Journal of Orthopaedic Research®最新文献

{"title":"Acetabular Bone Preservation in H1 Ceramic Hip Resurfacing: A Comparison With A Conventional Metal-on-Metal Hip Resurfacing","authors":"Yuki Yamamuro,&nbsp;Simon Harris,&nbsp;Rima Nasser,&nbsp;Amir Ardakani,&nbsp;Justin Cobb","doi":"10.1002/jor.70221","DOIUrl":"10.1002/jor.70221","url":null,"abstract":"<p>This study compared the volume of acetabular bone resection between an anatomically designed cementless ceramic-on-ceramic hip resurfacing arthroplasty (H1HR) and metal-on-metal hip resurfacing (Birmingham Hip Resurfacing; BHR). We hypothesized that the anatomical design of the H1 cup would reduce the need for medialization to avoid rim overhang, thereby preserving acetabular bone stock. A retrospective three-dimensional simulation study was performed in 12 hips with osteoarthritis and femoral head size ≥ 48 mm. H1HR and BHR were virtually implanted using 3D planning software to minimize the risk of groin pain. The primary outcome was the volume of acetabular bone resection. Secondary outcomes included the position of the cup relative to the native hip centre and the cup non-coverage ratio. H1 cups required a mean volume of acetabular bone resection of 11 cm³, while BHR cups required 17 cm³. This 35% reduction was statistically significant. H1 cups had less medialization (−1 mm vs. −6 mm), superior displacement (−1 mm vs. −2 mm), and posterior displacement (−1 mm vs. −3 mm). The mean cup non-coverage ratio was not significantly different (10% vs. 8%). The anatomically designed H1HR achieved a significant 35% reduction in acetabular bone resection compared with BHR. This substantial volume of bone preservation was primarily attributed to the design enabling cup placement with minimal medialization, without increasing the cup non-coverage ratio. Although further validation in cadaveric or postoperative imaging studies is needed, H1 may be a promising option for bone preservation, particularly for young, active patients who might require future revision surgery.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"44 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13150472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delayed Progression of Meniscal Degeneration in Silent Information Regulator 2 Ortholog 1 Knock-In Mouse","authors":"Shohei Sano,&nbsp;Ryo Okada,&nbsp;Tetsuya Yamamoto,&nbsp;Kenjiro Okimura,&nbsp;Kiminari Kataoka,&nbsp;Koji Nukuto,&nbsp;Yuta Nakanishi,&nbsp;Masanori Tsubosaka,&nbsp;Kyohei Nishida,&nbsp;Kanto Nagai,&nbsp;Naoki Nakano,&nbsp;Noriyuki Kanzaki,&nbsp;Yuichi Hoshino,&nbsp;Tomoyuki Matsumoto,&nbsp;Ryosuke Kuroda,&nbsp;Takehiko Matsushita","doi":"10.1002/jor.70218","DOIUrl":"10.1002/jor.70218","url":null,"abstract":"<p>This study aimed to examine the inhibitory effects of silent information regulator 2 ortholog 1 (SIRT1) on meniscal degeneration. First, SIRT1 expression in human and mouse menisci was examined using reverse transcription polymerase chain reaction (PCR) and immunostaining, which confirmed its presence. Meniscus degeneration in wild-type (WT) and SIRT1-knock-in (KI) mice was evaluated at 1, 3, 6, 9, and 12 months of age using the Kwok scoring system. The meniscus degeneration score for the anterior segment was significantly lower in KI mice than in WT mice at 9 and 12 months of age (<i>p</i> &lt; 0.01). Furthermore, osteoarthritis (OA) was induced in 3-month-old mice via medial meniscus destabilization to evaluate meniscal degeneration during OA progression. In the OA model, the meniscus degeneration score for anterior segment in KI mice was significantly lower than those in WT mice at 8, 12, and 16 weeks post-surgery (<i>p</i> &lt; 0.01). Additionally, RNA was extracted from the menisci of 1- and 3-month-old KI and WT mice, and gene expression was analyzed using real-time PCR. Gene expression analysis showed significantly higher levels of <i>Sirt1</i>, <i>Col1a1</i>, <i>Col2a1</i>, and <i>aggrecan</i>, and lower levels of <i>Mmp-3</i>, <i>Mmp-13</i>, and <i>Adamts-5</i> in KI mice at 3 months compared with WT mice. These findings suggest that SIRT1 may delay meniscal degeneration by modulating the expression of cartilage matrix genes and matrix-degrading enzymes.