Journal of Parasitic Diseases最新文献

{"title":"Infection of Egyptian domestic rabbits, Oryctolagus cuniculus, with Cysticercus pisiformis (Cestoda: Taeniidae): morphological, molecular, and histopathological diagnostic tools.","authors":"Soheir A H Rabie, Wafaa A Abuelwafa, Mouchira M Mohi Eldin, Nermean M Hussein","doi":"10.1007/s12639-024-01699-7","DOIUrl":"10.1007/s12639-024-01699-7","url":null,"abstract":"<p><p>Cysticercosis raises the alarm for domestic rabbits since it has a significant impact on their health, productivity, and nutritional value. In this study, several white creamy- colored cysts were detected in the mesentery of the stomach and intestine, as well as embedded in the liver of domestic rabbits collected from Qena Governorate, Egypt. Based on both morphological and molecular assessments, revealed cysts could be identified as <i>Cysticercus pisiformis</i>, the larval stage of <i>Taenia pisiformis</i>. Gross and histopathological investigations of infected organs were documented. To our knowledge, this is the first geographical report on <i>Cysticercus pisiformis</i> in domestic rabbits in Qena Governorate, using current morphological, molecular, and histopathological methodologies that provide critical data for developing diagnostic targets for <i>C. pisiformis</i>.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"810-822"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemoparasitism in grazing cattle and risk factors associated with husbandry management in an endemic area of Eastern Colombia.","authors":"Natalie Hell Mor, Julieth Viviana Montenegro Tavera, Julio César Tobón, Blanca Lisseth Guzmán Barragán, Giovanny Beltran López, Jimmy Jolman Vargas Duarte, Danny Wilson Sanjuanelo Corredor, Gabriel Andrés Tafur-Gómez","doi":"10.1007/s12639-024-01723-w","DOIUrl":"10.1007/s12639-024-01723-w","url":null,"abstract":"<p><p>Vector-borne pathogens induce hemoparasitism in cattle causing substantial economic losses in tropical and subtropical areas. Infectious cattle actively contribute to maintaining the transmission cycle, and the presence of these animals must be associated with husbandry management and environmental changes. In the present study, we conducted a cross-sectional study sampling 1,000 bovines to identify infectious cattle diagnosed by a direct technique and employed a dichotomic questionnaire for association analyses, hierarchical clustering, and Principal Component Analysis (PCA). Overall prevalence with infectious cattle was 34.99%, where 97% of the farms had at least one infectious animal per genera, and the prevalence in properties ranged between 16.39 and 53.85%. Of these animals, 26.20% tested positive for <i>Anaplasma sp.</i>, 8.40% for <i>Babesia</i> spp., and 1.30% for <i>Trypanosome</i> spp. The main co-infection showed 5% <i>Anaplasma</i> sp. <i>- Trypanosome</i> spp., followed by 4% <i>Babesia</i> spp. - <i>Trypanosome</i> spp. These bovines showed association with the use of the Jersey breed (OR = 2.016 C.I:1.188-3.419), selling animals for replacement (OR = 1.417 CI:1.022-1.965), participation in livestock exhibitions (OR = 2.009 CI:1.262-3.199), premises with burials (OR = 2,064 CR: 1.414-3.011), use of palm kernel (OR = 1.935 C.I:1.198-3.124), and the use of ivermectin (OR = 1.548 CI: 1.085-2.210) as a susceptibility. The hierarchical clustering revealed clusters among properties with different hemoparasite prevalence, with notable co-infections observed. The subsequent PCA identified that significant risk factors contributed to hemoparasitism positivity. We conclude that infectious cattle in the endemic area showed an association with husbandry management that permits the success of vector and maintenance of the enzootic or epizootic cycle in the herds.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"924-935"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Antileishmanial effect of podophyllotoxin and podophyllin on <i>Leishmania major</i> in vitro and in vivo.","authors":"Amir Hossein Ghayour, Mahdi Delavari, Mohsen Arbabi","doi":"10.1007/s12639-024-01719-6","DOIUrl":"https://doi.org/10.1007/s12639-024-01719-6","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1007/s12639-024-01654-6.].