Journal of Oral Pathology & Medicine最新文献

{"title":"Oral Melanocytic Neoplasms: A Narrative Review","authors":"J. Maldonado-Mendoza","doi":"10.1111/jop.70094","DOIUrl":"10.1111/jop.70094","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Oral melanocytic neoplasms, such as oral melanocytic nevi (OMN), oral dysplastic nevi (ODN), atypical melanocytic proliferation (AMP), and oral mucosal melanoma (OMM), represent &lt; 1% of oral lesions. They are composed of pigment-producing cells with distinct biological behaviors. This study aimed to summarize the clinicopathological and molecular features of these lesions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Studies published in English (1950–2025) were retrieved from the PubMed database and Web of Science Core Collection. All articles presenting clinicopathological and molecular outcomes of OMN, ODN, AMP, and OMM were included. A narrative summary was created to describe the findings.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>\u0000 OMN and OMM are well-described from a clinicopathological perspective. However, ODN and AMP remain scarcely documented. The genetic landscape of OMN suggests a limited role for driver mutations in \u0000 <i>BRAF</i>\u0000 or \u0000 <i>GNAQ</i>\u0000 , which promote the proliferation of pigmented-producing cells, often insufficient for malignancy. OMM exhibits a distinct molecular profile characterized by a scarcity of MAPK mutations and numerous copy-number changes, as well as amplifications and chromosomal rearrangements. In contrast, no genetic data are available for ODN and AMP.\u0000 </p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Oral melanocytic neoplasms are rare and have distinct clinicopathological features. Despite this, a gap exists in molecular data regarding ODN and AMP. Conversely, OMN and OMM have distinct profiles; in particular, the latter may benefit modestly from tyrosine kinase inhibitor treatment, as \u0000 <i>KIT</i>\u0000 and \u0000 <i>BRAF</i>\u0000 mutations are sensitive to imatinib and vemurafenib, respectively.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 4","pages":"429-438"},"PeriodicalIF":2.3,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145742848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age, Sex, and Neuroinflammation: A Unified Mechanism for Burning Mouth Syndrome Driven by Small Fiber Neuropathy and Central Sensitization","authors":"Takahiko Nagamine","doi":"10.1111/jop.70108","DOIUrl":"10.1111/jop.70108","url":null,"abstract":"","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 4","pages":"389-390"},"PeriodicalIF":2.3,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145810370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic Significance of the Pretreatment Neutrophil-to-Lymphocyte Ratio and Tumor-Associated Neutrophils in Head and Neck Squamous Cell Carcinoma: Systematic Review and Meta-Analysis","authors":"Ahana Kapuge Dona Varuni Yashodha Ratnayake,&nbsp;Aini Hyytiäinen,&nbsp;Georgia Vasiliki Gkountana,&nbsp;Meri Torri,&nbsp;Ahmed Al-Samadi","doi":"10.1111/jop.70110","DOIUrl":"10.1111/jop.70110","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The prognostic significance of the neutrophil-to-lymphocyte ratio (NLR) and tumor-associated neutrophils (TANs) in head and neck squamous cell carcinoma (HNSCC) remains a subject of considerable research interest. This systematic review and meta-analysis aimed to evaluate the relationship between pretreatment NLR and TANs and survival outcomes in patients with HNSCC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The systematic literature search was conducted using PubMed, Scopus, Web of Science, and the Cochrane Library. Studies that documented pretreatment NLR in peripheral blood or TANs and their association with disease-specific survival (DSS), progression-free survival (PFS), overall survival (OS), disease-free survival (DFS), and recurrence-free survival (RFS) were included. Meta-analyses were performed using the R “meta” package. Subgroup analyses were performed based on tumor site and NLR thresholds. Heterogeneity was assessed using Cochran's Q and Higgins' I² statistics, while publication bias was evaluated using Egger's test and funnel plots.