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"44 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Radioimmunotherapy and Antibiotics on Biofilm-Associated Implant Infections in a Preclinical Rat Model","authors":"F. Ruben H.A. Nurmohamed,&nbsp;Kevin J. H. Allen,&nbsp;Mackenzie E. Malo,&nbsp;Connor Frank,&nbsp;Colleen Nesbitt,&nbsp;J. Fred F. Hooning van Duyvenbode,&nbsp;Michelle Buijs,&nbsp;Berend van der Wildt,&nbsp;Alex J. Poot,&nbsp;Marnix G. E. H. Lam,&nbsp;Jos A. G. van Strijp,&nbsp;Johannes M. van der Merwe,&nbsp;H. Charles Vogely,&nbsp;Harrie Weinans,&nbsp;Bart C. H. van der Wal,&nbsp;Ekaterina Dadachova","doi":"10.1002/jor.70216","DOIUrl":"10.1002/jor.70216","url":null,"abstract":"<p>Indwelling medical implants are susceptible to developing biofilm-associated infections that are notoriously difficult to eradicate. These persistent infections often cannot be resolved with antibiotics alone and typically require surgical intervention for effective management. An alternative approach is radioimmunotherapy (RIT) which uses specific antibodies linked to radioisotopes to selectively destroy bacteria. This antimicrobial approach bypasses traditional antibiotic mechanisms, and RIT is hypothesized to enhance outcomes beyond antibiotic therapy alone. RIT bactericidal effects were studied in Wistar Han rats fitted with femoral rod implants covered by matured 3-day biofilms. The rats (six per group) were treated with either: RIT with ¹⁷⁷Lu-labeled 4497 antibody to <i>S. aureus</i> teichoic wall acid (WTA) (116.6 MBq/kg), or vancomycin (88 mg/kg), or combination of RIT (116.8 MBq/kg) and vancomycin, or left untreated. To evaluate efficacy, bacterial counts were taken from the joint capsule, bone, and implant after 7 days. Uptake and biodistribution were assessed via non-invasive <i>in vivo</i> SPECT/CT imaging and <i>ex vivo</i> gamma counting. Single administration of RIT achieved a 2.7-log (99.78%) reduction of bacterial burden in the infected joint capsule, had no effect on the infected femur, and resulted in 72.5% reduction of bacterial burden on the infected implant when compared to untreated controls. RIT reduced bacterial burden and inflammation in experimental PJI with no side effects. These findings underscore the potential of RIT in the treatment of infected indwelling devices and warrant further study.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"44 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13150401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-Printed Patient-Specific Guides Versus Conventional Techniques for Scaphoid Reconstruction: A Comparative Study of 89 Cases","authors":"Sylvano Mania,&nbsp;Mauro Maniglio,&nbsp;Michael A. Wirth,&nbsp;Ladislav Nagy,&nbsp;Lisa Reissner,&nbsp;Andreas Schweizer","doi":"10.1002/jor.70220","DOIUrl":"10.1002/jor.70220","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Scaphoid non-union or malunion often produces a three-dimensional (3D) deformity that alters wrist biomechanics. Patient-specific instrumentation (PSI) has been introduced to improve reconstruction accuracy, but quantitative comparisons with conventional free-hand techniques remain limited. We retrospectively analyzed 115 scaphoid reconstructions performed between 2010 and 2019; 89 cases met inclusion criteria (45 PSI, 44 conventional). Distal-fragment rotation (flexion/extension, ulnar/radial inclination, pronation/supination) and translation were quantified using a Cartesian coordinate system centered on the proximal pole. A composite 3D deformity angle was calculated to assess global malalignment. PSI achieved greater absolute correction across several parameters. Sagittal flexion improved by 8.9° with PSI versus 5.8° with conventional reconstruction (<i>p</i> = 0.007 and <i>p</i> = 0.043, respectively). Ulnar inclination improved by 3° with PSI (<i>p</i> = 0.017) but not significantly with the conventional technique. The composite 3D deformity angle decreased by 8.4° with PSI and 6.9° conventionally. Despite a longer preoperative non-union duration in the PSI group, consolidation rates (91% vs 93%) and mean times to union were comparable between techniques. Vascularized grafts showed shorter, but non-significant, consolidation times. Both techniques effectively corrected scaphoid deformity. PSI enabled larger morphological corrections in cases with more severe baseline deformity. These findings suggest that PSI may facilitate correction in complex or long-standing non-unions; however, the clinical relevance of the observed 3D differences and operative efficiency remain to be determined.