Journal of Orthopaedic Research®最新文献

{"title":"Assessing the Potential of Antimicrobial Blue Light (aBL) to Manage Biofilm Burden at the Skin-Implant Interface of Percutaneous Osseointegrated Implants.","authors":"Jemi Ong Watts, Richard Tyler Epperson, Brooke Kawaguchi, Aaron Olsen, Josh Tam, Paul Pasquina, Brad Isaacson, Jeremy Gililland, Dustin Williams","doi":"10.1002/jor.70039","DOIUrl":"https://doi.org/10.1002/jor.70039","url":null,"abstract":"<p><p>Percutaneous osseointegrated implants offer significant benefits to amputees, including improved mechanical feedback and reduced skin irritation. However, their percutaneous nature increases susceptibility to biofilm-associated infections. This study evaluated the potential efficacy of antimicrobial blue light (aBL) as a preventive strategy to manage biofilm burden in a 12-week ovine model wherein two treatment cycles were implemented. In this study, aBL was applied proactively to reduce surface colonization and prevent infection progression, rather than to treat confirmed or deep infections. Sheep were inoculated with Staphylococcus aureus biofilm and treated with aBL or systemic antibiotics, with or without a washing protocol. aBL with washing achieved the greatest bacterial reduction, lowering CFU levels to 3.0 ± 0.3 log₁₀ during the second treatment period, significantly outperforming washing alone, which resulted in 5.6 ± 0.4 log₁₀ (p < 0.05). The study also showed a lower observed infection rate (14% vs. 43%), when comparing aBL with washing to washing only based on histologic, microCT, and gross assessments, though no statistical comparison was performed. Despite these benefits, the aBL treatments induced notable tissue changes, including increased epithelial thickening, rete ridge elongation, and accelerated skin/tissue downgrowth. Systemic antibiotics were effective during the initial treatment period but failed to reduce biofilm burden during the second treatment cycle. Altogether, while aBL combined with washing shows promise as a localized preventive therapy for reducing biofilm burden and implant-related infection, the observed tissue remodeling and accelerated downgrowth highlight the need for further investigation to ensure long-term safety.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exercise-Induced Changes in Knee Cartilage In Vivo: Comparing MRI Sequences.","authors":"Natasha K Ivanochko, Anthony A Gatti, Emily G Wiebenga, Michael D Noseworthy, Feliks Kogan, Monica R Maly","doi":"10.1002/jor.70043","DOIUrl":"https://doi.org/10.1002/jor.70043","url":null,"abstract":"<p><p>The purpose was to assess the agreement in measures of acute knee cartilage thickness and composition change after loading in clinical knee osteoarthritis (OA) between two magnetic resonance imaging (MRI) acquisition approaches: (1) single sequence approach using quantitative double-echo in steady-state (qDESS), which allows simultaneous morphological and compositional scanning, versus (2) multi-sequence approach that captures morphology (fast spoiled gradient recalled (FSPGR) or qDESS) and composition (multi-echo spin echo (MESE)) separately. Twenty adults with clinical knee OA participated. 3T MR scans were acquired before and immediately after a 25-min treadmill walk at a standardized speed. Changes in knee cartilage thickness and T2 were assessed. Pre-activity, strong agreement was observed in cartilage thickness captured with qDESS and FSPGR (concordant correlation coefficients 0.842-0.935). Pre-activity, we observed greater absolute cartilage thickness with qDESS compared to FSPGR in femoral cartilage. From pre- to post-activity, qDESS showed change in cartilage thickness in the medial femur (-0.088 ± 0.11 mm, p = 0.002), lateral tibia (-0.042 ± 0.65 mm, p = 0.011) and trochlea (-0.027 ± 0.05 mm, p = 0.024); whereas FSPGR showed a change only in the lateral tibia (-0.064 ± 0.08 mm, p = 0.002). From pre- to post-activity, qDESS showed reduced T2 in all cartilage regions; whereas qDESS + MESE and FSPGR + MESE detected T2 changes in the patella (-1.90 ± 3.00 ms, p = 0.013, and -1.80 ± 2.18 ms, p = 0.002, respectively). qDESS detects transient changes in knee cartilage due to loading in clinical knee OA.