Journal of Oral Pathology & Medicine最新文献

{"title":"Oral Changes in Patients Undergoing Hematopoietic Stem Cell Transplantation: A Cohort Study","authors":"Tatiana Bernardo Farias Pereira,&nbsp;Gleidston Silva Potter,&nbsp;Beatriz Maria Falcão Lima,&nbsp;Ana Rafaela Luz de Aquino Martins,&nbsp;Maria Luiza Diniz de Sousa Lopes,&nbsp;Kenio Costa de Lima,&nbsp;Éricka Janine Dantas da Silveira","doi":"10.1111/jop.13629","DOIUrl":"10.1111/jop.13629","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Hematopoietic stem cell transplantation (HSCT) is an important and potentially curative treatment for some hematological disorders. Chemotherapeutic preconditioning can result in complications due to the direct or indirect toxicities of the administered drugs. Among the adverse effects, various complications may arise within the oral environment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>We investigated the occurrence of oral alterations in hematologic patients during their hospitalization for HSCT.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study involved 30 patients undergoing HSCT at a reference hospital in Brazil. Data on oral physical examination, hematological disorder diagnosis, transplant type, comorbidities, chemotherapy protocols, and oral risk factors were collected. Survival analysis was conducted to estimate the onset time of oral alterations and investigate potential associations with risk factors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Oral alterations were observed in 93.3% of patients, with the most common being edema of the buccal mucosa (83.3%) and oral mucositis (80%). FluBuMel was the most frequently used conditioning protocol (46.7%). The mean follow-up time was 23 days, and the probability of the patient remaining free of oral alterations decreased as time progressed. The Mel200 protocol (HR 2.89; IC 0.04–1.02; <i>p</i> = 0.020) and autologous transplant (HR 3.41; CI 1.28–9.07; <i>p</i> = 0.004) were associated with an earlier occurrence of oral alterations, while allogenic related transplant was a protective factor (HR 0.48; CI 0.22–1.07; <i>p</i> = 0.040).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The time of onset of oral alterations is affected by transplant type and conditioning protocol, and suggests that the severity of the alterations is positively influenced by the presence of dentists in the HSCT team.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 5","pages":"351-359"},"PeriodicalIF":2.7,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Longitudinal Study of E-Cadherin and Beta-Catenin in Progression of Oral Epithelial Dysplasia","authors":"Ilena S. Yim,&nbsp;Lewei Zhang,&nbsp;Leigha D. Rock,&nbsp;Miriam P. Rosin,&nbsp;Iris Lin,&nbsp;Denise M. Laronde","doi":"10.1111/jop.13622","DOIUrl":"10.1111/jop.13622","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>This study explored the expression patterns of epithelial-mesenchymal transition markers E-cadherin and beta-catenin in mild and moderate oral epithelial dysplasia (OED) to determine whether their expression predicts malignant progression in oral tissue.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Formalin-fixed paraffin-embedded tissue specimens with mild or moderate dysplasia were retrieved from 87 patients. Immunohistochemistry was performed to compare E-cadherin and beta-catenin expression in tissue sections that progressed to severe dysplasia, carcinoma in situ, or squamous cell carcinoma (<i>n</i> = 29) with those that did not progress (<i>n</i> = 58). Expression patterns were observed in the basal, parabasal, lower spinous, and upper spinous epithelial layers. Expression was assessed in the cell membrane for E-cadherin and beta-catenin (low expression = absent/weak staining, high = moderate/strong) and in the cytoplasm and nucleus for beta-catenin (low = absence, high = presence). Logistic regression was used to predict progression based on the expression pattern.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>There were no significant differences in the progression and expression patterns of E-cadherin and beta-catenin (<i>p</i> &gt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study found that the expression of E-cadherin and beta-catenin was not a predictor of early malignant progression, highlighting the importance of longitudinal studies in studying progression.