Journal of Orthopaedic Research®最新文献

{"title":"Bone and Nerve Response to Sciatic Compression Neuropathy in a Rabbit Model","authors":"Anthony R. Fringuello,&nbsp;Joey S. Kurtzman,&nbsp;Westley Hayes,&nbsp;John Carter,&nbsp;Steven M. Koehler","doi":"10.1002/jor.26045","DOIUrl":"10.1002/jor.26045","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Compression neuropathy is a prevalent medical condition, including common types such as carpal tunnel syndrome, cubital tunnel syndrome, sciatica, and many others. While the neurological consequences are well understood, the effects on bone properties and the potential downstream impact on fracture risk remain less clear. This study aimed to assess the influence of compressive neuropathy on bone properties using a rabbit model of sciatic nerve compression. We hypothesized that compressive neuropathy could adversely alter bone properties. Five New Zealand white rabbits underwent surgery to induce perineural scarring in the sciatic nerve, with the contralateral limb serving as a sham control. Bone mineral density (BMD), mechanical strength, and bone signaling proteins were evaluated through microcomputed tomography (μCT), four-point bending tests, and ELISA assays, respectively. Sciatic nerve histology was analyzed using VEGF and Nissl staining to assess axon and Schwann cell densities and quantified using image analysis software. The results showed no significant differences in BMD, biomechanical properties, or key bone signaling proteins (OPG and RANKL) between the affected and control tibias. These findings suggest that compression neuropathy does not significantly impact bone properties in the rabbit model.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 4","pages":"803-809"},"PeriodicalIF":2.1,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Postoperative Neck-Shaft and Anteversion Angles on Biomechanical Outcomes in Proximal Femoral Osteotomy: An In Silico Study","authors":"Alireza Y. Bavil,&nbsp;Emmanuel Eghan-Acquah,&nbsp;Laura E. Diamond,&nbsp;Rod Barrett,&nbsp;David Bade,&nbsp;Christopher P. Carty,&nbsp;Stefanie Feih,&nbsp;David J. Saxby","doi":"10.1002/jor.26043","DOIUrl":"10.1002/jor.26043","url":null,"abstract":"<div>\u0000 \u0000 <p>Effective surgical planning is crucial for maximizing patient outcomes following complex orthopedic procedures such as proximal femoral osteotomy. In silico simulations can be used to assess how surgical variations in proximal femur geometry, such as femur neck-shaft and anteversion angles, affect postoperative system mechanics. This study investigated the sensitivity of femur mechanics to postoperative neck-shaft angles, anteversion angles, and osteotomy contact areas using patient-specific finite element analysis informed by neuromusculoskeletal models. A sequential neuromusculoskeletal modeling and finite element analysis pipeline was used to simulate postoperative mechanics in three pediatric patients with varying demographic and anatomic features. Nine surgical configurations derived from permutations of the clinical envelope of neck-shaft angles and anteversion angles were simulated for the stance phase of gait. The outcome mechanics assessed were peak von Mises stresses on the bone-implant contact surfaces as well as interfragmentary movement and strain on the osteotomy location. Peak von Mises stress and interfragmentary movement and strain were on average 38% more sensitive to surgical variation in neck-shaft angle compared to anteversion angle. A significant negative correlation was detected between contact area and interfragmentary movement (<i>r</i> = −0.90, <i>p</i> &lt; 0.0001) and strain (<i>r</i> = −0.45, <i>p</i> = 0.017). Overall findings suggest neck-shaft angle significantly influences postoperative femur mechanics and highlight the importance of maximizing contact area to limit interfragmentary motion and foster an optimal mechanical environment for bone healing and callus formation following proximal femoral osteotomy. Between-patient variation in sensitivity to proximal femoral geometry reinforced the importance of patient-specific surgical planning.