Journal of Orthopaedic Research®最新文献_第2页

Time-dependent changes in medial meniscus kinematics and attachment strength after anterior root injury and repair in a large animal model. 大型动物模型前根损伤和修复后内侧半月板运动学和附着强度随时间的变化。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-11-25 DOI: 10.1002/jor.26022

Kyle D Meadows, John M Peloquin, Madeline Boyes, Brendan D Stoeckl, Jamie Benson, Sonia Bansal, David R Steinberg, Miltiadis H Zgonis, Thomas P Schaer, Robert L Mauck, Dawn M Elliott

{"title":"Time-dependent changes in medial meniscus kinematics and attachment strength after anterior root injury and repair in a large animal model.","authors":"Kyle D Meadows, John M Peloquin, Madeline Boyes, Brendan D Stoeckl, Jamie Benson, Sonia Bansal, David R Steinberg, Miltiadis H Zgonis, Thomas P Schaer, Robert L Mauck, Dawn M Elliott","doi":"10.1002/jor.26022","DOIUrl":"https://doi.org/10.1002/jor.26022","url":null,"abstract":"This study investigated joint kinematics and attachment tensile mechanics following resection of the medial meniscus anterior attachment. A secondary objective investigated the repair of the attachment. Yucatan minipigs underwent unilateral surgery for either Injury (en bloc) resection of the anterior attachment of the insertional ligament, (a portion of the cranial medial meniscotibial ligament) or Repair (immediate repair with a suture anchor), with the contralateral knee as Intact control. Evaluation at 6 weeks and 6 months included joint kinematics measured from MRI acquired under knee compression and tensile testing of the attachment. Injury resulted in large levels of meniscus extrusion, despite the development of a fibrovascular scar. At 6 weeks, the meniscus extruded 1.95 mm more than Intact; at 6 months, this extrusion was reduced to 0.77 mm. Under an applied 1× body weight load, the meniscus further extruded and was not different with treatment or time. During attachment tensile testing, elongation was 0.6 mm for Intact, following Injury, elongation was 2.7 mm at 6 weeks and was partially restored to 1.5 mm at 6 months. Despite this, the cartilage wear worsened over time. Repair was inadequate to avoid the extrusion or cartilage wear seen in the injury group at 6 weeks, so it was not continued for the 6-month group. This study demonstrates that while meniscus injury is useful to study cartilage degeneration, a holistic consideration of the role of the meniscus itself, including its changing material properties and its impact on joint mechanics during injury, repair, and rehabilitation, are key factors contributing to overall joint health.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leveraging AI models for lesion detection in osteonecrosis of the femoral head and T1-weighted MRI generation from radiographs. 利用人工智能模型检测股骨头坏死的病变，并根据射线照片生成 T1 加权磁共振成像。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-11-23 DOI: 10.1002/jor.26026

Issei Shinohara, Atsuyuki Inui, Katherine Hwang, Masatoshi Murayama, Yosuke Susuki, Tomohiro Uno, Qi Gao, Mayu Morita, Simon Kwoon-Ho Chow, Masanori Tsubosaka, Yutaka Mifune, Tomoyuki Matsumoto, Ryosuke Kuroda, Stuart B Goodman

{"title":"Leveraging AI models for lesion detection in osteonecrosis of the femoral head and T1-weighted MRI generation from radiographs.","authors":"Issei Shinohara, Atsuyuki Inui, Katherine Hwang, Masatoshi Murayama, Yosuke Susuki, Tomohiro Uno, Qi Gao, Mayu Morita, Simon Kwoon-Ho Chow, Masanori Tsubosaka, Yutaka Mifune, Tomoyuki Matsumoto, Ryosuke Kuroda, Stuart B Goodman","doi":"10.1002/jor.26026","DOIUrl":"https://doi.org/10.1002/jor.26026","url":null,"abstract":"This study emphasizes the importance of early detection of osteonecrosis of the femoral head (ONFH) in young patients on long-term glucocorticoid therapy, including those with acute lymphoblastic leukemia, lupus, and other diagnoses. While X-ray and magnetic resonance imaging (MRI) are standard imaging methods for staging ONFH, MRI can be costly and time-consuming. The research focuses on utilizing artificial intelligence (AI) to enhance the evaluation of radiographic images for ONFH detection. The study involved analyzing X-ray and MRI from 102 control hips and 104 ONFH-affected hips at Association Research Circulation Osseous (ARCO) Stage II and IIIa. We employed transfer learning with the YOLOv8 model for object detection, using 80% of the data for training and 20% for validation, then assessed detection accuracy through mean average precision (mAP) and a precision-recall curve. Additionally, AI generated synthetic MRI (sMRI) from X-ray images using a Generative Adversarial Network (GAN) and evaluated their similarity to original MRI. Results showed that the mAP for ONFH detection was 0.923 for the YOLOv8n model and 0.951 for YOLOv8x. The GAN-generated sMRI exhibited lower image quality compared with originals but maintained potential for lesion assessment. Intrarater reliability among evaluators was high. The findings indicate that AI techniques, particularly YOLOv8 for object detection and GAN for image generation, can effectively assist in ONFH screening, despite some limitations in the generated MRI quality.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel plate design for rotational guided growth: An experimental study in immature porcine femurs. 用于旋转引导生长的新型钢板设计：未成熟猪股骨的实验研究。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-11-21 DOI: 10.1002/jor.26019

