Journal of neurophysiology最新文献

Divisive attenuation based on noisy sensorimotor predictions accounts for excess variability in self-touch. 基于噪声感觉运动预测的分裂衰减解释了自触摸的过度变异性。

IF 2.1 3区医学

Journal of neurophysiology Pub Date : 2025-06-18 DOI: 10.1152/jn.00055.2025

Nicola Valè, Ivan Tomic, Zahara Gironés, Daniel M Wolpert, Konstantina Kilteni, Paul M Bays

{"title":"Divisive attenuation based on noisy sensorimotor predictions accounts for excess variability in self-touch.","authors":"Nicola Valè, Ivan Tomic, Zahara Gironés, Daniel M Wolpert, Konstantina Kilteni, Paul M Bays","doi":"10.1152/jn.00055.2025","DOIUrl":"https://doi.org/10.1152/jn.00055.2025","url":null,"abstract":"When one part of the body exerts force on another, the resulting tactile sensation is perceived as weaker than when the same force is applied by an external agent. This phenomenon has been studied using a force matching task, in which observers are first exposed to an external force on a passive finger and then instructed to reproduce the sensation by directly pressing on the passive finger with a finger of the other hand: healthy participants consistently exceed the original force level. However, this exaggeration of the target force is not observed if the observer generates the matching force indirectly, by adjusting a joystick or slider that controls the force output of a motor. Here we present the first detailed computational account of the processes leading to exaggeration of target forces in the force matching task, incorporating attenuation of sensory signals based on motor predictions. The model elucidates previously unappreciated contributions of multiple sources of noise, including memory noise, in determining matching force output, and shows that quantifying attenuation as the discrepancy between direct and indirect self-generated forces isolates its predictive component. Our computational account makes the prediction that attenuated sensations will display greater trial-to-trial variability than unattenuated ones, because they incorporate additional noise from motor prediction. Quantitative model fitting of new and existing force matching data confirmed the prediction of excess variability in self-generated forces and provided evidence for a divisive rather than subtractive mechanism of attenuation, while highlighting its predictive nature.","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unpredictable movement-evoked pain alters cortical motor preparatory activity. 不可预测的运动引起的疼痛改变了皮质运动准备活动。

IF 2.1 3区医学

Journal of neurophysiology Pub Date : 2025-06-18 DOI: 10.1152/jn.00237.2025

Emmanuel Ogalo, Hannah J Ro, Lukas D Linde, Oscar Ortiz, Michael J Berger, John L K Kramer

{"title":"Unpredictable movement-evoked pain alters cortical motor preparatory activity.","authors":"Emmanuel Ogalo, Hannah J Ro, Lukas D Linde, Oscar Ortiz, Michael J Berger, John L K Kramer","doi":"10.1152/jn.00237.2025","DOIUrl":"https://doi.org/10.1152/jn.00237.2025","url":null,"abstract":"Although there is substantial evidence that central motor system function is altered both during and after pain removal, the effect of pain on motor preparation remains poorly understood. The present study used electroencephalography (EEG) to examine whether the predictability of movement-evoked pain modulates cortical preparatory activity, and if changes in cortical activity persist after the removal of pain. The movement-related cortical potential (MRCP), alpha frequency band (8-12 Hz) oscillatory activity and the vertex laser-evoked potential (N2P2) were evaluated in fifteen healthy adults (9 male, age = 30.3 ± 10.2 years). Under unpredictable pain expectancy, peak MRCP amplitude (p = 0.044), premovement alpha activity (p = 0.002), and N2P2 amplitude (p < 0.001) all significantly increased. These preparatory changes did not persist once pain was removed (MRCP: p = 1.0; alpha ERSP: p = 1.0 ). These findings suggest that unpredictable pain expectancy modulates motor preparation through top-down attentional mechanisms that serve to suppress distractor sensorimotor processing and enhance task-relevant neural activity to support optimal motor programming.","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unified Measures Quantifying Intensity and Similarity of Pain and Somatosensory Percepts. 量化疼痛和躯体感觉知觉的强度和相似性的统一措施。

