Journal of Orthopaedic Research®最新文献

{"title":"A High-Riding Patella Is a Feature of Knee Joint Motion During Gait After ACL Reconstruction","authors":"Marcus G. Pandy,&nbsp;Hans A. Gray,&nbsp;Padma N. Ganapam,&nbsp;Adam G. Culvenor,&nbsp;Kay M. Crossley,&nbsp;Shanyuanye Guan","doi":"10.1002/jor.26062","DOIUrl":"10.1002/jor.26062","url":null,"abstract":"<p>Accurate measurements of knee joint motion during gait after anterior cruciate ligament reconstruction (ACLR), especially for the patellofemoral joint, are sparse. The aim of this study was to measure six-degree-of-freedom (6-DOF) patellofemoral and tibiofemoral motion in ACLR and uninjured contralateral knees during gait, and to compare these results to healthy (control) knees. Biplane fluoroscopy was used to measure 6-DOF patellofemoral and tibiofemoral motion in 15 ACLR participants (26.3 ± 3.9 years) for complete cycles of level walking and downhill walking, and the results were compared to data for 10 healthy individuals (29.8 ± 6.1 years). Mean patellar superior translation, anterior translation, and flexion over the gait cycle were respectively 4.4–5.6 mm greater, 5.4–6.3 mm greater, and 3.7°–7.0° less in the ACLR and contralateral knees compared to controls across both activities (<i>p</i> &lt; 0.021). Articular contact was 7.6 mm higher on the femoral trochlea in the ACLR and contralateral knees compared to controls. The patellar tendon was 8.9 mm longer (<i>p</i> &lt; 0.001) in the ACLR and contralateral knees compared to controls. Among ACLR participants, 14 out of 30 knees (47%) had an Insall–Salvati ratio ≥ 1.20, indicating patella alta. Mean tibial external rotation and anterior translation over the gait cycle were respectively 3.4°–3.8° greater and 2.6–3.0 mm greater in the ACLR knee compared to controls across both activities (<i>p</i> &lt; 0.025). A high-riding patella in both knees of the ACLR participants was due to a longer patellar tendon. A change in the load-bearing areas of the femoral trochlea may contribute to the high rate of patellofemoral osteoarthritis seen after ACLR.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 5","pages":"1024-1034"},"PeriodicalIF":2.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.26062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classification of Kinematic Coupling Patterns Between Lower Limb Joints During Running","authors":"Shiori Hiratsuka,&nbsp;Tomoya Takabayashi,&nbsp;Takahiro Watanabe,&nbsp;Takanori Kikumoto,&nbsp;Masayoshi Kubo","doi":"10.1002/jor.26074","DOIUrl":"10.1002/jor.26074","url":null,"abstract":"<div>\u0000 \u0000 <p>Abnormal foot alignment such flatfoot is involved in running injuries. The mechanism by which flatfoot is susceptible to running injuries has not yet been established. The purpose of this study was to classify kinematic coupling patterns by hierarchical clustering, adding individuals with flatfoot and motion in the transverse plane involved in running injuries, and to identify the factors that determine the clusters classified. Twenty-four young healthy adults were divided and classified into normal and flat foot groups of 12 participants each, using the foot posture index. The task involved treadmill running. Cross-correlation coefficients between the ankle and knee joints and between the knee and hip joint were used to evaluate kinematic coupling in the sagittal, frontal, and transverse planes during the stance phase. Hierarchical clustering was used to classify kinematic coupling patterns using the cross-correlation coefficient as an input variable. After cluster classification, joint angles during the stance phase and static standing were compared between clusters. Hierarchical clustering classified kinematic coupling patterns between lower limb joints into two clusters, mainly the kinematic coupling on the frontal plane. However, clusters were not classified by foot alignment. Significant differences in knee varus/valgus, hip adduction/abduction, and internal/external rotation angles during the stance phase were observed between clusters. Foot alignment was not involved in the cluster analysis in this study. Further detailed evaluation of foot alignment is required.</p>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 6","pages":"1171-1178"},"PeriodicalIF":2.