Ahmed Halloum, Ole Rahbek, Shima Gholinezhad, Søren Kold, John Rasmussen, Jan Duedal Rölfing, Maria Tirta, Marie Arildsen, Ahmed A Abood

{"title":"A novel plate design for rotational guided growth: An experimental study in immature porcine femurs.","authors":"Ahmed Halloum, Ole Rahbek, Shima Gholinezhad, Søren Kold, John Rasmussen, Jan Duedal Rölfing, Maria Tirta, Marie Arildsen, Ahmed A Abood","doi":"10.1002/jor.26019","DOIUrl":"https://doi.org/10.1002/jor.26019","url":null,"abstract":"Guided growth for correction of rotational deformities has been reported in several preclinical and clinical studies. Different adverse effects, like growth retardation, articular deformities, and rebound effect have been reported. We have tested a novel plate concept (RotOs PlateTM) intended for the correction of rotational deformities of long bones by guided growth in a porcine model. The plate has sliding screw holes intended to allow for longitudinal growth. Fourteen skeletally immature female pigs were included in the study in a paired design. Mean duration of intervention was 88 days (83-98). CTscans and X-rays were performed at plate insertion and removal. From the CTscans, 3D-models of the left and right femora were made and used for measuring the achieved rotation. Three pigs were excluded for reasons not related to the plate design. The plates rotated as intended in all pigs. In two pigs, there was a cut-out of the proximal screw on the lateral side. Data from these two pigs were included in the results. We observed a mean difference in rotation between the left and right femur of 5.7° in the external direction (CI: 3.7°-7.7°). No statistically significant deformities in the coronal and sagittal plane were observed. The plate worked as intended, that is, the intended rotation was achieved. CLINICAL SIGNIFICANCE: This large animal study shows promising results for the feasibility of the method. It is an important first step in validating the technique and detecting possible adverse effects before clinical studies.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interference fit of cementless tibial implants and the initial mechanical environment: A micro-CT and DVC study. 无骨水泥胫骨植入物的干扰配合与初始机械环境：显微 CT 和 DVC 研究。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-11-20 DOI: 10.1002/jor.26003

