Journal of neurophysiology最新文献

{"title":"Acute pain impairs retention of locomotor learning, regardless of the context of retention testing.","authors":"Samuel R Jackson, Ryan T Pohlig, Susanne M Morton","doi":"10.1152/jn.00502.2024","DOIUrl":"10.1152/jn.00502.2024","url":null,"abstract":"<p><p>Our group and others have shown that the presence of an acute painful stimulus may interfere with retention of motor learning. Conversely, other evidence suggests this effect may not be truly due to pain, but due to a change in context when testing retention, i.e., testing retention in a nonpainful context when learning occurred in a painful context. Yet to our knowledge, no study has directly compared the retention of learning acquired under painful conditions with versus without a context change. To answer this question, we tested 30 young, healthy adults on a locomotor learning and retention paradigm. All participants walked on a treadmill with a monitor displaying distorted real-time visual feedback of step lengths to induce learning of an asymmetric stepping pattern. Retention was assessed 24 h later. Participants were randomized into one of three groups: one received no intervention; one received a painful stimulus during learning on <i>day 1</i> only; and one received the same painful stimulus during both learning on <i>day 1</i> and retention testing on <i>day 2</i>. Pain was induced by applying a combination of topical capsaicin cream and superficial heat to the skin of one leg. We found that while all groups successfully learned the asymmetric pattern, retention was reduced in both groups that experienced pain during learning, regardless of the pain context during retention testing. These findings indicate that pain experienced during the acquisition of a motor skill has a unique and deleterious effect on retention of that motor skill, which could negatively impact rehabilitation efforts.<b>NEW & NOTEWORTHY</b> Here, we show that acute pain experienced during locomotor learning reduces its 24-h retention regardless of the context in which retention is tested. These findings indicate that pain has a deleterious effect on the retention of newly acquired motor skills, possibly impacting the efficacy of motor learning-based rehabilitation interventions for people with painful conditions.</p>","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":"1807-1814"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional state correlates to survival and prognosis in children with brain tumor resection.","authors":"Yao Chen, Xin Hu, Ting Fan, Yongde Zhou, Cuiping Yu, Jian Ding, Jianfeng Yu, Ying Zhao, Nan Li, Baoguo Wang","doi":"10.1152/jn.00112.2025","DOIUrl":"10.1152/jn.00112.2025","url":null,"abstract":"<p><p>Pediatric brain tumors are the leading solid tumors in children. This study examines the critical factors influencing survival outcomes in pediatric patients undergoing brain tumor resection. We retrospectively analyzed 594 pediatric cases, collecting data on demographics, tumor characteristics, preoperative Glasgow Coma Scale (GCS), and American Society of Anesthesiologists (ASA) scores. Nutritional status was assessed using the STRONGkids tool. Survival analysis involved Kaplan-Meier estimates and Cox proportional hazards models. In regard to short-term prognosis, high nutritional risk was linked to prolonged hospital and ICU stays and more postoperative complications for all the cases. Considering the disease-free survival for malignant brain tumor cases, medium nutritional risk was associated with better survival compared with high nutritional risk (<i>P</i> < 0.001). Smaller tumor sizes and higher preoperative GCS scores correlated with improved survival rates (<i>P</i> = 0.005 and <i>P</i> < 0.001, respectively). Higher levels of preoperative albumin and prealbumin significantly increased survival (<i>P</i> < 0.001). ROC curve analysis identified optimal cutoffs for albumin (40.34 g/L) and prealbumin (192.1 mg/L) with corresponding sensitivities of 60.02% and 62.24%, and specificities of 85.06% and 73.28%, respectively. Larger tumor size, poor preoperative nutritional and neurological status, and suboptimal preoperative biochemical markers were significant predictors of increased mortality or recurrence risk. Preoperative nutritional assessment is crucial in pediatric brain tumor patients. Nutritional status, tumor size, and specific preoperative biochemical markers are vital for predicting disease-free survival outcomes. These findings highlight the need for integrating comprehensive preoperative evaluations into clinical protocols to enhance patient management and survival rates.