Journal of Orthopaedic Research®最新文献_第10页

Evaluating pelvic tilt using the pelvic antero-posterior radiographs: A novel method 利用骨盆前后位X光片评估骨盆倾斜：一种新方法

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-05-30 DOI: 10.1002/jor.25907

Yuan Chai, A. Mounir Boudali, Ewout S. Veltman, John E. Farey, William L. Walter

{"title":"Evaluating pelvic tilt using the pelvic antero-posterior radiographs: A novel method","authors":"Yuan Chai, A. Mounir Boudali, Ewout S. Veltman, John E. Farey, William L. Walter","doi":"10.1002/jor.25907","DOIUrl":"10.1002/jor.25907","url":null,"abstract":"Pelvic tilt (PT) is an important parameter for orthopedic surgeries involving hip and spine, typically determined from sagittal pelvic radiographs. However, various challenges can compromise the feasibility of measurement from sagittal imaging, including obscured landmarks, anatomical variations, hardware interference, and limited medical resources. Addressing these challenges and with the aim of reducing radiation exposure to patients, our study developed a novel method to estimate PT from antero-posterior (AP) radiographs, using vertical distances from the pelvic outlet and obturator foramen. We correlated these measurements with PT, defined both anatomically (anterior pelvic plane, PTa) and mechanically (centers of femoral heads and sacral plate, PTm). The study explored creating linear, exponential, and multivariate regression models based on twelve 3D CT-derived pelvic models (six men, six women), simulating AP radiograph projections with controlled PTs. We then validated these models against 105 pairs of patient stereoradiographs. Statistical analysis revealed that combined exponential-linear models yielded the most accurate results, with Pearson correlation coefficients of 0.75 for PTa and 0.77 for PTm, and mean absolute errors of 3.7° ± 2.6° for PTa and 4.5° ± 3.4° for PTm, showing excellent measurement reliability (all ICCs > 0.9) without significant gender discrepancies. In conclusion, this study presents a validated, simple, and accessible method for estimating PT using AP radiograph parameters, supported by the Supporting Information S1: Excel Tool, showing great potential for clinical application in hip and spine procedures.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25907","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential for hyperbaric oxygen therapy in ACL reconstruction 高压氧疗法在前交叉韧带重建中的潜力。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-05-30 DOI: 10.1002/jor.25911

Nicolas Nadeau, Sean Richards

引用次数: 0

Suppression of TNF-α activity by immobilization rescues Mkx expression and attenuates tendon ossification in a mouse Achilles tenotomy model 在小鼠跟腱切口模型中，通过固定抑制 TNF-α 的活性可拯救 Mkx 的表达并减轻肌腱骨化。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-05-28 DOI: 10.1002/jor.25906

Masashi Isaji, Keisuke Horiuchi, Shinya Kondo, Takahiro Nakagawa, Takahiro Ishizaka, Masatoshi Amako, Kazuhiro Chiba

{"title":"Suppression of TNF-α activity by immobilization rescues Mkx expression and attenuates tendon ossification in a mouse Achilles tenotomy model","authors":"Masashi Isaji, Keisuke Horiuchi, Shinya Kondo, Takahiro Nakagawa, Takahiro Ishizaka, Masatoshi Amako, Kazuhiro Chiba","doi":"10.1002/jor.25906","DOIUrl":"10.1002/jor.25906","url":null,"abstract":"Traumatic heterotopic ossification is a condition in which extraskeletal bone formation occurs in soft tissues after injury. It most commonly occurs in patients who had major orthopedic surgery and in those with severe extremity injuries. The lesion causes local pain and can impair motor function of the affected limb, but there is currently no established prophylaxis or treatment for this condition. In this study, we show that immobilization at an early stage of the inflammatory response after injury can attenuate ossification formation in a murine Achilles tenotomy model. Gene expression analysis revealed a decrease in the expression of Tnf and an increase in the expression of Mkx, which encodes one of the master regulators of tendon differentiation, Mohawk. Notably, we found that TNF-α suppressed the expression of Mkx transcripts and accelerated the osteogenic differentiation of tendon-derived mesenchymal stem cells (MSCs), suggesting that TNF-α acts as a negative regulator of Mkx transcription. Consistent with these findings, pharmaceutical inhibition of TNF-α increased the expression of Mkx transcripts and suppressed bone formation in this mouse model. These findings reveal the previously unrecognized involvement of TNF-α in regulating tendon MSC fate through suppression of Mkx expression and suggest that TNF-α is a potential target for preventing traumatic heterotopic ossification.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relative contribution of the nail and plate to a nail-plate construct for comminuted distal femoral fractures 在治疗股骨远端粉碎性骨折的钉板结构中，钢钉和钢板的作用相对较大。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-05-28 DOI: 10.1002/jor.25908

