Journal of Orthopaedic Research®最新文献_第9页

A translational murine model of aseptic loosening with osseointegration failure 无菌性松动与骨结合失败的小鼠转化模型。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-06-20 DOI: 10.1002/jor.25915

Andrew L. Thomson, Vincentius J. Suhardi, Yingzhen Niu, Anastasia Oktarina, Kevin Döring, Christina Chao, Matthew B. Greenblatt, Lionel B. Ivashkiv, Mathias P. G. Bostrom, Xu Yang

{"title":"A translational murine model of aseptic loosening with osseointegration failure","authors":"Andrew L. Thomson, Vincentius J. Suhardi, Yingzhen Niu, Anastasia Oktarina, Kevin Döring, Christina Chao, Matthew B. Greenblatt, Lionel B. Ivashkiv, Mathias P. G. Bostrom, Xu Yang","doi":"10.1002/jor.25915","DOIUrl":"10.1002/jor.25915","url":null,"abstract":"An in vivo animal model of a weight-bearing intra-articular implant is crucial to the study of implant osseointegration and aseptic loosening caused by osseointegration failure. Osseointegration, defined as a direct structural and functional attachment between living bone tissue and the surface of a load-carrying implant, is essential for implant stability and considered a prerequisite for the long-term clinical success of implants in total joint arthroplasty. Compared to large animal models, murine models offer extensive genetic tools for tracing cell differentiation and proliferation. The 18- to 22-week-old C57BL/6J background mice underwent either press-fitted or loose implantation of a titanium implant, achieving osseointegration or fibrous integration. A protocol was developed for both versions of the procedure, including a description of the relevant anatomy. Samples were subjected to microcomputed tomography and underwent biomechanical testing to access osseointegration. Lastly, samples were fixed and embedded for histological evaluation. The absence of mineralized tissue and weakened maximum pull-out force in loose implantation samples indicated that these implants were less mechanically stable compared to the control at 4 weeks postoperation. Histological analysis demonstrated extensive fibrotic tissue in the peri-implant area of loose implantation samples and excellent implant osseointegration in press-fitted samples at 4 weeks. Both mechanically stable and unstable hemiarthroplasty models with either osseous ingrowth or a robust periprosthetic fibrosis were achieved in mice. We hope that this model can help address current limitations for in vivo study of aseptic loosening and lead to necessary translational benefits.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomics in orthopedic research: Recent studies and their translational implications 骨科研究中的蛋白质组学：最新研究及其转化意义。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-06-19 DOI: 10.1002/jor.25917

George Li, Argyrios Stampas, Yoshihiro Komatsu, Xueqin Gao, Johnny Huard, Sheng Pan

{"title":"Proteomics in orthopedic research: Recent studies and their translational implications","authors":"George Li, Argyrios Stampas, Yoshihiro Komatsu, Xueqin Gao, Johnny Huard, Sheng Pan","doi":"10.1002/jor.25917","DOIUrl":"10.1002/jor.25917","url":null,"abstract":"Proteomics is a growing field that offers insights into various aspects of disease processes and therapy responses. Within the field of orthopedics, there are a variety of diseases that have a poor prognosis due to a lack of targeted curative therapy or disease modifying therapy. Other diseases have been difficult to manage in part due to lack of clinical biomarkers that offer meaningful insight into disease progression or severity. As an emerging technology, proteomics has been increasingly applied in studying bone biology and an assortment of orthopedics related diseases, such as osteoarthritis, osteosarcoma and bone tumors, osteoporosis, traumatic bone injury, spinal cord injury, hip and knee arthroplasty, and fragile healing. These efforts range from mechanistic studies for elucidating novel insights in tissue activity and metabolism to identification of candidate biomarkers for diagnosis, prognosis, and targeted treatment. The knowledge gained from these proteomic and functional studies has provided unique perspectives in studying orthopedic diseases. In this review, we seek to report on the current state of the proteomic study in the field of orthopedics, overview the advances in clinically applicable discoveries, and discuss the opportunities that may guide us for future research.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated quantitative assessment of bone contusions and overlying articular cartilage following anterior cruciate ligament injury 前十字韧带损伤后骨挫伤和上覆关节软骨的自动定量评估。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-06-17 DOI: 10.1002/jor.25920

Allen A. Champagne, Taylor M. Zuleger, Shayla M. Warren, Daniel R. Smith, Joseph D. Lamplot, John W. Xerogeanes, Alexis B. Slutsky-Ganesh, Prathap Jayaram, Jay M. Patel, Gregory D. Myer, Jed A. Diekfuss

