Journal of nutritional science and vitaminology最新文献_第9页

VDR Is a Novel Regulator of Cellular Senescence in Lens Epithelial Cells. VDR是一种新的晶状体上皮细胞衰老调节剂。

IF 1.1 4区医学

Journal of nutritional science and vitaminology Pub Date : 2025-01-01 DOI: 10.3177/jnsv.71.395

Tomofumi Yatsu, Momoka Koyama, Yoshiki Miyata

{"title":"VDR Is a Novel Regulator of Cellular Senescence in Lens Epithelial Cells.","authors":"Tomofumi Yatsu, Momoka Koyama, Yoshiki Miyata","doi":"10.3177/jnsv.71.395","DOIUrl":"https://doi.org/10.3177/jnsv.71.395","url":null,"abstract":"Age-related cataracts (ARC) are a leading cause of blindness worldwide, with lens epithelial cell (LEC) senescence playing a key role in their progression. While oxidative stress and DNA damage are known to induce LEC senescence, the mechanisms that actively suppress or delay this process remain poorly understood. The vitamin D receptor (VDR), a nuclear receptor and transcription factor, has been implicated in various physiological processes, including calcium homeostasis, immune modulation, and cell proliferation. Additionally, VDR activation has been reported to protect against cellular aging and oxidative stress. However, the role of VDR in LEC senescence and its potential function in preventing ARC has not been fully elucidated. In this study, we investigated the role of VDR in regulating LEC senescence using the human lens epithelial cell line SRA01/04. VDR knockdown induced senescence markers, including increased SA-β-GAL activity, p21 expression, and γ-H2AX accumulation and reduced Lamin B1 expression. Additionally, VDR knockdown led to a significant increase in intracellular reactive oxygen species (ROS) levels. Conversely, VDR overexpression suppressed H2O2-induced senescence and enhanced the expression of antioxidant enzymes such as SOD2 and GPX1. These findings indicate that VDR modulates LEC senescence by regulating oxidative stress and antioxidant enzyme expression, suggesting that VDR may be a potential therapeutic target for ARC prevention. Further studies, particularly in vivo models, are necessary to validate these findings and explore the translational potential of targeting VDR in cataract therapy.","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 5","pages":"395-401"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145438274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Asparagus-Derived 20-Hydroxyecdysone Supplementation on Fat Oxidation and Insulin Sensitivity in Resistance-Trained Males. 补充芦笋衍生的20-羟基蜕皮激素对抵抗训练男性脂肪氧化和胰岛素敏感性的影响。

IF 1.1 4区医学

Journal of nutritional science and vitaminology Pub Date : 2025-01-01 DOI: 10.3177/jnsv.71.526

Siriporn Sripinyowanich, Barakat Denben, Pimchanok Satapoomin, Piyaporn Tumnark, Jatuporn Phoemsapthawee

{"title":"Effects of Asparagus-Derived 20-Hydroxyecdysone Supplementation on Fat Oxidation and Insulin Sensitivity in Resistance-Trained Males.","authors":"Siriporn Sripinyowanich, Barakat Denben, Pimchanok Satapoomin, Piyaporn Tumnark, Jatuporn Phoemsapthawee","doi":"10.3177/jnsv.71.526","DOIUrl":"10.3177/jnsv.71.526","url":null,"abstract":"This study investigated the effects of 20-hydroxyecdysone (20E) derived from Asparagus officinalis extract on substrate utilization and metabolic health in young males undergoing resistance training (RT). Twenty healthy males were randomly assigned in a double-blind design to receive either a placebo (PLA; n=10) or 30 mg/d of 20E (n=10) for 12 wk, during which all participants performed supervised RT three times per week. Assessments were conducted at baseline and post-intervention, including body composition, substrate utilization, fasting glucose, insulin, free fatty acids (FFAs), and the quantitative insulin sensitivity check index (QUICKI). The 20E group showed significant reductions in arm (p<0.01), leg (p<0.05), and abdominal fat (p<0.05), with arm fat reduction significantly greater than in the PLA group (p<0.05). Fat oxidation increased at rest and during exercise in the 20E group (p<0.01), with between-group differences evident only at 40% of V•O2peak (p<0.05). Furthermore, 20E supplementation was associated with favorable metabolic changes, including reductions in fasting insulin (p<0.01) and plasma FFAs (p<0.05), alongside an increase in QUICKI (p<0.01). These findings suggest that 20E supplementation, when combined with RT, may support regional fat reduction, promote fat oxidation, and improve insulin sensitivity, thereby offering potential benefits for metabolic health.","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 6","pages":"526-536"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validity of Digital Photographic Images for Dietary Assessment of Participants with Low Frequency of Home-Made Meal Intake. 数字摄影图像对低频率家庭膳食摄入参与者膳食评估的有效性。