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"44 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a Tenochondral Ossification Sequence Producing Heterotopic Ossification in Human Achilles Tendinopathy.","authors":"Haiyin Li, Brendan Boyce, Veronica Ulici, David Ciufo, Chike Cao","doi":"10.1002/jor.70226","DOIUrl":"10.1002/jor.70226","url":null,"abstract":"<p><p>Achilles tendinopathy is a common musculoskeletal disorder. A frequent late-stage manifestation is tendon heterotopic ossification (HO). Achilles HO forms predominantly via endochondral ossification, yet the cellular and molecular mechanisms underlying this pathologic condition remain elusive. Here, we analyzed surgically excised biopsy samples with symptomatic human Achilles tendons alongside non-diseased Achilles tendon controls primarily from lower-limb amputation donors to characterize the morphologic and mineralization patterns of the disease. Based on these analyses, we propose a stepwise endochondral model of Achilles HO that we term \"tenochondral ossification\" to emphasize the chondrogenic intermediate stage and its tendon origin. This process includes tendon-to-cartilage metaplasia, characterized by increased glycosaminoglycan content (Alcian blue-positive matrix) and the appearance of chondrocyte-like cells aligning the collagen fibers; some cells have substantial RUNX2 or SOX9 expression; subsequent mineralization of the metaplastic tissue, indicated by alkaline phosphatase immunoreactivity and neovessel ingrowth; osteoclast differentiation and resorption of the calcified metaplastic tissue; and new bone deposition on metaplastic tendon remnants accompanied by robust RUNX2 immunoactivity. Furthermore, TNC expression remains high in the metaplastic cartilage but diminishes at the mineralization front. Taken together, these data support a tendon-origin, endochondral pathway for Achilles tendon HO and highlight stage-specific therapeutic opportunities for treating or preventing Achilles tendinopathy and HO development and progression.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"44 5","pages":"e70226"},"PeriodicalIF":2.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13251765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147959808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen III Deficiency Following Injury in Female Murine Tendons Alters Matrix Composition, Structure, Organization and Function.","authors":"J A Carlson, W Yen, S N Weiss, S W Volk, L J Soslowsky","doi":"10.1002/jor.70227","DOIUrl":"10.1002/jor.70227","url":null,"abstract":"<p><p>Tendons withstand large forces due to an aligned, dense collagen matrix. However, their low cellularity and relative inability to recruit reparative cells post-injury, coupled with a susceptibility to excessive scarring results in loss of tendon structure and function. Type III collagen (COL3) plays a key role in regulating matrix architecture and limiting scar formation following cutaneous injury; however, its role in tendon remains unclear. We examined the impact of reduced COL3 using an established murine Col3a1 knockdown model. Uninjured tendons in Col3a1^+/- mice had a broader distribution of fibrils compared to Col3a1^+/+ mice. Fibrils in injured tendons of Col3a1^+/- mice were larger than those in Col3a1^+/+ mice at 3-weeks post injury but were smaller than their littermates at 6-weeks. Injured Col3a1^+/- tendons had enhanced fiber alignment at 1- and 6-weeks post-injury and an increase in ɑSMA⁺ myofibroblasts at 3-weeks post-injury. Differential expression of matrix components, as well as markers of cells, cell-ECM interaction, and inflammation were discovered. Anti-inflammatory macrophages were decreased in Col3a1^+/- tendons 1-week following injury, with no differences between genotypes later in healing. Pro-inflammatory macrophages remained unchanged between genotypes early in healing, but were increased in Col3a1^+/+ tendons compared to Col3a1^+/- tendons at 3-weeks post-injury. Finally, altered quasistatic mechanical properties was noted in COL3-deficient injured tendons. Our data suggests COL3 plays a complex role in regulating cell phenotype, activity, and fate, as well as collagen matrix architecture in the tendon injury microenvironment, which impacts tendon structure-function post-injury.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"44 6","pages":"e70227"},"PeriodicalIF":2.