</p></div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 4","pages":"842-852"},"PeriodicalIF":2.1,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kartogenin Enhances Chondrogenic Differentiation of iPSC Derived MSCs (iMSCs) and Improves Outcomes in an Osteochondral Defect Model in Male Rats","authors":"Samir Chihab,&nbsp;Nazir M. Khan,&nbsp;Tracy Eng,&nbsp;Thanh Doan,&nbsp;Jarred M. Kaiser,&nbsp;Hicham Drissi","doi":"10.1002/jor.26040","DOIUrl":"10.1002/jor.26040","url":null,"abstract":"<div>\u0000 \u0000 <p>Osteochondral defects (OCD) pose a significant clinical challenge due to the limited self-repair capacity of cartilage, leading to pain, joint dysfunction, and progression to osteoarthritis. Cellular implantations of adult mesenchymal stem cells (MSCs) enhanced with treatment of factors, such as small molecule Kartogenin (KGN) to promote chondrogenic differentiation, are promising but these cells often encounter hypertrophy during differentiation, compromising long-term stability. Induced pluripotent stem cell-derived MSCs (iMSCs) offer greater proliferative and differentiation capacity than MSCs and may provide a superior source of cells for cartilage repair. We hypothesized that treatment of iMSCs with TGFβ3 and KGN would enhance chondrogenic differentiation and that implanting these pellets into a rat OCD model would promote de novo cartilage regeneration and reduce pain behavior. We pellet cultured iMSCs derived from articular chondrocytes and treated with various conditions of TGFβ3 and KGN. We then assessed the in vivo performance of the pellets using a trochlear osteochondral defect in male Lewis rats. Co-treatment of iMSC pellets with TGFβ3 and KGN showed more pronounced chondrogenic differentiation than sequential treatment and exhibited stronger expression of chondrogenic genes. Implantation of the TGFβ3/KGN-treated iMSC pellets into OCD resulted in modest repair, as observed via gross morphology, effectively prevented the onset of joint hyperalgesia, and helped to maintain normal gait out to 12 weeks post-implantation compared to untreated OCD rats. Our study highlights the potential of KGN to enhance iMSC pellet chondrogenesis, offering a scaffold-free, cell-based therapy that could simplify clinical translation and improve outcomes for patients with cartilage injuries.</p></div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 4","pages":"870-880"},"PeriodicalIF":2.1,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Muscle Contraction Is Essential for Tendon Healing and Muscle Function Recovery After Achilles Tendon Rupture and Surgical Repair","authors":"Moe Yoneno,&nbsp;Yuki Minegishi,&nbsp;Haruna Takahashi,&nbsp;Kei Takahata,&nbsp;Himari Miyamoto,&nbsp;Yuna Usami,&nbsp;Takanori Kokubun","doi":"10.1002/jor.26044","DOIUrl":"10.1002/jor.26044","url":null,"abstract":"<p>Incomplete tendon healing and postponed muscle weakness after Achilles tendon rupture and surgical repair lead to poor performance in patient activities. Although the effectiveness of postoperative early functional rehabilitation has been proven, the priority and each effect of specific methods in early rehabilitation remain unclear. We hypothesized early muscle contraction exercises without joint motion would promote tendon healing and prevent calf muscle atrophy; in contrast, early static stretching after surgical repair would not contribute to tendon healing and induce calf muscle atrophy. C57Bl/6 mice underwent Achilles tendon rupture and suture repair, followed by different methods of post-surgery interventions: a non-exercise group, a Static stretching group, and an Electrical muscle stimulation group. 3 and 5 weeks after surgery, we assessed ex vivo tendon mechanical properties, collagen fiber alignment, and histological muscle properties. Electrical Muscle Stimulation restored the recovery of tendon mechanical properties and muscle strength more quickly than Static stretching. Static stretching had no additional effect on them compared to the non-exercise. Our results suggested that calf muscle contraction was essential as a post-surgery early functional rehabilitation to load tensile forces on tendons and improve Achilles tendon healing. Additionally, early muscle contractions naturally promote restoring muscle function after the rupture, but further research is needed to optimize muscle contraction protocols.