Journal of Orthopaedic Research®最新文献_第6页

Evaluation of conventional and CT-based radiostereometric analysis for inducible displacement measurements after total hip arthroplasty 评估全髋关节置换术后用于诱发位移测量的传统和基于 CT 的放射立体测量分析。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-10-01 DOI: 10.1002/jor.25981

Jennifer S. Polus, Bart L. Kaptein, Edward M. Vasarhelyi, Brent A. Lanting, Matthew G. Teeter

{"title":"Evaluation of conventional and CT-based radiostereometric analysis for inducible displacement measurements after total hip arthroplasty","authors":"Jennifer S. Polus, Bart L. Kaptein, Edward M. Vasarhelyi, Brent A. Lanting, Matthew G. Teeter","doi":"10.1002/jor.25981","DOIUrl":"10.1002/jor.25981","url":null,"abstract":"Though radiostereometric analysis (RSA) is the gold standard for migration tracking, computed tomography-based RSA (CT-RSA) does not require marker beads and is available for clinical adoption. This study investigated CT-RSA in comparison to RSA for assessing hip implant stability with inducible displacement (ID) examinations. Patients (n = 48) from a previous study returned to be re-examined for femoral stem stability with CT-RSA and RSA imaging. Implant migration since patients last follow-up was calculated as a measure of stability. ID was assessed between alternated leg rotation scans for CT-RSA and between supine and weight-bearing scans for RSA. Measurements from ID and double examinations were compared between CT-RSA and RSA. All stems were well-fixed with migration <0.2 mm/year. ID measurements were lower with CT-RSA than RSA for distal translation (mean difference = 0.122 mm, p < 0.0001), total translation (mean difference = 0.158 mm, p < 0.0001), and total rotation (mean difference = 0.449°, p < 0.0001). The ID and double exam were significantly different for total translation and total rotation for CT-RSA, and significantly different for medial, distal, and total translation, and total rotation for RSA. Precision ranged from 0.049 to 0.130 mm in translation and 0.061° to 0.220° in rotation for CT-RSA, and from 0.108 to 0.269 mm in translation and 0.151° to 0.670° in rotation for RSA. ID measurements from both CT-RSA and RSA were minimal, consistent for a cohort with well-fixed stems. CT-RSA demonstrated superior precision in all axes compared to RSA. Clinical Significance: Future work should explore the use of CT-RSA in patients with suspected loosening as a potential diagnostic tool.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 1","pages":"192-199"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25981","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cross-sectional morphology of the proximal femoral diaphysis is defined by the anteversion angle. 股骨近端骨骺的横截面形态由前倾角决定。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-30 DOI: 10.1002/jor.25983

Daisuke Endo, Keita Nishi, Takeshi Imamura, Kazunobu Saiki, Keiko Ogami-Takamura, Kiyohito Murai, Toshiyuki Tsurumoto

{"title":"The cross-sectional morphology of the proximal femoral diaphysis is defined by the anteversion angle.","authors":"Daisuke Endo, Keita Nishi, Takeshi Imamura, Kazunobu Saiki, Keiko Ogami-Takamura, Kiyohito Murai, Toshiyuki Tsurumoto","doi":"10.1002/jor.25983","DOIUrl":"https://doi.org/10.1002/jor.25983","url":null,"abstract":"Osteoporosis in postmenopausal women is one of the causes of femoral fractures and is prevented by the administration of bisphosphonates. Individual morphologies are considered to increase the risk of atypical fractures associated with long-term administration. To evaluate cortical bone morphology quantitatively, we established a method to measure the distance from the center point of a cross-section to the external and internal borders based on CT images. Using this method, 44 sides of a female femoral skeleton specimen were examined and areas of protrusion and thickening in the medial anterior and lateral posterior regions just below the lesser trochanter were identified. These positions strongly correlated with the anteversion angle, suggesting the involvement of the distribution of the load received from body weight defined by the angle. The finite element method was used to examine the relationships between the positions of these areas with compressive and tensile stress distribution areas in the one-legged standing condition. The medial anterior region and lateral posterior region protruded and thickened in response to compressive and tensile stress, respectively. In addition, a hierarchical relationship was observed between the anteversion angle, tensile stress distribution, protrusion, and thickening in femurs with thinning of cortical bone, indicating that morphogenesis occurs adaptively to loading. The present results demonstrate the usefulness of this method in considering the formation mechanism and function of the femoral diaphysis and suggest that bone remodeling is necessary to maintain adaptability.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cervical motion analysis using wearable inertial sensors to patients with cervical ossification of posterior longitudinal ligament. 使用可穿戴惯性传感器对颈椎后纵韧带骨化患者进行颈椎运动分析。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-29 DOI: 10.1002/jor.25985

