Journal of nematologyPub Date : 2023-11-15eCollection Date: 2023-02-01DOI: 10.2478/jofnem-2023-0031
Ashish Kumar Singh, Antara Das, Ila Joshi, Manish Kumar, Deshika Kohli, Pankaj, Kishor Gaikwad, Pradeep Kumar Jain, Anil Sirohi
{"title":"Nematode Genome Announcement: A Draft Genome of Seed Gall Nematode, <i>Anguina tritici</i>.","authors":"Ashish Kumar Singh, Antara Das, Ila Joshi, Manish Kumar, Deshika Kohli, Pankaj, Kishor Gaikwad, Pradeep Kumar Jain, Anil Sirohi","doi":"10.2478/jofnem-2023-0031","DOIUrl":"10.2478/jofnem-2023-0031","url":null,"abstract":"<p><p><i>Anguina tritici</i> is the first plant-parasitic nematode described in literature, dating back to the year 1743. It is responsible for causing earcockle (seed gall) and tundu diseases in wheat and rye. Notably, this nematode has been observed to survive in an anhydrobiotic state for up to 32 years within wheat seed galls. These exceptional characteristics have inspired the sequencing of the <i>A. tritici</i> genome. In this study, we present the initial draft genome of <i>A. tritici</i>, obtained using the Illumina MiSeq platform with coverage of 60-fold. The genome is estimated to have a size of 164 Mb and comprises 39,965 protein-coding genes, exhibiting a GC content of 39.1%. The availability of this genome data will serve as a foundation for future functional biological investigations, particularly for genes whose functions remain unknown to this day.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230031"},"PeriodicalIF":1.3,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138460477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A New Root-Knot Nematode species, <i>Meloidogyne karsseni</i> n. sp. (Nematoda: Meloidogynidae), From Mexico and a Taxonomic Update on <i>M. paranaensis</i> From Guatemala.","authors":"Phougeishangbam Rolish Singh, Denis Gitonga, Abolfazl Hajihassani, Adriaan Verhage, Eveline van Aalst-Philipse, Marjolein Couvreur, Wim Bert","doi":"10.2478/jofnem-2023-0042","DOIUrl":"10.2478/jofnem-2023-0042","url":null,"abstract":"<p><p>A new root-knot nematode (RKN) species, <i>Meloidogyne karsseni</i> n. sp., associated with sweet pepper from Mexico, and a population of <i>M. paranaensis</i> from Guatemala, are described using data from morphological, biochemical (isozyme enzymes), molecular, and phylogenetic analyses. <i>Meloidogyne karsseni</i> n. sp. can be morphologically diagnosed using the combined features of the second-stage juveniles, viz. body length (345 to 422 μm), a conical rounded head region, a post-labial annule lacking transverse striation, a thin stylet 11 to 12 μm long, rounded to oval and backwardly sloping knobs, dorsal gland orifice (DGO) at 5.2 to 6.0 μm from the knobs, a hemizonid just above the secretory-excretory (SE) pore, a tapering tail with finely rounded terminus and one or two very weak constrictions at hyaline tail tip; the female characters viz. oval-to-rounded perineal pattern with coarse striation on lateral sides around the anus, low dorsal arch with finer striations, and distinctly visible lateral lines; and the male characteristics viz. a rounded and continuous head, a post-labial annule without transverse striations, a robust stylet 20 to 24 μm long, rounded-to-oval and slightly backwardly sloping knobs, and a DGO at 2.4 to 2.9 μm from the knobs. In all the studied males of <i>M. paranaensis</i>, a characteristic sclerotization around the duct of SE-pore was also observed for the first time. Sequences of 18S, D2-D3 of 28S, and ITS of rDNA, and <i>cox</i>1 of mtDNA were generated for the two species, and in the phylogenetic trees based on these genes, both species appeared in the tropical RKN species complex clade.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230042"},"PeriodicalIF":1.3,"publicationDate":"2023-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49690973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of nematologyPub Date : 2023-10-21eCollection Date: 2023-02-01DOI: 10.2478/jofnem-2023-0041
Rebeca Sandoval-Ruiz, Zane J Grabau
