging between 0.15 and 0.25. The pathogenesis of CM is poorly understood, innumerable theories have explained the pathophysiological mechanisms but hitherto there are no firm conclusions about it. Insufficient access to human samples poses a significant obstacle to advancing research in Cerebral Malaria (CM). However, various post-mortem histopathological studies of human tissues and mice models of CM have revealed the parasite's adherence within the cerebral microvasculature. This adherence is thought to be crucial in the development of the condition, contributing to subsequent pathological alterations in the surrounding tissue and ultimately leading to neural dysfunction. It also leads to the breakdown of the Blood-Brain Barrier leading to haemorrhages. This clinical syndrome is primarily characterized by a significant impairment in consciousness or a state of coma. Patients who survive often experience prolonged neurological complications, such as hemiplegia, partial paralysis, seizure disorders, ataxia, changes in behaviour, and cognitive deficits. To date, several adjunctive treatment approaches have yielded limited success, and numerous attempts to develop effective neuroprotective adjunctive therapies have failed, emphasizing the pressing need for advancements in this area.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 2","pages":"257-272"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Altaf Ahmad Reshi, Hidayatullah Tak, Kamal Hashan Bulbul, Zahoor Ahmad Wani, Abid Hussain Bhat
{"title":"Challenges and insights in morphological identification of <i>Eimeria</i> species in cattle: a comprehensive study and identification key.","authors":"Altaf Ahmad Reshi, Hidayatullah Tak, Kamal Hashan Bulbul, Zahoor Ahmad Wani, Abid Hussain Bhat","doi":"10.1007/s12639-024-01759-y","DOIUrl":"10.1007/s12639-024-01759-y","url":null,"abstract":"<p><p>This study aimed to enhance morphological identification of <i>Eimeria</i> species in cattle of Kashmir through comprehensive analysis of oocyst morphology, body shape index, micropyle presence, dimensions, and sporulation time and prepare a taxonomic key for the identification of <i>Eimeria</i> in cattle. A total of 1198 faecal samples were collected by per rectal method with 589 (49.2%) testing positive for <i>Eimeria</i> species. Subsequently, 845 <i>Eimeria</i> oocysts from various samples were analyzed for shape assessment and measurement of dimensions using micrometry. Nine <i>Eimeria</i> species were identified based on morphological characteristics, including <i>E. zuernii</i>, <i>E. bovis</i>, <i>E. alabamensis</i>, <i>E. auburnensis</i>, <i>E. canadensis</i>, <i>E. subspherica</i>, <i>E. ellipsoidalis</i>, <i>E. cylindrica</i>, and <i>E. brasiliensis.</i> Additionally, an unidentified <i>Eimeria</i> species exhibiting morphological similarities to <i>E. alabamensis</i> and <i>E. bovis</i> was discovered, distinguished by a distinct and conspicuous micropyle absent in either species. Emphasis was placed on identifying recurring characteristics within certain oocysts, and a key was formulated to enhance oocyst identification. Our findings contribute to the understanding of <i>Eimeria</i> species diversity in cattle populations, underscoring the importance of comprehensive morphometric analysis for accurate species identification and epidemiological surveillance. Further research is warranted to elucidate the genetic diversity and pathogenicity of identified species, as well as to refine identification methods and develop effective disease management strategies.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 2","pages":"359-367"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mauricio Laterça Martins, Ana Paula de Souza, João Gabriel Miranda Lessa, Elenice Martins Brasil, Caio Magnotti, Mônica Yumi Tsuzuki, Scheila Anelise Pereira Dutra
{"title":"The cleaner fish <i>Elacatinus figaro</i> can control the monogenean <i>Neobenedenia melleni</i>, an ectoparasite of Lebranche mullet <i>Mugil liza</i> and does not decrease the hematological response.","authors":"Mauricio Laterça Martins, Ana Paula de Souza, João Gabriel Miranda Lessa, Elenice Martins Brasil, Caio Magnotti, Mônica Yumi Tsuzuki, Scheila Anelise Pereira Dutra","doi":"10.1007/s12639-024-01775-y","DOIUrl":"10.1007/s12639-024-01775-y","url":null,"abstract":"<p><p>This study aimed to evaluate the hematological parameters of mullet (<i>Mugil liza</i>) naturally parasitized by <i>Neobenedenia melleni</i> after cohabitation with the barber neon goby (<i>Elacatinus figaro</i>) as a biological control (cleaner fish) of ectoparasites. A total of 108 mullets were distributed in 500 L tanks, with six mullets per tank, in triplicate, divided into six groups: naïve (without neon and non-parasitized), mullets parasitized with <i>N. melleni</i> in cohabitation with one, two, three, and six neons, and the control (without neon and parasitized). Continuous aeration in a static system and a 12-h photoperiod for a period of 25 d was used. For hematological analyses, blood samples were collected from six fish per experimental unit. Blood was used to determine the hematocrit, hemoglobin concentration, total erythrocyte count, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), total thrombocyte and white blood cell counts, as well as the differential leukocyte count. Erythrocyte and lymphocyte counts were higher in the only parasitized group without cleaner fish (control) compared to the groups that had 1, 2, 3 and 6 neon and parasitized with <i>N. melleni</i> and the naïve group. In addition, the mullet circulating monocytes increased in the control groups and in the animals that were cohabiting with 6 neons compared to the naïve. The MCHC was reduced in the control group compared to naïve. Fish from treatments with 1, 2, and 3 neons showed similar hemogram values among themselves and to the naïve group, indicating lower stress in aquaculture systems with <i>E. figaro</i> acting as a biological control.