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 5","pages":"334-342"},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.13622","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and Clinical Characteristics of Oral Squamous Cell Carcinoma Analyzed by Digitally Assembled Whole-Mount Sections","authors":"Han Gyeol Kim,&nbsp;Nayeon Choi,&nbsp;Manki Chung,&nbsp;Han-Sin Jeong,&nbsp;Junhun Cho","doi":"10.1111/jop.13630","DOIUrl":"10.1111/jop.13630","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The diagnosis of oral squamous cell carcinoma (OSqCC) is sometimes delayed. In this study, we attempted to comprehensively analyze the surface and cross section of OSqCC using whole-mount sections.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Representative cross sections of 41 pT2 and pT3 OSqCC were digitally assembled, and whole-mount sections were reconstructed. In the cross section of the tumor, the degree of differentiation was classified as well/moderately differentiated (WDMD) and poorly differentiated (PD); and the surface of the specimen was divided into four types: benign-looking epithelium, dysplasia, carcinoma, and ulceration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In the cross-sectional analysis, 17 cases (41.5%) consisted of only WDMD, and the PD component was predominant in seven cases (17.1%). In the remaining 17 patients, the PD component was partial (&lt; 50%). When grouping noncancerous lesions (benign-looking epithelium and dysplasia, BnDy) and cancerous lesions (carcinoma and ulceration, CaUl) in the surface analysis, the proportion of CaUl was significantly lower in the tongue than in other oral sites (<i>p</i> = 0.009) In the survival analysis, although not statistically significant, the overall survival of partial PD patients was more similar to that of the predominant PD group compared to that of the WDMD-only group.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 5","pages":"343-350"},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomarkers in Peri-Implant Crevicular Fluid of Healthy Implants and Those With Peri-Implant Diseases: A Systematic Review and Meta-Analysis","authors":"Gerardo La Monaca,&nbsp;Nicola Pranno,&nbsp;Romeo Patini,&nbsp;Antonella Polimeni,&nbsp;Massimo Cordaro,&nbsp;Maria Paola Cristalli","doi":"10.1111/jop.13612","DOIUrl":"10.1111/jop.13612","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Several biomarkers in peri-implant crevicular fluid have been studied to diagnose peri-implant diseases with inconclusive results. This systematic review and meta-analysis aimed to comprehensively compare data on the levels of biological components in peri-implant crevicular fluid collected from healthy and diseased implants to identify reliable biomarkers for diagnosing and monitoring peri-implant disease.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>The search strategy included studies comparing biomarker levels in peri-implant crevicular fluid between healthy and diseased implants through electronic databases (MEDLINE/PubMed, Embase, Cochrane Library), grey literature, and hand-searching relevant journals and reference lists of pertinent papers. A two-stage screening was performed in duplicate and independently. In the first stage, titles and abstracts that fulfilled eligibility criteria were screened. In the second stage, a full-text analysis was conducted to verify eligibility. All articles meeting the inclusion criteria underwent data extraction and quality assessment. Meta-analyses were conducted on studies with similar comparisons and outcome measures.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>After screening the titles and abstracts, out of 100 potentially relevant papers identified for full-text evaluation, 49 were excluded, 51 were included in the qualitative analysis, and 18 were included in the quantitative synthesis. Among 96 biomarkers assessed, the most studied were pro-inflammatory cytokines (IL-1ß, IL-6, TNF-α, and IL-17), osteoclastogenic-related factors (RANK, RANKL, and OPG), anti-inflammatory cytokines (IL-10), chemokines (IL-8, MIP-1α/CCL3, and MIP-3α/CCL-20), and enzymes (MMP-8, Cat-K, AST, and ALT).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Meta-analyses comparing data from healthy patients and those with peri-implantitis or mucositis and between patients with mucositis and those with peri-implantitis showed a moderate predictive value of IL-1ß, VEGF, cortisol, and sRANKL/OPG for peri-implantitis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 5","pages":"267-282"},"PeriodicalIF":2.