Journal of Oral Pathology & Medicine最新文献

{"title":"Long-Term Care of OLP Patients: A Retrospective Study From a Single Hospital Outpatient Clinic.","authors":"F Scilla, G Gioco, L Ferrero, R Castagnola, A Zafarone, L Cimino, M Tranfa, C Rupe, C Lajolo","doi":"10.1111/jop.70179","DOIUrl":"https://doi.org/10.1111/jop.70179","url":null,"abstract":"<p><strong>Background: </strong>Oral lichen planus (OLP) is a chronic immune-mediated disorder with malignant potential. However, its real-world clinical course remains incompletely characterized. This study aimed to describe the natural clinical-histopathological trajectory of OLP in a single tertiary centre and to identify factors associated with dysplasia and malignant transformation over time.</p><p><strong>Methods: </strong>We conducted a single-centre retrospective cohort study including patients diagnosed with OLP and followed between 2007 and 2024 at the Oral Medicine Unit of Fondazione Policlinico Universitario A. Gemelli IRCCS. Demographic, clinical (red vs. white phenotype and lesion distribution), microbiological (Candida spp.), therapeutic, and histopathological variables were collected across follow-up. Univariate and multivariable logistic regression models were used to evaluate factors associated with dysplasia and malignant transformation.</p><p><strong>Results: </strong>Among 308 screened patients, 246 met inclusion criteria (173 women, 70.3%; mean age 63.2 ± 15.0 years) with a mean follow-up of 55.5 ± 37.0 months. At baseline, Candida spp. colonization was detected in 55 patients (22.4%) and was more frequent in red-type OLP than white-type lesions (33.3% vs. 17.0%; OR 2.44, 95% CI 1.32-4.53; p = 0.005). During follow-up, 33 patients (13.4%) developed fungal infection despite an initially negative swab. Epithelial dysplasia emerged in 42 patients (17.1%). In multivariable analysis, dysplasia was independently associated with increasing age (OR 1.06, 95% CI 1.03-1.10; p = 0.0001) and red-type OLP(OR 2.24, 95% CI 1.10-4.55; p = 0.026). Eight patients (3.25%) developed infiltrative OSCC after a mean interval of 28.3 months from OLP diagnosis (range 4-55).</p><p><strong>Conclusions: </strong>In this long-term single-centre cohort, OLP frequently evolved through dysplasia during follow-up, and malignant transformation occurred in a minority of patients. Older age and red-type phenotype characterized patients more likely to develop dysplasia, while malignant transformation was more frequent in the case of previously documented dysplastic lesions, in keeping with the notion of dysplasia as a step along the carcinogenic pathway, older age, and diffuse lesions.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tempol Protects Against Radiation Injury in the Submandibular Gland Through the β-Catenin Signaling Pathway","authors":"Jinfeng Wang,&nbsp;Mingjun Xu,&nbsp;Jinghua Zhong,&nbsp;Kui Zhong,&nbsp;Zheng Chen,&nbsp;Chunyu Liu,&nbsp;Chao Liu","doi":"10.1111/jop.70147","DOIUrl":"10.1111/jop.70147","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Radiation damage severely impacts salivary gland function and cell survival, especially in head and neck radiotherapy. Tempol (TPL), a free radical scavenger, has shown protective effects against radiation damage. This research aimed to investigate the protective effects of TPL on radiation-induced damage in Hs917.T cells and the submandibular gland (SMG) of C57BL/6 mice, along with the mechanisms involved.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Human parotid fibroblasts (Hs917.T) were pre-treated with TPL and exposed to ionizing radiation (IR). Protective effects were evaluated using MTT, clonogenic survival assays, flow cytometry, and intracellular reactive oxygen species levels. In vivo, C57BL/6 mice were pre-treated with TPL (275 mg/kg) and exposed to 15 gray (Gy). Effects were assessed by survival rates, body weight changes, histological analysis, and TUNEL staining. Changes in apoptosis-related markers and β-catenin signaling pathway were analyzed, and the role of TPL was verified using the β-catenin inhibitor XAV939.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>TPL pre-treatment increased cell survival, reduced apoptosis, alleviated cell cycle arrest, and decreased intracellular superoxide and hydrogen peroxide levels in Hs917. T cells. In C57BL/6 mice, pre-treatment with TPL improved survival, mitigated weight loss, reduced SMG damage, and decreased apoptosis. TPL inhibited IR-induced apoptosis by increasing Bcl-2 expression and decreasing Bax and caspase-9 levels. TPL exerted anti-apoptotic and protective effects by upregulating the expression of β-catenin, promoting its nuclear translocation, and inhibiting its phosphorylation. These protective effects of TPL were reversed by XAV939.