Journal of Oral Pathology & Medicine最新文献

{"title":"Comment on “Diseases With Oral Malignant Potential: Need for Change to Inform Research, Policy, and Practice”","authors":"Rajiv S. Desai, Fareha Rashid, Madhura Patil, Shivani Singh","doi":"10.1111/jop.13615","DOIUrl":"10.1111/jop.13615","url":null,"abstract":"","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 4","pages":"195-196"},"PeriodicalIF":2.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting Prognostic Features in Salivary Gland Adenoid Cystic Carcinoma: Critical Appraisal and Methodological Considerations","authors":"Carlos M. Ardila, Pradeep Kumar Yadalam","doi":"10.1111/jop.13614","DOIUrl":"10.1111/jop.13614","url":null,"abstract":"","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 4","pages":"193-194"},"PeriodicalIF":2.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Y-27632 Suppresses the Growth and Migration of Oral Squamous Cell Carcinoma, but Upregulates Autophagy by Suppressing mTOR Effectors","authors":"Jie Wen,&nbsp;Yunhan Sun,&nbsp;Li Ma,&nbsp;Tingjian Zu,&nbsp;Na Wang,&nbsp;Tianqi Zhang,&nbsp;Jin Liang,&nbsp;Yulei Zhang,&nbsp;Haoyang Lu,&nbsp;Yihua Wu,&nbsp;Shizhou Zhang","doi":"10.1111/jop.13603","DOIUrl":"10.1111/jop.13603","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The Rho-associated protein kinase (ROCK) inhibitor Y-27632 is a potential immunotherapeutic agent for cancer treatment. Y-27632 blocks the growth and migration of oral squamous cell carcinoma (OSCC) CAL-27 cells. However, detailed studies on the underlying mechanisms have not yet been reported.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We investigated the effects of Y-27632 on the proliferation, migration, and invasion of OSCC cells (CAL-27, SCC-4, and SCC-9) using the Cell Counting Kit-8 assay, ethynyl-2′-deoxyuridine staining, cell scratch, and transwell assay in vitro. Next, ROCK1/2 was knocked down using siRNA to confirm that the effects of Y-27632 were mediated by the inhibition of ROCK activity. A xenograft mouse model was used to verify the effects of Y-27632 in vivo. The mechanisms underlying Y-27632-induced tumor suppression were detected using western blotting and qRT-PCR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our data demonstrated that Y-27632 potently inhibited OSCC cells (CAL-27, SCC-4, and SCC-9) by inhibiting ROCK activity. In vivo assays confirmed that Y-27632 suppressed OSCC growth by reducing cell proliferation. Biochemical assays demonstrated that Y-27632 inactivated the AKT pathway, and treatment with SC79, an AKT activator, rescued the cell growth and migration inhibition elicited by Y-27632. Further investigation revealed that Y-27632 enhanced autophagy by suppressing the AKT/mTOR pathway.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our study demonstrated that Y-27632 significantly suppressed the growth and migration of OSCC cells and upregulated autophagy via the AKT/mTOR pathway, thus providing a potential therapeutic drug for patients with OSCC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 4","pages":"207-216"},"PeriodicalIF":2.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The FUS/COL11A1/ZEB1 Axis Contributes to the Development of Oral Squamous Cell Carcinoma","authors":"Tianyuan Zhou,&nbsp;Shuang Liu,&nbsp;Lixin Shi,&nbsp;Lei Zhang","doi":"10.1111/jop.13610","DOIUrl":"10.1111/jop.13610","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Oral squamous cell carcinoma (OSCC) is a common type of head and neck cancer with high metastasis rate and poor prognosis. This study aimed to explore the role of collagen type XI alpha 1 (<i>COL11A1</i>) and its detailed mechanisms in OSCC progression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis was used to measure the level of <i>COL11A1</i>, fused in sarcoma/translocated in liposarcoma (<i>FUS</i>) and zinc finger E-box binding homeobox 1 (<i>ZEB1</i>). The protein expression of COL11A1, E-cadherin, N-cadherin, FUS, and ZEB1 were detected using Western blot. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and 5-Ethynyl-2′-deoxyuridine (EdU) staining assays were conducted to measure cell proliferation. Cell migration and invasion were evaluated via wound-healing assay and transwell migration and invasion assays, respectively. Moreover, epithelial mesenchymal transition (EMT) was determined via detecting the expression of EMT-related proteins, including E-cadherin and N-cadherin. RNA immunoprecipitation assay was conducted to evaluate the relationship between <i>ZEB1</i> and <i>COL11A1</i>. In vivo animal experiments were carried out to explore the role of <i>COL11A1</i> in OSCC mice, and immunohistochemistry assay was performed to detect the level of <i>ZEB1</i> and EMT-related proteins in tumor tissues from mice.