Journal of Neurogastroenterology and Motility最新文献

{"title":"Natural History of Chronic Intestinal Pseudo-obstruction and Need for Palliative Care.","authors":"Kosuke Tanaka,&nbsp;Hidenori Ohkubo,&nbsp;Atsushi Yamamoto,&nbsp;Kota Takahashi,&nbsp;Yuki Kasai,&nbsp;Anna Ozaki,&nbsp;Michihiro Iwaki,&nbsp;Takashi Kobayashi,&nbsp;Tsutomu Yoshihara,&nbsp;Noboru Misawa,&nbsp;Akiko Fuyuki,&nbsp;Shingo Kato,&nbsp;Takuma Higurashi,&nbsp;Kunihiro Hosono,&nbsp;Masato Yoneda,&nbsp;Takeo Kurihashi,&nbsp;Masataka Taguri,&nbsp;Atsushi Nakajima,&nbsp;Kok-Ann Gwee,&nbsp;Takaomi Kessoku","doi":"10.5056/jnm22152","DOIUrl":"https://doi.org/10.5056/jnm22152","url":null,"abstract":"<p><strong>Background/aims: </strong>Natural history of chronic intestinal pseudo-obstruction (CIPO), a rare disease characterized by episodes of non-mechanical obstruction, is unclear in adults. This study evaluates the clinical course of CIPO and palliative care needs of patients.</p><p><strong>Methods: </strong>From October 2010 to September 2021, 74 patients who underwent cine MRI and had a definitive diagnosis of CIPO were prospectively included. We investigated disease etiology and outcomes, age at onset, nutritional status at consultation (body mass index and serum albumin), hydrogen breath test results, and total parenteral nutrition (TPN) during the disease course.</p><p><strong>Results: </strong>Forty-seven patients (64%) were women, with a mean age of 44 years at onset and 49 years at diagnosis. Primary CIPO was observed in 48 patients (65%). Secondary CIPO was observed in 26 cases (35%), of whom 18 (69%) had scleroderma. The mean body mass index, serum albumin level, and hydrogen breath test positivity rate were 17 kg/m², 3.8 mg/dL, and 60%, respectively. TPN and invasive decompression therapy were required by 23 (31%) and 18 (24%) patients, respectively. Intestinal sterilization was performed in 51 (69%) patients and was effective in 33 (65%); of these, 28 (85%) were taking metronidazole. Seven (9%) patients used opioids. There were 9 deaths (12%), including 5 (56%) from infection and 2 (22%) from suicide. Of the deaths, 6 (67%) and 4 (44%) underwent TPN management and decompression therapy, respectively. Fifty-one patients (69%) wanted palliative care.</p><p><strong>Conclusion: </strong>CIPO is a rare, severe, and under-recognized disease. Standardization of treatment strategies, including palliative care and psychiatric interventions, is desired.</p>","PeriodicalId":16543,"journal":{"name":"Journal of Neurogastroenterology and Motility","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/59/56/jnm-29-3-378.PMC10334206.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10269011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2022 Seoul Consensus on Clinical Practice Guidelines for Functional Constipation.","authors":"Young Sin Cho,&nbsp;Yoo Jin Lee,&nbsp;Jeong Eun Shin,&nbsp;Hye-Kyung Jung,&nbsp;Seon-Young Park,&nbsp;Seung Joo Kang,&nbsp;Kyung Ho Song,&nbsp;Jung-Wook Kim,&nbsp;Hyun Chul Lim,&nbsp;Hee Sun Park,&nbsp;Seong-Jung Kim,&nbsp;Ra Ri Cha,&nbsp;Ki Bae Bang,&nbsp;Chang Seok Bang,&nbsp;Sung Kyun Yim,&nbsp;Seung-Bum Ryoo,&nbsp;Bong Hyeon Kye,&nbsp;Woong Bae Ji,&nbsp;Miyoung Choi,&nbsp;In-Kyung Sung,&nbsp;Suck Chei Choi","doi":"10.5056/jnm23066","DOIUrl":"https://doi.org/10.5056/jnm23066","url":null,"abstract":"<p><p>Chronic constipation is one of the most common digestive diseases encountered in clinical practice. Constipation manifests as a variety of symptoms, such as infrequent bowel movements, hard stools, feeling of incomplete evacuation, straining at defecation, a sense of anorectal blockage during defecation, and use of digital maneuvers to assist defecation. During the diagnosis of chronic constipation, the Bristol Stool Form Scale, colonoscopy, and a digital rectal examination are useful for objective symptom evaluation and differential diagnosis of secondary constipation. Physiological tests for functional constipation have complementary roles and are recommended for patients who have failed to respond to treatment with available laxatives and those who are strongly suspected of having a defecatory disorder. As new evidence on the diagnosis and management of functional constipation emerged, the need to revise the previous guideline was suggested. Therefore, these