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.13612","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Oral Topical Capsaicin Gel on Taste Perception in Healthy Subjects: A Pilot Study","authors":"Katia Rupel,&nbsp;Alex Buoite Stella,&nbsp;Martina Tamos,&nbsp;Daniela Adamo,&nbsp;Federica Canfora,&nbsp;Matteo Biasotto,&nbsp;Roberto Di Lenarda,&nbsp;Giulia Ottaviani","doi":"10.1111/jop.13620","DOIUrl":"10.1111/jop.13620","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Topical capsaicin is widely used for managing peripheral neuropathies; however, its impact on gustatory perception following prolonged oral use remains unclear. This pilot study aimed to evaluate changes in gustatory sensitivity and food preferences induced by capsaicin topical gel therapy in healthy individuals.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Ten healthy female subjects applied capsaicin gel (0.025%) to the gingival mucosa twice daily for 14 days. Evaluations were conducted at baseline (T0), after 2 weeks (T1), after 4 weeks (T2), and after 4 weeks following discontinuation (T3). A matched control group underwent identical assessment without capsaicin application. Gustatory changes were measured using a modified taste strip method and a food preferences questionnaire.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>While subjective alterations in food perception, liking, and preferences were reported in the capsaicin group, no significant objective changes in gustatory perception (intensity and recognition of salty, sweet, sour, and bitter flavors) were observed. Subjective changes were reversible upon cessation of capsaicin use.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Topical capsaicin gel influences subjective food perception and preferences without objectively altering gustatory function. These findings highlight the importance of considering such effects when prescribing capsaicin for oral somatosensory disorders, such as burning mouth syndrome or dysgeusia.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 5","pages":"392-396"},"PeriodicalIF":2.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.13620","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Exosomal miR-320d/FAM49B Axis by Guanylate Binding Protein 5 Promotes Cell Growth and Tumor Progression in Oral Squamous Cell Carcinoma","authors":"Kai-Fang Hu,&nbsp;Chih-Wen Shu,&nbsp;Chun-Feng Chen,&nbsp;Cheng-Hsin Lee,&nbsp;Hsiang-Chien Kung,&nbsp;Yu-Hsiang Chou,&nbsp;Chun-Lin Chen,&nbsp;Pei-Feng Liu","doi":"10.1111/jop.13624","DOIUrl":"10.1111/jop.13624","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Guanylate binding protein 5 (GBP5) and exosomal miRNAs are involved in tumor progression. While several studies reveal the connection between GBP5 and exosomes for immune response and infection, this relationship in cancer, particularly in oral squamous cell carcinoma (OSCC), remains unexplored.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The exosomal miRNA extracted from the cells was analyzed using next-generation sequencing. Bioinformatic tools were used to predict exosomal miRNA target genes. OSCC cell growth was verified by colony formation, cell viability, and cell cycle analysis. The Cancer Genome Atlas database was used to inspect the prognosis of OSCC patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our results showed that OSCC cells treated with exosomes from GBP5-silenced OSCC cells reduced colony formation. Also, 56 differentially expressed exosomal miRNAs were found in GBP5-silenced OSCC cells compared to scrambled OSCC cells. Among them, exosomal miR-320d exhibited the highest negative correlation with GBP5 in OSCC patients. High GBP5/low miR-320d co-expression was linked to reduced disease-free survival (DFS) in patients with OSCC. Interestingly, the inhibitory effect of GBP5-silenced exosomes on OSCC cell growth was reversed by miR-320d inhibitors. Moreover, five miR-320d target genes were predicted, and only Family with Sequence Similarity 49, Member B (FAM49B) showed a negative correlation with miR-320d. A decreased level of FAM49B was found in OSCC cells treated with exosomes derived from GBP5-silenced OSCC cells, while the decreased level of FAM49B was reversed by miR-320d inhibitors. Silencing FAM49B and GBP5-silenced exosomes enhanced the cytotoxicity of paclitaxel. FAM49B was abundantly expressed in tumor tissues, and high FAM49B/low miR-320d and high GBP5/high FAM49B co-expression were linked to reduced DFS of OSCC patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our study suggests that GBP5 downregulated exosomal miR-320d may trigger FAM49B expression and facilitate OSCC tumor growth and progression.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 5","pages":"298-311"},"PeriodicalIF":2.