</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"959-960"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of platelet rich plasma against experimental <i>Cryptosporidium parvum</i> infection: in vivo study in immunosuppressed mice.","authors":"Nada R Mahmoud, Azza I Younis, Rabab S Zalat, Ahmed S A Soliman, Mona M Khater","doi":"10.1007/s12639-024-01713-y","DOIUrl":"10.1007/s12639-024-01713-y","url":null,"abstract":"<p><p>The present study was designed to assess the possible effects of platelet rich plasma (PRP) when used individually and in combination with nitazoxanide (NTZ) on experimental <i>Cryptosporidium parvum</i> (<i>C. parvum</i>) infection. It was conducted on 100 male albino mice, laboratory bred in Theodore Bilharz Research Institute. Starting from the 7th day post infection (p.i), therapeutics were given to immunosuppressed infected mice, which were divided as follows; oral NTZ treated group (0.2 mg/g/day for 6 consecutive days), six-PRP-treated groups (0.5 μl/g/week) to be administered intravenously (IV) in 1st, 2nd, 3rd week as PRP alone in (3 groups) and combined with oral NTZ (0.2 mg/g/day for 6 consecutive days) in (3 groups). Parasitological, histopathological and immunohistochemical assessments of therapeutics under study were done. Fecal pellets collected from groups at different intervals were stained using modified Ziehl-Neelsen and examined under microscope. Among PRP-treated groups, the highest significant percentage of oocyst reduction (89.96%) was observed in the group received 3 doses of PRP in combination with NTZ on the 35th day post infection. Likewise, the histopathological examination of small intestinal tissue sections showed improvement in villous architecture with mild to moderate stunting and moderate inflammatory infiltrates in lamina propria. Immunohistochemical staining of small intestinal tissue sections showed moderate increase in the expression of TGF-β1. Therefore, PRP can be a novel strategy in the treatment of cryptosporidiosis particularly when combined with NTZ.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"849-859"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthelmintic activity of crude and separated extract of <i>Aloe vera</i> (Xanthorrhoeaceae) against bovine adults parasites of <i>Onchocerca ochengi</i> and infected larvae of drug resistant strains of the free-living nematode <i>Caenorhabditis elegans</i>.","authors":"Justin Kalmobe, Jacqueline Dikti Vildina, Djafsia Boursou, Honore Ndouwe Tissebe Menga, Simeon Fogue Kouam, Dieudonne Ndjonka","doi":"10.1007/s12639-024-01701-2","DOIUrl":"10.1007/s12639-024-01701-2","url":null,"abstract":"<p><p>Human onchocerciasis is caused by the filarial worm. <i>Onchocerca volvulus</i> is a parasite that forms nodules under the skin. The aim of this work was to assess the nematicidal activities of <i>Aloe vera</i> on <i>Onchocerca ochengi</i> and <i>Caenorahbditis elegans</i> and to determine the phytochemical compounds. Nodules were collected from the umbilical region of infected cattle, dissected and male worms were cultured in RPMI-1640. Worms were incubated with different concentrations of <i>A. vera</i> extracts in RPMI-1640 and M9-buffer. Polyphenol, tannin and flavonoid contents of extract were determined by using gallic acid and rutin as standards. The anthelmintic effect of <i>A. vera</i> extract against <i>O. ochengi</i> was concentration dependent with LC₅₀ of 20.71 µg/mL and 11.75 µg/mL after 48 and 72 h respectively. <i>A. vera</i> extract exerted concentration dependent lethal effects (LC₅₀ = 2747 and LC₅₀ = 31,937 µg/mL) against <i>C. elegans</i> (Wild Type). Methanolic-methylene chloride (MeOH-CH₂Cl₂) of <i>A. vera</i> extract exhibited high DPPH activity with an IC₅₀ value of 15 µg/mL and 9 µg/mL for ascorbic acid. The highest activity in adult worms was observed with the MeOH (100: 0) and AcOEtMeOH fractions with LC₅₀ values of 12.82 and 15.50 µg/mL respectively. EcOEtMeOH (8:2 v/v) was more effective (LC₅₀ = 250 µg/mL) on WT of <i>C. elegans. A. vera</i> contains polyphenols (1015.05 and AcOEtMeOH = 893.60), flavonoids (25.35 and MeOH = 225.76) and tannins (401.37 and Hex = 788.89). <i>A. vera</i> showed in vitro nematicidal activity against <i>O. ochengi</i> and <i>C. elegans</i>. <i>A. vera</i> could be used as an alternative anthelmintic for onchocerciasis treatment.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"891-903"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Schistosoma mansoni</i> infection and hepatocellular carcinoma: a comorbidity study.","authors":"Amal Farahat Allam, Hoda Fahmy Farag, Amel Youssef Shehab, Ahmed Soliman El Sahy, Safia Saleh Khalil, Naglaa Fathi Abd El-Latif","doi":"10.1007/s12639-024-01721-y","DOIUrl":"10.1007/s12639-024-01721-y","url":null,"abstract":"<p><strong>Background: </strong>The implication of human <i>Schistosoma mansoni</i> (<i>S. mansoni</i>) infection in concomitance with other risk factors such as hepatitis C virus (HCV) and hepatitis B virus (HBV) in the development of hepatocellular carcinoma (HCC) is still under controversy. This work aimed. to evaluate the role of <i>S. mansoni</i> infection in association with hepatitis B virus (HBV), hepatitis C virus (HCV) and other risk factors in the development and/or progress of HCC.