IF 1.1 4区医学

Journal of nutritional science and vitaminology Pub Date : 2025-01-01 DOI: 10.3177/jnsv.71.349

Tomomi Nagahata, Maki Igarashi, Yuko Mekata, Yuki Kokubo, Naoki Takayanagi, Noriko Suzuki-Kemuriyama, Haruhide Udagawa, Sosuke Amano, Kiyoharu Aizawa, Yoko Yamakata, Hisanori Kato

{"title":"Validity of Digital Photographic Images for Dietary Assessment of Participants with Low Frequency of Home-Made Meal Intake.","authors":"Tomomi Nagahata, Maki Igarashi, Yuko Mekata, Yuki Kokubo, Naoki Takayanagi, Noriko Suzuki-Kemuriyama, Haruhide Udagawa, Sosuke Amano, Kiyoharu Aizawa, Yoko Yamakata, Hisanori Kato","doi":"10.3177/jnsv.71.349","DOIUrl":"https://doi.org/10.3177/jnsv.71.349","url":null,"abstract":"It is important to clarify the factors that cause errors when calculating nutrient and food group intake using dietary surveys. This study aimed to clarify the characteristics of errors in nutrient and food group intake among those who ate out and those who consumed ready-made meals when they recorded their meals using photographic and weighing methods. In October 2020, 38 second- and third-year students who were enrolled in dietitian training programs at two Japanese universities were asked to record their meals using both photographic and weighed records. Nutrient calculations were performed from the dietary records of both methods, and data from 29 subjects (all female) for whom 4-d dietary records were obtained were analyzed. The subjects were divided into two groups (high and low frequency of home-made meals) based on the median number of home-made meals, and a correlation analysis of the nutritional calculations obtained using both methods was performed. The results showed that most nutrient and food group intake levels were correlated (Spearman's correlation coefficient r=0.6-0.9) for both the high and low frequency of home-made meals. However, no correlation was found for \"seasonings and spices\" or their component \"salt\" in the low frequency of home-made meals group, indicating that there are errors in the nutritional calculations, especially for salt. It is thought that errors occurred for both the recorder and the nutrition calculator during the survey of meals eaten out; therefore, it is necessary to consider how to minimize errors before dietary surveys.","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 4","pages":"349-356"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum 25-Hydroxyvitamin D Levels and the Risk of Post-Traumatic Wound Infection. 血清25-羟基维生素D水平与创伤后伤口感染的风险。

IF 0.7 4区医学

Journal of nutritional science and vitaminology Pub Date : 2025-01-01 DOI: 10.3177/jnsv.71.110

Zhanyi Zhang, Xizhe Yang, Mengyuan Li, Bo Li

{"title":"Serum 25-Hydroxyvitamin D Levels and the Risk of Post-Traumatic Wound Infection.","authors":"Zhanyi Zhang, Xizhe Yang, Mengyuan Li, Bo Li","doi":"10.3177/jnsv.71.110","DOIUrl":"https://doi.org/10.3177/jnsv.71.110","url":null,"abstract":"Post-traumatic wound infection is commonly observed in trauma patients and has a poor prognosis. Vitamin D is a significant modulator of the immune system and may play a role in preventing wound infection. However, the inherent limitations and inconsistent results of previous observational studies restricted them from inferring causal effects. We employed Mendelian randomization design to estimate the causal effects of serum 25-hydroxyvitamin D levels on the risk of post-traumatic wound infection. Data in the analysis were extracted from large-scale genome-wide association studies with 417,580 individuals for serum 25-hydroxyvitamin D measurements and 218,546 individuals for post-traumatic wound infection. Multivariable Mendelian randomization analyses were performed to adjust for confounding variables. We also conducted extensive sensitivity analyses to verify the main results. The results revealed a causal relationship between higher serum 25-hydroxyvitamin D levels and a lower post-traumatic wound infection risk (OR=0.473, p=0.014). The causality remained after adjustment of potential confounding factors according to Multivariable Mendelian randomization analyses (OR=0.361, p<0.001). Sensitivity tests suggested the reliability of our findings. This study indicated a causal association between genetically predicted high serum 25-hydroxyvitamin D levels and a decreased risk of post-traumatic wound infection.","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 2","pages":"110-117"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thanks to Reviewers. 感谢评论者。