{"title":"Management of Reniform Nematode in Cotton Using Winter Crop Residue Amendments Under Greenhouse Conditions.","authors":"Rebeca Sandoval-Ruiz, Zane J Grabau","doi":"10.2478/jofnem-2023-0041","DOIUrl":"10.2478/jofnem-2023-0041","url":null,"abstract":"<p><p><i>Rotylenchulus reniformis</i> (reniform nematode, RN) is among the most important nematodes affecting cotton. Cultural practices, such as rotation and soil amendment, are established methods for managing RN. Management may be enhanced if crop residue has biofumigant properties against RN. The objective was to evaluate the efficacy of winter crop amendments for managing RN in the greenhouse. Reniform nematode-infested soil was amended with dry or fresh organic matter (OM, 2% w/w) from winter crops - canola, carinata, hairy vetch, oat, or no crop. Cotton was subsequently grown in this soil. Independent of the crop, dry OM amendments were more effective than no amendment at managing RN, while fresh OM amendments were not. Soil and root RN abundances and reproduction factors were generally lower in Trials 1 and 3 for dry OM than fresh OM amendments or control without OM. In Trial 2, none of the OM treatments reduced RN parameters compared with no OM control. In general, when compared to plants without RN or OM, RN did not produce significant changes in growth parameters but did affect physiology (Soil Plant Analysis Development, or SPAD, values). In conclusion, dry OM amendments can help manage RN, crop growth does not always relate to RN abundances, and SPAD values could help indicate RN presence.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230041"},"PeriodicalIF":1.4,"publicationDate":"2023-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49690974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of nematologyPub Date : 2023-10-16eCollection Date: 2023-02-01DOI: 10.2478/jofnem-2023-0043
A T Ploeg, C S Stoddard, T A Turini, J J Nunez, E M Miyao, S A Subbotin
{"title":"Tomato <i>Mi</i>-gene Resistance-Breaking Populations of <i>Meloidogyne</i> Show Variable Reproduction on Susceptible and Resistant Crop Cultivars.","authors":"A T Ploeg, C S Stoddard, T A Turini, J J Nunez, E M Miyao, S A Subbotin","doi":"10.2478/jofnem-2023-0043","DOIUrl":"10.2478/jofnem-2023-0043","url":null,"abstract":"<p><p>Sixteen <i>Meloidogyne</i> isolates from tomato fields in California grown with resistant cultivars were multiplied on resistant tomato in a greenhouse. Of these resistance-breaking isolates, one was identified as <i>M. javanica</i>, and all others as <i>M. incognita.</i> The reproduction of the <i>M. javanica</i> isolate and four <i>M. incognita</i> isolates on six resistant tomato cultivars and on susceptible and resistant cultivars of pepper, sweetpotato, green bean, cotton, and cowpea was evaluated and compared to an avirulent <i>M. incognita</i> population in greenhouse pot trials. On resistant tomato cultivars, there were minor but significant differences between the resistance-breaking <i>Meloidogyne</i> isolates and between the different tomato cultivars. Of the other resistant crop cultivars, pepper was resistant to all isolates and green bean to all <i>M. incognita</i> isolates, while cotton and cowpea allowed reproduction of one of the resistance-breaking <i>M. incognita</i> isolates. The resistant sweetpotato cv. Bonita behaved like resistant tomato, allowing reproduction of all five resistance-breaking isolates but not of the avirulent <i>M. incognita</i>. Our results showed that variability exists among resistance-breaking <i>Meloidogyne</i> isolates, and that isolates overcoming resistance in tomato may also be virulent on resistant sweetpotato.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230043"},"PeriodicalIF":1.4,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41236095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of nematologyPub Date : 2023-10-16eCollection Date: 2023-02-01DOI: 10.2478/jofnem-2023-0045
Sahil Agarwal, Zachary C Curran, Guohao Yu, Shova Mishra, Anil Baniya, Mesfin Bogale, Kody Hughes, Oscar Salichs, Alina Zare, Zhe Jiang, Peter DiGennaro
{"title":"Plant Parasitic Nematode Identification in Complex Samples with Deep Learning.","authors":"Sahil Agarwal, Zachary C Curran, Guohao Yu, Shova Mishra, Anil Baniya, Mesfin Bogale, Kody Hughes, Oscar Salichs, Alina Zare, Zhe Jiang, Peter DiGennaro","doi":"10.2478/jofnem-2023-0045","DOIUrl":"10.2478/jofnem-2023-0045","url":null,"abstract":"<p><p>Plant parasitic nematodes are significant contributors to yield loss worldwide, causing devastating losses to every crop species, in every climate. Mitigating these losses requires swift and informed management strategies, centered on identification and quantification of field populations. Current plant parasitic nematode identification methods rely heavily on manual analyses of microscope images by a highly trained nematologist. This mode is not only expensive and time consuming, but often excludes the possibility of widely sharing and disseminating results to inform regional trends and potential emergent issues. This work presents a new public dataset containing annotated images of plant parasitic nematodes from heterologous soil extractions. This dataset serves to propagate new automated methodologies or speedier plant parasitic nematode identification using multiple deep learning object detection models and offers a path towards widely shared tools, results, and meta-analyses.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230045"},"PeriodicalIF":1.4,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41236093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of nematologyPub Date : 2023-10-16eCollection Date: 2023-02-01DOI: 10.2478/jofnem-2023-0047