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>TPL exerted protective effects against IR-induced damage in Hs917.T cells and the SMG of C57BL/6 mice through activating the β-catenin signaling pathway, inhibiting cell apoptosis, and alleviating oxidative stress.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 8","pages":"895-905"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147941487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterogeneity of miRNAs in Salivary Gland Neoplasms: An Exploratory Pilot Study","authors":"Dandara Andrade de Santana,&nbsp;Poliana Ramos Braga,&nbsp;Daniel Araki Ribeiro,&nbsp;Patricia Ramos Cury,&nbsp;Ana Carolina Velasco Pondé de Sena,&nbsp;Flávia Caló de Aquino Xavier,&nbsp;Fernando Augusto Soares,&nbsp;Iguaracyra Barreto de Araújo,&nbsp;Fabio Albuquerque Marchi,&nbsp;Valéria Souza Freitas,&nbsp;Cláudia Malheiros Coutinho-Camillo,&nbsp;Jean Nunes dos Santos","doi":"10.1111/jop.70150","DOIUrl":"10.1111/jop.70150","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>MicroRNAs (miRNAs) are small RNAs that have been associated with tumourigenesis and tumour progression and that play an important role in the pathogenesis of salivary gland neoplasms. The aim of this study was to evaluate the global miRNA expression profile in a sample of salivary gland neoplasms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Cases of pleomorphic adenoma (<i>n</i> = 4), adenoid cystic carcinoma (<i>n</i> = 4) and mucoepidermoid carcinoma (<i>n</i> = 4) were studied by real-time RT-PCR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The main up-regulated miRNAs were hsa-miR-373, hsa-miR-675, hsa-miR-508, hsa-miR-1290 and hsa-miR−483-3p, while hsa-miR-105 and hsa-let-7f-1 were among the most down-regulated miRNAs compared to the normal salivary gland. Hsa-miR-106b, hsa-miR-142-5p, hsa-miR-622, hsa-miR-642, hsa-miR-574-3p and hsa-miR-486 were significantly differentially expressed (<i>p</i> &lt; 0.05) when each neoplasm and normal glandular tissue were compared.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study described the global molecular signature of miRNAs in benign and malignant salivary gland neoplasms and provided preliminary evidence of miRNA dysregulation, suggesting a potential association between specific miRNA signatures and molecular pathways implicated in tumour progression.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 8","pages":"906-916"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pg-Induced ATR Activation Promotes ESCC Progression via M2 TAM Polarization","authors":"Yanhua Sun,&nbsp;Lin Yang,&nbsp;Xiaodong Wei","doi":"10.1111/jop.70157","DOIUrl":"10.1111/jop.70157","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Background&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Emerging evidence suggests that oral pathogens may contribute to the development of systemic malignancies. \u0000 &lt;i&gt;Porphyromonas gingivalis&lt;/i&gt;\u0000 (Pg), a major periodontal pathogen, has been implicated in several cancers including esophageal squamous cell carcinoma (ESCC). However, the molecular mechanisms underlying this association remain unclear.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Objective&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;This study aimed to investigate the impact of \u0000 &lt;i&gt;Porphyromonas gingivalis&lt;/i&gt;\u0000 (Pg) on the growth of esophageal squamous cell carcinoma (ESCC) and its potential mechanisms.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;THP-1 cells were differentiated into M0 macrophages with PMA and divided into control group, Pg group, and Pg + siATR group. THP-1 cells were divided into three groups: control group, Pg group, and Pg + siATR group. The control and Pg groups were transfected with siNC, while the Pg + siATR group was transfected with siATR. After transfection, the Pg group and Pg + siATR group were incubated with 200 MOI pg. The control group was cultured normally. Cells and supernatants were collected, and macrophage polarization status was detected with qRT-PCR, Western blot, and flow cytometry. Macrophages treated differently were co-cultured with human esophageal squamous cell carcinoma cell line KYSE150. The proliferation, invasion, and apoptosis of KYSE150 were examined. KYSE150+shATR and KYSE150+shNC cell lines were constructed with a lentivirus system. Thirty male BALB/c mice aged 6–8 weeks were randomly divided into control group, pg group, and pg+shATR group, with 10 mice in each group. For the Pg and Pg+shATR groups, 200 μL Pg (1 × 10&lt;sup&gt;7&lt;/sup&gt; CFU/mouse) was applied to the mandibular molars of mice four times a week for 1 month. The control group was treated with the vehicle (CMC) only. After 3 weeks of bacterial colonization, KYSE150+shATR cells (1 × 10&lt;sup&gt;6&lt;/sup&gt; cells/mouse) were inoculated into the right axilla of mice in the Pg+shATR group, while KYSE150 + shNC (1 × 10&lt;sup&gt;6&lt;/sup&gt; cells/mouse) were inoculated into the right axilla of mice in the control and Pg groups. Tumor volume was measured with calipers every 7 days. After 4 weeks of experimentation, D-luciferin potassium salt was injected intraperitoneally at 150 mg/kg, and bioluminescence imaging was performed after sodium pentobarbital anesthesia. The mice were euthanized post-imaging, and macrophage polarization in tumor tissues was examined with qRT-PCR, histopathological examination, and Western blot.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;In this study, we fou","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 8","pages":"917-925"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148162942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Aurora Kinase A Prevents Malignant Transformation in Oral Leukoplakia.","authors":"Fengying Yin, Mengjun Li, Yi Xu, Chuan Xin, Yining Li, Qianming Chen","doi":"10.1111/jop.70177","DOIUrl":"https://doi.org/10.1111/jop.70177","url":null,"abstract":"<p><strong>Background: </strong>Aurora Kinase A (AURKA), a highly conserved and potent kinase, is frequently overexpressed in diverse cancers. However, its role in the malignant transformation (MT) of oral mucosa remains poorly understood. This study investigated the expression pattern of AURKA and evaluated the therapeutic potential of its inhibitor in oral leukoplakia (OLK), the most representative oral potentially malignant disorder.</p><p><strong>Methods: </strong>Immunohistochemistry was performed on clinical samples (21 normal controls, 27 OLK, 23 MT of OLK) to examine AURKA expression and its correlation with clinicopathological characteristics. The inhibitory effect of targeting AURKA on tumor proliferation was verified in vitro using two oral squamous cell carcinoma (OSCC) cell lines. Additionally, the chemopreventive effect of the AURKA inhibitor Alisertib on MT was assessed using a 4-nitroquinoline oxide (4NQO)-induced mouse OLK model.</p><p><strong>Results: </strong>AURKA expression levels positively correlated with the malignant progression of OLK. Significantly higher AURKA expression percentages were observed in non-homogeneous lesions (10.39% ± 4.28% vs. 3.53% ± 4.22% homogeneous, p < 0.001), lesions ≥ 2 cm² (11.19% ± 4.55% vs. 4.69% ± 4.36% < 2 cm², p < 0.01), and lesions with high-risk dysplasia (12.10% ± 4.04% vs. 4.59% ± 4.12% low-risk dysplasia, p < 0.001). Targeting AURKA significantly inhibited the proliferation of OSCC cells in vitro. Furthermore, Alisertib treatment effectively suppressed oral mucosa carcinogenesis in the 4NQO model.</p><p><strong>Conclusion: </strong>Increased AURKA expression may represent an early molecular event in OLK carcinogenesis, and targeting AURKA with alisertib is a candidate for potential applications in the management of OLK.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148864733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors of Lesion Detection in a 12-Year Oral Cancer Screening Program: A Cohort Study in Northern Portugal","authors":"Miguel Campos-Lopes,&nbsp;Fernanda Estevinho,&nbsp;Margarida Gouveia,&nbsp;Paulo Subtil,&nbsp;David Alfaiate,&nbsp;Paulo Nogueira,&nbsp;Paulo Sousa,&nbsp;João Leite-Moreira","doi":"10.1111/jop.70162","DOIUrl":"10.1111/jop.70162","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Oral cancer is a significant global public health issue, with high morbidity and mortality often linked to late diagnosis. This study evaluated a 12-year oral cancer screening program in northern Portugal to characterize its implementation and outcomes, identifying predictors of lesion detection. The program's outcomes were analyzed to assess its feasibility and contribution to early detection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A retrospective analysis of screening program data from 2012 to 2024 was conducted. Participants were recruited through community outreach and selection of high-risk groups in primary care settings. A standardized oral/oropharyngeal examination protocol was used to classify lesions as benign, suspicious, or malignant based on clinical examination. Demographic data and risk factors were recorded, and individuals with suspicious or malignant lesions were referred for specialized evaluation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 10 433 participants were screened (median age 63 years; 64% female). Oral lesions were detected in 16.7%, with 6.1% classified as suspicious and 0.2% malignant. Current smoking (OR = 1.65; <i>p</i> &lt; 