IF 0.7 4区医学

Journal of nutritional science and vitaminology Pub Date : 2025-01-01 DOI: 10.3177/jnsv.71.184

引用次数: 0

Glucose and Insulin Differently Regulate Gluconeogenic and Ureagenic Gene Expression. 葡萄糖和胰岛素对糖异生和尿原基因表达的调控不同。

IF 0.7 4区医学

Journal of nutritional science and vitaminology Pub Date : 2025-01-01 DOI: 10.3177/jnsv.71.46

Kanako Deguchi, Chihiro Ushiroda, Yuka Kamei, Kyosuke Kondo, Hiromi Tsuchida, Yusuke Seino, Daisuke Yabe, Atsushi Suzuki, Shizuko Nagao, Katsumi Iizuka

{"title":"Glucose and Insulin Differently Regulate Gluconeogenic and Ureagenic Gene Expression.","authors":"Kanako Deguchi, Chihiro Ushiroda, Yuka Kamei, Kyosuke Kondo, Hiromi Tsuchida, Yusuke Seino, Daisuke Yabe, Atsushi Suzuki, Shizuko Nagao, Katsumi Iizuka","doi":"10.3177/jnsv.71.46","DOIUrl":"10.3177/jnsv.71.46","url":null,"abstract":"Glucose and insulin positively regulate glycolysis and lipogenesis through the activation of carbohydrate response element-binding protein (ChREBP) and sterol regulatory element-binding protein 1c (SREBP1c), but their respective roles in the regulation of gluconeogenic and ureagenic genes remain unclear. We compared the effects of the insulin antagonist S961 and Chrebp deletion on hepatic glycolytic, lipogenic, gluconeogenic, and ureagenic gene expression in mice. S961 markedly increased the plasma glucose, insulin, and 3-OH-butyrate concentrations and reduced the hepatic triglyceride content, but Chrebp deletion had no additive effect. We subsequently estimated the expression of genes involved in the pathways of glycolysis, gluconeogenesis, and lipogenesis. S961 potently decreased both Chrebp and Srebf1c, but Chrebp deletion weakly decreased Srebf1c mRNA expression. Both the S961 and Chrebp deletion caused decreases in glycolytic (Gck and Pklr) and lipogenic (Fasn, Scd1, Me1, Spot14, Elovl6) gene expression. S961 increased the expression of many gluconeogenic genes (G6pc, Fbp1, Aldob, Slc37a4, Pck), whereas Chrebp deletion reduced the expression of gluconeogenic genes other than Pck1. Finally, we checked the metabolites and gene expression in the ureagenesis pathway. S961 increased ureagenic gene (Arg1, Asl, Ass1, Cps1, Otc) expression, which was consistent with the metabolite data: there were reductions in the concentrations of glutamate and aspartate and increases in those of citrulline, ornithine, urea, and proline. However, Chrebp deletion had no additive effect on ureagenesis. In conclusion, insulin rather than glucose regulate ureagenic gene expression, whereas glucose and insulin regulate gluconegenic gene expression in opposite directions.","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 1","pages":"46-54"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prenatal Exposure to Bonito Broth Attenuates the Induction of Drd1 and Grin1 upon Corn Oil Ingestion in Offspring. 产前接触鲣鱼肉汤可减弱后代摄入玉米油对Drd1和Grin1的诱导作用。

IF 1.1 4区医学

Journal of nutritional science and vitaminology Pub Date : 2025-01-01 DOI: 10.3177/jnsv.71.552

Shunsuke Fushimi, Takaaki Ozawa, Sho Matsui, Satoshi Tsuzuki, Takatoshi Hikida, Tsutomu Sasaki