{"title":"Society of Nematologists 1961.","authors":"","doi":"10.2478/jofnem-2023-0047","DOIUrl":"10.2478/jofnem-2023-0047","url":null,"abstract":"","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230047"},"PeriodicalIF":1.3,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41236094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of nematologyPub Date : 2023-10-04eCollection Date: 2023-02-01DOI: 10.2478/jofnem-2023-0039
Ricky Critchfield, Jaden King, John Bonkowski, Darcy Telenko, Tom Creswell, Lei Zhang
{"title":"Characterization of Virulence Phenotypes of <i>Heterodera glycines</i> during 2020 in Indiana.","authors":"Ricky Critchfield, Jaden King, John Bonkowski, Darcy Telenko, Tom Creswell, Lei Zhang","doi":"10.2478/jofnem-2023-0039","DOIUrl":"10.2478/jofnem-2023-0039","url":null,"abstract":"<p><p>The soybean cyst nematode (SCN, <i>Heterodera glycines</i>) is the most yield-limiting pathogen of soybean in the US. This study was carried out in order to provide updated information on SCN virulence phenotypes in Indiana. A total of 124 soil samples were collected from soybean fields in 2020 and all of them tested positive for SCN. The virulence phenotypes of 42 representative SCN populations were determined with seven soybean indicator lines using the standard HG type test. The most predominant HG types were 2.5.7 and 1.2.5.7, which accounted for 64% and 14% of the SCN populations tested, respectively. None of the SCN populations tested were rated as HG type 0, compared with 28% of the populations in a previous survey in Indiana during 2006-2008. Nearly 88% of the SCN populations evaluated in this study overcame the resistance provided by PI 88788, which is the most common source of resistance in soybean, up from 56% in the 2006-2008 survey. Approximately 14% of SCN populations tested were virulent to PI 548402 (Peking), in contrast to 0% in the 2006-2008 survey. This study reveals a trend of increasing virulence of SCN populations to resistant sources of soybean in Indiana. The results highlighted the importance of rotating soybean varieties with different types of resistance and identifying new sources of resistance for sustainable management of SCN.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230039"},"PeriodicalIF":1.3,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10577647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41236092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of nematologyPub Date : 2023-09-13eCollection Date: 2023-02-01DOI: 10.2478/jofnem-2023-0035
Rebeca Sandoval-Ruiz, Zane J Grabau
{"title":"Reniform Nematode Management Using Winter Crop Rotation and Residue Incorporation Methods in Greenhouse Experiments.","authors":"Rebeca Sandoval-Ruiz, Zane J Grabau","doi":"10.2478/jofnem-2023-0035","DOIUrl":"10.2478/jofnem-2023-0035","url":null,"abstract":"<p><p><i>Rotylenchulus reniformis</i> (reniform nematode, RN) is an important pathogen in cotton production. Cultural practices such as crop rotation and biofumigation-management of soil pathogens by biocidal compounds from crop residues-may help manage RN. The objective of this study was to evaluate the efficacy of winter crops for RN management through combinations of rotation and crop residue incorporation in a cotton greenhouse experiment. A total of 10 treatments were evaluated in soil inoculated with RN: three winter crops (carinata, oat, or hairy vetch) grown in rotation with no shoot organic matter (OM) incorporated (1-3), fresh shoot OM incorporated (4-6), or dry shoot OM incorporated (7-9), and a fallow control (10). Roots were re-incorporated in all treatments except fallow. Subsequently, cotton was grown. Oat and fallow were better rotation crops to lower soil RN abundances at winter crop termination than hairy vetch and carinata. After the OM incorporation treatments and cotton growth, oat was generally more effective at managing RN in cotton than carinata or hairy vetch. Within each crop, incorporation treatment generally did not affect RN management. Cotton growth was not consistently affected by the treatments.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230035"},"PeriodicalIF":1.3,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10272922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of nematologyPub Date : 2023-09-01eCollection Date: 2023-02-01DOI: 10.2478/jofnem-2023-0037