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Establishment and Characterization of a Recurrent Osteosarcoma Cell Line: OSA 1777","authors":"","doi":"10.1002/jor.70025","DOIUrl":"10.1002/jor.70025","url":null,"abstract":"<p><b>RETRACTION</b>: P. Thanindratarn, X. Li, D. C. Dean, S. D. Nelson, F. J. Hornicek, and Z. Duan “Establishment and Characterization of a Recurrent Osteosarcoma Cell Line: OSA 1777,” <i>Journal of Orthopaedic Research</i> 38, no. 4 (2020): 902–901, https://doi.org/10.1002/jor.24528.</p><p>The above article, published online on 18 November 2019 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors; the journal Editor-in-Chief, Edward Schwarz; The Orthopaedic Research Society; and John Wiley &amp; Sons, Inc. US.</p><p>The authors requested their article be retracted after they learned that the cell line, OSA 1777, had become contaminated with synovial sarcoma cell line SYO-1. It is unknown at what stage the cell line was contaminated, but as a result, the reported results and conclusions are no longer reliable. Therefore, the article must be retracted.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.70025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of miRNA Biomarkers Associated With Staphylococcal Musculoskeletal Infections.","authors":"Claudia Siverino, Eric Sumrall, Jorge Úbeda Garrido, Jan Puetzler, Andrej Trampuz, Svetlana Karbysheva, Lei Wang, Robert Geoff Richards, Thomas Fintan Moriarty, Elena Della Bella","doi":"10.1002/jor.70042","DOIUrl":"https://doi.org/10.1002/jor.70042","url":null,"abstract":"<p><p>Musculoskeletal infections (MSKIs) pose significant challenges in orthopedic surgery. These infections are often caused by Staphylococcus aureus and S. epidermidis. Early diagnosis is crucial, as it allows prompt treatment before the infection becomes chronic and more difficult to manage. This study evaluated the expression of microRNAs (miRNAs) in the serum of patients with staphylococcal MSKI and their potential as biomarkers. Serum samples from patients with confirmed staphylococcal MSKI were used for RNA sequencing and validation (n = 24 S. aureus, n = 25 S. epidermidis and n = 25 controls). In S. aureus-infected patients compared to uninfected controls, 23 miRNAs were upregulated and 20 downregulated, while 94 miRNAs were upregulated and 31 downregulated in those infected with S. epidermidis. Validation experiments revealed the upregulation of hsa-miR-1246, hsa-miR-483-5p, and hsa-miR-1290, and the downregulation of hsa-miR-1-3p, hsa-miR-148b-3p, and hsa-miR-23a-3p in S. aureus-infected patients compared to both S. epidermidis-infected patients and uninfected controls. Human peripheral blood mononuclear cells treated in vitro with bacterial culture supernatants exhibited a similar pattern of miRNA expression. Receiver operating characteristic (ROC) curve analyses demonstrated that combining four miRNAs (miR-1246, miR-1290, miR-148b-3p, and miR-23a-3p) achieved a high diagnostic accuracy, with an area under the curve of 0.96, distinguishing S. aureus from S. epidermidis-infected patients. In summary, this study identified four differentially expressed miRNAs in S. aureus-infected patients compared to those with S. epidermidis infections and uninfected controls. These findings allow miRNA to be used as MSKI markers and to study their effect on immune responses during bacterial infections.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COX-2 in Fracture Callus Chondro-Osseous Junction Osteoclasts Regulates Chondrocyte Hypertrophy and Callus Vasculogenesis.","authors":"Marc Teitelbaum, Hsuan-Ni Lin, Maya D Culbertson, Charlene Wetterstrand, J Patrick O'Connor","doi":"10.1002/jor.70040","DOIUrl":"https://doi.org/10.1002/jor.70040","url":null,"abstract":"<p><p>Cyclooxygenase-2 (COX-2) activity is necessary for bone fracture healing to proceed normally. COX-2 is encoded by Ptgs2 and is expressed by several cell types during fracture healing, suggesting that COX-2 regulates multiple processes to affect fracture healing. Here, the role of COX-2 expression in osteoclasts during mouse femur fracture healing was examined. Mice lacking COX-2 (Ptgs2-cKO^Lyz2) in