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>\u0000 <i>COL11A1</i> was augmented in OSCC tissues and cells. <i>COL11A1</i> knockdown significantly inhibited cell proliferation, migration, invasion, and EMT in OSCC cells. <i>FUS</i> was increased in OSCC tissues. <i>FUS</i> stabilized the mRNA level of <i>COL11A1</i> and positively regulated the protein expression of COL11A1. <i>ZEB1</i> was highly expressed in OSCC tissues, and <i>COL11A1</i>directly targeted <i>ZEB1</i>. The inhibition effects of <i>COL11A1</i> knockdown on cell proliferation, invasion, migration, and EMT were reversed by <i>ZEB1</i> overexpression in OSCC cells. Additionally, <i>COL11A1</i> depletion markedly repressed tumor growth through decreasing <i>ZEB1</i> expression in vivo.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>\u0000 <i>FUS</i> stabilized <i>COL11A1</i> to promote the malignant progression of OSCC via regulating <i>ZEB1</i> expression.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 3","pages":"182-191"},"PeriodicalIF":2.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic Value of Ki-67 in the Invasive Zone of Oral Squamous Cell Carcinoma","authors":"Ke Li,&nbsp;Shuai Wang,&nbsp;Zihui Li,&nbsp;Qiuya Yu,&nbsp;Lingyun Liu,&nbsp;Fuyan Li,&nbsp;Lei Zhang,&nbsp;Guowen Sun,&nbsp;Yanhong Ni","doi":"10.1111/jop.13608","DOIUrl":"10.1111/jop.13608","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The molecular profile of cells within the tumor invasive zone, where tumor cells interact with surrounding non-tumor cells, plays a crucial role in defining tumor malignant characteristics, such as the pattern of invasion (POI). Therefore, evaluating the diagnostic value of the proliferation index molecule, Ki-67, in both tumor cells and adjacent non-tumor cells at the invasive zone with different POIs, holds significant clinical importance for oral squamous cell carcinoma (OSCC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This retrospective study included 133 primary OSCC samples, and the spatial pattern of Ki-67 in the tumor invasive zone was analyzed by immunohistochemistry (IHC). The prognostic value of tumor cells and stroma proliferative capacity in different POIs were assessed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Ki-67 was widely expressed in tumor cells and stroma cells within the invasive zone, and cells in high-invasiveness POIs exhibit higher proliferation. Elevated Ki-67 expression in tumor cells was associated with poor overall survival (OS) and disease-free survival (DFS) in patients, which is independent of POIs. In our study, we identified the expression level of Ki-67 in tumor cells across high-invasiveness POIs as an independent risk factor for OS and DFS in OSCC patiens. Additionally, Ki-67 expression in surrounding non-tumor cells did not significantly correlate with patient survival.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The remarkable proliferation characteristic of tumor cells in high-invasiveness POIs of OSCC tumors plays a crucial role in the prognosis of patients.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 3","pages":"173-181"},"PeriodicalIF":2.7,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiologic Specific Growth Rate of Ameloblastomas: A Clinicopathological Correlation","authors":"Chané Smit,&nbsp;Liam Robinson,&nbsp;Marlene B. van Heerden,&nbsp;Pieter W. Meyer,&nbsp;Felipe P. Fonseca,&nbsp;Willie F. P. van Heerden,&nbsp;André Uys","doi":"10.1111/jop.13611","DOIUrl":"10.1111/jop.13611","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The study aimed to assess the radiologic-specific growth rate of ameloblastomas, evaluating potential associations with demographics, radiologic features, histopathologic variants and proliferation indices. The results of this study will hopefully establish if any clinical or histopathologic features can elude fast-growing ameloblastomas.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Patients presenting with multiple radiographs before surgical intervention due to various healthcare constraints or patient factors were included in the study. The measurements from each radiograph included the lesion's length, height, width and amount of expansion in these dimensions. Furthermore, the circumference of the lesion was measured in sagittal, coronal and axial planes. The radiologic-specific growth rate was assessed by calculating the difference in measurements from the initial to follow-up radiographs divided by the duration between the visits to calculate the growth rate per year.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The specific growth rate was analysed between age groups, histopathologic variants and Ki-67 values, with no statistically significant correlations found in all dimensions measured. A statistically significant faster growth (<i>p</i> = 0.04) was seen in females when measuring the mesial-distal length. When comparing radiologic features, ameloblastomas with loss of border demarcation, severe cortical destruction and tooth displacement demonstrated statistically significant faster growth.