Renáta Petrikovszki, Ferenc Tóth, Péter I Nagy
{"title":"Aqueous Extracts of Organic Mulch Materials Have Nematicide and Repellent Effect on <i>Meloidogyne incognita</i> Infective Juveniles: A Laboratory Study.","authors":"Renáta Petrikovszki, Ferenc Tóth, Péter I Nagy","doi":"10.2478/jofnem-2023-0037","DOIUrl":"10.2478/jofnem-2023-0037","url":null,"abstract":"<p><p>While the nematicidal effectiveness of mulching against root-knot nematodes (<i>Meloidogyne</i> spp.) is calculated within organic crop protection, underlying mechanisms are not yet fully explored. Laboratory experiments were set up to determine whether mulch-derived substances cause mortality directly, or repel <i>Meloidogyne</i> juveniles from crop rhizosphere. Mortality and area choice tests were conducted with mulch-derived extracts, supported by the measurements on tannic acid content and the pH values of extracts as supplementary examinations. In our study, leaf litter and straw extracts were generally found lethal to the juveniles, which is in line with the results from area preference tests. However, compost extract had no effect on <i>Meloidogyne incognita</i> juveniles. Tannic acid content showed positive correlation with mortality only in the case of straw and sycamore leaf litter extracts. Tannic acid and pH weakly correlated with repellent effect of the applied extracts generally. Our results have inspired further experiments to explore nematicidal components of leaf litters, contributing to the development of a new approach in crop protection based on the repellent effect of these materials.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230037"},"PeriodicalIF":1.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10473840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10230446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of nematologyPub Date : 2023-08-29eCollection Date: 2023-02-01DOI: 10.2478/jofnem-2023-0030
Leonardo F Rocha, Mary E Kinsel, Jason P Bond, Ahmad M Fakhoury
{"title":"Chemical Profiles of <i>Heterodera glycines</i> Suppressive Soils in Double Cropping Soybean Production.","authors":"Leonardo F Rocha, Mary E Kinsel, Jason P Bond, Ahmad M Fakhoury","doi":"10.2478/jofnem-2023-0030","DOIUrl":"10.2478/jofnem-2023-0030","url":null,"abstract":"<p><p>We previously reported soybean fields double-cropped with winter wheat having reduced soybean cyst nematode (SCN) (<i>Heterodera glycines</i>) counts compared to fallow. A follow-up metagenomics study identified several fungal and bacterial taxa enriched in wheat fields, and some were reported to parasitize SCN. Knowing that phytocompounds with potential nematicidal activity are released via wheat roots and stubble, we implemented a dichloromethane-based extraction method and a gas chromatography-mass spectrometry (GCMS) system to investigate soil chemical profiles of samples collected from these fields and review the potential nematicidal activity of compounds with higher concentration in double cropping fields. 51 compounds were detected during the GCMS analysis, eight with unknown identification. Several compounds, including multiple fatty acids, had larger relative peak areas when double-cropped, compared to fallow samples. This study, along with our previously published one, provided a better understanding of the mechanisms that govern the effect of wheat on SCN populations. Rather than driven by a single mechanism, the suppression of SCN in soybean fields double-cropped with winter wheat was potentially linked to enriched microbial communities, increased populations of beneficial organisms, and higher concentrations of chemicals with potential nematicidal activity. To our knowledge, this is the first study using GCMS to characterize soil chemical profiles in soybean fields double-cropped with winter wheat regarding the suppression of SCN populations.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230030"},"PeriodicalIF":1.4,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41203487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}