<b>NEW & NOTEWORTHY</b> This is the first large-scale study to analyze the relationship between nutritional status and both short-term and relatively long-term outcomes in pediatric brain tumor patients.</p>","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":"1641-1648"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulated ischemia in live cerebral slices is mimicked by opening the Na⁺/K⁺ pump: clues to the generation of spreading depolarization.","authors":"Danielle Kim, Peter Gagolewicz, Sydney McQueen, Hannah Latour, Kaitlyn Tresidder, Cathryn R Jarvis, R David Andrew","doi":"10.1152/jn.00429.2024","DOIUrl":"10.1152/jn.00429.2024","url":null,"abstract":"<p><p>The gray matter of the higher brain undergoes spreading depolarization (SD) in response to the increased metabolic demand of ischemia, promoting acute neuronal injury and death following stroke, traumatic brain injury, or sudden cardiac arrest. The mechanism linking ischemic failure of the Na⁺/K⁺ ATPase (NKA) pump to the immediate onset of a large inward current driving SD has remained a mystery because blockade of conventional ion channels does not prevent SD nor ischemic neuron death. The marine poison palytoxin (PLTX) specifically binds the NKA at picomolar concentrations, converting this transporter to an open cationic channel, causing sudden neuronal Na⁺ influx and K⁺ efflux. This pump failure, together with induction of a strong inward current, should evoke SD-like activity in gray matter. Indeed, 1-10 nM PLTX applied to live coronal brain slices of rodents induces a propagating depolarization remarkably like SD induced by oxygen/glucose deprivation (OGD). This PLTX depolarization (PD) mimicked other effects of OGD. In the neocortex, as an elevated light transmittance (LT) front passed by an extracellular pipette, a distinct negative DC shift indicated mass cell depolarization, whether induced by bath OGD or PLTX. Either treatment induced strong SD-like responses in the same higher or lower brain regions. Furthermore, we imaged identical real-time OGD-SD or PD effects upon live pyramidal neurons using 2-photon microscopy. Taken together, these findings support our proposal that an endogenous PLTX-like molecule may open the NKA to conduct Na⁺ influx/K⁺ efflux, thereby driving SD and, in its wake, ensuing neuronal damage.<b>NEW & NOTEWORTHY</b> With stroke, traumatic brain injury, or sudden cardiac arrest, there is no therapeutic drug to aid brain recovery. Within 2 min of severe ischemia, a wave of spreading depolarization (SD) propagates through affected gray matter. More SDs arise over hours, expanding the injury. This period represents a therapeutic window to inhibit recurring SD and reduce neuronal damage, but we do not understand the underlying molecular sequence. Here, we argue for a novel molecule to target.</p>","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":"1649-1664"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frontal and temporo-parietal changes in delta and alpha power accompany stress-induced vasoconstriction and blood pressure response.","authors":"Joe Braun, Mariya Patel, Will Woods, Charlotte Keatch, Tatiana Kameneva, Elisabeth Lambert","doi":"10.1152/jn.00618.2024","DOIUrl":"10.1152/jn.00618.2024","url":null,"abstract":"<p><p>Exaggerated blood pressure and vasoconstriction responses during acute mental stress are prospectively associated with an increased risk of hypertension, arrhythmia, and vascular dysfunction. This study assessed electrophysiological brain power and cardiovascular response to acute psychological stress during concurrent recordings of magnetoencephalography, muscle sympathetic nerve activity, and blood pressure in 29 healthy participants. Brain power was filtered through delta (1-4 Hz), theta (4-8 Hz), alpha (8-13 Hz), beta (13-30 Hz), low gamma (30-80 Hz), and high gamma (80-120 Hz) bands. Stress induced significant increases in systolic blood pressure (SBP), 5.2 ± 5.6 mmHg, heart rate, 4.1 ± 4.8 beats/min, and sympathetic nerve activity, 4.0 ± 8.0 bursts per minute (means ± SD, <i>P</i> < 5). Whole head and region of interest analyses showed stress induced significant increases in delta power in the right medial frontal cortex, anterior cingulate, and amygdala. Alternatively, there were significant decreases in alpha power in the left and right precuneus, posterior cingulate, hippocampus, and in the right thalamus and insula. Correlation analyses revealed that reduced alpha power in the right insula was correlated with SBP (<i>r</i> = 0.446). Moreover, reduced alpha power in the left amygdala was correlated with SBP (<i>r</i> = 0.392), anxiety, and depression inventory scores, <i>P</i> < 0.01. The reduced alpha power in the insula and amygdala was pronounced in participants displaying heightened sympathetic nerve reactivity to stress. Similarly, participants who displayed higher mean SBP reactivity (of 19 mmHg) to stress showed reduced alpha power in the precuneus, hippocampus, and amygdala. Our results suggest that regionalized and frequency-specific brain power is involved in neurogenic blood pressure regulation in normotensive individuals.