Journal of Orthopaedic Research®最新文献_第2页

How accurately do finite element models predict the fall impact response of ex vivo specimens augmented by prophylactic intramedullary nailing? 有限元模型如何准确预测通过预防性髓内钉加固的体外标本的跌落冲击响应？

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-10-01 DOI: 10.1002/jor.25984

Emily K Bliven, Anita Fung, Alexander Baker, Ingmar Fleps, Stephen J Ferguson, Pierre Guy, Benedikt Helgason, Peter A Cripton

{"title":"How accurately do finite element models predict the fall impact response of ex vivo specimens augmented by prophylactic intramedullary nailing?","authors":"Emily K Bliven, Anita Fung, Alexander Baker, Ingmar Fleps, Stephen J Ferguson, Pierre Guy, Benedikt Helgason, Peter A Cripton","doi":"10.1002/jor.25984","DOIUrl":"https://doi.org/10.1002/jor.25984","url":null,"abstract":"Hip fracture prevention approaches like prophylactic augmentation devices have been proposed to strengthen the femur and prevent hip fracture in a fall scenario. The aim of this study was to validate the finite element model (FEM) of specimens augmented by prophylactic intramedullary nailing in a simulated sideways fall impact against ex vivo experimental data. A dynamic inertia-driven sideways fall simulator was used to test six cadaveric specimens (3 females, 3 males, age 63-83 years) prophylactically implanted with an intramedullary nailing system used to augment the femur. Impact force measurements, pelvic deformation, effective pelvic stiffness, and fracture outcomes were compared between the ex vivo experiments and the FEMs. The FEMs over-predicted the effective pelvic stiffness for most specimens and showed variability in terms of under- and over-predicting peak impact force and pelvis compression depending on the specimen. A significant correlation was found for time to peak impact force when comparing ex vivo and FEM data. No femoral fractures were found in the ex vivo experiments, but two specimens sustained pelvic fractures. These two pelvis fractures were correctly identified by the FEMs, but the FEMs made three additional false-positive fracture identifications. These validation results highlight current limitations of these sideways fall impact models specific to the inclusion of an orthopaedic implant. These FEMs present a conservative strategy for fracture prediction in future applications. Further evaluation of the modelling approaches used for the bone-implant interface is recommended for modelling augmented specimens, alongside the importance of maintaining well-controlled experimental conditions.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacological antagonism of Ccr2+ cell recruitment to facilitate regenerative tendon healing. 药理拮抗 Ccr2+ 细胞招募，促进肌腱再生愈合。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-10-01 DOI: 10.1002/jor.25986

Gilbert Smolyak, Andrew Rodenhouse, Anne E C Nichols, Constantinos Ketonis, Alayna E Loiselle

{"title":"Pharmacological antagonism of Ccr2+ cell recruitment to facilitate regenerative tendon healing.","authors":"Gilbert Smolyak, Andrew Rodenhouse, Anne E C Nichols, Constantinos Ketonis, Alayna E Loiselle","doi":"10.1002/jor.25986","DOIUrl":"10.1002/jor.25986","url":null,"abstract":"Successful tendon healing requires sufficient deposition and remodeling of new extracellular matrix at the site of injury, with this process mediating in part through fibroblast activation via communication with macrophages. Moreover, resolution of healing requires clearance or reversion of activated cells, with chronic interactions with persistent macrophages impairing resolution and facilitating the conversion to fibrotic healing. As such, modulation of the macrophage environment represents an important translational target to improve the tendon healing process. Circulating monocytes are recruited to sites of tissue injury, including the tendon, via upregulation of cytokines including Ccl2, which facilitates recruitment of Ccr2+ macrophages to the healing tendon. Our prior work has demonstrated that Ccr2-/- can modulate fibroblast activation and myofibroblast differentiation. However, this approach lacked temporal control and resulted in healing impairments. Thus, in the current study we have leveraged a Ccr2 antagonist to blunt macrophage recruitment to the healing tendon in a time-dependent manner. We first tested the effects of Ccr2 antagonism during the acute inflammatory phase and found that this had no effect on the healing process. In contrast, Ccr2 antagonism during the early proliferative/granulation tissue period resulted in significant improvements in mechanical properties of the healing tendon. Collectively, these data demonstrate the temporally distinct impacts of modulating Ccr2+ cell recruitment and Ccr2 antagonism during tendon healing and highlight the translational potential of transient Ccr2 antagonism to improve the tendon healing process.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of conventional and CT-based radiostereometric analysis for inducible displacement measurements after total hip arthroplasty. 评估全髋关节置换术后用于诱发位移测量的传统和基于 CT 的放射立体测量分析。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-10-01 DOI: 10.1002/jor.25981