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13451989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iso-Height Axis Provides More Physiologic Description of Condylar Kinematics and Frontal Plane Stability During Functional Activities After Unicompartmental Knee Arthroplasty","authors":"Tianyun Gu,&nbsp;Chunjie Xia,&nbsp;Huiyong Dai,&nbsp;Huarui Shen,&nbsp;Jingchi Li,&nbsp;Qi Wang,&nbsp;Nan Zheng,&nbsp;Zhiyong Hou,&nbsp;Tsung-Yuan Tsai","doi":"10.1002/jor.70205","DOIUrl":"10.1002/jor.70205","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Accurate characterization of femoral condylar kinematics and its association with collateral ligament behavior are essential for maintaining overall knee stability, particularly frontal plane stability after medial unicompartmental knee arthroplasty (UKA). Conventional reference flexion axes, such as the transepicondylar axis (TEA) and geometric center axis (GCA), provide only static representations of motion. The recently proposed iso-height axis (IHA) offers a dynamic reference, yet its clinical relevance under weight-bearing conditions after UKA remains unclear. 24 patients, 13 fixed-bearing (FB) and 11 mobile-bearing (MB) UKA, underwent dual fluoroscopic imaging (DFIS) during weight-bearing (single-leg lunge, sit-to-stand) and non-weight-bearing (open-chain leg raising) maneuvers. Femoral condyle kinematics were analyzed using TEA, GCA, and IHA, with statistical analysis comparing native and UKA knees. In FB UKA, A-P condylar translation was consistently the smallest with IHA (UKA: 0.78–1.69 mm across tasks), compared with GCA (2.98–3.62 mm) and TEA (2.99–6.03 mm; mean across medial/lateral condyles). FB UKA demonstrated robust correlations between condylar kinematics and ligament changes across all three axes, with IHA uniquely capturing bilateral lateral collateral ligament (LCL) correlations during lunge (lateral: <i>β </i>= 0.031,β<i>* </i>= 0.43; medial: <i>β</i> = 0.028, β<i>* </i>= 0.39). MB UKA had reduced correlations, primarily limited to LCL-TEA during open-chain leg raising. Flexion-axis choice substantially affects the interpretation of femoral condylar kinematics after UKA. Weight-bearing assessment better reveals clinically relevant abnormalities, and IHA may provide a more physiologically meaningful reference than TEA or GCA.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"44 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic Interlimb Asymmetries in Loading and Propulsion During Walking From 2 to 8 Months After Anterior Cruciate Ligament Reconstruction","authors":"Romain Brebels,&nbsp;Guillaume J. Bastien,&nbsp;Fabian Dandois,&nbsp;Bénédicte Schepens,&nbsp;Massimo Penta","doi":"10.1002/jor.70206","DOIUrl":"10.1002/jor.70206","url":null,"abstract":"<div>\u0000 \u0000 <p>Normalization of walking is a rehabilitation goal after anterior cruciate ligament reconstruction (ACLR). However, no consensus is currently established on the kinetic variables supporting rehabilitation. We examined interlimb loading asymmetries and deviations from controls from 2 to 8 months post-ACLR at two different speeds. Ground reaction force of 14 patients with ACLR (23.9 years, 7 females) were assessed at 2-, 4-, 6-, and 8-months post-surgery while walking on instrumented treadmill at 5 and 6 km/h. Gait cycle characteristics, including stance phase durations, peak force, center-of-mass displacement and total power, were compared at each time point with matched controls (<i>t</i>-tests) and between involved and uninvolved limbs (mixed linear model). We observed that kinetic asymmetries were largest at 2 months and most persisted up to 8 months post-ACLR. An unloading strategy was observed on the involved limb, especially at 6 km/h, concerning the loading peak force (−6.6% at 4 months), the braking peak force (−9% at 6 months), the power absorption (−15.7% at 8 months) and the single support duration (−1.8% at 6 months compared to controls). These findings indicate that walking recovery after ACLR is characterized by unloading of the involved limb especially during the loading response and a compensation from the uninvolved limb throughout the stance, lasting up to 8 months when walking at 5 and 6 km/h. Walking at higher, controlled walking speeds should be assessed and retrained early after ACLR to identify and reduce residual loading asymmetries.