Satoshi Osuka, Masahiko Takahata, Ryo Takeda, Takeshi Chiba, Hiroaki Hori, Yoshiaki Kataoka, Norimasa Iwasaki, Masahiko Mukaino, Harukazu Tohyama

{"title":"Cervical motion analysis using wearable inertial sensors to patients with cervical ossification of posterior longitudinal ligament.","authors":"Satoshi Osuka, Masahiko Takahata, Ryo Takeda, Takeshi Chiba, Hiroaki Hori, Yoshiaki Kataoka, Norimasa Iwasaki, Masahiko Mukaino, Harukazu Tohyama","doi":"10.1002/jor.25985","DOIUrl":"https://doi.org/10.1002/jor.25985","url":null,"abstract":"This study aimed to clarify cervical kinematics during daily activities, including level walking and stair ascending, in patients with cervical ossification of the posterior longitudinal ligament (C-OPLL). Eighteen patients with myelopathy caused by C-OPLL and 18 healthy controls were recruited to participate in the study. The sagittal cervical kinematics during level walking and stair ascent were quantitatively assessed using a motion analysis system based on wearable inertial sensors. The Japanese Orthopaedic Association score, Japanese Orthopaedic Association Cervical Myelopathy Evaluation Questionnaire, Neck Disability Index, and deep sensation in the lower extremities were assessed in all participants. Nine of 18 patients with C-OPLL presented with deep sensory disturbances. Patients with C-OPLL with deep sensory disturbances exhibited different sagittal plane cervical motion patterns than healthy controls during level walking and stair ascent. During the first phase of stair ascent, both patients with C-OPLL and healthy controls flexed their necks to the same degree; however, during the middle and final phases of stair ascent and all phases of level walking, the mean cervical flexion angle of patients with C-OPLL with deep sensory disturbances was significantly higher than that of patients with C-OPLL without deep sensory disturbance and healthy controls. Our data suggest that patients with C-OPLL presenting with deep sensory disturbances are likely to walk with their necks flexed and gaze downward to observe their steps throughout their daily lives. This habitual neck posture may lead to a vicious cycle of cervical kyphosis and worsening of compressive myelopathy.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal high-fat high-sugar diet impairs bone quality and strength but not cartilage in aging mice 母体高脂高糖饮食会损害衰老小鼠的骨骼质量和强度，但不会损害软骨。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-29 DOI: 10.1002/jor.25980

Arin K. Oestreich, Natalia S. Harasymowicz, Alireza Savadipour, Zainab Harissa, Neda Rashidi, Meredith K. Luhmann, Mohammed Kuziez, Kelle H. Moley, Farshid Guilak