Jennifer S Polus, Bart L Kaptein, Edward M Vasarhelyi, Brent A Lanting, Matthew G Teeter

{"title":"Evaluation of conventional and CT-based radiostereometric analysis for inducible displacement measurements after total hip arthroplasty.","authors":"Jennifer S Polus, Bart L Kaptein, Edward M Vasarhelyi, Brent A Lanting, Matthew G Teeter","doi":"10.1002/jor.25981","DOIUrl":"https://doi.org/10.1002/jor.25981","url":null,"abstract":"Though radiostereometric analysis (RSA) is the gold standard for migration tracking, computed tomography-based RSA (CT-RSA) does not require marker beads and is available for clinical adoption. This study investigated CT-RSA in comparison to RSA for assessing hip implant stability with inducible displacement (ID) examinations. Patients (n = 48) from a previous study returned to be re-examined for femoral stem stability with CT-RSA and RSA imaging. Implant migration since patients last follow-up was calculated as a measure of stability. ID was assessed between alternated leg rotation scans for CT-RSA and between supine and weight-bearing scans for RSA. Measurements from ID and double examinations were compared between CT-RSA and RSA. All stems were well-fixed with migration <0.2 mm/year. ID measurements were lower with CT-RSA than RSA for distal translation (mean difference = 0.122 mm, p < 0.0001), total translation (mean difference = 0.158 mm, p < 0.0001), and total rotation (mean difference = 0.449°, p < 0.0001). The ID and double exam were significantly different for total translation and total rotation for CT-RSA, and significantly different for medial, distal, and total translation, and total rotation for RSA. Precision ranged from 0.049 to 0.130 mm in translation and 0.061° to 0.220° in rotation for CT-RSA, and from 0.108 to 0.269 mm in translation and 0.151° to 0.670° in rotation for RSA. ID measurements from both CT-RSA and RSA were minimal, consistent for a cohort with well-fixed stems. CT-RSA demonstrated superior precision in all axes compared to RSA. Clinical Significance: Future work should explore the use of CT-RSA in patients with suspected loosening as a potential diagnostic tool.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cross-sectional morphology of the proximal femoral diaphysis is defined by the anteversion angle. 股骨近端骨骺的横截面形态由前倾角决定。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-30 DOI: 10.1002/jor.25983

Daisuke Endo, Keita Nishi, Takeshi Imamura, Kazunobu Saiki, Keiko Ogami-Takamura, Kiyohito Murai, Toshiyuki Tsurumoto

{"title":"The cross-sectional morphology of the proximal femoral diaphysis is defined by the anteversion angle.","authors":"Daisuke Endo, Keita Nishi, Takeshi Imamura, Kazunobu Saiki, Keiko Ogami-Takamura, Kiyohito Murai, Toshiyuki Tsurumoto","doi":"10.1002/jor.25983","DOIUrl":"https://doi.org/10.1002/jor.25983","url":null,"abstract":"Osteoporosis in postmenopausal women is one of the causes of femoral fractures and is prevented by the administration of bisphosphonates. Individual morphologies are considered to increase the risk of atypical fractures associated with long-term administration. To evaluate cortical bone morphology quantitatively, we established a method to measure the distance from the center point of a cross-section to the external and internal borders based on CT images. Using this method, 44 sides of a female femoral skeleton specimen were examined and areas of protrusion and thickening in the medial anterior and lateral posterior regions just below the lesser trochanter were identified. These positions strongly correlated with the anteversion angle, suggesting the involvement of the distribution of the load received from body weight defined by the angle. The finite element method was used to examine the relationships between the positions of these areas with compressive and tensile stress distribution areas in the one-legged standing condition. The medial anterior region and lateral posterior region protruded and thickened in response to compressive and tensile stress, respectively. In addition, a hierarchical relationship was observed between the anteversion angle, tensile stress distribution, protrusion, and thickening in femurs with thinning of cortical bone, indicating that morphogenesis occurs adaptively to loading. The present results demonstrate the usefulness of this method in considering the formation mechanism and function of the femoral diaphysis and suggest that bone remodeling is necessary to maintain adaptability.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cervical motion analysis using wearable inertial sensors to patients with cervical ossification of posterior longitudinal ligament. 使用可穿戴惯性传感器对颈椎后纵韧带骨化患者进行颈椎运动分析。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-29 DOI: 10.1002/jor.25985