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV-2 Entry Factors ACE2, TMPRSS2 and FURIN in Salivary Glands From Primary Sjögren's Disease","authors":"Fernanda Aragão Felix,&nbsp;Flávia Martins Vasconcelos Filiú,&nbsp;Tarcília Aparecida da Silva,&nbsp;Débora Cerqueira Calderaro,&nbsp;Leandro Augusto Tanure,&nbsp;Maurício Augusto Aquino de Castro,&nbsp;Ricardo Santiago Gomez,&nbsp;Marina Gonçalves Diniz,&nbsp;Sílvia Ferreira de Sousa","doi":"10.1111/jop.13621","DOIUrl":"10.1111/jop.13621","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Salivary glands are the reservoir for severe acute respiratory syndrome Coronavirus 2 virus (SARS-CoV-2). It is known that SARS-CoV-2 uses angiotensin-converting enzyme 2 (ACE2), transmembrane serine protease 2 (TMPRSS2) and FURIN to infect cells. Salivary glands express these entry factors, and it is unclear if these molecules are expressed in salivary glands from primary Sjögren's disease (pSjD). This study assessed <i>ACE2</i>, <i>TMPRSS2</i>, and <i>FURIN</i> gene expression by RT-qPCR in pSjD compared to normal salivary gland and pleomorphic adenoma.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>An integrated study of <i>ACE2</i>, <i>TMPRSS2</i>, and <i>FURIN</i> gene expression across pSjD, pleomorphic adenoma, and normal salivary gland by RT-pPCR was performed. The association between gene expression and clinicopathological data was also examined.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>pSjD showed ACE2 upregulation compared with normal salivary gland and pleomorphic adenoma (<i>p</i> &lt; 0.05). <i>TMPRSS2</i> and <i>FURIN</i> mRNA levels are also higher in pSjD compared to pleomorphic adenoma (<i>p</i> &lt; 0.05). <i>ACE2</i> expression levels were associated with anti-SSA/SSB positivity (<i>p</i> &lt; 0.05). A strong correlation was observed between <i>TMPRSS2</i> and <i>FURIN</i> in pSjD (<i>r</i> = 0.768; <i>p</i> = 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our analysis revealed a high expression of <i>ACE2</i> in pSjD. <i>ACE2</i>, <i>TMPRSS2</i>, and <i>FURIN</i> mRNA levels are increased in pSjD in comparison to pleomorphic adenomas. Neither <i>ACE2</i>, <i>TMPRSS2</i>, nor <i>furin</i> was consistently associated with SARS-CoV-2 positivity in pSjD. These findings may guide future investigations on the effects of SARS-CoV-2 in a subset of salivary gland diseases.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 5","pages":"290-297"},"PeriodicalIF":2.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AMPK in Chemoradiotherapy-Induced Oral Mucositis","authors":"Junjie Jiang,&nbsp;Hao Xu,&nbsp;Mingyue Liu,&nbsp;Jiwei Guo,&nbsp;Jing Li,&nbsp;Jianwen Li,&nbsp;Hengtai Bi,&nbsp;Yousen Wang,&nbsp;Zhiliang Wang","doi":"10.1111/jop.13626","DOIUrl":"10.1111/jop.13626","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Oral mucositis (OM) is a prevalent adverse effect of radiotherapy and chemotherapy, significantly impacting cancer patients' well-being and potentially increasing mortality rates. Understanding OM's pathogenesis and identifying effective preventative and therapeutic agents are clinically crucial.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study analyzed RNA-Seq data from the GEO database, focusing on OM samples post-radiotherapy and chemotherapy. Differential gene expression analysis between OM and non-OM groups, followed by gene ontology (GO) enrichment analysis of differentially expressed genes (DEGs), was conducted. LASSO regression identified five potential biomarkers, and CIBERSORT assessed immune infiltration in OM samples. Correlations between biomarkers and immune infiltration were explored, and the connectivity map (CMAP) screened potential therapeutic drugs. The top 10 drugs were validated through molecular docking.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 47 DEGs were identified, primarily involved in mitotic sister chromatid separation according to GO enrichment analysis. CIBERSORT analysis revealed significant changes in B cell naive and dendriform cells (DCs) resting content in the OM group. PRKAA2, encoding the AMP-activated protein kinase (AMPK) catalytic subunit, showed a negative correlation with DC resting content. Molecular docking from CMAP identified aloisine and teniposide as potential agents for OM induced by radiotherapy and chemotherapy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>AMPK emerges as a crucial regulator in OM post radiotherapy and chemotherapy, implicating sister chromatid separation, where DCs may play a pivotal role. Aloisine and Teniposide appear promising for OM prevention or treatment associated with these treatments.