Charles C. Lin, Nicholas Parody, Utkarsh Anil, Kenneth A. Egol

{"title":"Relative contribution of the nail and plate to a nail-plate construct for comminuted distal femoral fractures","authors":"Charles C. Lin, Nicholas Parody, Utkarsh Anil, Kenneth A. Egol","doi":"10.1002/jor.25908","DOIUrl":"10.1002/jor.25908","url":null,"abstract":"The purpose of this study was to assess the biomechanical contributions of the nail and the plate individually to a complete nail-plate construct in the setting of comminuted distal femur fractures. For this biomechanical study, comminuted extra-articular distal femur fractures were created in 24 synthetic osteoporotic femur models. These were then split into three groups: the nail-only group, the plate-only group, and the nail-plate group. After fixation, each specimen underwent sequential axial and torsional loading, and axial and torsional stiffness were calculated and compared. The addition of a nail to a plate-only construct increased axial stiffness by 19.7% and torsional stiffness by 59.4%. The plate-only group and nail-plate group both demonstrated significantly greater axial and torsional stiffness than the nail-only group at all levels of axial and torsional load. (p < 0.001) At 1000 and 2000 N of cyclic loading, the nail-plate group demonstrated significantly greater axial stiffness than the plate-only group (p ≤ 0.018). The nail-plate group demonstrated greater torsional stiffness than the plate-only groups at all levels of torsional loading (p < 0.001). In osteoporotic comminuted distal femur fracture models, most of the axial stiffness in a nail-plate construct comes from the plate. While the combination of the two constructs is not fully additive, the plate contributes the majority of the axial and torsional stiffness in a nail-plate construct. The supplementation of the plate with a nail primarily helps to increase resistance to rotational forces. Level of Evidence: III.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141157484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-dependent decline of B3AR agonist-mediated activation of FAP UCP-1 expression in murine models of chronic rotator cuff repair 在慢性肩袖修复的小鼠模型中，B3AR 激动剂介导的 FAP UCP-1 表达激活随年龄增长而下降。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-05-26 DOI: 10.1002/jor.25905

Agustin Diaz, Luke Sang, Steven Garcia, Aboubacar Wague, Michael Davies, Alex Youn, Xuhui Liu, Brian T. Feeley

{"title":"Age-dependent decline of B3AR agonist-mediated activation of FAP UCP-1 expression in murine models of chronic rotator cuff repair","authors":"Agustin Diaz, Luke Sang, Steven Garcia, Aboubacar Wague, Michael Davies, Alex Youn, Xuhui Liu, Brian T. Feeley","doi":"10.1002/jor.25905","DOIUrl":"10.1002/jor.25905","url":null,"abstract":"Amibegron, a β3-adrenergic receptor (B3AR) agonist, has recently been shown to provide therapeutic effects for chronic rotator cuff (RC) tears by inducing the expression of uncoupling protein 1 (UCP-1), a marker of brown fat, in fibroadipogenic progenitors (FAPs). However, it remains to be seen if these beneficial effects hold true with age and in older, more clinically relevant populations. This study seeks to understand the impacts of aging on the efficacy of amibegron to treat chronic RC tears. Young (4-month-old) and aged (33-month-old) C57BL/6 mice underwent a RC injury procedure with delayed repair (DR). Mice were equally randomized to receive amibegron or dimethyl sulfoxide (DMSO) treatments after repair. Functional ability was measured at baseline and 6-weeks after DR. Wet muscle weight and histology of injured and contralateral supraspinatus were also analyzed 6-weeks post-DR. For in vitro histology and real-time quantitative PCR experiments, FAPs were isolated from young and aged mice via fluorescence-activated cell sorting. Young and aged FAPs were treated with amibegron or DMSO either immediately after seeding (early exposure) or 8-days after seeding (late exposure). In vitro results showed that amibegron-mediated FAP UCP-1 expression decreases with age. In vivo data demonstrated that aged mice have a decreased responsiveness to amibegron and decreased propensity for intramuscular FAP UCP-1 expression. Further, delayed amibegron treatment with RC repair did not lead to improvements in muscle atrophy and functional outcomes. Our findings demonstrate that age and the timing of interventions play a critical role in FAP-targeted therapeutics for chronic injuries.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25905","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Duration of cefazolin prophylaxis did not impact infection risk in a murine model of joint arthroplasty 头孢唑啉预防性治疗的持续时间不会影响小鼠关节置换术模型的感染风险。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-05-26 DOI: 10.1002/jor.25903