evidence-based guidelines have proposed recommendations developed using a systematic review and meta-analysis of the treatment options available for functional constipation. The benefits and cautions of new pharmacological agents (such as lubiprostone and linaclotide) and conventional laxatives have been described through a meta-analysis. The guidelines consist of 34 recommendations, including 3 concerning the definition and epidemiology of functional constipation, 9 regarding diagnoses, and 22 regarding managements. Clinicians (including primary physicians, general health professionals, medical students, residents, and other healthcare professionals) and patients can refer to these guidelines to make informed decisions regarding the management of functional constipation.</p>","PeriodicalId":16543,"journal":{"name":"Journal of Neurogastroenterology and Motility","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/98/2d/jnm-29-3-271.PMC10334201.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10269014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnic Differences in Anorectal Manometry Findings in Patients With Fecal Incontinence: Results From a Multiethnic Cohort According to the London Classification.","authors":"Daniel L Cohen,&nbsp;Amir Mari,&nbsp;Anton Bermont,&nbsp;Dana Zelnik Yovel,&nbsp;Vered Richter,&nbsp;Haim Shirin","doi":"10.5056/jnm21233","DOIUrl":"https://doi.org/10.5056/jnm21233","url":null,"abstract":"<p><strong>Background/aims: </strong>Clinical rates of fecal incontinence (FI) are known to vary based on race and ethnicity. It is unclear if anorectal manometry (ARM) findings in patients with FI differ based on ethnicity.</p><p><strong>Methods: </strong>High-resolution ARM studies performed between 2014-2021 due to FI at 2 hospitals with multiethnic populations were retrospectively reviewed.</p><p><strong>Results: </strong>Four hundred and seventy-nine subjects were included--87 (18.2%) Arab Israelis, 76 (15.9%) immigrants from the former Soviet Union, and 316 (66.0%) Jewish Israelis. Median age was 67 years old (76.0% women: 90.4% were parous). The Arab Israeli group had higher rates of smoking, diabetes, and obesity. Over 95% of ARM's were abnormal per the London classification including 23% with \"combined anal hypotension and hypocontractility,\" 36% with \"anal normotension with anal hypocontractility,\" 67% with \"dyssynergia,\" and 65% with either \"rectal hyposensation\" or \"borderline rectal hyposensation.\" On univariate analyses, significant differences between the ethnic groups were noted in the rates of \"anal hypotension with normal contractility,\" \"combined anal hypotension with anal hypocontractility,\" and \"dyssynergia.\" In multivariate logistic regression analyses controlling for age, gender, parity, smoking, diabetes, and obesity, the Arab Israeli group remained several times more likely to have \"combined anal hypotension and hypocontractibility\" compared to the other groups.</p><p><strong>Conclusions: </strong>Ethnicity impacts ARM findings in patients with FI. The reason for this is unclear and future studies on ethnically diverse populations evaluating the clinical relevance of these findings are warranted.</p>","PeriodicalId":16543,"journal":{"name":"Journal of Neurogastroenterology and Motility","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/95/6b/jnm-29-3-370.PMC10334202.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9892352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is the Diagnostic Accuracy of Chicago Classification Version 4.0 and the Difference From Version 3.0 in Diagnosing Esophageal Motility Disorders?","authors":"Ju Yup Lee","doi":"10.5056/jnm23082","DOIUrl":"https://doi.org/10.5056/jnm23082","url":null,"abstract":"of","PeriodicalId":16543,"journal":{"name":"Journal of Neurogastroenterology and Motility","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ea/c2/jnm-29-3-269.PMC10334200.