{"title":"Maternal high-fat high-sugar diet impairs bone quality and strength but not cartilage in aging mice","authors":"Arin K. Oestreich, Natalia S. Harasymowicz, Alireza Savadipour, Zainab Harissa, Neda Rashidi, Meredith K. Luhmann, Mohammed Kuziez, Kelle H. Moley, Farshid Guilak","doi":"10.1002/jor.25980","DOIUrl":"10.1002/jor.25980","url":null,"abstract":"Osteoarthritis (OA) is a prevalent aging disorder of synovial joints and recent work suggests that a parental high-fat diet increases OA severity following joint injury in offspring. We hypothesized that a maternal high-fat high-sugar (HFHS) diet would promote spontaneous osteoarthritis-related cartilage and bone changes in 1-year-old offspring. Female C57BL/6 J mice were placed on either a chow control or HFHS diet for 6 weeks before mating to a chow-fed C57BL/6 J male and maintained on their assigned diets throughout pregnancy and lactation. Male and female offspring were weaned onto a chow diet, raised to 1 year of age, and evaluated for cartilage and bone changes indicative of OA. However, offspring did not show early signs of OA as measured by histological Mankin scoring, mechanical testing of the pericellular matrix, histological synovitis scoring, or subchondral bone thickening as measured by microcomputed Tomography. On the other hand, male offspring from HFHS-fed dams had reduced trabecular bone quality in the tibial metaphysis and decreased cortical thickness. Although maternal HFHS diet did not impact trabecular or cortical bone quality in tibias of female offspring, the radii of these animals had decreased cortical thickness, increased medullary area, and impaired breaking strength compared to those of control-fed dams. Finally, we evaluated bone quality and strength in male and female F2 offspring and found that the grandmaternal diet modestly impacted radial bone geometry but not strength. Together these results suggest that maternal HFHS diet impairs F1 offspring skeletal integrity in a sex and bone site-specific manner.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 1","pages":"117-127"},"PeriodicalIF":2.1,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell migration within porous electrospun nanofibrous scaffolds in a mouse subcuticular implantation model 小鼠皮下植入模型中多孔电纺纳米纤维支架内的细胞迁移。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-29 DOI: 10.1002/jor.25979

Waleed Mohammad, Liang Chen, Bin Wu, Paula Dietz, Therese Bou-Akl, Weiping Ren, David C. Markel

{"title":"Cell migration within porous electrospun nanofibrous scaffolds in a mouse subcuticular implantation model","authors":"Waleed Mohammad, Liang Chen, Bin Wu, Paula Dietz, Therese Bou-Akl, Weiping Ren, David C. Markel","doi":"10.1002/jor.25979","DOIUrl":"10.1002/jor.25979","url":null,"abstract":"Cellular infiltration into electrospun nanofibers (NFs) is limited due to the dense structure and small pore sizes. We developed a programmed NF collector that can fabricate porous NFs with desired pore sizes and thickness. Previously we demonstrated improved cellular proliferation and differentiation of osteoblasts, osteoclasts, and fibroblasts with increased pore sizes of polycaprolactone (PCL) NF in-vitro. This study investigated in-vivo host cell migration and vascular ingrowth within porous NF sheets implanted subcutaneously in a mouse model. Two types of PCL NFs with well-defined pore sizes were created using varying speeds of the NF collector: NF-zero (no movement, pore size 14.4 ± 8.9 µm2) and NF-high (0.232 mm/min, pore size 286.7 ± 381.9 µm2). The NF obtained by using classical flat NF collector (2D NF, pore size 1.09 ± 1.7 µm2) was a control. The three formulae of NFs were implanted subcutaneously in 18 BALB/cJ mice. Animals were killed 7 and 28-days after implantation (n = 3 per group per time point). The tissue with implanted NFs were collected for histologic analysis. Overall, 7-day samples showed little inflammatory response. At 28-days, the degree of tissue penetration of PCL NF sheet matrices was linked to pore size and area. NFs with the largest pore area had more efficient tissue migration and new blood vessel formation compared to those with smaller pore sizes. No newly formed blood vessels were observed in the 2D NF group. A porous NF scaffold with controllable pore size has potential for tissue repair/regeneration in situ with potential for many applications in orthopaedics.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 1","pages":"153-160"},"PeriodicalIF":2.1,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An in vitro study of glenoid implant peripheral peg interface mechanics during eccentric loading and lift-off 偏心加载和抬起时髋臼假体外周钉界面力学的体外研究。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-25 DOI: 10.1002/jor.25976