</p><p><strong>Methods: </strong>The present study was carried out on 90 HCC patients recruited from Kafr El-Sheikh Liver Disease Research Institute. After obtaining their informed consents, socio-demographic and clinical data were collected and patients were examined for <i>S. mansoni</i> by Kato-Katz and indirect hemagglutination (IHA) techniques. Alpha-fetoprotein (AFP) level was determined. The Child-Pugh scoring system and Barcelona Clinic Liver Cancer (BCLC) staging system were used to evaluate the pathological features of the studied patients.</p><p><strong>Results: </strong>All participants were negative for active <i>S. mansoni</i> by Kato-Katz. Based on IHA, the participants were categorized into two groups: group I: sixty-two patients negative for <i>S. mansoni</i> and group II: twenty-eight schistosomiasis positive. The patients' age ranged between 40->60, HCC was more prevalent in the age range of > 50-60 years in both groups. Males were more than females and rural participants were more than urban patients in both groups. Most of the patients (88.9%) had HCV while 7.8% had HBV. A higher proportion of HCC patients showed concomitant HCV and <i>S. mansoni</i> (92.6%) than the <i>S. mansoni</i> negative group. The frequency of upper gastrointestinal bleeding (GIB) was four-fold higher among HCC patients positive for schistosomiasis compared to negative schistosomiasis cases (64% vs. 16%). Alpha-fetoprotein (AFP) level was higher in group II than that in group I with no significant difference. Statistical analysis showed no difference between the two studied groups regarding Child scores. On the contrary, BCLC class D was significantly higher among HCC positive schistosomiasis cases compared to the negative group.</p><p><strong>Conclusion: </strong>Concomitant <i>S. mansoni</i> with HCV and HBV potentiate HCC progression.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"936-943"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Burden, knowledge and perception of lymphatic filariasis in resource - poor communities in north central Nigeria.","authors":"E C Amaechi, A A Ariyo, A A Aderogba, P C Nwachukwu, C C Nwadike, O O Ezekiel, F O P Oyinloye","doi":"10.1007/s12639-024-01711-0","DOIUrl":"10.1007/s12639-024-01711-0","url":null,"abstract":"<p><p>A number of vector-borne diseases are known to affect humans in tropical and sub-tropical regions of the world. Lymphatic filariasis is one of such debilitating disease caused by a nematode, <i>Wuchereria bancrofti.</i> The present study assessed the prevalence of lymphatic filariasis by screening individuals with filariasis test strip and clinical examination. A sample of 765 consenting individuals were screened for filarial antigenemia using filariasis test strip and clinical examination and interviewed for knowledge and perception of filariasis using a structured questionnaire. Overall antigenemia prevalence was 8.8% and the disease prevalence varied among villages with Shonga (16.7%) having the highest and Tada (1.6%) the least infected.In relation to age group, 40-49years had the highest (49.3%) while 0-9 years did not record any infection. Among the respondents infected, a greater number (43.3%) believed that the disease was caused by stepping on charm, 45.4% of those unaffected also believed in the same cause. A greater number of the respondents (28.4%) believed that avoiding sexual intercourse with affected individuals can help prevent the disease. A majority(67.2%) of the study participant had a misconception about its preventive measure. Misconceptions on the preventive measures included sacrifice to appease the gods and avoiding sexual intercourse with affected individuals. This study showed filarial antigenemia was above the threshold of 2% in the study area with misconception on the disease transmission and therefore this area warrants strategies to prevent transmission.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 4","pages":"823-830"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of <i>Anaplasma, Babesia, Theileria</i>, and <i>Trypanosoma</i> infection in cattle and buffaloes in India.","authors":"Syeda Afshan Fatima, Hariprasad Naidu Gonuguntla, Ponnanna Nadikerianda Muthappa, Laxmi Narayan Sarangi","doi":"10.1007/s12639-024-01673-3","DOIUrl":"10.1007/s12639-024-01673-3","url":null,"abstract":"<p><p>Tick-borne pathogens pose a significant global threat, causing substantial economic losses to the dairy industry. In India, tropical theileriosis, anaplasmosis, babesiosis, and trypanosomiasis are major hemo-parasitic diseases affecting bovines. A cross-sectional study was conducted to determine the prevalence of hemo-parasites in different farms in India. PCR assays were employed to detect carrier status, using gene targets <i>msp1b, tams1, rap-1, ama1,</i> and <i>ITS1</i> for <i>A. marginale, T. annulata, B. bovis, B. bigemina,</i> and <i>Trypanosoma</i> species, respectively. Out of the 578 apparently healthy animals screened, 30.45% (95% CI: 26.84-34.32%) were infected with at least one