IF 2.1 3区医学

Journal of neurophysiology Pub Date : 2025-06-16 DOI: 10.1152/jn.00031.2025

Eric J Earley, Malin Ramne, Johan Wessberg

{"title":"Unified Measures Quantifying Intensity and Similarity of Pain and Somatosensory Percepts.","authors":"Eric J Earley, Malin Ramne, Johan Wessberg","doi":"10.1152/jn.00031.2025","DOIUrl":"https://doi.org/10.1152/jn.00031.2025","url":null,"abstract":"Across somatosensory and pain literature, there exist several methods of characterizing the location and extent of perceived sensations, and quantifying how these sensory maps may differ. However, these measures of somatosensory intensity and similarity can give non-unique results, creating challenges in literature review and meta-analysis across different methods. In this paper, we propose novel and unifying measures to quantify the similarity and intensity of pain maps and somatosensory percepts. These measures are generalizable and can be applied to any application of somatosensory maps, and are usable with both discretized and free-hand drawings in both 2D and 3D representations. Somatosensory Percept Intensity (SPI) is inspired by Piper's Law, which describes the phenomenon of incomplete spatial summation wherein changes in pain area do not yield linearly proportional changes in perceived intensity. Somatosensory Percept Deviation (SPD) is derived from optimal transport theory, which quantifies differences between two probability distributions or somatosensory maps. Mathematical derivations for both measures are provided. The utility of these measures is demonstrated using data from two studies - one characterizing elicited somatosensory percepts, and one investigating neuropathic pain drawings. The proposed measures strongly agree with the validation studies, illustrating their potential as agnostic measures for characterizing somatosensory percepts in studies and meta-analyses. Ultimately, our work yields powerful unified measures for use in the fields of perception and pain, and may aid in improved pain characterization within healthcare, granting a better understanding of the needs and progression of patients experiencing pain.","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional segregation of cortical hand and speech areas by frequency detuning of an intrinsic motor rhythm. 由内在运动节奏的频率失谐引起的手皮层和言语区功能分离。

IF 2.1 3区医学

Journal of neurophysiology Pub Date : 2025-06-16 DOI: 10.1152/jn.00546.2024

Ioanna Anastasopoulou, Douglas O Cheyne, Blake W Johnson

{"title":"Functional segregation of cortical hand and speech areas by frequency detuning of an intrinsic motor rhythm.","authors":"Ioanna Anastasopoulou, Douglas O Cheyne, Blake W Johnson","doi":"10.1152/jn.00546.2024","DOIUrl":"https://doi.org/10.1152/jn.00546.2024","url":null,"abstract":"Many decades after Penfield's (1937) classic depiction of the motor homunculus, it remains unclear how spatially contiguous and interconnected representations within human sensorimotor cortex might separate their activities to achieve the directed and precise control of distinct body regions evident in activities as different as typing and speaking. One long-standing but relatively neglected explanation draws from models of simple physical systems (like swinging pendulums) to posit that small differences in the oscillatory properties of neuronal populations (termed \"frequency detuning\") can result in highly effective segregation of their activities and outputs. We tested this hypothesis by comparing the peak frequencies of beta-band (13-30 Hz) motor rhythms measured in a magnetoencephalographic neuroimaging study of finger and speech movements in a group of healthy adults and a group of typically developing children. Our results confirm a peak frequency task difference (speech movement versus hand movement) of about 1.5 Hz in the beta motor rhythms of both left and right hemispheres in adults. A comparable task difference was obtained in children for the left but not for the right hemisphere. These results provide novel support for the role of frequency detuning in the functional organisation of the brain and suggest that this mechanism should play a more prominent role in current models of bodily representations and their development within the sensorimotor cortex.","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Internal models in active self-motion estimation: role of inertial sensory cues. 主动自运动估计中的内部模型：惯性感觉线索的作用。