<b>NEW & NOTEWORTHY</b> This study illustrates a correlation between electrophysiological brain power, sympathetic nerve activity, and blood pressure in response to stress. Notable mental stress-induced changes were observed, including an increase in delta power in the frontal regions and a decrease in alpha power in the temporal and parietal regions. The diminished alpha power in the insula, amygdala, precuneus, and hippocampus was particularly significant among individuals exhibiting greater vasoconstrictor activity and a higher increase in systolic blood pressure.</p>","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":"1815-1827"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interneurons exhibit attenuated ectopic action potential firing in a severe neurodevelopmental disorder.","authors":"Sophie F Hill, Sophie R Liebergall, Eric R Wengert, Ethan M Goldberg, Brian B Theyel","doi":"10.1152/jn.00133.2025","DOIUrl":"10.1152/jn.00133.2025","url":null,"abstract":"<p><p>Dravet syndrome (DS) is a severe neurodevelopmental disorder associated with treatment-resistant epilepsy and features of autism spectrum disorder due to loss of the voltage-gated sodium channel subunit Nav1.1. Recent work suggests that a pathogenic mechanism of DS is impaired action potential propagation along axons of cerebral cortex parvalbumin-positive fast-spiking GABAergic interneurons (PVINs). Here, we investigated another aspect of axonal physiology: action potentials generated in the distal axon, known as \"ectopic\" action potentials (EAPs). We hypothesized that EAP frequency could be a proxy for the excitability of the distal axon and that EAPs would be attenuated in neocortical layer 2/3 PVINs from DS mice due to axonal dysfunction. We identified reduced EAP generation in DS PVINs at both <i>postnatal day</i> (P)18-21 and P35-56 and a complete absence of barrage (repetitive EAP) firing. This is the first evidence of impaired EAP firing in a disease model.<b>NEW & NOTEWORTHY</b> Dravet syndrome (DS) is a severe form of epilepsy primarily caused by reduced excitability of inhibitory neurons. Our research identifies a new abnormality in DS mice: reduced ectopic action potentials (EAPs). We have previously shown that EAPs are engaged after increased excitability, manifesting in most parvalbumin-expressing interneurons (PVINs) as a high-frequency train of persistent action potentials. Our work represents the first evidence linking a deficiency in EAP generation-an underexplored intrinsic property-with any neuropathology.</p>","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":"1692-1698"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reverse correlation of natural statistics for ecologically relevant characterization of human perceptual templates.","authors":"Lorenzo Landolfi, Peter Neri","doi":"10.1152/jn.00059.2024","DOIUrl":"10.1152/jn.00059.2024","url":null,"abstract":"<p><p>Psychophysical reverse correlation is an established technique for characterizing perceptual templates. Its application is best suited to a scenario in which <i>1</i>) the human observer operates as a template matcher, and <i>2</i>) the perceptual system is probed using radially symmetric noise, such as Gaussian white noise. When both conditions apply, the resulting estimate of the perceptual template directly reflects the actual template engaged by observers. However, when either condition fails, template estimates can be highly distorted to the point of becoming uninterpretable. This limitation is particularly pertinent when ecological relevance is under consideration because natural signals are clearly nothing like white noise. Template distortions associated with natural statistics may be corrected using a number of methods, many of which have been tested in single neurons, but none of which has been tested in human observers. We studied the applicability (or lack thereof) of five such methods to multiple experimental conditions under which the human visual system approaches a template matcher to different degrees of approximation. We find that methods based on minimizing/maximizing loss/information, such as logistic regression and maximally informative dimensions, outperform other approaches under the conditions of our experiments, and therefore represent promising tools for the characterization of human perceptual templates under ecologically relevant conditions. However, we also identify plausible scenarios under which those same approaches produce misleading outcomes, urging caution when interpreting results from those and related methods.