Jakub Targosinski, Jonathan Kusins, George Athwal, Andrew Nelson, Louis Ferreira

{"title":"An in vitro study of glenoid implant peripheral peg interface mechanics during eccentric loading and lift-off","authors":"Jakub Targosinski, Jonathan Kusins, George Athwal, Andrew Nelson, Louis Ferreira","doi":"10.1002/jor.25976","DOIUrl":"10.1002/jor.25976","url":null,"abstract":"Glenoid implants used in anatomic total shoulder arthroplasties typically incorporate peripheral pegs as a design feature to support eccentric loads. These peripheral pegs and the implant-cement-bone interface undergo substantial cyclic tensile-compressive loads during normal activity. Therefore, these pegs are of interest in translating the micromechanics of local implant fixation failure to the biomechanics of gross anatomic failure of the glenoid implant after total shoulder arthroplasty. This study used an in vitro peg-cement-bone construct which incorporated bone tissue acquired from osteoarthritic patients undergoing total shoulder arthroplasty. Strain distributions in the peripheral peg-cement-bone interfaces were analyzed under loading conditions emulating glenoid implant edge displacements. It was found that tensile strains in the interfaces were highest near the backside-peg junction and were greater in magnitude than compressive strains. Notably, strains near the peg's fixation channels were relatively low. These results suggest that cracks may initiate around the peg near the backside and travel downward to cause broader fixation failure.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 1","pages":"228-237"},"PeriodicalIF":2.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25976","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of CGRP receptor attenuates tendon graft degeneration in anterior cruciate ligament reconstruction CGRP 受体的过度表达可减轻前十字韧带重建中肌腱移植物的退化。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-25 DOI: 10.1002/jor.25978

Xibang Zhao, Zhaoji Cai, Ying Luo, Zhousheng Lin, Jiali Wang

{"title":"Overexpression of CGRP receptor attenuates tendon graft degeneration in anterior cruciate ligament reconstruction","authors":"Xibang Zhao, Zhaoji Cai, Ying Luo, Zhousheng Lin, Jiali Wang","doi":"10.1002/jor.25978","DOIUrl":"10.1002/jor.25978","url":null,"abstract":"Cell apoptosis or necrosis, extracellular matrix loss, and excessive inflammation may induce tendon graft degeneration. The impairment in the regeneration capability of nerve fibers and blood vessels may be the critical cause. Calcitonin gene-related peptide (CGRP), exhibiting a short half-life, favors cell proliferation, nerve fiber regeneration and angiogenesis. We aimed to investigate the effects of CGRP receptor-mediated signaling on tendon graft integrity and study if the modulation pathways are ascribed to cell proliferation, nerve fiber and blood vessel regeneration. A total of three groups in mice with ACL reconstruction were established: the control group (PBS treatment), the adenovirus vectors expressing CGRP receptor (CALCRL) treated group (Adv-Calcrl treatment), and the adenovirus vectors carrying shRNA targeting Calcrl treated group (Adv-shCalcrl treatment). The histological assessment indicated the Adv-Calcrl treatment was favored while the Adv-shCalcrl significantly impaired tendon graft integrity. TUNEL staining revealed a significant decreased number of apoptotic cells in the Adv-Calcrl group relative to the control group and the adv-shCalcrl group. Compared to the control group and the Adv-shCalcrl group, the Adv-Calcrl group showed significantly enhanced proliferation of nestin positive cells. Of note, the Adv-Calcrl treatment significantly increased EMCN expression at the tendon graft relative to the control and the Adv-shCalcrl groups, which may be ascribed to attenuation of the Hippo signaling pathway. Importantly, the Adv-Calcrl treatment significantly increased sensory nerve fibers and also PIEZO2 levels. Our results demonstrate the activation of CGRP receptor-mediated signaling attenuated tendon graft degeneration, which was ascribed to enhanced proliferation of Nestin positive cells, angiogenesis, and nerve fiber outgrowth.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 1","pages":"37-45"},"PeriodicalIF":2.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of collagen content is localized near cartilage lesions on the day of injurious loading and intensified on day 12 胶原蛋白含量的损失主要集中在软骨损伤当天的损伤部位，并在第 12 天加剧。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-23 DOI: 10.1002/jor.25975