{"title":"Automated quantitative assessment of bone contusions and overlying articular cartilage following anterior cruciate ligament injury","authors":"Allen A. Champagne, Taylor M. Zuleger, Shayla M. Warren, Daniel R. Smith, Joseph D. Lamplot, John W. Xerogeanes, Alexis B. Slutsky-Ganesh, Prathap Jayaram, Jay M. Patel, Gregory D. Myer, Jed A. Diekfuss","doi":"10.1002/jor.25920","DOIUrl":"10.1002/jor.25920","url":null,"abstract":"Quantitative methods to characterize bone contusions and associated cartilage injury remain limited. We combined standardized voxelwise normalization and 3D mapping to automate bone contusion segmentation post-anterior cruciate ligament (ACL) injury and evaluate anomalies in articular cartilage overlying bone contusions. Forty-five patients (54% female, 26.4 ± 11.8 days post-injury) with an ACL tear underwent 3T magnetic resonance imaging of their involved and uninvolved knees. A novel method for voxelwise normalization and 3D anatomical mapping was used to automate segmentation, labeling, and localization of bone contusions in the involved knee. The same mapping system was used to identify the associated articular cartilage overlying bone lesions. Mean regional T1ρ was extracted from articular cartilage regions in both the involved and uninvolved knees for quantitative paired analysis against ipsilateral cartilage within the same compartment outside of the localized bone contusion. At least one bone contusion lesion was detected in the involved knee within the femur and/or tibia following ACL injury in 42 participants. Elevated T1ρ (p = 0.033) signal were documented within the articular cartilage overlying the bone contusions resulting from ACL injury. In contrast, the same cartilaginous regions deprojected onto the uninvolved knees showed no ipsilateral differences (p = 0.795). Automated bone contusion segmentation using standardized voxelwise normalization and 3D mapping deprojection identified altered cartilage overlying bone contusions in the setting of knee ACL injury.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141419531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Muscle and joint function in the rotator cuff deficient shoulder 肩袖缺损肩部的肌肉和关节功能。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-06-12 DOI: 10.1002/jor.25909

Angus Yeung, Ashen Fernando, Minoo Patel, Laura Gatto, David C. Ackland

{"title":"Muscle and joint function in the rotator cuff deficient shoulder","authors":"Angus Yeung, Ashen Fernando, Minoo Patel, Laura Gatto, David C. Ackland","doi":"10.1002/jor.25909","DOIUrl":"10.1002/jor.25909","url":null,"abstract":"Full-thickness rotator cuff tears can lead to poor coaptation of the humeral head to the glenoid, disrupting muscle forces required for glenohumeral joint stability, ultimately leading to joint subluxation. The aim of this study was to evaluate muscle forces and glenohumeral joint translations during elevation in the presence of isolated and combined full-thickness rotator cuff tears. Eight fresh-frozen upper limbs were mounted to a computer-controlled testing apparatus that simulated joint motion by simulated muscle force application. Scapular-plane abduction was performed, and glenohumeral joint translations were measured using an optoelectronic system. Testing was performed in the native shoulder, a following an isolated tear to the supraspinatus, as well as combined tears involving the supraspinatus and subscapularis, as well as supraspinatus, infraspinatus, and teres minor. Rotator cuff tears significantly increased middle deltoid force at 30°, 60°, and 90° of abduction relative to that in the native shoulder (p < 0.05). Significantly greater superior translations were observed relative to the intact shoulder due to combined tears to the supraspinatus and infraspinatus at 30° of abduction (mean increase: 1.6 mm, p = 0.020) and 60° of abduction (mean increase: 4.8 mm, p = 0.040). This study illustrates the infraspinatus-teres minor complex as a major humeral head depressor and contributor to glenohumeral joint stability. An increase in deltoid force during abduction occurs in the presence of rotator cuff tears, which exacerbates superior migration of the humeral head. The findings may help in the development of clinical tests in rotator cuff tear diagnostics, in surgical planning of rotator cuff repair, and in planning of targeted rehabilitation.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25909","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triamcinolone acetonide has minimal effect on short- and long-term metabolic activities of cartilage 曲安奈德对软骨的短期和长期代谢活动影响极小。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-06-11 DOI: 10.1002/jor.25913

Annie Porter, Emily Newcomb, Steven DiStefano, Jacob Poplawski, Jonathan Kim, Michael Axe, Xin Lucas Lu