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Rotational Guided Growth With Flexible Tethers on the Distal Femoral Bone: An Experimental Study in a Porcine Model","authors":"Ahmed Halloum,&nbsp;Maria Tirta,&nbsp;Søren Kold,&nbsp;Shima Gholinezhad,&nbsp;Jan Duedal Rölfing,&nbsp;John Rasmussen,&nbsp;Ahmed A. Abood,&nbsp;Ole Rahbek","doi":"10.1002/jor.26073","DOIUrl":"10.1002/jor.26073","url":null,"abstract":"<p>New and innovative surgical techniques, using a flexible tether to correct rotational deformities of the growing femur have recently emerged, but are lacking radiographic validation of desired rotation and adverse effects in the frontal and sagittal planes. We, therefore, investigated this new technique in an established large animal model. Eight skeletally immature pigs were included in the study in a paired (left vs. right leg) design. Mean (range) observation time was 83 days (77–90). CT-scans and X-rays were performed immediately after insertion of the tethers and again at euthanasia after 12 weeks. From the CT-scans, 3D-models of the left and right femora were generated and analyzed for intended external rotation and unintended effects in other planes and bone morphology. One pig was excluded due to surgical site infection. Average change in rotation between the operated and non-operated femora was 7.2° (CI: 5.9–8.6) external rotation. We also found unintended valgus deformity in the coronal plane (7.8° (CI: 3.9–12.0) and unintended extension in the sagittal plane (3.9° (CI: −1.8 to 8.7). Unintended changes to the joint morphology were also observed. Rotation by guided growth was achieved with this surgical technique, confirmed by radiological measurements.</p><p><b>Clinical Significance.</b> While this large animal study has shown that this new surgical technique reliably corrected rotational deformities by guided growth, it has also revealed adverse effects that need further investigation before clinical use.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 6","pages":"1164-1170"},"PeriodicalIF":2.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.26073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The LINC Complex Regulates Tendon Elastic Modulus, Collagen Crimp, and Lateral Expansion During Early Postnatal Development","authors":"Nicholas M. Pancheri,&nbsp;Jordan T. Daw,&nbsp;Destinee Ditton,&nbsp;Nathan R. Schiele,&nbsp;Scott Birks,&nbsp;Gunes Uzer,&nbsp;Calvin L. Jones,&nbsp;Brian T. Penney,&nbsp;Sophia K. Theodossiou","doi":"10.1002/jor.26069","DOIUrl":"10.1002/jor.26069","url":null,"abstract":"<div>\u0000 \u0000 <p>There is limited understanding of how mechanical signals regulate tendon development. The nucleus has emerged as a major regulator of cellular mechanosensation via the linker of nucleoskeleton and cytoskeleton (LINC) protein complex. Specific roles of LINC in tenogenesis have not been explored. In this study, we investigate how LINC regulates tendon development by disabling LINC-mediated mechanosensing via dominant negative (dn) overexpression of the Klarsicht, ANC-1, and Syne Homology (KASH) domain, which is necessary for LINC to function. We hypothesized that LINC regulates mechanotransduction in developing tendons and that disabling LINC would impact tendon's mechanical properties and structure in a mouse model of dnKASH. We used Achilles tendon (AT) and tail tendon (TT) as representative energy-storing and positional tendons, respectively. Mechanical testing at postnatal day 10 showed that disabling the LINC complex via dnKASH significantly impacted tendon mechanical properties and cross-sectional area and that the effects differed between ATs and TTs. Collagen crimp distance was also impacted in dnKASH tendons and was significantly decreased in ATs and increased in TTs. Overall, we show that disruption to the LINC complex specifically impacts tendon mechanics and collagen crimp structure, with unique responses between an energy-storing and limb-positioning tendon. This suggests that nuclear mechanotransduction through LINC plays a role in regulating tendon formation during neonatal development.