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 100 studies comprising 26 324 patients were included. The hazard ratios (HR) for OS, PFS, DFS, DSS, and RFS were 1.88, 1.95, 1.85, 2.16, and 1.11, respectively, in univariate analysis. Multivariate analysis supported these findings and showed similar trends. Subgroup analyses indicated that high NLR consistently predicted poor OS across all studied tumor sites and NLR thresholds. Elevated densities of TANs, particularly CD15⁺ and CXCR4⁺ subsets, were linked to poorer cancer-specific survival and OS, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This meta-analysis indicates that elevated pretreatment NLR, particularly above four, may serve as a significant prognostic marker of poor survival outcomes in patients with HNSCC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 4","pages":"458-472"},"PeriodicalIF":2.3,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13065941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145856851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Analysis of Salivary Metabolomics and Microbiota in Type 1 Diabetics With Xerostomia: A Pilot Study","authors":"Biyun Gao,&nbsp;Jinghong Luo,&nbsp;Xiaolin Nong,&nbsp;Zhaofeng Yao,&nbsp;Hairong Wang","doi":"10.1111/jop.70097","DOIUrl":"10.1111/jop.70097","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Given that the oral ecological environment undergoes alterations during the pathological progression of type 1 diabetes mellitus (T1DM) with xerostomia, this study employed microbiomics in conjunction with metabolomics to identify the distinct oral microbiota and salivary metabolites in patients with type 1 diabetic xerostomia and healthy individuals, aiming to elucidate the microorganisms, metabolites, and metabolic pathways potentially implicated in T1DM-associated xerostomia.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Saliva samples were obtained from individuals diagnosed with T1DM and xerostomia (patient group, <i>n</i> = 9) as well as from healthy volunteers (control group, <i>n</i> = 9), and were subsequently subjected to microbiota profiling and untargeted metabolomic analysis. Bioinformatics methodologies were applied to assess the microbiota and metabolomics datasets both independently and integratively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 88 differential metabolites were identified between the patient and control groups. These metabolites were predominantly enriched in the caffeine and Vitamin B6 metabolic pathways. Additionally, an average of 280 operational taxonomic units (OTUs) were detected, with species diversity found to be lower in the patient group compared to the controls. At the genus level, 20 significantly altered microbial taxa were identified. Differential genera, including <i>Leptotrichia</i> and <i>Corynebacterium</i>, exhibited higher abundance in the patient group, whereas genera such as <i>Haemophilus</i> and <i>Streptococcus</i> were predominantly present in the control group. Correlation analysis indicated that the differential microbiota did not exhibit strong associations with the identified differential metabolites. However, certain oral microorganisms were associated with specific metabolites enriched in the caffeine metabolic pathway.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>In summary, the findings demonstrated notable differences in both oral microbiota composition and salivary metabolite profiles between T1DM patients with xerostomia and healthy individuals. Furthermore, a moderate degree of association between the oral microbiome and metabolome was observed, which may serve as a potential biomarker for T1DM-related xerostomia and contribute to the development of targeted therapeutic strategies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 4","pages":"473-486"},"PeriodicalIF":2.3,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145763025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Agreement in Subepidermal Blistering Diseases: Is ELISA Test Reliable as Direct Immunofluorescence? A Systematic Review, Meta-Analysis, and Trial Sequential Analysis","authors":"Romeo Patini,&nbsp;Gioele Gioco,&nbsp;Michele Davide Mignogna,&nbsp;Stefania Leuci,&nbsp;Francesco Scilla,&nbsp;Davide Gentile,&nbsp;Cosimo Rupe,&nbsp;Enrico Russo,&nbsp;Anna Schiavelli,&nbsp;Carlo Lajolo","doi":"10.1111/jop.70088","DOIUrl":"10.1111/jop.70088","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Direct immunofluorescence (DIF) is the gold standard for diagnosing subepidermal blistering diseases (SBDs). However, DIF requires specialized expertise; therefore, alternative immunological methods such as enzyme-linked immunosorbent assays (ELISA) are worth exploring. The aim of this review was to evaluate the diagnostic agreement between DIF and ELISA for the diagnosis of SBD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A systematic literature review of international journals and electronic databases (MEDLINE via OVID, Scopus, and Web of Science) was conducted between their inception and December 2023. The risk of bias and overall quality of evidence were assessed using the Newcastle-Ottawa Scale and the GRADE system.