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 2","pages":"472-478"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Efficacy of medicinal plants in cryptosporidiosis treatment: a comprehensive review.","authors":"Shabnam Thakur, Richa Yadav, Upninder Kaur, J S Thakur, Rakesh Sehgal","doi":"10.1007/s12639-024-01769-w","DOIUrl":"10.1007/s12639-024-01769-w","url":null,"abstract":"<p><p><i>Cryptosporidium</i> spp. infect the microvillus epithelial cells of the gastrointestinal tract of vertebrates. It leads to diarrheal disease named as cryptosporidiosis which is considered as the fifth leading cause of diarrhea in children younger than 5 years. The treatment is also limited, as there is only single FDA approved drug (i.e., Nitazoxanide) which has varying efficacy in immune-compromised individuals (HIV infections) and children. The increasing utilization of traditional plants extracts in treating these spp. of infectious protozoan parasite indicated their potentials as alternative remedies for dealing with these detrimental pathogens.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 2","pages":"273-280"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amira Mamdouh Mohamed, Amany Mohammed Hegab, Samar Ibrahim Ismail Amer, Rabab Sayed Zalat, Eman Yassien Shoeib, Iman Raafat Abdel-Shafi
{"title":"Evaluation of the prophylactic and therapeutic effects of selenium and vitamin E on the muscular phase of experimental <i>Trichinella spiralis</i> infection in mice.","authors":"Amira Mamdouh Mohamed, Amany Mohammed Hegab, Samar Ibrahim Ismail Amer, Rabab Sayed Zalat, Eman Yassien Shoeib, Iman Raafat Abdel-Shafi","doi":"10.1007/s12639-024-01762-3","DOIUrl":"10.1007/s12639-024-01762-3","url":null,"abstract":"<p><p>Trichinellosis is a foodborne parasitic infection, where the <i>Trichinella spiralis</i> larvae invade, grow and dwell in muscle cells, transforming them into nurse cells for nutrient supply and protective shelter. The present study evaluated the prophylactic and therapeutic impact of the antioxidants selenium (Se) and vitamin E (Vit E), alone and in combinations with albendazole (ALB), on muscular phase of experimental <i>Trichinella spiralis</i> infection in mice. Forty-eight Swiss albino mice were divided into 3 main groups: control, prophylactic, and therapeutic groups, with further subgrouping according to the received treatment. Larval count, inflammation around the encapsulated larvae, and oxidative stress markers were assessed. All groups receiving treatment showed a significant reduction in larvae count/gm muscle compared to the infected control group. In the prophylactic group, combined VitE-Se achieved the highest reduction, 58.4% and 51% in the diaphragm and intercostals, respectively. Whereas in the therapeutic group combined VitE-Se-ALB showed the maximum reduction rates of 90.4% and 84.4% in the diaphragm and intercostals, respectively. Varying grades of cellular inflammatory infiltration were detected around the capsule in various groups, with marked reduction in degree of inflammation in all combination groups containing ALB. Oxidative stress markers malondialdehyde (MDA) and superoxide dismutase (SOD) levels showed a significant decrease in treated muscle tissues, the lowermost level was detected in the VitE-Se-ALB combination group. In conclusion, combined Vit E-Se reduced muscle larval burden in the prophylactic group, potentiated the antiparasitic action of ALB in the therapeutic group, and demonstrated anti-inflammatory and antioxidant effects.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 2","pages":"378-388"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Loida M Recopuerto-Medina, Regina Rebecca A Atienza, Jorienne Mae F Flaminiano, Margaret M Manuel, Gia Mikaela T Perez, Corky Philip M Zeta, Francis Kristin Nicole R Medina, Nikki Heherson A Dagamac
{"title":"Geospatial analysis of <i>Aedes poicilius</i>, vector of Bancroftian Filariasis in the Philippines.","authors":"Loida M Recopuerto-Medina, Regina Rebecca A Atienza, Jorienne Mae F Flaminiano, Margaret M Manuel, Gia Mikaela T Perez, Corky Philip M Zeta, Francis Kristin Nicole R Medina, Nikki Heherson A Dagamac","doi":"10.1007/s12639-024-01766-z","DOIUrl":"10.1007/s12639-024-01766-z","url":null,"abstract":"<p><p>Bancroftian filariasis, one of the Philippines' neglected tropical diseases, is a parasitic disease caused by <i>Wuchereria bancrofti</i> and transmitted by <i>Aedes poicilius</i>, which thrive in the <i>Musa</i> plantations abundant in certain Philippine regions. Eliminating this disease is far from being achieved, thus emphasizing the need for a better control or elimination program by constructing a contemporary predictive model of the mosquito, <i>A. poicilius</i>, and identifying key environmental variables that favor the mosquito species. Modeling of the distribution of lymphatic filariasis was divided into two phases: data collection of disease occurrences and environmental variables from 1985 to 2019 and model calibration and testing utilizing the MaxEnt algorithm. Model sensitivity was validated through the area under the curve (AUC) method. The model had a mean training AUC of 0.995 ± 0.001. The Jackknife test was performed to determine the effect of the assessed variables on the prevalence of the disease and revealed that isothermality has the highest gain when used in isolation. The total frequency of lymphatic filariasis was mapped using the QGIS software to exhibit the suitability of agricultural plantations as breeding grounds for <i>A. poicilius</i> populations.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 2","pages":"407-418"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marwa M Attia, Amira M Ibrahim, Osama G Sakr, Heba M Salem, Sara S Barsoum, Mohamed Kamel, Sohila M El Gameel