hemo-parasite. Cattle showed an overall positivity of 32.87%, while buffaloes had a prevalence of 15.19%, which was statistically significant (<i>p</i> < 0.001). Interestingly, prevalence was higher in indigenous cattle (47.81%) compared to cross-breeds (25.53%) and exotics (14.62%), with a statistically significant difference (<i>p</i> < 0.001). The prevalence of hemo-parasites varied widely among the farms, ranging from 5.77 to 100%. <i>A. marginale</i> was the most prevalent parasite (23.70% of animals), followed by <i>T. annulata</i> (13.67%), <i>Babesia</i> species (1.90%), and <i>Trypanosoma</i> species (1.56%). Enzootic instability was observed in six of the eight farms, indicating a potential for future outbreaks. Co-infection was detected in 60 out of 176 animals positive for hemo-parasites, with 59 animals co-infected with <i>A. marginale</i> and <i>T. annulata</i>, and only one cross-breed cattle infected with both <i>Anaplasma marginale</i> and <i>Babesia bigemina</i>. The findings highlight the prevalence of hemo-parasites in farms, underscoring the need for whole-herd screening, treatment of infected animals, and improvement in farm management practices to prevent production losses caused by these pathogens.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12639-024-01673-3.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 3","pages":"450-459"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141982537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of water quality and morphometric indices on the spatio-temporal prevalence of fish endo-parasites and diversity in the Ase River, Niger-Delta, Nigeria.","authors":"Edore Edwin Ito, Andy Ogochukwu Egwunyenga","doi":"10.1007/s12639-024-01668-0","DOIUrl":"10.1007/s12639-024-01668-0","url":null,"abstract":"<p><p>Three stations along the Ase River, Delta State Nigeria provided water and 85 fish samples which were analyzed. The fish were measured and examined for endoparasites according to established protocol. All water quality parameters investigated were within the WHO-acceptable values for surface waters. Station variation of physicochemical parameters was not statistically significant (<i>p</i> > 0.05). Fish body conformation indices positively correlated with the prevalence of parasites in <i>Clarias gariepinus, Heterobranchus longifilis, Parachana africana, Chromidotilapia guntheri guntherii</i>, and <i>Denticeps clupeodes</i>. The overall parasite prevalence of 63.53% was established with the most abundant parasite being <i>Trichodina mutabillis</i>. The parasites had a predilection for the gastrointestinal tract with a high occurrence of 307 individuals. Stations 1, 2, and 3 had 326, 213, and 259 parasites, respectively, out of a total of 798 parasites detected. <i>P. laevis</i> was absent in station 1. All parasites were found in stations 2 and 3. Statistically, there was a significant difference (<i>p</i> < 0.05) in the prevalence in all stations. The correlation index of <i>T. mutabillis</i> and <i>R. congolensis</i> in stations 1 and 3 was positively strong (<i>p</i> < 0.05) with the concentrations of water quality. However, water conditions in stations 1 and 3 had a deleterious impact on <i>P. laevis</i>. <i>T. mutabillis</i> maintained a high positive correlation with physicochemical water quality in all three stations. Shannon-Weiner's index in station 3 (H = 1.337) shows that the parasites were more diverse. PCA and biodiversity indices have enabled us to comprehend how parasite-host-environment systems interact.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 3","pages":"439-449"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141982506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of <i>Microphallus</i> sp. (Digenea: Microphallidae) parasitic in freshwater crabs of Manipur, India.","authors":"Voleentina Devi Athokpam, Lalit Mohan Goswami, Veena Tandon","doi":"10.1007/s12639-024-01694-y","DOIUrl":"10.1007/s12639-024-01694-y","url":null,"abstract":"<p><p>Freshwater crabs (<i>Potamiscus manipuriensis</i>), commonly consumed as local delicacies by the native people in the state of Manipur, were found to harbour metacercariae of <i>Microphallus</i> sp. (Family Microphyllidae), which were morphologically different from metacercariae of <i>Microphallus</i> spp reported earlier from different regions. So, PCR-based molecular characterization of this metacercaria was done utilizing rDNA marker regions: larger subunit (LSU) or 28S (D1-D3 region) and inter-transcribed spacer 2 (ITS2). Sequence and phylogenetic analyses confirmed that the taxon under study belonged to family Microphyllidae of genus <i>Microphallus</i>.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 3","pages":"570-580"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141982507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}