Satoshi Osuka, Masahiko Takahata, Ryo Takeda, Takeshi Chiba, Hiroaki Hori, Yoshiaki Kataoka, Norimasa Iwasaki, Masahiko Mukaino, Harukazu Tohyama

{"title":"Cervical motion analysis using wearable inertial sensors to patients with cervical ossification of posterior longitudinal ligament.","authors":"Satoshi Osuka, Masahiko Takahata, Ryo Takeda, Takeshi Chiba, Hiroaki Hori, Yoshiaki Kataoka, Norimasa Iwasaki, Masahiko Mukaino, Harukazu Tohyama","doi":"10.1002/jor.25985","DOIUrl":"https://doi.org/10.1002/jor.25985","url":null,"abstract":"This study aimed to clarify cervical kinematics during daily activities, including level walking and stair ascending, in patients with cervical ossification of the posterior longitudinal ligament (C-OPLL). Eighteen patients with myelopathy caused by C-OPLL and 18 healthy controls were recruited to participate in the study. The sagittal cervical kinematics during level walking and stair ascent were quantitatively assessed using a motion analysis system based on wearable inertial sensors. The Japanese Orthopaedic Association score, Japanese Orthopaedic Association Cervical Myelopathy Evaluation Questionnaire, Neck Disability Index, and deep sensation in the lower extremities were assessed in all participants. Nine of 18 patients with C-OPLL presented with deep sensory disturbances. Patients with C-OPLL with deep sensory disturbances exhibited different sagittal plane cervical motion patterns than healthy controls during level walking and stair ascent. During the first phase of stair ascent, both patients with C-OPLL and healthy controls flexed their necks to the same degree; however, during the middle and final phases of stair ascent and all phases of level walking, the mean cervical flexion angle of patients with C-OPLL with deep sensory disturbances was significantly higher than that of patients with C-OPLL without deep sensory disturbance and healthy controls. Our data suggest that patients with C-OPLL presenting with deep sensory disturbances are likely to walk with their necks flexed and gaze downward to observe their steps throughout their daily lives. This habitual neck posture may lead to a vicious cycle of cervical kyphosis and worsening of compressive myelopathy.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal high-fat high-sugar diet impairs bone quality and strength but not cartilage in aging mice. 母体高脂高糖饮食会损害衰老小鼠的骨骼质量和强度，但不会损害软骨。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-29 DOI: 10.1002/jor.25980

Arin K Oestreich, Natalia S Harasymowicz, Alireza Savadipour, Zainab Harissa, Neda Rashidi, Meredith K Luhmann, Mohammed Kuziez, Kelle H Moley, Farshid Guilak

{"title":"Maternal high-fat high-sugar diet impairs bone quality and strength but not cartilage in aging mice.","authors":"Arin K Oestreich, Natalia S Harasymowicz, Alireza Savadipour, Zainab Harissa, Neda Rashidi, Meredith K Luhmann, Mohammed Kuziez, Kelle H Moley, Farshid Guilak","doi":"10.1002/jor.25980","DOIUrl":"https://doi.org/10.1002/jor.25980","url":null,"abstract":"Osteoarthritis (OA) is a prevalent aging disorder of synovial joints and recent work suggests that a parental high-fat diet increases OA severity following joint injury in offspring. We hypothesized that a maternal high-fat high-sugar (HFHS) diet would promote spontaneous osteoarthritis-related cartilage and bone changes in 1-year-old offspring. Female C57BL/6 J mice were placed on either a chow control or HFHS diet for 6 weeks before mating to a chow-fed C57BL/6 J male and maintained on their assigned diets throughout pregnancy and lactation. Male and female offspring were weaned onto a chow diet, raised to 1 year of age, and evaluated for cartilage and bone changes indicative of OA. However, offspring did not show early signs of OA as measured by histological Mankin scoring, mechanical testing of the pericellular matrix, histological synovitis scoring, or subchondral bone thickening as measured by microcomputed Tomography. On the other hand, male offspring from HFHS-fed dams had reduced trabecular bone quality in the tibial metaphysis and decreased cortical thickness. Although maternal HFHS diet did not impact trabecular or cortical bone quality in tibias of female offspring, the radii of these animals had decreased cortical thickness, increased medullary area, and impaired breaking strength compared to those of control-fed dams. Finally, we evaluated bone quality and strength in male and female F2 offspring and found that the grandmaternal diet modestly impacted radial bone geometry but not strength. Together these results suggest that maternal HFHS diet impairs F1 offspring skeletal integrity in a sex and bone site-specific manner.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell migration within porous electrospun nanofibrous scaffolds in a mouse subcuticular implantation model. 小鼠皮下植入模型中多孔电纺纳米纤维支架内的细胞迁移。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-29 DOI: 10.1002/jor.25979