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9892348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proton Pump Inhibitor-unresponsive Laryngeal Symptoms Are Associated With Psychological Comorbidities and Sleep Disturbance: A Manometry and Impedance-pH Monitoring Study","authors":"Wen-Hsuan Tseng,&nbsp;Wei-Chung Hsu,&nbsp;Tsung-Lin Yang,&nbsp;Tzu-Yu Hsiao,&nbsp;Jia-Feng Wu,&nbsp;Hui-Chuan Lee,&nbsp;Hsiu-Po Wang,&nbsp;Ming-Shiang Wu,&nbsp;Ping-Huei Tseng","doi":"10.5056/jnm22099","DOIUrl":"https://doi.org/10.5056/jnm22099","url":null,"abstract":"<p><strong>Background/aims: </strong>Laryngeal symptoms are largely treated with empiric proton pump inhibitor (PPI) therapy if no apparent pathology shown on ear, nose, and throat evaluation and reflux-related etiologies are suspected. However, treatment response remains unsatisfactory. This study aimed to investigate the clinical and physiological characteristics of patients with PPI-refractory laryngeal symptoms.</p><p><strong>Methods: </strong>Patients with persistent laryngeal symptoms despite PPI treatment for ≥ 8 weeks were recruited. A multidisciplinary evaluation comprising validated questionnaires for laryngeal symptoms (reflux symptom index [RSI]), gastroesophageal reflux disease symptoms, psychological comorbidity (5-item brief symptom rating scale [BSRS-5]) and sleep disturbance (Pittsburgh sleep quality index [PSQI]), esophagogastroduodenoscopy, ambulatory impedance-pH monitoring, and high-resolution impedance manometry were performed. Healthy asymptomatic individuals were also recruited for comparison of psychological morbidity and sleep disturbances.</p><p><strong>Results: </strong>Ninety-seven adult patients and 48 healthy volunteers were analyzed. The patients had markedly higher prevalence of psychological distress (52.6% vs 2.1%, <i>P</i> < 0.001) and sleep disturbance (82.5% vs 37.5%, <i>P</i> < 0.001) than the healthy volunteers. There were significant correlations between RSI and BSRS-5 scores, and between RSI and PSQI scores (<i>r</i> = 0.26, <i>P</i> = 0.010, and <i>r</i> = 0.29, <i>P</i> = 0.004, respectively). Fifty-eight patients had concurrent gastroesophageal reflux disease symptoms. They had more prominent sleep disturbances (89.7% vs 71.8%, <i>P</i> < 0.001) than those with laryngeal symptoms alone but similar reflux profiles and esophageal motility.</p><p><strong>Conclusions: </strong>PPI-refractory laryngeal symptoms are mostly associated with psychological comorbidities and sleep disturbances. Recognition of these psychosocial comorbidities may help optimize management in these patients.</p>","PeriodicalId":16543,"journal":{"name":"Journal of Neurogastroenterology and Motility","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d7/be/jnm-29-3-314.PMC10334203.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9892350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Randomized Controlled Trial of Anti-reflux Mucosectomy Versus Radiofrequency Energy Delivery for Proton Pump Inhibitor-refractory Gastroesophageal Reflux Disease.","authors":"Yan Wang,&nbsp;Meihui Lv,&nbsp;Lin Lin,&nbsp;Liuqin Jiang","doi":"10.5056/jnm21240","DOIUrl":"https://doi.org/10.5056/jnm21240","url":null,"abstract":"<p><strong>Background/aims: </strong>The efficacy and safety of anti-reflux mucosectomy (ARMS) or radiofrequency energy delivery in the treatment of gastroesophageal reflux disease (GERD) have been reported, but the difference between the 2 remains unclear.</p><p><strong>Methods: </strong>This was a single center, randomized, comparative clinical study. Patients with symptoms of heartburn and/or regurgitation despite proton pump inhibitor treatment were randomly assigned to either ARMS group (n = 20) or radiofrequency group (n = 20). Primary outcome was the standardized GERD questionnaire (GERDQ) at 2 years after the procedures. Secondary outcomes were the proportions of patients with complete proton pump inhibitor (PPI) cessation and patients satisfied with the treatment.</p><p><strong>Results: </strong>A total of 18 patients randomized to ARMS and 16 to radiofrequency were analyzed in this study. The operation success rate of the 2 groups was 100%. In both ARMS and radiofrequency groups, GERDQ scores at 2 years after the procedures were significantly lower than that before operation (<i>P</i> = 0.044 and <i>P</i> = 0.046). At 2 years postoperatively, the scores of GERDQ did not differ between the 2 groups (<i>P</i> = 0.755). There was no significant difference in the rate of discontinuation of PPIs and patient satisfaction in the ARMS and radiofrequency groups (<i>P</i> = 0.642 and <i>P</i> = 0.934).