IF 2.1 3区医学

Journal of neurophysiology Pub Date : 2025-06-14 DOI: 10.1152/jn.00281.2024

Milou J L van Helvert, Luc P J Selen, Robert J van Beers, W Pieter Medendorp

{"title":"Internal models in active self-motion estimation: role of inertial sensory cues.","authors":"Milou J L van Helvert, Luc P J Selen, Robert J van Beers, W Pieter Medendorp","doi":"10.1152/jn.00281.2024","DOIUrl":"https://doi.org/10.1152/jn.00281.2024","url":null,"abstract":"Self-motion estimation is thought to depend on sensory information as well as on sensory predictions derived from motor output. In driving, the inertial motion cues (vestibular and somatosensory cues) can in principle be predicted based on the steering motor commands if an accurate internal model of the steering dynamics is available. Here, we used a closed-loop steering experiment to examine whether participants can build such an internal model of the steering dynamics. Participants steered a motion platform on which they were seated to align their body with a memorized visual target in complete darkness. We varied the gain between the steering wheel angle and the velocity of the motion platform across trials in three different ways: unpredictable (white noise), moderately predictable (random walk), or highly predictable (constant gain). We examined whether participants took the across-trial predictability of the gain into account to control their steering (internal model hypothesis), or whether they simply integrated the inertial feedback over time to estimate their travelled distance (path integration hypothesis). Results show that participants relied on the gain of the previous trial more when it followed a random walk across trials than when it varied unpredictably across trials. Furthermore, on interleaved trials with a large jump in the gain, participants made fast corrective responses, irrespective of gain predictability, showing they also relied on inertial feedback next to predictions. These findings suggest that the brain can construct an internal model of the steering dynamics to predict the inertial sensory consequences in driving and self-motion estimation.","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Odor encoding by fine-timescale spike synchronization patterns in the olfactory bulb. 嗅球中精细时间尺度刺突同步模式的气味编码。

IF 2.1 3区医学

Journal of neurophysiology Pub Date : 2025-06-14 DOI: 10.1152/jn.00340.2024

Jesse C Werth, Matthew Einhorn, Thomas A Cleland

{"title":"Odor encoding by fine-timescale spike synchronization patterns in the olfactory bulb.","authors":"Jesse C Werth, Matthew Einhorn, Thomas A Cleland","doi":"10.1152/jn.00340.2024","DOIUrl":"https://doi.org/10.1152/jn.00340.2024","url":null,"abstract":"In the mammalian olfactory bulb (OB), gamma oscillations in the local field potential are generated endogenously during odor sampling. Such oscillations arise from dynamical systems that generate organized periodic behavior in neural circuits, and correspond to spike timing constraints at millisecond timescales. While the cellular and network mechanisms of gamma oscillogenesis in the OB are reasonably well established, it remains unclear how these fine-timescale dynamics serve to represent odors. Are patterns of spike synchronization on the gamma timescale replicable and odor-specific? Does the transformation to a spike-timing metric embed additional signal processing computations? To address these questions, we used OB slice recordings to examine the spike timing dynamics evoked by \"fictive odorants\" generated via spatiotemporally patterned optogenetic stimulation of olfactory sensory neuron axonal arbors. We found that a small proportion of mitral/tufted cells phase-lock strongly to the fast oscillations evoked by fictive odorants, and exhibit tightly coupled spike-spike synchrony on the gamma timescale. Moreover, the specific population of synchronized neurons corresponded to the \"quality\", but not the \"concentration\" (intensity), of the fictive odorant presented, and was conserved across multiple presentations of the same fictive odorant. Given the established selectivity of piriform cortical pyramidal neurons for inputs synchronized on this timescale, we conclude that spike synchronization on a milliseconds timescale is a metric by which the OB encodes and exports afferent odor information in a concentration-invariant manner. As a corollary, mitral/tufted cell spikes that are not organized in time are unlikely to contribute meaningfully to the ensemble odor representation.","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of short-term dual action-simulation training combined with transcranial magnetic stimulation on corticospinal excitation and finger motor performance. 短期双动作模拟训练联合经颅磁刺激对皮质脊髓兴奋和手指运动表现的影响。

IF 2.1 3区医学

Journal of neurophysiology Pub Date : 2025-06-11 DOI: 10.1152/jn.00483.2024

Kazumasa Konishi, Shinya Suzuki, Tsuyoshi Nakajima, Hideto Sano, Yosuke Kawano, Takehiko Moroi, Takumi Takeuchi, Masahito Takahashi, Satoshi Shibuya, Yohei Nagaoka, Naobumi Hosogane, Yukari Ohki