Melissa J. Karau, Diego Alarcon Perico, Serigo F. Guarin Perez, Christina Koscianski, Matthew P. Abdel, Robin Patel, Nicholas A. Bedard

{"title":"Duration of cefazolin prophylaxis did not impact infection risk in a murine model of joint arthroplasty","authors":"Melissa J. Karau, Diego Alarcon Perico, Serigo F. Guarin Perez, Christina Koscianski, Matthew P. Abdel, Robin Patel, Nicholas A. Bedard","doi":"10.1002/jor.25903","DOIUrl":"10.1002/jor.25903","url":null,"abstract":"To minimize periprosthetic joint infection (PJI) risk, some clinicians prescribe extended antibiotic prophylaxis (EAP) following total joint arthroplasty (TJA). Given the limited evidence supporting EAP, we sought to evaluate impact of prophylactic antibiotic duration on PJI risk in a murine TJA model. A titanium prosthesis was implanted into the proximal tibia of 89 mice and inoculated with 102 colony forming units (cfu) of Staphylococcus aureus Xen36. Control mice (n = 20) did not receive antibiotics. Treated mice received either 24 h (n = 35) or 4 days (n = 34) of cefazolin prophylaxis. Cultures were obtained from the prostheses, tibia, femur, and knee tissues 3 weeks after surgery. All mice in the control group developed PJI. Both prophylaxis regimens reduced the rate of PJI relative to the control, with only 2/35 mice in the 24-h cohort (p < 0.0001) and 1/34 in 4-day cohort developing PJI (p < 0.0001). CFU counts from the prostheses, bone and knee tissues were reduced for the 24-h and 4-day prophylaxis cohorts relative to the control (p < 0.0001 for both). There was no difference in rates of PJI or CFU counts between the two prophylaxis cohorts (p = 0.58). Prophylactic cefazolin profoundly reduced rates of PJI in a murine model of TJA in which all control animals developed PJI. Extending cefazolin prophylaxis duration from 24 h to 4 days did not result in improved PJI rates or decreased bacterial loads in infected cases. While these results strongly support use of antibiotic prophylaxis for TJA, EAP did not appear to add benefit in the described mouse model.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of early metaphyseal changes in a piglet model of Legg-Calvé-Perthes disease using quantitative mapping of MRI relaxation times 利用磁共振成像弛豫时间定量绘图检测 Legg-Calvé-Perthes 病小猪模型中的早期骺变化。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-05-26 DOI: 10.1002/jor.25904

Erick O. Buko, Alexandra R. Armstrong, Jennifer C. Laine, Ferenc Tóth, Casey P. Johnson