Moustafa Hamada, Atte S. A. Eskelinen, Cristina Florea, Santtu Mikkonen, Petteri Nieminen, Alan J. Grodzinsky, Petri Tanska, Rami K. Korhonen

{"title":"Loss of collagen content is localized near cartilage lesions on the day of injurious loading and intensified on day 12","authors":"Moustafa Hamada, Atte S. A. Eskelinen, Cristina Florea, Santtu Mikkonen, Petteri Nieminen, Alan J. Grodzinsky, Petri Tanska, Rami K. Korhonen","doi":"10.1002/jor.25975","DOIUrl":"10.1002/jor.25975","url":null,"abstract":"Joint injury can lead to articular cartilage damage, excessive inflammation, and post-traumatic osteoarthritis (PTOA). Collagen is an essential component for cartilage function, yet current literature has limited understanding of how biochemical and biomechanical factors contribute to collagen loss in injured cartilage. Our aim was to investigate spatially dependent changes in collagen content and collagen integrity of injured cartilage, with an explant model of early-stage PTOA. We subjected calf knee cartilage explants to combinations of injurious loading (INJ), interleukin-1α-challenge (IL) and physiological cyclic loading (CL). Using Fourier transform infrared microspectroscopy, collagen content (Amide I band) and collagen integrity (Amide II/1338 cm−1 ratio) were estimated on days 0 and 12 post-injury. We found that INJ led to lower collagen content near lesions compared to intact regions on day 0 (p < 0.001). On day 12, near-lesion collagen content was lower compared to day 0 (p < 0.05). Additionally, on day 12, INJ, IL, and INJ + IL groups exhibited lower collagen content along most of tissue depth compared to free-swelling control group (p < 0.05). CL groups showed higher collagen content along most of tissue depth compared to corresponding groups without CL (p < 0.05). Immunohistochemical analysis revealed higher MMP-1 and MMP-3 staining intensities localized within cell lacunae in INJ group compared to CTRL group on day 0. Our results suggest that INJ causes rapid loss of collagen content near lesions, which is intensified on day 12. Additionally, CL could mitigate the loss of collagen content at intact regions after 12 days.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 1","pages":"70-83"},"PeriodicalIF":2.1,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25975","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analgesic effects of co-administration of mirogabalin and diclofenac sodium on neuropathic pain in rats 米罗加巴林和双氯芬酸钠联合给药对大鼠神经性疼痛的镇痛作用

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-22 DOI: 10.1002/jor.25977

Hiroto Chikubu, Kazuhide Inage, Sumihisa Orita, Yasuhiro Shiga, Masahiro Inoue, Keisuke Shimizu, Miyako Suzuki-Narita, Ikuko Tajiri, Michiaki Mukai, Natsuko Nozaki-Taguchi, Seiji Ohtori