{"title":"Effects of short-term dual action-simulation training combined with transcranial magnetic stimulation on corticospinal excitation and finger motor performance.","authors":"Kazumasa Konishi, Shinya Suzuki, Tsuyoshi Nakajima, Hideto Sano, Yosuke Kawano, Takehiko Moroi, Takumi Takeuchi, Masahito Takahashi, Satoshi Shibuya, Yohei Nagaoka, Naobumi Hosogane, Yukari Ohki","doi":"10.1152/jn.00483.2024","DOIUrl":"https://doi.org/10.1152/jn.00483.2024","url":null,"abstract":"Action-simulation training using action observation (AO), motor imagery (MI), or a combination of both (AOMI) may improve motor function in patients with neurological diseases. While multiple sessions over several days or weeks are necessary to produce neurophysiological and behavioral effects in patients, the aftereffects of a single session are crucial for achieving long-term outcomes. This study aimed to investigate whether a single session of dual action-simulation (AOMI) training combined with transcranial magnetic stimulation (TMS) induces plastic changes in corticospinal excitation over time and affects motor performance in healthy individuals. The results demonstrated that 20 min of AOMI + TMS training produced a sustained increase in the amplitudes of motor evoked potentials (MEPs), lasting for >30 min. Additionally, inter-individual variations in MEP amplitudes after AOMI + TMS could be predicted using MEP amplitude changes during training. The control experiments (MI + TMS, AO + TMS, and AOMI + TMSsham) used to clarify which combination of the training components promoted increased MEP amplitudes, did not induce significant plastic changes. Furthermore, participants who underwent AOMI + TMS showed improved finger motor performance after training, while the control participants did not. These findings suggest that a single session of dual action-simulation training combined with TMS enhances corticospinal transmission over time and improves finger motor performance in healthy individuals.","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased co-activation during clinical tests of spasticity is associated with increased co-activation during reactive balance control in cerebral palsy. 痉挛临床试验中增加的共激活与脑瘫反应性平衡控制中增加的共激活相关。

IF 2.1 3区医学

Journal of neurophysiology Pub Date : 2025-06-11 DOI: 10.1152/jn.00568.2024

Jente Willaert, Lena H Ting, Anja Van Campenhout, Kaat Desloovere, Friedl De Groote

{"title":"Increased co-activation during clinical tests of spasticity is associated with increased co-activation during reactive balance control in cerebral palsy.","authors":"Jente Willaert, Lena H Ting, Anja Van Campenhout, Kaat Desloovere, Friedl De Groote","doi":"10.1152/jn.00568.2024","DOIUrl":"https://doi.org/10.1152/jn.00568.2024","url":null,"abstract":"Joint hyper-resistance is a common symptom in cerebral palsy (CP). It is assessed by rotating the joint of a relaxed patient. Joint rotations also occur when perturbing functional movements. Therefore, joint hyper-resistance might contribute to reactive balance impairments in CP. Our aim was to investigate relationships between altered muscle responses to isolated joint rotations and perturbations of standing balance in children with CP. Twenty children with CP and twenty typically developing children participated in the study. During an instrumented spasticity assessment, the ankle was rotated as fast as possible from maximal plantarflexion towards maximal dorsiflexion. Standing balance was perturbed by backward support-surface translations and toe-up support-surface rotations. Gastrocnemius, soleus, and tibialis anterior electromyography was measured. We evaluated alterations in reciprocal pathways by plantarflexor-dorsiflexor co-activation and the neural response to stretch by average muscle activity. We evaluated the relation between muscle responses to ankle rotation and balance perturbations using linear mixed models. Co-activation during isolated joint rotations and perturbations of standing balance was correlated in CP but not in typically developing children. The neural response to stretch during isolated joint rotations and balance perturbations was not correlated. Our results suggest that increased co-activation, possibly due to reduced reciprocal inhibition, during isolated joint rotations might be a predictor of altered reactive balance control strategies in CP.","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Motor sequence learning elicits mu peak-specific corticospinal plasticity. 运动序列学习诱发了脑峰特异性皮质脊髓可塑性。

IF 2.1 3区医学

Journal of neurophysiology Pub Date : 2025-06-11 DOI: 10.1152/jn.00579.2024

Tharan Suresh, Fumiaki Iwane, Minsu Zhang, Margaret McElmurry, Muskan Manesiya, Michael V Freedberg, Sara J Hussain