osteoclasts and other myeloid cells were made using a floxed COX-2 gene (Ptgs2^tm1Hahe) and cre recombinase expressed from the Lyz2^tm1(cre)If° allele. Fracture healing was assessed by radiology, histology, immunohistochemistry, and mRNA quantification. Targeted loss of COX-2 in osteoclasts was confirmed by immunohistochemistry and led to significant reductions in callus osteoclasts. Comparisons between Ptgs2-cKO^Lyz2 and control mice found significant reductions in callus chondrogenesis and bone formation in the Ptgs2-cKO^Lyz2 mice. The reductions were accompanied by delayed callus vascularization and reduced MMP-13 expression. Immunohistochemistry showed that osteoclasts along the callus chondro-osseous junction normally express COX-2. In Ptgs2-cKO^Lyz2 mice, COX-2 expression was reduced in osteoclasts at the chondro-osseous junction and coincided with reduced MMP-13 expression at the chondro-osseous junction. The results indicate that COX-2 expressed by osteoclasts along the chondro-osseous junction promotes vasculogenesis and regulates chondrocyte hypertrophy during endochondral ossification. The results also indicate that osteoclasts at the callus chondro-osseous junction coordinate multiple cellular processes to promote endochondral ossification.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Patient Outcomes Following Osteochondral Allograft Transplantation: The Impact of 25 Years of Translational and Clinical Research","authors":"Brian J. Cole,&nbsp;Jorge A. Chahla,&nbsp;Susan G. Chubinskaya,&nbsp;Ron Gilat,&nbsp;Rachel M. Frank,&nbsp;Adam B. Yanke","doi":"10.1002/jor.26089","DOIUrl":"10.1002/jor.26089","url":null,"abstract":"<div>\u0000 \u0000 <p>This manuscript consolidates 25 years of interdisciplinary research and clinical advancement in optimizing patient outcomes following osteochondral allograft (OCA) transplantation. In this manuscript, we detail the results of over 1500 OCA procedures and 115 research publications, integrating hypothesis-driven basic science and translational research with clinical outcomes data. The study highlights groundbreaking advancements, including: Innovations in fresh OCA preservation techniques that extend graft viability; Minimization of immunogenic, thermal, and impaction effects to enhance graft integration and durability; Cutting-edge methods for donor-recipient topographic matching, supported by 3D modeling; Evolution of the surgical technique, including the development of orthobiologic approaches to optimize outcomes; Evaluating long-term clinical outcomes and the effect of concomitant procedures; Decision-support algorithms that improve patient selection and surgical planning using machine learning tools. This manuscript illustrates the evolution of OCA transplantation into a reproducible, globally adopted technique for cartilage restoration. By merging basic science, translational insights, and clinical expertise, we redefine and improve the standards of graft handling, surgical technique, and clinical outcomes. The resulting data-driven guidelines and decision-support tools set a foundation for advancing the field, improving accessibility and patient selection, and enhancing patient outcomes worldwide.</p></div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 10","pages":"1705-1723"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D Morphometric Analysis of Femoral Isthmus Dimensions and Their Influence on Modular Kinked Revision Stem Design in Total Hip Arthroplasty.","authors":"Marco Zucchet, Cristiano Pizzamiglio, Beat Schmutz, Chong Bum Chang, Jung-Wee Park, Michele Pressacco","doi":"10.1002/jor.70038","DOIUrl":"https://doi.org/10.1002/jor.70038","url":null,"abstract":"<p><p>The development of stems for femoral revision surgeries requires an understanding of how distal fixation can be achieved, especially in those cases where the curvature of the diaphysis is not negligible, and a straight stem could result in three-point fixation. To this extent, a series of parameters linked to the femoral canal isthmus were identified and their correlation, as well as the potential difference between White and Asian ethnicities, were investigated. The anatomical features