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 4","pages":"746-755"},"PeriodicalIF":2.1,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.26044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Mouse Model of Enthesis-Specific NF-κB Activation","authors":"McKenzie E. Sup,&nbsp;Adam C. Abraham,&nbsp;Min Kyu M. Kim,&nbsp;Stavros Thomopoulos","doi":"10.1002/jor.26035","DOIUrl":"10.1002/jor.26035","url":null,"abstract":"<div>\u0000 \u0000 <p>Enthesitis, or inflammation specific to sites in the body where tendon inserts into bone, can arise in isolated joints from overuse or in multiple joints as a complication of an autoimmune condition such as psoriatic arthritis or spondyloarthritis. However, the pathogenesis of enthesitis is not well understood, so treatment strategies are limited. A clinically relevant animal model of enthesitis would allow investigators to determine mechanisms driving the disease and evaluate novel therapies. Therefore, we developed a murine model of inducible enthesis-specific inflammation by constitutively activating the NF-κB pathway in Gli1+ cells. Gli1Cre^ERT mice were crossed with IKKβ-overexpression mice and given tamoxifen injections 5 days postnatally to induce enthesitis. Sixteen weeks of IKKβ overexpression in enthesis cells led to impaired mechanical properties, subtle histologic changes, and changes to expression of extracellular matrix- and inflammation-related genes. Increased loading from treadmill overuse activity did not exacerbate this phenotype. Clinical significance: The new murine model may have utility for studying the pathogenesis of enthesitis and approaches to treat the condition.</p>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 4","pages":"719-727"},"PeriodicalIF":2.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Isotropic Optical Fiber: Antimicrobial Effect of Blue Light on Drug Resistant Organisms","authors":"Megan H. Goh,&nbsp;Robert A. Rabiner,&nbsp;Joseph J. Connolly,&nbsp;Santiago A. Lozano-Calderon,&nbsp;Antonia F. Chen","doi":"10.1002/jor.26042","DOIUrl":"10.1002/jor.26042","url":null,"abstract":"<div>\u0000 \u0000 <p>Drug-resistant organisms (DROs) necessitate the development of new therapies. Antimicrobial blue light (ABL) is a promising option, utilizing photoexcitation of endogenous bacterial components to generate reactive oxygen species, leading to bacterial death. The aim of this study is to investigate the effects of a novel isotropic optical fiber under in-vitro conditions on multidrug-resistant gram-negative <i>Pseudomonas aeruginosa</i> (MDR-Pa) and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). Time-to-kill assays were conducted in tubes containing 10 mL of 0.9% NaCl solution with an inoculum of 1 × 10⁵ CFU/mL for MDR-Pa or MRSA. The experiments were repeated at least three times per strain. Experimental tubes had either one (low power, LP) or two (high power, HP) optical fibers delivering five ABL wavelengths (405, 415, 435, 450, and 475 nm) over 60 min. Control tubes lacked optical fibers. Samples were taken at 0, 10, 20, 30, and 60 min, streaked on agar, and incubated to determine CFU/mL. Bactericidal reduction was defined as a ≥ 99.9% (≥ 3 log₁₀) reduction in CFU/mL. One-way ANOVA were conducted. The novel isotropic optical fiber was able to exhibit bactericidal effects for MDR-Pa only under HP-ABL with a log₁₀CFU/mL ± SD difference of −3.71 ± 0.01 at 60 min (<i>p</i> = 0.03). Conversely, the optical fiber exhibited bactericidal effects on MRSA under both LP-ABL and HP-ABL with a log₁₀CFU/mL±SD difference of −3.73 ± 0.08 at 60 min (<i>p</i> = 0.03) and −3.07 ± 0.28 at 20 min (<i>p</i> = 0.02), respectively. The isotropic optical fiber demonstrated bactericidal effects on MRSA and MDR-Pa in in-vitro studies and shows potential as a therapeutic option for DROs.</p></div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 4","pages":"881-888"},"PeriodicalIF":2.1,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Disulfidptosis-Related Risk Signature for Prognostic Prediction in Patients With Ewing Sarcoma","authors":"Chunqing Che,&nbsp;Delei Song,&nbsp;Peng Xue,&nbsp;Xuqing Yin","doi":"10.1002/jor.26033","DOIUrl":"10.1002/jor.26033","url":null,"abstract":"<div>\u0000 \u0000 <p>Ewing sarcoma (ES) is a malignant bone tumor prevalent among children and adolescents. Disulfidptosis represents a novel form of cell death; however, the mechanism of disulfidptosis in ES remains unclear. Our aim is to explore the disulfidptosis-related prognostic signature in ES. Utilizing transcriptomic and clinical data of ES, disulfidptosis-related