{"title":"Triamcinolone acetonide has minimal effect on short- and long-term metabolic activities of cartilage","authors":"Annie Porter, Emily Newcomb, Steven DiStefano, Jacob Poplawski, Jonathan Kim, Michael Axe, Xin Lucas Lu","doi":"10.1002/jor.25913","DOIUrl":"10.1002/jor.25913","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 Intra-articular corticosteroid injections, such as triamcinolone acetonide (TA), are commonly used by clinicians to manage joint synovial inflammation. However, due to conflicting evidence in literature, there is a fear among clinicians that the injections may be harmful to otherwise healthy cartilage in young patients. The purpose of this study was to evaluate the effects of TA on young, healthy chondrocytes. Articular cartilage samples were harvested from bovine knee joints (1–2 months old). In both healthy and inflammatory (interleukin-1β) challenged cartilage, samples were treated with TA at doses ranging from 1 nM to 200 μM. Following a short- (2 days) or long-term (10–14 days) treatment, chondrocyte viability, proliferation, and extracellular matrix (ECM) synthesis and degradation were evaluated with a click chemistry-based technique. Chondrocyte viability, proliferation, and anabolic activity were all minimally affected by short-term and long-term TA treatment. After both acute and sustained inflammatory challenges, TA reduced the catabolic activities in cartilage, reducing nascent glycosaminoglycan loss and maintaining cartilage mechanical properties. Overall, at physiologically relevant doses, TA had minimal negative impact on chondrocytes when maintained within their native ECM. Clinical significance: The findings provide new insight for current clinical practices concerning the use of TA in intra-articular injections, especially in young patients, and established a foundation for future investigations into the impact of corticosteroids on joint homeostasis.\u0000 </section>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141300805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive gene expression analysis using RNA sequencing between male and female patients with idiopathic carpal tunnel syndrome 利用 RNA 测序对特发性腕管综合征男女患者的基因表达进行综合分析。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-06-10 DOI: 10.1002/jor.25914

Yusuke Ogura, Hiroaki Miyoshi, Shiro Yoshida, Fumiko Arakawa, Mai Takeuchi, Kenjiro Nakama, Mitsuhiro Matsuura, Hirofumi Takada, Yoshiaki Yamanaka, Koji Hiraoka, Koichi Ohshima

{"title":"Comprehensive gene expression analysis using RNA sequencing between male and female patients with idiopathic carpal tunnel syndrome","authors":"Yusuke Ogura, Hiroaki Miyoshi, Shiro Yoshida, Fumiko Arakawa, Mai Takeuchi, Kenjiro Nakama, Mitsuhiro Matsuura, Hirofumi Takada, Yoshiaki Yamanaka, Koji Hiraoka, Koichi Ohshima","doi":"10.1002/jor.25914","DOIUrl":"10.1002/jor.25914","url":null,"abstract":"Idiopathic carpal tunnel syndrome is the most common entrapment neuropathy in hand surgery, and it is characterized by Noninflammatory fibrosis of subsynovial connective tissues. The prevalence and incidence differ between male and female individuals, and the mechanism underlying this difference remains largely unclear. In the present study, we collected subsynovial connective tissues from six male and six female patients diagnosed with idiopathic carpal tunnel syndrome during surgery. We performed a comprehensive gene expression analysis using RNA sequencing to compare the gene expression profiles between male and female patients with idiopathic carpal tunnel syndrome. We identified 26 genes with significantly different expressions between male and female patients, in which POSTN, COL1A1, and COL3A1, which are involved in extracellular matrix organization, and IGF1, an important fibrotic factor, were significantly upregulated in male patients. Immunohistochemistry confirmed the expression of proteins encoded by these genes in tissues, and male patients tended to show increased POSTN expression. Our results indicate that fibrosis of subsynovial connective tissues is induced by different mechanisms in male and female patients, and genes involved in extracellular matrix organization, especially POSTN, might be important factors in male patients. This study provides insight into the pathogenesis of idiopathic carpal syndrome and might contribute to the development of new treatment strategies.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational modeling of revision total hip arthroplasty involving acetabular defects: A systematic review 涉及髋臼缺损的翻修全髋关节置换术的计算建模：系统综述。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-06-08 DOI: 10.1002/jor.25902

Daniel Hopkins, Stuart A. Callary, L. B. Solomon, Sarah C. Woodford, Peter V. S. Lee, David C. Ackland