</p>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 6","pages":"1090-1100"},"PeriodicalIF":2.1,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Grip Force Modulation on Median Nerve Morphology Changes","authors":"Shengwei Li,&nbsp;Aaron M. Kociolek,&nbsp;Lizbeth A. Mariano,&nbsp;Ping Yeap Loh","doi":"10.1002/jor.26068","DOIUrl":"10.1002/jor.26068","url":null,"abstract":"<p>Compression on the median nerve can lead to carpal tunnel syndrome (CTS), and median nerve indicators measured from ultrasound images can be used for CTS diagnosis. The aim of this study was to investigate the relationship between grip force modulation and dynamic morphological changes of the median nerve. We used a digital grip dynamometer to measure grip force while simultaneously conducting ultrasound examinations. Ultrasound images were sampled for both the dominant and nondominant hands of all participants (<i>n</i> = 20) during a baseline condition at approximately 0% maximum voluntary force (MVF), during sustained grip force conditions at 25%, 50%, 75%, and 100% MVF, and during the return to a relaxed state (≈0% MVF) directly following each grip force condition. Regardless of hand dominance, grip force level, and grip force modulation, median nerve cross-sectional area (MNCSA) during the grip tasks was smaller relative to the initial baseline condition without grip force. With respect to shape change, the median nerve became more flattened, including increased longitudinal diameter (D1) and decreased vertical diameter (D2), when grip force was relaxed compared to the preceding sustained grip force condition for the dominant hand; however, there were no significant shape changes for the nondominant hand. As morphological changes to tissue result in strain, our results indicate that median nerve injury development may be associated with more hand usage (dominant hand, grip exertion, and grip force modulation), and further suggests the evaluative potential for median nerve dynamics within the carpal tunnel.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 6","pages":"1179-1190"},"PeriodicalIF":2.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.26068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information - Cover","authors":"","doi":"10.1002/jor.25885","DOIUrl":"https://doi.org/10.1002/jor.25885","url":null,"abstract":"","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25885","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information - Editorial Board and TOC","authors":"","doi":"10.1002/jor.25884","DOIUrl":"https://doi.org/10.1002/jor.25884","url":null,"abstract":"","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 4","pages":"715-718"},"PeriodicalIF":2.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25884","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remodeling Process of the Tendon Graft After Anterior Cruciate Ligament Reconstruction: Comprehensive Analysis With RNA Sequencing in a Murine Model","authors":"Susumu Wada,&nbsp;Takayuki Fujii,&nbsp;Camila B. Carballo,&nbsp;Yuki Suzuki,&nbsp;Zhe Song,&nbsp;Yake Liu,&nbsp;Xueying Zhang,&nbsp;Brett J. Croen,&nbsp;Reyna Bhandari,&nbsp;Xiang-Hua Deng,&nbsp;Scott A. Rodeo","doi":"10.1002/jor.26065","DOIUrl":"10.1002/jor.26065","url":null,"abstract":"<div>\u0000 \u0000 <p>The tendon graft is known to undergo a remodeling process after anterior cruciate ligament (ACL) reconstruction. However, little is known about the transcriptional profile of this process. The aim of the present study is to identify differentially expressed genes inside the remodeling ACL graft in the early phase after ACL reconstruction in our murine model using RNA sequencing (RNAseq). Fifty four male C57BL/6 mice were used in this study. The mice were euthanized at 1, 2, and 4 weeks after surgery and used for histological evaluations and RNAseq of the tendon graft. Histologically, there was a progressive decrease in the tendon-bone interface gap space and increased tissue continuity between the grafted tendon and the bone tunnel over time. At 1 and 2 weeks after surgery, cell increase and loss of collagen fiber organization inside the tendon graft were observed. RNAseq showed that genes related to inflammation, matrix metalloproteinases, bone metabolism, chemokines and signaling pathways were upregulated at 1 and 2 weeks after surgery compared to the control group (<i>p</i> &lt; 0.0001). Our transcriptional profiling data suggests that expression of inflammatory mediators and bone remodeling genes may play an important role in the early events in graft-to-bone healing. Further validation at the protein level is necessary to draw firm conclusions about the role of these mediators in graft remodeling and healing. Understanding the remodeling process of the grafted tendons may lead to the identification of new approaches to improve clinical outcomes after ACL reconstruction.</p>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 6","pages":"1122-1131"},"PeriodicalIF":2.