hub genes (DRHGs) were identified by differential gene expression analysis and Least Absolute Shrinkage and Selection Operator (LASSO) Cox regression analysis. A disulfidptosis-related risk score model (DRRS) was constructed based on these DRHGs. The performance of DRRS was assessed using survival analysis and receiver operating characteristic curve analysis. Immune cell infiltration in different risk subgroups and correlations between DRRS and antitumor reagents were also analyzed. In this study, we developed a disulfidptosis-related prognostic feature based on <i>LRPPRC</i> (leucine rich pentatricopeptide repeat containing), <i>IQGAP1</i> (IQ motif containing GTPase activating protein 1), <i>NDUFS1</i> (NADH:ubiquinone oxidoreductase core subunit S1), and <i>TLN1</i> (talin 1), which may serve as a predictive and independent risk factor for ES. ES patients in the high-risk group exhibited a poorer prognosis, had a higher proportion of myeloid-derived suppressor cells (MDSCs) and M2 type of tumor-associated macrophages, and showed heightened sensitivity to some antitumor agents such as nilotinib and olaparib. This study is the first to construct a disulfidptosis-related prognostic signature that may predict the prognosis and immune response in ES patients, thereby providing a new reference for understanding the mechanisms of ES and guiding immunotherapy.</p>\u0000 </div>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 4","pages":"790-802"},"PeriodicalIF":2.1,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information - Cover","authors":"","doi":"10.1002/jor.25881","DOIUrl":"https://doi.org/10.1002/jor.25881","url":null,"abstract":"","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25881","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Achilles Tendon Surgical Repair Partially Restores Early Plantar Flexor Structure and Function in a Rat Model","authors":"Ahmad Hammo,&nbsp;Liala Sofi,&nbsp;Lorraine A. T. Boakye,&nbsp;Josh R. Baxter","doi":"10.1002/jor.26041","DOIUrl":"https://doi.org/10.1002/jor.26041","url":null,"abstract":"<p>Achilles tendon ruptures significantly impair long-term patient function, with two-thirds of patients experiencing persistent functional deficits. Although nonsurgical treatment has gained popularity due to its perceived lower risk of complications, the specific effects of this approach on tendon healing, muscle function, and overall performance remain poorly understood. Directly comparing surgical and nonsurgical treatment options in a clinical population is challenging given the diverse nature of the patient population. Preclinical models are essential to isolate the mechanisms underlying these treatments, enabling a detailed examination of the structural and functional outcomes that are difficult to assess in human studies. Here, we surgically induced Achilles tendon ruptures in 20 adult male Sprague Dawley rats and repaired the rupture in half of these animals. Then, functional outcomes were assessed by measuring plantar flexor torque across the ankle's range of motion using a custom-developed small animal dynamometer, and structural changes were evaluated through measurements of Achilles tendon elongation and plantar flexor muscle mass. We found that surgical treatment led to 11%–35% increased functional plantar flexor torque outcomes compared to nonsurgical treatment. Additionally, plantar flexor muscle mass decreased by 21% in nonsurgically treated animals compared to only 12% in the surgically treated group. Our results suggest that surgically repairing a tendon rupture restores plantar flexor function more effectively than nonsurgical treatment; however, persistent functional deficits in both groups indicate that enhanced rehabilitation strategies are necessary for full functional restoration.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 4","pages":"739-745"},"PeriodicalIF":2.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.26041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information - Editorial Board and TOC","authors":"","doi":"10.1002/jor.25880","DOIUrl":"https://doi.org/10.1002/jor.25880","url":null,"abstract":"","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"43 2","pages":"239-242"},"PeriodicalIF":2.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jor.25880","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}