Lauren S Wearne, Sophie Rapagna, Greg Keene, Mark Taylor, Egon Perilli

{"title":"Interference fit of cementless tibial implants and the initial mechanical environment: A micro-CT and DVC study.","authors":"Lauren S Wearne, Sophie Rapagna, Greg Keene, Mark Taylor, Egon Perilli","doi":"10.1002/jor.26003","DOIUrl":"https://doi.org/10.1002/jor.26003","url":null,"abstract":"During cementless total knee arthroplasty (TKA), an overlap between the resected tibia and the implant's geometry, termed interference fit, is introduced to facilitate primary stability and direct bone-implant contact. However, little is known about the actual interference achieved and the resulting mechanical response in the surrounding cancellous bone. The aim of this study was (1) to experimentally quantify the actual interference achieved for a commercially available cementless tibial implant and (2) to assess its effect on the post-impaction cancellous bone strain. Seven human cadaveric tibiae were micro-CT scanned intact (23 µm/pixel), once prepared for TKA (46 µm/pixel) and following implantation (46 µm/pixel). The actual interference across the entire bone-implant interface was quantified and, via digital volume correlation, the compressive strains of bone in contact with the implant and at increasing distance, were extracted. An inhomogeneous actual interference was found across the implant pegs and keel (median ± std dev: 0.70 ± 0.27 mm), which was lower than the intended. Limited interference (0.02 ± 0.12 mm) was found directly under the baseplate, with immediate bone-baseplate contact of 54%. The induced compressive strains were related to the actual interference within 3.14 mm from the bone-implant interface (R2 = 0.269-0.450, p < 0.001), with higher compressive strains corresponding to higher interference, but not being related to the bone volume fraction. Clinical Significance: Insight is provided into the interaction between the variability of the resection and the resulting mechanical environment. A complex relationship is apparent, whereby the actual interference accounted for up to 45% of the variation in induced compressive strain magnitude.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-based prediction of contralateral knee osteoarthritis development using the Osteoarthritis Initiative and the Multicenter Osteoarthritis Study dataset. 利用骨关节炎倡议（Osteoarthritis Initiative）和多中心骨关节炎研究（Multicenter Osteoarthritis Study）数据集，基于机器学习预测对侧膝关节骨关节炎的发展。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-11-18 DOI: 10.1002/jor.26018

Ji-Sahn Kim, Byung Sun Choi, Sung Eun Kim, Yong Seuk Lee, Do Weon Lee, Du Hyun Ro

{"title":"Machine learning-based prediction of contralateral knee osteoarthritis development using the Osteoarthritis Initiative and the Multicenter Osteoarthritis Study dataset.","authors":"Ji-Sahn Kim, Byung Sun Choi, Sung Eun Kim, Yong Seuk Lee, Do Weon Lee, Du Hyun Ro","doi":"10.1002/jor.26018","DOIUrl":"10.1002/jor.26018","url":null,"abstract":"Having osteoarthritis in one knee is reported as an independent risk factor for developing contralateral knee osteoarthritis (KOA). However, no study has been designed to predict the development of contralateral KOA (cKOA). The authors hypothesized that specific risk factors for cKOA development exist and that it could be accurately predicted with the assistance of machine learning. KOA was defined using the Kellgren-Lawrence grade (KLG) of 2 or higher. Data from 1353 unilateral KOA patients (900 from the Osteoarthritis Initiative [OAI] and 453 from the Multicenter Osteoarthritis Study [MOST]) over 4-5 years of follow-up were examined. The risk factors for cKOA development were analyzed, and a machine learning model was developed to predict cKOA using OAI as the development data set and MOST as the test data set. cKOA developed in 172 (19.1%) and 178 (39.3%) of the patients (OAI and MOST, respectively) over a period of 4-5 years. A machine learning model was developed using the Tree-based Pipeline Optimization Tool algorithm. This model utilized nine variables, including baseline lateral joint space narrowing grade of the contralateral knee (odds ratio 4.475). The area under the curve of the receiver operating characteristics curve, along with accuracy, precision, and F1-score, were recorded as 0.69, 0.60, 0.50, and 0.58, respectively, in the test data set. The development of cKOA could be effectively predicted using a limited number of variables through machine learning. Surgeons should consider the development of cKOA in patients with identified risk factors when managing KOA patients.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk factors for patellofemoral joint osteoarthritis following ACL reconstruction: A cluster analysis of anatomy and alignment. 前交叉韧带重建术后髌股关节骨关节炎的风险因素：解剖和对齐的聚类分析。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-11-18 DOI: 10.1002/jor.26014