</p><p><strong>Conclusions: </strong>The clinical efficacy of ARMS and radiofrequency for the PPI-refractory GERD is equivalent. ARMS, the efficacy of which could be maintained for at least 2 years, is promising endoscopic management for the treatment of refractory GERD.</p>","PeriodicalId":16543,"journal":{"name":"Journal of Neurogastroenterology and Motility","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fd/82/jnm-29-3-306.PMC10334194.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10156497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Diagnosis of Esophageal Motility Disorders by Chicago Classification Versions 3.0 and 4.0.","authors":"Jin Hee Noh,&nbsp;Kee Wook Jung,&nbsp;In Ja Yoon,&nbsp;Hee Kyong Na,&nbsp;Ji Yong Ahn,&nbsp;Jeong Hoon Lee,&nbsp;Do Hoon Kim,&nbsp;Kee Don Choi,&nbsp;Ho June Song,&nbsp;Gin Hyug Lee,&nbsp;Hwoon-Yong Jung","doi":"10.5056/jnm22121","DOIUrl":"https://doi.org/10.5056/jnm22121","url":null,"abstract":"<p><strong>Background/aims: </strong>We aim to investigate the diagnostic accuracy and differences between Chicago classification version 3.0 (CC v3.0) and 4.0 (CC v4.0).</p><p><strong>Methods: </strong>Patients who underwent high-resolution esophageal manometry (HRM) for suspected esophageal motility disorders were prospectively recruited between May 2020 and February 2021. The protocol of HRM studies included additional positional change and provocative testing designed by CC v4.0.</p><p><strong>Results: </strong>Two hundred forty-four patients were included. The median age was 59 (interquartile range, 45-66) years, and 46.7% were males. Of these, 53.3% (n = 130) and 61.9% (n = 151) were categorized as normalcy by CC v3.0 and CC v4.0, respectively. The 15 patients diagnosed of esophagogastric junction outflow obstruction (EGJOO) by CC v3.0 was changed to normalcy by position (n = 2) and symptom (n = 13) by CC v4.0. In seven patients, the ineffective esophageal motility (IEM) diagnosis by CC v3.0 was changed to normalcy by CC v4.0. The diagnostic rate of achalasia increased from 11.1% (n = 27) to 13.9% (n = 34) by CC v4.0. Of patients diagnosed IEM by CC v3.0, 4 was changed to achalasia based on the functional lumen imaging probe (FLIP) results by CC v4.0. Three patients (2 with absent contractility and 1 with IEM in CC v3.0) were newly diagnosed with achalasia using a provocative test and barium esophagography by CC v4.0.</p><p><strong>Conclusions: </strong>CC v4.0 is more rigorous than CC v3.0 for the diagnosis of EGJOO and IEM and diagnoses achalasia more accurately by using provocative tests and FLIP. Further studies on the treatment outcomes following diagnosis with CC v4.0 are needed.</p>","PeriodicalId":16543,"journal":{"name":"Journal of Neurogastroenterology and Motility","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/05/74/jnm-29-3-326.PMC10334199.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9883379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stigma and Efficacy of Zhizhu Kuanzhong Capsules Versus Doxepin in the Treatment of Refractory Functional Dyspepsia: A Randomized Controlled Trial.","authors":"Qian-Qian Wang,&nbsp;Li Cheng,&nbsp;Bi-Yu Wu,&nbsp;Hong-Yi Qiu,&nbsp;Ping Xu,&nbsp;Bo Wang,&nbsp;Xiu-Juan Yan,&nbsp;Sheng-Liang Chen","doi":"10.5056/jnm22145","DOIUrl":"https://doi.org/10.5056/jnm22145","url":null,"abstract":"<p><strong>Background/aims: </strong>Stigma related with antidepressants is prevalent in patients with functional dyspepsia. It affects medication compliance and efficacy. Herbal medicine acquired a deep-rooted cultural identity in relieving dyspeptic symptoms in Asians. The research was designed to compare the effectiveness of Zhizhu Kuanzhong capsules (ZZKZ) versus doxepin hydrochloride (doxepin) on alleviating stigma and medication nonadherence among patients with refractory FD (rFD).</p><p><strong>Methods: </strong>Patients with rFD from February 2021 to February 2022 were randomly allocated to receive either doxepin (n = 56) or ZZKZ (n = 57) combined with omeprazole for 4 weeks. Medication possession ratio (MPR), the disease- and medication-associated stigma were analyzed. The scales were utilized to assess dyspeptic symptoms (Leeds Dyspepsia Questionnaire) and psychological conditions (Generalized Anxiety Disorder Questionnaire and Patient Health Questionnaire).