{"title":"Detection of early metaphyseal changes in a piglet model of Legg-Calvé-Perthes disease using quantitative mapping of MRI relaxation times","authors":"Erick O. Buko, Alexandra R. Armstrong, Jennifer C. Laine, Ferenc Tóth, Casey P. Johnson","doi":"10.1002/jor.25904","DOIUrl":"10.1002/jor.25904","url":null,"abstract":"Legg-Calvé-Perthes disease (LCPD) is a childhood hip disorder characterized by ischemic injury to the epiphysis of the femoral head, but changes to the metaphysis have also been implicated in its pathogenesis. Quantitative magnetic resonance imaging (MRI) relaxation time mapping techniques are potentially useful to detect injury in LCPD, but studies to date have focused on the epiphysis. The purpose of this study was to assess whether T2, T1ρ, adiabatic T1ρ, and adiabatic T2ρ relaxation times can detect early metaphyseal changes in an LCPD piglet model. Complete epiphyseal ischemia of one femoral head was surgically induced and confirmed using contrast-enhanced MRI in n = 10 6-week-old piglets; the contralateral side was unoperated. The bilateral hips were imaged 1 week after surgery in vivo at 3T MRI using relaxation time mapping and contrast-enhanced MRI. Relaxation times and thicknesses of the metaphyseal primary and secondary spongiosa were measured and compared between the ischemic and contralateral-control femoral heads using paired t-tests. In the ischemic femoral heads, T2 relaxation times were significantly increased in the primary spongiosa (6.7 ± 9.8 ms, p = 0.029), and T2, T1ρ, adiabatic T1ρ, and adiabatic T2ρ relaxation times were significantly decreased in the secondary spongiosa (respectively: −13.3 ± 9.3 ms, p = 0.013; −32 ± 23 ms, p < 0.001; −43 ± 41 ms, p = 0.009; and −39 ± 13 ms, p < 0.001). The secondary spongiosa thickness was also significantly decreased in the ischemic femoral heads (p < 0.001). In conclusion, T2, T1ρ, adiabatic T1ρ, and adiabatic T2ρ relaxation time mapping techniques can detect early changes in the metaphysis following ischemic injury to the epiphysis of the femoral head in a piglet model of LCPD.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25904","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of amniotic suspension allograft in a rat destabilization of medial meniscus osteoarthritis model 羊膜悬液异体移植在大鼠内侧半月板骨关节炎不稳定模型中的效果。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-05-23 DOI: 10.1002/jor.25872

Katrina A. Harmon, Kelly A. Kimmerling, Katie C. Mowry

{"title":"Effect of amniotic suspension allograft in a rat destabilization of medial meniscus osteoarthritis model","authors":"Katrina A. Harmon, Kelly A. Kimmerling, Katie C. Mowry","doi":"10.1002/jor.25872","DOIUrl":"10.1002/jor.25872","url":null,"abstract":"Placental-derived allografts have been of interest as a potential nonsurgical treatment to reduce pain and improve function in knee osteoarthritis (OA). The purpose of this study was to evaluate the effect of single and repeat injection of amniotic suspension allograft (ASA) on pain, function, and cytokine levels using a destabilization of the medial meniscus (DMM) rat model of OA. Post-DMM surgery, animals were treated with a single injection of either ASA, vehicle, or triamcinolone, or repeated injection of either ASA or vehicle. Behavioral testing including knee swelling, pain threshold, dynamic weight bearing (DWB), and gait analysis were evaluated during the in-life phase. Postsacrifice, histopathology and serum and synovial fluid analyses were evaluated. Significant improvements in both DWB differentials and pain threshold were seen in response to repeated injection of ASA, while a single injection of ASA and triamcinolone resulted in significant improvements in pain threshold. Histopathology analysis found no significant differences regardless of treatment compared to vehicle, except for an increase in synovitis following repeated injection of ASA. A single injection of ASA and triamcinolone resulted in increased anti-inflammatory cytokines; repeated ASA injection resulted in significant increases in several immune-modulating factors relevant to OA. When comparing the impact of single and repeat ASA treatments on behavioral testing, repeated injection provided significant additional improvements in both pain and function. This study provides evidence demonstrating the impact of a second injection while also providing additional data for evaluating the use of ASA as a nonsurgical treatment for knee OA.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current trends in the prevention of adhesions after zone 2 flexor tendon repair 预防第 2 区屈肌腱修复术后粘连的当前趋势。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-05-18 DOI: 10.1002/jor.25874

Chaiyos Vinitpairot, Jasper H. N. Yik, Dominik R. Haudenschild, Robert M. Szabo, Christopher O. Bayne