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of the 1691 articles identified, 38 were included in the qualitative synthesis and 37 in the quantitative analysis. Three meta-analyses were developed and revealed the superiority of DIF over ELISA, with the following risk differences: BP180, 0.26 (95% CI: 0.19–0.33, <i>p</i> &lt; 0.00001, \u0000 <i>I</i>\u0000 ²\u0000 : 96%); BP230, 0.59 (95% CI: 0.47–0.71, <i>p</i> &lt; 0.00001, \u0000 <i>I</i>\u0000 ²\u0000 : 98%); and Lam332, 0.82 (95% CI: 0.70–0.94, <i>p</i> &lt; 0.00001, \u0000 <i>I</i>\u0000 ²\u0000 : 89%).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>\u0000 DIF remains the gold standard for the diagnosis of autoimmune SBDs. ELISA can serve as a complementary diagnostic tool, especially as a follow-up instrument for patients with SBD, owing to its low invasiveness.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 4","pages":"391-418"},"PeriodicalIF":2.3,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13065897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145742674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of BMI1 and ERK3 in Oral Squamous Cell Carcinoma and Their Relationship With Tissue Differentiation and Lymph Node Metastasis","authors":"Yan Zhao,&nbsp;Weiting Hao,&nbsp;Shuang Wang,&nbsp;Yingjun Li,&nbsp;Ruifang Liu,&nbsp;Sanhui Xu,&nbsp;Bo Zhang","doi":"10.1111/jop.70104","DOIUrl":"10.1111/jop.70104","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To analyze the expression of B-cell-specific Moloney murine leukemia virus insertion site 1 (BMI1) and mitogen-activated protein kinase 3 (ERK3) in the serum of oral squamous cell carcinoma (OSCC) patients and their relationship with tissue differentiation and lymph node metastasis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 200 patients with OSCC from July 2019 to October 2021 were selected as the OSCC group. And 100 healthy physical examination subjects during the same period were selected as the control group according to the 2:1 ratio principle. Serum BMI1 and ERK3 mRNA expression levels were compared between the two groups and across different clinicopathological characteristics in OSCC patients. Their correlations with pathological features were also examined. The expression levels of BMI1 and ERK3 mRNA in the serum of patients with different prognoses in the OSCC group were compared. The prognosis and survival of patients were compared based on different serum levels of BMI1 and ERK3.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The expression levels of serum BMI1 mRNA and ERK3 mRNA in the OSCC group were higher than those in the control group (<i>p</i> &lt; 0.05). There were significant differences in the expression levels of serum BMI1 mRNA and ERK3 mRNA in patients with different degrees of differentiation, lymph node metastasis, and TNM staging (<i>p</i> &lt; 0.05). The expression levels of serum BMI1 mRNA and ERK3 mRNA were negatively correlated with histological grading and positively correlated with lymph node metastasis and TNM staging (<i>p</i> &lt; 0.05). The expression levels of serum BMI1 mRNA and ERK3 mRNA in surviving patients were lower than those in deceased patients (<i>p</i> &lt; 0.05). Before and after adjusting for lymph node metastasis, TNM staging, and other factors, serum levels of BMI1 mRNA and ERK3 mRNA were still the influencing factors of OSCC prognosis (<i>p</i> &lt; 0.05). The survival rate was significantly lower in the high-expression groups than in the low-expression groups (36.2% vs. 81.3% for BMI1, 39.2% vs. 79.7% for ERK3, <i>p</i> &lt; 0.05 for both).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The expression levels of serum BMI1 mRNA and ERK3 mRNA in OSCC patients are increased, which is closely related to the pathological characteristics and prognosis of patients. BMI1 and ERK3 are helpful in evaluating the prognosis of OSCC patients.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 4","pages":"487-494"},"PeriodicalIF":2.3,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145763036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological Analysis and Survival Outcomes of Radiation-Induced Oral Squamous Cell Carcinoma: A Systematic Review and Meta-Analysis","authors":"Anne Evelyn Oliveira Moura,&nbsp;Carla Isabelly Rodrigues-Fernandes,&nbsp;Ana Gabriela Costa Normando,&nbsp;Danielle Machado Farias,&nbsp;Márcio Ajudarte Lopes,&nbsp;Pablo Agustin Vargas,&nbsp;Alan Roger Santos-Silva,&nbsp;Fábio de Abreu Alves,&nbsp;Luiz Paulo Kowalski,&nbsp;Danyel Elias da Cruz Perez","doi":"10.1111/jop.70106","DOIUrl":"10.1111/jop.70106","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Radiotherapy-induced malignancies are well-documented, but the clinicopathological profile and prognostic factors of radiation-induced oral squamous cell carcinoma (R-OSCC) remain unclear.