Waleed Mohammad, Liang Chen, Bin Wu, Paula Dietz, Therese Bou-Akl, Weiping Ren, David C Markel

{"title":"Cell migration within porous electrospun nanofibrous scaffolds in a mouse subcuticular implantation model.","authors":"Waleed Mohammad, Liang Chen, Bin Wu, Paula Dietz, Therese Bou-Akl, Weiping Ren, David C Markel","doi":"10.1002/jor.25979","DOIUrl":"https://doi.org/10.1002/jor.25979","url":null,"abstract":"Cellular infiltration into electrospun nanofibers (NFs) is limited due to the dense structure and small pore sizes. We developed a programmed NF collector that can fabricate porous NFs with desired pore sizes and thickness. Previously we demonstrated improved cellular proliferation and differentiation of osteoblasts, osteoclasts, and fibroblasts with increased pore sizes of polycaprolactone (PCL) NF in-vitro. This study investigated in-vivo host cell migration and vascular ingrowth within porous NF sheets implanted subcutaneously in a mouse model. Two types of PCL NFs with well-defined pore sizes were created using varying speeds of the NF collector: NF-zero (no movement, pore size 14.4 ± 8.9 µm2) and NF-high (0.232 mm/min, pore size 286.7 ± 381.9 µm2). The NF obtained by using classical flat NF collector (2D NF, pore size 1.09 ± 1.7 µm2) was a control. The three formulae of NFs were implanted subcutaneously in 18 BALB/cJ mice. Animals were killed 7 and 28-days after implantation (n = 3 per group per time point). The tissue with implanted NFs were collected for histologic analysis. Overall, 7-day samples showed little inflammatory response. At 28-days, the degree of tissue penetration of PCL NF sheet matrices was linked to pore size and area. NFs with the largest pore area had more efficient tissue migration and new blood vessel formation compared to those with smaller pore sizes. No newly formed blood vessels were observed in the 2D NF group. A porous NF scaffold with controllable pore size has potential for tissue repair/regeneration in situ with potential for many applications in orthopaedics.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An in vitro study of glenoid implant peripheral peg interface mechanics during eccentric loading and lift-off. 偏心加载和抬起时髋臼假体外周钉界面力学的体外研究。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-25 DOI: 10.1002/jor.25976

Jakub Targosinski, Jonathan Kusins, George Athwal, Andrew Nelson, Louis Ferreira

{"title":"An in vitro study of glenoid implant peripheral peg interface mechanics during eccentric loading and lift-off.","authors":"Jakub Targosinski, Jonathan Kusins, George Athwal, Andrew Nelson, Louis Ferreira","doi":"10.1002/jor.25976","DOIUrl":"https://doi.org/10.1002/jor.25976","url":null,"abstract":"Glenoid implants used in anatomic total shoulder arthroplasties typically incorporate peripheral pegs as a design feature to support eccentric loads. These peripheral pegs and the implant-cement-bone interface undergo substantial cyclic tensile-compressive loads during normal activity. Therefore, these pegs are of interest in translating the micromechanics of local implant fixation failure to the biomechanics of gross anatomic failure of the glenoid implant after total shoulder arthroplasty. This study used an in vitro peg-cement-bone construct which incorporated bone tissue acquired from osteoarthritic patients undergoing total shoulder arthroplasty. Strain distributions in the peripheral peg-cement-bone interfaces were analyzed under loading conditions emulating glenoid implant edge displacements. It was found that tensile strains in the interfaces were highest near the backside-peg junction and were greater in magnitude than compressive strains. Notably, strains near the peg's fixation channels were relatively low. These results suggest that cracks may initiate around the peg near the backside and travel downward to cause broader fixation failure.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of CGRP receptor attenuates tendon graft degeneration in anterior cruciate ligament reconstruction. CGRP 受体的过度表达可减轻前十字韧带重建中肌腱移植物的退化。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-25 DOI: 10.1002/jor.25978

