Journal of microscopy最新文献_第6页

TOC - Issue Information TOC - 发行信息

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-15 DOI: 10.1111/jmi.13198

引用次数: 0

Surface passivation and functionalisation for mass photometry 用于质量光度测量的表面钝化和功能化技术

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-12 DOI: 10.1111/jmi.13302

Jenny Sülzle, Laila Elfeky, Suliana Manley

引用次数: 0

Sulphate resistance of low-clinker engineered cementitious composites examined by MicroXRF imaging 通过 MicroXRF 成像检测低熟料工程水泥基复合材料的抗硫酸盐性能

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-10 DOI: 10.1111/jmi.13303

Connor Szeto, Kimberly E. Kurtis

{"title":"Sulphate resistance of low-clinker engineered cementitious composites examined by MicroXRF imaging","authors":"Connor Szeto, Kimberly E. Kurtis","doi":"10.1111/jmi.13303","DOIUrl":"https://doi.org/10.1111/jmi.13303","url":null,"abstract":"Engineered cementitious composites (ECC) are a class of high-performing fibre-reinforced cementitious materials recognised for their increased ductility and durability compared to conventional cement-based materials, owing to their autogenously controlled tight crack widths, even when subjected to high strains. To reduce ECC's environmental impact, this research examines the use of a low-clinker binder − limestone-calcined clay cement (LC3) − as an alternative to portland cement (PC), along with fly ash to further reduce the clinker proportion and the embodied CO2 of the formulations. In conventional concrete, LC3 hydrates to a denser microstructure resulting from the synergistic reaction between limestone and calcined clay. At the lower water contents typical of ECC and with the presence of fly ash, the influence of the binder composition on the microstructure is difficult to anticipate.To examine the influence of these compositional variables on microstructure, permeability and durability, the sulphate resistance of LC3-based ECC is explored. Specifically, the ECC-LC3 blends are designed with high clinker replacement rate of 75% by mass of binder and contain either conventional fly ash or reclaimed fly ash at 50% by mass of binder. Expansion of ECC-LC3 samples subjected to standard sodium sulphate test conditions was measured up to 12 months and the depth of penetration of sulphates into the ECC-LC3 of varying compositions was quantified using micro-X-Ray Fluorescence (microXRF) imaging and modelling. The expansion results show that the ECC-LC3 formulations performed better than the PC samples and can provide adequate resistance to external sulphate attack, even when reclaimed fly ashes are used in place of the conventional ash. In addition, the shallow penetration of sulphate into these cementitious composites demonstrates the low diffusion coefficients values that were determined using the quantitative data from MicroXRF imaging.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13303","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140556339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introduction for special issue: 19th Euroseminar on Microscopy Applied to Building Materials (EMABM) 2024 特刊简介：第 19 届欧洲建筑材料应用显微镜研讨会 (EMABM) 2024

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-10 DOI: 10.1111/jmi.13299

Alexander Wetzel

引用次数: 0

Studying crystallisation processes using electron microscopy: The importance of sample preparation 使用电子显微镜研究结晶过程：样品制备的重要性

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2024-04-09 DOI: 10.1111/jmi.13300

Martha Ilett, Maryam Afzali, Bilal Abdulkarim, Zabeada Aslam, Stephanie Foster, Miguel Burgos-Ruiz, Yi-Yeoun Kim, Fiona C. Meldrum, Rik M. Drummond-Brydson

引用次数: 0

Characterisation of bond between cement paste and steel fibres with different surface roughness using SEM 利用扫描电子显微镜分析不同表面粗糙度的水泥浆与钢纤维之间的粘结特性

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-05 DOI: 10.1111/jmi.13298

Anna Antonova, Marika Eik, Jari Puttonen

引用次数: 0

Quantification of cell shape, intracellular flows and transport based on DIC object detection and tracking 基于 DIC 物体检测和跟踪的细胞形状、细胞内流动和运输定量分析

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-04 DOI: 10.1111/jmi.13295

Tanvi Kale, Dhruv Khatri, Jashaswi Basu, Shivani A. Yadav, Chaitanya A. Athale

{"title":"Quantification of cell shape, intracellular flows and transport based on DIC object detection and tracking","authors":"Tanvi Kale, Dhruv Khatri, Jashaswi Basu, Shivani A. Yadav, Chaitanya A. Athale","doi":"10.1111/jmi.13295","DOIUrl":"https://doi.org/10.1111/jmi.13295","url":null,"abstract":"Computational image analysis combined with label‐free imaging has helped maintain its relevance for cell biology, despite the rapid technical improvements in fluorescence microscopy with the molecular specificity of tags. Here, we discuss some computational tools developed in our lab and their application to quantify cell shape, intracellular organelle movement and bead transport in vitro, using differential interference contrast (DIC) microscopy data as inputs. The focus of these methods is image filtering to enhance image gradients, and combining them with segmentation and single particle tracking (SPT). We demonstrate the application of these methods to Escherichia coli cell length estimation and tracking of densely packed lipid granules in Caenorhabditis elegans one‐celled embryos, diffusing beads in solutions of different viscosities and kinesin‐driven transport on microtubules. These approaches demonstrate how improvements to low‐level image analysis methods can help obtain insights through quantitative cellular and subcellular microscopy.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing resolution and contrast in fibre bundle-based fluorescence microscopy using generative adversarial network 利用生成式对抗网络提高基于纤维束的荧光显微镜的分辨率和对比度。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2024-04-02 DOI: 10.1111/jmi.13296