{"title":"Current trends in the prevention of adhesions after zone 2 flexor tendon repair","authors":"Chaiyos Vinitpairot, Jasper H. N. Yik, Dominik R. Haudenschild, Robert M. Szabo, Christopher O. Bayne","doi":"10.1002/jor.25874","DOIUrl":"10.1002/jor.25874","url":null,"abstract":"Treating flexor tendon injuries within the digital flexor sheath (commonly referred to as palmar hand zone 2) presents both technical and logistical challenges. Success hinges on striking a delicate balance between safeguarding the surgical repair for tendon healing and initiating early rehabilitation to mitigate the formation of tendon adhesions. Adhesions between tendon slips and between tendons and the flexor sheath impede tendon movement, leading to postoperative stiffness and functional impairment. While current approaches to flexor tendon repair prioritize maximizing tendon strength for early mobilization and adhesion prevention, factors such as pain, swelling, and patient compliance may impede postoperative rehabilitation efforts. Moreover, premature mobilization could risk repair failure, necessitating additional surgical interventions. Pharmacological agents offer a potential avenue for minimizing inflammation and reducing adhesion formation while still promoting normal tendon healing. Although some systemic and local agents have shown promising results in animal studies, their clinical efficacy remains uncertain. Limitations in these studies include the relevance of chosen animal models to human populations and the adequacy of tools and measurement techniques in accurately assessing the impact of adhesions. This article provides an overview of the clinical challenges associated with flexor tendon injuries, discusses current on- and off-label agents aimed at minimizing adhesion formation, and examines investigational models designed to study adhesion reduction after intra-synovial flexor tendon repair. Understanding the clinical problem and experimental models may serve as a catalyst for future research aimed at addressing intra-synovial tendon adhesions following zone 2 flexor tendon repair.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Target NF-κB p65 for preventing posttraumatic joint contracture in rats 针对 NF-κB p65 预防大鼠创伤后关节挛缩

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-05-15 DOI: 10.1002/jor.25877

Lingpeng Kong, Yuqing Liang, Jing Hou, Weiying Zhang, Shichao Jiang

{"title":"Target NF-κB p65 for preventing posttraumatic joint contracture in rats","authors":"Lingpeng Kong, Yuqing Liang, Jing Hou, Weiying Zhang, Shichao Jiang","doi":"10.1002/jor.25877","DOIUrl":"10.1002/jor.25877","url":null,"abstract":"RelA/p65 is as a crucial component of the nuclear factor κB (NF-κB) signaling pathway that has a significant impact on various fibrotic diseases. However, its role in the fibrosis of tissues surrounding the joint after traumatic injury remains unclear. In this study, rats were divided into three groups: non-operated control (NC) group, p65-siRNA treated (siRNA-p65) group, and negative siRNA treated (siRNA-neg) group. Then, 10 μL (10 nmol) of p65-siRNA was injected into the joint of the siRNA-p65 group. Meanwhile, 10 μL of negative siRNA was administered to the knee joint of the operated siRNA-neg group for comparison. The rats in the NC group did not receive surgery or drug intervention. After 4 weeks of right knee fixation in each group, X-ray measurements revealed significantly reduced degree of knee flexion contracture following p65-siRNA treatment (siRNA-neg: 77.73° ± 2.799°; siRNA-p65: 105.7° ± 2.629°, p < 0.0001). Histopathological examination revealed that the number of dense fibrous connective tissues decreased following p65-siRNA inhibition. Western blot analysis revealed significantly different expression levels of fibrosis-related proteins between the siRNA-p65 and siRNA-neg groups. Immunohistochemical analysis revealed a reduction in the average number of myofibroblasts in the siRNA-p65 group compared with that in the siRNA-neg group. Thus, intra-articular p65-siRNA injection could attenuate fibroblast activation and fibrosis-related protein production, suppress periarticular tissue fibrosis, and prevent joint contracture by downregulating the NF-κB p65 pathway. Statement of clinical significance: Intra-articular injection of p65-siRNA could reduce myofibroblast proliferation and fibrosis-related protein expression by downregulating the NF-κB p65 pathway, inhibit periarticular tissue fibrosis, and prevent joint adhesion, which represents a potential therapy in the prevention of joint fibrosis following traumatic injury.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140945044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0