{"title":"Motor sequence learning elicits mu peak-specific corticospinal plasticity.","authors":"Tharan Suresh, Fumiaki Iwane, Minsu Zhang, Margaret McElmurry, Muskan Manesiya, Michael V Freedberg, Sara J Hussain","doi":"10.1152/jn.00579.2024","DOIUrl":"10.1152/jn.00579.2024","url":null,"abstract":"Motor cortical (M1) transcranial magnetic stimulation (TMS) interventions increase corticospinal output and improve motor learning when delivered during sensorimotor mu rhythm trough but not peak phases, suggesting that the mechanisms supporting motor learning may be most active during mu trough phases. Based on these findings, we predicted that motor sequence learning-related corticospinal plasticity would be most evident when measured during mu trough phases. Healthy adults were assigned to either a sequence or no-sequence group. Participants in the sequence group practiced the implicit serial reaction time task (SRTT), which contained an embedded, repeating 12-item sequence. Participants in the no-sequence group practiced a version of the SRTT that contained no sequence. We measured mu phase-independent and mu phase-dependent MEP amplitudes using EEG-informed single-pulse TMS before, immediately after, and 30 minutes after the SRTT in both groups. All participants performed a retention test one hour after SRTT acquisition. In both groups, mu phase-independent MEP amplitudes increased following SRTT acquisition, but the pattern of mu phase-dependent MEP amplitude changes after SRTT acquisition differed between groups. Relative to the no-sequence group, the sequence group showed greater peak-specific MEP amplitude increases 30 minutes after SRTT acquisition. Further, the magnitude of these peak-specific MEP amplitude increases was negatively associated with the magnitude of sequence learning. Contrary to our original hypothesis, results revealed that motor sequence learning elicits peak-specific corticospinal plasticity. Findings provide first direct evidence that motor sequence learning recruits mu phase-dependent neurophysiological processes in the human brain.","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocide mixture (CMIT/MIT) induces neurotoxicity through the upregulation of the mitogen-activated protein kinases (MAPKs) signaling pathways. 生物杀灭剂混合物（CMIT/MIT）通过上调丝裂原活化蛋白激酶（MAPKs）信号通路诱导神经毒性。

IF 2.1 3区医学

Journal of neurophysiology Pub Date : 2025-06-11 DOI: 10.1152/jn.00104.2025

Francesco Molinari, Nicla Tranchida, Francesca Inferrera, Roberta Fusco, Caterina Faggio, Federica Impellitteri, Salvatore Cuzzocrea, Marika Cordaro, Rosanna Di Paola

{"title":"Biocide mixture (CMIT/MIT) induces neurotoxicity through the upregulation of the mitogen-activated protein kinases (MAPKs) signaling pathways.","authors":"Francesco Molinari, Nicla Tranchida, Francesca Inferrera, Roberta Fusco, Caterina Faggio, Federica Impellitteri, Salvatore Cuzzocrea, Marika Cordaro, Rosanna Di Paola","doi":"10.1152/jn.00104.2025","DOIUrl":"https://doi.org/10.1152/jn.00104.2025","url":null,"abstract":"The biocides 5-chloro-2-methyl-2h-isothiazolin-3-one and 2-methyl-2h-isothiazolin-3-one (CMIT/MIT) are widely used and can be found in many different types of water-soluble consumer products, such as shampoo, dentifrice, and germicide. Recent reports have suggested that it may be harmful to the skin and lungs. Although not known to be linked to pathogenic cellular and molecular pathways, it is a recognized risk factor for endangering public health. Therefore, the aim of this study was to examine the impact of CMIT/MIT (in 3:1 ratio) in SH-SY5Y human neuroblastoma cells. SHSY-5Y cells were exposed to different concentration (0, 12.5, 25 and 50 μM) of CMIT/MIT for 24 h. Cellular proliferation was considerably reduced in the MTT assay after CMIT/MIT exposure. Additionally, the results showed an increase in LDH release and lipid peroxidation and a decrease in physiological antioxidant defense. We also observed an activation of Nrf-2/HO-1 signaling pathway by western blot and qRT-PCR. Exposure to CMIT/MIT (in 3:1 ratio) also increased the release of pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α. Furthermore, in SHSY-5Y, CMIT/MIT (in 3:1 ratio) raised the levels of phosphorylated ERK1/2, phosphorylated p38, and phosphorylated JNK1/2 proteins. The activation of these pathways was strongly connected with the cell cycle-related genes p53 and p21 and the activation of apoptotic cascade. These results imply that the Nrf-2/HO-1, p38-JNK1/2-ERK1/2, Bax/Bcl-2 signaling pathways are responsible for inducing cellular damage and accelerating neuronal aging in response to CMIT/MIT (in 3:1 ratio) exposure.","PeriodicalId":16563,"journal":{"name":"Journal of neurophysiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0