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extensive Periosteal Injury During Fracture Induces Long-Term Pain in Mice","authors":"Jiaoni Li,&nbsp;Yu-Ru Vernon Shih,&nbsp;Huchen Tao,&nbsp;Mitchell Negus,&nbsp;Jeffrey Bohrer Guerra,&nbsp;Shyni Varghese","doi":"10.1002/jor.26067","DOIUrl":"10.1002/jor.26067","url":null,"abstract":"<div>\u0000 \u0000 <p>Bone fractures pose a significant public health challenge, often necessitating surgical interventions to facilitate bone healing and functional recovery. Sensory nerve fibers innervate various compartments of the bone tissue, with the periosteum exhibiting the most extensive innervation that is susceptible to injury during trauma. Despite its importance, the effect of injured periosteum on fracture pain remains unknown. This study examines the impact of extensive periosteal injury on fracture pain by using a mouse model. Periosteal injury is induced by mechanical resection during unilateral transverse fracture and compared to transverse fractures with no periosteal injury. Our results demonstrate that extensive periosteal injury induces severe and long-term pain, as assessed by von Frey and dynamic weight bearing measurements, for up to 12 weeks postfracture. Immunofluorescence staining revealed an increase in local neurofilament heavy polypeptide (NF200 +) nerve innervation and an elevated number of calcitonin gene-related peptide (CGRP +) expressing neurons in the dorsal root ganglion (DRG). Additionally, flow cytometric analyses revealed increased presence of myeloid immune cells in the DRG. Furthermore, bone healing in fractures with extensive periosteal injury exhibited reduced callus size at all time points as assessed by Faxitron X-ray imaging. This study describes a previously unknown effect of extensive periosteal injury in exacerbating fracture pain and establishes a potential model to study long-term orthopedic fracture pain.</p>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 6","pages":"1155-1163"},"PeriodicalIF":2.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation Controls the Expression of Lipoxin Receptors, Promoting the Resolution of the Inflammatory Process in an Experimental Tendinitis Model","authors":"Rodrigo Labat Marcos,&nbsp;Mateus Moura Evaristo,&nbsp;Patrícia de Almeida-Mattos,&nbsp;Cristina Nunes Capeloa,&nbsp;José Maria Miguel Aguilera Cantero,&nbsp;Renato Araújo Prates,&nbsp;José Antônio Silva Junior,&nbsp;Andrey Jorge Serra,&nbsp;Leonardo Longo,&nbsp;Nivaldo Antonio Parizotto,&nbsp;Rodrigo Álvaro Brandão Lopes Martins","doi":"10.1002/jor.26063","DOIUrl":"10.1002/jor.26063","url":null,"abstract":"<div>\u0000 \u0000 <p>Tendinopathies are a significant global health issue due to their detrimental effects on mobility and quality of life. Pharmacological treatments, although widely used for pain management, often demonstrate limited efficacy. Photobiomodulation therapy (PBM) has emerged as a potential adjunctive treatment due to its capacity to modulate inflammation and alleviate pain. Nevertheless, further research is required to elucidate its mechanisms of action, particularly concerning the resolution of the inflammatory process. This study aimed to evaluate the effects of PBM on inflammation control in an experimental tendinitis model by analyzing inflammatory infiltrate, myeloperoxidase (MPO) activity, the expression of inflammatory and resolution markers (TNF-α, TGF-β, COX-2, and ALX), and protein levels of PGE2 and COX-2 in rat Achilles tendons with type I collagen-induced tendinitis. Male Wistar rats were randomized into five groups: healthy control (CTL), untreated tendinitis (NT), PBM-treated tendinitis (830 nm; 3 J; 30 mW; 64 J/cm²), or tendinitis treated with sodium diclofenac (DIC; 1 mg/kg IM). After 2 or 12 h, tissues and blood were collected for biochemical and histological analysis. The NT group exhibited increased inflammatory infiltrate, MPO activity (<i>p</i> &lt; 0.001), COX-2, TNF-α (<i>p</i> &lt; 0.001), and PGE2 expression (<i>p</i> &lt; 0.01) but lacked ALX receptor upregulation. PBM and DIC treatments significantly reduced inflammatory infiltrate and MPO activity (PBM: <i>p</i> &lt; 0.001; DIC: <i>p</i> &lt; 0.01). PBM enhanced ALX and TGF-β expression (<i>p</i> &lt; 0.001) and maintenance of COX-2 similar to the NT group, suggesting lipoxin involvement in inflammation resolution. These findings highlight PBM as a promising therapy for tendinopathies by targeting both inflammatory and resolution pathways.</p>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 5","pages":"1035-1044"},"PeriodicalIF":2.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}