0.001), former smoking (OR = 1.27; <i>p</i> = 0.010), and previous oncological disease (OR = 1.74; <i>p</i> &lt; 0.001) were associated with lesion detection on multivariable logistic regression. Overall, 16.4% of participants were referred for specialized consultation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This large-scale screening program successfully reached a broad population, identifying a substantial number of potentially malignant lesions. The association with known risk factors supports the need for targeted screening strategies. Further research should integrate diagnostic confirmation, evaluate long-term patient outcomes, and assess cost-effectiveness to refine oral cancer screening policies and healthcare resource allocation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 8","pages":"966-975"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148302069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic Value of Bcl-xL in Head and Neck Squamous Cell Carcinoma: A Systematic Review and Meta-Analysis","authors":"L. Leinonen,&nbsp;R. Tiikkaja,&nbsp;M. Juola,&nbsp;P. Åström,&nbsp;T. Salo,&nbsp;K. Juurikka,&nbsp;K. Korelin","doi":"10.1111/jop.70155","DOIUrl":"10.1111/jop.70155","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Head and neck squamous cell carcinoma is a heterogeneous disease with poor survival outcomes. Bcl-xL regulates tumor cell survival and apoptosis and has been associated with metastasis and poor prognosis, although its overall role remains undeciphered.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We systematically evaluated the prognostic value of Bcl-xL in head and neck squamous cell carcinoma following PRISMA 2020 guidelines. We identified 2241 reports from seven databases, of which 20 original studies with overall good quality (REMARK) and low bias (QUIPS) were included.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>An association between Bcl-xL levels and survival was rarely observed. However, high Bcl-xL levels correlated with increased risk of lymph node metastasis in 40% of studies. A study on oral tongue cancer identified a significant correlation between high Bcl-xL levels and decreased survival and lymph node metastasis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Current evidence does not support Bcl-xL as a general prognostic marker in head and neck squamous cell carcinoma. Nevertheless, Bcl-xL may have prognostic relevance in oral tongue cancer if further studies confirm the original finding.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 8","pages":"885-894"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148239585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of the EP300/Notch Signaling Pathway Regulates Proliferation and Apoptosis in Oral Squamous Cell Carcinoma","authors":"Honglan Wang,&nbsp;Yanzhen Fu,&nbsp;Gaoren Lin,&nbsp;Dandan Zhang,&nbsp;Xiaoxu Nan,&nbsp;Jiaming Liu,&nbsp;Xuelin Mou,&nbsp;Changyue Liu,&nbsp;Yuxuan Fan,&nbsp;Tong Zheng,&nbsp;Ying Liu","doi":"10.1111/jop.70159","DOIUrl":"10.1111/jop.70159","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Oral squamous cell carcinoma (OSCC) is highly recurrent and metastatic; EP300 drives tumorigenesis, but its mechanism is unclear.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>EP300 expression was profiled in OSCC via databases, RT-PCR, and Western blot. Knockdown effects on proliferation (CCK-8, colony), cell cycle, and apoptosis (flow cytometry) were measured. EP300–Notch interplay was probed with Valproic acid, a Notch signaling activator (VPA) rescue assays.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our experimental results showed that EP300 was upregulated in OSCC Cell Lines. In addition, bioinformatics analysis showed that EP300 upregulation was significantly associated with poor prognosis of OSCC. Prior research and bioinformatics analyses have demonstrated a close relationship between EP300 and the activation of the notch signaling pathway in OSCC. After EP300 knockdown in OSCC cells treated with VPA, our results indicated that VPA could partially reverse the effects of EP300 knockdown on cell proliferation, cell cycle, apoptosis, and EMT processes in OSCC Cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>In this study, we observed that EP300 knockdown suppressed the Notch signaling pathway, consequently inhibiting OSCC cell proliferation, the cell cycle, and EMT while also promoting apoptosis. These findings suggest that EP300 is crucial for OSCC cell growth and development.