{"title":"Prenatal Exposure to Bonito Broth Attenuates the Induction of Drd1 and Grin1 upon Corn Oil Ingestion in Offspring.","authors":"Shunsuke Fushimi, Takaaki Ozawa, Sho Matsui, Satoshi Tsuzuki, Takatoshi Hikida, Tsutomu Sasaki","doi":"10.3177/jnsv.71.552","DOIUrl":"https://doi.org/10.3177/jnsv.71.552","url":null,"abstract":"Nutritional environments in the early life shape the development of neural systems involved in rewards. While the effects of maternal nutritional status on offspring feeding behavior have been studied, the impact of non-nutrient dietary components remains poorly understood. We previously reported that prenatal exposure to bonito broth (katsuo-dashi) reduces the motivation of adult offspring to consume fat. In the present study, we explored the potential molecular mechanisms underlying this phenomenon. First, we confirmed that bonito broth intake during gestation did not affect maternal nutrition or body weight, ruling out maternal undernutrition as a confounding factor for the offspring phenotype. Using fiber photometry, we determined that corn oil intake increased dopamine release in the nucleus accumbens (NAc), but the response did not correlate with motivational behavior. In control offspring, the mRNA expression of genes related to dopamine (Drd1), opioid (Oprm1, Oprk1), glutamate (Grin1), and GABA (Gabra1) signaling was upregulated in the NAc after exposure to corn oil, and the response was significantly attenuated in the gestation group. These changes in gene expression were not observed in the ventral tegmental area or in the amygdala. These findings suggest that prenatal exposure to bonito broth through the mother attenuates transcriptional responses to dietary fat in reward-related brain regions, particularly the NAc, without affecting dopamine dynamics or maternal nutrition.","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 6","pages":"552-563"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Foods, Including Rice, Miso Soup, and Japanese Tea, on the Absorption of Zinc. 大米、味噌汤和日本茶等食物对锌吸收的影响。

IF 1.1 4区医学

Journal of nutritional science and vitaminology Pub Date : 2025-01-01 DOI: 10.3177/jnsv.71.544

Motomi Shibasaki, Hiroko Kodama, Yorika Matsuda, Ritsuna Noguchi, Haruka Ueno, Yusaku Nakao

{"title":"Effect of Foods, Including Rice, Miso Soup, and Japanese Tea, on the Absorption of Zinc.","authors":"Motomi Shibasaki, Hiroko Kodama, Yorika Matsuda, Ritsuna Noguchi, Haruka Ueno, Yusaku Nakao","doi":"10.3177/jnsv.71.544","DOIUrl":"10.3177/jnsv.71.544","url":null,"abstract":"Only approximately 30% of ingested zinc (Zn) is absorbed. Some foods consumed with Zn affect its intestinal absorption. Zn supplementation is generally recommended immediately after meals to minimize gastric irritation. In this study, we examined the effects of food intake on Zn absorption. Zn acetate (25 mg as zinc) was administered orally to 13 healthy young volunteers either in the fasting state or with foods, such as 150 g of brown rice, white rice, beef hamburg, 200 mL of miso soup (fermented soybean paste soup), seaweed soup, cow's milk, Japanese tea and orange juice (100% fruit juice). Blood samples were collected before and at 1, 2, 3, and 4 h after administration of Zn alone, food alone (without Zn), or food with Zn. Zn absorption was compared using the area under the curve. The results showed that serum Zn levels hardly increased when Zn was administered with brown rice, whereas the Zn level was not inhibited by coadministration with seaweed soup. The absorption of Zn acetate with other foods was reduced to 14-66% compared to that when Zn was administered alone. Our results suggest that brown rice, which is a popular food in Japan and other countries, is associated with decreased Zn absorption.","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 6","pages":"544-551"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between Circulating Vitamin D Concentration and the Maternal Gut Microbiota during Early Pregnancy in Japanese Women: Results of a Pilot Study of Antenatal Vitamin D₃ Supplementation. 日本妇女妊娠早期循环维生素D浓度与母体肠道微生物群的关系：产前补充维生素D3的初步研究结果

IF 0.7 4区医学

Journal of nutritional science and vitaminology Pub Date : 2025-01-01 DOI: 10.3177/jnsv.71.185

Hyun Sook Kang, Ichiro Yamade, Takuya Inoue, Masahiro Otsubo, Hironori Hamada, Shinsuke Sudo, Nozomi Ogiso, Sotaro Hamakita, Morio Sawada, Hiroshi Hatayama