Thomas Demirjian, John Crues, Christopher M Powers

{"title":"Risk factors for patellofemoral joint osteoarthritis following ACL reconstruction: A cluster analysis of anatomy and alignment.","authors":"Thomas Demirjian, John Crues, Christopher M Powers","doi":"10.1002/jor.26014","DOIUrl":"https://doi.org/10.1002/jor.26014","url":null,"abstract":"Individuals who undergo anterior cruciate ligament reconstruction are at elevated risk for developing early-onset patellofemoral joint osteoarthritis. Our objective was to use K-means clustering to ascertain whether individuals at risk for patellofemoral joint osteoarthritis could be identified as determined by the presence of multiple co-existing anatomical and patella alignment risk factors. Forty participants (20 after anterior cruciate ligament reconstruction, 20 healthy controls) underwent magnetic resonance imaging assessment of the patellofemoral joint. Measures of hypothesized risk factors for patellofemoral joint osteoarthritis were obtained including patella alignment (lateral patella displacement and tilt), trochlear morphology (sulcus angle, lateral inclination angle), patella height (Insall-Salvati ratio and patella articulating overlap), and patellofemoral joint contact area. K-means clustering (k = 2) was used to ascertain whether a high-risk group could be identified. Following clustering, two distinct groups were detected. Participants assigned to cluster 1 exhibited features consistent with patellofemoral joint osteoarthritis including greater lateral patellar displacement and tilt, flatter trochlear grooves and lower lateral trochlear inclination, less patella articulating overlap, and reduced contact area. The proportion of females after anterior cruciate ligament reconstruction assigned to cluster 1 was 75% (N = 15) compared to 25% of healthy females (N = 5). K-means clustering was capable of characterizing individuals at elevated risk for patellofemoral joint osteoarthritis based on the presence of multiple co-existing anatomical and patella alignment risk factors. The fact that a significant percentage of females were assigned to the high-risk cluster supports the clinical observation that these individuals may be at higher risk of early-onset patellofemoral joint osteoarthritis.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polytrauma impairs fracture healing accompanied by increased persistence of innate inflammatory stimuli and reduced adaptive response. 多发性创伤会影响骨折愈合，并伴随先天性炎症刺激的持续性增加和适应性反应的降低。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-11-17 DOI: 10.1002/jor.26015

Augustine Mark Saiz, Maryam Rahmati, Robert Charles Henry Gresham, Tony Daniel Baldini, Jane Burgan, Mark A Lee, Benjamin Osipov, Blaine A Christiansen, Thaqif El Khassawna, D C Florian Wieland, André Lopes Marinho, Clement Blanchet, Molly Czachor, Zachary M Working, Chelsea S Bahney, J Kent Leach

{"title":"Polytrauma impairs fracture healing accompanied by increased persistence of innate inflammatory stimuli and reduced adaptive response.","authors":"Augustine Mark Saiz, Maryam Rahmati, Robert Charles Henry Gresham, Tony Daniel Baldini, Jane Burgan, Mark A Lee, Benjamin Osipov, Blaine A Christiansen, Thaqif El Khassawna, D C Florian Wieland, André Lopes Marinho, Clement Blanchet, Molly Czachor, Zachary M Working, Chelsea S Bahney, J Kent Leach","doi":"10.1002/jor.26015","DOIUrl":"10.1002/jor.26015","url":null,"abstract":"The field of bone regeneration has primarily focused on investigating fracture healing and nonunion in isolated musculoskeletal injuries. Compared to isolated fractures, which frequently heal well, fractures in patients with multiple bodily injuries (polytrauma) may exhibit impaired healing. While some papers have reported the overall cytokine response to polytrauma conditions, significant gaps in our understanding remain in how fractures heal differently in polytrauma patients. We aimed to characterize fracture healing and the temporal local and systemic immune responses to polytrauma in a murine model of polytrauma composed of a femur fracture combined with isolated chest trauma. We collected serum, bone marrow from the uninjured limb, femur fracture tissue, and lung tissue over 3 weeks to study the local and systemic immune responses and cytokine expression after injury. Immune cell distribution was assessed by flow cytometry. Fracture healing was characterized using microcomputed tomography (microCT), histological staining, immunohistochemistry, mechanical testing, and small angle X-ray scattering. We detected more innate immune cells in the polytrauma group, both locally at the fracture site and systemically, compared to other groups. The percentage of B and T cells was dramatically reduced in the polytrauma group 6 h after injury and remained low throughout the study duration. Fracture healing in the polytrauma group was impaired, evidenced by the formation of a poorly mineralized and dysregulated fracture callus. Our data confirm the early, dysregulated inflammatory state in polytrauma that correlates with disorganized and impaired fracture healing.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inducible displacement of cementless total knee arthroplasty components with conventional and weight-bearing CT-based radiostereometric analysis. 通过传统和负重 CT 放射立体测量分析无骨水泥全膝关节置换术组件的诱发位移。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-11-15 DOI: 10.1002/jor.26017

Rebecca A Hext, Bart L Kaptein, James L Howard, Brent A Lanting, Matthew G Teeter