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 8","pages":"953-965"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148258480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implications of ADP-Ribosylation Factor-Like Protein 4C (ARL4C) in Human Neoplasia With Special Emphasis on Ameloblastoma: A Scoping Review","authors":"Muhammad Nazim Aiman Azhari,&nbsp;Chuey Chuan Tan,&nbsp;Lee Fah Yap,&nbsp;Siti Amalina Inche Zainal Abidin,&nbsp;Yet Ching Goh,&nbsp;Wanninayake Mudiyanselage Tilakaratne","doi":"10.1111/jop.70154","DOIUrl":"10.1111/jop.70154","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>ADP-ribosylation factor-like protein 4C (ARL4C), a member of the ARF small GTP-binding protein subfamily, has emerged from its role as an epithelial growth regulator to become a central molecule in cancer biology. This scoping review was conducted according to the PRISMA-ScR framework, systematically maps literature from PubMed, Scopus and Web of Science up to December 2024 to synthesise evidence on ARL4C's regulatory mechanisms and its implications in tumourigenesis and relevance to ameloblastoma.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Findings</h3>\u0000 \u0000 <p>Originally recognised for its role in tissue morphogenesis, ARL4C is now known to drive cancer cell proliferation, migration and invasion when dysregulated. Its expression is modulated by major signalling cascades including Wnt/MAPK, PI3K/AKT and TGF-β, as well as epigenetic factors. While primarily acting as an oncogenic driver, ARL4C exhibits context-dependent roles, occasionally functioning as a tumour suppressor. In ameloblastoma, a locally aggressive odontogenic tumour, ARL4C expression correlates with invasion and bone resorption. Notably, this influence appears independent of BRAF mutation status, suggesting a unique pathological role in this setting.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>ARL4C stands at the intersection of fundamental cell biology and clinical oncology. It holds promise as a biomarker for prognosis and recurrence and as a potential therapeutic target. However, its dual nature highlights the complexity of cancer signalling networks. Future research involving larger patient cohorts is essential to validate ARL4C's utility in precision oncology, particularly for the management of ameloblastoma.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 8","pages":"873-884"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Epithelial Dysplasia Information Needs Questionnaire (ODIN-Q): Responsiveness and Insights Into Patient Education","authors":"Waleed Alamoudi,&nbsp;Abdullah Alsoghier,&nbsp;Richeal Ni Riordain,&nbsp;Stefano Fedele,&nbsp;Stephen Porter","doi":"10.1111/jop.70160","DOIUrl":"10.1111/jop.70160","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The Oral Epithelial Dysplasia Informational Needs Questionnaire (ODIN-Q) was developed to assess the informational needs of patients with oral epithelial dysplasia (OED), a precancerous disorder associated with an increased risk of malignant transformation. This study aimed to evaluate the responsiveness of the ODIN-Q following an educational intervention using a written patient information leaflet about OED.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A prospective pre-post observational study was conducted at the Oral Medicine Unit at University College London Hospitals, between March 2023 and March 2025. Fifty patients with histologically confirmed OED completed the previously validated ODIN-Q before and after reading the leaflet. Differences between pre- and post-reading ODIN-Q scores were analysed using descriptive statistics and Cohen's <i>d</i> to determine effect sizes and responsiveness.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Fifty participants (29 females, 21 males; mean age = 65 years) were included in the analysis. Overall ODIN-Q scores increased from 2.44 to 2.71, with a small overall effect size (<i>d</i> = 0.30; 95% CI: 0.01–0.59). The highest responsiveness was observed in the medical system and access to information domain (+0.42; +19%; <i>d</i> = 0.49; 95% CI: 0.19–0.79), followed by psychosocial aspects (+0.29; +12%; <i>d</i> = 0.38; 95% CI: 0.09–0.67) and physical aspects (+0.28; +11%; <i>d</i> = 0.36; 95% CI: 0.07–0.65). Other domains showed negligible to small responsiveness (investigative tests: <i>d</i> = 0.11; 95% CI: −0.18–0.40).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The ODIN-Q demonstrates preliminary evidence of responsiveness to changes in patients' informational needs following educational interventions, supporting its potential use as a multidomain measure for evaluating and guiding patient-centred education in OED.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"55 8","pages":"946-952"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jop.70160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148239646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}