Xibang Zhao, Zhaoji Cai, Ying Luo, Zhousheng Lin, Jiali Wang

{"title":"Overexpression of CGRP receptor attenuates tendon graft degeneration in anterior cruciate ligament reconstruction.","authors":"Xibang Zhao, Zhaoji Cai, Ying Luo, Zhousheng Lin, Jiali Wang","doi":"10.1002/jor.25978","DOIUrl":"https://doi.org/10.1002/jor.25978","url":null,"abstract":"Cell apoptosis or necrosis, extracellular matrix loss, and excessive inflammation may induce tendon graft degeneration. The impairment in the regeneration capability of nerve fibers and blood vessels may be the critical cause. Calcitonin gene-related peptide (CGRP), exhibiting a short half-life, favors cell proliferation, nerve fiber regeneration and angiogenesis. We aimed to investigate the effects of CGRP receptor-mediated signaling on tendon graft integrity and study if the modulation pathways are ascribed to cell proliferation, nerve fiber and blood vessel regeneration. A total of three groups in mice with ACL reconstruction were established: the control group (PBS treatment), the adenovirus vectors expressing CGRP receptor (CALCRL) treated group (Adv-Calcrl treatment), and the adenovirus vectors carrying shRNA targeting Calcrl treated group (Adv-shCalcrl treatment). The histological assessment indicated the Adv-Calcrl treatment was favored while the Adv-shCalcrl significantly impaired tendon graft integrity. TUNEL staining revealed a significant decreased number of apoptotic cells in the Adv-Calcrl group relative to the control group and the adv-shCalcrl group. Compared to the control group and the Adv-shCalcrl group, the Adv-Calcrl group showed significantly enhanced proliferation of nestin positive cells. Of note, the Adv-Calcrl treatment significantly increased EMCN expression at the tendon graft relative to the control and the Adv-shCalcrl groups, which may be ascribed to attenuation of the Hippo signaling pathway. Importantly, the Adv-Calcrl treatment significantly increased sensory nerve fibers and also PIEZO2 levels. Our results demonstrate the activation of CGRP receptor-mediated signaling attenuated tendon graft degeneration, which was ascribed to enhanced proliferation of Nestin positive cells, angiogenesis, and nerve fiber outgrowth.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of collagen content is localized near cartilage lesions on the day of injurious loading and intensified on day 12. 胶原蛋白含量的损失主要集中在软骨损伤当天的损伤部位，并在第 12 天加剧。

IF 2.1 3区医学

Journal of Orthopaedic Research® Pub Date : 2024-09-23 DOI: 10.1002/jor.25975

Moustafa Hamada, Atte S A Eskelinen, Cristina Florea, Santtu Mikkonen, Petteri Nieminen, Alan J Grodzinsky, Petri Tanska, Rami K Korhonen

{"title":"Loss of collagen content is localized near cartilage lesions on the day of injurious loading and intensified on day 12.","authors":"Moustafa Hamada, Atte S A Eskelinen, Cristina Florea, Santtu Mikkonen, Petteri Nieminen, Alan J Grodzinsky, Petri Tanska, Rami K Korhonen","doi":"10.1002/jor.25975","DOIUrl":"https://doi.org/10.1002/jor.25975","url":null,"abstract":"Joint injury can lead to articular cartilage damage, excessive inflammation, and post-traumatic osteoarthritis (PTOA). Collagen is an essential component for cartilage function, yet current literature has limited understanding of how biochemical and biomechanical factors contribute to collagen loss in injured cartilage. Our aim was to investigate spatially dependent changes in collagen content and collagen integrity of injured cartilage, with an explant model of early-stage PTOA. We subjected calf knee cartilage explants to combinations of injurious loading (INJ), interleukin-1α-challenge (IL) and physiological cyclic loading (CL). Using Fourier transform infrared microspectroscopy, collagen content (Amide I band) and collagen integrity (Amide II/1338 cm-1 ratio) were estimated on days 0 and 12 post-injury. We found that INJ led to lower collagen content near lesions compared to intact regions on day 0 (p < 0.001). On day 12, near-lesion collagen content was lower compared to day 0 (p < 0.05). Additionally, on day 12, INJ, IL, and INJ + IL groups exhibited lower collagen content along most of tissue depth compared to free-swelling control group (p < 0.05). CL groups showed higher collagen content along most of tissue depth compared to corresponding groups without CL (p < 0.05). Immunohistochemical analysis revealed higher MMP-1 and MMP-3 staining intensities localized within cell lacunae in INJ group compared to CTRL group on day 0. Our results suggest that INJ causes rapid loss of collagen content near lesions, which is intensified on day 12. Additionally, CL could mitigate the loss of collagen content at intact regions after 12 days.","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0