</p>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"44 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-Articular Purified Exosome Product and Hyaluronan Attenuate Osteoarthritis Progression in a Minimally Invasive Turkey Model","authors":"Xing Lei,&nbsp;Lei Wang,&nbsp;Ramona L. Reisdorf,&nbsp;Maggie A. Brosig,&nbsp;Steven L. Moran,&nbsp;Christopher H. Evans,&nbsp;Chunfeng Zhao","doi":"10.1002/jor.70207","DOIUrl":"10.1002/jor.70207","url":null,"abstract":"<div>\u0000 \u0000 <p>Our study aimed to develop a novel, reproducible, minimally invasive turkey knee osteoarthritis (OA) model and evaluate whether intra-articular hyaluronan (HA) alone or combined with purified exosome product (PEP) attenuates OA-related functional and structural deterioration. OA was induced arthroscopically by transection of the anterior and posterior cruciate ligaments with resection of the anterior horn of the medial meniscus. Animals received intra-articular injections of saline, HA, or PEP + HA at postoperative 2–5 weeks. Gait was assessed at 4, 8, and 12 weeks, and cartilage degeneration, subchondral bone sclerosis, osteophyte formation, synovial fibrosis, heterotopic ossification, and meniscal integrity were evaluated by quantitative CT and histology at week 12. Compared with saline, HA improved lameness, preserved cartilage glycosaminoglycan content, and reduced meniscal rupture, subchondral bone sclerosis, and periarticular heterotopic ossification. PEP + HA provided additional benefits, including prolonged stance phase, reduced synovial fibrosis, and greater chondroprotection than HA alone, while plantar pressure variables, subchondral bone remodeling, and patellar osteophytes did not differ between HA and PEP + HA. This turkey model reliably produces human-like OA changes and enables longitudinal functional and structural assessment. Intra-articular HA mitigated OA progression, and the addition of PEP conferred incremental joint protection.</p></div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"44 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-Linked Versus Linear Hyaluronic Acid for Cartilage Repair in Rat Post-Traumatic Osteoarthritis","authors":"Tien-Ching Lee,&nbsp;Chih-Ming Kao,&nbsp;Yi-Wun Lin,&nbsp;Yi-Shan Lin,&nbsp;Yan-Hsiung Wang,&nbsp;Hsuan-Fu Chen,&nbsp;Yin-Chih Fu","doi":"10.1002/jor.70213","DOIUrl":"10.1002/jor.70213","url":null,"abstract":"<p>Post-traumatic knee osteoarthritis develops following acute joint injuries and can progress rapidly, causing significant morbidity. Intra-articular hyaluronic acid injections are a common treatment, but efficacy varies between formulations. We hypothesized that cross-linked hyaluronic acid would provide superior chondroprotective and anti-inflammatory effects compared to linear hyaluronic acid in a standardized post-traumatic osteoarthritis model. Post-traumatic osteoarthritis was induced via anterior cruciate ligament transection in 84 male Sprague-Dawley rats assigned to four groups: Sham (<i>n</i> = 12), untreated osteoarthritis (<i>n</i> = 24), linear hyaluronic acid—Jetknee (<i>n</i> = 24), or cross-linked hyaluronic acid—HYAJOINT Plus (<i>n</i> = 24). At each of four time points (Day 7, Week 4, Week 8, and Week 12 post-surgery), three Sham and six rats per treatment group were sacrificed. Functional recovery was assessed by weight-bearing distribution. Cartilage integrity was evaluated histologically using hematoxylin and eosin and Safranin O staining, scored with the Osteoarthritis Research Society International histopathological grading system. Immunohistochemical expression of type II collagen, type X collagen, matrix metalloproteinase-13, and tumor necrosis factor-alpha was analyzed. Both hyaluronic acid formulations significantly improved weight-bearing compared to untreated controls. Cross-linked hyaluronic acid demonstrated more sustained functional recovery and superior cartilage preservation with lower histological scores. Immunohistochemistry revealed reduced catabolic and inflammatory marker expression with higher type II collagen in the cross-linked hyaluronic acid group. High molecular weight cross-linked hyaluronic acid conferred stronger and longer-lasting chondroprotective effects than linear hyaluronic acid, suggesting that structural optimization of hyaluronic acid formulations may confer more sustained chondroprotective effects in this preclinical model of post-traumatic osteoarthritis.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"44 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}