<b>NEW & NOTEWORTHY</b> Reverse correlation is the method of choice for estimating neuronal/perceptual receptive fields, however, its applicability to natural behavior is hampered by the highly structured statistics of natural scenes. Although contemporary techniques for incorporating natural statistics have proven successful in neuronal settings, their applicability to psychophysical settings is unknown. We demonstrate that those techniques are indeed applicable to human observers, but with some important caveats that, if ignored, may lead to gross misinterpretations of the perceptual process.</p>","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":"1717-1739"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neural and psychoacoustic insights into amplitude modulation processing and its link with speech perception in noise.","authors":"Clémence Basire, Irene Lorenzini, Laurianne Cabrera","doi":"10.1152/jn.00007.2025","DOIUrl":"10.1152/jn.00007.2025","url":null,"abstract":"<p><p>Temporal modulations, especially amplitude modulations (AMs), play a fundamental role in speech perception in noisy environments, as highlighted by psychoacoustic research. However, the mechanisms underlying AM processing and its contribution to speech perception in noise remain unclear. The present study combined both behavioral psychophysics and electroencephalography (EEG) measures to investigate AM processing and its relationship to the perception of consonants in noise for young adults with normal hearing. Participants completed psychoacoustic measures of AM sensitivity, i.e., detection thresholds for AM fluctuations at 8 Hz, and susceptibility to AM masking (the effect on AM detection thresholds of interfering AM fluctuations carried by a masker) at 8 Hz. In addition, participants underwent an EEG recording measuring the neural AM following response (AMFR, or envelope following response) for AM tones modulated at 8 or 40 Hz presented without masking. Finally, they completed a consonant identification task in a stationary speech-shaped noise. Results showed a significant positive correlation between the signal-to-noise ratio of the AMFR recorded at 8 Hz and AM masking effects measured at the same rate. This finding is discussed in terms of both reduced selectivity of AM filters and elevated internal noise. Finally, no relationship between AM measures and consonant-in-noise perception was observed, suggesting that the mechanisms involved in AM processing may not fully capture the ability of adults with normal hearing to identify consonants in noisy backgrounds.<b>NEW & NOTEWORTHY</b> Results showed a significant correlation between the amplitude modulation (AM) following response (AMFR) and AM masking effect. Participants who are more susceptible to AM masking exhibit higher neural tracking of AM at the same fluctuation rate (8 Hz). This result might be explained both in terms of reduced selectivity of AM filters and elevated internal noise. Our results also showed no relationship between AM measures and consonant-in-noise perception, indicating that AM sensitivity mechanisms do not fully explain speech-in-noise perception skills in young normal-hearing adults.</p>","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":"1828-1835"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological modulation of the M-current shapes locomotor function in developing zebrafish.","authors":"Stephanie F Gaudreau, Tuan V Bui","doi":"10.1152/jn.00003.2025","DOIUrl":"10.1152/jn.00003.2025","url":null,"abstract":"<p><p>The M-current (<i>I</i>_M) is a noninactivating potassium current that has been implicated in the control of locomotion in mammals, where it was shown to shape the rhythm governing locomotor movements. We tested whether <i>I</i>_M might also be involved in the control of locomotor movements in developing zebrafish. Specifically, we investigated the involvement of <i>I</i>_M in the execution of escape responses and swimming movement of zebrafish aged 4-5 days postfertilization (dpf) using XE-991 and ICA-069673, a pharmacological blocker and enhancer of <i>I</i>_M, respectively. We found that <i>I</i>_M may influence the number and type of swim bouts in an escape response but not the duration of the first bout nor the tail beat frequency. Enhancing <i>I</i>_M reduced the distance swam with slow or fast swimming maneuvers by freely behaving larval zebrafish. We then studied whether <i>I</i>_M is involved in locomotor output by spinal circuits using various approaches