{"title":"Inducible displacement of cementless total knee arthroplasty components with conventional and weight-bearing CT-based radiostereometric analysis.","authors":"Rebecca A Hext, Bart L Kaptein, James L Howard, Brent A Lanting, Matthew G Teeter","doi":"10.1002/jor.26017","DOIUrl":"https://doi.org/10.1002/jor.26017","url":null,"abstract":"Aseptic loosening remains one of the top causes of revision surgery of total knee arthroplasty (TKA). Radiostereometric analysis (RSA) is used in research to measure implant migration, however limitations prevent its clinical use. New methods have allowed the same measurements as RSA to be performed with computed tomography (CT) scanners (CT-RSA). The objective of this study is to determine inducible displacement measurements from weight-bearing computed tomography (WBCT) and conventional RSA to assess implant stability. Participants (n = 17) completed RSA exams in the supine and standing position, and WBCT exams in the seated (leg extended) and standing position. Double examinations were performed in the seated (WBCT) or supine (RSA) positions. Inducible displacements were measured with model-based RSA (MBRSA) for RSA exams, and a novel CT-RSA software, V3MA, for WBCT exams. Precision of each technique was calculated between double examinations. Precision data for tibial component total translations and rotations were 0.05 mm and 0.118°, respectively with WBCT-RSA, and were 0.108 mm and 0.269°, respectively with MBRSA. MTPM precision was 0.141 mm with WBCT-RSA and was 0.168 mm with MBRSA. Inducible displacement MTPM of the tibial component was 0.244 ± 0.220 mm with WBCT-RSA and 0.662 ± 0.257 mm with MBRSA. Inducible displacement measurements with MBRSA were significantly different from WBCT-RSA for tibial component anterior tilt (p = 0.0002). WBCT-RSA demonstrated comparable precision to MBRSA, and both techniques measured inducible displacements consistent with stable components. Clinical Significance: As the availability of WBCT increases, its use as an alternative to MBRSA is supported to measure the instantaneous fixation of implant components.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clearing-enabled light sheet microscopy as a novel method for three-dimensional mapping of the sensory innervation of the mouse knee. 透明光片显微镜是绘制小鼠膝关节感觉神经支配三维图的一种新方法。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-11-15 DOI: 10.1002/jor.26016

Frank C Ko, Spencer Fullam, Hoomin Lee, Kelly Chan, Shingo Ishihara, Natalie S Adamczyk, Alia M Obeidat, Sarah Soorya, Richard J Miller, Anne-Marie Malfait, Rachel E Miller

{"title":"Clearing-enabled light sheet microscopy as a novel method for three-dimensional mapping of the sensory innervation of the mouse knee.","authors":"Frank C Ko, Spencer Fullam, Hoomin Lee, Kelly Chan, Shingo Ishihara, Natalie S Adamczyk, Alia M Obeidat, Sarah Soorya, Richard J Miller, Anne-Marie Malfait, Rachel E Miller","doi":"10.1002/jor.26016","DOIUrl":"10.1002/jor.26016","url":null,"abstract":"A major barrier that hampers our understanding of the precise anatomic distribution of pain sensing nerves in and around the joint is the limited view obtained from traditional two dimensional (D) histological approaches. Therefore, our objective was to develop a workflow that allows examination of the innervation of the intact mouse knee joint in 3D by employing clearing-enabled light sheet microscopy. We first surveyed existing clearing protocols (SUMIC, PEGASOS, and DISCO) to determine their ability to clear the whole mouse knee joint, and discovered that a DISCO protocol provided the optimal transparency for light sheet microscopy imaging. We then modified the DISCO protocol to enhance binding and penetration of antibodies used for labeling nerves. Using the pan-neuronal PGP9.5 antibody, our protocol allowed 3D visualization of innervation in and around the mouse knee joint. We then implemented the workflow in mice intra-articularly injected with nerve growth factor (NGF) to determine whether changes in the nerve density can be observed. Both 3D and 2D analytical approaches of the light sheet microscopy images demonstrated quantifiable changes in midjoint nerve density following 4 weeks of NGF injection in the medial but not in the lateral joint compartment. We provide, for the first time, a comprehensive workflow that allows detailed and quantifiable examination of mouse knee joint innervation in 3D.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information - Editorial Board and TOC 期刊信息 - 编辑委员会和目录

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-11-12 DOI: 10.1002/jor.25622

引用次数: 0