of 215 three-dimensional femur models were automatically computed. Statistical inference was performed leveraging the frequentist and Bayesian frameworks. A moderate positive correlation was found between the three-dimensional proximal-isthmus axis angle α and the distance between the isthmus cross-section and proximal-isthmus axis tilting point D_TI (p < 0.001, B₁₀ = 1.1 · 10⁵, ρ = 0.35). White subjects had both significantly longer distance between the head center and isthmus cross-section D_HI (p < 0.001, B₁₀ = 626.57, ∆µ = 10.18 mm) and longer distance between the head center and tilting point D_HT (p < 0.001, B₁₀ = 1.2 · 10⁵, ∆µ = 12.33 mm) with respect to Asian ones. Among all parameters, the correlation between α and D_TI is the most relevant and should be considered in designing a kinked revision stem to determine the best trade-off between fixation area and mechanical stability. The difference between White and Asian patients found for D_HI and the correlation between α and D_HI might have no practical effect in case of modular stem design. The remaining parameters had neither statistically significant correlation/difference nor practical impact on the design. These findings allow to reduce the design variables, thus leading to a more effective design process.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraoperative Navigation, 3D Printed Prostheses, and Conventional Techniques in Femoral and Tibial Tumor Resections: A Systematic Review.","authors":"Nora A Galoustian, Brandon Gettleman, Aura Elias, Mikayla Mefford, Charlotte Wahle, Alexander Christ, Nicholas Bernthal, Lauren E Wessel","doi":"10.1002/jor.70033","DOIUrl":"https://doi.org/10.1002/jor.70033","url":null,"abstract":"<p><p>Technological advancements in orthopaedic oncology continue to augment our approach to surgical interventions. However, the impact on patient outcomes following lower extremity bone tumor resections remains under-characterized. This systematic review identifies the impact of various technological advancements on post-operative outcomes. A systematic search of PubMed, Embase, and Cochrane Library databases (2014-2024) identified studies on lower extremity bone tumor resections involving 3D prostheses/patient-specific instruments (3D PSI), intraoperative navigation, and control conventional surgeries. Individual case reports, cadaveric, and animal studies were excluded. Two reviewers independently screened abstracts and extracted Musculoskeletal Tumor Society (MSTS) scores and data on complications (infections, fractures, revisions). Interventions were segmented by use of technology in surgery between 3D patient-specific instrumentation (PSI), intraoperative navigation and conventional approaches. Descriptive analysis was employed to calculate the weighted means, frequencies, and proportions of complications across technology subgroups. Out of 2841 studies screened, 40 studies with 606 patients met inclusion criteria. Average patient age was 30.7 ± 14.3 years with mean follow-up period of 32.9 ± 20.5 months. Overall complication rates were 11.9 ± 12.9 for 3D PSI, 16.4 ± 16.7% for intraoperative navigation, and 32.7 ± 2.8% for conventional. Weighted mean infection rates were 3.3 ± 6.2% for 3D PSI, 3.4 ± 4.3% for intraoperative navigation, and 7.2 ± 7.8% for conventional. Mean revision rates were 1.6 ± 5.4% for 3D PSI, 2.5 ± 5.6% for intraoperative navigation, and 7.2 ± 3.1% for conventional. Weighted mean MSTS scores were similar between groups (26.4 ± 4.4 3D PSI, 25.4 ± 2.8 intraoperative navigation, 26.2 ± 6.2 conventional). This Level III Evidence study's results suggest that application of technological advances to operative management of musculoskeletal tumors may decrease complication rates while maintaining MSTS scores.