</p><p><strong>Results: </strong>The MPR values for ZZKZ were significantly higher than those for doxepin (<i>P</i> < 0.001). The stigma scores decreased in ZZKZ group while increased in doxepin group compared to baseline after treatment. The proportion of patients showing ZZKZ-associated stigma was significantly lower than doxepin-associated stigma (<i>P</i> < 0.001). The MPR values were negatively correlated with post-treatment stigma scores in both groups (<i>P</i> < 0.001). Dyspeptic symptoms and psychological condition were improved in both groups after treatment, with no significant difference on post-treatment Leeds Dyspepsia Questionnaire, Generalized Anxiety Disorder Questionnaire, or Patient Health Questionnaire scores between 2 groups.</p><p><strong>Conclusion: </strong>ZZKZ is superior to doxepin in alleviating stigma and medication non-adherence, with comparable efficacy in improving dyspeptic symptoms and psychological condition of patients with rFD.</p>","PeriodicalId":16543,"journal":{"name":"Journal of Neurogastroenterology and Motility","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/65/95/jnm-29-3-360.PMC10334204.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10269009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autonomic Dysfunction and Somatization in Young Patients With Irritable Bowel Syndrome and Mitral Valve Prolapse Syndrome.","authors":"Jannis Kountouras,&nbsp;Apostolis Papaefthymiou,&nbsp;Stergios A Polyzos,&nbsp;Elisabeth Vardaka,&nbsp;Foteini Kyrailidi,&nbsp;Maria C Mouratidou,&nbsp;Christos Zavos,&nbsp;Evangelos Kazakos,&nbsp;Dimitrios Chatzopoulos,&nbsp;Maria Tzitiridou-Chatzopoulou,&nbsp;Dimitrios Tzilves,&nbsp;Christos Liatsos,&nbsp;Maria Touloumtzi,&nbsp;Michael Doulberis","doi":"10.5056/jnm23061","DOIUrl":"https://doi.org/10.5056/jnm23061","url":null,"abstract":"","PeriodicalId":16543,"journal":{"name":"Journal of Neurogastroenterology and Motility","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/44/5c/jnm-29-3-403.PMC10334193.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10269012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Optogenetics-based Approach to Regulate Colonic Contractions by Modulating the Activity of the Interstitial Cells of Cajal in Mice.","authors":"Song Zhao,&nbsp;Weidong Tong","doi":"10.5056/jnm22181","DOIUrl":"https://doi.org/10.5056/jnm22181","url":null,"abstract":"<p><strong>Background/aims: </strong>The interstitial cells of Cajal (ICC) are pacemaker cells in the gastrointestinal (GI) tract. We examined whether the activity of ICC could be stimulated to control colonic contractions. An optogenetics-based mouse model in which the light-sensitive protein channelrhodopsin-2 (ChR2) was expressed was used to accomplish cell specific, direct stimulation of ICC.</p><p><strong>Methods: </strong>An inducible site-specific Cre-loxP recombination system was used to generate <i>Kit^CreERT2/+</i>;<i>ROSA^{ChR2(H134R)/tdTomato/+}</i> mice in which ChR2(H134R), a variant of ChR2, was genetically expressed in ICC after tamoxifen administration. Genotyping and immunofluorescence analysis were performed to confirm gene fusion and expression. Isometric force recordings were performed to measure changes in contractions in the colonic muscle strips.</p><p><strong>Results: </strong>ChR2 was specifically expressed in Kit-labeled ICC. The isometric force recordings showed that the contractions of the colonic muscle strips changed under 470 nm blue light. Light stimulation evoked premature low-frequency and high amplitude (LFHA) contractions and enhanced the frequency of the LFHA contractions. The light-evoked contractions were blocked by T16Ainh-A01, an antagonist of anoctamin 1 channels that are expressed selectively in ICC in colonic muscles.</p><p><strong>Conclusions: </strong>Our study demonstrates a potentially feasible approach to stimulate the activity of ICC by optogenetics. The colonic motor patterns of muscle strips, especially LFHA contractions, can be regulated by 470 nm light via ChR2, which is expressed in ICC.</p>","PeriodicalId":16543,"journal":{"name":"Journal of Neurogastroenterology and Motility","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/90/9b/jnm-29-3-388.PMC10334192.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10269013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}