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study found significant correlations with the growth rate of ameloblastomas, specifically in coronal dimensions, supporting the notion of buccal-lingual growth/expansion for which ameloblastomas are known.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 3","pages":"161-172"},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.13611","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adverse Reactions to Cosmetic Fillers in the Oral and Maxillofacial Region: Clinico-Pathological, Histochemical, and Immunohistochemical Characterization","authors":"Ana Cristina Tetzner,&nbsp;Laura Regina Mendes Viana,&nbsp;Lucas Guimarães Abreu,&nbsp;Elismauro Francisco Mendonça,&nbsp;Diego Antônio Costa Arantes,&nbsp;Ana Carolina Uchoa Vasconcelos,&nbsp;Ana Paula Neutzling Gomes,&nbsp;Cassiano Francisco Weege Nonaka,&nbsp;Pollianna Muniz Alves,&nbsp;Roberto Onner Cruz Tapia,&nbsp;Ricardo Alves Mesquita,&nbsp;Sílvia Ferreira de Sousa,&nbsp;Tarcília Aparecida Silva,&nbsp;Patrícia Carlos Caldeira","doi":"10.1111/jop.13604","DOIUrl":"10.1111/jop.13604","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Cosmetic injections are increasing, as their complications, which can be misdiagnosed as neoplastic lesions. This study aimed to detail clinical, pathological, histochemical, and immunohistochemical features of adverse reactions to cosmetic fillers in the oral and maxillofacial region.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Samples were retrieved from five pathology laboratories. Hematoxylin–eosin (H&amp;E), Alcian Blue, Sirius Red, and Toluidine blue stains were performed, as well as immunohistochemistry for CD68, CD3, and CD20. H&amp;E was evaluated under polarization. Descriptive statistics were performed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Twenty-three cases were included. Polymethyl-methacrylate was the most common material. Most reactions affected women, lips and were asymptomatic, with a variable time of evolution, presenting as nodules. Materials had different shape and size on H&amp;E. Giant cells were commonly found, except in silicone and hyaluronic acid. Foreign-body granuloma was frequent in polymethyl-methacrylate. Calcium hydroxyapatite and poly-L-lactic acid were refractile under polarized light. Hyaluronic acid and polyacrylamide hydrogel were metachromatic by Toluidine blue. Alcian blue was positive in all cases of hyaluronic acid. Mast cells were detected in all materials, except hyaluronic acid and polyacrylamide hydrogel. Eosinophils were rarer than mast cells. Numerous CD68-positive cells were seen in all cases. All cases had CD3-positive cells, with variable amounts. CD20 was scant or negative in most cases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>An evident macrophage reaction is observed in all aesthetic fillers, frequently associated with giant cell formation. Despite similarities, there are specific features of each material and the host response that assist the correct histopathological diagnosis. Immunohistochemistry for CD68 and Toluidine blue stain are useful in doubtful cases.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 3","pages":"141-150"},"PeriodicalIF":2.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of TNS3-203 and LRRFIP1-211 Transcripts as Oral Cancer Biomarkers","authors":"Katarina Zeljic,&nbsp;Dunja Pavlovic,&nbsp;Goran Stojkovic,&nbsp;Sandra Dragicevic,&nbsp;Jelena Ljubicic,&nbsp;Nikola Todorovic,&nbsp;Aleksandra Nikolic","doi":"10.1111/jop.13606","DOIUrl":"10.1111/jop.13606","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>A recent pan-cancer transcriptome analysis indicated differential activity of alternative promoters of genes <i>TNS3</i> and <i>LRRFIP1</i> in head and neck squamous cell carcinoma compared to non-cancerous tissue. The promoters upregulated in head and neck squamous cell carcinoma regulate expression of transcripts TNS3-203 and LRRFIP1-211.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Our aim was to investigate the biomarker potential of TNS3-203 and LRRFIP1-211 transcripts in oral cancer, the most common type of head and neck cancer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>An in silico approach was used to characterize the promoters and transcripts of interest. Relative expression of TNS3-203 and LRRFIP1-211 transcripts was evaluated by qRT-PCR in a group of 46 oral cancer patients in samples of cancer and adjacent non-cancerous tissue.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>TNS3-203 was significantly overexpressed in oral cancer compared with matched non-cancerous tissue, so this transcript can potentially be used as a diagnostic biomarker. There were no differences in LRRFIP1-211 level between analyzed tissues. None of the investigated transcripts has prognostic potential in oral cancer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The results obtained indicate the diagnostic potential of TNS3-203, but not LRRFIP1-211 transcript and its role in oral carcinogenesis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 3","pages":"151-160"},"PeriodicalIF":2.