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The present review was conducted in accordance with the PRISMA guidelines and has been registered in the PROSPERO (registration number CRD42024560015). A comprehensive search was performed across electronic databases and gray literature sources. The risk of bias was assessed using the JBI tool. Meta-analysis was performed with Review Manager 5.4.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Five cohort studies were included (<i>n</i> = 310), with four pooled in a meta-analysis. R-OSCC was significantly associated with tongue tumors (<i>p</i> = 0.007) and early clinical stages (<i>p</i> &lt; 0.0001). Negative perineural invasion was linked to sporadic OSCC (<i>p</i> = 0.0008). Smoking (<i>p</i> = 0.0008) and radiotherapy (RT) history (<i>p</i> &lt; 0.00001) were associated with decreased overall survival (OS), while RT history also reduced disease-specific survival (DSS) in R-OSCC patients (<i>p</i> = 0.006).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>R-OSCC predominantly affects the tongue, is diagnosed in early stages, and the history of RT is associated with reduced OS and DSS.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 4","pages":"448-457"},"PeriodicalIF":2.3,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13065901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145856889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Topical Application of Resveratrol Loaded Chitosan Nanoparticles on Chemically Induced Epithelial Dysplasia in Hamster Pouch","authors":"Hadeel Sirageldeen,&nbsp;Wafaa El-Hossary,&nbsp;Sara A. Swidan","doi":"10.1111/jop.70102","DOIUrl":"10.1111/jop.70102","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This research aimed to investigate the topical effect of Resveratrol (RV) loaded on chitosan nanoparticles (CS-NP) on chemically induced epithelial dysplasia in the hamster buccal pouch.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>35 Syrian hamsters were used in this study; they were divided into 4 groups. Group A (<i>n</i> = 5) was the negative control group. 7,12-dimethylbenzo anthracene (DMBA) was applied topically for 6 weeks to induce oral epithelial dysplasia in the remaining 30 animals, then they were divided into group B (<i>n</i> = 10) as the positive control group, group C (<i>n</i> = 10) animals received CS-NP (0.85 mg/100 L) topically, and group D (<i>n</i> = 10) animals received RV-CS-NP (0.85 mg/100 L) topically for another 6 weeks. Serial sections of the buccal pouches from all animals were examined histologically, and immunohistochemical (IHC) detection of the Bcl-2 gene expression was assessed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The results showed that treatment with RV loaded on CS-NPs had significantly reduced the incidence of epithelial dysplastic lesions induced by the carcinogen. Data analysis showed statistically significant differences (<i>p</i> &lt; 0.001) in the expression levels of Bcl-2 through IHC between the treatment and positive control groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Topical resveratrol-loaded chitosan nanoparticles are promising chemopreventive therapy.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 4","pages":"495-507"},"PeriodicalIF":2.3,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145810354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Electronic Nicotine Delivery Systems on Oral Mucosa: A Cytopathological and Molecular Study","authors":"Alba Pérez-Jardón,&nbsp;Bruna Fernandes do Carmo Carvalho,&nbsp;Cintia Micaela Chamorro-Petronacci,&nbsp;Maria Dolores Reboiras-López,&nbsp;Natalia de Carvalho Faria,&nbsp;Renata Falchete do Prado,&nbsp;Mónica Ghislaine Oliveira Alves,&nbsp;Fábio França Vieira e Silva,&nbsp;Maria-Elena Padín-Iruegas,&nbsp;Mario Pérez-Sayáns,&nbsp;Janete Dias Almeida","doi":"10.1111/jop.70095","DOIUrl":"10.1111/jop.70095","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The packaging and marketing of electronic cigarettes (e-cigs) often target younger demographics. This study aimed to evaluate gene expression in e-cig users through exfoliative cytology.