{"title":"Relationship between Circulating Vitamin D Concentration and the Maternal Gut Microbiota during Early Pregnancy in Japanese Women: Results of a Pilot Study of Antenatal Vitamin D3 Supplementation.","authors":"Hyun Sook Kang, Ichiro Yamade, Takuya Inoue, Masahiro Otsubo, Hironori Hamada, Shinsuke Sudo, Nozomi Ogiso, Sotaro Hamakita, Morio Sawada, Hiroshi Hatayama","doi":"10.3177/jnsv.71.185","DOIUrl":"https://doi.org/10.3177/jnsv.71.185","url":null,"abstract":"Recent studies have demonstrated a significant role for gut microbiota dysbiosis in the pathogenesis of various diseases, pregnancy outcomes, and fetal growth. Vitamin D modulates innate immunity and promotes immune tolerance, suggesting involvement in shaping the gut microbiota. However, the relationship between vitamin D and the gut microbiota during pregnancy has not been evaluated in detail. In this study, we administered a vitamin D3 supplement (VD) to pregnant Japanese women, starting during the first trimester of pregnancy, and characterized changes in the circulating concentration of vitamin D and the gut microbiome. Sixty-two pregnant women were randomly allocated to three groups. VD was administered at low (200 IU/d), medium (400 IU/d), or high (1,000 IU/d) doses for 8 wk from 12 to 15 wk of pregnancy, and changes in the serum 25-hydroxyvitamin D (25(OH)D) concentration and the gut microbiome were evaluated. Changes in gut microbial taxa abundance were analyzed by Wilcoxon matched-pairs signed-rank test. Before VD administration, 96.8% of the participants were VD-deficient, and none had a sufficient VD concentration. The serum 25(OH)D concentration increased in a dose-dependent manner in all the dose groups. Additionally, the abundance of Fusicatenibacter in the gut microbiota increased in a VD dosedependent manner, with a significant increase observed in the high-dose group. From these results, in pregnant Japanese women, the administration of high-dose VD increases the prevalence of VD sufficiency and alters the gut microbial composition, suggesting that this has benefits for the maternal and neonatal immune systems.","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 3","pages":"185-191"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Administration of Antibiotics Downregulates the Expression of MicroRNA-200 Family Members in Lamina Propria T Cells of the Murine Large Intestine. 抗生素治疗下调小鼠大肠固有层T细胞MicroRNA-200家族成员的表达

IF 0.7 4区医学

Journal of nutritional science and vitaminology Pub Date : 2025-01-01 DOI: 10.3177/jnsv.71.272

Fumina Ohsaka, Daiki Honma, Kei Sonoyama

{"title":"Administration of Antibiotics Downregulates the Expression of MicroRNA-200 Family Members in Lamina Propria T Cells of the Murine Large Intestine.","authors":"Fumina Ohsaka, Daiki Honma, Kei Sonoyama","doi":"10.3177/jnsv.71.272","DOIUrl":"https://doi.org/10.3177/jnsv.71.272","url":null,"abstract":"By comparing germ-free mice and specific pathogen-free mice, we previously demonstrated that gut commensals upregulate the expression of microRNA-200 family members, i.e., miR-200a, miR-200b, miR-200c, miR-141, and miR-429, in lamina propria leukocytes (LPLs) of the murine large intestine, and that it results in decreased production of interleukin-2, which is mainly produced by activated CD4+ T cells. In the present study, we investigated whether depletion of the gut microbiota by antibiotic treatment reduces the expression of miR-200 family members in T cells of the large intestinal lamina propria in mice. Supplementation of drinking water with antibiotics for 1 wk reduced the number of cecal bacteria in male C57BL/6J mice. LPLs of the large intestine were isolated and separated into T-cell and non-T-cell fractions by magnetic-activated cell sorting. Reverse transcription-quantitative polymerase chain reaction results showed that the expression levels of miR-200 family members were higher in the T-cell fraction than in the non-T-cell fraction, and were lower in the T-cell fraction of the antibiotic-treated mice than in that of the control mice. These results suggest that antibiotic-sensitive gut commensals upregulate the expression of miR-200 family members primarily in the T cells of the large intestinal lamina propria in mice.","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 3","pages":"272-276"},"PeriodicalIF":0.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0