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boswellia Extract Promotes Healing and Resolving Inflammation in Oral Ulcers of Rat","authors":"Wei Zhao,&nbsp;Zhuoqun Jia,&nbsp;Jiao Han,&nbsp;Xiaojun Sun","doi":"10.1111/jop.13609","DOIUrl":"10.1111/jop.13609","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Recurrent aphthous ulcers significantly impact patients' quality of life due to their painful and recurrent nature, necessitating more effective treatments. This study explores the therapeutic potential of Boswellia to treat recurrent aphthous ulcers by its anti-inflammatory and healing promotion effect in a rat oral ulcer model.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Network pharmacology techniques were employed to elucidate Boswellia's active components and potential targets. Intersecting targets of Boswellia and oral ulcer-related genes were screened for protein–protein interaction network analysis and functional enrichment. An oral ulcer model in rats was used and rats were treated with Boswellia extract. The healing process was monitored by measuring the ulcer area and body weight changes. Histological analysis was performed, and the role of Boswellia in macrophage polarization was investigated through gene expression analysis and protein array tests. The underlying mechanism involving PPARγ activation was also explored.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Network pharmacology analysis revealed Boswellia's interaction with key genes and pathways associated with inflammation and lipid metabolism, such as MAPK3, PPARG, and PTGS2. Boswellia extract significantly accelerated oral ulcer healing and recovered weight loss in rats. Histological examinations revealed reduced tissue swelling and inflammatory cell infiltration in treated groups. Furthermore, Boswellia extract decreased infiltration of M1 macrophage presence while increasing M2 macrophage, indicating an inflammation-resolving effect. In vitro studies showed that Boswellia extract enhanced M2-related gene expression and decreased pro-inflammatory cytokines, which is PPARγ dependent.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Boswellia extract promotes oral ulcer healing and resolves inflammation, primarily through the modulation of macrophage polarization via PPARγ activation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 3","pages":"131-140"},"PeriodicalIF":2.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PETRICCS Guideline Protocol: A Call to Improve Reporting Standards in Cell Culture Research","authors":"Mylene Martins Monteiro,&nbsp;Juliana Amorim dos Santos,&nbsp;Victor Paiva Barbosa,&nbsp;Graziela De Luca Canto,&nbsp;Cristiane H. Squarize,&nbsp;Ricardo D. Coletta,&nbsp;Eliete Neves Silva Guerra","doi":"10.1111/jop.13605","DOIUrl":"10.1111/jop.13605","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Cell culture studies play an important role in addressing fundamental scientific questions. However, inadequate reporting of these studies results in a lack of transparency and reproducibility. Recognizing the need for improvement, several ongoing efforts, such as CRIS guidelines and the ICLAC checklist, are focused on enhancing best practices for in vitro studies. Nonetheless, a comprehensive guideline specifically addressing the reporting of cell culture methods remains lacking. In this manner, a consensus-based approach is being undertaken to develop the Preferred and Transparent Reporting Items for Cell Culture Studies (PETRICCS) guideline. This project aims to present the protocol and the details for its development.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The process comprises five phases: (i) Initial Steps: a Steering Committee identifies the need for a guideline and drafts the PETRICCS protocol; (ii) Pre-meeting: an International Group of Cell Culture Experts (IGCE) reviews the draft guideline through a Delphi consensus exercise; (iii) Consensus Meeting: the steering committee presents the guideline's development, addresses concerns, and reaches consensus on the final items; (iv) Post-meeting: explanatory documents are prepared to assist authors in reporting their findings; and (v) Post-publication: PETRICCS, along with supporting documents, is published and made freely accessible.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results and Conclusions</h3>\u0000 \u0000 <p>PETRICCS will assist researchers in reporting and reviewing cell culture findings, enhancing transparency and reproducibility while filling a gap in this crucial scientific field. The guideline will incorporate the experiences of experts, creating a more equitable environment for authors, peer-reviewers, and editors during the publication process.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 2","pages":"112-119"},"PeriodicalIF":2.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}