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 5","pages":"325-333"},"PeriodicalIF":2.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triptolide Treatment for Oral Squamous Cell Carcinoma by Regulating the LncRNA-MSTRG.24214.1/MiRNA-939-5p/LCN2 Axis","authors":"Siyan Chen,&nbsp;Zhengmiao Li,&nbsp;Menglin Hu,&nbsp;Yang Yu,&nbsp;Bing Liu,&nbsp;Wuliji Saiyin,&nbsp;Jichen Li","doi":"10.1111/jop.13625","DOIUrl":"10.1111/jop.13625","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Although triptolide has demonstrated efficacy in treating oral squamous cell carcinoma (OSCC), its precise molecular mechanism remains unclear. This study investigated the mechanism underlying triptolide's action in lncRNA-mediated competing endogenous RNA (ceRNA) regulation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The impact of triptolide on OSCC in vivo was validated using a xenograft tumor model. Whole-transcriptome sequencing and bioinformatics analysis were conducted to construct the lncRNA-miRNA-mRNA regulatory network. Relative gene and protein expression levels were confirmed using qRT-PCR and Western blot. Dual-luciferase assays were performed to assess target interactions, while cell proliferation was measured using CCK8 assays, and cell migration and invasion were evaluated via wound healing and transwell assays.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Triptolide markedly reduced proliferation, migration, and invasion in Cal27 and Tca8113 cells. After 22 days of triptolide treatment, the tumor volume of mice gradually shrank. This led to significant upregulation of cleaved Caspase-3 and Bax, alongside downregulation of Bcl-2. Transcriptome sequencing and bioinformatics analysis identified 266 differentially expressed mRNAs, 528 lncRNAs, and 85 miRNAs. Enhanced expression of lncRNA MSTRG.24214.1 and mRNA LCN2, along with reduced expression of miR-939-5p, was observed in the triptolide group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The lncRNA-miRNA-mRNA ceRNA network associated with triptolide's impact on OSCC was successfully established. Triptolide suppressed OSCC development and progression both in vitro and in vivo, potentially through modulation of the MSTRG.24214.1-miR-939-5p-LCN2 axis. These findings offer a solid foundation for future personalized triptolide-based therapeutic approaches.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 5","pages":"312-324"},"PeriodicalIF":2.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Royal Jelly as a Therapeutic Intervention in Medication-Related Osteonecrosis of the Jaw (MRONJ): An Animal Model Study","authors":"Cihan Topan,&nbsp;Suheyb Bilge,&nbsp;Ahmet Emin Demirbas,&nbsp;Gürkan Ağyüz,&nbsp;Ayça Kara","doi":"10.1111/jop.13627","DOIUrl":"10.1111/jop.13627","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>To evaluate the efficacy of royal jelly in managing experimentally created MRONJ model in rats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Sixty rats were randomly allocated into control, bisphosphonate (BP), royal jelly (RJ), Treatment, and Preventive groups. A defect was created in the alveolar socket following tooth extraction in the mandible as a surgical procedure in all groups. Before surgery, RJ was administered orally to the RJ group. Zoledronic acid was administered intraperitoneally to induce osteonecrosis in BP, treatment, and preventive group rats. Treatment group rats received RJ orally post-surgery, while preventive group rats received it pre-surgery. Histological and radiographic evaluations were performed post-study completion.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Micro-CT examinations demonstrated significantly improved values in RJ-received groups (RJ, treatment, and preventive) compared to BP and control groups (<i>p</i> &lt; 0.001). Immunohistochemical analysis revealed higher mean IL-1β and TNF-α levels in the BP group. The highest IL-1β difference was between BP and preventive groups (<i>p</i> &lt; 0.001). TNF-α expression levels in all RJ-received groups were comparatively close to those of the control group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>RJ enhances soft and hard tissue healing in MRONJ rat models, suggesting its potential as a therapeutic or preventive agent in osteonecrosis management.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"54 4","pages":"232-240"},"PeriodicalIF":2.7,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}