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Evaluation of Hemiarthroplasty Bearing Materials: A Scoping Review (Part I).","authors":"Victoria P Marino, Francesca De Vecchi, Takayuki Koya, Landon M Begin, Douglas C Moore, Joseph J Crisco, Markus A Wimmer","doi":"10.1002/jor.70026","DOIUrl":"https://doi.org/10.1002/jor.70026","url":null,"abstract":"<p><p>Hemiarthroplasty is used to treat joint damage confined to one side, aiming to preserve the remaining healthy cartilage and bone while addressing conditions like femur fractures and osteonecrosis; however, challenges persist due to cartilage wear and inconsistent clinical outcomes. This scoping review evaluates in vitro studies on hemiarthroplasty bearing materials (HBM), focusing on the HBM tested, cartilage specimens, experimental setups, outcome variables, and the comparability of results. Of 1798 identified studies, 72 met the inclusion criteria, with 48 classified as in vitro. HBMs tested included metals (42%) and soft polymers (30%) and 45% of studies utilized multiple distinct HBMs. Cartilage specimens were predominantly bovine (65%), mostly from the tibial-femoral joint (69%). The two most common experimental set ups were tribometers (75%) and simulators (25%) both using commercial and custom-built rigs. Tribometer configurations included pin-on-disc and pin-on-plate models and simulators highlighted ball-on-joint set ups. Tribometer motions included linear reciprocating (62%) or multidirectional reciprocating (24%). Input parameters included contact stress (8.8 kPa to 23.3 MPa, mean 2.1 MPa), velocity (0.5-100 mm/s, mean 16.46 mm/s), testing duration (0.03-33.8 h, mean 5.4 h), and distance (5.4 cm to 9000 m, mean 393 m). Simulators used inputs of loads ranging from 25 N to 1.5 kN, 500 to 500,000 cycles and a variety of flexion-extension profiles together with secondary motions. Lubricants were mostly bovine calf serum (38%) and saline-based solutions (21%). Outcome variables mainly included coefficient of friction and damage to cartilage and/or HBM, but the methods to assess these outputs varied. Methodological differences and inconsistent reporting of outcomes limited the ability to draw firm conclusions about the relative performance of HBMs.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Retinoid Antagonist Attenuates Bone Growth Inhibition Caused by Growth Plate Injury in Mice","authors":"Masatake Matsuoka,&nbsp;Ningfeng Tang,&nbsp;Tian Hongying,&nbsp;Kenta Uchibe,&nbsp;Satoru Otsuru,&nbsp;Norimasa Iwasaki,&nbsp;Joshua M. Abzug,&nbsp;Motomi Enomoto-Iwamoto,&nbsp;Masahiro Iwamoto","doi":"10.1002/jor.70032","DOIUrl":"10.1002/jor.70032","url":null,"abstract":"<p>Growth plate injury, which may trigger growth plate fusion or arrest, is a common occurrence in pediatric orthopaedics that can lead to angulation of the limb and limb length discrepancy. Growth plate injuries are currently treated palliatively, with surgical intervention when severe limb length discrepancy or angulation is found during follow-up visits. The cellular changes in the chondrocytes in injured growth plate have not been fully elucidated, and understanding these factors will provide important insights for the development of therapies that promote growth plate repair and prevent growth arrest. In this study, we refined a mouse growth plate injury model and demonstrated that needle-induced growth plate injury resulted in different healing outcomes depending on the size of the needle used. The 30G needle-induced injury was repaired without significant sequelae, while 25G injury induced bone bridge and limb length discrepancy. In the non-healing model, the growth plate showed severely reduced bone-forming activity rapidly after the injury, with a decreased number of osteoclasts in the chondro-osseous junction in the metaphysis. We found that the injured growth plate stimulated retinoid signaling evidenced by the upregulation of retinoid target molecules, and that treatment with 7C, a retinoic acid receptor gamma antagonist, ameliorated decreases in bone forming activity and osteoclast formation, and partially rescued injury-induced growth inhibition. The results of this study suggest that changes in the growth plate immediately post-injury should be considered as a therapeutic target for growth plate repair and that retinoid antagonists may be viable pharmacological interventions for this purpose.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 10","pages":"1724-1735"},"PeriodicalIF":2.3,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.70032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}