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Samples were collected from 17 e-cig users and 10 nonsmokers as controls. Clinical data included age, gender, heart rate, oximetry, capillary blood glucose, carbon monoxide levels, sialometry, alcohol-related risk scores, alcohol consumption, and e-cig use parameters. Smears from the left tongue edge were obtained using a Rovers Orcellex Brush. The Papanicolaou method assessed epithelial maturation and cytological features, categorized from normal to conclusive for malignancy. Cellular composition, inflammation, microbial presence, and atypia were evaluated using a semiquantitative scoring system. Gene expression (p16, IL1-beta, CXCL8, TNF, and KRT13) was analyzed by RT-PCR. Statistical comparisons used the Mann–Whitney test, and correlations were assessed via Spearman's test (<i>p</i> ≤ 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Fruit flavors were the most preferred. Some users were former smokers (average abstention: 3.15 months). Bacterial colonies were more prevalent in the e-cig group (64.7% vs. 20%, <i>p</i> = 0.085), mucus and inflammatory changes were found exclusively in e-cig users (<i>p</i> = 0.062). No significant differences were found in the Papanicolaou classification by gender (<i>p</i> = 0.904). Gene expression analysis showed a differential expression of p16 and TNF between the groups. Significant correlations were found between carbon monoxide and p16 expression (<i>r</i> = −0.41, <i>p</i> = 0.02), vaping sessions per day and p16 expression (<i>r</i> = −0.37, <i>p</i> = 0.02), and daily alcohol dose and TNF expression (<i>r</i> = −0.42, <i>p</i> = 0.04).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>E-cigarette use may induce early molecular and cytological changes in the oral mucosa, affecting inflammation, immunity, and epithelial differentiation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 3","pages":"331-339"},"PeriodicalIF":2.3,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12963688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145634905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in the Oral Microbiome Between Patients With and Without Oral Squamous Cell Carcinoma","authors":"Satoshi Fukase,&nbsp;Atsumu Kouketsu,&nbsp;Toru Tamahara,&nbsp;Tatsuru Saito,&nbsp;Akiko Ito,&nbsp;Yutaro Higashi,&nbsp;Tomonari Kajita,&nbsp;Tsuyoshi Kurobane,&nbsp;Masaaki Miyakoshi,&nbsp;Masahiro Iikubo,&nbsp;Ritsuko Shimizu,&nbsp;Tetsu Takahashi,&nbsp;Kensuke Yamauchi,&nbsp;Tsuyoshi Sugiura","doi":"10.1111/jop.70099","DOIUrl":"10.1111/jop.70099","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Although studies have demonstrated a relationship between pathogenic microorganisms and oral cancer, no study has demonstrated a relationship between changes in bacterial flora and oral squamous cell carcinoma (OSCC). Therefore, we investigated the association between oral microbiota and oral squamous cell carcinoma using metagenomic analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Saliva samples from 64 patients with OSCC and 50 healthy controls who visited the Department of Oral Surgery, Tohoku University Hospital, were collected, and bacterial genomic DNA was extracted using polymerase chain reaction amplification. Single-end sequencing was performed using the Illumina MiSeq platform, and sequence data were analyzed using the Quantitative Insights Into Microbial Ecology 2 platform. The Steel–Dwass test was used for between-group comparisons, and Analysis of Compositions of Microbiomes with Bias Correction was used to detect significant differences in microbiome composition.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant differences were observed in alpha-diversity indices of bacterial flora (richness, Faith- phylogenetic diversity, Shannon index) in the OSCC group compared to those in the control group. Among the OSCC group, patients with larger tumor diameters and lymph node metastases (T3/T4, N1 or greater) formed independent clusters in the beta diversity analysis of the bacterial flora. Bacteria of the <i>Actinomycetia</i> phylum, such as <i>Actinomyces</i> and <i>Rothia</i>, were significantly reduced in patients with higher stage and pathological grade. Conversely, bacteria of the phylum <i>Spirochaetia</i> and <i>Proteobacteria</i>, particularly those of the genus <i>Treponema</i>, were significantly elevated in advanced cancer cases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our results suggest that changes in the oral microbiota may play a role in OSCC development and progression.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 3","pages":"368-380"},"PeriodicalIF":2.3,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12963696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145687701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}