Amir Mohammad Ketabchi, Berna Morova, Yiğit Uysalli, Musa Aydin, Furkan Eren, Nima Bavili, Dariusz Pysz, Ryszard Buczynski, Alper Kiraz

{"title":"Enhancing resolution and contrast in fibre bundle-based fluorescence microscopy using generative adversarial network","authors":"Amir Mohammad Ketabchi, Berna Morova, Yiğit Uysalli, Musa Aydin, Furkan Eren, Nima Bavili, Dariusz Pysz, Ryszard Buczynski, Alper Kiraz","doi":"10.1111/jmi.13296","DOIUrl":"10.1111/jmi.13296","url":null,"abstract":"Fibre bundle (FB)-based endoscopes are indispensable in biology and medical science due to their minimally invasive nature. However, resolution and contrast for fluorescence imaging are limited due to characteristic features of the FBs, such as low numerical aperture (NA) and individual fibre core sizes. In this study, we improved the resolution and contrast of sample fluorescence images acquired using in-house fabricated high-NA FBs by utilising generative adversarial networks (GANs). In order to train our deep learning model, we built an FB-based multifocal structured illumination microscope (MSIM) based on a digital micromirror device (DMD) which improves the resolution and the contrast substantially compared to basic FB-based fluorescence microscopes. After network training, the GAN model, employing image-to-image translation techniques, effectively transformed wide-field images into high-resolution MSIM images without the need for any additional optical hardware. The results demonstrated that GAN-generated outputs significantly enhanced both contrast and resolution compared to the original wide-field images. These findings highlight the potential of GAN-based models trained using MSIM data to enhance resolution and contrast in wide-field imaging for fibre bundle-based fluorescence microscopy.Lay Description: Fibre bundle (FB) endoscopes are essential in biology and medicine but suffer from limited resolution and contrast for fluorescence imaging. Here we improved these limitations using high-NA FBs and generative adversarial networks (GANs). We trained a GAN model with data from an FB-based multifocal structured illumination microscope (MSIM) to enhance resolution and contrast without additional optical hardware. Results showed significant enhancement in contrast and resolution, showcasing the potential of GAN-based models for fibre bundle-based fluorescence microscopy.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of the combined addition of copper, lithium and sulphur on the formation of Portland cement clinker 铜、锂和硫的联合添加对波特兰水泥熟料形成的影响。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-01 DOI: 10.1111/jmi.13293

Theodor Staněk, Alexandra Rybová, Martin Boháč, Eva Bartoníčková, Jakub Palovčík

{"title":"The effect of the combined addition of copper, lithium and sulphur on the formation of Portland cement clinker","authors":"Theodor Staněk, Alexandra Rybová, Martin Boháč, Eva Bartoníčková, Jakub Palovčík","doi":"10.1111/jmi.13293","DOIUrl":"10.1111/jmi.13293","url":null,"abstract":"Both copper and lithium act as strong fluxes and lower the temperature of the clinker melt formation. Sulphur promotes the stabilisation of more hydraulically active modification of alite M1. It is expected that this combination could produce an alite clinker at significantly lower temperatures with high quality technological parameters. In this paper, the effect of combined oxides of copper, lithium and sulphur addition on the phase composition and clinker structure of Portland cement was investigated. The reference raw meal was prepared from common cement raw materials. Each of the mentioned oxides was added to the reference raw meal in two different concentrations, and 8 combinations were prepared. Chemically pure compounds (NH4)2SO4, CuO and Li2CO3 were used as a source of these oxides. The raw meals were burned to equilibrium at 1450°C. Their phase composition was determined by X-ray diffraction analysis, the microstructure was monitored by optical microscopy, and the microchemistry of the clinker phases was observed by electron microscopy with EDS analysis. It was found that in samples with high lithium or copper content, there is an increase in belite and free lime at the expense of alite. The combination of Cu + Li has the most negative effect, followed by Li alone and Cu alone. The higher SO3 content slightly offsets this negative effect.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence microscopic investigation of PCE superplasticiser adsorption in calcined clay blended cement 煅烧粘土混合水泥中 PCE 超塑化剂吸附的荧光显微镜研究。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-03-31 DOI: 10.1111/jmi.13294

D. Kosenko, A. Wetzel, B. Middendorf

引用次数: 0