to induce motor activity in spinalized 4-5 dpf zebrafish. We found that while modulating <i>I</i>_M during <i>N-</i>methyl-d-aspartic acid (NMDA)-evoked swimming activity had negligible effects, modulating <i>I</i>_M during swimming activity evoked by a generalized depolarization of the spinal cord affected specific swimming parameters. In particular, XE-991 and ICA-069673 had opposite effects on overall spiking activity, swimming episode frequency and duration, and the number of bursts within each episode. In summary, <i>I</i>_M was found to be involved in certain facets of escape response and swimming in larval zebrafish, and some of this influence resides within the expression of this current in spinal circuits.<b>NEW & NOTEWORTHY</b> We demonstrate for the first time a role of the M-current in specific parameters of escape responses and swimming in larval zebrafish. Experiments conducted in isolated spinal preparations point to the presence of the M-current in spinal circuits of larval zebrafish.</p>","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":"1795-1806"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of the Aβ receptor that controls the voltage-gated sodium channel activity: unraveling mechanisms underlying neuronal hyperexcitability.","authors":"Mitsuyoshi Luke Saito, Tsutomu Sasaki, Mariko Ruth Saito","doi":"10.1152/jn.00530.2024","DOIUrl":"10.1152/jn.00530.2024","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is characterized by a gradual decline in memory and cognitive abilities, often accompanied by personality changes and impairments in motor functions. Increased neuronal activity in AD patients is associated with the symptoms of the disease, suggesting a link between hyperactivity and cognitive decline. In particular, amyloid beta peptides (Aβs), which are implicated in AD, have been found to enhance voltage-gated sodium channels (VGSCs), crucial for generating nerve impulses. However, the exact mechanisms underlying this interaction remain poorly understood. Therefore, it is crucial to identify the membrane receptor that binds to Aβ and regulates VGSC activity. In this report, we employed the patch-clamp method to monitor alterations in VGSCs induced by Aβ. Through gene silencing and antibody treatment, we determined that the receptor responsible for regulating VGSCs corresponds to the type I taste receptor (T1R2/T1R3). Our discovery not only advances the understanding of Aβ's physiological role but also opens avenues for developing molecules that can inhibit or alter Aβ binding, potentially regulating neuronal hyperactivity in AD.<b>NEW & NOTEWORTHY</b> Alzheimer's disease (AD) is marked by memory loss and cognitive decline, with neuronal hyperactivity linked to amyloid beta peptides (Aβs) that enhance sodium channels. Using patch-clamp techniques, we determined that the receptor for Aβ corresponds to the type I taste receptor (T1R2/T1R3). This discovery reveals Aβ's physiological roles and offers a new molecular target for developing therapies to inhibit or modify Aβ binding, potentially regulating neurohyperactivity in AD.</p>","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":"1861-1885"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effects of dopamine agonists on pain-responsive neurons in the central nucleus of the amygdala.","authors":"Robert J Heuermann, Robert W Gereau","doi":"10.1152/jn.00135.2025","DOIUrl":"https://doi.org/10.1152/jn.00135.2025","url":null,"abstract":"<p><p>The central nucleus of the amygdala (CeA) is a heterogenous region of primarily GABAergic neurons that contributes to numerous behaviors, including fear learning, feeding, reward, and pain. Dopaminergic inputs to the CeA have been shown to regulate many of these behaviors, but how dopamine exerts these effects at the cellular level has not been well characterized. We used the Targeted Recombination in Active Populations (TRAP) mouse line to fluorescently label pain-responsive CeA neurons, and then targeted these cells for patch-clamp recordings in acute slices to test the effects of dopamine agonists. The D1 agonist SKF-38393 and D2 agonist quinpirole both had inhibitory effects, reducing the input resistance and evoked firing and increasing rheobase of labeled CeA neurons. Both agents also inhibited the NMDA component of excitatory postsynaptic currents (EPSCs) evoked by basolateral amygdala (BLA) stimulation, but did not affect the AMPA component. D1 activation, but not D2, also had a possible presynaptic effect, increasing the frequency of spontaneous EPSCs. These results provide new insights into how dopamine regulates activity within pain-responsive CeA networks.</p>","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}