{"title":"Analgesic effects of co-administration of mirogabalin and diclofenac sodium on neuropathic pain in rats","authors":"Hiroto Chikubu, Kazuhide Inage, Sumihisa Orita, Yasuhiro Shiga, Masahiro Inoue, Keisuke Shimizu, Miyako Suzuki-Narita, Ikuko Tajiri, Michiaki Mukai, Natsuko Nozaki-Taguchi, Seiji Ohtori","doi":"10.1002/jor.25977","DOIUrl":"10.1002/jor.25977","url":null,"abstract":"Co-administration of mirogabalin besylate and nonsteroidal anti-inflammatory drugs is effective for neuropathic pain; however, mechanism of its action remains unknown. We aimed to evaluate the mechanism of this synergistic effect of the concomitant administration for neuropathic pain using chronic constriction injury model rats. Fifty male Wister rats of 7-week-old were used. Right sciatic nerve ligation was performed in 40 rats and they were sub-divided into four groups: vehicle, mirogabalin, diclofenac sodium and co-administration of them. Ten rats underwent sham surgery. Fluorogold was attached to sciatic nerve during surgery. Von Frey filament and weight bearing tests were performed on postoperative Day 6 as behavioral assessments and drug was administrated intraperitoneally. Half rats in each group underwent behavioral assessment and perfusion fixation using 4% paraformaldehyde on postoperative Day 7 and remaining on postoperative Day 14. Subsequently, dorsal root ganglion at L4 to L6 was collected and examined immunohistochemistry for calcitonin gene-related peptide, and their immunoreactivity in fluorogold-labeled neurons was measured. Spinal cord at lumbar swelling was resected, immunostained for ionized-calcium-binding adapter molecule-1 and glial fibrillary acidic protein, and immunoreactive neurons in dorsal horn of spinal cords were calculated as the occupancy of them. Mirogabalin suppresses the neuropeptide-release from presynaptic afferent neuron directly and it resulted in suppressing glia cells activation. Diclofenac sodium inhibits cyclooxygenase-2 and prostaglandin production, related to allodynia. These effects of mirogabalin and diclofenac sodium, respectively, inhibited glia cells strongly, which is presumed to be one of the mechanisms for the effectiveness of their co-administration for neuropathic pain.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 1","pages":"200-209"},"PeriodicalIF":2.1,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A hybrid repair strategy for full-thickness cartilage defects: Long-term experimental study in eight horses 全厚软骨缺损的混合修复策略：八匹马的长期实验研究

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-18 DOI: 10.1002/jor.25972

Maria C. Fugazzola, Mylène De Ruijter, Stefanie Veraa, Saskia Plomp, Ward van Buul, Gied Hermsen, René van Weeren

{"title":"A hybrid repair strategy for full-thickness cartilage defects: Long-term experimental study in eight horses","authors":"Maria C. Fugazzola, Mylène De Ruijter, Stefanie Veraa, Saskia Plomp, Ward van Buul, Gied Hermsen, René van Weeren","doi":"10.1002/jor.25972","DOIUrl":"10.1002/jor.25972","url":null,"abstract":"The objective of this study was to evaluate a non-resorbable implant for the focal repair of chondral defects in eight adult horses with 12-month follow-up. The bi-layered construct composed of a polycarbonate-urethane-urea biomaterial which was printed in 3D fashion onto a bone anchor was implanted into surgically created osteochondral defects into the femoropatellar joints of eight horses. The analysis of post-mortem outcomes were compared to defects treated with microfracture in the same animal on the contralateral femoropatellar jointfemoropatellar joint. The overall macroscopic scoring after 12 months yielded higher scores in the OCI-treated stifles compared to MF treatment (p = 0.09) with better quality and filling of the defect. Histology revealed good anchorage of repair tissue growing into the 3D structure of the implant and histopathology scoring for adjacent native cartilage showed no difference between groups. MRI and micro-CT showed overall less sclerotic reactions in the surrounding bone in the implant group and no foreign body reaction was detected. Biomechanical analysis of the repair tissue revealed a significantly higher peak modulus (p < 0.05) in the implant group (0.74 ± 0.45) compared to the microfracture control group (0.15 ± 0.11). Dynamic loading yielded higher values for the repair tissue overgrowing the implant group (0.23 ± 0.17) compared to the microfracture control (0.06 ± 0.06) (p < 0.05). The bi-layered osteochondral implant provided a safe implant for focal repair of full-thickness osteochondral defects, as no adverse reaction was seen within the joints and the level of degeneration of adjacent cartilage to the repair site was not different compared to that seen in defects treated with microfracture after 12 months.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 1","pages":"59-69"},"PeriodicalIF":2.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25972","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142255504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0