{"title":"Health status of rabbits infested with sarcoptic mange with different lesion scores.","authors":"Marwa M Attia, Amira M Ibrahim, Osama G Sakr, Heba M Salem, Sara S Barsoum, Mohamed Kamel, Sohila M El Gameel","doi":"10.1007/s12639-024-01750-7","DOIUrl":"10.1007/s12639-024-01750-7","url":null,"abstract":"<p><p>This study aimed to evaluate the health status of rabbits with <i>Sarcoptes scabiei</i> (<i>S. scabiei</i>) lesions scores. From August 2022 to August 2023, 200 domestic rabbits were thoroughly inspected for the presence of mange in their bodies. Lesion scores were recorded; moreover, sera were collected for biochemical analysis, along with infested skin and spleen samples from the infested animals. The clinical skin lesions were present around the head, ears, neck, trunk, feet, and genitalia, which determined the lesion score. The skin lesion scores on the external ear margins, head, nose, and toes were indexed for 5 scores. Infested and un-infested control rabbits were divided into two groups of 10 each according to lesion score, and blood samples were collected from the ear vein for five milliliters of blood. Serum nitric oxide (NOx) and Major histocompatibility class I (MHC-I) were evaluated. The affected rabbits suffered from malnutrition and emaciation. There were also indications of scratching, itching, fur loss, hyperemia, and thickening of the skin with crust formation. The mite specimens in our study were identified as <i>S. scabiei</i> based on the ITS2 gene sequence submitted to GenBank (Accession No. PQ130162). The results of the phylogenetic analysis based on the ITS2 were performed using MEGA 7 software. Serum nitric oxide was evaluated according to the lesion scores; a score of 0 was negative healthy, and then the serum nitric oxide levels were elevated in accordance with the increase in the severity of the diseases as indicated by the lesion scores. There were measurable quantities of major histocompatibility class (MHC)-I mRNA that was examined in each group lesion score, either in the skin or the spleen. The spleen expression profile was higher in all groups than in the skin. With increasing of the score lesion the transcript levels increase.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 2","pages":"334-342"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A retrospective perspective on epidemiology, clinicopathological and therapeutic aspects of microfilariosis in buffaloes of West Godavari District, Andhra Pradesh.","authors":"K V Ramakrishna, P Ramadevi","doi":"10.1007/s12639-024-01774-z","DOIUrl":"10.1007/s12639-024-01774-z","url":null,"abstract":"<p><p>Utilizing retrospective data collected over a period of nine years during the period from 2010 till 2019, an attempt was made to study various aspects of microfileremia in adult buffaloes in West Godavari district of Andhra Pradesh. This study involves an analysis of epidemiological aspects of microfilariosis, clinical presentation and therapeutic efficacy of Ivermectin. The study involved initial examination of 15,851 blood samples of buffaloes collected in the district from different sources and various predisposing factors influencing the incidence like season, stocking density, vector propagation, individual and herd stressors, age and lactation status were considered. Diagnostic specificity and applicability of available tests like wet blood smear examination, methylene blue staining, Giemsa's staining and Modified Knott's test were evaluated. The incidence was highest during July-September (6.6%) followed by October-December (6.1%), January-March (5.2%) and April-June (4.9%). Post monsoon warm and humid weather appears to favour the vector propagation and incidence. High proportion of microfilaremia at least 2-3 per alternate microscopic fields was presumed to be responsible for clinical microfilariosis than low infection like one motile parasite for every 3-6 microscope fields. Fourteen (14) clinical cases and equal number of healthy control animals, both are lactating, calved 4-5 month back, with an average milk yield of 6.8 L were selected for clinical studies and evaluation of hematological and biochemical parameters. Therapeutic efficacy of Ivermectin was evaluated by assessing the circulatory clearance rate of microfilaria, improvement in clinical signs and return to productivity. The possibility of spontaneous elimination of parasite without specific treatment was not considered with an aim for rapid recovery without complications. Supportive therapy aided in the correction of anemia and speedy recovery. Recurrence of microfileremia was not observed after two months of periodic post therapeutic evaluation and follow-up.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"49 2","pages":"465-471"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}