{"title":"Computational modeling of revision total hip arthroplasty involving acetabular defects: A systematic review","authors":"Daniel Hopkins, Stuart A. Callary, L. B. Solomon, Sarah C. Woodford, Peter V. S. Lee, David C. Ackland","doi":"10.1002/jor.25902","DOIUrl":"10.1002/jor.25902","url":null,"abstract":"Revision total hip arthroplasty (rTHA) involving acetabular defects is a complex procedure associated with lower rates of success than primary THA. Computational modeling has played a key role in surgical planning and prediction of postoperative outcomes following primary THA, but modeling applications in rTHA for acetabular defects remain poorly understood. This study aimed to systematically review the use of computational modeling in acetabular defect classification, implant selection and placement, implant design, and postoperative joint functional performance evaluation following rTHA involving acetabular defects. The databases of Web of Science, Scopus, Medline, Embase, Global Health and Central were searched. Fifty-three relevant articles met the inclusion criteria, and their quality were evaluated using a modified Downs and Black evaluation criteria framework. Manual image segmentation from computed tomography scans, which is time consuming, remains the primary method used to generate 3D models of hip bone; however, statistical shape models, once developed, can be used to estimate pre-defect anatomy rapidly. Finite element modeling, which has been used to estimate bone stresses and strains, and implant micromotion postoperatively, has played a key role in custom and off-the-shelf implant design, mitigation of stress shielding, and prediction of bone remodeling and implant stability. However, model validation is challenging and requires rigorous evaluation and comparison with respect to mid- to long-term clinical outcomes. Development of fast, accurate methods to model acetabular defects, including statistical shape models and artificial neural networks, may ultimately improve uptake of and expand applications in modeling and simulation of rTHA for the research setting and clinic.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25902","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information - Editorial Board and TOC 期刊信息 - 编辑委员会和目录

IF 2.8 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-06-06 DOI: 10.1002/jor.25612

引用次数: 0

Issue Information - Cover 发行信息 - 封面

IF 2.8 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-06-06 DOI: 10.1002/jor.25613

引用次数: 0

Beyond weakness: Exploring intramuscular fat and quadriceps atrophy in ACLR recovery 超越虚弱：探索前交叉韧带损伤恢复过程中的肌肉内脂肪和股四头肌萎缩。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-06-01 DOI: 10.1002/jor.25910

McKenzie S. White, Augustin C. Ogier, Thomas L. Chenevert, Elizabeth Zucker, Luke Stoneback, Constance P. Michel, Riann M. Palmieri-Smith, Lindsey K. Lepley

{"title":"Beyond weakness: Exploring intramuscular fat and quadriceps atrophy in ACLR recovery","authors":"McKenzie S. White, Augustin C. Ogier, Thomas L. Chenevert, Elizabeth Zucker, Luke Stoneback, Constance P. Michel, Riann M. Palmieri-Smith, Lindsey K. Lepley","doi":"10.1002/jor.25910","DOIUrl":"10.1002/jor.25910","url":null,"abstract":"Muscle weakness following anterior cruciate ligament reconstruction (ACLR) increases the risk of posttraumatic osteoarthritis (OA). However, focusing solely on muscle weakness overlooks other aspects like muscle composition, which could hinder strength recovery. Intramuscular fat is a non-contractile element linked to joint degeneration in idiopathic OA, but its role post-ACLR has not been thoroughly investigated. To bridge this gap, we aimed to characterize quadriceps volume and intramuscular fat in participants with ACLR (male/female = 15/9, age = 22.8 ± 3.6 years, body mass index [BMI] = 23.2 ± 1.9, time since surgery = 3.3 ± 0.9 years) and in controls (male/female = 14/10, age = 22.0 ± 3.1 years, BMI = 23.3 ± 2.6) while also exploring the associations between intramuscular fat and muscle volume with isometric strength. Linear mixed effects models assessed (I) muscle volume, (II) intramuscular fat, and (III) strength between limbs (ACLR vs. contralateral vs. control). Regression analyses were run to determine if intramuscular fat or volume were associated with quadriceps strength. The ACLR limb was 8%−11% smaller than the contralateral limb (p < 0.05). No between-limb differences in intramuscular fat were observed (p = 0.091−0.997). Muscle volume but not intramuscular fat was associated with strength in the ACLR and control limbs (p < 0.001−0.002). We demonstrate that intramuscular fat does not appear to be an additional source of quadriceps dysfunction following ACLR and that muscle size only explains some of the variance in muscle strength.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0